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preparation, purification, and determination of the biological activities of 12 n terminus-truncated recombinant analogues of bovine placental lactogen.removal of 13 to 15 amino acids from the n terminus of bovine placental lactogen (bpl), which according to the three-dimensional structure of pgh corresponds to a nonhelical part of bpl, did not effect its secondary structure or change the monomer content of the protein preparation. however, it remarkably decreased the binding of the prolactin (prl) type of receptors on nb2 cells with subsequent reduction in bioactivity. the binding to the growth hormone (somatogen) receptors either did not chan ...19921618771
dipstick immunoassay to detect enterohemorrhagic escherichia coli o157:h7 in retail ground beef.a sensitive and easy-to-perform dipstick immunoassay to detect escherichia coli o157:h7 in retail ground beef was developed by using a sandwich-type assay (with a polyclonal antibody to e. coli o157 as the capture antibody and a monoclonal antibody to e. coli o157:h7 as the detection antibody) on a hydrophobic polyvinylidine difluoride-based membrane. e. coli o157:h7 in ground beef could be detected within 16 h, including incubation for 12 h in enrichment broth and the immunoassay, which takes 4 ...19921622249
biochemical and topographical studies on escherichia coli cell surface. iv. giant spheroplast formation from a filamentous cell.long, nonseptate filamentous cells consisting of 5 to 40 single-cell unit lengths were formed from escherichia coli surface mutant ont-3 by treatment with a sublethal concentration of sodium dodecyl sylfate. as distinct from several other elongated cells (e.g., thymine-starved filaments), it was found here that stable giant spheroplasts, 5 to 10 micrometers in diameter, were produced by the action of lysozyme in the presence of bovine serum albumin via the gradual fusion of distinct spheroplasti ...1979374397
plasmid content and localisation of the stai (stap) gene in enterotoxigenic escherichia coli with a non-radioactive polynucleotide gene probe.enterotoxigenic escherichia coli (etec) strain p2200 of porcine origin possessed eight possibly plasmid-determined characters (k88+ raf+ hly+ col+ smr tcr su(r) sta+) and six plasmid dna bands of 4.2-93 kb. analysis of the spontaneous loss of characters and the results of matings with other e. coli strains revealed that the k88, raf, hly, smr, tcr and su(r) characters could be transferred, and that the presence of the k88 and raf characters was associated with an 83-kb plasmid. the presence and ...19921629900
opsonic activity of bovine serum and mammary secretion after escherichia coli j5 vaccination.six pairs of cows were used to determine the effects of immunization with an escherichia coli (o111:b4) j5 bacterin on in vitro opsonization of a smooth heterologous strain of e. coli. one cow in each pair was either immunized with the vaccine or sham-immunized at drying off, 30 d after drying off, and at calving. opsonizing bacteria with serum collected from vaccinated cows 21 d after calving resulted in higher mean number of intracellular bacteria per phagocytosing neutrophil than opsonizing b ...19921541744
the signal for golgi retention of bovine beta 1,4-galactosyltransferase is in the transmembrane domain.the expression and localization of bovine beta 1,4-galactosyltransferase (gal t) has been studied in mammalian cells transfected with gal t cdna constructs, and the role of the amino-terminal domains of gal t in golgi localization examined. here we demonstrate that the transmembrane (signal/anchor) domain of bovine gal t contains a positive golgi retention signal. bovine gal t was characterized in transfected cells with anti-bovine gal t antibodies, affinity-purified from a rabbit antiserum usin ...19921637374
activation of mammalian dna ligase i through phosphorylation by casein kinase ii.mammalian dna ligase i has been shown to be a phosphoprotein. dephosphorylation of purified dna ligase i causes inactivation, an effect dependent on the presence of the n-terminal region of the protein. expression of full-length human dna ligase i in escherichia coli yielded soluble but catalytically inactive enzyme whereas an n-terminally truncated form expressed activity. incubation of the full-length preparation from e. coli with purified casein kinase ii (ckii) resulted in phosphorylation of ...19921639065
meningitis in neonatal calves: 32 cases (1983-1990).case records of 32 neonatal calves with the antemortem diagnosis of meningitis were reviewed. mean age at admission was 6 days (range, 11 hours to 30 days), and the most common concurrent clinical problem was diarrhea (16/32). twenty-seven of the calves were available for necropsy. at postmortem, there was evidence of septicemia in 22 (81%) of these calves. escherichia coli was the organism most frequently isolated (11/16; 69%) from the cns. the major clinical signs of cns disturbance observed o ...19921644635
field trial to determine efficacy of an escherichia coli j5 mastitis vaccine.efficacy of an escherichia coli (o111:b4) j5 bacterin for preventing naturally occurring imi and clinical mastitis was tested in a 2.5-yr field trial in a 225-cow commercial herd. cows with odd-numbered identification were vaccinated, and cows with even-numbered identification served as unvaccinated controls for each lactation during the study. immunizations were subcutaneous on the upper part of the rib cage just posterior to the scapula at drying off, 30 d after drying off, and at calving. per ...19921541745
cdna cloning and expression of bauhinia purpurea lectin.bauhinia purpurea lectin (bpa) was purified from seeds of b. purpurea alba. the purified lectin was digested with an endoproteinase, asp-n, or trypsin and then the amino acid sequences of the resultant fragments were analyzed. furthermore, a cdna library for bpa was constructed using rna isolated from germinated bauhinia purpurea seeds. by gene cloning, the nucleotide sequence of bpa cdna and its deduced amino acid sequence were analyzed. the cloned bpa cdna comprised 1,152 nucleotides and the o ...19911657898
diverse proteins homologous to inositol monophosphatase.bovine inositol monophosphatase (imp) and several homologous proteins were found to share two sequence motifs with bovine inositol polyphosphate 1-phosphatase (ipp). these motifs may correspond to binding sites within imp and ipp for inositol phosphates or for lithium, since both substances are bound by these proteins. this suggests that the proteins homologous to imp, which have diverse biological roles but whose function is not clear, may act by enhancing the synthesis or degradation of phosph ...19911660408
expression of the phosphorylase kinase gamma subunit catalytic domain in escherichia coli.the catalytic subunit of phosphorylase b kinase (gamma) and an engineered truncated form (gamma-trc, residues 1-297) have been expressed in escherichia coli. the truncated protein included the entire catalytic domain as defined by sequence alignment with other protein kinases but lacked the putative calmodulin binding domain. full-length protein was produced in insoluble aggregates. some activity was regenerated by solubilization in urea and dilution into renaturating buffer but the activity was ...19921287663
the economic implications of bioengineered mastitis control.this paper estimates the cost of mastitis for the new york dairy sector. the average cost is found to be $125 per cow from reduced milk production, treatment, and increased culling. at the 1988 cow inventory, this translates to approximately $100 million annually for the entire dairy farm sector. when quality and production losses for the processing sector are added, the cost to the new york industry alone is nearly $150 million annually. two promising new treatments, a bacteriocin and a vaccine ...19921560148
functional reconstitution of membrane proteins in monolayer liposomes from bipolar lipids of sulfolobus acidocaldarius.membranes of sulfolobus acidocaldarius, an extreme thermophilic archaebacterium, are composed of unusual bipolar lipids. they consist of macrocyclic tetraethers with two polar heads linked by two hydrophobic c40 phytanyl chains which are thought to be arranged as a monolayer in the cytoplasmic membrane. fractionation of a total lipid-extract from s. acidocaldarius yielded a lipid fraction which forms closed and stable unilamellar liposomes in aqueous media. beef heart cytochrome c-oxidase could ...19921309769
protein phosphatase 2a is a specific protamine-kinase-inactivating phosphatase.purified preparations of a protamine protein kinase from bovine kidney cytosol [damuni, amick & sneed (1989) j. biol. chem. 264, 6412-6416] were inactivated after incubation with near-homogeneous preparations of protein phosphatase 2a1 and protein phosphatase 2a2. these protein phosphatase 2a-mediated inactivations of the protamine kinase were unaffected by highly purified preparations of inhibitor 2, but were prevented when the incubations were performed in the presence of 100 nm microcystin-lr ...19921332680
isolation and characterization of the adenylate cyclase structural gene of neurospora crassa.a single gene (nac) encoding an adenylate cyclase was cloned from the genomic dna library of neurospora crassa, using the dna fragment encoding the catalytic domain of adenylate cyclase of saccharomyces cerevisiae as a probe. the open reading frame of this gene (6900 base pairs) was interrupted three time by introns. the protein encoded consists of 2300 amino acids and has adenylate cyclase activity. n. crassa adenylate cyclase has a high degree of homology with the catalytic domains of yeast an ...19911680356
characterization of a new fimbrial antigen present in escherichia coli strains isolated from calves.thirteen escherichia coli strains isolated from calves with diarrhoea, supposed to carry a common antigen were examined for their hemagglutinating activity and compared by bacterial agglutination, double diffusion in two dimensions and by crossed immunoelectrophoresis (cie). two of the strains were examined also in the electron microscope. most of the strains agglutinated red blood cells of horse, ox, guinea pig and chicken, of which the agglutination of ox erythrocytes was mannose-resistant (mr ...19911686345
[antibody response in mice, rabbits and pigs in response to vaccination with an inactivated oil vaccine containing rotavirus and escherichia coli strains with k88, k99 and 987p fimbrial antigens].in trials with mice, rabbits and weanling piglets, four experimental charges of a combined inactivated oil vaccine against diarrhoeas in mammals were tested: the vaccine was to be implanted to sows and it contained porcine rotavirus (prv); two charges also contained bovine rotavirus and bacterins of enterotoxicogenic strains of e. coli with protective antigens k88, k99 and 987p. at low starting antibody titres the twofold i.m. implantation of 0.2 ml vaccine stimulated in mice the production of a ...19911686823
expression of bovine leukaemia virus antigens fused to ms2 polymerase in e. coli.the main core protein and segments of envelope proteins of bovine leukaemia virus fused to ms2 polymerase were expressed in e. coli. the synthesis rate varied between 3 and 25% of the total cellular proteins. blv-ms2 polymerase fusion proteins were detected immunologically using rabbit anti-blv sera, monoclonal antibodies and a serum of a blv-infected cow.19911686964
a genetic screen to identify variants of bovine pancreatic trypsin inhibitor with altered folding energetics.a genetic screening procedure has been developed to identify mutant forms of bovine pancreatic trypsin inhibitor (bpti) that can fold to an active conformation but are inactivated more rapidly than the wild-type protein. small cultures of escherichia coli containing plasmids with mutagenized bpti genes were grown in microtiter plates, lysed, and treated with dithiothreitol (dtt). under these conditions, unfolding and inactivation of the wild-type protein has a half-time of about 10 hours. varian ...19901691852
characterization of the human calmodulin-like protein expressed in escherichia coli.the protein-coding region of an intronless human calmodulin-like gene [koller, m., & strehler, e. e. (1988) febs lett. 239, 121-128] has been inserted into a pkk233-2 expression vector, and the 148-residue, m(r) = 16,800 human protein was purified to apparent homogeneity by phenyl-sepharose affinity chromatography from cultures of escherichia coli jm105 transformed with the recombinant vector. several milligrams of the purified protein were obtained from 1 l of bacterial culture. a number of pro ...19921334432
covalent binding studies on the 14c-labeled antitumor compound 2,5-bis(1-aziridinyl)-1,4-benzoquinone. involvement of semiquinone radical in binding to dna, and binding to proteins and bacterial macromolecules in situ.2,5-bis(1-aziridinyl)-1,4-benzoquinone (babq) is a compound from which several antitumour drugs are derived, such as trenimone, carboquone and diaziquone (azq). the mechanism of dna binding of babq was studied using 14c-labeled babq and is in agreement with reduction of the quinone moiety and protonation of the aziridine ring, followed by ring opening and alkylation. the one-electron reduced (semiquinone) form of babq alkylates dna more efficiently than two-electron reduced or non reduced babq. ...19901699678
cloning, expression and characterization of the human transcription elongation factor, tfiis.the cdna for the human elongation factor, tfiis, has been cloned and expressed in e. coli with the t7 expression system. this 280-amino acid tfiis protein is shorter by 21 residues than that of the mouse. the missing 21 residues are located in the amino-terminal region, which is not thought to be required for transcriptional stimulation. apart from this gap, human and mouse proteins reveal 96% overall identity and 98.5% sequence similarity if conservative substitutions are taken into account. th ...19911708494
the sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase.the cdna sequence of the flavoprotein subunit of bovine heart succinate dehydrogenase is reported. this is the first complete eukaryotic sequence of the flavoprotein subunit to be characterized, and it encodes a 665-amino acid protein that consists of a presequence and a 621-residue mature protein. the deduced bovine sequence shows homology to the corresponding peptides of prokaryotic succinate dehydrogenase and the related fumarate reductases; in particular, there is good overall homology (48%) ...19921375942
hp 0.35, a cephalosporin degradation product is a specific inhibitor of lentiviral rnases h.penicillins, cephalosporins and other betalactam antibiotics are widely used antibacterial drugs. recently it was found that some of them also have effects on proliferating eukaryotic cells (neftel, k.a. and hübscher, u. (1987) antimicrob. agents chemother. 31, 1657-1661), and one such effect was shown to be the inhibition of dna polymerase alpha (huynh do,u., neftel, k.a., spadari, s. and hübscher, u. (1987) nucl. acids res. 15, 10495-10506). the data suggested that degradation products of beta ...19911714562
effect of interferon-gamma on the production of tumor necrosis factor during acute escherichia coli mastitis.the effects of recombinant interferon-gamma on the production of tumor necrosis factor in 10 dairy cows with escherichia coli mastitis were determined. prophylactic administration of recombinant interferon-gamma prior to the experimental e. coli challenge was effective in modifying the production of endogenous tumor necrosis factor during acute stages of disease. elevated tumor necrosis factor concentrations were especially evident in cows that developed severe clinical symptoms and eventually d ...19921401365
protein engineering of the milk-clotting aspartic proteinases.calf chymosin and a fungal protease from mucor pusillus (mucor rennin) are members of the aspartic proteinases used as milk-coagulants in cheese industry. a system for production of recombinant chymosin as inclusion bodies in escherichia coli cells and its refolding into the active form was established. another expression system for production of mucor rennin in saccharomyces cerevisiae was also established. mucor rennin was efficiently excreted from the yeast host as a heavily glycosylated form ...19921455180
swine and cattle enterotoxigenic escherichia coli-mediated diarrhea. development of therapies based on inhibition of bacteria-host interactions.enterotoxigenic escherichia coli (etec) frequently occurs in diarrheal disease afflicting domestic animals. in this paper is summarized the research carried out over the last decade on the two important determinants of virulence that plays a role in the development of the infection, namely the colonizing ability of the small intestine mediated by specific fimbrial adhesins acting as lectins and the production of enterotoxins. recent progress in knowledge of the phenomenon led to alternative stra ...19911723693
bacterial expression, purification, and in vitro n-myristoylation of hiv-1 p17gag.the coding region of the n-terminal 17-kda portion of hiv-1 pr55gag (p17gag) was cloned into the pet-3c expression vector and was used to overexpress hiv-1 p17gag in escherichia coli. induction of the transformed bacteria caused the accumulation of a 17-kda polypeptide in the soluble cell fraction which was released by sonication in hypotonic nondetergent buffer. the 17-kda polypeptide was purified by ammonium sulfate precipitation and successive chromatography on g-75 sephadex, deae-sephacel, a ...19921458053
cloning, sequencing, expression, and functional studies of a 15,000-molecular-weight haemophilus somnus antigen similar to escherichia coli ribosomal protein s9.haemophilus somnus is a gram-negative bacterium capable of causing a number of disease syndromes in cattle. this article describes the cloning and characterization of a gene coding for a 15,000-molecular-weight (15k) polypeptide which reacts strongly with antiserum against h. somnus. analysis of plasmid-encoded polypeptides by polyacrylamide gel electrophoresis showed that the corresponding gene is the second in a transcriptional unit. the first gene codes for a protein with a molecular weight o ...19921729207
susceptibility of escherichia coli isolated from intramammary infections to phagocytosis by bovine neutrophils.thirteen escherichia coli isolated from naturally occurring imi were tested for susceptibility to phagocytosis by bovine blood neutrophils. isolates were opsonized in pooled serum collected from nine healthy lactating cows. bacteria isolated from imi first diagnosed within 3 d after calving were more resistant to phagocytosis than were isolates from imi originating during either the first half of the dry period or later during lactation. duration of the imi was negatively correlated with both ph ...19921474201
determination of the carbohydrate-binding site of bauhinia purpurea lectin by affinity chromatography.to determine the carbohydrate-binding site of bauhinia purpurea lectin (bpa), a d-galactose- and lactose-binding lectin, a peptide which interacts with lactose was purified from endoproteinase asp-n digests of bpa by chromatography on a lactose-sepharose column. it consists of nine amino acids and its amino acid sequence is asp-thr-trp-pro-asn-thr-glu-trp-ser. a tryptic fragment with the ability to interact with lactose was also purified and found to contain this sequence, consisting of nine ami ...19921517321
stimulation of dna polymerase alpha activity by microtubule-associated proteins.microtubule-associated protein 2 (map2) isolated from porcine brains stimulated the activity of dna polymerase alpha immunopurified from calf thymus or human lymphoma cells, in a dose-dependent manner. this stimulation was pronounced when activated dna or poly(da).(dt)10 was used as the template-primer. dna polymerase alpha bound to a map2-immobilized column, whereas preincubation of the enzyme with unbound map2 prevented binding to the column. these events suggested that a physical binding occu ...19911742279
[synthesis of the e-antigen of the hepatitis b virus (hbeag) in eukaryotic cells by a recombinant strain of the vaccinia virus].the recombinant plasmids containing the gene for hepatitis b viral core-antigen with the pre-core-sequence controlled by the early-late promoter of the 7.5' k protein gene were constructed. the recombinant strains of vaccinia virus were obtained on their basis (vhbe42-1 and vhbe42-3) selectively expressing hbeag of hepatitis b virus. the kinetics of hbeag synthesis was studied in infected cells as well as secretion of the protein into culturing medium. three proteins were found by blotting techn ...19911745274
phospholipase a2 engineering. x-ray structural and functional evidence for the interaction of lysine-56 with substrates.site-directed mutagenesis studies of bovine pancreatic phospholipase a2 (pla2, overproduced in escherichia coli) showed that replacement of surface residue lys-56 by a neutral or hydrophobic amino acid residue resulted in an unexpected and significant change in the function of the enzyme. the kcat for phosphatidylcholine micelles increases 3-4-fold for k56m, k56i, and k56f and ca. 2-fold for k56n and k56t but does not change for k56r. these results suggest that the side chain of residue 56 has s ...19911751497
potent bactericidal activity of bovine lactoferrin hydrolysate produced by heat treatment at acidic ph.a hydrolysate of bovine lactoferrin produced by heat treatment under acidic conditions had antibacterial activity at concentrations of 10 micrograms/ml in the culture medium. the optimal degree of hydrolysis for this activity was about 10%. heat-treated lactoferrin, treated at ph 2.0 and 120 degrees c for 15 min and degree of hydrolysis of about 10%, had no fe-binding capacity (0%) and less antigenicity (about 10(-6) than untreated lactoferrin. heat-treated lactoferrin increased in antibacterial ...19911757616
rapid procedure for detecting enterohemorrhagic escherichia coli o157:h7 in food.a sensitive, specific procedure was developed for detecting escherichia coli o157:h7 in food in less than 20 h. the procedure involves enrichment of 25 g of food in 225 ml of a selective enrichment medium for 16 to 18 h at 37 degrees c with agitation (150 rpm). the enrichment culture is applied to a sandwich enzyme-linked immunosorbent assay (elisa) with a polyclonal antibody specific for e. coli o157 antigen as the capture antibody and a monoclonal antibody specific for enterohemorrhagic e. col ...19911768144
protective effect of tissue ferritins in experimental escherichia coli infection of mice in vivo.the effect of ferritins from horse (fh) and bovine (fb) spleen and murine liver (fm) on the survival rate of cfw mice lethally infected with escherichia coli (strain 8440-78 k 80/b) was evaluated. ferritins given intravenously 24 h before intravenous inoculation of bacteria, protected mice most effectively from death due to infection. the effect was dose dependent. at 500 micrograms of ferritin per mouse, the maximum survival rates were 86% (fh), 81% (fm) and 79% (fb), while only 5% of the contr ...19911768608
prevalence of antibiotic-resistant escherichia coli in danish pigs and cattle.the present paper reports on the antibiotic resistance of e. coli isolated from danish piglets and calves in 1987-1988, and compares the results with similar investigations performed during the periods 1971-1972 and 1977-1978. rectal swabs from 52 piglets and from 78 calves were examined. all the animals studied harboured resistant e. coli. this is a significant increase compared to the previously conducted investigations. the number of strains having three or more resistance markers did not dif ...19911772647
virulence factors associated with cytotoxic necrotizing factor type two in bovine diarrheic and septicemic strains of escherichia coli.forty-three bovine isolates of escherichia coli producing a second type of cytotoxic necrotizing factor (cnf2) and three k-12 strains carrying different vir plasmids coding for cnf2 were tested for the presence of several virulence factors. most of the strains were serum resistant (79%), produced an aerobactin (70%), and adhered to calf villi (53%); some of them produced a colicin (32%) and a hemolysin (9%). these strains were also tested by a colony hybridization assay with gene probes for six ...19911774259
the antibacterial peptide seminal plasmin alters permeability of the inner membrane of e. coli.seminal plasmin (spln) a 47-residue peptide, isolated from bovine seminal plasma, exhibits antibacterial activity against gram-positive and gram-negative bacteria. although spln strongly inhibits the transcription of various natural and synthetic templates by e. coli rna polymerase in vitro, it also associates with model membranes of phosphatidylcholine and phosphatidic acid. we have undertaken experiments to ascertain whether spln permeabilizes the bacterial inner membrane and thereby exerts it ...19921535050
dietary selenium effects on milk eicosanoid concentration in dairy cows during coliform mastitis.the effect of selenium deficiency on the product profile of arachidonic acid oxidation by enzymatic pathways in holstein cows with experimentally-induced coliform mastitis was investigated. the animals were fed dairy rations containing 0.05 mg se/kg dry matter, with the supplemented group receiving additional se to increase the dietary concentration to approximately 0.35 mg se/kg dry matter. cows were inoculated intracisternally with 30 colony-forming-units of escherichia coli at 14-16 weeks of ...19911665569
an f41-k88-related genetic determinant of bovine septicemic escherichia coli mediates expression of cs31a fimbriae and adherence to epithelial cells.a genetic determinant related to that encoding the f41 fimbrial adhesin was cloned from a bovine septicemic isolate of escherichia coli. this determinant was found to mediate expression of morphologically distinct fimbriae in e. coli hb101. the gene encoding the fimbrial subunit protein was identified, and the nucleotide sequence was determined. homology with the amino-terminal amino acid sequence of cs31a (j. girardeau, m. der vartanian, j. ollier, and m. contrepois, infect. immun. 56:2180-2188 ...19911675627
in vitro studies on mild alkali induced lesions in dna.s1 nuclease hydrolysis, benzoylated naphthoylated deae cellulose (bnd-cellulose) chromatography as well as certain immunological and genetic techniques have been used to evaluate the effect of mild alkali (ph10.0) on the dna molecule. native calf thymus dna after exposure to alkaline ph10.0 when subjected to s1 nuclease hydrolysis released significant amount of acid soluble nucleotides as compared to the untreated control. with pbr322 dna, the population of linear dna species increased on s1 dig ...19911789792
expression of bovine inhibin beta subunit in escherichia coli.1. a dna fragment encoding the beta subunit of bovine inhibin was amplified using the polymerase chain reaction and was cloned in plasmids puc8 and pur291. 2. cultures of escherichia coli tg2 harbouring pkdk37, a pur291-derived recombinant plasmid, produced a novel protein with a molecular weight of 130,000 corresponding to a beta-galactosidase-inhibin beta fusion protein. 3. the fusion protein was purified from inclusion bodies by solubilization in 8 m urea followed by an ion-exchange and gel p ...19911794452
comparative immunochemical analysis of the antigenic structures of the dna-binding histone-like hu protein from escherichia coli and eukaryotic histones.the general antigenic structures of the dna-binding hu protein from e. coli, histones h1, h2a, h2b, h3, and h4 from calf thymus, and histone h5 from chick erythrocytes were compared in immunoassays with the aid of monospecific polyclonal antibodies to the hu protein and the individual histones. a partial cross-reaction between the hu protein and antibodies to histones h1 and h5 was demonstrated. the reaction titres obtained in a solid-phase enzyme immunoassay indicated that the cross-reactions w ...19911797140
[detection of verotoxin-producing escherichia coli using polymerase chain reaction from dairy cattle].the vero cytotoxin (vt) is responsible for hemorrhagic colitis and hemolytic uremic syndrome. polymerase chain reaction (pcr) was used to detect vt-producing coliform bacteria from dairy cattle. it was found that 39 (33.3%) of the 117 fecal samples examined were recognized with vt genes in bglb enrichment broth by the pcr method (named bglb-pcr). of the vt-positive samples, 31 samples (26.5%) were found to have vt-producing escherichia coli. frequencies of isolation in younger cattle (under 5 mo ...19921293218
impact of transfer from animal-source insulins to biosynthetic human insulin (rdna e coli) in patients with diabetes mellitus.six hundred forty-eight patients (50.5% men; 49.5% women) with diabetes mellitus on animal-source insulin therapy for at least five years were studied. in this patient population, approximately 68.7% had type i insulin-dependent diabetes mellitus and 31.3% had type ii noninsulin-dependent diabetes mellitus, nonetheless requiring insulin therapy. patients were voluntarily transferred from animal-source insulin to biosynthetic human insulin derived by recombinant dna technology from genetically al ...19911799920
[the immunogenicity of mono- and associated vaccines made from strains of opportunistic bacteria].in experiments on white mice the immunogenic potency of two variants of combined vaccines prepared from strains of opportunistic bacteria was compared with that of monovaccines used as the components of the combined vaccines. all the vaccines under study were found highly immunogenic. decrease of monovaccine share in the combined vaccine did not reduce the immunogenic potency to any of the strains. an 8- to 16-fold increase in the level of specific antibodies in the colostric lactosera of cows i ...19911801485
pathogenic escherichia coli o157:h7 and their detection.enterohaemorrhagic escherichia coli o157:h7 is of major concern to the food industry due to high pathogenicity of this foodborne organism. for the detection of these bacteria a special agar medium with a fluorogenic substrate has been developed. the medium uses the characteristics of this e. coli serotype not to ferment sorbitol and not to produce beta-glucuronidase. in contrast, approximately 96% of all other strains of e. coli are sorbitol-positive and nearly all of them are beta-glucuronidase ...19911817428
development of genomic probes to sarcocystis cruzi (apicomplexa).a genomic library of sarcocystis cruzi sporozoite dna was constructed in bacteriophage lambda gt10. recombinant phages containing insert dna were selected by growth on escherichia coli strain c600 hfla150. of 14 clones examined, 11 contained dna inserts ranging in size from approximately 1.45 kilobase (kb) to 6.18 kb. insert dna from four of these clones specifically hybridized to 32p-labelled s. cruzi merozoite dna. one of these insert dna, clone sl41, was selected and labelled with 32p. this p ...19911837193
simian virus 40 vp2/3 small structural proteins harbor their own nuclear transport signal.we have used a microinjection approach to identify a domain of the simian virus 40 (sv40) structural proteins vp2 and vp3(vp2/3) responsible for their nuclear transport. by using both synthetic peptides, containing small regions of vp2/3 conjugated to bovine serum albumin (bsa), and beta-galactosidase-vp3 fusion proteins, we have narrowed this nuclear transport signal (nts) to 9 amino acids (198 to 206 of vp3 or 316 to 324 of vp2), gly-pro-asn-lys-lys-lys-arg-lys-leu. the porter proteins carryin ...19911847270
distribution and characteristics of verocytotoxigenic escherichia coli isolated from ontario dairy cattle.faecal swabs obtained from a random sample of 1131 cows and 659 calves on 100 southern ontario dairy farms were examined for verocytotoxigenic escherichia coli (vtec) using a vero cell assay. five isolates from each positive culture were tested similarly. positive colonies were examined with dna probes for shiga-like toxin i (slt-i) and slt-ii sequences. probe-negative colonies were tested for neutralization of verocytotoxicity using anti-slt-i and anti-slt-iiv antisera. colonies showing no neut ...19921601076
a new diarrhoeic syndrome with ataxia in young charolais calves: clinical and microbiological studies.a new diarrhoeic syndrome was examined clinically in 19 one to two-week old charolais calves. it differs from other digestive disorders in calves of this age in the discrete diarrhoeic signs, the absence of dehydration and the presence of signs of ataxia. the microbiological study carried out for three consecutive years in 58 sick calves and nine healthy control calves demonstrated the special role of e coli possessing virulence markers from septicaemic strains (cs31a, col v). the clinical signs ...19911853535
escherichia coli o157:h7 and its significance in foods.escherichia coli o157:h7 was conclusively identified as a pathogen in 1982 following its association with two food-related outbreaks of an unusual gastrointestinal illness. the organism is now recognized as an important cause of foodborne disease, with outbreaks reported in the u.s.a., canada, and the united kingdom. illness is generally quite severe, and can include three different syndromes, i.e., hemorrhagic colitis, hemolytic uremic syndrome, and thrombotic thrombocytopenic purpura. most out ...19911854598
dna hybridization and latex agglutination for detection of heat-labile- and shiga-like toxin-producing escherichia coli in meat.dna-hybridization and latex-agglutination tests were used for screening of a group of escherichia coli isolates for heat-labile enterotoxin (lt)- and shiga-like toxin (slt1 or vt1) -producing strains, respectively. strains tested originated from 162 meat samples (poultry, pigs and beef) chosen at random. additionally lt- and slt1-producing reference strains were tested. the dna-hybridization technique allowed screening of large numbers of strains, whereas large scale testing of strains by latex ...19911863526
antibodies to iron-regulated outer membrane proteins of coliform bacteria isolated from bovine intramammary infections.expression of iron-regulated outer membrane proteins (omp) by escherichia coli and klebsiella pneumoniae initially isolated from bovine intramammary infections (imi) was investigated. additionally, the presence of antibodies in bovine serum and mammary secretion directed against the iron-regulated omp was examined. outer membrane proteins were separated by sodium-dodecyl polyacrylamide electrophoresis. detection of immunoglobulin g directed against omp was by immunoblotting. all gram-negative ba ...19911866891
cryptosporidiosis in neonatal calves: 277 cases (1986-1987).from january 1986 through december 1987, 277 cases of cryptosporidiosis in calves were diagnosed by the south dakota state university diagnostic laboratory. cryptosporidium sp was the only pathogen identified in 142 (51.3%) of the calves. concurrent infections with rotavirus, coronavirus, escherichia coli, salmonella sp, bovine viral diarrhea virus, or other pathogens were identified in the remaining 135 calves. after elimination of cases involving autolyzed specimens or calves with chronic diar ...19911874678
n-acetyl-beta-d-glucosaminidase, etiologic agent, and duration of clinical signs for sequential episodes of chronic clinical mastitis in dairy cows.this study examined effects of repeated episodes of clinical mastitis in chronically infected quarters on milk n-acetyl-beta-d-glucosaminidase activity and duration of clinical signs. milk samples were collected at each clinical onset from 49 chronic mastitis cases on a 1700-cow michigan dairy farm. there were 49 first episodes of clinical mastitis, 49 second episodes, and 13 episodes of third or more. agents isolated were staphylococcus aureus (18.4%), staphylococcus (7.3%), no growth (20.2%), ...19911880262
use of probes to detect virulence factor dna sequences in escherichia coli strains isolated from foods.escherichia coli strains were isolated from 96 food samples (32 milks, 4 dairy products, 36 raw meats, 7 meat products, 7 sandwiches and 10 ready-to-eat meals). a total of 306 colonies was submitted to hybridization assays with dna probes for the following virulence factors: heat-labile toxins (lt-i and lt-ii), heat-stable toxins (st-h and st-p). shiga-like toxins (slt-i and slt-ii), adherence factor of enteropathogenic e. coli (eaf) and invasive factor (inv). six colonies isolated from 4 food s ...19911677256
clonal relationships among escherichia coli serogroup 06 isolates from human and animal infections.the clonal relationship of thirty e. coli strains of 0 antigen serotype 06 isolated from human, dog, pig or cow infections were investigated. two main clones with serotypes 06 : h1 or 06 : h31, h- were identified. isolates from humans, dogs, pigs and cows were found in both clones, indicating that animals are a possible source for human extraintestinal escherichia coli strains. two human etec (06 : h16) and two pig isolates (06 : h10) were not related to the 06 : h1 or 06 : h31, h- e. coli clone ...19911884980
the effect of cyclosporin a on cationized bovine serum albumin-induced nephropathy in nzw rabbits.the effect of cyclosporin a (cya) was studied on the morphology and protein excretion of a rabbit chronic serum sickness nephritis using cationized bovine serum albumin (cbsa). one group of rabbits was given intravenous (i.v.) immunizing doses of cbsa and escherichia coli endotoxin. one week later, these animals began a 6-week i.v. injection schedule of cbsa only. a second group followed the same injection protocol, but was given intramuscular (i.m.) cya for 3 days prior to the immunizing dose o ...19921625057
the effect of lipopolysaccharide on bovine mammary macrophage function.the effect of escherichia coli lipopolysaccharide (lps) on the expression of major histocompatibility complex (mhc) class ii molecules by bovine mammary macrophages was examined. the ability of lps-treated mammary macrophages to support antigen-specific t-cell proliferation, as a measure of their antigen presentation ability, was also evaluated. for this purpose, control and lps-treated macrophages were pulsed with heat-killed staphylococcus aureus and then cultured with s. aureus-sensitized t-c ...19911889031
sensitive immunoassay for rat parvalbumin: tissue distribution and developmental changes.a sensitive enzyme immunoassay for measurements of rat parvalbumin was established using antibodies raised in rabbits with parvalbumin purified from skeletal muscles. antibodies in the antiserum were purified with a parvalbumin-coupled sepharose column. the sandwich-type immunoassay system for parvalbumin was composed of polystyrene balls with immobilized purified antibodies and the same antibodies labeled with beta-d-galactosidase from escherichia coli. the assay was highly sensitive and the mi ...19911892868
study of mammalian selenocysteyl-trna synthesis with [75se]hse.the mechanisms of the synthesis of mammalian selenocysteyl-(scy)-trna were studied using [75se]h2se. h2se was prepared from [75se]selenite, glutathione, nadph and glutathione reductase, and was purified by chromatography. it was confirmed that this h2se was a se donor in the reaction of the synthesis of scy-trna. [75se]scy, liberated from aminoacyl-trna, was analyzed by tlc on silica gel an subsequent autoradiography. the activity of scy-trna synthesis was found in the supernatant at 105,000 x g ...19911894009
[coliform bacilli which produce cytotoxins: importance in veterinary medicine and public health].this article reviews published data (october 1st, 1990) on 4 types of cytotoxins produced by animal and human pathogenic strains of e coli, ie, verotoxins, also named shiga-like toxins, cytotoxic necrotizing factors, haemolysin, and cytolethal distending toxin. the biological and molecular properties are described for each type of cytotoxin, the association of producing strains with animal pathology, and the role of domestic animals as reservoirs of strains pathogenic for man.19911897865
analyzing the substrate specificity of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase by co-expressing it with mammalian g protein alpha subunits in escherichia coli.a dual plasmid system was used to examine the protein and acyl-coa specificities of saccharomyces cerevisiae myristoyl-coa:protein n-myristoyltransferase (nmt) by co-expressing it in escherichia coli with each of four homologous alpha subunits of the signal-transducing, heterotrimeric g proteins. exogenous [3h]myristate was incorporated into rat gi alpha 1 and rat go alpha but not into bovine gs alpha or human gz alpha. oxygen for methylene group substitutions in myristate result in analogs with ...19911903791
antigenic homology among gram-negative organisms isolated from cattle with clinical mastitis.this study examined the degree of serologic homology among mastitis pathogens. antibodies were raised against the rc mutant, escherichia coli o111:b4 (strain j5) and affinity purified against lipopolysaccharide derived from the ra mutant, salmonella typhimurium tv119. these antibodies reacted with a battery of unrelated gram-negative bacteria in whole cell elisa. bacteria with strong cross-reactions included a heterologous, smooth e. coli, salmonella dublin, s. typhimurium, salmonella newport, a ...19911907303
controlling acute escherichia coli mastitis during the periparturient period with recombinant bovine interferon gamma.the efficacy of recombinant bovine interferon (rboifn)-gamma against experimentally induced escherichia coli mastitis during the periparturient period was investigated. dairy cows intramammarily treated with rboifn-gamma 24 h before the e. coli challenge had fewer infected quarters, lower clinical scores, and infections of shorter duration when compared to placebo-treated animals. all rboifn-gamma treated cows survived the experimental e. coli challenge. however, placebo treated cows had a 42% m ...19911908159
effect of site-directed mutagenic alterations on adp-ribosyltransferase activity of the a subunit of escherichia coli heat-labile enterotoxin.previous studies of the s1 subunit of pertussis toxin, an nad(+)-dependent adp-ribosyltransferase, suggested that a small amino-terminal region of amino acid sequence similarity to the active fragments of both cholera toxin and escherichia coli heat-labile enterotoxin represents a region containing critical active-site residues that might be involved in the binding of the substrate nad+. other studies of two other bacterial toxins possessing adp-ribosyltransferase activity, diphtheria toxin and ...19911908825
high cortisol concentrations and mediation of the hypogalactia during endotoxin-induced mastitis.a series of experiments was conducted to define the role of cortisol in the hypogalactia during endotoxin-induced mastitis. in the first experiment, three of six nonmastitic cows were given a continuous infusion of trilostane, a 3 beta-hydroxysteroid dehydrogenase inhibitor that blocks enhanced cortisol synthesis. trilostane had no effect in these cows. in the second experiment, six midlactation cows were given 10 micrograms of endotoxin in each of two homolateral quarters to induce mastitis. th ...19921569265
partial budget analysis of vaccinating dairy cattle against coliform mastitis with an escherichia coli j5 vaccine.partial budget analysis of clinical coliform mastitis prevention supported vaccination of dairy cows with an escherichia coli j5 vaccine. increased profits of $57/cow lactation were predicted using a computer spreadsheet derived partial budget with generalized herd input data. herd vaccination programs were predicted to be profitable when greater than 1% of cow lactations resulted in clinical coliform mastitis. herd vaccination programs were predicted to be profitable at all herd milk production ...19911917656
structural elements required for the cooperative binding of the herpes simplex virus origin binding protein to oris reside in the n-terminal part of the protein.the origin binding protein (obp) of herpes simplex virus type 1 is required to activate a viral origin of replication in vivo. we have used intact obp as well as a truncated form of the protein expressed in escherichia coli to investigate the protein-protein interactions, as well as the protein-dna interactions involved in the formation of a nucleoprotein complex at a viral origin of replication (oris) in vitro. the salient findings demonstrate that the n-terminal part of obp is required for the ...19921324937
immunoreactive alpha a crystallin in rat non-lenticular tissues detected with a sensitive immunoassay method.for the quantitative analysis of the a subunit of alpha crystallin (alpha a) in the lens and for the survey of possible existence of alpha a in the non-lenticular tissues, we have established a highly sensitive and specific immunoassay method for alpha a. antisera to alpha a were raised in rabbits with alpha a purified from bovine lens, or the c-terminal decapeptide (eekpssapss) of alpha a (alpha apep). the antibodies to alpha a and alpha apep were purified by the use of an alpha a-coupled sepha ...19911932094
denaturant-dependent folding of bovine pancreatic trypsin inhibitor mutants with two intact disulfide bonds.the equilibrium and kinetic behavior of the guanidine hydrochloride (gdn-hcl) induced unfolding/refolding of four bovine pancreatic trypsin inhibitor (bpti) mutants was examined by using ultraviolet difference spectroscopy. in three of the mutants, we replaced the buried 30-51 disulfide bond with alanine at position 51 and valine (val30/ala51), alanine (ala30/ala51), or threonine (thr30/ala51) at position 30. for the fourth mutant, the solvent-exposed 14-38 disulfide was substituted by a pair of ...19901693524
cdna and deduced amino acid sequence of a mouse dna repair enzyme (apex nuclease) with significant homology to escherichia coli exonuclease iii.we purified a mouse dna repair enzyme having apurinic/apyrimidinic endonuclease, dna 3'-phosphatase, 3'-5'-exonuclease and dna 3' repair diesterase activities, and designated the enzyme as apex nuclease. a cdna clone for the enzyme was isolated from a mouse spleen cdna library using probes of degenerate oligonucleotides deduced from the n-terminal amino acid sequence of the enzyme. the complete nucleotide sequence of the cdna (1.3 kilobases) was determined. northern hybridization using this cdna ...19911939131
isolation and characterization of a saccharomyces cerevisiae mutant disrupted for the succinate dehydrogenase flavoprotein subunit.a partial genomic clone of the flavoprotein subunit of the mitochondrial enzyme, succinate dehydrogenase (ec 1.3.99.1) from saccharomyces cerevisiae has been isolated. the partial clone was used to construct, by targeted gene disruption, a yeast mutant with a defective flavoprotein subunit gene. submitochondrial membranes from the mutant are defective in activities requiring a functional succinate dehydrogenase but not in other respiratory chain activities. in addition, the mutant contains signi ...19911939170
identification of enteropathogenic escherichia coli isolated in britain as enteroaggregative or as members of a subclass of attaching-and-effacing e. coli not hybridising with the epec adherence-factor probe.strains of escherichia coli from sporadic cases of diarrhoea and belonging to serotypes o44:h18, o55:h7, o111ab:h21, o111ab:h25 or o126:h27 were examined for virulence properties. with the exception of o111ab:h25 these are considered to be classical enteropathogenic e. coli (epec) serotypes. the strains had been isolated in britain from the faeces of children less than 3 years old. of the serotypes examined, 7 of 13 o44:h18 strains, all of 10 o111ab:h21 strains and 13 of 21 o126:h27 strains belo ...19911941998
intramammary administration of gentamicin as treatment for experimentally induced escherichia coli mastitis in cows.in 8 holstein cows, 50 colony-forming units (cfu) of escherichia coli was administered into 1 mammary gland. infections were established in all inoculated glands. in 4 of the 8 cows, 500 mg of gentamicin sulfate was administered by intramammary infusion 14 hours after inoculation; the other 4 cows were untreated controls. infusions of gentamicin also were given after each of the 3 successive milkings after the initial infusion, so that a total dose of 2 g of gentamicin was given to each of the t ...19921595964
molecular cloning and expression of biologically active human glia maturation factor-beta.glia maturation factor-beta, a protein found in the brains of all vertebrates thus far examined, appears to play a role in the differentiation, maintenance, and regeneration of the nervous system. using oligonucleotide probes based on the sequences of three tryptic peptides derived from bovine glia maturation factor-beta, we screened a human brainstem cdna library in lambda gt11. a 0.7-kb clone was isolated, sequenced in its entirety, and found to encode a polypeptide of 142 amino acids which co ...19911712830
cloning and sequence analysis of the human liver rhodanese: comparison with the bovine and chicken enzymes.the cdna for the human rhodanese (thiosulfate: cyanide sulfurtransferase, ec 2.8.1.1), a nuclearly encoded protein of the mitochondrial matrix, was isolated from a human fetal liver cdna library. nucleotide sequence revealed an open reading frame coding for a polypeptide of 295 amino acids, which presented a 57% and 58% identity with the bovine and avian rhodanese, respectively. the analysis of the 5'-ends of the coding region gave no evidence for the presence of a cleavable signal sequence as f ...19911953758
mammalian mitochondrial chaperonin 60 functions as a single toroidal ring.chaperonins are thought to participate in the process of protein folding in bacteria and in eukaryotic mitochondria and chloroplasts. while some chaperonins are relatively well characterized, the structures of the mammalian chaperonins are unknown. we have expressed a mammalian mitochondrial chaperonin 60 in escherichia coli and purified the recombinant protein to homogeneity. structural and biochemical analyses of this protein establish a single toroidal structure of seven subunits, in contrast ...19921346131
mapping of sequential epitopes recognized by monoclonal antibodies on the bovine leukaemia virus external glycoproteins expressed in escherichia coli by means of antipeptide antibodies.a lambda gt11 cdna library prepared from bovine leukaemia virus (blv)-producing ovine cells was screened with a cocktail of anti-blv gp51 monoclonal antibodies (mabs). four recombinant phages with inserts of about 2-5 kbp were isolated. one, lambda blv-gp51-1, was sequenced and shown to encode the c-terminal part of gp51 and all of gp30. this insert was subcloned into pev-vrf1 and expressed in escherichia coli n-4830-1 cells. the blv product and a series of antipeptide antibodies were used to lo ...19921383413
synthesis of bovine leukemia virus antigens in escherichia coli.plasmids were constructed by the use of pex vectors that encode and express different parts of the bovine leukemia virus (blv): main core protein p24, nucleic acid-binding protein p12, transmembrane protein gp30, and different segments of envelope protein gp51. expression of fusion proteins with molecular weights higher than 117 kd for all recombinant plasmids was shown in coomassie-blue stained gels and by western blot analysis with rabbit anti-blv sera. coupling of a gp51-encoding with a p24-e ...19901966363
functional interactions of stimulatory and inhibitory gdp/gtp exchange proteins and their common substrate small gtp-binding protein.smg gds and rho gdi are stimulatory and inhibitory gdp/gtp exchange proteins, respectively, for a group of ras p21-related small gtp-binding proteins (g proteins). rho p21 is a common substrate small g protein for both gdp/gtp exchange proteins. we examined here the functional interactions of these gdp/gtp exchange proteins with rho p21 as a substrate. smg gds and rho gdi interacted with the gdp-bound form of rho p21 and thereby stimulated and inhibited, respectively, the dissociation of gdp. th ...19921634508
the heat-shock protein clpb in escherichia coli is a protein-activated atpase.the clpb gene in escherichia coli encodes a heat-shock protein that is a close homolog of the clpa gene product. the latter is the atpase subunit of the multimeric atp-dependent protease ti (clp) in e. coli, which also contains the 21-kda proteolytic subunit (clpp). the clpb gene product has been purified to near homogeneity by deae-sepharose and heparin-agarose column chromatographies. the purified clpb consists of a major 93-kda protein and a minor 79-kda polypeptide as analyzed by polyacrylam ...19921400361
rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases.a gene from rhizobium meliloti coding for an adenylate cyclase was sequenced, and the deduced protein sequence was compared with those of other known adenylate cyclases. no similarity could be detected with the procaryotic counterparts. however, striking similarity was found with the catalytic region of saccharomyces cerevisiae adenylate cyclase, the cytoplasmic domains of bovine adenylate cyclase, and two mammalian guanylate cyclases. the gene was fused to the enteric beta-galactosidase, and th ...19901970565
factors influencing the adherence of strains of streptococcus bovis and escherichia coli isolated from ruminal epithelium.two strains of streptococcus bovis (a1 and a5) and one strain of escherichia coli (0141:h28) isolated from the surface of bovine ruminal mucous epithelium were examined for adherence to isolated and cultured ruminal epithelial cells. the e. coli adhered to the target cell by means of fimbriae, which had several common properties with type 1 common fimbriae and caused mannose-sensitive haemagglutination. the a1 strain of s. bovis was devoid of fimbriae and its adherence to the epithelial surface ...19901974372
identification of a groes-like chaperonin in mitochondria that facilitates protein folding.mitochondria contain a polypeptide that is functionally equivalent to escherichia coli chaperonin 10 (cpn10; also known as groes). this mitochondrial cpn10 has been identified in beef and rat liver and is able to replace bacterial cpn10 in the chaperonin-dependent reconstitution of chemically denatured ribulose-1,5-bisphosphate carboxylase. thus, like the bacterial homologue, mitochondrial cpn10 facilitates a k(+)- and mg.atp-dependent discharge of unfolded (or partially folded) ribulose bisphos ...19901977163
nine dna probes for detection of toxin and adhesin genes in escherichia coli isolated from diarrhoeal disease in animals.dna gene probes specific for genes encoding heat labile enterotoxin (lti), heat stable enterotoxins (stia, stii), vero cytotoxins (vt1, vt2), and adhesins k88 (f4), k99(f5), f41 and 987p(f6) were used to examine 873 isolates of e. coli from cases of diarrhoea (680 from pigs, 187 from cattle and six from sheep). a total of 188 were toxin gene positive and of these 84 belonged to the classical etec serogroups. of the other 104 toxin gene positive strains, 80 hybridized with the vt2 probe of which ...19901978952
glycosylation of insulin-like growth factor binding protein-3 (igfbp-3) is not required for potentiation of igf-i action: evidence for processing of cell-bound igfbp-3.insulin-like growth factor binding protein-3 (igfbp-3) is unique among the igf binding proteins in its extensive glycosylation in the native state. to determine the functional significance of carbohydrate moieties on igfbp-3, we examined the effects of nonglycosylated escherichia coli-derived recombinant human igfbp-3 (higfbp-3e. coli) and glycosylated chinese hamster ovary cell-derived higfbp-3 (higfbp-3cho) on igf-i action in cultured bovine fibroblasts. both higfbp-3 preparations bound igf-i ...19911720092
characterization of bovine septicemic, bovine diarrheal, and human enteroinvasive escherichia coli that hybridize with k88 and f41 accessory gene probes but do not express these adhesins.certain dna probes derived from accessory genes of cloned k88 and f41 determinants hybridize with escherichia coli strains that express k88 or f41 and with certain other e. coli strains that do not express these antigens. we found that these probes hybridized with human enteroinvasive e. coli, and with bovine e. coli isolates which produced a fatal septicemia in experimentally infected piglets. these strains did not hybridize with probes derived from the structural subunit genes encoding the k88 ...19901980001
immune complex induced experimental colitis: beneficial effect of sulphasalazine in rabbits.experimental colitis was induced in rabbits by exposing the colon mucosa to 1% formalin followed by i.v. injections of soluble immune complexes made with antigen in excess. the animals were preimmunized with escherichia coli o14:k7:h--inducing antibodies cross-reactive to intestinal epithelium. animals with this colitis were divided in two groups. one group was treated with sulphasalazine and the other was given vehicle only. sulphasalazine was administered daily at 125.5 mumol (50 mg) per kg bo ...19901983323
pseudomonas cepacia adherence to respiratory epithelial cells is enhanced by pseudomonas aeruginosa.pseudomonas aeruginosa and pseudomonas cepacia are both opportunistic pathogens of patients with cystic fibrosis. the binding characteristics of these two species were compared to determine if they use similar mechanisms to adhere to respiratory epithelial cells. p. cepacia 249 was shown to be piliated, but there was no detectable homology between p. aeruginosa pilin gene probes and p. cepacia genomic dna. p. cepacia and p. aeruginosa did not appear to compete for epithelial receptors. in the pr ...19901983811
16s ribosomal dna amplification for phylogenetic study.a set of oligonucleotide primers capable of initiating enzymatic amplification (polymerase chain reaction) on a phylogenetically and taxonomically wide range of bacteria is described along with methods for their use and examples. one pair of primers is capable of amplifying nearly full-length 16s ribosomal dna (rdna) from many bacterial genera; the additional primers are useful for various exceptional sequences. methods for purification of amplified material, direct sequencing, cloning, sequenci ...19911987160
a bovine papillomavirus e1-related protein binds specifically to bovine papillomavirus dna.the e1 open reading frame of bovine papillomavirus (bpv) was expressed as a reca-e1 fusion protein in escherichia coli. the bacterially expressed reca-e1 protein exhibited sequence-specific dna binding activity; strong binding to the region from nucleotides 7819 to 93 on the bpv genome (designated region a) and weak binding to the adjacent region from nucleotides 7457 to 7818 (region b) were observed. the interaction between the bpv-derived reca-e1 protein and region a appeared to be highly spec ...19911654443
modulation of function of bovine polymorphonuclear leukocytes and lymphocytes by high temperature in vitro and in vivo.function of polymorphonuclear leukocytes (pmnl) and proliferation of lymphocytes after stimulation with mitogens were evaluated in vitro at incubation temperatures of 38.5 and 42 c, and after in vivo heat stress of lactating holstein cows. cytochrome-c reduction and random migration of pmnl were reduced when cells were preincubated or incubated at 42 c, but high incubation temperature had little or no effect on phagocytosis and killing of escherichia coli. proliferation of lymphocytes was reduce ...19911662922
fibroblast adhesion to recombinant tropoelastin expressed as a protein a-fusion protein.a bovine tropoelastin cdna encoding exons 15-36 that includes the elastin-receptor binding site was expressed in escherichia coli as a fusion protein with protein a from staphylococcus aureus. after isolation of the fusion protein by affinity chromatography on ig-sepharose, the tropoelastin domain was separated from plasmid-pr1t2t-encoded protein a (protein a') by cnbr cleavage. cell-adhesion assays demonstrated specific adhesion to the recombinant tropoelastin. furthermore, the data indicate th ...19911996952
antibacterial activities in vitro and in vivo and pharmacokinetics of cefquinome (hr 111v), a new broad-spectrum cephalosporin.cefquinome is a new injectable aminothiazolyl cephalosporin derivative. it is stable against chromosomally and plasmid-encoded beta-lactamases and has a broad antibacterial spectrum. staphylococcus aureus, streptococci, pseudomonas aeruginosa, and members of the family enterobacteriaceae (escherichia coli, salmonella spp., klebsiella spp., enterobacter spp., citrobacter spp., and serratia marcescens) are inhibited at low concentrations. cefquinome is also active against many strains of methicill ...19912014969
molecular characterization of bovine brain p75, a high affinity binding protein for the regulatory subunit of camp-dependent protein kinase ii beta.in mammalian brain, physiological signals carried by camp seem to be targeted to intraneuronal sites by the association of camp-dependent protein kinase ii beta with anchoring proteins that bind the regulatory subunit (rii beta) of the enzyme. previously, an rii beta-binding domain was characterized in a large (mr approximately 150,000) candidate anchor protein, rat brain p150 (bregman, d. b., bhattacharyya, n., and rubin, c. s. (1989) j. biol. chem. 264, 4648-4656). rii beta-binding proteins wi ...19912016323
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