| characterization of an atypical biotype of brucella abortus. | brucella abortus strains were isolated from bovine tissue and milk samples from seven ontario herds. the isolates were characterized by colonial morphology, requirement of co2 for growth, lysis by tbilisi phage, biochemical tests and agglutination in monospecific sera. they resembled b. abortus biotype 2 (on the basis of sensitivity to thionin and basic fuchsin) and biotype 4 (on the basis of agglutination with anti-brucella "m" but not anti-brucella "a" absorbed sera). sodium dodecyl sulphate-p ... | 1988 | 3139274 |
| structural and immunochemical aspects of brucella abortus endotoxins. | smooth lipopolysaccharide (slps) of brucella abortus, which is the most immunodominant component among the antigens of b. abortus isolated, has been used for diagnosis for decades. high yields of slps can be prepared by a modification of the procedures of moreno et al. (j. bacteriol. 138:361-369, 1979). washed b. abortus cells can be disrupted by 21 freeze-quick thaw cycles and ultrasonication to separate non-membrane-bound material; then phenol extraction is performed 3 times and the phenol fra ... | 1988 | 3140612 |
| distribution of brucella abortus organisms in calves after conjunctival exposure. | thirty calves (3 to 4 months old) were exposed conjunctivally to a pathogenic strain of brucella abortus. calves were euthanatized and necropsied at postexposure hours 2 and 4, and at postexposure days (ped) 1, 4, 7, 14, 21, 42, and 49. selected ocular, pharyngeal, and lymphoid tissues were cultured bacteriologically for brucellae to determine organism distribution. brucella abortus organisms initially localized in the third eyelids, bulbar conjunctivae, and parotid lymph nodes and were detected ... | 1988 | 3149162 |
| further evidence that bovine igm does not fix guinea pig complement. | in a study of sera from cattle vaccinated with 3 x 10(10) cfu of brucella abortus strain 19, it was found that igg1 antibody measured by an indirect elisa was the only isotype to correlate with standard complement fixing antibody titers using heated serum samples and guinea pig serum as a source of complement. a supplement of normal unheated bovine serum resulted in igm fixing guinea pig complement, giving data similar to those obtained with unheated serum in the complement fixation test. | 1988 | 3150884 |
| contact dermatitis caused by brucella. | we report a case of a distinctive dermatitis in a dairy worker exposed to brucella abortus while manually delivering an infected calf. the rapid onset of pruritus, the strict limitation of lesions to the upper extremities, and the negative results on growth of culture for microorganisms make direct bacterial infection an untenable cause in this case. allergic hypersensitivity is the probable mechanism, manifesting itself initially as contact urticaria. coincidentally our patient also had systemi ... | 1985 | 3158485 |
| resistance of preimplantation bovine embryos to infection with brucella abortus. | preimplantation bovine embryos were exposed in vitro to brucella abortus to determine if the bacteria would adhere to zona pellucida (zp)-intact embryos or adhere to or infect zp-free embryos. brucella abortus was not isolated from zp-intact or zp-free groups of embryos after 10 sequential antibiotic-free washings. brucella abortus was isolated from all groups containing zp-defective embryos after the exposure period and washing. detrimental effects on healthy in vitro development of embryos wer ... | 1986 | 3767099 |
| assessment of bovine mammary gland macrophage oxidative burst activity in a chemiluminescence assay. | a major bactericidal mechanism of neutrophils and macrophages is the generation of toxic oxygen-free radicals upon phagocytosis of microbes. studies were conducted to assess the oxidative metabolism of bovine mammary gland macrophages. bovine mammary gland macrophages were challenge exposed with a variety of phagocytic stimuli in an in vitro, luminol-assisted chemiluminescence assay. a measurable oxidative burst was observed when macrophages were challenge exposed with heat-aggregated bovine imm ... | 1987 | 3826830 |
| a competitive enzyme immunoassay for the detection of bovine antibodies to brucella abortus using monoclonal antibodies. | a competitive enzyme immunoassay (eia) for the detection of circulating bovine antibodies to brucella abortus has been developed using horseradish peroxidase conjugated monoclonal antibodies (mab) raised against b. abortus cell surface antigens. antibodies present in the serum of either vaccinated or infected cattle can apparently displace the conjugated mab from the lipopolysaccharide antigen (lps) in a quantitatively different manner allowing an assessment of immune status of the animal. the r ... | 1985 | 3922105 |
| delayed-type hypersensitivity responses induced by bovine colostral components. | transfer factor-type substances obtained from leukocytic cells and fluid portions of bovine colostrum caused effective passive transfer of delayed-type hypersensitivity responses across species barriers. passive transfer of brucella abortus sensitivity was obtained with equal regularity when using components derived from peripheral blood and colostrum of dams sensitized at 3 and 9 months of age. colostral feedings to calves caused the passive transfer of delayed-type hypersensitivity as early as ... | 1985 | 3925823 |
| profiles of serological reactions following adult cow inoculation with standard dose brucella abortus strain 19 vaccine. | experimental use of the standard dose (4-12 x 10(10) viable organisms per dose) brucella abortus strain 19 vaccine (s19) in adult cattle led to a serological response in the complement fixation test (cft) within 2 weeks. the antibody titres recorded could not be distinguished from those arising from infection for a period of 6-11 months. this was in the absence of the booster effect of brucella antigen from either field or vaccine strains. the illegal or unintentional use of s19 in adult cattle ... | 1985 | 3927001 |
| a "dipstick" enzyme immunoassay for detection of antibody to brucella abortus in cattle sera. | an enzyme immunoassay that utilizes antigen bound to a matrix which can be removed from the substrate to stop development is described. the assay which is performed in glass or plastic disposable tubes uses gel-bond film strips for attachment of antigen. the only equipment requirements are a rotary shaker and a spectrophotometer (optional). the antigen coated strips are passed through a series of tubes containing test serum, wash solution, antibody-enzyme conjugate, wash solution and substrate-c ... | 1985 | 3930057 |
| application of a timing protocol to the reduction of inter-plate variability in the indirect enzyme immunoassay for detection of anti-brucella antibody. | a simple timing protocol was developed to monitor chromogen conversion in an enzyme immunoassay, performed in microtiter plates, for the detection of antibody to brucella abortus in bovine serum. application of this protocol decreased the inter-plate coefficient of variation from 28.6% to 6.8% when optical density (od) values, subsequent to the reaction of a standard antibody reagent, were compared to a static development time. substantial reductions in variation were also observed for low titer ... | 1985 | 3930575 |
| evaluation of brucellin skin test for bovine brucellosis. | the diagnostic efficiency of the brucellin skin test for bovine brucellosis was evaluated using cattle of known history. the test was negative in six out of 14 heifers (42.9%) infected with virulent brucella abortus (br. abortus) strain 544. in four cattle vaccinated with a reduced dose of br. abortus strain 19 vaccine (5 x 10(9) live organisms) the skin test became positive in all the animals but two weeks after immunization. however, all the vaccinates became negative 14 weeks after vaccinatio ... | 1985 | 3932248 |
| the instability of brucella abortus strain 45/20 and a note on significance of using an unstable rough strain in the diagnosis of bovine brucellosis. | the serum agglutinin titres of six heifers experimentally inoculated with live br. abortus strain 45/20 were similar to those produced in six bullocks inoculated with virulent br. abortus strain 544. however, the agglutinin response appeared slowly and disappeared earlier in the heifers than in the bullocks. it is concluded that br. abortus strain 45/20 is an unstable strain, could revert to the smooth form and become highly agglutinogenic in non-pregnant heifers. the significance of using an un ... | 1985 | 3935591 |
| [detection in bovine serum of non-agglutinating antibodies directed against occult antigens of brucella abortus strain 19]. | injection of different animal species with particulate t-dependent antigens induces the appearance of two antibody populations belonging to igg class, one of them with agglutinating activity and the other lacking this capacity, that is, non-agglutinating. in this paper, 3 adult female hereford were inoculated subcutaneously, fortnightly, four times, with 5 x 10(10) heat killed smooth s19 brucella abortus cells. blood samples were taken ten days after the last inoculation and were individually pr ... | 1985 | 3939693 |
| [typing of 800 strains of brucella abortus isolated from aborted cattle embryos]. | | 1964 | 4157875 |
| the hygiene and marketing of fresh cream as assessed by the methylene blue test. a report by a working party to the director of the public health laboratory service. | the hygiene and marketing of fresh cream in england and wales was investigated by a working party of the public health laboratory service (phls) between 1 october 1968 and 31 july 1969. thirty-one cream-producing dairies were visited and observations made in the light of the code of practice published by the milk and milk products technical advisory committee of the ministry of agriculture, fisheries and food, and the scottish home and health department. suggestions are made in this report to st ... | 1971 | 4326247 |
| immune response to porin in cattle immunized with whole cell, outer membrane, and outer membrane protein antigens of brucella abortus combined with trehalose dimycolate and muramyl dipeptide adjuvants. | the immune response of cattle to nonliving vaccines derived from brucella abortus rough strain 45/20 was studied. vaccines contained trehalose dimycolate and a derivative of muramyl dipeptide. n-acetylmuramyl-l-alpha-aminobutyryl-d-isoglutamine. a factorial experiment was designed to test the effects of type of antigen, quantity of antigen, and quantity of mineral oil on the immune response to porin. muramyl dipeptide was kept constant at 5 mg per dose, and 1 part of trehalose dimycolate was inc ... | 1983 | 6315592 |
| vaccination of cattle against brucellosis using either a reduced dose of strain 19 or one or two doses of 45/20 vaccine. | three groups, each of 14 mature jersey heifers, were vaccinated. they were mated about 2 months later and those that became pregnant were challenged at about 6.5 months of pregnancy by the conjunctival application of virulent brucella abortus. group 1 heifers received 2 doses of b. abortus 45/20 vaccine 2 months apart. only 5 of the 14 heifers became pregnant, and of these 5 only one resisted challenge. group 2 heifers received only one dose of 45/20 vaccine, 5 of the 10 challenged resisted infe ... | 1983 | 6626063 |
| selective suppression by auto-anti-idiotypic antibody of b-cell idiotype repertoires generated after stimulation with the same hapten on t-dependent and t-independent carriers. | in the present study, we investigated whether auto-anti-idiotypic antibody in the immune sera from old mice could recognize antitrinitrophenyl (tnp) plaque-forming cells (pfc) generated after stimulation with the t-dependent and t-independent forms of the hapten, tnp. young and old c57bl/6j male mice were immunized with a variety of t-dependent (tnp-bovine gamma-globulin, tnp-bgg; tnp-keyhole limpet hemocyanin, tnp-klh; ovalbumin, ova; bovine serum albumin, bsa; bgg) and t-independent (tnp-bruce ... | 1983 | 6360382 |
| comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis. | sera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with brucella abortus strain 19 was compulsory. of 4583 sera positive by the rose bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. these figures, and supporting evidence from the eradication programme, ... | 1983 | 6401777 |
| detection of brucella antibodies of different immunoglobulin classes in cow milk by enzyme-linked immunosorbent assay. | enzyme-linked immunosorbent assays were conducted on milk of cows from which brucella abortus was isolated and that of noninfected controls. horseradish peroxidase-labeled rabbit antibovine immunoglobulins igg, igg1, and iga were used as conjugates. a heat-killed whole-cell suspension of b abortus strain 19 was used as the antigen. differences in antibody profiles were observed in milk of cows from which b abortus was isolated and in milk of noninfected cows. antibody profiles were similar in mi ... | 1983 | 6402961 |
| an evaluation of the anamnestic test for brucellosis in cattle of the northern pastoral area. | an investigation of the anamnestic test for brucellosis using brucella abortus 45/20 vaccine was carried out in 3 groups of weaner cattle on 2 farms in western queensland. each group originally consisted of about 500 cattle. they were bled before and at 6 or 10 weeks after vaccination and again in the following year. the serums were tested by the complement fixation (cft), rose bengal (rbt) and indirect haemolysis tests (ihlt). most of the cattle reacting to one or more of the tests were killed ... | 1983 | 6403007 |
| the cultural and pathological examination of bulls serologically positive for brucellosis. | the genitalia from 34 bulls were examined for the presence of brucella lesions using histological, bacteriological and serological methods. tissue fluids extracted from the various genital organs and the serums were examined using the serum agglutination test (sat), the rose bengal plate agglutination test (rbpt), the complement fixation test (cft) and the indirect haemagglutination test (ihlt) for the presence of brucella antibody. titres to one or more of these tests were found in 17 bulls and ... | 1983 | 6403010 |
| ethylenediaminetetraacetic acid (disodium salt)-labile bovine immunoglobulin m fc binding to brucella abortus: a cause of nonspecific agglutination. | it was demonstrated by a radioimmunoassay procedure that brucella abortus agglutinins from noninfected cattle sera, absorbed to b. abortus antigen and eluted with ethylenediaminetetraacetic acid (edta), was immunoglobulin m that bound to that bacterium by its fc portion. the edta-eluted immunoglobulin m agglutinated intact b. abortus cells but not erythrocytes treated with b. abortus lipopolysaccharide. the specificity of the edta-eluted immunoglobulin was for b. abortus, although a small titer ... | 1981 | 6790568 |
| [production of dialysable leukocytes extracts in cattle (author's transl)]. | | 1981 | 6793294 |
| assessment of an intradermal test for the detection of bovine brucellosis. | forty-eight cattle were sensitised to brucella antigens either by vaccination with brucella abortus strain 19 (s19) or b. abortus 45/20 (s45/20) ad 24 of these and 12 unvaccinated cattle were subsequently challenged with virulent b. abortus strain 544 (s544). all these cattle (n = 60), together with 12 control cattle which were neither vaccinated nor challenged, were subsequently subjected to an intradermal test using a s45/20 protein antigen. reactions were interpreted subjectively by observati ... | 1983 | 6417883 |
| studies on the adjuvant action of beryllium. ii. systemic effects with particular reference to secretory immunity. | when 5 mg doses of beryllium hydroxide, be(oh)2, were injected intravenously (i.v.) into rats, much of the injected material was retained in the lungs. when the injection of be(oh)2 was accompanied by particulate antigens such as killed b. abortus organisms or srbc, immune responses took place in the intra-thoracic lymph nodes (itln) so that antibodies of the iga class were generated and endowed the bile with significant agglutinating activity. this did not happen when the antigens were injected ... | 1984 | 6432684 |
| comparison of sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles and antigenic relatedness among outer membrane proteins of 49 brucella abortus strains. | outer membrane proteins were solubilized from 49 strains of brucella abortus by sequential extraction of physically disrupted cells with n-lauroylsarcosinate and a dipolar ionic detergent (verstreate et al., infect. immun. 35:979-989, 1982). the strains tested included standard agglutination test strain 1119, virulent strain 2308, and eight reference strains representing each of the biotypes; the remainder were isolates from cattle in north america with natural infections and included biotypes 1 ... | 1984 | 6434426 |
| the use of a brucella protein antigen in dermal hypersensitivity as an adjunct method to diagnose bovine brucellosis. | a total of 191 cows in 3 herds were injected with a brucella protein antigen in an effort to compare serologic and bacteriologic findings with delayed hypersensitivity and to possibly detect incubative brucellosis. using cultural and serologic results, the responses to the bpa antigen were inferior to serologic tests in the diagnosis of infection. none of the 23 skin test positive/seronegative cows were positive on subsequent herd blood tests. | 1983 | 6419446 |
| latent bovine brucellosis. | adult cattle in a herd suffering from widespread infection with brucella abortus biotype 2 were slaughtered during the eradication campaign. a heifer calf fom that herd was moved to a fresh herd which remained apparently free from brucellosis until nine years later when the same animal produced a strongly positive serological reaction and brucella abortus biotype 2 was isolated from its milk. | 1982 | 7157620 |
| [characteristics of 273 strains of brucella abortus of african origin]. | 273 brucella strains from africa have been examined for identification and typing in our laboratory, since 1976. of these, 213 were isolated from senegal, 30 from togo, 12 from morocco, 10 from rwanda, 7 from guinea bissau and 1 from niger. 272 strains were from cattle. most of them (260) were from native animals that showed hygromas. 12 strains from morocco were isolated from aborted calves of the "pie noire" dairy breed. one strain was from a human case of brucellosis in rwanda. all the strain ... | 1984 | 6436118 |
| recent progress in the diagnosis of brucellosis. | progress since 1975 in the development of methods for the diagnosis of brucellosis is summarized. standard serological diagnosis improved with increased use of acidified antigen agglutination and complement fixation tests. immunoassays, tests based on the lysis of lipopolysaccharide coated erythrocytes and tests using new antigens have increased the sensitivity and specificity of serological results. the field of cellular immunology has seen the development and field evaluation of a skin test us ... | 1984 | 6436099 |
| [brucella abortus biotypes found in cattle in morocco: a preliminary study]. | | 1984 | 6436089 |
| analysis and in vivo assay of b. abortus agglutinating and non-agglutinating antibodies. | studies were made of physicochemical and immunochemical characteristics of brucella abortus agglutinating and non-agglutinating antibodies in the sera of cattle repeatedly injected with living b. abortus (strain 1119). both agglutinating and non-agglutinating antibody were shown to be igg1, and by immunodiffusion against rabbit anti-cattle gamma-globulin, agglutinating antibody gave a precipitation line of identity with that given by non-agglutinating antibody. whilst agglutinating antibody incr ... | 1984 | 6437058 |
| brucellacidal activity of human and bovine polymorphonuclear leukocyte granule extracts against smooth and rough strains of brucella abortus. | the microbicidal activities of freeze-thaw and high-salt extracts of human and bovine polymorphonuclear leukocyte (pmn) granules were tested against a smooth intermediate strain (45/0) and a rough strain (45/20) of brucella abortus which differ in virulence and survival within pmns. freeze-thaw extracts of human pmn granules were more brucellacidal than high-salt extracts when supplemented with hydrogen peroxide (h2o2) and potassium iodide (ki), whereas the opposite was found with freeze-thaw an ... | 1984 | 6090316 |
| simultaneous immunization of cattle and with strain 19 brucella abortus and infectious bovine rhinotracheitis (ibr) vaccines. | | 1965 | 5212981 |
| insulin-specific tolerance induction. ii. tolerant helper t cells can be rescued by insulin complexed to brucella abortus. | murine antibody responses to heterologous insulins are under h-2-linked immune response (ir) gene control. we previously demonstrated that the immune response to insulin in freund's complete adjuvant (cfa) can be specifically inhibited by prior injection of soluble insulin i.v. unresponsiveness requires at least 4 days after i.v. injection to develop, and once induced, it lasts 4 wk or more. unresponsiveness is caused by t cell, but not b cell, tolerance; furthermore, we have been unable to demo ... | 1984 | 6208264 |
| the use of milk and blood tests to identify cows excreting brucella abortus in their milk in brucellosis problem herds. | | 1965 | 5213210 |
| an association between arthritis and persistent serological reactions to brucella abortus in cattle from apparently brucellosis-free herds. | twenty-four cases of stifle (femoro-tibial joint) arthritis, in friesian heifers that had failed their first blood test for brucellosis, were examined pathologically and by serological and cultural tests for brucella. the arthritis was confined to the stifle and was non-purulent. the cases showed several other similar features, including evidence of trauma, erosions of articular surfaces, synovial proliferations and granulomata containing germinal centres and plasma cells. the synovial fluid was ... | 1980 | 6767307 |
| allergenic activity and biochemical analysis of three soluble antigen preparations from brucella abortus strain 45/20. | three brucella abortus strain 45/20 antigen preparations were compared with regard to their chemical composition and allergenic potential. a popular method of extracting soluble antigens from whole cells with hypertonic saline solution and ethanol precipitation was compared with the method of trichloroacetic acid (tca) or ammonium sulfate (as) precipitation of antigens released into solution by ultrasonic cell disruption. biochemical analysis of the preparations revealed that tca and as precipit ... | 1980 | 6767424 |
| an epidemiological survey of human brucellosis in three victorian abattoirs. | during the period 1977-1978, a serological and epidemiological investigation was conducted by the victorian departments of health and agriculture to determine the risk to abattoir workers of exposure to brucella abortus from infected cattle. nearly 1000 specimens of serum from workers at three abattoirs were tested for brucella antibodies at the microbiological diagnostic unit. serum agglutination and antihuman globulin tests were performed by a microtitre technique. approximately 25% of these s ... | 1980 | 6774222 |
| depressed immunoconglutinin responses in calves experimentally infected with trypanosoma congolense. | contrary to expectation, immunoconglutinin levels failed to rise significantly in calves infected with trypanosoma congolense. in addition, it was shown that trypanosome infection appeared to inhibit the immunoconglutinin response to brucella abortus strain 19. the possible reasons for these findings are discussed. | 1980 | 6774392 |
| protection of chickens by ribosomal vaccines from pasteurella multocida: dependence on homologous lipopolysaccharide. | chickens were protected against fowl cholera by ribosomal vaccines prepared from noncapsulated pasteurella multocida. passive hemagglutination (pha) titers to lipopolysaccharide (lps) and the degree of protection conferred by ribosomal vaccines were diminished or abolished when ribosomes were chromatographed on an immunoadsorbent column. addition of subimmunogenic amounts of serotype 1 (homologous) lps to highly purified ribosomes resulted in vaccines that protected against challenge exposure an ... | 1984 | 6208828 |
| the serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests. | serum sample were obtained from 281 heifers vaccinated with brucella abortus strain 19, and from 50 heifers that had received two injections of killed b. abortus strain 45/20 adjuvant (k45/20a) vaccine. the serological response measured by the brucellosis radioimmunoassay (ria) was compared with responses measured by other tests. the serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions. in the ... | 1982 | 6799566 |
| localization of brucella antigens that elicit a humoral immune response in brucella abortus-infected cells. | localization of brucella antigens, to which brucella-infected cattle make antibodies, and the surface characteristics of brucella abortus smooth strain 19 and rough strain 45/20 were studied by the use of monospecific antisera in absorption tests, electron microscopy, and electrophoretic mobility of organisms in microelectrophoresis. antigenic determinants of electrophoretically defined antigen a5 were present on the surface of b. abortus rough strain 45/20 organisms, and protein moieties were m ... | 1981 | 6800949 |
| the effect of challenge with virulent brucella abortus on beef cattle vaccinated as calves or adults with either brucella abortus strain 19 or 45/20. | groups of female calves were vaccinated subcutaneously with the standard dose of brucella abortus strain 19 (s19) or with b. abortus 45/20 (s45/20). these calves and non-vaccinated control calves were mated at 15 months of age and challenged by way of the conjunctival sac with b. abortus strain 544 (s544). the incidence of abortion, stillbirths, weanling calves and healthy calves was observed after challenge and specimens were collected for culture at parturition and slaughter. fifteen healthy c ... | 1981 | 6802119 |
| prevention of pathophysiologic and immunomodulatory effects of gastrointestinal nematodiasis in calves by use of strategic anthelmintic treatments. | effects of strategic anthelmintic treatment on pathophysiologic and immunomologic changes induced by infection with ostertagia ostertagi and cooperia oncophora were studied in 2 groups, of 12 calves each: an infected group, inoculated with 200,000 mixed o ostertagi and c oncophora third-stage larvae (l3) on day 1; and an infected-treated group, similarly inoculated, but treated with ivermectin at 9 and 33 days. all calves were also inoculated at 12 weeks with brucella abortus vaccine, at 13 week ... | 1993 | 8116936 |
| immunochemical studies on the binding properties of brucella abortus lipopolysaccharides to bovine precipitating antibodies. | the binding properties of brucella abortus smooth lipopolysaccharides (lps) to the precipitating antibodies in the serum of a b. abortus s2308 infected cow were studied by quantitative precipitin and precipitin-inhibition assays. crude b. abortus lipopolysaccharides (clps) and chemical modification products of strains 1119.3, 19 and 2308 (virulent) were used for this study. in the quantitative precipitin assay, 13 micrograms of clps precipitated about 4.0-5.2 micrograms nitrogen of precipitating ... | 1984 | 6083449 |
| transfer of memory cells into antigen-pretreated hosts. ii. influence of localized antigen on the migration of specific memory b cells. | following i.v. injection, 2,4,6-trinitrophenol (tnp)-primed memory cells localized in recipient lymph nodes draining a footpad injection of tnp-hemocyanin (tnp-klh) in greater numbers than in contralateral nodes draining a p-azobenzenearsonate-coupled klh injection. such hapten-specific, unilateral memory b cell localization was still observed in immunosuppressed mice when antigen injections were given as long as 4 days prior to the memory cell transfer. the memory cells could be challenged to f ... | 1981 | 6173238 |
| application of enzyme-linked immunosorbent assay for epidemiological studies of diseases of livestock in the tropics of mexico. | this study was conducted at the centre for research, teaching and extension in tropical livestock (centro de investigación, enseñanza y extensión en ganadería tropical) of the faculty of veterinary medicine of the national autonomous university of mexico. during the latter part of 1986 and throughout 1988 and 1989, the herd of holstein x zebu cattle at the university was tested for igg antibodies to twenty-one viral, bacterial, rickettsial and parasitic agents. antigens prepared from twenty infe ... | 1993 | 8219327 |
| on the inactivation of brucella abortus in naturally contaminated milk by commercial pasteurisation procedures. | following concern about the recent publication indicating the possible ability of brucella abortus to survive commercial pasteurisation of naturally contaminated milk, such milk was subjected to biological (bt), serological and bacteriological tests. the raw milk was brucella milk ring test positive and biotype i was isolated from 4/5 bt guinea pigs, the one tested being seropositive to the rose bengal test, and with serum agglutination and complement fixation titres of 160 and 36 international ... | 1982 | 6820666 |
| cross-reactivity of haemophilus somnus antibody in agglutination and complement fixation tests and in the enzyme-linked immunosorbent assay. | the specificity and sensitivity of agglutination, complement fixation, and enzyme-linked immunosorbent assay (elisa) procedures in the detection of antibodies to haemophilus somnus was investigated. h. somnus rabbit immune sera were found to agglutinate pasteurella multocida, staphylococcus aureus, and haemophilus agni and, in some instances, also pasteurella haemolytica, salmonella dublin, streptococcus agalactiae, and corynebacterium pyogenes. in complement fixation tests with saline extracts ... | 1983 | 6841584 |
| [barbital buffer in tablets as a solvent for complement fixation test (cft)]. | the purpose of this paper was to develop a tableting technology of barbital buffer as a standard diluent easy in use for complement fixation test (cft). as starting point, barbital (veronal) buffer after alton et al. [1] was assumed. among 4 solubilizers polyvinyl pyrrolidone (pvp 10 000) was chosen, which provided a good solubility of the barbital in distilled water at 20 degrees c and it affected favourably the complement activity (c'). pvp 10 000 added to the components of barbital buffer at ... | 1982 | 7167394 |
| latent carriers of brucellosis. | of 272 heifers subjected to a brucellosis anamnestic test following one injection of k45/20a vaccine, 53 (19%) gave a positive result to the anti-bovine globulin test and 21 (8%) gave a complement fixation test titre at 1/16 or more. three heifers (1%) gave a positive serum agglutination test (sat) titre (60 iu or more) 12 weeks after the second injection of k45/20a vaccine. one of the three aborted at seven months' gestation (brucella abortus culture positive), another had an sat level of 100 i ... | 1980 | 7189074 |
| serological response of cattle after vaccination and challenge with brucella abortus. | new and currently used serological procedures were evaluated using sera from cattle that were challenged with b. abortus s544 (s544) after vaccination with either b. abortus s19 (s19) or b. abortus 45/20 (s45/20) as calves or adults. in animals vaccinated with s19, titres to the indirect haemolysis test (ihlt) rose more slowly, declined more rapidly and involved fewer animals than did titres to the complement fixation test (cft). in animals vaccinated with s45/20 the rough antigen complement fix ... | 1982 | 6810533 |
| isolation of brucella abortus from supramammary lymph nodes of cattle from infected herds vaccinated with low dose strain 19. | | 1984 | 6442860 |
| a clinical and immunopathological study of brucella abortus strain 19--induced arthritis in cattle. | from a series of 11 calves presenting with a clinical unilateral gonitis (inflammation of the stifle), six were subjected to serological examination for titres against brucella abortus and to subsequent post mortem examination. tissues from five calves studied showed similar clinical and radiological features with post mortem examination confirming the presence of characteristic arthritic lesions in the affected stifle and other joints. the immune studies indicated the presence of b abortus stra ... | 1980 | 6776681 |
| characteristics of togo strains of brucella abortus from cattle. | thirty togolese strains of brucella from cattle were classified as members of b. abortus biotype 3 by the recommended methods of the subcommittee on taxonomy of this genus. however two unusual characteristics distinguish the togolese strains from the main group of b. abortus: their mean oxidative profile altered on four of the conventional substrates (l-asparagine, l-arabinose, d-galactose and d-xylose) and their very slow growth on usual media. these two original characteristics are discussed f ... | 1982 | 6820250 |
| outbreak of brucellosis at a south-australian abattoir. 2. epidemiological investigations. | the outbreak of human brucellosis among employees of a large south australian abattoir described previously coincided with an increase in the number of cattle showing a positive serological reaction for brucella abortus being slaughtered. comparisons showed that two other abattoirs in the area were slaughtering larger numbers of such cattle, but no cases of human brucellosis were diagnosed there. this suggested an additional risk at the abattoir concerned. all infected men had been employed in a ... | 1981 | 7334991 |
| vaccination of heifers with a reduced dose of brucella abortus strain 19 vaccine before first mating. | ten jersey heifers aged 14 to 23 months were vaccinated with 2.25 x 10(8) cells of living brucella abortus strain 19 vaccine. they and 10 similar non-vaccinated heifers were subsequently mated and when about 6 months pregnant were challenged by the conjunctival application of a virulent culture of b. abortus. the serological responses to vaccination was much less than is usually seen following vaccination with the normal dose of strain 19, especially when the indirect haemolysis test was used. a ... | 1981 | 7340776 |
| brucellosis in moose (alces alces). a serological survey in an open range cattle area of north central british columbia recently infected with bovine brucellosis. | a serological survey for brucella abortus antibodies in mature cow moose (alces alces) was made in an area of northcentral british columbia which recently had been heavily infected with bovine brucellosis and in which there was considerable intermixing of moose and range cattle. no evidence of brucella infection was found in the moose tested and it was concluded that they were probably not of great significance in the epidemiology of bovine brucellosis in the study area and were therefore unlike ... | 1980 | 7363258 |
| [use of the b19 strain in the prevention of bovine brucellosis in northern cameroon. study of effect of the dose on seroconversion rate and duration in female zebu]. | four herds of zebus from northern cameroon totalling 136 animals were vaccinated subcutaneously with the following doses of brucella abortus strain 19: 5 x 10(9) colony-forming units (cfu), 10(9) cfu, 5 x 10(8) cfu and 10(7) cfu. twenty-eight days after vaccination, the following seroconversion rates were observed respectively: 97.4, 96.2 84.2 and 73.3%. of the 52 animals which could be tested subsequently including 39 over one year old on the vaccination day, only one showed antibodies 6 months ... | 1995 | 7569229 |
| brucella abortus serology in cattle naturally infected with escherichia coli o157.h7. | | 1994 | 7831743 |
| seroprevalences of brucellosis, q-fever and toxoplasmosis in slaughter livestock in trinidad. | serum samples obtained from livestock (cattle, chickens, pigs, sheep, goats and water buffaloes) slaughtered at various slaughter houses in trinidad were screened for agglutinins to three zoonosis causing pathogens. of a total of 751 sera tested, 2 (0.3%) originating from chickens were positive for brucella abortus agglutinins using the rose bengal test (rbt), but both were negative by the tube serum agglutination test (sat). thirty-six (4.8%) of 749 sera were positive for coxiella burnetii aggl ... | 1996 | 8881416 |
| development and validation of an indirect enzyme immunoassay for detection of antibody to brucella abortus in milk. | an indirect enzyme immunoassay for detection of antibody to brucella abortus in bovine milk was developed and validated using 6238 milk samples from canadian herds (brucellosis free) and 202 samples from herds infected with b. abortus (from argentina and chile). the assay utilized lipopolysaccharide as the antigen, immobilized on the polystyrene matrix, whole milk to test and a mouse monoclonal antibody, specific for an epitope of bovine igg1, conjugated with horseradish peroxidase. the sensitiv ... | 1996 | 8914260 |
| cloning, characterization and construction of htra and htra-like mutants of brucella abortus and their survival in balb/c mice. | a genomic library of brucella abortus s2308 was screened for expression of recombinant proteins recognized by sera from mice and from cattle infected with b. abortus. a positive clone, ba1, expressing a 50 kda peptide was recognized by both sera. plasmid pba1, isolated from ba1, was shown by restriction enzyme digestion to possess a 1.9 kb insert. the nucleotide sequence of the pba1 insert revealed an open reading frame with of 1539 bases with a coding capacity of 513 amino acids and a predicted ... | 1994 | 7861951 |
| the brucella abortus vaccine strain b19 carries a deletion in the erythritol catabolic genes. | brucella abortus b19, an avirulent strain obtained by spontaneous mutation, is used worldwide as a vaccine for the control of bovine brucellosis. b19 differs from other b. abortus strains in its sensitivity to erythritol. we took advantage of a previously obtained erythritol sensitive tn5 insertion mutant of b. abortus 2308 to clone the chromosomal region containing erythritol catabolic genes from this representative pathogenic strain and from the vaccine strain b19. physical mapping with restri ... | 1994 | 7926690 |
| safety and immunogenicity of brucella abortus strain rb51 vaccine in pregnant cattle. | to determine the safety and immunogenicity of brucella abortus strain rb51 as a vaccine in pregnant cattle. | 1997 | 9140553 |
| evidence for genetic control of vaccine-induced antibody responses in cattle. | the purpose of our study was to identify evidence for genetic control of immune responses in cattle. to address this question, we evaluated the variation of antibody responses induced by vaccination with brucella abortus strain 19, a live attenuated bacterial vaccine, in large half-sibling families. the data were analyzed using a parametric statistical model that incorporated the effects of sire, bovine major histocompatibility complex (bola) types and parameters related to the experimental desi ... | 1996 | 9157685 |
| intracellular survival of brucella abortus, mycobacterium bovis bcg, salmonella dublin, and salmonella typhimurium in macrophages from cattle genetically resistant to brucella abortus. | peripheral blood monocyte-derived macrophages were obtained from a herd of cows selected, bred, and confirmed as resistant or susceptible by in vivo challenge of brucella abortus strain 2308. the ability to control in vitro intracellular bacterial replication of b. abortus strain 2308, mycobacterium bovis bacillus calmette-guerin (bcg) montreal strain 9003, salmonella dublin strain 5631, and salmonella typhimurium strain 14028 was evaluated in a bactericidal assay. the macrophages from resistant ... | 1996 | 9157686 |
| mechanisms of binding of brucella abortus to mononuclear phagocytes from cows naturally resistant or susceptible to brucellosis. | during the course of bovine brucellosis, brucella abortus adheres to and infects cells of the mononuclear phagocyte system. potential mechanisms of binding, as measured by numbers of phagocytosed bacteria, were studied in two populations of cattle genetically resistant (r) or susceptible (s) to infection with b. abortus. live b. abortus gained entry into cultured bovine macrophages without organism-specific opsonization. bacterial entry into macrophages from r was inhibited by the peptide rgds, ... | 1994 | 7941309 |
| inhibition of complement-mediated killing of brucella abortus by fluid-phase immunoglobulins. | bovine immunoglobulin preparations from normal serum and from sera containing antibodies against brucella abortus interfered with the brucellacidal action of bovine serum, whereas unfractionated normal serum and antisera were not inhibitory. the inhibitory property of immunoglobulin appeared to be attributable to some anticomplementary property because it also interfered with serum-mediated hemolysis of antibody-coated erythrocytes. the supernatant phase obtained after ultracentrifugation of bov ... | 1994 | 7944019 |
| [epidemiology of bovine brucellosis in mali: serologic investigation and initial isolation of strains of brucella abortus]. | the authors report the results of an epidemiological survey of bovine brucellosis in mali, based on a relatively representative sample of 1,000 serum samples from 236 herds. the prevalence of infection in the herds, established by indirect enzyme-linked immunosorbent assay (elisa) was 53% +/- 6.4. the proportion of animals infected was 23.3% +/- 2.5, falling to 22% when compared with the basic serum pool of 9,466 samples. this rate was relatively high in stationary herds in the semi-arid, sub-hu ... | 1994 | 7949352 |
| field study of vaccination of cattle with brucella abortus strains rb51 and 19 under high and low disease prevalence. | to assess humoral and protective immunity in cattle vaccinated by 12 months with brucella abortus vaccine strains rb51 and 19 under field conditions of high and low brucellosis prevalence. | 1998 | 9706206 |
| comparison of particle concentration fluorescence immunoassay to card and complement fixation tests using isolation of brucella abortus as the standard. | serologic test data and bacteriologic culture results from 816 cattle were evaluated. brucella abortus (field strain or strain 19) was isolated from 27.3% of the cows. results of the card test, particle concentration fluorescence immunoassay (pcfia), and complement fixation (cf) test were compared. antibody titers were directly associated with b. abortus isolation, with the majority (64.7%) of field strain isolations having a pcfia value of < or = 0.300 and a cf test result of at least 1+ at a 1 ... | 1994 | 8068749 |
| [evaluation of epidemiologic surveillance of human brucellosis-- occupational disease overcome in the midst of animals]. | national eradication programme of bovine brucellosis in poland has reduced prevalence below 0.5%. the last cow-abortion incidence caused by brucella abortus bovis was recognized in 1982. the registered cases of human brucellosis (610 during 1986-1992) represent chronic occupational infections in man acquired earlier, when bovine brucellosis was common in poland. actually, some of victims show no serological evidence of infection. present small risk of infection permits to stop or to limit (only ... | 1993 | 8171199 |
| a serological survey of bovine brucellosis in the molopo district of bophuthatswana. | a serological investigation was carried out in the molopo district of bophuthatswana to determine the prevalence of brucellosis titre in heifers and cows (n = 9,675) over 24 months of age, which were grazing on communal tribal and privately owned land. the rose bengal, micro serum agglutination and complement fixation (cft) tests were used. two hundred and two (2.1%) animals tested positive with a cft titre of 30 (iu ml-1) or higher. factors that could have contributed to the relatively low prev ... | 1993 | 8176693 |
| immune responses and protection against infection and abortion in cattle experimentally vaccinated with mutant strains of brucella abortus. | twenty-four 10-month-old polled hereford heifers were inoculated sc with live cells of one of the following strains of brucella abortus: s19 delta 31k (n = 4), s19 delta sod (n = 4), rb51 (n = 4), and strain 19 (n = 6); controls (n = 6) were given saline solution. heifers given the deletion mutants s19 delta 31k and s19 delta sod, and those given strain 19 developed antibody responses to b abortus and cutaneous reactions to brucellin. heifers given strain rb51 did not develop antibodies that rea ... | 1993 | 8250382 |
| [latency of brucella abortus causes problems in oriented control: a review]. | this review on brucella abortus in cattle covers the pathogenesis, the epidemiology and the diagnostics of brucellosis. emphasis is given to the presence of latent infections in young stock. calves infected by b. abortus in utero or after ingestion of infected milk may acquire a persistent infection. these animals might present a significant problem in brucellosis control and eradication schemes, since they are difficult to detect by the usual serological tests as they remain negative until near ... | 1993 | 8256248 |
| brucellosis: imported and laboratory-acquired cases, and an overview of treatment trials. | following the successful eradication of brucella abortus infection in cattle, human brucellosis in england and wales has become an uncommon imported disease. culture of the organism presents a major laboratory hazard, and difficulties in identification may occur using a biochemical test-strip method. an overview of recent treatment trials of brucellosis indicates that regimens combining streptomycin and doxycycline are associated with a higher success rate (judged by the frequency of treatment f ... | 1993 | 8337710 |
| comparative analysis of using mtt and xtt in colorimetric assays for quantitating bovine neutrophil bactericidal activity. | two different tetrazolium compounds were compared for use in a colorimetric assay for quantitating bovine neutrophil bactericidal activity against staphylococcus aureus, escherichia coli, listeria monocytogenes, and brucella abortus. the tetrazolium compounds tested included 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (mtt) and sodium 3,3'-[1[(phenylamino)carbonyl]-3,4- tetrazolium]-bis(4-methoxy-6-nitro) benzene sulfonic acid hydrate (xtt). the mtt and xtt colorimetric bacteric ... | 1993 | 8423367 |
| identification of an is711 element interrupting the wboa gene of brucella abortus vaccine strain rb51 and a pcr assay to distinguish strain rb51 from other brucella species and strains. | brucella abortus vaccine strain rb51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. the genetic mutations that are responsible for the roughness and the attenuation of strain rb51 have not been identified until now. also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain rb51 from its parent strain 2308 is available. in the present study, we demonstrate that the wboa gene encoding a glycosyltransferas ... | 1999 | 10473532 |
| single-step pcr for detection of brucella spp. from blood and milk of infected animals. | a versatile method for the extraction of brucella dna and pcr are presented as reliable tools for the detection of brucella spp. from body fluids of infected animals. two oligonucleotides homologous to regions of the gene encoding for an outer membrane protein (omp-2) were designed to detect the pathogen from milk and/or blood of infected goats, bovines, and human patients. the sensitivity of our test and its ability to detect the pathogen in samples from the field reveal a promising advance in ... | 1995 | 8586678 |
| [the epidemiological characteristics of brucellosis in the region of the northern caucasus]. | at present, the northern caucus region is the most unfavourable brucellosis area of the russian federation where 43.5% of the total russian incidence of this infection was recorded in 1993. over the past years (1988-1993) there was a highly unsteady-state improvement of the epidemiological situation, however, the rates of human morbidity with this infection greatly lag behind those in russia. the active foci of small cattle brucellosis as the leading source of human contamination with brucellosi ... | 1995 | 8587517 |
| lymphocyte proliferation in response to brucella abortus rb51 and 2308 proteins in rb51-vaccinated or 2308-infected cattle. | cattle vaccinated with brucella abortus strain rb51 (srb51) or infected with strain 2308 (s2308) had lymph node lymphocytes which proliferated most when incubated with 32-, 27-, 18-, or <18-kda proteins of either srb51 or s2308. some s2308-infected cattle but no srb51-vaccinated cattle had lymphocytes which proliferated in response to 80- and 49-kda proteins of srb51 and s2308. these results suggest that cattle vaccinated with srb51 or infected with s2308 have lymphocytes which proliferate in re ... | 1996 | 8641749 |
| brucella abortus strain rb51 as a vector for heterologous protein expression and induction of specific th1 type immune responses. | brucella abortus strain rb51 is a stable, rough, attenuated mutant widely used as a live vaccine for bovine brucellosis. our ultimate goal is to develop strain rb51 as a preferential vector for the delivery of protective antigens of other intracellular pathogens to which the induction of a strong th1 type of immune response is needed for effective protection. as a first step in that direction, we studied the expression of a foreign reporter protein, beta-galactosidase of escherichia coli, and th ... | 2000 | 10816476 |
| brucella abortus differs in the multiplication within bovine chorioallantoic membrane explants from early and late gestation. | the ability of brucella to infect and grow within extraplacentomal chorioallantoic explants (cams) derived from early and late gestational cattle was compared. following inoculation of cams with equal numbers of strain 2308 b. abortus, the infectivity was approximately the same in cams from both ages, however, bacterial replication was significantly greater in late gestational cams than in early gestational cams. co-culture of both early and late gestation cams or culture of both types of cams i ... | 1996 | 8654046 |
| serological response of cattle to brucella allergen after repeated intradermal applications of this allergen. | a study was conducted to determine whether an allergen that has been prepared from a mucoid strain of brucella abortus triggers a serum antibody response that interferes with the interpretation of serologic tests results. fifteen cattle seronegative for brucella antigen were tested with the sdth test several times. blood samples were collected weekly and tested with the serum agglutination test and complement fixation test. results show that some cattle tested seronegative after each of the sdth ... | 1996 | 8701573 |
| a multiplex approach to molecular detection of brucella abortus and/or mycobacterium bovis infection in cattle. | a multiplex amplification and detection platform for the diagnosis of mycobacterium bovis and brucella abortus infection simultaneously in bovine milk and nasal secretions was developed. this system (designated the bovine pathogen detection assay [bpda]-pcr) consists of duplex amplification of species-specific targets (a region of the bcsp31k gene of b. abortus and a repeat-sequence region in the hsp65 gene of m. bovis, respectively). this is followed by a solid-phase probe capture hybridization ... | 2000 | 10878051 |
| cloning and kinetics of expression of brucella abortus heat shock proteins by baculovirus recombinants. | in an effort to develop genetically engineered brucella abortus (ba) vaccines, the genes encoding heat shock proteins (hsps) groel, groes, and htra were cloned and expressed in the bac-to-bac baculovirus system, and the kinetics of protein expression were analyzed using various insect cell lines in suspension cultures, different cell densities in suspension cultures, multiplicities of infection and recombinant virus replication times. trichoplusia ni cells expressed only ba htra, but spodoptera ... | 2000 | 10889410 |
| attempts to use thiabendazole to improve the immune response in dexamethasone-treated or stressed cattle. | thiabendazole was evaluated in two separate experiments for its ability to enhance the immune response in dexamethasone-treated or stressed cattle. in the first experiment the cattle received either no drug treatment (controls), dexamethasone intramuscularly (im), or dexamethasone im plus thiabendazole orally. all animals were inoculated with heat-killed brucella abortus strain 19, equine ferritin, tetanus toxoid, and live corynebacterium equi at the time dexamethasone therapy was initiated. dex ... | 1984 | 6519958 |
| cellular and humoral responses of brucella abortus-infected bovine fetuses. | fifty-nine bovine fetuses naturally and experimentally infected with brucella abortus were studied. lymphoid hyperplasia in multiple lymph nodes, lymphoid depletion in the thymic cortex, adrenal cortical hyperplasia, and disseminated inflammatory foci composed mainly of large mononuclear leukocytes were present in infected fetuses. histopathologic changes in naturally infected fetuses were indistinguishable from those infected fetuses inoculated in utero. fetuses inoculated with 1.0 x 10(3) to 1 ... | 1984 | 6538768 |
| a comparative study of elisa and other methods for the detection of brucella antibodies in bovine sera. | enzyme-linked immunosorbent assay (elisa), using beta-galactosidase and a fluorigenic substrate, was used for the detection of antibodies to brucella abortus in bovine sera. among 677 animals from 9 brucellosis-free herds, none reacted in the elisa. among 785 animals from 23 brucellosis-infected herds, 336 were positive in elisa, 229 in the slow agglutination test (sat), 185 in the complement fixation test (cft), and 165 in the rose-bengal test (rbt). experimental infections were conducted with ... | 1984 | 6442027 |
| humoral immune response against lipopolysaccharide and cytoplasmic proteins of brucella abortus in cattle vaccinated with b. abortus s19 or experimentally infected with yersinia enterocolitica serotype 0:9. | the humoral immune responses against three different antigens of brucella abortus were monitored by enzyme-linked immunosorbent assay in cattle vaccinated with b. abortus s19 or experimentally infected with yersinia enterocolitica serotype 0:9. immunoglobulin g (igg) and igm responses against (i) b. abortus lipopolysaccharide (lps), (ii) total cytoplasmic proteins depleted of lps (lps-free cyt), and (iii) b. abortus 18-kda cytoplasmic protein were measured. vaccinated animals and yersinia-infect ... | 1996 | 8807216 |
| infection of cattle with brucella abortus biovar 1 isolated from a bison in wood buffalo national park. | an experiment was conducted to determine if cattle could be infected with a strain of brucella abortus biovar 1 isolated from a bison in wood buffalo national park. three pregnant cows inoculated conjunctivally with 5.7 x 10(8) cfu of the bacterium, and their subsequent calves, showed seroconversion on standard serological tests for bovine brucellosis, and large numbers of the bacterium were isolated from numerous tissues at necropsy. a 4th cow that was moved into the pen that previously contain ... | 1996 | 8809394 |
| enhancement of the brucella amos pcr assay for differentiation of brucella abortus vaccine strains s19 and rb51. | because the brucellosis eradication program uses slaughter and quarantine as control measures, it would benefit from faster methods of bacterial identification. distinguishing vaccine strains from strains that cause infections among vaccinated herds in the field is essential. to accomplish this, our pcr-based, species-specific assay (b. j. bricker and s. m. halling, j. clin. microbiol. 32:2660-2666, 1994) was updated to identify brucella abortus vaccine strains s19 and rb51. three new oligonucle ... | 1995 | 7650203 |
| the use of divalent cation chelating agents (edta/egta) to reduce non-specific serum protein interaction in enzyme immunoassay. | an indirect enzyme immunoassay (elisa) for detection of bovine antibody to brucella abortus was modified by the addition of divalent chelating agents to the serum diluent. this addition resulted in an increase in specificity from 96.0% in the regular assay to 99.4% in the modified procedure. of the 15,715 sera initially tested by the indirect elisa, 691 that had given positive reactions were selected for retesting in the indirect elisa with edta/egta added. the buffered plate antigen test (bpat) ... | 1994 | 7701780 |
| immune responses to superoxide dismutase and synthetic peptides of superoxide dismutase in cattle vaccinated with brucella abortus strain 19 or rb51. | antibody and lymph node cell-mediated immune responses to recombinant brucella abortus strain 19 cu-zn superoxide dismutase (rsod) and to three synthetic strain 19 cu-zn sod peptides were measured during 2 to 12 weeks following vaccination of cattle with b. abortus strain 19 or rb51. cattle vaccinated with strain 19 or rb51 did not produce antibody to rsod and to the sod peptides. lymph node cells from cattle vaccinated with strain 19, but not with strain rb51, proliferated when incubated with e ... | 1994 | 7801538 |
| serologic responses of brucella abortus strain 19 calfhood-vaccinated cattle following adult vaccination with strain rb51. | this study was designed to determine if brucella abortus strain rb51, which expresses small amounts of the lipopolysaccharide o side chain, would cause positive responses on brucellosis serologic surveillance tests when given to adult cattle that were vaccinated as calves with b. abortus strain 19. cattle vaccinated as adults with strain rb51 that had been vaccinated as calves with strain 19 (n = 40) had significantly greater antibody titers (p < 0.05) against strain rb51 at 4 and 8 weeks postva ... | 1996 | 8953530 |