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bacteroides gingivalis, bacteroides intermedius and actinobacillus actinomycetemcomitans in human periodontal diseases.bacteroides gingivalis, bacteroides intermedius and actinobacillus actinomycetemcomitans seem to be major pathogens in advancing periodontitis in man. first, these organisms are recovered in higher prevalence and proportions from progressive periodontitis lesions than from quiescent periodontal sites. second, antibody levels against b. gingivalis and a. actinomycetemcomitans are markedly elevated in serum and gingival crevice fluid of periodontitis patients compared to normal controls. third, b. ...19883279073
intragingival occurrence of actinobacillus actinomycetemcomitans and bacteroides gingivalis in active destructive periodontal lesions.a total of six active and six nonactive sites from six untreated periodontitis patients were examined for intragingival presence of actinobacillus actinomycetemcomitans and bacteroides gingivalis. the active destructive periodontal disease was determined by the "tolerance method." the method of immunoperoxidase was used in the identification of intragingival microorganisms in active and nonactive periodontal sites. light microscopic sections of gingival tissues consecutive to those with gram sta ...19883290428
favorable prognosis of patients with prosthetic valve endocarditis caused by gram-negative bacilli of the hacek group. 19883291611
immunological assays for putative periodontal pathogens. 19863295679
use and interpretation of microbiological assays in periodontal diseases. 19863295682
analysis of major antigens of haemophilus (actinobacillus) pleuropneumoniae and related organisms.outer membrane protein (omp)-enriched extracts and whole-cell protein preparations of haemophilus (actinobacillus) pleuropneumoniae and related organisms were examined by polyacrylamide gel electrophoresis and immunoblotting. both the omp-enriched and whole-cell protein profiles of actinobacillus suis, a. pleuropneumoniae (nad-independent biovar), a. lignieresii, and pasteurella haemolytica were very similar to those of h. pleuropneumoniae serotypes 1 to 8. antisera prepared against h. pleuropne ...19873298061
juvenile periodontitis in finland. 19873303023
association of an abnormality of neutrophil chemotaxis in human periodontal disease with a cell surface protein.localized juvenile periodontitis (ljp) is characterized by severe, early-onset, molar and incisor bone loss; neutrophil chemotaxis disorders; and a high prevalence of actinobacillus actinomycetemcomitans infection. ljp is further characterized by significant familial aggregation of the disease. recent work in our laboratory has demonstrated the selective depletion of a surface glycoprotein of 110,000 mr (gp110) from ljp neutrophils by using surface labeling with [14c]formaldehyde and autofluorog ...19873305369
tissue localization of actinobacillus actinomycetemcomitans in human periodontitis. i. light, immunofluorescence and electron microscopic studies.invasion of periodontal tissues by different bacterial morphotypes has been reported in human periodontitis; however, limited information is available as to prevalence, localization and the bacterial species involved. the present study determined prevalence and gingival localization of actinobacillus (haemophilus) actinomycetemcomitans in periodontal lesions of juvenile periodontitis patients. thirty-five gingival biopsies were obtained from 12 juvenile periodontitis patients at the time of peri ...19873305856
tissue localization of actinobacillus actinomycetemcomitans in human periodontitis. ii. correlation between immunofluorescence and culture techniques.recent immunohistological studies have suggested that actinobacillus actinomycetemcomitans is present in the gingival tissues in juvenile periodontitis lesions. the present study examined tissue bound a. actinomycetemcomitans by bacterial culture and immunohistological demonstration of antigen in tissue. a total of 14 periodontitis lesions were examined. eleven biopsies were obtained from gingiva adjacent to a. actinomycetemcomitans infected pockets, while the remaining three control biopsies we ...19873305857
clinical immunologic and microbiologic features of active disease sites in juvenile periodontitis.eight juvenile periodontitis (jp) patients with progressing disease were evaluated for clinical, immunologic, and microbiologic features. clinically, bleeding on probing, pocket depth, and attachment level were unrelated to progressing disease. only actinobacillus actinomycetemcomitans was related to a marked increase in attachment loss when examined on both a site and patient basis. eikenella corrodens was significantly elevated in progressing sites with a. actinomycetemcomitans as opposed to n ...19873316298
rapidly progressive periodontitis. neutrophil chemotaxis inhibitory factors associated with the presence of bacteroides gingivalis in crevicular fluid.in the last few years several bacteriological and immunological studies have investigated the role of bacteria and immune defects in order to establish the etiopathogenesis of periodontal disease. with regard to the immune system, a defect in polymorphonuclear neutrophil (pmn) chemotaxis has been frequently reported in patients with rapidly progressive or juvenile periodontitis. the purpose of this study was to investigate in five patients with rapidly progressive periodontitis and normal chemot ...19873323463
replacement therapy of the prevention of dental disease. 19873326613
immunosuppressive properties of actinobacillus actinomycetemcomitans leukotoxin.actinobacillus actinomycetemcomitans produces a leukotoxin that kills human polymorphonuclear cells (pmns) and monocytes but not lymphocytes. in this study, we examined a. actinomycetemcomitans leukotoxin for its ability to alter human peripheral blood lymphocyte (hpbl) responsiveness. after a 90-min exposure to the leukotoxin, all monocytes were killed and hpbl responsiveness to mitogens and antigens was significantly inhibited. the ability of the leukotoxin to inhibit hpbl responses was not su ...19883335399
actinobacillus actinomycetemcomitans and endocarditis. 19883343530
actinobacillosis in free-ranging snowshoe hares (lepus americanus) from alaska.actinobacillus capsulatus was isolated from lung, liver, and/or spleen tissue of three snowshoe hares (lepus americanus) in alaska. this is the first report of the isolation of this bacterium from free-ranging hares. actinobacillus capsulatus may have a negative impact on the population density of hares.19883352090
monoclonal antibodies to actinobacillus actinomycetemcomitans.murine hybridoma cell lines were developed which synthesized monoclonal antibodies against actinobacillus actinomycetemcomitans-associated antigens. monoclonal antibodies specific for an antigen(s) common to all a. actinomycetemcomitans isolates tested but not detected on other gram-negative oral plaque microorganisms or other actinobacillus species were identified. monoclonal antibodies specific for each serotype group of a. actinomycetemcomitans which did not bind to other actinobacillus speci ...19883356470
septic periorchitis in a horse.a 2-month-old standard-bred colt with signs of abdominal pain and large scrotum was found to have septic periorchitis involving the right testis. surgical exploration of the abdomen and scrotum was performed; the colt was then castrated. actinobacillus equuli was isolated from specimens obtained at surgery. the colt was treated with broad-spectrum antibiotics and flunixin meglumine after surgery, and fully recovered. the clinical signs of periorchitis in the colt were similar to an inguinal/scro ...19883356574
atypical actinobacillosis granulomas in cattle.actinobacillus lignieresii is well recognized as the cause of "wooden tongue." we have encountered actinobacillosis as the cause of granulomas in unusual locations. these lesions have been confused with neoplasms, nasal polyps, or exuberant granulation tissue. four such cases constitute this report.19883371003
serological studies of actinobacillus (haemophilus) pleuropneumoniae strains of serotype 6 and their antigenic relationship with other serotypes.serological tests such as agglutination, coagglutination, precipitation and indirect haemagglutination were used to study the antigenic relationship of reference and field strains of actinobacillus (haemophilus) pleuropneumoniae of serotype 6 with reference strains of other serotypes. both cell-associated particulate and cell-free soluble antigens prepared from unheated and heat-treated bacterial suspensions of reference and field strains of serotype 6 were used in the studies. species-specific, ...19883381462
electron microscopic examination of capsular material from various serotypes of actinobacillus pleuropneumoniae.the capsular material on pplo broth-grown cells of actinobacillus pleuropneumoniae representing serotypes 1 to 10 was visualized by transmission electron microscopy after polycationic ferritin labeling and also after stabilization with specific antibodies. all the isolates examined were covered with a layer of capsular material whose thickness varied between 80 to 90 nm and 210 to 230 nm when examined by immunostabilization. we were also able to visualize a. pleuropneumoniae in lungs of infected ...19883384814
isolation of actinobacillus (haemophilus) pleuropneumoniae serovar 1, 6, or 7 from pigs. 19883386118
virulence properties and protective efficacy of the capsular polymer of haemophilus (actinobacillus) pleuropneumoniae serotype 5.the role of the capsule of haemophilus (actinobacillus) pleuropneumoniae serotype 5 in bacterial virulence, and the protective efficacy of antibody to serotype 5 capsule was investigated. encapsulated h. pleuropneumoniae serotype 5 were resistant to killing by complement and antibody to capsule or somatic antigens, whereas a noncapsulated mutant was sensitive to killing by the alternative complement pathway alone. antiserum to whole h. pleuropneumoniae serotype 5 bacteria or monospecific antiser ...19883397178
natural occurrence of black-pigmented bacteroides species in the gingival crevice of the squirrel monkey.the objective of this study was to determine whether the squirrel monkey (saimiri scuireus) is indigenously colonized with black-pigmented bacteroides (bpb) resembling human bacteroides gingivalis and bacteroides intermedius (suspected periodontal pathogens) and to determine the usefulness of the squirrel monkey as an in vivo model for studying colonization by putative pathogens. we assayed the subgingival plaques of 138 monkeys of various ages and in four different colonies for the presence of ...19883410543
killing of actinobacillus actinomycetemcomitans by human lactoferrin.actinobacillus actinomycetemcomitans is a fastidious, facultative gram-negative rod associated with endocarditis, certain forms of periodontal disease, and other focal infections. human neutrophils have demonstrated bactericidal activity against a. actinomycetemcomitans, and much of the oxygen-dependent killing has been attributed to the myeloperoxidase-h2o2-halide system. however, the contribution of other neutrophil components to killing activity is obscure. lactoferrin, an iron-binding glycop ...19883417349
regulation of hemolysin expression in actinobacillus pleuropneumoniae serotype 1 by ca2+.actinobacillus pleuropneumoniae, the causative agent of swine pleuropneumonia, secretes a hemolytic activity which is thought to be a factor involved in the pathogenesis of the disease. the biosynthesis of hemolysin by serotype 1 strain 4074 was strongly dependent on the activity of free ca2+ in the growth medium. at activities of free ca2+ below 50 microm, very low hemolytic activities could be detected in the growth medium and in cell extracts. maximal hemolytic activities of up to 400 hemolyt ...19883417350
evaluation of heat-sensitive, neutrophil-toxic, and hemolytic activity of haemophilus (actinobacillus) pleuropneumoniae.cytotoxic and hemolytic activity of haemophilus (actinobacillus) pleuropneumoniae serotype 1 strain cm5 was investigated because of the potential role as a virulence determinant. viable bacteria were toxic for porcine and bovine neutrophils, whereas bacteria killed by heat treatment at 60 c for 1 hour were not. similarly, bacteria-free culture supernatant was cytotoxic and hemolytic in assays that used porcine neutrophils and erythrocytes, whereas supernatant treated at 60 c for 1 hour had no ac ...19883421527
an in vitro model to study bacterial invasion of periodontal tissues.in periodontal disease, the abilities of bacteria to adhere to and degrade in vivo basement membranes should be considered as two of the rate-limiting steps for the potential active or passive invasion of gingival connective tissues. to study these mechanisms in greater detail, we used the pf hr-9 basement-membrane-like matrix to establish an in vitro model of bacterial invasion and degradation. three gram-negative anaerobic periodontopathic organisms, bacteroides gingivalis, fusobacterium nucle ...19883422291
binding patterns of five monoclonal antibodies to actinobacillus (haemophilus) pleuropneumoniae.five monoclonal antibodies were obtained after immunising mice with superficial antigens of three strains representing serovars 1 to 3 of actinobacillus (haemophilus) pleuro-pneumoniae. when tested in elisa against the standard strains representing serovars 1 to 10, the monoclonal antibody raised against the standard strain of serovar 1 reacted only with that strain. of the three monoclonal antibodies raised against the standard strain of serovar 3, one reacted with serovars 3 and 8 only, anothe ...19873444990
isolation of actinobacillus seminis and histophilus ovis strains from aborted ovine fetuses in hungary. 19873447463
actinobacillus actinomycetemcomitans and clinical periodontal status in finnish juvenile periodontitis patients.the relationship between the clinical periodontal status and the occurrence of actinobacillus actinomycetemcomitans (a.a.) in 19 finnish patients with localized juvenile periodontitis (ljp) was studied. clinical examination included the plaque index, gingival index, suppuration, probing depth and bleeding on probing. the subgingival bacterial samples were taken from two diseases periodontal pockets with radiographic bone loss and two periodontal pockets exhibiting no radiographic alveolar bone l ...19863457136
the habitat of periodontopathic micro-organisms.the purpose of the present study was to investigate the significance of oral mucosal surfaces as a habitat of periodontopathic micro-organisms. for this investigation, 7 young adults were selected for an experimental gingivitis study. at the start of the experiment, bacterial samples were taken by means of a sterile swab from 4 oral mucosal surfaces: the buccal mucosa, the vestibular fold, the dorsum of the tongue and a tonsil. all individuals were asked to abstain from oral hygiene procedures i ...19863457811
dynamics of infection by leukotoxic strains of actinobacillus actinomycetemcomitans in juvenile periodontitis.juvenile periodontitis is associated with a high incidence of infection by actinobacillus actinomycetemcomitans (aa). the data presented indicate that the ability of aa to destroy human pmns is altered during the course of infection. leukotoxic strains of aa are characteristically found in isolates obtained from younger patients (6-12 years of age) but not in older subjects (13-25 years old). this suggests that the leukotoxin may be more important during early as opposed to more advanced phases ...19863458728
localized juvenile periodontitis of the primary dentition.a case report of an 8-year-old caucasian boy with localized juvenile periodontitis affecting the primary dentition is described. a method of culturing for a. actinomycetemcomitans, frequently associated with localized juvenile periodontitis, was used to support the diagnosis.19863458732
clinical measurements of periodontitis.clinical measurement of periodontitis has historically focused on the concept of periodontitis as a slow, continuous process which has emphasized measurements of the static condition of periodontal pockets. observations based on longitudinal measurement of attachment loss in untreated subjects have indicated that periodontal destruction occurs in discrete episodes of short duration. based on these studies, it has been suggested that chronic periodontal disease proceeds through a series of random ...19863459739
[immunobiological properties and chemical composition of cellular and extracellular endotoxic substances of actinobacillus actinomycetemcomitans]. 19863459783
verification of juvenile periodontitis by bacteriological identification. 19863459856
the effect of actinobacillus actinomycetemcomitans (aa) on lymphocyte transformation in patients with juvenile periodontitis. 19863460756
the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius in destructive periodontal disease in adults.a total of 235 subgingival sites, including 104 progressive deep lesions from 61 untreated patients, 26 progressive deep lesions from 10 treated patients, 33 nonprogressive deep sites from 20 untreated patients, and 72 nonprogressive sites from 55 treated patients were examined for actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. the periodontal disease progression was mainly determined on the basis of radiographic changes in the crestal alveolar bone lev ...19863462204
frequency distributions of periodontal attachment loss. clinical and microbiological features.the present investigation attempted to determine if the pattern of past periodontal destruction could be concisely summarized, and related to other clinical and microbiological parameters. 61 subjects between the ages of 12 and 61 years with destructive periodontal disease were evaluated at 6 sites per tooth for redness, plaque, suppuration, bleeding on probing, pocket depth, and attachment level. the frequency distribution of baseline attachment level measurements was computed for each individu ...19863462210
subgingival microflora and periodontal conditions in healthy teenagers.the composition of subgingival flora was correlated with clinical periodontal conditions in 100 teenagers aged 12 to 17 years. the community periodontal index of treatment needs (cpitn) was used for the clinical examination. subgingival bacterial samples were taken from the mesial surface of each first molar, two samples for dark-field microscopy and two samples for actinobacillus actinomycetemcomitans (a.a.) cultivation. fifty-nine subjects had at least three healthy sextants. score 1 was the h ...19863462383
antibodies to actinobacillus actinomycetemcomitans in a korean population.previous studies demonstrated that serum antibodies to actinobacillus actinomycetemcomitans strain y4 are significantly elevated in sera from localized juvenile periodontitis (ljp) and postlocalized juvenile periodontitis (p-ljp) patients compared to normal controls in united states populations. this study examined the age of subjects in relation to the antibody levels to a. actinomycetemcomitans in a korean population. seven groups were investigated including sera from newborns, infants, childr ...19863462384
[current trends in periodontology. juvenile periodontitis]. 19863462491
[differentiation among the related groups of bacteria actinobacillus, haemophilus, pasteurella, bacteroides and streptococcus using instrumental analysis and bio-enzymatic methods]. 19863462665
serum antibody levels to 4 periodontal pathogens remain unaltered after mechanical therapy of juvenile periodontitis.serum antibody titers to actinobacillus actinomycetemcomitans (a.a.) y4, bacteroides gingivalis, capnocytophaga ochracea and eubacterium saburreum were determined with an enzyme immunoassay in 12 patients with juvenile periodontitis (jp) at the beginning of treatment and 16 to 32 months later after clinically successful mechanical therapy without antibiotics. the values were compared to corresponding data obtained from 26 age- and sex-matched individuals with a healthy periodontium. 6 jp patient ...19863463569
occurrence of actinobacillus actinomycetemcomitans and spirochetes in relation to age in localized juvenile periodontitis.occurrence of actinobacillus actinomycetemcomitans (a.a.) and spirochetes was examined in relation to the age of patients suffering from localized juvenile periodontitis (ljp). twenty ljp patients were divided into three age groups: 14-16, 17-19, and 20-25 years of age. two sites with radiographic alveolar bone loss and two sites without bone loss were examined in each patient. probing depth, gingival bleeding after probing, and orthopantomographic alveolar bone loss were recorded. subgingival b ...19863463725
submandibular space abscess due to actinobacillus actinomycetemcomitans.a submandibular space abscess is reported in which a pure culture of actinobacillus actinomycetemcomitans was identified. the bacterium may often be overlooked as a pathogen due to its slow growth and its requirement for carbon dioxide for primary isolation. as a. actinomycetemcomitans is often resistant to commonly used antibiotics, proper management is based on careful utilization of microbiologic tests and clinical judgement. in this case prompt surgical drainage and appropriate antibiotic th ...19863465933
the bacteriology of sheep periodontitis with special reference to actinbacillus actinomycetemcomitans. 19863466409
bacterial specificity in adult periodontitis. a summary of recent work.3 studies investigated the importance of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius in progressive adult periodontitis. a total of 196 adults with advanced periodontitis and 475 periodontal sites were examined. the periodontal disease activity was assessed by changes in probing periodontal attachment level and crestal alveolar bone level. the composition of the subgingival microflora was determined by nonselective and selective culturing and indirect ...19863466908
topical antimicrobial therapy and diagnosis of subgingival bacteria in the management of inflammatory periodontal disease.bacterial specificity in human periodontal diseases suggests the possibility of diagnosing and treating periodontitis as specific infections and using microbiological diagnostic means to evaluate the efficacy of periodontal therapy. in a series of clinical trials, the usefulness of topical antimicrobial agents in combination with surgical and non-surgical conventional periodontal therapy were tested. the healing result was estimated by monitoring probing attachment levels (pal). the usefulness o ...19863466909
[viral infection due to actinobacillus actinomycetemcomitans--the true reason for periodontitis in children and young adults?]. 19863468480
microbiology in periodontics. 19863468648
the capability of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius to indicate progressive periodontitis; a retrospective study.this study evaluated the statistical association of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius with progressive periodontitis. 146 adults with a history of advanced periodontitis contributed 105 "nonprogressing" and 130 "progressing" periodontal sites. periodontal disease activity was assessed by radiographic changes in crestal alveolar bone level. the subgingival proportion of the 3 test bacteria was determined by selective and nonselective culturin ...19873469226
rapidly progressive periodontitis in a patient with hyperthyroidism. 19863469416
bacteriophage infection--a possible mechanism for increased virulence of bacteria associated with rapidly destructive periodontitis.we have recently isolated several groups of bacteriophages infecting actinobacillus actinomycetemcomitans from periodontal lesions in patients with rapidly destructive periodontitis. bacteriophage infection of these bacteria in these patients was restricted to periodontal pockets showing radiographic evidence of recent bone loss and suggests an association between phage-infected a. actinomycetemcomitans and active periodontal disease. on the basis of the biological activity of bacteriophages we ...19873471034
association between bacteriophage-infected actinobacillus actinomycetemcomitans and rapid periodontal destruction.actinobacillus actinomycetemcomitans was isolated from periodontal pockets in a patient suffering from prepubertal periodontitis. electron microscopy revealed 3 different groups of bacteriophages in filtrates of subgingival plaque from all the active periodontal lesions. phage infected a. actinomycetemcomitans in this patient was restricted to periodontal pockets which, according to standardized roentgenograms, had shown bone destruction during the past 12 months. a follow-up study of 7 months r ...19873473090
certain bacterial species and morphotypes in localized juvenile periodontitis and in matched controls.subgingival microflora in localized juvenile periodontitis (ljp) was examined by culture of certain bacterial species and by direct dark-field microscopy in affected and unaffected periodontal pockets of 11 patients and in their age- and sex-matched controls. only actinobacillus actinomycetemcomitans (a.a.) correlated to periodontal destruction and occurred most frequently at the affected ljp sites. a.a., bacteroides intermedius and fusobacterium nucleatum were isolated in proportions greater th ...19873473219
dna probe detection of periodontal pathogens. 19863473415
[the prevalence and antibiotic susceptibility of capnocytophaga and actinobacillus actinomycetemcomitans in the periodontal pockets of periodontitis patients with insulin-independent diabetes mellitus]. 19863474309
salivary igg, a parameter of periodontal disease activity? high responders to actinobacillus actinomycetemcomitans y4 in juvenile and adult periodontitis.the concentration of salivary igg and iga and the levels of salivary igg and iga antibodies to actinobacillus actinomycetemcomitans y4 were measured by elisa in 205 persons including patients with juvenile and adult periodontitis as well as healthy subjects. compared to the concentration observed in subjects with a healthy periodontium, a significantly increased concentration of salivary igg was found in 34% of the patients with moderate adult periodontitis and in 57% of the patients with severe ...19873475296
[bacteroides gingivalis and actinobacillus actinomycetemcomitans in periodontal disease in humans]. 19873475668
cell surface hydrophobicity of actinobacillus actinomycetemcomitans y4.oral bacteria colonize the dento-gingival tissues in a selective manner. hydrophobic reactions have been suggested as one of the major mechanisms of adhesion. hydrophobicity of actinobacillus actinomycetemcomitans y4 (aa) cells was studied in vitro using adherence to the liquid hydrocarbon, octane. adherence of aa cells to octane varied from 60-90%, depending on the medium in which they were grown, age of the culture and the buffer in which the assay was carried out. these data suggest that aa i ...19873476511
effect of periodontal therapy on specific antibody responses to suspected periodontopathogens.the effects of clinically successful periodontal therapy were studied in juvenile periodontitis (jp) and rapidly progressive periodontitis (rp) patients and compared with periodontally healthy subjects (hs). serum samples were obtained in 35 hs prior to the study and in 12 of these subjects 3-4 years later. serum samples were obtained from 50 jp patients initially, 9 subjects immediately following surgical therapy and 29 of these subjects 3-4 years later. rp patients provided 46 initial serum sa ...19873476519
structural determinants of activity of chlorhexidine and alkyl bisbiguanides against the human oral flora.we assayed chlorhexidine and a series of its analogues, in which the chlorophenyl terminal substituents were replaced with alkyl chains, for their in vitro antimicrobial activity against the gram-negative and gram-positive oral bacteria. changes in antimicrobial activity were correlated with changes in agent structure for identification of structural criteria which may be important in the optimization of agent activity. chlorhexidine showed substantial antimicrobial activity against the gram-neg ...19873476581
arylaminopeptidase activities of oral bacteria.protease and peptidase enzymes are thought to play a role in the virulence of many oral organisms, especially those associated with periodontal diseases. in order to evaluate the peptidases of periodontopathogens, we compared the arylaminopeptidase activities of bacteroides gingivalis with those of other oral and non-oral bacteria. sixty-three bacterial strains representing the prominent cultivable organisms in human periodontal pockets were tested, including representatives of the black-pigment ...19863478393
serum igg antibodies to lipopolysaccharides in various forms of periodontal disease in man.serum igg antibody titres to lipopolysaccharides (lps) from two strains of actinobacillus actinomycetemcomitans were significantly elevated in juvenile periodontitis compared with other types of periodontal disease and with controls (p less than 0.05). the highest antibody titres to bacteroides gingivalis lps were in juvenile periodontitis, but this difference was significant only against the control group (p less than 0.01). in adult mild periodontitis there were higher antibody levels to lps f ...19863479058
the presence of phage-infected actinobacillus actinomycetemcomitans in localized juvenile periodontitis patients.electron microscopy revealed 2 different types of bacteriophages isolated from actinobacillus actinomycetemcomitans colonizing exclusively diseased sites in 4 patients with localized juvenile periodontitis (ljp). all sites infected with phage were undergoing periodontal destruction, as judged from consecutive routine radiographs. the phages isolated had a wide host range as assessed from their ability to infect a series of reference strains of a. actinomycetemcomitans. a 5th patient harboured no ...19873480296
serum antibody activity against actinobacillus actinomycetemcomitans in chinese patients with periodontitis. 19873482268
[subgingival bacterial flora in relation to clinical conditions in juvenile periodontitis]. 19873484326
endogenous endophthalmitis caused by actinobacillus actinomycetemcomitans.actinobacillus actinomycetemcomitans is a rare cause of infection in humans. it has been mainly reported in cases of endocarditis and destructive periodontal disease. this report describes the case of a 51-year-old female who had an acute onset of anterior iridocyclitis, followed by the appearance of a white nodule on the lens and subsequent involvement of the vitreous. the course of the disease was long and insidious. actinobacillus actinomycetemcomitans was isolated from 3 of 26 blood cultures ...19863492254
dna probe technology for detection of haemophilus influenzae.a 5 kb haemophilus influenzae dna fragment involved in penicillin-binding proteins expression was used as a probe for specific detection of h. influenzae strains. the 32p-labeled probe specificity was assessed by hybridization to bacterial dots and 75 strains were tested. all h. influenzae (18) and h. aegyptius (1) strains reacted very strongly with the probe. the h. influenzae serotypes tested (a, b, and non-typable strains) did not differ in their hybridization. some hybridization was also fou ...19873502611
[serum antibodies of periodontally related microorganisms--changes of the igg antibodies following periodontal treatment]. 19873506006
antimicrobial susceptibility of microorganisms isolated from equine orthopedic patients.positive cultures were obtained from 60 equine orthopedic cases during a 12 year period (1974-1985). these cases consisted of 34 long or cuboidal bone fractures, 13 arthrotomy/arthroscopy procedures for removal or internal fixation of a fracture, 7 proximal splint bone fractures, and 6 facial or mandibular fractures. excluding the 13 arthrotomies, only 10 (21%) of the 47 were open fractures. multiple organisms were isolated from 36 cases (20 long or cuboidal bone fractures, 7 splint bone fractur ...19873507142
comparative studies on the biology of actinobacillus actinomycetemcomitans leukotoxin in primates. 19873507626
actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius: predictors of attachment loss? 19873507628
[immunobiological characterization of actinobacillus actinomycetemcomitans lipopolysaccharide: inducing activity of interleukin-1 production]. 19873509815
clinical and microbiologic effects of single-dose metronidazole or scaling and root planing in treatment of adult periodontitis.sites affected with adult periodontitis were observed for 3 months to compare their clinical and microbiologic responses to a single 2 g dose of metronidazole, scaling and root planing, or no treatment. 2 sites with probing depths greater than or equal to 5 mm in each of 18 female subjects (6 in each treatment group) were evaluated clinically (plaque and bleeding indices, probing depth, attachment loss) and microbiologically (%s of cocci, motile rods, non-motile rods and spirochetes, and of obli ...19863511104
isolation of members of the haemophilus-pasteurella-actinobacillus group from feral rodents.46 feral rodents, including a common vole (microtus arvalis), house mice (mus musculus), muskrats (ondatra zibetica), house rats (rattus rattus) and brown rats (r. norvegicus) were examined for bacteria of the haemophilus-pasteurella-actinobacillus group. haemophilus spp. (only m. musculus examined) were not obtained. all animal species were found contaminated by p. pneumotropica and/or actinobacillus spp. almost all m. musculus (96%) and most rattus spp. (76%) were contaminated by p. pneumotrop ...19863512906
etiologic significance of bacterial isolates from rams with palpable epididymitis.identified and partly identified bacterial isolates were obtained from 48 rams of various breeds that had unilateral or bilateral epididymitis. most of the animals were approximately 1 year of age; a few were older. brucella ovis, actinobacillus spp, corynebacterium spp, haemophilus spp, acinetobacter spp, escherichia coli, moraxella spp, staphylococcus spp, pasteurella spp, streptococcus spp, and chlamydia psittaci were isolated. a vaccine strain of b ovis, isolated species of bacteria, and mix ...19863521407
attachment level changes in destructive periodontal diseases.the present communication attempts to summarize some of the features of attachment loss which are of interest to the clinician and the statistician analyzing data from clinical trials. these include the measurements employed to detect changes in attachment level, the nature of the destructive disease process and the effects of therapy on the attachment level measurements. although there are several difficulties associated with the attachment level measurement, at the present time it appears to b ...19863522651
analysis of data from clinical studies of localized juvenile periodontitis.data from 27 subjects with localized juvenile periodontitis were used to compute estimates of the intraclass correlation coefficient for initial periodontal pocket depth measurements and total subgingival actinobacillus actinomycetemcomitans counts, and for changes from pre- to post-treatment in periodontal pocket depth, probing attachment level and actinobacillus actinomycetemcomitans counts. the estimates for the clinical parameters were considerably higher than those found in a similar study ...19863522652
indicators of periodontal disease activity: an evaluation.it is becoming increasingly apparent that the traditional clinical criteria are inadequate for: determining active disease sites in periodontitis, monitoring quantitatively the response to therapy or measuring the degree of susceptibility to future breakdown. in an attempt to develop objective measures, a wide variety of studies have been undertaken using saliva, blood, plaque and gingival crevicular fluid (gcf) as the specimen source. examination has included: specific bacteria and their produc ...19863522656
use of monoclonal antibodies to identify outer membrane antigens of actinobacillus species.three monoclonal antibodies (lg17, lg30, and lg33) were used to identify outer membrane antigens of actinobacillus sp (as8c isolate) cultured from the epididymides of an infected ram lamb. specificity of the 3 antibodies to as8c antigens was determined by use of bacterial agglutination, the enzyme-linked immunosorbent assay, and the indirect fluorescent antibody test. results of immunoelectron microscopy confirmed that each antibody was specific for epitopes on as8c outer membrane antigens. eval ...19863526999
chemotaxonomy of selected species of the actinobacillus-haemophilus-pasteurella group by means of gas chromatography, gas chromatography-mass spectrometry and bioenzymatic methods.instrumental analytical and bioenzymatic methods were used to differentiate between species of the actinobacillus-haemophilus-pasteurella group. long-chain fatty acids were analysed directly with gas chromatography (gc) without derivatization. gc of trifluoroacetylated whole-cell methanolysates was a rapid method for differentiation. cellular sugars were more suitable for differentiation than fatty acids. d-glycero-d-mannoheptose, the major localization of which was lipopolysaccharide, distingui ...19863528204
diagnosis and treatment of localized juvenile periodontitis.an actinomycetemcomitans can cause localized juvenile periodontitis and certain types of adult periodontitis. optimal treatment of periodontal disease caused by this microorganism requires systemic antibiotic therapy in addition to mechanical debridement of the infected gingival tissues. laboratory techniques are available to assist the practitioner in identifying this microorganism in dental plaque samples.19863528264
an evaluation of the api zym system as a means of identifying haemophilus somnus and related taxa.the commercially available api zym microbiological identification system was evaluated for the rapid identification of haemophilus somnus. eighty-seven isolates of the organism had api zym profiles which were characteristic. the api zym profiles demonstrate clear differences between h. somnus and other genera but suggest a close association to three related organisms. enzyme activity of h. somnus isolates were similar to organisms identified as histophilus ovis, haemophilus agni and strains uqv ...19863530416
detection and serotyping of actinobacillus actinomycetemcomitans isolates on nitrocellulose paper blots with monoclonal antibodies.a combination of blotting of bacterial colonies on nitrocellulose paper discs and immunoenzymatic detection of bound antigens with specific monoclonal antibodies was used to detect and serotype actinobacillus actinomycetemcomitans directly off the initial agar culture dish. the a. actinomycetemcomitans antigens, representative of specific colonies, were identified immunoenzymatically using monoclonal antibody specific for a species-specific antigen. the serotype of the bacteria in colonies was i ...19863531245
treatment of juvenile periodontitis without antibiotics. a follow-up study.20 patients with juvenile periodontitis (jp) were treated with oral hygiene instruction, scaling and root planing, possibly with flap surgery but with no antibiotics. the patients were monitored after 6 to 12 years. re-examination revealed that no probing depth of 7 mm or more existed any longer, and that sites with probing depth of 4 to 6 mm had decreased from 237 to 46. the bone loss scores had changed from 18% (range 2.0 to 48.1) to 14% (range 0 to 44.4) and the bleeding on probing scores fro ...19863531247
current understanding of the aetiology and progression of periodontal disease.there is overwhelming evidence that bacteria cause periodontitis and that they do so by extending apically along the surfaces of the tooth roots and creating pockets. a very complex mixture of microbial species, mostly although not exclusively gram-negative, anaerobic, and motile, is involved. infection probably occurs in a progressive and sequential manner. the bacteria involved include various species of bacteroides, actinobacillus, eikenella, fusobacterium, capnocytophaga, and eubacterium. lo ...19863533787
periodontal health and disease in young people: screening for priority care.gingivitis is the most common chronic disease affecting young people. almost all teenagers exhibit gingival lesions in the interproximal areas of posterior teeth. gingivitis is caused by supragingival bacterial plaque which contains more than 165 species and subspecies. no single organism or group of bacteria can be directly implicated in the development of gingivitis. periodontitis occurs before 20 years of age, is also first found in the interdental areas of posterior teeth and is preceded by ...19863533788
juvenile periodontitis.juvenile periodontitis occurs in children and young adults and can be classified into: periodontitis which occurs in otherwise healthy individuals, and periodontitis which occurs in juveniles with systemic disease. the periodontitis which occurs in otherwise healthy individuals consists of two major forms: juvenile periodontitis, also called periodontosis or localized juvenile periodontitis (ljp), and generalized juvenile periodontitis which includes early onset adult periodontitis, recurrent ne ...19863533789
antibody response of swine to outer membrane components of haemophilus pleuropneumoniae during infection.sera from pigs infected with haemophilus (actinobacillus) pleuropneumoniae were tested for antibodies to outer membrane proteins (omps) of the organism by immunoblotting. convalescent sera were produced in naturally born, colostrum-fed pigs and in cesarean-derived, colostrum-deprived pigs given h. pleuropneumoniae serotype 5 intranasally twice at 5-week intervals. sera, collected at weekly intervals, were reacted with sarkosyl-insoluble, omp-enriched preparations of h. pleuropneumoniae which had ...19863536748
chronic tenosynovitis caused by actinobacillus actinomycetemcomitans.this is the first report of a patient with a history of painless chronic flexor tenosynovitis caused by actinobacillus actinomycetemcomitans. the slow growth of the organism on culture makes early identification difficult. flexor tenosynovitis along with proper antibiotic treatment resulted in complete resolution in this patient.19873559092
serological characterization of actinobacillus pleuropneumoniae strains and proposal of a new serotype: serotype 12. 19863565204
antigenic specificity of convalescent serum from cattle with haemophilus somnus-induced experimental abortion.the antigens of haemophilus somnus recognized by convalescent bovine serum were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting with a protein a-peroxidase conjugate. the same two 76k and 40k antigens were predominant in whole-bacterium preparations and in outer-membrane-enriched, triton x-100-insoluble fractions. the surface location of these two antigens was confirmed by absorbing antiserum with whole, live bacteria. absorption with h. somnus removed a ...19873570470
in vitro antibacterial properties of el-870, a new semi-synthetic macrolide antibiotic.a new macrolide antibiotic, el-870, 20-deoxo-20-(3,5-dimethylpiperidin-1-yl)desmycosin, has been prepared by chemical modification of desmycosin. in vitro, against selected animal bacterial pathogens, it inhibited growth of pasteurella multocida, pasteurella haemolytica, mycoplasma hyopneumoniae, actinobacillus pleuropneumoniae, streptococcus suis, actinomyces pyogenes and certain other bacteria at levels of 6.25 micrograms/ml or less. in general, the mics for gram-negative enteric bacteria have ...19873570967
sequential study of lesion development in experimental haemophilus pleuropneumonia.recently weaned pigs were infected aerogenically with haemophilus (actinobacillus) pleuropneumoniae, serotype 5. at three, six, 12, and 18 hours and one, two, four and seven days after exposure to haemophili a pair of animals were killed and necropsied. pulmonary oedema with multifocal petechial haemorrhages and a diffuse neutrophilic bronchiolitis and alveolitis were observed at three and six hours after infection. focal areas of coagulative necrosis developed in areas of intense suppuration at ...19873589167
isolation and characterization of actinobacillus seminis strains from ovine semen samples and epididymitis. 19873591111
leukotoxic activity in actinobacillus (haemophilus) actinomycetemcomitans isolated from periodontal disease patients.leukotoxic activity in actinobacillus (haemophilus) actinomycetemcomitans isolated from patients with rapidly progressive periodontitis (rp), gingivitis (g), and juvenile periodontitis (jp), and several oral bacteria, was determined by observation of morphological changes in polymorphonuclear leukocytes (pmns). many a. actinomycetemcomitans isolates yielded both rough-surfaced and umbonate-shaped colonies (a-type), and smooth-surfaced and convex-shaped colonies (b-type), when stock cultures were ...19873613993
subgingival microflora in relation to clinical conditions in juvenile periodontitis. 19863615400
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