| [expression of a bacterial gene, cloned in the yeast, saccharomyces cerevisiae]. | vectors allowing cloning of foreign d.n.a. in the yeast saccharomyces cerevisiae have been recently described. we have introduced in this yeast the lac z gene of the bacteria escherichia coli. an active beta-galactosidase, which is absent in the recipient strain, has been detected in transformed yeast. we thus conclude that the bacterial lac z gene is expressed in yeast. we further showed that the enzyme found in the transformed yeast is identical to the bacterial enzyme with respect to size and ... | 1979 | 118813 |
| [influence of the sterilization technic and culture media components on the growth and dissociation of brucella abortus strain 19 in submerged cultures]. | submerged cultures of brucella abortus strain 19 were studied in shaking flasks. the influence of the sterilization methods and the medium composition on the bacterial yield and cellular dissociation were studied. the selected medium was as follows (amounts in g/l): casein pancreatic hydrolizated 30; yeast extract 10; glucose, 30; sodium phosphate dibasic anhydrous 3,3; sodium monobasic monohydrate 9. cell concentration of 8 . 10(10) viable cell/ml was obtained after 48 hours when the medium com ... | 1978 | 119282 |
| biological effects of magnetic fields: studies with microorganisms. | five bacteria and one yeast were grown in magnetic fields of 50-900 gauss with frequencies of 0-0.3 hz and square, triangular, or sine waveform. growth of these microorganisms could be stimulated or inhibited depending upon the field strength and frequency of the pulsed magnetic field. spore germination and mutation frequency were unaffected by the magnetic fields used in this study. | 1979 | 119572 |
| [paraffin oxidizing system of the yeast candida guilliermondii]. | it has been demonstrated that the enzyme system of candida guilliermondii responsible for hydrocarbon oxidation involves nadph-cytochrome c-reductase (ec 1623) and cytochrome p-450. the system is located in the microsomal fraction. cytochrome p-450 synthesis is induced by hexadecane occurring in the medium. the cellular content of cytochrome p-450 varies in the course of the culture growth. there is a correlation between the cellular content of cytochrome p-450 and the synthesis of primary produ ... | 1979 | 119961 |
| the occurrence of candida albicans in lake ontario bathing beaches. | there are inherent weaknesses associated with currently used bacterial fecal pollution indicator systems. fecal pollution indicator data would be more meaningful if supplemented with information relating to the occurrence of pathogens in recreational water. through surveys of four bathing beaches on lake ontario, it was established that the opportunistically pathogenic yeast candida albicans occurs in near shore waters. the beaches surveyed could be differentiated on the basis of bacterial fecal ... | 1979 | 120220 |
| induction of cytochrome p450 in petroleum-assimilating yeast-ii. comparison of protein synthesizing activity in the cells grown on glucose and n-tetradecane. | | 1979 | 120225 |
| growth factors for mycoplasma pneumoniae in yeast and tissue extracts. | | 1979 | 120357 |
| studies on nadph-cytochrome c reductase i: isolation and several properties of the crystalline enzyme from ale yeast. | | 1979 | 120699 |
| treponema succinifaciens sp. nov., an anaerobic spirochete from the swine intestine. | the morphology, the general physiological characteristics, and the energy-yielding metabolism of an obligately anaerobic spirochete isolated from the colon of a swine were studied. electron microscopy showed that the helical spirochetal cells possessed an outer sheath, a protoplasmic cylinder, and 4 periplasmic fibrils in a 2-4-2 arrangement. the spirochete grew in an atmosphere of n2 in prereduced media containing a carbohydrate, nahco3, rumen fluid, yeast extract, peptone, l-cysteine, and inor ... | 1979 | 120726 |
| antibodies to candida species after operations on the large intestine: observations on the association with oral and faecal yeast colonization. | the occurrence of agglutinating and precipitating antibodies to candida species was determined in 50 patients undergoing operations on the large intestine. the number of patients with oral or faecal yeast colonization and the amount of faecal colonization increased during the period after operation. no patient developed overt fungal infection but 15 patients developed precipitins to mannan antigens and 9 patients developed percipitins to cytoplasmic antigens during this period. the association b ... | 1979 | 120986 |
| present and future uses of mutagenicity tests for assessment of the safety of food additives. | the tier approach has been employed for the past 3 years for investigating the potential mutagenicity of food additives and generally recognized as safe (gras) substances. this approach is used to set testing priorities, since a large number of substances must be investigated. three tiers or testing levels are used. the first tier is a pre-screen to determine whether a chemical has the potential to cause genetic damage. the second tier is designed to ascertain whether mutagenic activity detected ... | 1977 | 121932 |
| defective blood mononuclear phagocyte function in patients with leprosy. | patients with lepromatous leprosy possess a defective lymphocyte function in vivo and in vitro that is less evident in the tuberculoid form. data concerning their macrophage ability to digest mycobacterium leprae are controversial. the purpose of this study was to determine whether monocytes from patients with either tuberculoid or lepromatous leprosy were altered in their enzyme systems, that is myeloperoxidase-dependent and myeloperoxidase-independent systems. the ability of adherent blood mon ... | 1979 | 122627 |
| investigations on the biological activity of extracts of tylopilus felleus (bull. ex fr.) p karst. by means of mycological tests. | | 1975 | 123071 |
| influence of spermine on some membrane-disturbing actions. | the influence of spermine on the potassium loss from yeast cells as a consequence of membrane-disturbing actions was investigated. spermine interfered strongly in the action membrane-active bactericides on yeast cells; this interference resembles the action of certain metal ions. spermine provides a protection against the positively charged parasosaniline and accomplishes a strong potentiation of the action of the negatively charged sodium dodecyl sulphate. | 1975 | 123389 |
| physiological and genetic modifications of the expression of the yeast mitochondrial adenosine triphosphatase inhibitor. | 1. the oligomycin-sensitive atpase activity of submitochondrial particles of the glycerol-grown "petite-negative" yeast: schizosaccharomyces pombe is markedly stimulated by incubation at 40 degrees c and by trypsin activations are treatment. both increased in triton-x 100 extracts of the submitochondrial particles. 2. a trypsin-sensitive inhibitory factor of mitochondrial atpase with properties similar to that of beef heart has been extracted and purified from glycerol-grown and glucose-grown s. ... | 1975 | 123768 |
| atpase inhibitor from yeast mitochondria. purification and properties. | 1. mitochondria from candida utilis cbs 1516 and sacchromyces cerevisiae jb 65 possess an atpase-inhibitor activity. the inhibitor activity depends on the growth conditions of the yeast cells. it is markedly decreased when the cells are grown in the presence of a high concentration of glucose, which suggests that glucose represses the synthesis of the atpase inhibitor or of a protein required for the insertion of the inhibitor into the inner mitochondrial membrane. 2. the atpase inhibitor has ... | 1975 | 123784 |
| 5-keto-d-fructose and its phosphate esters-1. | | 1975 | 124006 |
| [enzyme activity of yeasts as an indicator of their physiological state]. | during yeast cultivation the activity of hexokinase and phosphofructokinase, the triggering enzymes of glycolysis, was measured and the total amount of nucleic acids was determined. the impoverished medium--postdistillation molasses residue--was used for yeast generation in the studies. a decline in the enzyme activity was observed, wheras the total amount of nucleic acids remained unaltered. the activity of hexokinase and phosphofructokinase increased significantly when the yeast cultivated ... | 1975 | 124055 |
| phosphofructokinase of yeast. | | 1975 | 124388 |
| reconstitution of atp-32-pi exchange by phospholipid addition to the purified oligomycin-sensitive atpase from yeast mitochondria. | | 1975 | 124555 |
| purification and partial characterization of the oligomycin-sensitive atpase from yeast mitochondria. | | 1975 | 124556 |
| action of cytochalasin a, a sulfhydryl-reactive agent, on sugar metabolism and membrane-bound adenosine triphosphatase of yeast. | cytochalasin a at 10-20 mug/ml inhibits growth and sugar uptake by saccharomyces strain 1016. the effects of cytochalasin a in intact cells were completely prevented when 1 mm cysteine or dithiothreitol was added along with cytochalasin a, but were not eliminated by thiols added after inhibition had occurred. purified yeast hexokinase, glucose-6-p dehydrogenase, phosphofructokinase and aldolase were not sensitive to cytochalasin a (20 mug/ml). glyceraldehyde-3-p dehydrogenase was strongly inhibi ... | 1975 | 124588 |
| the mutagenicity of captan and related fungicides. | | 1975 | 124818 |
| mutagenicity of nitrofuran derivatives, including furylfuramide, a food preservative. | | 1975 | 124819 |
| proceedings: the effect of a watersoluble carbodiimide on plasma membrane atpase and cation transport in yeast. | | 1975 | 124821 |
| studies on the energy metabolism during anaerobic fermentation of glucose by baker's yeast. | as a result of the intimate association of adp phosphorylation with alcoholic fermentation, resulting in the synthesis of 2 mole atp per mole glucose fermented, it may be calculated that a minimum of 672 mucal heat development may be expected for every mm-3 co2 developed during alcoholic fermentation. when all atp produced would be fully de-phosphorylated to adp + pi (e.g. by atp-ase activity) a maximum heat development of 1200 mucal per mm-3 co2 could be expected. using the lkb-flow-microcalori ... | 1975 | 124899 |
| studies on energy-linked reactions: isolation, characterisation and genetic analysis of trialkyl-tin-resistant mutants of saccharomyces cerevisiae. | mutants of saccharomyces cerevisiae resistant to triethyl tin sulphate have been isolated and are cross-resistant to other trialkyl tin salts. triethyl-tin-resistant mutants fall into two general phenotypic classes: class 1 and class 2. class 1 mutants are cross-resistant to a variety of inhibitors and uncoupling agents which affect mitochondrial membranes (oligomycin, ossamycin, valinomycin, antimycin, erythromycin, chloramphenicol, '1799', tetrachlorotrifluoromethyl benzimidazole carbonylcyani ... | 1975 | 125200 |
| automated method for the determination of niacin and niacinamide in cereal products: collaborative study. | in a collaborative study, an automated method for the determination of niacin and niacinamide in cereal products was compared with the official final action microbiological (43.121-43.125) and chemical (43.044-43.046) methods. ten samples of cereal products, including enriched flour, yeast-leavened baked products, fortified breakfast cereals, and baked pet food products, were submitted to 14 laboratories. nine laboratories reported values by the automated method, 6 reported values by the microbi ... | 1975 | 125257 |
| mebendazole in the treatment of dracontiasis. | in a controlled field trial of treatment for dracontiasis, mebendazole (vermox) was found to be effective in eliminating the adult worm and preventing clinical relapses, but it did not achieve significant amelioration of symptoms, subsidence of inflammation, or healing of ulcers. the failure is attributed to poor absorption and lack of anti-inflammatory action of the drug. mebendazole was well tolerated and although two patients reported mild gastrointestinal disturbances, which may be attribute ... | 1975 | 125552 |
| the effect of thiols on saccharomyces fragilis. | treatment of cell suspensions of saccharomyces fragilis with 0.01 m beta-mercaptoethanol or dithiothreitol released a variety of substances of high and low molecular weight. twenty-two high-molecular-weight glycoproteins were separated by a combination of chromatography on deae cellulose and polyacrylamide gel electrophoresis in presence of sodium dodecylsulphate. the carbohydrate components consisted of at least 95% mannose and the protein components had threonine and serine as the major amino ... | 1975 | 125559 |
| allosteric activation and competitive inhibition of yeast phosphofructokinase by d-fructose. | purified phosphofructokinase from bakers yeast is activated by d-fructose in low concentrations (up to 1 mm) and inhibited by high concentrations. the stimulatory effect of d-fructose is similar, but smaller than that of amp. in the presence of amp (0.4 mm or higher) d-fructose does no longer stimulate, but its inhibitory effect persists (ki = 8 mm). its dualistic action on phosphofructokinase activity indicates that d-fructose might induce low frequency in glycolytic oscillations by direct inte ... | 1975 | 125565 |
| membrane-lipid unsaturation and mitochondrial function in saacharomyces cerevisiae. | the lipid composition of yeast cells was manipulated by the use of an unsaturated fatty acid auxotroph of saccharomyces cerevisiae. there was a 2-3-fold decrease in the concentration of cytochromes a+a3 when the unsaturated fatty acid content of the cells was decreased from 60-70% of the total fatty acid to 20-30%. the amounts of cytochromes b and c were also decreased under these conditions, but to a lesser extent. further lipid depletion, to proportions of less than 20% unsaturated fatty acid, ... | 1975 | 125585 |
| [preparation of synchronously growing and multiplying yeast cells (saccharomyces cerecisiae) (author's transl)]. | | 1975 | 125970 |
| [characterisation of rna from synchronously growing yeast cells (saccharomyces cerevisiae) as separated by columns of methylated albumin on kieselgur (mak) (author's transl)]. | total rna, extracted from synchronously growing yeast cells by the method of georgiev-mantieva, was separated by columns of methylated albumin on kieselgur (mak). by comparison with definited rna's in discelectrophorese and after pulse labelling with [3h]methionine the following fractions are identified: t-rna, 5.8s rna, m-rna, 18s and 25s r-rna and their precursors 20s, 27s and 35s. high molecular fractions of rna between 40s and 70s were also found. | 1975 | 125971 |
| purification and properties of the fatty acids synthetase complex from neurospora crassa, and the nature of the fas-mutation. | a procedure is described for the purification of the fatty acid synthetase complex (fas) from neurospora crassa. the enzyme complex has a molecular weight of 2.3 times 10(6), contains 6 mol of 4'-phosphopantetheine per mol, and on polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate gives a single band, or a closely spaced doublet, which comigrates with standard myosin (molecular weight, 2 times 10(5)). since the slightly retarded component in the doublet accounts for all ... | 1975 | 126228 |
| use of diazido ethidium bromide as a specific probe for mitochondrial functions. | the diazido derivative of ethidium bromide has been synthesized as a potential photoaffinity label and shown to be at least as effective as a mitochondrial mutagen as the parent compound, with a similar mode of action. exposure of mitochondria of saccharomyces cerevisiae to the compound, followed by ultraviolet-irradiation, which converts it to the highly reactive dinitrene, results in its specific binding to a single component which has been tentatively identified as the smallest polypeptide (s ... | 1975 | 126240 |
| [characterization of messenger ribonucleoproteins from yeasts (author's transl)]. | a method to produce in 15 min stable sphaeroplasts from yeast of the early log-phase is described. this method was used to isolate polysomes, after specific radioactive labelling of mrna. the mrnp *-particles, isolated from polysomes, have sedimentation-coefficients from 10 to 80s and a density of 1.4 g/cm3, the polysomes a density of 1.58 g/cm3 and the ribosomes a density of 1.6. g/cm3. | 1975 | 126593 |
| biogenesis of mitochondria. the effects of membrane unsaturated fatty acid content on the activity and assembly of the yeast mitochondrial protein-synthesizing system. | | 1975 | 126665 |
| characterization of 5.8s ribosomal ribonucleic acid in neurospora crassa. | neurospora crassa ribosomes contain a species of ribonucleic acid (rna) of molecular weight 54,000, similar to 5.8s ribosomal rna previously described for other eukaryotic organisms. the 5.8s rna from n. crassa was found to be released by heat treatment at 60 c from 25s ribosomal rna but not from 18s ribosomal rna. the base composition of n. crassa 5.8s rna was similar to that of 5.8s rna from saccharomyces cerevisiae, but differed from animal 5.8s rna. during the course of this study, it was di ... | 1975 | 126993 |
| anti-inflammatory activity of thiabendazole and its relation to parasitic disease. | in 6 differnet animal assays in the laboratory, thiabendazole had clear anti-inflammatory effect, though it was less potent than aspirin in all assays. these findings add support to clinical suggestions that the drug may have anti-inflammatory properties in man. such properties may contribute to the clinical response observed following the use of thiabendazole in cases of trichinosis, cutaneous larva migrans, visceral larva migrans, dracunculosis and scabies. in parasitic infections in which cor ... | 1975 | 127396 |
| [problem of interallelic complementation]. | | 1975 | 127479 |
| reaction of yeast phosphofructokinase with succinic and maleic anhydride. | modification of yeast phosphofructokinase by succinic and maleic anhydride influences the catalytic activity and the allosteric behaviour of the enzyme. depending on the degree of succinylation and maleinylation a decrease of maximum activity, an increase of the apparent affinity for fructose-6-phosphate, a decrease of the hill-coefficient and a diminution of atp-inhibition are observed. up to about 40% of the lysyl residues could be succinylated without dissociation of the hexameric protein, ho ... | 1975 | 127493 |
| regulation of mitochondrial biogenesis: enzymatic changes in cytochrome-deficient yeast mutants requiring delta-aminolevulinic acid. | yeast cells almost completely deficient in all cytochromes were obtained by introducing two defective nuclear genes, cyd1 and cyc4, into the same haploid strain. the action of the two mutant genes is synergistic, since either gene acting singly results in only partial cytochrome deficiency. normal synthesis of all cytochromes can be restored in the double mutant by adding delta-aminolevulinic acid to the growth medium. the optimum concentration of delta-aminolevulinate for restoration of cytochr ... | 1975 | 127792 |
| limited proteolysis of yeast phosphofructokinase by subtilisin. alterations in enzyme activity, subunit composition, and hydrodynamic properties. | yeast phosphofructokinase having a molecular weight of 750000--800000 (20 s) has been subjected to limited proteolysis by subtilisin and yeast proteases. two steps of proteolytic degradation could be distinguished: in the first step, which is accompanied by an increase in molecular activity, the subunits alpha and beta (mr 120000) are converted to alpha' and beta' (mr approximately 900000), and in the second step, accompanied by a decrease in enzyme activity, alpha' is converted to alpha'' (mr 8 ... | 1975 | 128452 |
| subunit structure of 6-phosphofructokinase from brewers' yeast. | an analysis of 6-phosphofructokinase from brewers' yeast in the presence of sodium dodecylsulfate reveals the occurrence of four components with the following molecular weights: alpha = 140000, beta = 130000, and alpha' = 92000, beta' = 87000. it was found that the alpha- and beta-components can be converted to the alpha' and beta' components by treatment of the native preparation with hyaluronidase. a comparison of the molecular weight obtained by ultracentrifugation and gel filtration with the ... | 1975 | 128516 |
| [genetic bases for the selection of microorganisms using analogs]. | the main mechanisms are considered for biosynthesis processes regulation by means of repression and inhibition with final products. on the basis of these mechanisms the state and prospects of the directed alteration of various stages in regulating the synthesis of primary and secondary metabolites, using breeding selection of mutants resistant to analogues are discussed. the main regularities are presented for using analogues of primary metabolites in the breeding strains with overproduction of ... | 1975 | 128859 |
| compartmentation and regulation of fungal metabolism: genetic approaches. | | 1975 | 129029 |
| biogenesis of mitochondria 36, the genetic and biochemical analysis of a mitochondrially determined cold sensitive oligomycin resistant mutant of saccharomyces cerevisiae with affected mitochondrial atpase assembly. | the isolation and characterisation of a mutant affecting the assembly of mitochondrial atpase is reported. the mutation confers resistance to oligomycin and venturicidin and sensitivity of growth on nonfermentable substrates to low temperature (19degrees). genetic analysis indicates that the phenotype is due to a single mutation located on the mitochondrial dna which is probably allelic with the independently isolated oligomycin resistance mutation [oli1-r]. growth of the mutant at the non-restr ... | 1975 | 129672 |
| an improved assay of uv-induced thymine-containing dimers in saccharomyces cerevisiae. | the method for assaying thymine-containing dimers in yeast is based on highly efficient ([3h]-deoxythymidine-5'-monophosphate) dna-specific labelling and employs ascending thin layer chromatography. it allows satisfactory quantitative analysis down to uv-doses of 500 erg/mm2. | 1975 | 130013 |
| uv-induction of thymine-containing dimers in saccharomyces cerevisiae. | in haploid and diploid s. cerevisiae the dimer yield ratio tt/ct is found to be 1.2/1 and 1.3/1, resp., at the uv (254 nm) unit dose 1 erg/mm2, the share of tt and ct in a uv (254 nm) lethal hit being 0.7 tt and 0.6 ct. a general formulation of the uv lethal hit is given and discussed. the tt+ct yields obtained for s. cerevisiae are compared to those reported for other organisms. it is found that there obviously exists a directly proportional linear correlation between genome size and tt+ct yiel ... | 1975 | 130014 |
| [radiobiological studies on the behavior of normal and tumorous plant tissues cultivated in vitro]. | vegetable tumoral tissue are more radiosensitive than the homologous normal tissue. tumoral tissue are more stimulated by yeast extract and yeast s-arn than normal tissue. yeast extracts and s-rna extracts from yeast also restore more the growth of tumorous tissue than normal tissue, which were subjected to gamma radiation from 60co. a mixture of mononucleotides obtained from fractionated s-rna has the same stimulatory effect. it is suggested that the active principle from yeast and s-rna is a c ... | 1975 | 130188 |
| specific binding of cibacron blue f3g-a to yeast phosphofructokinase. | the complex formation between the atp-analogous reactive dye cibacron blue f3g-a and yeast phosphofructokinase is accompanied by a red shift of the visible absorption spectrum. from the position of lambdamax of the dye-phosphofructokinase complex in the polarity scales obtained from model solvents it may be concluded that the chromophoric system is evidently located in a highly apolaric range of the enzyme protein. the spectrophotometric titration of yeast phosphofructokinase with cibacron blue ... | 1975 | 130771 |
| purification of protoplast-secreted acid phosphatase from baker's yeast. effect on adenosine triphosphatase activity. | in order to examine acid phosphatase (ec 3.1.3.2) and atpase (ec 3.6.1.3) activities of baker's yeast (ph optimum 3.5) a protoplast-secreted enzyme preparation was purified and some physical and chemical properties were studied. three protein fractions containing atpase and acid phosphatase activities, in the same ratio as the initial preparation, were separated by ion-exchange chromatography. the first fraction which had the highest protein content yielded a homogeneous preparation after sephar ... | 1976 | 130933 |
| lysine catabolism in rhizoctonia leguminicola and related fungi. | the catabolism of lysine was studied in several yeasts and fungi. results with cell-free extracts of rhizoctonia leguminicola support a proposed pathway involving (d- and l-) epsilon-n-acetyllysine, alpha-keto-epsilon-acetamidohexanoic acid, delta-acetamidovaleric acid, and delta-aminovaleric acid in the conversion of l-lysine to shortchain organic acids. label from radioactive l-lysine was found to accumulate in d- and l-epsilon-n-acetyllysine, delta-acetamidovaleric acid, delta-aminovaleric ac ... | 1976 | 131119 |
| development of mitochondrial membranes in anaerobically grown yeast cells. | biochemical analyses of mitochondrial marker substances, especially cardiolipin and oligomycin-sensitive atpase [ec 3.6.1.3], as well as electron microscopic observations were carried out to eludicate the process of mitochondrial development in annaerobic yeast cells. cardiolipin was found to be localized in the mitochondria in anaerobic cells. its cellular content was a little higher in the stationary phase than in the exponential phase in glucose-grown cells and increased further in galactose- ... | 1975 | 131794 |
| [size comparison of poly (a)-rna from polysomes and from nuclei of the yeast saccharomyces cerevisiae (author's transl)]. | the size of poly (a)-rna from polysomes and cell nuclei of the yeast saccharomyces cerevisiae was investigated. pulslabelled cells ([14c] adenin) were cracked by the french press; polysomes and nuclei were separately isolated and the rna was finally extracted with phenol. the separation of poly (a)-containing and poly (a)-lacking fractions was achieved by oligo (dt) cellulose. these fractions were characterized by sedimentation analysis. the main portion of polysomal poly (a)-rna sedimented with ... | 1976 | 132045 |
| mutagenicity assays on fluorescent whitening agents using microorganisms. | six fluorescent whitening agents (fwas) have been re-examined for their activity as inducers of cytoplasmic petite mutants and mitotic gene conversion in diploid yeast saccharomyces cerebisiae and reversion from auxotrophy to prototrophy in neurospora crassa, escherichia coli and salmonella typhimurium. the results provide no indication that the fwas examinded produce mutagenic changes or any other alterations in the gene material. in a recent re-examination with salmonella using the method of a ... | 1975 | 132347 |
| isolation and characterization of membranes from a hydrocarbon-oxidizing acinetobacter sp. | membranes were isolated and purified from nutrient broth-yeast extract- and hexadecane-grown cells of acinetobacter sp. strain ho1-n. two membrane fractions were isolated from nutrient broth-yeast extract-grown cells, the cytoplasmic membrane and the outer membrane. in addition to these two membrane fractions, a unique membrane fraction was isolated from hexadecane-grown cells (band 1) and characterized as a lipid-rich, low-density membrane containing high concentrations of hexadecane. the outer ... | 1976 | 132429 |
| studies on heart phosphofructokinase. use of fructose 6-sulfate as an alternative substrate to study the mechanism of action and active site specificity. | fructose 6-sulfate was synthesized by direct sulfurylation of fructose and was isolated by two selective steps: (a) conversion of the 6-sulfuryl ester to fructose 1-phosphate-6-sulfate with phosphofructokinase; (b) conversion of fructose 1-phosphate-6-sulfate to fructose 6-sulfate by fructose-1,6-diphosphatase. utilizing crystalline sheep heart phosphofructokinase, kinetic studies with the alternative substrate were carried out at ph 8.2 which is optimal for nonallosteric kinetics. the data are ... | 1976 | 132439 |
| evaluation and re-evaluation of genetic radiation hazards in man. i. interspecific comparison of estimates of mutation rates. | a detailed presentation is made of the experimental data from the various systems used by abrahamson et al. [2] to conclude that the per locus per rad (low let) radiation-induced forward mutation rates in organisms, whose dna content varies by a factor of about 1000, is proportional to genome size. additional information pertinent in this context is also reviewed. it is emphasized that the mutation rates cited by abrahamson et al. [2], although considered as pertaining to mutations at specific l ... | 1976 | 132611 |
| ziram, a sulfhydryl reagent and specific inhibitor of yeast mitochondrial dehydrogenases. | | 1976 | 132898 |
| oligomycin sensitivity of the mitochondrial atpase as a marker for fly transmissability and the presence of functional kinetoplast dna in african trypanosomes. | | 1976 | 133016 |
| [study of the incorporation of radioactive zinc in saccharomyces cerevisiae aldolase]. | saccharomyces cerevisiae aldolase concentrates from the culture medium containing znso4 a large amount of zn which becomes a component part of the enzyme molecular structure. this was made evident by adding to the culture medium 65znso4 and measuring the radioactivity of the aldolase extracted by a phillips single channel analyzer. | 1976 | 133445 |
| [study of purified aldolase from saccharomyces cerevisiae, using irradiated fructose-1,6-diphosphate]. | the interaction of an electromagnetic field with the enzymatic substrate-- the sodium salt of fructose-1,6-disphosphate--induces in the latter a new type of physical transition s leads to s. the enzyme, in this case saccharomyces cerevisiae aldolase, is able to reveal this new state of the substrate by an increase in its specific activity within well established irradiation times. each enzyme is characterized by the tm (minimal irradiation time of the substrate) a tau (fixed time period) paramet ... | 1976 | 133446 |
| analgesic and anti-inflammatory activity of 6-chloro-alpha-methyl-carbazole-2-acetic acid (c-5720). | the carbazole, c-5720, has the same order of analgesic, antipyretic and anti-inflammatory activity as indomethacin and is more potent than phenylbutazone and acetylsalicylic acid in the yeast inflamed paw, the carrageenin foot edema, the mycobacterium butyricum-induced pyrexia, and the acute and chronic adjuvant arthritis tests. in chronic adjuvant arthritis in rats, c-5720 lowers the elevated serum mucopolysaccharide and plasma fibrinogen levels, and partially restores the depressed liver n-dem ... | 1976 | 133645 |
| distribution of membranes, especially of plasma-membrane fragments, during zonal centrifugations of homogenates from glucose-repressed saccharomyces cerevisiae. | 1. the distributions of several enzymes and other marker components were examined after zonal centrifugations of whole homogenates from glucose-repressed saccharomyces cerevisiae on sucrose and iso-osmotic ficoll, and the composition and morphology of the fractions were investigated. 2. after high-speed zonal centrifugation most of the protein, acid and alkaline phosphatases, alkaline pyrophosphatase, adenosine monophosphatase, beta-fructofuranosidase, alpha-mannosidase, nadph-cytochrome c oxido ... | 1976 | 133674 |
| differences in the degradation of yeast phosphofructokinase by proteinases a and b from yeast. | proteinase a from yeast causes a stepwise degradation of yeast phosphofructokinase, resembling the action of subtilisin on this enzyme. proteinase b, however, exhibits a limited proteolysis similar to the action of alpha-chymotrypsin. | 1976 | 133676 |
| [permeability of yeast mitochondrial internal membrane: structure-activity relationship]. | in order to investigate the possible relations between the anionic permeability and the functions (or the structure ) of the inner mitochondrial membrane, three types of organelles isolated from s. cerevisiae were tested: mitochondria (aerobic culture), promitochondria (anaerobic culture) and cap-mitochondria (aerobic culture with chloramphenicol added). by using the technique of swelling in isoosmotic potassium salts, after a derermination of the isotonic conditions, it was possible to discrimi ... | 1976 | 133731 |
| assembly of the mitochondrial membrane system xvi. modified form of the atpase proteolipid in oligomycin-resistant mutants of saccharomyces cerevisiae. | | 1976 | 133820 |
| inhibition of neurospora crassa and yeast mitochondrial protein synthesis by ricin, a toxic protein inactive on e. coli protein synthesis. | | 1976 | 133821 |
| action of intracellular proteinases on mitochondrial translation products of neurospora crassa schizosaccharomyces pombe. | gel electrophoretic analysis of mitochondrial membranes from neurospora crassa shows the presence of a polypeptide fraction with apparent molecular weights of 7000 - 1200, which is synthesized on mitochondrial ribosomes. this fraction comprises between 10 and 50% of total mitochondrial translation products. evidence is presented that the major part of this fraction is derived from components with higher apparent molecular weights by proteolytic activity. the proteolytic activity is located in ve ... | 1976 | 133982 |
| genetic analysis of mitochondrial biogenesis and function in saccharomyces cerevisiae. | different mitochondrial mutants have been isolated that affect mitochondrial ribosome function. these mutants were used to establish most of the known methods and principles of mitochondrial genetics in yeast. another class of mitochondrial mutants have been shown to affect mitochondrial atpase and, more specifically, the "membrane factor" of mitochondrial atpase. these mutants might be very useful in studying the energy-conserving function, and the interaction between the hydrophobic and hydrop ... | 1976 | 134034 |
| atmospheric mutagens. i. sulfur oxides and nitrogen oxides. | | 1976 | 134284 |
| fractionated precipitation of acid macropolyanions by dialysis, a simple method for the estimation of dna in complex biological samples. | after efficient extraction by para-aminosalicylate, chopping, grinding and eventual sonication, the macropolyanions are transformed into their cetyltrimethylammonium salts. these have differing solubilities, strongly depending on ionic strength. the cationic detergent-macropolyanionic salts are solubilized by high salt concentration. salt is then dialysed out, rendering the polyanions highly insoluble in a sequential fashion. the insolubilized components are determined quantitatively by monitori ... | 1976 | 134556 |
| [initiation in a polyribosome-dependent protein-synthesizing cell-free system from saccharomyces (author's transl)]. | a method to prepare polyribosomes from yeasts by using the french-press is described. the highest yield of polyribosomes was derived from late log-phase cells. these polyribosomes, incubated in a cell-free system, were able to reinitiate protein synthesis, which was shown by inhibiting aminoacid incorporation by aurintricarboxylic acid, edeine and sodiumfluoride. we developed the translational system in order to look for the optimal ion-conditions of a dna-dependent protein-synthesizing system. ... | 1976 | 134559 |
| [effect of n-methyl-n-beta-chloroethyl-hydrazine-hcl on the growth and multiplication of yeast cells (saccharomyces cerevisiae) (author's transl)]. | n-methyl-n-beta-chloroethyl-hydrazine-hcl and its benzaldehydhydrazone inhibit growth and multiplication of yeast cells. the dna-synthesis is partially blocked. after removal of the substances the rate of this synthesis is much more increased than that of rna and protein. the both substances we tested are more effective than simple alkylhydrazines. | 1976 | 134572 |
| [effect of n-methyl-n-beta-chloroethylhydrazine and its benzaldehydhydrazone on rna-and protein synthesis as well as metabolism of synchronously growing yeast cells (saccharomyces cerevisiae) (author's transl)]. | n-methyl-n-beta-chloroethylhydrazine and its benzaldehydhydrazone inhibit the influx of [6-3h]-uracil and l-[u-14c]leucin in yeast cells as well as the incorporation in rna and protein. experiments with synchronously and asynchronously growing cells showed the n-methyl-n-beta-chloroethylhydrazine essentially influences the dividing phase while the benzaldehydhydrazone is more effective in the growth phase. the effect of these two substances on respiration, glycolysis and the concentration of fru ... | 1976 | 134573 |
| [distribution of [3h] thiopyronine in yeast cells (saccharomyces cerevisiae)]. | | 1976 | 134587 |
| studies on the oligomeric structure of yeast phosphofructo-kinase by means of cross-linking diimidoesters. | | 1976 | 134707 |
| localization on mitochondrial dna of mutations leading to a loss of rutamycin-sensitive adenosine triphosphatase. | four cytoplasmic mutants of saccharomyces cerevisiae showing loss of mitochondrial rutamycin-sensitive atpase activity but having significant cytochrome oxidase and nadh-cytochrome c reductase have been isolated. genetic studies indicate the mutations to be closely linked to each other and have been assigned to a new locus, pho1. the mutations show a low frequency of recombination with the ol12 locus, suggesting a linkage to this marker. they are not, however, linked to the oli1 locus. linkage o ... | 1976 | 134892 |
| a low-molecular-weight atpase from wheat-seedling mitochondria. | an atpase which strikingly differed from the mitochondrial atpases of yeast and of animal tissues was obtained when wheat seedling mitochondria, or electron transport particles derived from them, were subjected to ultrasonication and treated with ammonium sulphate. the enzyme which was purified by chromatography on sephadex g-100 and deae-sephadex (a50) failed to be inactivated as low as 43 000. the enzyme preparation was capable of hydrolysing adp, in addition to atp, and several other nucleosi ... | 1976 | 134893 |
| [value of a therapeutic combination: vitamin a-yeast-sulfur]. | | 1976 | 135073 |
| [dimorphism in "ustilago cynodontis". ii--glucidic metabolism (author's transl)]. | the glucidic metabolism has been studied in four strains of ustilago cynodontis. two of them--m1 and m7--are mycelial strains, the two others --l1 and l7--being yeast like are respectively issued from m1 and m7. the results obtained show that the choice between the different glucidic catabolism pathways takes place at the phosphofructokinase level. when the phosphofructokinase is lacking (m1) the catabolism occurs via the pentose phosphate cycle followed by the last glycolytic reactions (between ... | 1976 | 135525 |
| energy-linked activities in reconstituted yeast adenosine triphosphatase proteoliposome. adenosine triphosphate formation coupled with electron flow between ascorbate and ferricyanide. | | 1976 | 135528 |
| studies of 1-deoxy-d-fructose, 1-deoxy-d-glucitol, and 1-deoxy-d-minnitol as antimetabolites. | 1-deoxy-d-fructose was synthesized in 27% yield from d-glucosamine in a three-step procedure involving raney nickel desulfurization and oxidative deamination with 3,5-di-tert-butyl- 1,2-benzoquinone applied to appropriate intermediates. 1-deoxyfructose and its reduction products, 1-deoxyglucitol and 1-deoxymannitol, were tested as substrates and antimetabolites. for sheep liver glucitol dehydrogenase, the km is 53 mm for 1-deoxymannitol, were tested as substrates and antimetabolites. for sheep l ... | 1976 | 135580 |
| the control of cell membrane fluidity by biochemical and catalytic hydrogenation processes. | | 1976 | 136314 |
| [trichosporon capitatum as pathogen of dermatomycoses]. | three cases of dermatomcycosis are reported, caused by trichosporon capitatum, a yeast-like organism, belonging to genus trichosporon of the cryptococcaceae, which till now was isolated out of dermatological material only in a few cases. one of the lesions, characterized as "leuconychia mycotica", is a problematic task with regard to the treatment, for it is a professional infection in an offset-printer. a glycerol containing fluid for wiping his printing machine was detected as reservoir of yea ... | 1976 | 136429 |
| subunit composition and biogenesis of mitochondrial cytochrome b. | | 1976 | 136995 |
| mitochondrial adenosine triphosphatase from yeast, saccharomyces cerevisiae. purification, subunit structure and kinetics. | 1. a procedure for the purification of atpase extracted by chloroform from baker's yeast (saccharomyces cerevisiae) is reported. the yield based on submitochondrial particles was 55% and the purification was 100-fold. the isolated complex was homogenous as assessed by gel filtration, ion-exchange chromatography, sedimentation in sucrose gradient and in the analytical ultracentrifuge. the molecular weight determined by gel filtration was 400000 +/- 20000. ultracentrifugation yielded s020,w = 12.5 ... | 1976 | 137202 |
| [atp content in candida tropicalis cells growing at different temperatures]. | the thermotolerant yeast candida tropicalis, strain t-20, was cultivated on a chemically defined medium with glucose or malt wort in flasks with shaking at three temperatures: optimal (36degreesc), supraoptimal (38degreesc) and submaximal (41degreesc). an increase of temperature within these limits caused an increase in atp content in yeast cells and a decrease in phosphohydrolase (atpase) activity. | 1976 | 137353 |
| a simple method for the large-scale preparation of mitochondria from microorganisms. | | 1977 | 137681 |
| a protein inhibitor of mitochondrial adenosine triphosphatase (f1) from saccharomyces cerevisiae. | a heat-stable protein has been detected in saccharomyces cerevisiae which inhibits mitochondrial atpase activity. the protein inhibitor has been isolated from extracts prepared by brief heat treatment of unbroken cell suspensions. the isolated inhibitor is a small basic protein (molecular weight close to 7000, isoelectric proint 9.05) devoid of tryptophan, tyrosine, and cysteine as well as proline. the nhp2-terminal amino acid is serine. the ultraviolet absorption spectrum shows the vibrational ... | 1977 | 137903 |
| distribution of plasma-membrane fragments during zonal centrifugations of homogenates from aerobic saccharomyces cerevisiae. | | 1977 | 137959 |
| a cytoplasmic gene for partial suppression of a nuclear pleiotropic respiratory deficient mutant in the petite negative yeast schizosaccharomyces pombe. | the nuclear pleiotropic respiratory-deficient mutant pet1 (previously m126) exhibits cytochromes aa3 and b deficiencies accompanied by loss of the oligomycin-sensitivity of the mitochondrial atpase. the mutant pet1, unable to grow on glycerol, growth on glucose. the latter phenotypic trait symbolized by anas-d, exhibits a high frequency (2 to 4 x 10(5)) of spontaneous suppression into antimycin a-resistant strains. mutagenesis with mncl2 increases by a factor of 10(2) the frequency of anar-d der ... | 1976 | 138078 |
| mutation induction by the antischistosomal drug f30066 in various test systems. | the genetic activity of furapromidium (f30066), an antischistosomal drug, was studied in salmonella typhimurium, saccharomyces cerevisiae, neurospora crassa and cultured chinese hamster cells. the results show that f30066 induces gene mutations in s. typhimurium, n. crassa and chinese hamster cells. this compound also causes gene conversions in s. cerevisiae. | 1977 | 138086 |
| [experimental investigations on ultraweak photonemission form biological systems (author's transl)]. | measurements on untreated yeast cells, potato- and cucumber seedlings have confirmed the existence of photon emission from biological systems known as "ultraweak luminescence". the intensity is of the order of 10(2) counts per second, and the spectral distrubtion shows a maximum near 550 nm. the dependence of th photon emission on certain chemicals has been investigated. some chemicals, as for instance acetone, intensify the photon emission from cucumber seedlings without essential change of the ... | 1976 | 138296 |
| on the role of enzyme cooperativity in metabolic oscillations: analysis of the hill coefficient in a model for glycolytic periodicities. | the role of enzyme cooperativity in the mechanism of metabolic oscillations is analyzed in a concerted allosteric model for the phosphofructokinase reaction. this model of a dimer enzyme activated by the reaction product accounts quantitatively for glycolytic periodicities observed in yeast and muscle. the hill coefficient characteristic of enzyme-substrate interactions is determined in the model, both at the steady state and in the course of sustained oscillations. positive cooperativity is a p ... | 1976 | 138454 |
| an allergist looks at urticaria. | some etiologic possibilities which must be considered are foods, drugs, infection, inhalant sensitivity, psychic factors, physical agents, underlying connective tissue disease or neoplasm, insect bite or stings and genetic abnormalities. a painstaking history and a complete physical examination are, of course, mandatory. these are followed by appropriate studies for whatever etiologic factors are suggested by the history and physical examination. certain routine or more sophisticated studies mig ... | 1976 | 138566 |
| mucidin resistance in yeast. isolation, characterization and genetic analysis of nuclear and mitochondrial mucidin-resistant mutants of saccharomyces cerevisiae. | mutants of saccharomyces cerevisiae resistant to the antibiotic mucidin, a specific inhibitor of electron transport between cytochrome b and c, were isolated and divided into three phenotypic groups, as follows. class 1 mutants were cross-resistant to a variety of mitochondrial inhibitors and exhibited no resistance at the mitochondrial level. class 2 mutants were specifically resistant to mucidin exhibiting resistance also at the level of isolated mitochondria. biochemical studies indicated tha ... | 1977 | 138589 |
| the effect of temperature change on the kinetic behaviour of yeast phosphofructokinase. | yeast phosphofructokinase does not exhibit any cold sensitivity. the kinetic properties of the enzyme have been investigated in the range between 10 degrees c and 30 degrees c in dependence on fructose 6-phosphate and atp. although a significant increase in the enzyme activity with rising temperature does not occur, the shape of the atp velocity curves is not markedly altered. with increasing concentrations of fructose-6-phosphate the efficiency of temperature on the catalytic process increases, ... | 1976 | 139067 |