seroprevalence of neospora caninum and brucella abortus among dairy cattle herds with high abortion rates.abstract the protozoon neospora caninum and the bacterium brucella abortus are well recognized abortifacients in dairy cattle. serum samples (n = 240) from aborting (n = 141) and at risk (n = 99) animals from 5 herds with high abortion rates in punjab province, pakistan were tested for antibodies to n. caninum using monoclonal antibody based competitive elisa and to b. abortus using the serum agglutination test. antibodies to n. caninum and b. abortus were detected in 105 (43.8 %) and 135 (56.3% ...201121506829
effects of brucella abortus biotype 1 infection on the reproductive performance of sprague-dawley rats.the aim of this study was to assess the impact of brucella abortus biotype 1 infection on the reproductive performance using sprague-dawley (sd) rat model. virgin female sd rats (n = 24) were infected intraperitoneally with 0.1 ml of saline containing 1 x 10(11) colony forming unit (cfu) of pathogenic b. abortus biotype 1 korean bovine isolate. control rats (n = 24) were inoculated with 0.1 ml of apyrogenic saline. both inoculated and control rats were divided into six subgroups. four rats in ea ...200919579969
reply to letter to the editor by blasco and moriyon (2009) concerning the manuscript "eradication of bovine brucellosis (bb) in the azores, portugal-outcome of a 5-year programme (2002-2007) based on test-and-slaughter and rb51 vaccination" by h. martins et al. (2009). 201020079547
epidemiological study of bovine brucellosis in three agro-ecological areas of central oromiya, ethiopia.a cross-sectional sero-epidemiological study of bovine brucellosis was conducted between september 2005 and march 2006 in three separate agroecological areas of central oromiya, ethiopia. in this study, a total of 176 clusters (farms) and 1,238 animals were selected, using the one-stage cluster sampling method. fifty-nine clusters and 423 animals were selected from the lowland areas; 58 clusters and 385 animals from the midland areas and 59 clusters and 430 animals from the highlands. serum samp ...200920462151
phenotypic and genotypic characterisation of brucella strains isolated from cattle in the gambia.thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the western region of the gambia in order to isolate and characterise brucella species. information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. twenty-six (74 per cent) of the serum samples were positive in the rose bengal ...201020543166
status of brucellosis in free-ranging elk and bison in wyoming.brucella abortus is the causative agent of brucellosis, a disease enzootic in populations of free-ranging elk (cervus elaphus) and bison (bison bison) in the greater yellowstone ecosystem, usa. we define the distribution of the disease in elk throughout wyoming, usa, using three epidemiologic/geographic classifications based on winter-foraging opportunity. antibody prevalence for >3,300 yearling and adult, female elk, sampled from supplemental feedgrounds in western wyoming, usa, since 1985, was ...201020688637
heat-killed and γ-irradiated brucella strain rb51 stimulates enhanced dendritic cell activation, but not function compared with the virulent smooth strain 2308.brucella spp. are gram-negative, facultative intracellular bacterial pathogens that cause abortion in livestock and undulant fever in humans worldwide. brucella abortus strain 2308 is a pathogenic strain that affects cattle and humans. currently, there are no efficacious human vaccines available. however, b. abortus strain rb51, which is approved by the usda, is a live-attenuated rough vaccine against bovine brucellosis. live strain rb51 induces protection via cd4(+) and cd8(+) t-cell-mediated i ...201020812951
[molecular characterization of brucella abortus strain 19 and its application for controlling biologics].brucella abortus is the etiological agent of bovine brucellosis. the strain 19 used in vaccine elaboration can be identified through a deletion in the eri region associated with its susceptibility to erythritol. we optimized a pcr assay for specific characterization of this strain. the method described here is a rapid procedure that enables identification of b. abortus, and simultaneous differentiation of the strain 19 from other b. abortus biovar 1 strains. we applied the assay to detect the st ...200516323658
brucellosis in camels.camels are highly susceptible to brucellosis caused by brucella melitensis and brucella abortus. difficulties can arise in diagnosis of camel brucellosis, especially as this disease provokes only few clinical signs in contrast to its clinical course in cattle. because none of the commonly used serological test can be perceived as a perfect test for brucella diagnosis in camel and most serological tests used for camels have been directly transposed from cattle without adequate validation, an inco ...201121632084
failure to isolate brucella abortus from embryos or ova from culture-positive superovulated cows.nine, brucella abortus culture positive 2-yr-old cows were used to test the hypothesis that embryos and ova collected from such cows are not infected. superovulation was induced at varying times postpartum or postabortion with intramuscular injections of follicle stimulating hormone (fsh). the cows were artificially inseminated with b. abortus-negative semen. superovulations and nonsurgical embryo collections nonsurgical embryo collections were attempted twice for each cow. jugular blood, udder ...198816726359
variable-number tandem repeat markers for identification of brucella abortus 82 and 75/79-av vaccine russia, live vaccine strains brucella abortus 82 and b. abortus 75/79-av have been widely and extensively utilized for specific prophylaxis of cattle brucellosis. to differentiate these vaccine strains from each other and laboratory collection of other vaccine (n=4), reference (n=15) and field brucella strains (n=61), the multiple-locus variable-number tandem repeat (vntr) analyses (mlva) were used with 12 loci containing tandem repeats from 134bp to 8bp recently described for brucella spp. t ...201020362200
comparative study of the immunobiological properties of live brucellosis vaccines.findings from the comparative study of the immunobiological properties of live brucellosis vaccines in guinea pigs are presented in the article. vaccines from strains brucella abortus 19 (u.s.) and 82 (russia), in the s and sr forms, respectively, exhibited the highest and most pronounced immunological efficacy, while vaccines from strains b. abortus 82-ps (in the rs form) and b. abortus rb-51 and 75/79-ab (in the r forms) exhibited the lowest. the live vaccine from strain b. abortus 82, togethe ...201020362201
designing vaccines against cattle brucellosis.the article presents the results from a study of two new vaccines against cattle brucellosis from brucella abortus cultures. one of these is a live dry vaccine prepared from a weakly agglutinogenic strain in the sr form and the second, an inactivated adjuvant vaccine from an non-agglutinogenic strain in the r form. the immunogenic properties of the vaccines were studied in three tests on heifers with infection by a virulent b. abortus culture. the vaccines passed the farm-scale testing and were ...201020362619
serological evidence of brucella abortus prevalence in punjab province, pakistan--a cross-sectional study.twenty-one villages in punjab province, seven from each region (north, central& south) were randomly selected to determine the prevalence of b. abortus infection by two age categories (group a: 0–2 years; group b: >2 years) in cattle and buffalo populations. in each village, eight blood samples were collected from each age group of cattle and buffaloes. sera from a total of 672 blood samples (336 each from cattle and buffaloes) were analysed for the presence of antibodies to b. abortus using ros ...201021117286
serological discrimination by indirect enzyme immunoassay between the antibody response to brucella sp. and yersinia enterocolitica o:9 in cattle and pigs.a rapid, inexpensive and rugged serological test that distinguishes cattle and swine infected with brucella sp. or yersinia enterocolitica o:9 is described. the test protocol, which is an indirect enzyme immunoassay uses a high concentration of divalent cation chelating agents to minimize binding of y. enterocolitica o:9 antibody to rough lipopolysaccharide antigen derived from b. abortus rb51. no false positive reactions were observed when testing 100 canadian cattle and swine without any evide ...200616140390
comparative analysis of the early transcriptome of brucella abortus - infected monocyte-derived macrophages from cattle naturally resistant or susceptible to brucellosis.brucellosis is a worldwide zoonotic infectious disease that has a significant economic impact on animal production and human public health. we characterized the gene expression profile of b. abortus-infected monocyte-derived macrophages (mdms) from naïve cattle naturally resistant (r) or susceptible (s) to brucellosis using a cdna microarray technology. our data indicate that (1) b. abortus induced a slightly increased genome activation in r mdms and a down-regulated transcriptome in s mdms, dur ...201020932540
brucella abortus infection in cattle; agglutination tests. 194620988177
effect of polymorphisms in the slc11a1 coding region on resistance to brucellosis by macrophages in vitro and after challenge in two bos breeds (blanco orejinegro and zebu).the resistance/susceptibility of selected cattle breeds to brucellosis was evaluated in an f1 population generated by crossing animals classified as resistant (r) and susceptible (s) (r x r, r x s, s x r, s x s) based on challenges in vitro and in vivo. the association between single nucleotide polymorphisms identified in the coding region of the slc11a1 gene and resistance/susceptibility was estimated. the trait resistance or susceptibility to brucellosis, evaluated by a challenge in vitro, sho ...201021637417
preliminary assessment of binary ethylenimine inactivated and saponized cutaneous warts (bpv-2) therapeutic vaccine for enzootic bovine haematuria in hill cows.a preliminary therapeutic vaccine trial was conducted in hill cows to evaluate the therapeutic potential of binary ethylenimine (bei) inactivated and saponized bovine papillomavirus-2 (bpv-2) for enzootic bovine haematuria (ebh). although the vaccine failed to show favorable clinical vaccine results in treatment of ebh affected cows at 120 days post-vaccination but immunopathological responses were encouraging. a significant difference was observed in humoral (against brucella abortus strain 19s ...201121803104
iron homeostasis in brucella abortus: the role of bacterioferritin.brucella abortus is the etiological agent of bovine brucellosis, an infectious disease of humans and cattle. its pathogenesis is mainly based on its ability to survive and multiply inside macrophages. it has been demonstrated that if b. abortus ferrochelatase cannot incorporate iron into protoporphyrin ix to synthesize heme, the intracellular replication and virulence in mice is highly attenuated. therefore, it can be hypothesized that the unavailability of iron could lead to the same attenuatio ...201021046346
identification of new is711 insertion sites in brucella abortus field isolates.abstract: background: brucellosis is a zoonosis caused by brucella spp., a group of highly homogeneous bacteria. the insertion sequence is711 is characteristic of these bacteria, and occurs in variable numbers and positions, but always constant within a given species. this species-associated polymorphism is used in molecular typing and identification. field isolates of b. abortus, the most common species infecting cattle, typically carry seven is711 copies (one truncated). thus far, is711 transp ...201121813003
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