| the development of a selective medium for the brucella abortus strains and its comparison with the currently recommended and used medium. | the brucella spp. are fastidious and relatively slow-growing organisms. the isolation of such strains in a variety of specimens often requires the use of a selective medium to reduce or eliminate the growth of unexpected microorganisms. the modified brucella selective (mbs) medium, which contains improved antibiotic mixtures, erythritol as the only carbon source, and neutral red as a ph indicator, showed good selectivity for the brucella abortus strains, including the rb51 vaccine strain. erythr ... | 2010 | 20385349 |
| epidemiological study of bovine brucellosis in three agro-ecological areas of central oromiya, ethiopia. | a cross-sectional sero-epidemiological study of bovine brucellosis was conducted between september 2005 and march 2006 in three separate agroecological areas of central oromiya, ethiopia. in this study, a total of 176 clusters (farms) and 1,238 animals were selected, using the one-stage cluster sampling method. fifty-nine clusters and 423 animals were selected from the lowland areas; 58 clusters and 385 animals from the midland areas and 59 clusters and 430 animals from the highlands. serum samp ... | 2009 | 20462151 |
| seroprevalence of bovine brucellosis in trade cattle slaughtered in ibadan, nigeria, from 2004-2006. | a seroprevalence study was carried out among trade cattle slaughtered at bodija municipal abattoir, ibadan (southwestern nigeria) over a period of 3 consecutive years from 2004 to 2006 with a view to determining the breed, sex and age distribution in the seropositivity of bovine brucellosis. in total, 1642 animals were examined for antibodies to brucella abortus using the rose bengal test. seroprevalences of 6.00%, 6.17% and 5.31% were obtained in the years 2004, 2005 and 2006, respectively but ... | 2010 | 20649155 |
| heat-killed and γ-irradiated brucella strain rb51 stimulates enhanced dendritic cell activation, but not function compared with the virulent smooth strain 2308. | brucella spp. are gram-negative, facultative intracellular bacterial pathogens that cause abortion in livestock and undulant fever in humans worldwide. brucella abortus strain 2308 is a pathogenic strain that affects cattle and humans. currently, there are no efficacious human vaccines available. however, b. abortus strain rb51, which is approved by the usda, is a live-attenuated rough vaccine against bovine brucellosis. live strain rb51 induces protection via cd4(+) and cd8(+) t-cell-mediated i ... | 2010 | 20812951 |
| diagnostic and vaccine chapter. | the first report in this chapter describes the development of a killed composite vaccine. this killed vaccine is non-infectious to humans, other animals, and the environment. the vaccine has low reactivity, is non-abortive, and does not induce pathomorphological alterations to the organs of vaccinated animals. the second report of this chapter describes the diagnostic value of a competitive enzyme-linked immunosorbent assay for detecting brucella-specific antibodies and its ability to discrimina ... | 2010 | 20850688 |
| comparative analysis of the early transcriptome of brucella abortus - infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis. | brucellosis is a worldwide zoonotic infectious disease that has a significant economic impact on animal production and human public health. we characterized the gene expression profile of b. abortus-infected monocyte-derived macrophages (mdms) from naïve cattle naturally resistant (r) or susceptible (s) to brucellosis using a cdna microarray technology. our data indicate that (1) b. abortus induced a slightly increased genome activation in r mdms and a down-regulated transcriptome in s mdms, dur ... | 2010 | 20932540 |
| iron homeostasis in brucella abortus: the role of bacterioferritin. | brucella abortus is the etiological agent of bovine brucellosis, an infectious disease of humans and cattle. its pathogenesis is mainly based on its ability to survive and multiply inside macrophages. it has been demonstrated that if b. abortus ferrochelatase cannot incorporate iron into protoporphyrin ix to synthesize heme, the intracellular replication and virulence in mice is highly attenuated. therefore, it can be hypothesized that the unavailability of iron could lead to the same attenuatio ... | 2010 | 21046346 |
| a genetic comparison of brucella abortus isolates from animals and humans by using an mlva assay. | the mlva assay is known to have a high ability to identify and discriminate brucella species, so that it can be used as an epidemiological tool to discriminate brucella isolates originating from restricted geographic sources. in this study, the genetic profiles of 38 b. abortus isolates from humans were analyzed and compared with genotypes from animal isolates in south korea. as a result, it was found that they did not show high genetic diversity and were compacted. they were clustered together ... | 2010 | 21193833 |
| brucellosis in camels. | camels are highly susceptible to brucellosis caused by brucella melitensis and brucella abortus. difficulties can arise in diagnosis of camel brucellosis, especially as this disease provokes only few clinical signs in contrast to its clinical course in cattle. because none of the commonly used serological test can be perceived as a perfect test for brucella diagnosis in camel and most serological tests used for camels have been directly transposed from cattle without adequate validation, an inco ... | 2011 | 21632084 |
| effect of polymorphisms in the slc11a1 coding region on resistance to brucellosis by macrophages in vitro and after challenge in two bos breeds (blanco orejinegro and zebu). | the resistance/susceptibility of selected cattle breeds to brucellosis was evaluated in an f1 population generated by crossing animals classified as resistant (r) and susceptible (s) (r x r, r x s, s x r, s x s) based on challenges in vitro and in vivo. the association between single nucleotide polymorphisms identified in the coding region of the slc11a1 gene and resistance/susceptibility was estimated. the trait resistance or susceptibility to brucellosis, evaluated by a challenge in vitro, sho ... | 2010 | 21637417 |
| epidemiological aspects of an infection by brucella abortus in risk occupational groups in the microregion of araguaína, tocantins. | the aim of this paper was to study some epidemiological aspects of the infection by brucella abortus in risk occupational groups in the microregion of araguaína, tocantins. for antibody research, 645 serum samples were analyzed by the complement fixation test (cf). a 4.0% frequency was found (26/645) in patients' serum and among those 4.1% (23/551) were slaughterhouses employees and 8.1% (3/37) rural workers. of the total positive samples, three (2.0%) were women and 23 (4.7%) men; ten (2.9%) we ... | 2008 | 18641850 |
| bovine slc11a1 3' utr sscp genotype evaluated by a macrophage in vitro killing assay employing a brucella abortus strain. | the 3' untranslated region (3' utr) of the bovine natural resistance-associated macrophage gene (nramp1 or slc11a1) was genotyped in colombian creole blanco orejinegro (bon) (bos taurus) (n = 140) and zebu brahman (bos indicus) (z) (n = 20) cattle and their crosses (bon x zebu brahman [b x z] [n = 10]; zebu brahman x bon [z x b] [n = 10]), and in animals from a holstein x bon (h x b) (n = 10) cross. direct sequencing and single-strand conformation polymorphism analysis (sscp) helped in detecting ... | 2008 | 18717968 |
| seroprevalence of brucellosis in yaks (poephagus grunniens) in india and evaluation of protective immunity to s19 vaccine. | the present study was carried out to explore the seroprevalence of brucellosis in yaks of north-eastern hilly yak tracts of arunachal pradesh, india. of 374 animals tested, 23.79, 21.11 and 18.98% were found positive for brucellosis using avidin-biotin elisa (ab-elisa), rose-bengal plate test (rbpt) and standard tube-agglutination test (stat), respectively. the relative sensitivity and specificity for stat were 79.77 and 100%, respectively and the same for rbpt were 88.76 and 100%, respectively ... | 2009 | 18763048 |
| evaluation of three different antigens in an indirect enzyme-linked immunoassay for the detection of antibodies against brucella abortus srb51 in vaccinated heifers. | the live attenuated brucella abortus srb51 (srb51) is a partial o-chain-deprived mutant. the relative lack of the polysaccharide prevents it from inducing antibodies detectable by most of the serological tests used for the diagnosis of bovine brucellosis. the performance of three antigens used in an indirect enzyme-linked immunoassay test for detecting srb51 antibodies were evaluated. a homogeneous group of twenty-five 10-month-old hereford heifers was used. the animals were bled on day 0 and th ... | 2009 | 18980780 |
| a proficiency testing method for detecting antibodies against brucella abortus in quantitative and qualitative serological tests. | a proficiency testing panel for detecting antibodies against brucella abortus was developed and evaluated by both primary binding and conventional serological tests, using the guidelines of the world organisation for animal health and the international organization for standardization guide 43-1. all serological tests were judged satisfactory. among the primary binding tests, the competitive enzyme-linked immunosorbent assay (elisa 2) and the indirect enzyme-linked immunosorbent assay (elisa 1), ... | 2008 | 19284049 |
| eradication of bovine brucellosis in the azores, portugal-outcome of a 5-year programme (2002-2007) based on test-and-slaughter and rb51 vaccination. | bovine brucellosis is an important contagious disease that can cause abortions and infertility in cattle, and can be transmitted to humans. despite having an eradication programme in place since 1994, in 2000 the situation of bovine brucellosis due to brucella abortus was not significantly improving in 3 of the 9 islands (terceira, s. miguel and s. jorge) of the archipelago of azores, an autonomous region of portugal. farming on these islands, particularly dairy, is extensive. therefore, the use ... | 2009 | 19439382 |
| effects of brucella abortus biotype 1 infection on the reproductive performance of sprague-dawley rats. | the aim of this study was to assess the impact of brucella abortus biotype 1 infection on the reproductive performance using sprague-dawley (sd) rat model. virgin female sd rats (n = 24) were infected intraperitoneally with 0.1 ml of saline containing 1 x 10(11) colony forming unit (cfu) of pathogenic b. abortus biotype 1 korean bovine isolate. control rats (n = 24) were inoculated with 0.1 ml of apyrogenic saline. both inoculated and control rats were divided into six subgroups. four rats in ea ... | 2009 | 19579969 |
| immune responses and efficacy after administration of a commercial brucella abortus strain rb51 vaccine to cattle. | brucella abortus strain rb51 (srb51) is a newly approved live vaccine to protect cattle against brucellosis. the purpose of this study was to evaluate the immunologic responses of cattle to a commercially available srb51 vaccine and to characterize the efficacy of the vaccine to protect against abortion or infection after midgestational challenge with virulent b. abortus strain 2308 (s2308). all cattle were pasture bred, and pregnancy was confirmed by rectal palpation. pregnant cattle were intra ... | 2000 | 19757581 |
| bovine brucellosis. | infection of cattle caused by brucella abortus (ie, bovine brucellosis) has been of political importance in the united states for many decades. the most common clinical manifestation of brucellosis in natural hosts is reproductive loss resulting from abortion, birth of weak offspring, or infertility. brucellosis regulatory programs were primarily developed as the most efficient way to prevent human infections. this article discusses cattle vaccination with b abortus strains 19 and rb51. other re ... | 2010 | 20117540 |
| reply to letter to the editor by blasco and moriyon (2009) concerning the manuscript "eradication of bovine brucellosis (bb) in the azores, portugal-outcome of a 5-year programme (2002-2007) based on test-and-slaughter and rb51 vaccination" by h. martins et al. (2009). | | 2010 | 20079547 |
| preliminary assessment of binary ethylenimine inactivated and saponized cutaneous warts (bpv-2) therapeutic vaccine for enzootic bovine haematuria in hill cows. | a preliminary therapeutic vaccine trial was conducted in hill cows to evaluate the therapeutic potential of binary ethylenimine (bei) inactivated and saponized bovine papillomavirus-2 (bpv-2) for enzootic bovine haematuria (ebh). although the vaccine failed to show favorable clinical vaccine results in treatment of ebh affected cows at 120 days post-vaccination but immunopathological responses were encouraging. a significant difference was observed in humoral (against brucella abortus strain 19s ... | 2011 | 21803104 |