| in vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium. | an efficient and reliable method is described for the culture of equine strongyles from the third (l3) to the fourth (l4) larval stage. medium consists of 50% fetal calf serum and 50% nctc with additions of l-glutamine, nahco3, yeast extract, bactopeptone, and dextrose. the gas phase used is of prime importance; it is a mixture of 10% co2, 5% o2, and 85% n2. strongylus vulgaris, strongylus edentatus, strongylus equinus, triodontophorus brevicauda, triodontophorus serratus, triodontophorus tenuic ... | 1994 | 8158465 |