| non-specific alkaline phosphomonoesterases of eight species of digenetic trematodes. | alkaline phosphatases from different trematodes occupying the same habitat have identical ph otima but different levels of enzyme activities. isoparorchis hypselobagri, from the fish wallago attu, shows four to six times more enzyme activity than fasciolopsis buski, gastrodiscoides hominis and echinostoma malayanum, from the pig sus scrofa, and fasciola gigantica, gigantocotyle explanatum, cotylophoron cotylophorum and gastrothylax crumenifer, from the buffalo bubalus bubalis. at least two peaks ... | 1975 | 1442 |
| phosphatase systems in paramphistomum explanatum fischoeder, 1901. | extracts of adult paramphistomum explanatum have been shown to contain high concentration of acid phosphomonoesterase with maximum activity at ph 4.5. the enzyme has been characterized by an exhibition of an unexpected increase in the inhibitory action of a mercury at 1 mm concentration by edta. with a lower concentration of mercury (0.1 mm and below) edta gave partial protection against inhibition. different concentrations of magnesium and cobalt activated the enzyme while fluoride, copper, ars ... | 1975 | 238347 |
| comparative pathological study of hepatic changes induced by fasciola gigantica and gigantocotyle explanatum in javanese thin-tailed sheep. | pathological changes in the liver induced by fasciola gigantica and gigantocotyle explanatum were readily distinguishable from each other. lesions associated with the migration of immature flukes through the parenchyma were a prominent feature of infection with fasciola gigantica, whereas lesions induced by gigantocotyle explanatum were confined to the large bile ducts. the size of the hepatic lesions increased during the course of infection with fasciola gigantica and was associated with the fo ... | 1991 | 1779036 |
| seasonal reproduction in paramphistomum epiclitum and gastrothylax crumenifer, rumen paramphistomes of the indian water buffalo, and comparison with the biliary paramphistome gigantocotyle explanatum. | | 1988 | 3417379 |
| sensory receptors of the miracidium of gigantocotyle explanatum (trematoda:paramphistomidae). | | 1987 | 3654053 |
| ultrastructural and histochemical observations on the foregut and gut caeca of gigantocotyle explanatum, gastrothylax crumenifer and srivastavaia indica (trematoda:paramphistomidae). | | 1987 | 3654054 |
| study of phosphagens in two trematodes. | extracts from the adults of two trematodes fasciola gigantica and paramphistomum explanatum have been shown to contain three phosphagens, phosphoarginine, phosphocreatine, and phosphoglycocyamine. the three phosphokinases involved in the transphosphorylation of these phosphagens in both worms were inhibited by edta and mercuric ions while iodoacetate had no effect. | 1980 | 6245542 |
| the occurrence of paramphistomes in ruminants in iraq with a description of gigantocotyle explanatum in cattle and bufaloes. | | 1970 | 5525827 |
| histochemical observations on the spermatogenesis of the trematode, paramphistomum (explanatum) bathycotyle. | | 1970 | 5487417 |
| pathological lesions in livers of two indian sambars (cervus unicolor niger) infected with paramphistomum explanatum creplin, 1847; nasmark, 1937 gigantocotyle explanatum. | | 1969 | 5389383 |
| studies on the ultrastructure and histochemistry of the lymph system in three species of amphistome (trematoda: digenea) gigantocotyle explanatum, gastrothylax crumenifer and srivastavaia indica from the indian water buffalo bubalus bubalis. | the lymph system of three amphistome parasites from buffaloes, gigantocotyle explanatum, gastrothylax crumenifer and srivastavaia indica was studied using light microscope histochemistry and electron microscopy. in each case the system comprised a single pair of main longitudinal vessels which gave rise to numerous sub-dividing lateral branches. although the finer lymph channels associated with most internal systems, they did not penetrate the basement membrane of any organ. the lymph vessels we ... | 1985 | 3989258 |
| ultrastructural and histochemical observations on the epidermis, presumptive tegument and glands of the miracidium of gigantocotyle explanatum (trematoda:paramphistomidae). | | 1987 | 3583536 |
| gigantocotyle explanatum: scanning electron microscopic studies on the topographical effects of certain anthelmintics in vitro. | | 1987 | 3429120 |
| biochemical variations in gigantocotyle explanatum and gastrothylax crumenifer with respect to their seasonal reproduction. | biochemical components, glycogen, protein, nucleic acids and lipid fractions were analysed every month from january to december 1986, in the liver and rumen amphistomes gigantocotyle explanatum and gastrothylax crumenifer, respectively. the results reveal a considerable seasonal variation. in the rumen amphistome the components reach their maximum level only once a year, whereas in the liver amphistome, more than one peak is observed in a year. in g. crumenifer the content of glycogen and nuclei ... | 1990 | 2312219 |
| histochemical characterization of esterases from three species of amphistomes. | esterase activity of gigantocotyle explanatum, ceylonocotyle scoliocoelium and cotylophoron cotylophorum were localized histochemically in various regions associated with nutrition, viz, integument, pharynx, sucker and gastrodermis. the intensity of the reactions varied in the different regions of the same parasite and also from species to species. the distribution and relative activity of the esterases were investigated and the results reveal that the influence of inhibitors/activators on enzym ... | 1990 | 2257757 |
| lactate dehydrogenase in two digenetic trematodes and their host. | polyacrylamide gel electrophoresis of the two digenetic trematodes, gigantocotyle explanatum from the liver and gastrothylax crumenifer from the rumen of the water buffalo, bubalus bubalis revealed the presence of at least six and seven isoenzymes of lactate dehydrogenase (ldh), respectively in a partially purified enzyme preparation. the respective host tissues showed five isoenzymes of ldh, which are characteristic to the vertebrates. both parachloromercuribenzoate and iodoacetate affected the ... | 1990 | 2074142 |
| characterization and localization of saposin-like protein-2 (sap-2) in fasciola gigantica. | fasciola gigantica saposin-like protein-2 (fgsap-2) belongs to a family of lipid-interacting proteins that are involved in the cytolysis of target cells. in this study, we have cloned and expressed fgsap-2 and produced the antibody against this recombinant protein. rabbit antiserum against rfgsap-2 reacted with a similar native protein in the whole body extracts of the 4-week-old juvenile and adult stage, as well as a protein in their excretion-secretion, but not in the tegument. in situ hybridi ... | 2010 | 21188603 |
| biochemical changes during the development of the miracidium of gigantocotyle explanatum. | analysis of various biochemical components during the development of the miracidium of g. explanatum showed marked changes, particularly in glycogen, protein and dna levels. though the total lipids remained more or less unchanged, alterations in the levels of cholesterol, triglycerides, free fatty acids, phospholipids and phospholipid fractions were also recorded. such changes could be intrinsically programmed for the cellular differentiation and organogenesis in larval amphistomes. | 1991 | 1757203 |
| spectrophotometric analysis of haemoglobins of some digenetic trematodes and their hosts. | the haemoglobins of six different species of trematodes: gastrothylax crumenifer, srivastavaia indica, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri and their three different hosts: bubalus bubalis, sus scrofa, and wallago attu were spectrophotometrically investigated, and were found to contain porphyrin ix as the common prosthetic group. oxyhaemoglobin, carbonmonoxy-haemoglobin and reduced haemoglobin of all 6 species of trematodes and their 3 ... | 1976 | 1010924 |
| trematodes of domestic ruminants of afghanistan and their role in pathology. | seven trematode species were found in the bile ducts and rumen of domestic ruminants: fasciola gigantica, f. hepatica, gigantocotyle explanatum, g. siemense, paramphistomum epiclitum, fischoederius cobboldi and gastrothylax cruminifer. the parasites caused marked pathological changes in the liver and rumen. | 1976 | 1010490 |
| alkali denaturation of oxyhaemoglobins of some digenetic trematodes and their hosts. | oxyhaemoglobins of six digenetic trematodes,--srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum, fasciolopsis buski, gastrodiscoides hominis, isoparorchis hypselobagri, and of their 3 vertebrate hosts, bubalus bubalis, sus scrofa, wallago attu, were subjected to alkali denaturation at a ph of 12.4. all oxyhaemoglobins from trematodes and their hosts differ from each other in the rate and extent of alkali denaturation which may be explained due to variations in the amino acid ... | 1977 | 599278 |
| effect of some--sh and other reagents on aspartate aminotransferase and l-alanine aminotransferase of paramphistomum explanatum fischoeder, 1901. | studies on aspartate aminotransferase (got) and l-alanine aminotransferase (gpt) of paramphistomum explanatum have shown that gpt activity has more than twice the activity of got. the effect os some--sh reagents like cadmium, mercury, silver and iodoacetamide revealed that both enzymes were inhibited except that got was insensitive to cadmium ions. gpt was found to be much more sensitive to--sh reagents than got. there was unusual reaction to the two thiols used, cysteine and mercaptoethanol. cy ... | 1978 | 418589 |
| [the karyotype of paramphistomum explanatum (creplin, 1849) obtained from korean cattle] | as a series of systematic classification of paramphistomes, in the first step, paramphistomes in the rumen and reticulum were collected on 170 korean cattles (2-3 years age, male) slaughtered at jeonju abattoir from july 1984 to september 1985 and were classified by means of morphology of the worms. afterwards, the karyotype of paramphistomum explanatum (creplin, 1849) which is the common in korean cattle was detected by means of modified air-drying method from testis cells of the worm. the foll ... | 1986 | 12886106 |
| monoamine oxidase in amphistomes and its role in worm motility. | the quantitative assay of mitochondrial monoamine oxidase (mao) activity revealed a higher enzyme level in explanatum explanatum than gastrothylax crumenifer. the specific mao inhibitors, chlorgyline, pargyline, deprenyl and nialamide produced different degrees of interspecific inhibition. the differential effects on enzyme activity of chlorgyline and deprenyl suggests the possible existence of polymorphic forms of the enzyme, mao-a and mao-b, in amphistomes. these specific inhibitors also had a ... | 2000 | 11138015 |
| functional differentiation in trematode hemoglobin isoforms. | the hbs and the major electrophoretic hb components (isohbs) were isolated from three species of the trematodes, explanatum explanatum (ee), gastrothylax crumenifer (gc) and paramphistomum epiclitum (pe), that parasitise the common indian water buffalo bubalus bubalis. the hbs are monomeric and resemble the so-called nonfunctional mutant hemoglobins that have tyr at b10 or e7 positions (replacing leu and the his residues, respectively). however, they are capable of binding with o2 and co. o2 equ ... | 1999 | 10103000 |
| trematode hemoglobins show exceptionally high oxygen affinity. | ligand binding studies were made with hemoglobin (hb) isolated from trematode species gastrothylax crumenifer (gc), paramphistomum epiclitum (pe), explanatum explanatum (ee), parasitic worms of water buffalo bubalus bubalis, and isoparorchis hypselobagri (ih) parasitic in the catfish wallago attu. the kinetics of oxygen and carbon monoxide binding show very fast association rates. whereas oxygen can be displaced on a millisecond time scale from human hb at 25 degrees c, the dissociation of oxyge ... | 1998 | 9675199 |
| comparative spectral, electrophoretic and isoelectric properties of trematode haemoglobins. | a comparative study of the spectral, electrophoretic and isoelectric properties of the haemoglobins of three trematodes, paramphistomum epiclitum, gigantocotyle explanatum and gastrothylax crumenifer was carried out. a high absorption in the beta band region indicates that trematode haemoglobins have high oxygen affinities. electrophoretic mobilities of all trematode and their host haemoglobins were different. the isoelectric points of trematode haemoglobins were found to focus in the acidic ran ... | 1993 | 8288851 |
| [3h]-amino acid uptake and metabolic studies on gigantocotyle explanatum and gastrothylax crumenifer (digenea: paramphistomidae). | the amphistomes gigantocotyle explanatum and gastrothylax crumenifer utilize leucine, alanine, proline and methionine during in vitro incubations. autoradiography on sections of these flukes reveal a time-dependent differential incorporation of tritium-labelled amino acids in various tissues. the tegument appears to be the primary surface through which amino acids are absorbed. following absorption, the reappearance of [3h]-leucine and [3h]-alanine on the tegumental surface during late chase per ... | 1995 | 7635632 |
| effects of some sugars, amino acids, glycerol and alpha-glycerophosphate on oxygen uptake of some digenetic trematodes. | effect of various substrates on the oxygen uptake of some digenetic trematodes -- srivastavaia indica, gastrothylax crumenifer, gigantocotyle explanatum from the buffalo, bubalus bubalis and isoparorchis hypselobagri from the fish wallago attu was studied. in all the four species, the effect of various substrates except maltose was found to be stimulatory. the inhibitory or stimulatory effect of these substrates on oxygen uptake reflects the importance of these compounds in the energy metabolism ... | 1980 | 7450612 |
| the effect of five fasciolicides on malate dehydrogenase activity and mortality of fasciola gigantica, fasciolopsis buski and paramphistomum explanatum. | the effect of oxyclozanide, hexachlorophene, nitroxynil, rafoxanide and diamphenethide on malate dehydrogenase activity of homogenates of fasciola gigantica, fasciolopsis buski and paramphistomum explanatum was investigated. the ratio of oxaloacetate reduction to malate oxidation in homogenates of fasciola gigantica, fasciolopsis buski and p. explanatum was 4.5:1, 3.6:1 and 5.2:1 respectively. oxyclozanide and rafoxanide at 10(-3) m inhibited enzyme activity by 100% in homogenates from all three ... | 1981 | 7264272 |
| histochemical and electrophoretic studies on phosphatases of some indian trematodes. | the isoenzymes of acid and alkaline phosphatases and their histochemical localization were studied by polyacrylamide disc gel electrophoresis in four species of trematodes: gigantocotyle explanatum from the liver and gastrothylax crumenifer from the rumen of water buffalo (bubalus bubalis) and echinostoma malayanum and fasciolopsis buski from the small intestine of the pig (sus scrofa). both acid and alkaline phosphatases were present in the tegument, gastrodermis, suckers, testes, ovary, eggs, ... | 1982 | 7096966 |
| gigantocotyle explanatum creplin (trematoda: paramphistomidae) infection of the liver of the buffalo in ceylon. | | 1956 | 13319508 |
| a redescription and life-history of gigantocotyle explanatum (creplin, 1847) nasmark, 1937 (trematoda: paramphistomidae) from india. | | 1958 | 13539715 |
| partial purification and characterization of gigantocotyle explanatum somatic antigens. | soluble extracts of gigantocotyle explanatum, isolated from the liver of buffalo bubalus bubalis were fractionated on sephadex g-200 columns. nine major fractions referred to as f1, f2, f3, f4, f5, f6, f7, f8 and f9 were separated. each fraction was tested by elisa for antigenicity using sera from g. explanatum-infected field buffaloes. fractions f1 and f2 were highly antigenic, f3, f4, f6 and f7 were moderately antigenic and f5, f8 and f9 were poorly antigenic. analyses by sds-page revealed tha ... | 2004 | 15153279 |
| cathepsin l cysteine proteinase in the diagnosis of bovine fasciola gigantica infection. | cathepsin l cysteine proteinase from fasciola gigantica was evaluated for its potential in the early prepatent detection of this helminth infection in bovine calves. five cross-bred bovine calves were experimentally infected with 400 metacercariae/calf and evaluated for anti-cathepsin l antibody response. f. gigantica infection in these calves could be detected 4 weeks post-infection using an elisa, dipstick elisa and western blotting with 100% sensitivity. the antigen was also used to detect f. ... | 2006 | 16300897 |
| immuno-diagnosis of bubaline fasciolosis with fasciola gigantica cathepsin-l and recombinant cathepsin l 1-d proteases. | fasciola gigantica cathepsin-l cysteine proteinase and recombinant cathepsin l 1-d were assessed for their potential in the immuno-diagnosis of f. gigantica infection in buffaloes. a diagnostic elisa, based on these two antigens, was developed to detect antibodies against f. gigantica in water buffaloes. sensitivity of the elisa was assessed using sera from buffaloes experimentally or naturally infected with f. gigantica from f. gigantica endemic areas and its specificity by probing the sera of ... | 2006 | 16647680 |
| prepatent detection of fasciola gigantica infection in bovine calves using metacercarial antigen. | metacercarial antigen of fasciola gigantica was evaluated for early immunodiagnosis of experimental bovine fasciolosis using elisa and western blot. in elisa, the experimental f. gigantica infection was detected as early as 2 weeks post-infection (wpi). the gradual increasing trend of antibody level was observed from 2 to 7 wpi, followed by a plateau, which was maintained up to 14 wpi. in western blot, sera from experimentally infected calves recognized one distinct polypeptide of 21 kda in frac ... | 2006 | 16999031 |
| early detection of fasciola gigantica infection in buffaloes by enzyme-linked immunosorbent assay and dot enzyme-linked immunosorbent assay. | in an attempt to develop a suitable serological test for early detection of fasciola gigantica infection in buffaloes, a group of proteins were isolated from the somatic antigen of the parasite by immunoaffinity chromatography. the process of isolation of the proteins has been standardized and significant level of repeatability was achieved. to test the diagnostic potentiality of the antigens, two serological tests, viz., enzyme-linked immunosorbent assay (elisa) and dot enzyme-linked immunosorb ... | 2008 | 18344058 |
| screening for indian isolates of egg-parasitic fungi for use in biological control of fascioliasis and amphistomiasis in ruminant livestock. | wild isolates of the egg-parasitic fungi paecilomyces lilacinus and verticillium chlamydosporium, obtained from the organic environment of durg, chhattisgarh, india, were subjected to screening for in vitro growth using different media types, range of incubation temperature and ph, and their predatory activity to the eggs of fasciola gigantica and gigantocotyle explanatum. maximum growth of p. lilacinus was obtained in corn-meal agar compared to any other media types. the preferred medium for gr ... | 2008 | 18462555 |
| detection of fasciola gigantica infection in buffaloes by enzyme-linked immunosorbent assay. | the process of isolation of the 27-kda glycoprotein from the somatic antigen of fasciola gigantica was standardized and the diagnostic potentiality was evaluated for the detection of bubaline fasciolosis by indirect enzyme-linked immunosorbent assay. initially, the test was standardized using the sera from experimentally noninfected(n = 20) and infected (n = 5)animals. further, the sensitivity and the specificity of the test were evaluated through the sera of buffaloes with different natural inf ... | 2008 | 18781327 |
| paralytic effect of alcoholic extract of allium sativum and piper longum on liver amphistome, gigantocotyle explanatum. | to investigate the effects of alcoholic extract of allium sativum and piper longum on the muscular activity of a parasitic amphistome, gigantocotyle explanatum. | 2008 | 21279168 |
| development of cathepsin-l cysteine proteinase based dot-enzyme-linked immunosorbent assay for the diagnosis of fasciola gigantica infection in buffaloes. | native cathepsin-l cysteine proteinase (28 kda) was purified from the excretory secretory products of fasciola gigantica and was used for sero-diagnosis of f. gigantica infection in buffaloes by dot-enzyme-linked immunosorbent assay (dot-elisa). the test detected f. gigantica field infection in these animals with a sensitivity of ∼ 90%. no specific igg antibody binding was displayed by sera obtained from 76 buffaloes considered to be fasciola and other parasite-free by microscopic examination of ... | 2012 | 22055612 |
| molecular characterization of veterinary important trematode and cestode species in the mithun bos frontalis from north-east india. | helminth infections in the mithun bos frontalis, including the liver fluke fasciola gigantica, hepato-gastric amphistomes explanatum explanatum, paramphistomum epiclitum and calicophoron calicophorum, and the cestodes echinococcus granulosus and e. ortleppi were studied in north-east india over a 2-year period from 2012 to 2014. cystic echinococcosis caused by e. granulosus and e. ortleppi was found to be highly prevalent in the mithun, with e. ortleppi being reported for the first time. molecul ... | 2016 | 26343353 |
| prevalence and intensity of paramphistomum spp. in cattle from south-eastern iran. | parasitological investigations on paramphistomosis were carried out over a 12-month period in the southeast of iran to determine the prevalence and intensity of this disease. | 2017 | 26246825 |
| protection against fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin l. | fasciola gigantica cathepsin l1h (fgcatl1h) is one of the major cathepsin l released by juveniles of f. gigantica to aid in the invasion of host's tissues. due to its high sequence similarity with other cathepsin l (catl) isoforms of late stage f. gigantica, it was considered to be a good vaccine candidate that can block all catl-mediated protease activities and affect juveniles as well as adult parasites. in this study, recombinant profgcatl1h protein expressed in yeast, pichia pastoris, system ... | 2015 | 25701317 |
| monoclonal antibody against recombinant fasciola gigantica cathepsin l1h could detect juvenile and adult cathepsin ls of fasciola gigantica. | cathepsin ls (catls), the major cysteine protease secreted by fasciola spp., are important for parasite digestion and tissue invasion. fasciola gigantica cathepsin l1h (fgcatl1h) is the isotype expressed in the early stages for migration and invasion. in the present study, a monoclonal antibody (moab) against recombinant f. gigantica cathepsin l1h (rfgcatl1h) was produced by hybridoma technique using spleen cells from balb/c mice immunized with recombinant profgcatl1h (rprofgcatl1h). this moab i ... | 2015 | 25324133 |
| production and characterization of a monoclonal antibody against recombinant saposin-like protein 2 of fasciola gigantica. | a monoclonal antibody (moab) against recombinant fasciola gigantica saposin-like protein 2 (rfgsap-2) was produced by hybridoma technique using spleen cells from balb/c mice immunized with rfgsap-2. this moab is an igg1, κ light chain isotype. by immunoblotting and indirect elisa, the moab reacted specifically with rfgsap-2, the natural fgsap-2 at 10kda in whole body (wb) and excretory-secretory (es) fractions of f. gigantica. it did not cross react with antigens in wb fractions from other paras ... | 2013 | 23164839 |
| 2d-page analysis of the soluble proteins of the tropical liver fluke, fasciola gigantica and biliary amphistome, gigantocotyle explanatum, concurrently infecting bubalus bubalis. | the digenetic trematodes, fasciola gigantica and gigantocotyle explanatum, belonging to the family fasciolidae and paramphistomidae respectively, have been often found to concurrently infect the liver of indian water buffalo bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. in the present study the soluble gene products of both f. gigantica and g. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. the soluble prote ... | 2016 | 27605808 |
| molecular characterization and phylogenetic analysis of explanatum explanatum in india based on nucleotide sequences of ribosomal its2 and the mitochondrial gene nad1. | the aim of this study was to analyze the phylogenetic relationship between explanatum explanatum populations in india and other countries of the indian subcontinent. seventy liver amphistomes collected from four localities in india were identified as e. explanatum based on the nucleotide sequences of ribosomal its2. the flukes were then analyzed phylogenetically based on the nucleotide sequence of the mitochondrial gene nad1 in comparison with flukes from bangladesh and nepal. in the resulting p ... | 2016 | 27523505 |
| molecular and phylogenetic analyses of the liver amphistome explanatum explanatum (creplin, 1847) fukui, 1929 in ruminants from bangladesh and nepal based on nuclear ribosomal its2 and mitochondrial nad1 sequences. | explanatum explanatum flukes, liver amphistomes of ruminants, cause significant economic loss in the livestock industry by inducing severe liver damage. a total of 66 flukes from 26 buffaloes and 7 cattle in four different geographic areas of bangladesh and 20 flukes from 10 buffaloes in the chitwan district of nepal were subjected for analysis. the sequences (442 bp) of the second internal transcribed spacer (its2) of ribosomal dna and the variable fragments (657 bp) of mitochondrial nicotinami ... | 2016 | 27328647 |
| anthelmintic effect of biocompatible zinc oxide nanoparticles (zno nps) on gigantocotyle explanatum, a neglected parasite of indian water buffalo. | helminth parasites of veterinary importance cause huge revenue losses to agrarian economy worldwide. with the emergence of drug resistance against the current formulations, there is a need to focus on the alternative approaches in order to control this menace. in the present study, biocompatible zinc oxide nanoparticles (zno nps) were used to see their in vitro effect on the biliary amphistomes, gigantocotyle explanatum, infecting bubalus bubalis because these nanoparticles are involved in gener ... | 2015 | 26177503 |
| somatic antigens of tropical liver flukes ameliorate collagen-induced arthritis in wistar rats. | parasitic helminths polarize immune response of their vertebrate hosts towards anti-inflammatory th2 type and therefore it is hypothesized that they may suppress the inflammatory conditions in autoimmune disorders. the present study was undertaken to investigate in vivo immunomodulatory and therapeutic potential of somatic antigens (ag) of liver infecting digenetic trematodes [fasciola gigantica (fg) and gigantocotyle explanatum (ge)] in collagen-induced arthritic (cia) wistar rats. the cia rats ... | 2015 | 25992888 |
| morphological and molecular characterization of explanatum explanatum from cattle and buffaloes in myanmar. | a robust molecular marker is needed for discrimination of amphistome species, because identification based on morphology alone requires specialized knowledge and techniques. in this study, we performed morphological and molecular characterization of explanatum explanatum, a species that causes severe liver damage in definitive host species. fifty-five adult amphistomes were collected from cattle and water buffaloes in myanmar. eighteen of the amphistomes, arbitrarily chosen, were morphologically ... | 2013 | 23117826 |
| immunodetection of coproantigens for the diagnosis of amphistomosis in naturally infected indian water buffalo, bubalus bubalis. | the infection of gastrointestinal helminths in livestock is routinely diagnosed by microscopical examination of faecal samples for the presence of ova/eggs but this approach becomes ineffective for the seasonally egg producing trematodes. therefore, an alternative approach to detect the coproantigens of liver and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer respectively, infecting indian water buffalo bubalus bubalis, was undertaken using elisa, immunodot and countercu ... | 2013 | 23036808 |
| characterization of the glutamate dehydrogenase activity of gigantocotyle explanatum and gastrothylax crumenifer (trematoda: digenea). | glutamate dehydrogenase (gldh) (ec 1.4.1.3) is a ubiquitous enzyme, which is present at the protein and carbohydrate metabolism crossroads. the enzyme activity was investigated in biliary and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer, respectively, infecting the indian water buffalo bubalus bubalis. the enzyme activity was consistently higher in g. explanatum as compared to g. crumenifer, where nad(h) was utilized as coenzyme and the ph optima was recorded at 8. the ... | 2009 | 23129890 |
| explanatum explanatum (creplin, 1847) fukui, 1929, in buffaloes in the ahwaz area, southwest iran. | explanatum explanatum (creplin, 1847) fukui 1929, was found in three indigenous buffaloes in the west ahwaz area, southwest iran for the first time. the presence of this amphistome in bile ducts caused remarkable fibrosis of submucosa ad hyperplasia of the endothelial layer, such that villus-like structures and protuberances were formed in large bile ducts of the infected livers. | 1994 | 7886914 |
| incidence and pathological study of explanatum explanatum (creplin, 1847) fukui, 1929 in goats in andhra pradesh, india. | amphistomiasis, caused by species of paramphistomatidae is an economically important disease in ruminants. explanatum explanatum is a very common amphistome in bile ducts and gall bladder of cattle and buffaloes worldwide where as in goats, it is exclusively found in asian countries. screening of livers from 100 sheep and 154 goats during slaughter at local slaughter houses from october, 2014 to april, 2015 in andhra pradesh (india) revealed presence of amphistomes in main bile ducts and gall bl ... | 2017 | 28848273 |
| in vitro effect of metrifonate on the indices of oxidative stress in gigantocotyle explanatum. | helminth infections in general and digenetic trematodes in particular cause a huge economic loss globally to our livestock. gigantocotyle explanatum is a digenetic amphistome that infects the bile ducts of water buffalo and are highly prevalent in tropical and sub-tropical countries. in the present study, effects of an organophosphate compound, metrifonate (mf) in three doses, viz., 9.4 × 10(-5) m (dose i), 14.4 × 10(-5) m (dose ii), and 19.4 × 10(-5) m (dose iii), have been studied in vitro, on ... | 2017 | 28705749 |
| anthelmintic effect of a methanol extract of bombax malabaricum leaves on paramphistomum explanatum. | bombax malabaricum (family bombacaceae) is used as anthelmintic in traditional system of medicine in southern punjab of pakistan. the objective of this study was to evaluate the anthelmintic activity of the methanol extract of b. malabaricum leaves (mebm). live parasites (trematode: paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline. it was incubated in petri dishes at 37 ± 1°c in media containing either no extract (control) or mebm, the test drug at 10, 25, ... | 2011 | 21853226 |
| natural trematode infection in liver of water buffalo (bubalus bubalis): histopathological investigation. | this study reports the infection of liver and bile ducts, carried out from november 2008 to april 2010, on 730 randomly selected water buffaloes-bubalusbubalis, infected with the amphistome trematode parasite explanatum explanatum (creplin, 1847) fukui, 1929. macroscopic examination revealed massive infection of adult fluke in bile ducts and intrahepatic ductules in 131 (18%) cases. the predominant features were multifocal granulomatous nodules throughout the luminal surface of the bile ducts. h ... | 2011 | 22654314 |
| production and characterization of a monoclonal antibody specific to 16 kda antigen of paramphistomum gracile. | a number of monoclonal antibodies (moabs) against the 16 kda antigen of paramphistomum gracile (16 kdaagpg) were produced in vitro by hybridoma technique. reactivity and specificity of these moabs were evaluated by elisa and immunoblotting assays. seven moab clones were selected from the stable hybridoma clones, namely 1d10, 2d7, 3b10, 3d9, 4f1, 4g4, and 5g12. it was found to be igm and kappa light chain isotypes. by immunoblotting and elisa, all moabs reacted with purified 16 kdaagpg at molecul ... | 2017 | 27770198 |
| production and characterization of a monoclonal antibody against recombinant cathepsin l1 of fasciola gigantica. | monoclonal antibodies (moabs) against a recombinant cathepsin l1 of fasciola gigantica (rfgcatl1) were produced in vitro by fusion of balb/c mice spleen cells immunized with rfgcatl1 and mouse myeloma cells. reactivity and specificity of these moabs were evaluated by indirect elisa and immunoblotting techniques. seven moab clones were selected from the stable hybridoma clones, namely 1e10, 1f5, 3d11, 4b10, 4d3, 4e3 and 5e7. clones 1e10, 1f5 and 3d11 were igm, whereas clones 4b10, 4d3, 4e3 and 5e ... | 2014 | 24736227 |
| anthelmintic effect of a methanol extract of leaves of dregea volubilis on paramphistomum explanatum. | dregea volubilis (family asclepediaceae) is widely used as anthelmintic in traditional system of medicine in eastern and southern part of india. the aim of this study was to evaluate the anthelmintic activity of the methanol extract of d. volubilis leaves (medv) and to observe its effect through sem study. live parasites (trematode paramphistomum explanatum) were collected from buffalo in 0.9% phosphate-buffered saline (pbs). it was incubated at 37?±?1°c in media containing either no extract (co ... | 2011 | 21789584 |
| quantitative studies on acetylcholinesterase in seven species of digenetic trematodes. | quantitative estimation of absolute levels and in vitro release of acetylcholinesterase (ache) in seven species of digenetic trematodes: isoparorchis hypselobagri from the swim bladder of catfish, wallago attu; srivastavaia indica and gastrothylax crumenifer from the rumen, and gigantocotyle explanatum from the liver of the water buffalo, bubalus bubalis; fasciolopsis buski, echinostoma malayanum from the small intestine and gastrodiscoides hominis from the caecum of the pig, sus scrofa reveale ... | 1977 | 562036 |
| pathology of caprine liver infested with gigantocotyle explanatum (creplin 1847). | | 1969 | 5815285 |