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belgrade virus, a cause of hemorrhagic fever with renal syndrome in the balkans, is closely related to dobrava virus of field mice.belgrade virus is a recently described hantavirus that causes severe hemorrhagic fever with renal syndrome (hfrs) in people living in various parts of the balkan peninsula. nucleotide sequencing of the g2-encoding region in the medium (m) segment of the viral genome, reverse transcribed and amplified by the polymerase chain reaction, revealed the belgrade virus to be substantially different from hantaan virus and other major serotypes of hantavirus but identical to dobrava virus, a virus isolate ...19938102632
puumala and dobrava viruses cause hemorrhagic fever with renal syndrome in bosnia-herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera.hantavirus infection was diagnosed serologically by mu-capture igm and igg elisas in hemorrhagic fever with renal syndrome (hfrs) patients admitted to tuzla hospital, bosnia-herzegovina. the results indicated that more than one hantavirus caused the outbreak. to address the question of which hantavirus serotypes were involved, sequentially drawn sera were analyzed by focus reduction neutralization test (frnt) for antibodies against puumala, hantaan, dobrava, and seoul hantaviruses. the data reve ...19979298732
dobrava hantavirus causes hemorrhagic fever with renal syndrome in central europe and is carried by two different apodemus mice species.in central europe, hemorrhagic fevers with renal syndrome (hfrs) in humans are caused by the hantavirus species puumala (transmitted by voles) and a second, hantaan-related species (transmitted by mice). the second virus could be identified as dobrava virus. to date, 19 clinical cases of dobrava infection have been found in germany and slovakia. all patients exhibited a mild/moderate clinical course and no case fatality occurred. screening for infected rodents revealed that the striped field mou ...200111170053
isolation of dobrava virus from apodemus flavicollis in greece.dobrava virus (dobv) carried by apodemus flavicollis is the causative agent of severe hemorrhagic fever with renal syndrome (hfrs). dobv was isolated from an a. flavicollis mouse trapped in northeastern greece. this is the third dobv cell culture isolate in the world, clustering together with other greek dobv sequences from hfrs patients and rodents.200111376073
hantavirus infections in greece--an update.hantaviruses are emerging viruses spread worldwide and cause hemorrhagic fever with renal syndrome (hfrs) or hantavirus pulmonary syndrome (hps). two pathogenic hantaviruses are present in greece: dobrava/belgrade virus (dobv) and puumala virus (puuv). the predominant one is dobv, carried in greece by the rodent apodemus flavicollis. most human infections are sporadic, but epidemic outbreaks were also described. the severity of the disease ranges from a mild or moderate form to severe and fatal ...200111599695
genetic interaction between distinct dobrava hantavirus subtypes in apodemus agrarius and a. flavicollis in nature.dobrava virus (dobv) occurs in two different rodent species, apodemus flavicollis (dobv-af) and a. agrarius (dobv-aa). we sequenced the s and m genomic segments from sympatric dobv-af and dobv-aa strains which fell into two distinct genetic lineages. molecular phylogenetic analyses gave evidence for genetic reassortment between s and m segments of dobv-af and dobv-aa and indicated homologous recombination events in dobv evolution. dobv-af and dobv-aa are distinct but also subject to genetic exch ...200312477889
central european dobrava hantavirus isolate from a striped field mouse (apodemus agrarius).dobrava virus (dobv) is a hantavirus that causes hemorrhagic fever with renal syndrome (hfrs) in europe. it is hosted by at least two rodent species, apodemus flavicollis and a. agrarius. according to their natural hosts they form the distinct genetic lineages dobv-af and dobv-aa, respectively. we have now established a dobv isolate named slovakia (sk/aa) from an a. agrarius animal captured in slovakia. the complete s and m and partial l segment nucleotide sequences of the new isolate were deter ...200515956394
hantavirus and arenavirus antibody prevalence in rodents and humans in trentino, northern italy.the spatial and temporal distribution of hantavirus and arenavirus antibody-positive wild rodents in trentino, italy, was studied using immunofluorescence assays (ifa) in two long-term sites trapped in 2000-2003, and six other sites trapped in 2002. the overall hantavirus seroprevalence in the bank voles, clethrionomys glareolus (n=229) screened for puumala virus (puuv) antibodies was 0.4%, and that for apodemus flavicollis mice (n=1416) screened for dobrava virus (dobv) antibodies was 0.2%. ant ...200616371172
co-circulation of three pathogenic hantaviruses: puumala, dobrava, and saaremaa in hungary.hantaviruses (bunyaviridae) cause hemorrhagic fever with renal syndrome (hfrs) in eurasia and hantavirus (cardio)pulmonary syndrome (hcps) in the americas. hfrs is caused by hantaan virus (htnv), seoul virus (seov), dobrava virus (dobv), saaremaa virus (saav), and puumala virus (puuv). of those, only htnv is not present in europe. in recent years, hantaviruses, described in other parts of europe, were also detected at various locations in hungary. to study the genetic properties of hungarian han ...200919856478
hantavirus infection during a stay in a mountain hut in northern slovakia.hantaviruses in europe cause human hemorrhagic fever with renal syndrome (hfrs) with various degree of severity. the most severe form is caused by the dobrava/belgrade virus (dobv), associated with the rodent apodemus flavicollis. during the last decade cases of infection caused by dobv have been reported in central europe. the present study is a report on two czech patients with severe hfrs who were infected during their stay in a mountain hut in northern slovakia. the two patients, combined wi ...201121264871
dobrava-belgrade virus in apodemus flavicollis and a. uralensis mice, turkey.in 2009, human dobrava-belgrade virus (dobv) infections were reported on the black sea coast of turkey. serologic and molecular studies of potential rodent reservoirs demonstrated dobv infections in apodemus flavicollis and a. uralensis mice. phylogenetic analysis of dobv strains showed their similarity to a. flavicollis mice-borne dobv in greece, slovenia, and slovakia.201424377763
dobrava virus carried by the yellow-necked field mouse apodemus flavicollis, causing hemorrhagic fever with renal syndrome in romania.hemorrhagic fever with renal syndrome (hfrs) has been confirmed by serological methods during recent years in romania. in the present study, focus-reduction neutralization tests (frnt) confirmed dobrava hantavirus (dobv) as the causative agent in some hfrs cases, but could not distinguish between dobv and saaremaa virus (saav) infections in other cases. dobv was detected by a dobv-specific taqman assay in sera of nine patients out of 22 tested. partial sequences of the m genomic segment of dobv ...201424746107
genetic detection of dobrava-belgrade hantavirus in the edible dormouse (glis glis) in central serbia.hantaviruses are endemic in the balkans, particularly in serbia, where sporadic cases and/or outbreaks of hantaviral human disease have been reported repeatedly, and evidenced serologically. here, we present genetic detection of dobrava-belgrade virus (dobv) hantaviral sequences in wild rodents trapped in central serbia. all the animals were pre-screened serologically by indirect immunofluorescence (if) test and only those with a positive finding of hantaviral antigens were further tested by pol ...201524762257
high infection rate of bank voles (myodes glareolus) with puumala virus is associated with a winter outbreak of haemorrhagic fever with renal syndrome in croatia.an outbreak of haemorrhagic fever with renal syndrome (hfrs) started on medvednica mountain near zagreb in january 2012. in order to detect the aetiological agent of the disease in small rodents and to make the link with the human outbreak, rodents were trapped at four different altitudes. using nested rt-pcr, puumala virus (puuv) rna was detected in 41/53 (77·4%) bank voles (myodes glareolus) and dobrava virus (dobv) rna was found in 6/61 (9·8%) yellow-necked mice (apodemus flavicollis). sequen ...201424800636
genetic analysis of dobrava-belgrade virus from western serbia--a newly detected focus in the balkan peninsula.dobrava-belgrade virus (dobv) is a hantavirus species that causes the most severe form of haemorrhagic fever with renal syndrome (hfrs) in europe. dobv has been detected in three apodemus rodents: a. flavicollis, a. agrarius and a. ponticus. these emerging viruses appear throughout the balkan peninsula including serbia as its central part. in this study, we examined the seroprevalence, molecular epidemiology and phylogenetics of dobv from a. flavicollis captured at six serbian localities. furthe ...201524867363
surveillance of hantaviruses in poland: a study of animal reservoirs and human hantavirus disease in subcarpathia.the first cluster of hemorrhagic fever with renal syndrome (hfrs) in poland was identified in 2007 in the subcarpathian region. the natural environment of this area is a key habitat for hantavirus vectors. the animal reservoir of existing human hfrs clusters was studied to assess the occurrence of viruses (including tula virus, puumala virus, and dobrava-belgrade virus) among rodents. we examined 70 suspected human cases with symptoms corresponding to the clinical picture of hfrs. serological an ...201424902039
recent increase in prevalence of antibodies to dobrava-belgrade virus (dobv) in yellow-necked mice in northern italy.dobrava-belgrade virus (dobv) is the most pathogenic hantavirus in europe with a case-fatality rate of up to 12%. to detect changes in risk for humans, the prevalence of antibodies to dobv has been monitored in a population of apodemus flavicollis in the province of trento (northern italy) since 2000, and a sudden increase was observed in 2010. in the 13-year period of this study, 2077 animals were live-trapped and mean hantavirus seroprevalence was 2·7% (s.e. = 0·3%), ranging from 0% (in 2000, ...201526050717
serological survey of hantavirus and flavivirus among wild rodents in central italy.hantaviruses are a group of zoonotic viruses carried by rodents. puumala virus (puuv) and dobrava virus (dobv) are the causative agents of human hantavirus infections in europe. knowledge about hantavirus circulation in italy is very scarce. west nile virus (wnv) and usutu virus (usuv) are emerging neuropathogenic flaviviruses, both endemic in most part of the italian territories. to monitor the circulation of puuv, dobv, wnv, and usuv in natural environment in central italy, we carried out sero ...201728953448
dobrava virus as a new hantavirus: evidenced by comparative sequence analysis.dobrava virus, recently isolated from a yellow-neck mouse (apodemus flavicollis), captured in a northern slovenian village where severe cases of hemorrhagic fever with renal syndrome were recognized, was shown by serology and restriction enzyme digestion of pcr-amplified gene segments to be related to previously recognized hantaviruses. to investigate further the relationship of this new isolate to other hantaviruses, a portion of the medium (m) genome segment of dobrava virus was amplified by p ...19938487037
dobrava-belgrade virus: phylogeny, epidemiology, disease.dobrava-belgrade virus (dobv) is an old world hantavirus that causes hemorrhagic fever with renal syndrome in humans. with a case fatality rate up to 12%, dobv infection is the most life-threatening hantavirus disease in europe. the virus was initially identified in the balkans, but the discovery of new endemic foci have expanded its recognized geographic range. the recent description of novel genetic variants with different degrees of pathogenicity have complicated its taxonomic analysis. the o ...201222659378
distribution and ecology of small mammals in albania.in order to gain an insight into the population dynamics and habitat preferences of rodents in albania, small mammals were collected during 2006-2009 in various districts of the country. of the 15 species of small mammals captured (12 in rodentia and 3 in insectivora order), the yellow-necked mouse (apodemus flavicollis) was the most frequently collected, representing 50% of the 325 small mammals captured, followed by the wood mouse (apodemus sylvaticus) (20.62%). apodemus flavicollis is the pri ...201322963015
multiple co-infections of rodents with hantaviruses, leptospira, and babesia in croatia.hantaviruses, leptospira spp., and babesia spp. are rodent-borne pathogens present worldwide. we studied multiple co-infections of small rodents in croatia with all three pathogens. twenty-eight apodemus flavicollis and 16 myodes glareolus were tested for the presence of hantavirus rna by real-time rt-pcr, leptospira strains by renoculture method and babesia dna by pcr. anti-hantavirus antibodies and anti-leptospira antibodies were detected by serological methods. very high infection rates with ...201222217170
genetic detection of dobrava/belgrade virus in a czech patient with haemorrhagic fever with renal syndrome.in the summer of 2008, a 15-year-old boy was hospitalized in a paediatric intensive care unit in the czech republic. laboratory diagnosis of hantavirus infection was established by serological and molecular methods. sequence and phylogenetic analyses showed that the causative strain was dobrava/belgrade virus, which is genetically closer to strains associated with apodemus flavicollis rodents.201019832712
population dynamics of wild rodents induce stochastic fadeouts of a zoonotic pathogen.stochastic processes play an important role in the infectious disease dynamics of wildlife, especially in species subject to large population oscillations. here, we study the case of a free ranging population of yellow-necked mice (apodemus flavicollis) in northern italy, where circulation of dobrava-belgrade hantavirus (dobv) has been detected intermittently since 2001, until an outbreak emerged in 2010. we analysed the transmission dynamics of the recent outbreak using a computational model th ...201728217934
genetic detection of hantaviruses in rodents, albania.in order to have a first insight into the epidemiology of hantaviruses in albania, 263 small mammals (248 rodents, 15 insectivores) were captured in 352 locations in 29 districts and tested for hantavirus infection. dobrava-belgrade virus (dobv) was detected in 10 of 148 (6.7%) apodemus flavicollis rodents. dobv-positive a. flavicollis were detected in six districts (diber, korce, kolonje, librazhd, pogradec, and vlore). the obtained nucleotide sequences were highly similar to each other and to ...201627249068
the hantaviral load in tissues of naturally infected rodents.hantaviruses cause a lifelong and asymptomatic infection in naturally infected hosts as well as in experimentally infected rodents. understanding the ecology and pathogenesis of hantaviruses requires an interdisciplinary research approach, which links laboratory experiments with results gained from field studies. although several studies report hantavirus persistence and tissue infection patterns for experimentally infected rodents, field data is very limited. for this reason, the aim of our stu ...200919397875
genetic characterization of new dobrava hantavirus isolate from greece.the first complete genome sequence of dobrava hantavirus isolated from yellow-necked mouse apodemus flavicollis trapped in the northeastern greece is described. the s, m, and l segments of the greek isolate of dobrava virus are 1673, 3635, and 6532 nucleotides (nt) long, respectively, and encode the nucleocapsid (n) protein of 429 amino acids (aa), glycoprotein precursor of 1135 aa, and the l protein of 2151 aa. n protein contains three cysteine residues conserved in all known hantaviruses, as w ...200312526053
identification of genetic evidence for dobrava virus spillover in rodents by nested reverse transcription (rt)-pcr and taqman rt-pcr.a survey of 158 rodents caught in the czech republic identified dobrava virus sequences closely related to that of the dobrava virus type strain in apodemus sylvaticus and mus musculus rodents. the identity of a. sylvaticus was unequivocally confirmed by random amplified polymorphic dna analysis. the data seem to indicate hantavirus spillover from apodemus flavicollis to other rodents.200515695684
[puumala and dobrava viruses in the northeastern and central regions of bosnia].bosnia and herzegovina has been known as a highly endemic region for hantavirus infections for more than 50 years. previous studies have shown that at least two different hantaviruses, the murine dobrava (dob) and avricoline puumala (puu) viruses, each carried by a different rodent species, have been circulating in the area. however, there is little information on rodent population density fluctuations in bosnia over the past years as well as on the ratio of puumala to dobrava infection in human ...200315011464
yeast-expressed hantavirus dobrava nucleocapsid protein induces a strong, long-lasting, and highly cross-reactive immune response in mice.in europe, dobrava virus (dobv) carried by the yellow-necked field mouse apodemus flavicollis is one of the hantaviruses that can cause severe hemorrhagic fever with renal syndrome in humans. for several hantaviruses, the nucleocapsid (n) protein has proven to be very immunogenic in humans and rodents and even can protect rodents against a virus challenge. to investigate the immunogenicity of dobv n protein, balb/c and c57bl/6 mice were immunized three times with a dobv recombinant n (rn) protei ...200415018668
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