Publications

[mycobacterium bovis in wildlife of the dairy regions of santa fe (argentina)].	control eradication campaigns of bovine tuberculosis based on the «test and slaughter» approach were successful in many countries and regions; however, in some areas the infection persists and one of the main reasons is mycobacterium bovis infection in wild life species. argentina has applied the same approach since 1999, achieving progress in dairy cattle herds. nonetheless, the wildlife role has never been investigated. the objective of this study was to determine if wildlife from the santa fe ...	2015	26376835
assessment of the probability of introduction of bovine tuberculosis to danish cattle farms via imports of live cattle from abroad and immigrant workers.	denmark has been recognized as officially free (otf) from bovine tuberculosis (btb) since 1980. in this study, we estimated the annual probability (pintro) of introducing mycobacterium bovis into the danish cattle population, through (a) imports of cattle and (b) foreign personnel working in danish cattle herds. data from 2000 to 2013 with date, number and origin of imported live cattle were obtained from the danish cattle federation. information on immigrants working in danish cattle herds was ...	2015	26409756
relationship of bovine nos2 gene polymorphisms to the risk of bovine tuberculosis in holstein cattle.	many studies suggest significant genetic variation in the resistance of cattle and humans to infection with mycobacterium bovis, the causative agent of zoonotic tuberculosis. the inducible nitric oxide synthase (inos which is encoded by the nos2 gene) plays a key role in the immunological control of a broad spectrum of infectious agents. this study aimed to investigate the influence of genetic variations in the promoter of the nos2 gene on bovine tuberculosis (btb) susceptibility. in this study, ...	2016	26468216
relationship of bovine tnf-α gene polymorphisms with the risk of bovine tuberculosis in holstein cattle.	many studies suggest significant genetic variation in the resistance of cattle and humans to infection with mycobacterium bovis (m. bovis), the causative agent of zoonotic tuberculosis. tnf-α promotes inflammation and induces apoptosis in response to mycobacterial infection. the aim of the present study was to investigate the influence of single nucleotide polymorphisms of the tnf-α gene on bovine tuberculosis (btb) susceptibility. we genotyped the tnf-α gene in 74 btb-infected holstein cows and ...	2016	26876219
molecular detection of mycobacterium bovis in cattle herds of the state of pernambuco, brazil.	the present study aimed to direct detect mycobacterium bovis in milk (n = 401) and blood (n = 401) samples collected from 401 dairy cows of 20 properties located in the state of pernambuco, brazil, by real-time quantitative pcr (qpcr) targeting the region of difference 4 (rd4). risk factors possibly associated with bovine tuberculosis (btb) were also evaluated.	2016	26897524
a genome-wide association study for genetic susceptibility to mycobacterium bovis infection in dairy cattle identifies a susceptibility qtl on chromosome 23.	bovine tuberculosis (btb) infection in cattle is a significant economic concern in many countries, with annual costs to the uk and irish governments of approximately €190 million and €63 million, respectively, for btb control. the existence of host additive and non-additive genetic components to btb susceptibility has been established.	2016	26960806
knowledge, attitudes and practices regarding risk to human infection due to mycobacterium bovis among cattle farming communities in western uganda.	a cross-sectional study involving multistage cluster sampling was undertaken in kashari county, mbarara district, western uganda, in which quantitative and qualitative approaches were utilized to determine the knowledge, attitudes and practices regarding risk of human infection with zoonotic tuberculosis due to mycobacterium bovis among cattle farmers. of 496 respondents, 50% were farm owners and 50% herdsmen. males were 70.9% of all the study participants. among these, 37.5% had good knowledge, ...	2016	27178229
responses to diagnostic tests for bovine tuberculosis in dairy and non-dairy cattle naturally exposed to mycobacterium bovis in great britain.	field surveillance of british cattle using the single intradermal comparative cervical tuberculin (sicct) test shows a higher incidence rate of bovine tuberculosis (btb) in dairy compared to beef herds, but a lower probability of post-mortem examination confirmed (pmc) mycobacterium bovis infection in dairy herds. a cross-sectional study was conducted to compare animal level differences in btb detection between dairy and non-dairy cattle in great britain. during the period from 2002 to 2005, 200 ...	2016	27687920
limited antibody evidence of exposure to mycobacterium bovis in feral swine (sus scrofa) in the usa.	bovine tuberculosis is a chronic disease of cattle ( bos taurus ) caused by the bacterium mycobacterium bovis . efforts have been made in the us to eradicate the disease in cattle, but spillover into wildlife and subsequent spillback have impeded progress in some states. in particular, infection in white-tailed deer ( odocoileus virginianus ) has been followed by infection in cattle in some midwestern states. infection has also been documented in feral swine ( sus scrofa ) on the hawaiian island ...	2017	27809647
isolation of mycobacterium bovis from free-ranging wildlife in south korea.	we demonstrate mycobacterium bovis infection in wild boar ( sus scrofa ) in south korea. during 2012-15, we attempted to isolate m. bovis from 847 wild animals, mainly korean water deer ( hydropotes inermis argyropus), raccoon dogs ( nyctereutes procyonoides ), and wild boar, from 11 regions in south korea. we isolated m. bovis from three of 118 wild boar (2.5%) captured in gyeonggi province, where bovine tuberculosis (btb) outbreaks have also occurred in livestock. spoligotypes and mycobacteria ...	2017	27809648
oral vaccination of cattle with heat inactivated mycobacterium bovis does not compromise bovine tb diagnostic tests.	in this study we investigated whether oral uptake of a heat inactivated m. bovis wildlife vaccine by domestic cattle induced systemic immune responses that compromised the use of tuberculin or defined antigens in diagnostic tests for bovine tb. positive skin test and blood-based ifn-γ release assay (igra) results were observed in all calves vaccinated via the parenteral route (i.e. intramuscular). in contrast, no positive responses to tuberculin or defined antigens were observed in either the sk ...	2016	27863556
genetic evaluation for bovine tuberculosis resistance in dairy cattle.	genetic evaluations for resistance to bovine tuberculosis (btb) were calculated based on british national data including individual animal tuberculin skin test results, postmortem examination (presence of btb lesions and bacteriological culture for mycobacterium bovis), animal movement and location information, production history, and pedigree records. holstein cows with identified sires in herds with btb breakdowns (new herd incidents) occurring between the years 2000 and 2014 were considered. ...	2017	27939547
vaccination of calves with mycobacterium bovis bacillus calmette-guerin reduces the frequency and severity of lesions of bovine tuberculosis under a natural transmission setting in ethiopia.	bovine tuberculosis (btb) is highly prevalent in intensive dairy farms of the urban "milk-sheds" in ethiopia, and vaccination could be a cost-effective disease control strategy. in the present study, the efficacy of bacillus calmette-guerin (bcg) to protect against btb was assessed in holstein-friesian calves in a natural transmission setting. twenty-three 2-week-old calves were subcutaneously vaccinated with bcg danish ssi strain 1331, and matched 26 calves were injected with placebo. six weeks ...	2017	28168855
epidemiological tracing of bovine tuberculosis in switzerland, multilocus variable number of tandem repeat analysis of mycobacterium bovis and mycobacterium caprae.	after 15 years of absence, in 2013 bovine tuberculosis (btb), caused by mycobacterium (m.) bovis and m. caprae, reemerged in the swiss dairy cattle population. in order to identify the sources of infection as well as the spread of the agents, molecular-epidemiologic tracing by miru-vntr analysis in combination with spoligotyping was performed. a total of 17 m. bovis and 7 m. caprae isolates were cultured from tuberculous bovine lymph nodes and analyzed with a set of 49 genetic markers by using a ...	2017	28222182
effect of mycobacterium avium subsp. paratuberculosis (map) infection on the diagnostic accuracy for mycobacterium bovis (m. bovis) infection under field conditions in cattle belonging to low m. bovis prevalence herds.	currently, the chilean authority has implemented a national eradication program for bovine tuberculosis (btb), aimed at controlling and eradicating the disease in chile. the area under study has a low within-herd prevalence, has a relatively low number of infected herds, and is one of the major milk and beef producing areas in the country. however, so far, no attempts at eradicating the disease have been successful. it has been suggested that the diagnostic tests used were either not sensitive o ...	2017	28281163
progenitor strain introduction of mycobacterium bovis at the wildlife-livestock interface can lead to clonal expansion of the disease in a single ecosystem.	mycobacterium bovis infects multiple wildlife species and domesticated cattle across south africa, and negatively impacts on livestock trade and movement of wildlife for conservation purposes. m. bovis infection was first reported in the kruger national park (knp) in south africa during the 1990s, and has since spread to infect numerous animal host species throughout the park and across south africa. whole genome sequencing data of 17 m. bovis isolates were analyzed to investigate the genomic di ...	2017	28412523
fine-mapping host genetic variation underlying outcomes to mycobacterium bovis infection in dairy cows.	susceptibility to mycobacterium bovis infection in cattle is governed in part by host genetics. however, cattle diagnosed as infected with m. bovis display varying signs of pathology. the variation in host response to infection could represent a continuum since time of exposure or distinct outcomes due to differing pathogen handling. the relationships between host genetics and variation in host response and pathological sequelae following m. bovis infection were explored by genotyping 1966 holst ...	2017	28646863