Publications

rna-seq analyses of blood-induced changes in gene expression in the mosquito vector species, aedes aegypti.	hematophagy is a common trait of insect vectors of disease. extensive genome-wide transcriptional changes occur in mosquitoes after blood meals, and these are related to digestive and reproductive processes, among others. studies of these changes are expected to reveal molecular targets for novel vector control and pathogen transmission-blocking strategies. the mosquito aedes aegypti (diptera, culicidae), a vector of dengue viruses, yellow fever virus (yfv) and chikungunya virus (cv), is the sub ...	2011	21276245
[entomological investigations conducted around ten cases of yellow fever in 2009 in the denguélé sanitary region, côte-d'ivoire.]	in november 2009, ten suspicious cases of yellow fever, including six deaths, were notified in the region of denguélé, in the northwest of côte-d'ivoire. in order to evaluate the extent of yellow fever virus circulation and the risk for local people, a mission of entomological investigation was carried out by the ministry of health and public hygiene of côte-d'ivoire. entomological investigations were conducted in the villages of confirmed cases (banakoro and tron-touba) and the centers of consu ...	2011	21293956
a protocol for collecting and staining hemocytes from the yellow fever mosquito <em>aedes aegypti</em>.	mosquitoes are vectors for a number of disease-causing pathogens such as the yellow fever virus, malaria parasites and filarial worms. laboratories are investigating anti-pathogen components of the innate immune system in disease vector species in the hopes of generating transgenic mosquitoes that are refractory to such pathogens(1, 2). the innate immune system of mosquitoes consists of several lines of defense (3). pathogens that manage to escape the barrier imposed by the epithelium-lined mosq ...	2011	21633325
vector competence of australian mosquitoes for yellow fever virus.	the vector competence of australian mosquitoes for yellow fever virus (yfv) was evaluated. infection and transmission rates in cairns and townsville populations of aedes aegypti and a brisbane strain of ae. notoscriptus were not significantly different from a well-characterized yfv-susceptible strain of ae. aegypti. after exposure to 10⁷·² tissue culture infectious dose (tcid₅₀)/ml of an african strain of yfv, > 70% of ae. aegypti and ae. notoscriptus became infected, and > 50% transmitted the v ...	2011	21896802
development of a sybr green i based rt-pcr assay for yellow fever virus: application in assessment of yfv infection in aedes aegypti.	yellow fever virus (yfv) is an important arboviral pathogen in much of sub-saharan africa and the tropical americas. it is the prototype member of the genus flavivirus and is transmitted primarily by aedes (stegomyia) mosquitoes. the incidence of human infections in endemic areas has risen in recent years. prompt and dependable identification of yfv is a critical component of response to suspect cases.	2012	22264275
yellow fever virus susceptibility of two mosquito vectors from kenya, east africa.	yellow fever is an unpredictable disease of increasing epidemic threat in east africa. aedes (stegomyia) aegypti has never been implicated as a vector in this region and recent outbreaks have involved a newly emerging virus genotype (east african). to better understand the increasing epidemic risk of yellow fever in east africa, this study is the first to investigate the vector competence for an emerging east african virus genotype in kenyan a. aegypti sensu latu (s.l) and a. (stegomyia) simpson ...	2012	22521217
[yellow fever: reemerging in the state of sao paulo, brazil, 2009].	to describe the investigation of a sylvatic yellow fever outbreak in the state of sao paulo and the main control measures undertaken.	2013	24626492
is there a risk of yellow fever virus transmission in south asian countries with hyperendemic dengue?	the fact that yellow fever (yf) has never occurred in asia remains an "unsolved mystery" in global health. most countries in asia with high aedes aegypti mosquito density are considered "receptive" for yf transmission. recently, health officials in sri lanka issued a public health alert on the potential spread of yf from a migrant group from west africa. we performed an extensive review of literature pertaining to the risk of yf in sri lanka/south asian region to understand the probability of ac ...	2013	24367789
fever versus fever: the role of host and vector susceptibility and interspecific competition in shaping the current and future distributions of the sylvatic cycles of dengue virus and yellow fever virus.	two different species of flaviviruses, dengue virus (denv) and yellow fever virus (yfv), that originated in sylvatic cycles maintained in non-human primates and forest-dwelling mosquitoes have emerged repeatedly into sustained human-to-human transmission by aedes aegypti mosquitoes. sylvatic cycles of both viruses remain active, and where the two viruses overlap in west africa they utilize similar suites of monkeys and aedes mosquitoes. these extensive similarities render the differences in the ...	2013	23523817
blood meal induced microrna regulates development and immune associated genes in the dengue mosquito vector, aedes aegypti.	aedes aegypti is a blood-feeding mosquito that transmits human pathogens such as dengue virus, yellow fever virus and chikungunya virus. recently, dramatic changes in the transcriptome of ae. aegypti following a blood meal have been reported; however, the molecular factors involved in regulating these changes are largely unknown. in this study, we found induction of a number of endogenous micrornas (mirnas) in blood fed (bf) mosquitoes. one of these mirnas, aae-mir-375, was only detected in bf m ...	2013	23202267
bicluster pattern of codon context usages between flavivirus and vector mosquito aedes aegypti: relevance to infection and transcriptional response of mosquito genes.	the mosquito aedes aegypti is the primary vector of dengue virus (denv) infection in most of the subtropical and tropical countries. besides denv, yellow fever virus (yfv) is also transmitted by a. aegypti. susceptibility of a. aegypti to west nile virus (wnv) has also been confirmed. although studies have indicated correlation of codon bias between flaviviridae and their animal/insect hosts, it is not clear if codon sequences have any relation to susceptibility of a. aegypti to denv, yfv and wn ...	2014	24838953
spatio-temporal distribution of aedes aegypti (diptera: culicidae) mitochondrial lineages in cities with distinct dengue incidence rates suggests complex population dynamics of the dengue vector in colombia.	aedes aegypti is the primary vector of the four serotypes of dengue virus (denv1-4), chikungunya and yellow fever virus to humans. previous population genetic studies have revealed a particular genetic structure among the vector populations in the americas that suggests differences in the ability to transmit denv. in colombia, despite its high epidemiologic importance, the genetic population structure and the phylogeographic depiction of ae. aegypti, as well as its relationship with the epidemio ...	2015	25893246
natural transovarial transmission of dengue virus 4 in aedes aegypti from cuiabá, state of mato grosso, brazil.	dengue is the most prevalent arboviral disease in tropical areas. in mato grosso, outbreaks are reported every year, but studies on dengue in this state are scarce.	2015	25860459
effects of proteinase inhibitor from adenanthera pavonina seeds on short- and long term larval development of aedes aegypti.	currently, one of the major global public health concerns is related to the transmission of dengue/yellow fever virus by the vector aedes aegypti. the most abundant digestive enzymes in ae. aegypti midgut larvae are trypsin and chymotrypsin. since protease inhibitors have the capacity to bind to and inhibit the action of insect digestive proteinases, we investigated the short- and long-term effects of adenanthera pavonina seed proteinase inhibitor (apti) on ae. aegypti larvae, as well as a possi ...	2015	25796215
[management of the yellow fever epidemic in 2010 in séguéla (côte d'ivoire): value of multidisciplinary investigation].	in august 2010, five positive cases of yellow fever were reported in the region of séguéla, in the northwest of côte d'ivoire, affected by an armed conflict since 2002. in order to evaluate the extent of yellow fever virus circulation and the risk for local people, a multidisciplinary investigation was carried out by the côte-d'ivoire ministry of health and public hygiene.	2015	25629680
zika virus in brazil and the danger of infestation by aedes (stegomyia) mosquitoes.	zika virus, already widely distributed in africa and asia, was recently reported in two northeastern brazilian: state of bahia and state of rio grande do norte, and one southeastern: state of são paulo. this finding adds a potentially noxious virus to a list of several other viruses that are widely transmitted by aedes (stegomyia) aegypti and aedes (stegomyia) albopictus in brazil. the pathology and epidemiology, including the distribution and vectors associated with zika virus, are reviewed. th ...	2016	26689277
zika virus: a new arboviral public health problem.	zika virus (zikv) is a single-stranded rna virus in the flaviviridae family and transmitted to human through infected mosquitos (aedes aegypti and aedes albopictus). virus is closely related with other flaviviruses; dengue virus, yellow fever virus, west nile virus, and japanese encephalitis virus phylogenetically. due to the possible relationship between virus and clinical features including microcephaly, ventricule, and eye deformities, guillain-barre syndrome increases the interest on this vi ...	2016	27469112
infection of mosquito cells (c6/36) by dengue-2 virus interferes with subsequent infection by yellow fever virus.	dengue is one of the most important diseases caused by arboviruses in the world. yellow fever is another arthropod-borne disease of great importance to public health that is endemic to tropical regions of africa and the americas. both yellow fever and dengue viruses are flaviviruses transmitted by aedes aegypti mosquitoes, and then, it is reasonable to consider that in a given moment, mosquito cells could be coinfected by both viruses. therefore, we decided to evaluate if sequential infections o ...	2016	26808727
yellow fever virus capsid protein is a potent suppressor of rna silencing that binds double-stranded rna.	mosquito-borne flaviviruses, including yellow fever virus (yfv), zika virus (zikv), and west nile virus (wnv), profoundly affect human health. the successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. similar to other invertebrate animals and plants, the mosquito's rna silencing pathway comprises its primary antiviral defense. although a diverse range of plant and insect viruses has ...	2016	27849599
rplp1 and rplp2 are essential flavivirus host factors that promote early viral protein accumulation.	the flavivirus genus contains several arthropod-borne viruses that pose global health threats, including dengue viruses (denv), yellow fever virus (yfv), and zika virus (zikv). in order to understand how these viruses replicate in human cells, we previously conducted genome-scale rna interference screens to identify candidate host factors. in these screens, we identified ribosomal proteins rplp1 and rplp2 (rplp1/2) to be among the most crucial putative host factors required for denv and yfv infe ...	2017	27974556
diversity of yellow fever mosquito vectors in the atlantic forest of rio de janeiro, brazil.	environmental modifications caused by human activities have led to changes in mosquito vector populations, and sylvatic species have adapted to breeding in urban areas.	2017	27384833
cell fusing agent virus and dengue virus mutually interact in aedes aegypti cell lines.	the genus flavivirus contains more than 70 single-stranded, positive-sense arthropod-borne rna viruses. some flaviviruses are particularly medically important to humans and other vertebrates including dengue virus (denv), west nile virus, and yellow fever virus. these viruses are transmitted to vertebrates by mosquitoes and other arthropod species. mosquitoes are also infected by insect-specific flaviviruses (isfs) that do not appear to be infective to vertebrates. cell fusing agent virus (cfav) ...	2017	28761113
high-resolution structure of a kazal-type serine protease inhibitor from the dengue vector aedes aegypti.	blood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito aedes aegypti, which is a vector of dengue virus, yellow fever virus, chikungunya virus and zika virus, is no exception. aati is a single-domain, noncanonical kazal-type serine proteinase inhibitor from a. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. here, the 1.4 å res ...	2017	28777090
[arboviruses also have an american dream].	some arboviruses that originated in the old world have been introduced by humans into the american continent. the first of them was the yellow fever virus, coming from the west african coast with slaves in the 17th-19th centuries, followed by dengue viruses, which were always prevalent within the americas. next was thewest nile virus, introduced in new york in 1999, that spread in only a few years over the whole continent. then, chikungunya virus arrived on saint martin island in 2013 after its ...	2017	28509947
assessment of risk of dengue and yellow fever virus transmission in three major kenyan cities based on stegomyia indices.	dengue (den) and yellow fever (yf) are re-emerging in east africa, with contributing drivers to this trend being unplanned urbanization and increasingly adaptable anthropophilic aedes (stegomyia) vectors. entomological risk assessment of these diseases remains scarce for much of east africa and kenya even in the dengue fever-prone urban coastal areas. focusing on major cities of kenya, we compared den and yf risk in kilifi county (den-outbreak-prone), and kisumu and nairobi counties (no document ...	2017	28817563
interaction of flavivirus with their mosquito vectors and their impact on the human health in the americas.	some of the major arboviruses with public health importance, such as dengue, yellow fever, zika and west nile virus are mosquito-borne or mosquito-transmitted flavivirus. their principal vectors are from the family culicidae, aedes aegypti and aedes albopictus being responsible of the urban cycles of dengue, zika and yellow fever virus. these vectors are highly competent for transmission of many arboviruses. the genetic variability of the vectors, the environment and the viral diversity modulate ...	2017	28499872
potential risk of re-emergence of urban transmission of yellow fever virus in brazil facilitated by competent aedes populations.	yellow fever virus (yfv) causing a deadly viral disease is transmitted by the bite of infected mosquitoes. in brazil, yfv is restricted to a forest cycle maintained between non-human primates and forest-canopy mosquitoes, where humans can be tangentially infected. since late 2016, a growing number of human cases have been reported in southeastern brazil at the gates of the most populated areas of south america, the atlantic coast, with rio de janeiro state hosting nearly 16 million people. we sh ...	2017	28687779