Publications

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transplacental transmission of bovine tick-borne pathogens: frequency, co-infections and fatal neonatal anaplasmosis in a region of enzootic stability in the northeast of brazil.bovine tick-borne disease (tbd) constitutes a worldwide group of diseases that result in great losses for dairy and beef cattle. with regard to the epidemiological profile of the diseases, the importance of transplacental transmission is still not very well understood. the aim of this study was to determine the transplacental transmission of tbd agents (anaplasma marginale, babesia bovis and b. bigemina) in a herd of dairy cattle that had been naturally infected in an area of enzootic stability ...201626613663
low genetic diversity of anaplasma marginale in calves in an endemic area for bovine anaplasmosis in the state of são paulo, brazil.major surface protein 1a has been used as a marker for genetic stability in identifying geographical isolates of anaplasma marginale. we conducted a longitudinal study to ascertain the genetic diversity of a. marginale in a dairy cattle herd with a history of clinical anaplasmosis. a total of 20 calves were evaluated every 3 months, from birth to 1 year of age. they were evaluated using blood smears, ifat, elisa, and qpcr. additionally, samples positive for the msp1a gene using npcr were sequenc ...201626318264
bovine babesiosis and anaplasmosis complex: diagnosis and evaluation of the risk factors from bahia, brazil.direct diagnoses were made by using - blood smears and nested pcr (npcr) tests on 309 blood samples from crossbred dairy cattle in the municipality of ibicaraí, bahia. from diagnostic blood smear slides, the observed parasitic frequencies were 31.1% for anaplasma marginale and 20.4% for babesia sp. from npcr diagnoses, they were 63% for a. marginale, 34% for babesia bigemina and 20.4% for babesia bovis. there were significant differences (p <0.01) between the two diagnostic methods (npcr and blo ...201625271452
a comparison of six primer sets for detection of trypanosoma evansi by polymerase chain reaction in rodents and thai livestock.to face the worldwide threat of surra caused by trypanosoma evansi, international organizations have stressed the need to evaluate and standardize diagnostic tools. pcr detection of t. evansi has known a great expansion during the last 20 years, but primer sets are often insufficiently assessed and compared. in this work, we compared the performances of six primer pairs-tbr1/2 (masiga et al., 1992), esag6/7 (holland et al., 2001a, b), tepan1/2 (panyim et al., 1993), pmutec f/r (wuyts et al., 199 ...201020466489
development and laboratory evaluation of a lateral flow device (lfd) for the serodiagnosis of theileria annulata infection.several dna-based and serological tests have been established for the detection of theileria annulata infection, including polymerase chain reaction, reverse line blot and loop-mediated isothermal amplification, indirect enzyme-linked immunosorbent assay (elisa), and competitive elisa. in this study, we have applied knowledge from the development and application of a recombinant protein-based indirect elisa and competitive elisa to establish a rapid test for point-of-care diagnosis of t. annulat ...201020680339
[transcription of genes of membrane proteins of brazilians isolates of anaplasma marginale].this work shows the transcription profile of membrane protein genes in three brazilian isolates of anaplasma marginale (rio grande do norte, pernambuco-zona da mata, and pernambuco-sertão). rna was purified from cattle blood experimentally-infected with the three isolates of a. marginale. after reverse transcription, genes omp1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, and 14; opag1-3; virb3, 9, and 10; am097, 197, 254, 854, and 956 were amplified by pcr, with specific primers. transcripts were dete ...200718078602
identification of a novel anaplasma marginale appendage-associated protein that localizes with actin filaments during intraerythrocytic infection.the rickettsial pathogen anaplasma marginale assembles an actin filament bundle during intracellular infection. unlike other bacterial pathogens that generate actin filament tails, a. marginale infects mature erythrocytes, and the f-actin appendages are assembled on the cytoplasmic surface of a vacuole containing several organisms. to identify a. marginale molecules associated with these filaments, two complementary approaches were used: matrix-assisted laser desorption ionization-time-of-flight ...200415557651
molecular epidemiological survey and genetic characterization of anaplasma species in mongolian livestock.anaplasma species are obligate intracellular rickettsial pathogens that cause great economic loss to the animal industry. few studies on anaplasma infections in mongolian livestock have been conducted. this study examined the prevalence of anaplasma marginale, anaplasma ovis, anaplasma phagocytophilum, and anaplasma bovis by polymerase chain reaction assay in 928 blood samples collected from native cattle and dairy cattle (bos taurus), yaks (bos grunniens), sheep (ovis aries), and goats (capra a ...201728678004
conservation of a gene conversion mechanism in two distantly related paralogues of anaplasma marginale.anaplasmataceae, the causative agents of anaplasmosis and ehrlichiosis, persist in the bloodstream of their mammalian hosts, allowing acquisition and transmission by tick vectors. anaplasma marginale establishes persistent infection characterized by sequential cycles of rickettsaemia in which new antigenic variants emerge. the two most immunodominant outer membrane proteins, msp2 and msp3, are paralogues, each encoded by a distinct family of related genes. this study demonstrates that, although ...200312535066
a novel ehrlichia genotype detected in naturally infected cattle in north america.during a research investigation to determine if cattle from british columbia (bc), canada were infected with anaplasma marginale or other related rickettsial blood parasites, a novel ehrlichia genotype was revealed. blood from seven bc source cattle was bioassayed by intravenous inoculation into naïve splenectomised calves. additional splenectomised calves were used as uninoculated negative control or a. marginale-inoculated positive control. newly designed sets of primers specific for the msp5 ...201020663613
Acute phase response in cattle infected with Anaplasma marginale.This study was undertaken to evaluate the acute phase responses via the assessment of the concentration of serum sialic acids (total, lipid bound and protein bound), inflammatory mediators (IFN-? and TNF-a) and acute phase proteins (Hp and SAA) in 20 adult crossbred cattle naturally infected by Anaplasma marginale. The infected animals were divided into 2 subgroups on the basis of parasitemia rate (<20% and >20%). Also, as a control group, 10 clinically healthy cattle from the same farms were sa ...201121940118
development of a multiplex pcr assay for simultaneous detection of theileria annulata, babesia bovis and anaplasma marginale in cattle.tropical theileriosis, bovine babesiosis and anaplasmosis are tick-borne protozoan diseases that impose serious constraints on the health and productivity of domestic cattle in tropical and sub-tropical regions of the world. a common feature of these diseases is that, following recovery from primary infection, animals become persistent carriers of the pathogen and continue to play a critical role in disease epidemiology, acting as reservoirs of infection. this study describes development and eva ...201223183165
detecting and differentiating theileria sergenti and theileria sinensis in cattle and yaks by pcr based on major piroplasm surface protein (mpsp).theileria sergenti and theileria sinensis are closely related members of benign theileria species found in cattle and yaks in china. they are morphologically indistinguishable. a polymerase chain reaction (pcr) targeting major piroplasm surface protein of t. sergenti and t. sinensis was developed in this study. the newly developed oligonucleotide primer set was able to specifically amplify the dna of t. sinensis and in conjunction with primers for t. sergenti and these two species could be detec ...201020685208
haemoparasite prevalence and theileria parva strain diversity in cape buffalo (syncerus caffer) in uganda.cape buffalo (syncerus caffer) are considered to be an important reservoir for various tick-borne haemoparasites of veterinary importance. in this study we have compared the haemoparasite carrier prevalence in buffalo from four geographically isolated national parks in uganda [lake mburo national park (lmnp), queen elizabeth national park (qenp), murchison falls national park (mfnp) and kidepo valley national park (kvnp)]. differences were seen in haemoparasite prevalence in buffalo from the fou ...201021074945
validation and field assessment of a rapid lateral flow assay for detection of bovine antibody to anaplasma marginale.the lateral flow assay (lfa) is a rapid diagnostic test which may be performed under most conditions and is especially useful for field applications. this type of assay was applied to the detection of antibody to bovine anaplasma marginale using sera from endemic areas and from areas which have been free from infection for more than 25 years. briefly, the test uses recombinant a. marginale major surface protein 5 peptide (msp5), immobilized on a cellulose acetate membrane. a serum sample is adde ...200919591044
risk factors for anaplasmosis in dairy cows during the peripartum.anaplasma marginale is endemic in tropical and subtropical areas around the world. some studies have suggested that cows during peripartum may present a transient immunosuppression state and development of clinical signs of anaplasmosis. the aim of this study was to investigate the relationship between some risk factors and the seroprevalence of a. marginale in dairy cows during peripartum in rio de janeiro, brazil. the risk factors analyzed in association with the prevalence of antibodies again ...201424307390
epidemiological analysis of tick-borne diseases in zambia.tick-borne diseases are a constraint to livestock production in many developing countries as they cause high morbidity and mortality, which results in decreased production of meat, milk and other livestock by-products. the most important tick-borne diseases of livestock in sub-saharan africa are east coast fever (caused by theileria parva), babesiosis (caused by babesia bigemina and b. bovis), anaplasmosis (caused by anaplasma marginale) and heartwater (caused by ehrlichia ruminantium). despite ...201021106294
association of bola-drb3 alleles with tick-borne disease tolerance in dairy cattle in a tropical environment.tick-borne disease is one of the most harmful tropical diseases in dairy production. selection of dairy cows for tolerance to tick-borne disease is a challenging concept for dairy breeders in the tropics. the objectives of this study were (1) to detect specific tick-borne pathogen in cattle of different genetics and (2) to examine the polymorphisms of drb3.2 alleles in thai dairy cattle and find the allelic association with tick-borne disease tolerance. specific primers to anaplasma marginale (a ...201323571121
prevalence and haemato-biochemical profile of anaplasma marginale infection in dairy animals of punjab (india).to do the systematic comparison of prevalence of anaplasmosis by pcr and giemsa stained thin blood smear (gstbs) based parasitological assays in dairy cattle of punjab, which has not been reported yet. to analyse the haematobiochemical alterations in infected animals to arrive at the conclusion regarding the pathogenicity induced by anaplasma marginale (a. marginale) in latent and patent infection.201323339917
genetic diversity and molecular phylogeny of anaplasma marginale isolates from minas gerais, brazil.anaplasma marginale (rickettsiales: anaplasmataceae), a tick-borne pathogen of cattle, is endemic in tropical and subtropical regions of the world, and many isolates of a. marginale may occur in a given geographic area. phylogenetic relationships have been reported for a. marginale isolates from the us using gene and protein sequences of msp1a and msp4. these studies demonstrated that msp4 sequences, but not msp1a dna or protein sequences, provide phylogeographic information and also that msp1a ...200415135871
immunogenicity of anaplasma marginale type iv secretion system proteins in a protective outer membrane vaccine.rickettsial pathogens in the genera anaplasma and ehrlichia cause acute infection in immunologically naive hosts and are major causes of tick-borne disease in animals and humans. immunization with purified outer membranes induces protection against acute anaplasma marginale infection and disease, and a proteomic and genomic approach recently identified 21 proteins within the outer membrane immunogen in addition to the well-characterized major surface proteins msp1 to msp5. among the newly descri ...200717339347
differential expression of genes in salivary glands of male rhipicephalus (boophilus)microplus in response to infection with anaplasma marginale.bovine anaplasmosis, caused by the rickettsial tick-borne pathogen anaplasma marginale (rickettsiales: anaplasmataceae), is vectored by rhipicephalus (boophilus)microplus in many tropical and subtropical regions of the world. a. marginale undergoes a complex developmental cycle in ticks which results in infection of salivary glands from where the pathogen is transmitted to cattle. in previous studies, we reported modification of gene expression in dermacentor variabilis and cultured ixodes scapu ...201020298599
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