| identification of a novel highly conserved gene in the centromeric part of the major histocompatibility complex. | a novel highly conserved gene designated sacm2l alias are1) has been identified and fine mapped in the centromeric part of the major histocompatibility complex in rat, human, and mouse. sacm2l is closely linked to the ribosomal protein s18 gene rps18 with a distance of about 450 bp between the respective translational start points. numerous sacm2l-homologous est sequences can be identified in the database. northern blot experiments of rat sacm2l revealed a transcript of 3 kb in each organ tested ... | 1998 | 9790748 |
| cortactin associates with the cell-cell junction protein zo-1 in both drosophila and mouse. | cortactin is an actin filament-binding protein localizing at cortical regions of cells and a prominent substrate for src family protein-tyrosine kinases in response to multiple extracellular stimuli. human cortactin has been identified as a protein product of a putative oncogene, ems1. in this report, we describe the identification of a drosophila homolog of cortactin as a molecule that interacts with drosophila zo-1 using yeast two-hybrid screening. drosophila cortactin is a 559-amino acid prot ... | 1998 | 9792678 |
| adenovirus-mediated expression of a dominant negative mutant of p65/rela inhibits proinflammatory gene expression in endothelial cells without sensitizing to apoptosis. | we hypothesized that blocking the induction of proinflammatory genes associated with endothelial cell (ec) activation, by inhibiting the transcription factor nuclear factor kappab (nf-kappab), would prolong survival of vascularized xenografts. our previous studies have shown that inhibition of nf-kappab by adenovirus-mediated overexpression of i kappab alpha suppresses the induction of proinflammatory genes in ec. however, i kappab alpha sensitizes ec to tnf-alpha-mediated apoptosis, presumably ... | 1998 | 9794384 |
| yac rescue of downless locus mutations in mice. | mice with mutations at the downless (dl) locus have defects in hair follicle, tooth, sweat gland, preputial gland, meibomian gland, and tail development. the dl phenotype is analogous to the human genetic disorder termed autosomal hypohidrotic (or anhidrotic) ectodermal dysplasia (hed). on the basis of the identification of two related transgenic insertional mutations in the downless gene, yeast artificial chromosomes (yacs) were identified that map to the critical region of mouse chromosome (ch ... | 1998 | 9799834 |
| homology between human chromosome 2p13.3 and the wobbler critical region on mouse chromosome 11: comparative high-resolution mapping of sts and est loci on yac/bac contigs. | human chr 2p13-14 and homologous regions on mouse chrs 6 and 11 have been subjects of previous studies because they comprise the loci for several neuromuscular diseases. here we report on high-resolution mapping of 55 sts and est loci on human chr 2p13.3 and of 47 markers on the corresponding region on proximal mouse chr. 11. the maps comprise several known genes, meis1/meis1, rab1a/rab1a, mdh1/mor2, otx1/otx1, and rel on human 2p13.3 and mouse chr 11, respectively, as well as the wobbler (wr) c ... | 1998 | 9799840 |
| cloning and characterization of human sep1 (hsep1) gene and cytoplasmic localization of its product. | we isolated and sequenced a human cdna (designated as hsep1) encoding both a homologue of mouse dhm2 and budding yeast sep1. the gene was shown to be located on the long arm of chromosome 3 (3q25-26.1). the putative hsep1 product (hsep1p) consisted of 1694 amino acid residues with a molecular mass of about 190 kda. northern blot analysis showed a major 10-kb mrna expressed ubiquitously in various organs as well as a minor 5.5-kb mrna expressed relatively highly in the testis and placenta. hsep1p ... | 1998 | 9802570 |
| expression and regulation of mrna for putative fatty acid transport related proteins and fatty acyl coa synthase in murine epidermis and cultured human keratinocytes. | the epidermis has a requirement for fatty acids in order to synthesize cellular membranes and the extracellular lipid lamellar membranes in the stratum corneum. despite high endogenous production of fatty acids the transport of exogenous essential fatty acids into the epidermis is an absolute requirement. fatty acid uptake by keratinocytes has been shown to be mediated by a transport system. in this study we determined in murine epidermis and human cultured keratinocytes the expression of three ... | 1998 | 9804328 |
| high-resolution genetic, physical, and transcript map of the mnd2 region of mouse chromosome 6. | the autosomal recessive mutation mnd2 is responsible for a lethal neuromuscular wasting disorder in the mouse. a high-resolution genetic map of the mnd2 region of mouse chromosome 6 was generated by analysis of 1147 f2 offspring from an intersubspecific cross between strains c57bl/6j-mnd2/+ and cast/ei. the results localize mnd2 to the 0.2-cm interval between d6mit164 and d6mit128. a contig of overlapping yac, bac, and p1 clones spanning the nonrecombinant interval was constructed. one novel gen ... | 1998 | 9806835 |
| recently identified molecular aspects of intestinal iron absorption. | gene mapping techniques to identify gene mutations in humans and animals with phenotypic abnormalities in iron metabolism are providing important insights into the probable molecular mediators of intestinal iron absorption. positional gene cloning in humans with hereditary hemochromatosis has identified a mutation in a novel major histocompatibility complex (mhc) gene called hfe that is likely to be involved in regulating intestinal iron absorption. in addition, recent observations based on posi ... | 1998 | 9808632 |
| protein kinase profile of sperm and eggs: cloning and characterization of two novel testis-specific protein kinases (aie1, aie2) related to yeast and fly chromosome segregation regulators. | we have analyzed the general protein kinase expression profile in mouse sperm and eggs. a total of 41 different kinases were identified. in this study, we describe two novel protein kinases, designated aie1 (mouse) and aie2 (human), which share high amino acid identities with the serine/threonine (s/t) kinase domain of yeast ip11, fly aurora, and frog eg2. mutations in ip11 and aurora have been reported to cause abnormal chromosome segregation and centrosome separation. both aie1 and aie2 contai ... | 1998 | 9809744 |
| when overexpressed, a novel centrosomal protein, ranbpm, causes ectopic microtubule nucleation similar to gamma-tubulin. | a novel human protein with a molecular mass of 55 kd, designated ranbpm, was isolated with the two-hybrid method using ran as a bait. mouse and hamster ranbpm possessed a polypeptide identical to the human one. furthermore, saccharomyces cerevisiae was found to have a gene, ygl227w, the cooh-terminal half of which is 30% identical to ranbpm. anti-ranbpm antibodies revealed that ranbpm was localized within the centrosome throughout the cell cycle. overexpression of ranbpm produced multiple spots ... | 1998 | 9817760 |
| repression by the mad(mxi1)-sin3 complex. | the functions of myc in transformation and transactivation are countered by the suppressive actions of the mad(mxi1) family. mad(mxi1) proteins not only compete with myc for dimerization to max and binding to myc/max consensus sites but also recruit powerful repressors of gene expression. a prediction of the yin-yang relationship between myc and mad(mxi1) families would be that the latter constitutes a new class of tumor suppressors. here, we review the current literature on the mad(mxi1) family ... | 1998 | 9819568 |
| human carbon catabolite repressor protein (ccr4)-associative factor 1: cloning, expression and characterization of its interaction with the b-cell translocation protein btg1. | the human btg1 protein is thought to be a potential tumour suppressor because its overexpression inhibits nih 3t3 cell proliferation. however, little is known about how btg1 exerts its anti-proliferative activity. in this study, we used the yeast 'two-hybrid' system to screen for interacting protein partners and identified human carbon catabolite repressor protein (ccr4)-associative factor 1 (hcaf-1), a homologue of mouse caf-1 (mcaf-1) and saccharomyces cerevisiae ycaf-1/pop2. in vitro the hcaf ... | 1998 | 9820826 |
| recognition specificity of individual eh domains of mammals and yeast. | the eps homology (eh) domain is a recently described protein binding module that is found, in multiple or single copies, in several proteins in species as diverse as human and yeast. in this work, we have investigated the molecular details of recognition specificity mediated by this domain family by characterizing the peptide-binding preference of 11 different eh domains from mammal and yeast proteins. ten of the eleven eh domains could bind at least some peptides containing an asn-pro-phe (npf) ... | 1998 | 9822599 |
| rescue of the embryonic lethal hematopoietic defect reveals a critical role for gata-2 in urogenital development. | mutations resulting in embryonic or early postnatal lethality could mask the activities of any gene in unrelated and temporally distinct developmental pathways. targeted inactivation of the transcription factor gata-2 gene leads to mid-gestational death as a consequence of hematopoietic failure. we show here that a 250 kbp gata-2 yeast artificial chromosome (yac) is expressed strongly in both the primitive and definitive hematopoietic compartments, while two smaller yacs are not. this largest ya ... | 1998 | 9822612 |
| a functional homolog of a yeast trna splicing enzyme is conserved in higher eukaryotes and in escherichia coli. | trna splicing in the yeast saccharomyces cerevisiae requires an endonuclease to excise the intron, trna ligase to join the trna half-molecules, and 2'-phosphotransferase to transfer the splice junction 2'-phosphate from ligated trna to nad, producing adp ribose 1"-2" cyclic phosphate (appr>p). we show here that functional 2'-phosphotransferases are found throughout eukaryotes, occurring in two widely divergent yeasts (candida albicans and schizosaccharomyces pombe), a plant (arabidopsis thaliana ... | 1998 | 9826666 |
| genomic organization of a 225-kb region in xq28 containing the gene for x-linked myotubular myopathy (mtm1) and a related gene (mtmr1). | mtm1 is responsible for x-linked recessive myotubular myopathy, which is a congenital muscle disorder linked to xq28. mtm1 is highly conserved from yeast to humans. a number of related genes also exist. the mtm1 gene family contains a consensus sequence consisting of the active enzyme site of protein tyrosine phosphatases (ptps), suggesting that they belong to a new family of ptps. database searches revealed homology of myotubularin and all related peptides to the cisplatin resistance-associated ... | 1998 | 9828128 |
| regulation of insulin-stimulated glut4 translocation by munc18c in 3t3l1 adipocytes. | insulin stimulates glucose transporter (glut) 4 vesicle translocation from intracellular storage sites to the plasma membrane in 3t3l1 adipocytes through a vamp2- and syntaxin 4-dependent mechanism. we have observed that munc18c, a mammalian homolog of the yeast syntaxin-binding protein n-sec1p, competed for the binding of vamp2 to syntaxin 4. consistent with an inhibitory function for munc18c, expression of munc18c, but not the related munc18b isoform, prevented the insulin stimulation of glut4 ... | 1998 | 9837979 |
| tumor specific boosting of il-2 induced nk activation by paraformaldehyde fixed tumor cells. | nk activation in c57b1/6 mouse spleen cells was carried out with il-2 in the presence or absence of paraformaldehyde fixed yac tumor cells. generation of anti-yac cytolytic activity was markedly higher when activation was carried out in the presence of fixed tumor cells. in addition the cytotoxic effector cells generated were resistant to anti-thy-1 + c treatment, indicating that the effector cells were not t lymphocytes. il-2 activation of nk cells was compared when fixed yac or el4 tumor cells ... | 1998 | 9840684 |
| mitop: database for mitochondria-related proteins, genes and diseases. | the mitop database http://websvr.mips.biochem.mpg. de/proj/medgen/mitop/ consolidates information on both nuclear- and mitochondrial-encoded genes and their proteins. the five species files- saccharomyces cerevisiae, mus musculus, caenorhabditis elegans, neurospora crassa and homo sapiens -include annotated data derived from a variety of online resources and the literature. a wide spectrum of search facilities is given in the interelated sections 'gene catalogues', 'protein catalogues', 'homolog ... | 1999 | 9847163 |
| interaction of bp180 (type xvii collagen) and alpha6 integrin is necessary for stabilization of hemidesmosome structure. | the hemidesmosome is a multimolecular complex that integrates the extracellular matrix with the keratin cytoskeleton and that stabilizes epithelial attachment to connective tissue. a 180 kda protein (bp180, type xvii collagen), first identified by its reactivity with autoantibodies in the serum of patients with a blistering skin disease called bullous pemphigoid (bp), is a transmembrane component of the hemidesmosome with a collagen-like extracellular domain. here, using recombinantly expressed ... | 1998 | 9856810 |
| identification of strap, a novel wd domain protein in transforming growth factor-beta signaling. | transforming growth factor-beta1 (tgf-beta1) is the prototype of a large family of proteins that regulate a variety of biological processes. the pleiotropic responses to tgf-beta are mediated via ligand-induced heteromeric complex formation by type i (tbetar-i) and type ii (tbetar-ii) serine-threonine kinase receptors. several studies have shown that tbetar-ii acts as a primary receptor, binding tgf-beta and phosphorylating tbetar-i, whose kinase activity then propagates the signals. therefore, ... | 1998 | 9856985 |
| alternative splicing results in differential expression, activity, and localization of the two forms of arginyl-trna-protein transferase, a component of the n-end rule pathway. | the n-end rule relates the in vivo half-life of a protein to the identity of its n-terminal residue. the underlying ubiquitin-dependent proteolytic system, called the n-end rule pathway, is organized hierarchically: n-terminal aspartate and glutamate (and also cysteine in metazoans) are secondary destabilizing residues, in that they function through their conjugation, by arginyl-trna-protein transferase (r-transferase), to arginine, a primary destabilizing residue. we isolated cdna encoding the ... | 1999 | 9858543 |
| identification and characterization of stk12/aik2: a human gene related to aurora of drosophila and yeast ipl1. | mutations in aurora of drosophila and related saccharomyces cerevisiae ipl1 protein kinases are known to cause abnormal chromosome segregation. we earlier isolated a cdna encoding a novel human protein kinase aik which shares high amino acid identity with the aurora/ipl1 protein kinase family. in the present study, a second human cdna highly homologous to aurora/ipl1 (aik2) was identified and the nucleotide sequence was determined (gene symbol stk12). the c-terminal kinase domain of the stk12 en ... | 1998 | 9858806 |
| assignment of galgt encoding beta-1, 4n-acetylgalactosaminyl-transferase (galnac-t) and kif5a encoding neuronal kinesin (d12s1889) to human chromosome band 12q13 by assignment to ici yac 26eg10 and in situ hybridization. medjph@stjames.leeds.ac.uk. | | 1998 | 9858832 |
| mint 3: a ubiquitous mint isoform that does not bind to munc18-1 or -2. | mint 1 and 2 are proteins that bind to munc18-1, an essential component of the synaptic vesicle fusion machinery, and are detectably expressed only in neurons [okamoto and südhof, j. biol. chem. 272, 31459-31464 (1997)]. mint 1 and 2 are composed of a variable n-terminal region that includes a conserved munc18-1-binding site, and a constant c-terminal region that contains one ptb and two pdz domains. we have now identified a third mint isoform, mint 3. similar to mint 1 and 2, the c-terminal hal ... | 1998 | 9860131 |
| isolation of growth suppressors from a cdna expression library. | we describe an experimental procedure for the isolation of growth inhibitory sequences from a complex cdna library. this approach first takes advantage of the setgap technique (selectable expression of transient growth arrest phenotype) to enrich for growth inhibitory sequences, followed by a screening procedure to identify individual cdnas that inhibit cell proliferation. here we provide a detailed description of the experimental protocol and report the characterization of two cdna sequences is ... | 1998 | 9872334 |
| characterization of the mouse src homology 3 domain gene sh3d2c on chr 7 demonstrates coexpression with huntingtin in the brain and identifies the processed pseudogene sh3d2c-ps1 on chr 2. | formation of intracellular protein complexes is often mediated by src homology 3 domain-containing proteins interacting with proline-rich target sequences on other proteins. the sh3d2c gene or its rat/human orthologs have been implicated in synaptic vesicle recycling due to interaction with dynamin i and synaptojanin in nerve terminals. in a yeast two-hybrid system, association with a huntingtin fragment containing an elongated stretch of polyglutamines was observed recently. by genetic mapping ... | 1998 | 9878254 |
| partial rescue of gata-3 by yeast artificial chromosome transgenes. | gata-3 is essential for murine embryonic development, but elucidating the genetic controls over the complex temporal and tissue-specific transcriptional regulatory pattern of this transcription factor gene has been problematic. here we report the isolation and characterization of two yeast artificial chromosomes (yacs) bearing the murine gata-3 gene. ordered deletions of both yacs show that they define a 1-megabase pair contig spanning the gata-3 locus. we found that a 120-kb yac transgene, incl ... | 1998 | 9882482 |
| vinexin: a novel vinculin-binding protein with multiple sh3 domains enhances actin cytoskeletal organization. | using the yeast two-hybrid system and an in vitro binding assay, we have identified a novel protein termed vinexin as a vinculin-binding protein. by northern blotting, we identified two types of vinexin mrna that were 3 and 2 kb in length. screening for full-length cdna clones and sequencing indicated that the two mrna encode 82- and 37-kd polypeptides termed vinexin alpha and beta, respectively. both forms of vinexin share a common carboxyl-terminal sequence containing three sh3 domains. the la ... | 1999 | 9885244 |
| molecular mapping of chromosome 2 deletions in murine radiation-induced aml localizes a putative tumor suppressor gene to a 1.0 cm region homologous to human chromosome segment 11p11-12. | radiation-induced acute myeloid leukemias (amls) in the mouse are characterized by chromosome 2 deletions. previous studies showed that a minimal deleted region (mdr) of approximately 6.5 cm is lost from one homologue in chromosome 2-deleted amls. an aml tumor suppressor gene is proposed to map within this mdr. in this study, we refine the mdr to a i cm interval between markers d2mit126 and d2mit185 by microsatellite analysis of 21 primary radiation-induced f i amls. the construction of a partia ... | 1999 | 9885975 |
| localization of distant urogenital system-, central nervous system-, and endocardium-specific transcriptional regulatory elements in the gata-3 locus. | we found previously that neither a 6-kbp promoter fragment nor even a 120-kbp yeast artificial chromosome (yac) containing the whole gata-3 gene was sufficient to recapitulate its full transcription pattern during embryonic development in transgenic mice. in an attempt to further identify tissue-specific regulatory elements modulating the dynamic embryonic pattern of the gata-3 gene, we have examined the expression of two much larger (540- and 625-kbp) gata-3 yacs in transgenic animals. a lacz r ... | 1999 | 9891089 |
| zebrafish yac, bac, and pac genomic libraries. | numerous positional cloning projects directed at isolating genes responsible for the myriads of observed developmental defects in the zebrafish are anticipated in the very near future. in this chapter, we have reviewed the yac, bac, and pac large-insert genomic resources available to the zebrafish community. we have discussed how these resources are screened and used in a positional cloning scheme and have pointed out frequently formidable logistical considerations in the approach. despite being ... | 1999 | 9891341 |
| high-level expression of human interleukin-17 in the yeast pichia pastoris. | human interleukin-17 (hil-17) gene without the signal sequence was isolated from activated peripheral blood lymphocytes by rt-pcr, then highly expressed in the yeast pichia pastoris in the form of the glycosylated monomer. the monomer of rhil-17 stimulated mouse fibroblast 3t3 cells to secrete il-6 and was specifically bound to its receptors on 3t3 cells. | 1998 | 9891843 |
| characterization of mouse ubiquitin-like smt3a and smt3b cdnas and gene/pseudogenes. | mouse smt3a and smt3b cdnas encoding ubiquitin-like proteins of 110 and 95 amino acids, respectively, were isolated and sequenced. the sequence of the first 92 amino acids (ending with the conserved gly-gly) of mouse smt3a exhibited two differences at amino acid no. 38 and 76 in comparison with that of human smt3a. the c-terminal 18 amino acid sequence of mouse smt3a was completely different from the c-terminal 11 amino acid sequence of human smt3a. mouse and human smt3b were identical for a seq ... | 1998 | 9891849 |
| analysis of a conditional degradation signal in yeast and mammalian cells. | the n-end rule pathway is a ubiquitin-dependent proteolytic system, the targets of which include proteins that bear destabilizing n-terminal residues. the latter are a part of the degradation signal called the n-degron. arg-dhfrts, an engineered n-end rule substrate, bears n-terminal arginine (a destabilizing residue) and dhfrts [a temperature-sensitive mouse dihydrofolate reductase (dhfr) moiety]. previous work has shown that arg-dhfrts is long-lived at 23 degreesc but short-lived at 37 degrees ... | 1999 | 9914499 |
| a built-in arginine finger triggers the self-stimulatory gtpase-activating activity of rho family gtpases. | signal transduction through the rho family gtpases requires regulated cycling of the gtpases between the active gtp-bound state and the inactive gdp-bound state. rho family members containing an arginine residue at position 186 in the c-terminal polybasic region were found to possess a self-stimulatory gtpase-activating protein (gap) activity through homophilic interaction, resulting in significantly enhanced intrinsic gtpase activities. this arginine residue functions effectively as an "arginin ... | 1999 | 9915787 |
| cloning and characterization of prax-1. a new protein that specifically interacts with the peripheral benzodiazepine receptor. | using a cytoplasmic domain of the peripheral benzodiazepine receptor (pbr) as a bait in the yeast two-hybrid system, we have isolated a cdna encoding a new protein that specifically interacts with pbr. we named it prax-1, for peripheral benzodiazepine receptor-associated protein 1. prax-1 is a 1857-amino acid protein, the sequence of which was structurally unrelated to any known proteins. the gene encoding prax-1 is located in the q22-q23 region of the long arm of the human chromosome 17. the pr ... | 1999 | 9915832 |
| cell cycle regulation of the double stranded rna activated protein kinase, pkr. | the interferon (ifn)-induced, double stranded rna (dsrna)-activated serine/threonine kinase, pkr, is a potent negative regulator of cell growth when overexpressed in yeast or mammalian cells. to determine whether endogenous pkr plays a role in cell growth control, we have investigated the regulation of pkr levels and activity during the cell cycle in human glioblastoma t98g cells. the steady-state level of pkr mrna in t98g cells was highest in growth arrested cells, dropped sharply within 3 h of ... | 1999 | 9927188 |
| fog-2: a novel gata-family cofactor related to multitype zinc-finger proteins friend of gata-1 and u-shaped. | gata factors are transcriptional regulatory proteins that play critical roles in the differentiation of multiple cell types in both vertebrates and invertebrates. recent evidence suggests that the biological activities of both mammalian and drosophila gata factors are controlled in part by physical interaction with multitype zinc-finger proteins, friend of gata-1 (fog) and u-shaped (ush), respectively. here we describe a new fog-related polypeptide, designated fog-2, that is likely to participat ... | 1999 | 9927674 |
| dna cleavage and degradation by the sbccd protein complex from escherichia coli. | the sbccd protein is a member of a group of nucleases found in bacteriophage t4 and t5, eubacteria, archaebacteria, yeast, drosophila, mouse and man. evidence from electron microscopy has revealed a distinctive structure consisting of two globular domains linked by a long region of coiled coil, similar to that predicted for the members of the smc family. that a nuclease should have such an unusual structure suggests that its mode of action may be complex. here we show that the protein degrades d ... | 1999 | 9927737 |
| direct interaction of microtubule- and actin-based transport motors. | the microtubule network is thought to be used for long-range transport of cellular components in animal cells whereas the actin network is proposed to be used for short-range transport, although the mechanism(s) by which this transport is coordinated is poorly understood. for example, in sea urchins long-range ca2+-regulated transport of exocytotic vesicles requires a microtubule-based motor, whereas an actin-based motor is used for short-range transport. in neurons, microtubule-based kinesin mo ... | 1999 | 9930703 |
| a transforming mutation enhances the activity of the c-kit soluble tyrosine kinase domain. | an activating mutation (dy814) located in the catalytic domain of the c-kit receptor has been found in mastocytomas from human, mouse and rat. we evaluated the enzymic properties of purified wild-type (wt) and dy814 tyrosine kinase domains expressed in pichia pastoris. a linker encoding the flag epitope was fused to c-kit cdna species, enabling affinity purification of the proteins with anti-flag antibodies. yeast lysates expressing dy814 contained multiple tyrosine-phosphorylated proteins, wher ... | 1999 | 9931308 |
| physical delineation of a 700-kb region overlapping the looptail mutation on mouse chromosome 1. | the mouse looptail (lp) mutation is an established model for neural tube defects with homozygous lp embryos showing an open neural tube from the caudal midbrain to the tip of the tail. heterozygous lp mice are characterized by a "looped-tail" and wobbly head movements. the lp gene has been mapped to a 0.6-cm interval on mouse chromosome 1 delineated by two clusters of markers, fcer1gamma/usf1/d1mit113/d1wsu1 on the proximal side and fcer1alpha/spna1/d1mit149 distally. in the present study, we ha ... | 1999 | 9933565 |
| human and mouse homologs of the schizosaccharomyces pombe rad17+ cell cycle checkpoint control gene. | the schizosaccharomyces pombe rad17+ cell cycle checkpoint control gene is required for s-phase and g2/m arrest in response to both dna damage and incomplete dna replication. we isolated and characterized the putative human (rad17sp) and mouse (mrad17sp) homologs of the s. pombe rad17 (rad17sp) protein. the human rad17sp open reading frame (orf) encodes a protein of 681 amino acids; the mrad17sp orf codes for a protein of 688 amino acids. the mrad17sp messenger is highly expressed in the testis ... | 1999 | 9933569 |
| mammalian homologue of the caenorhabditis elegans unc-76 protein involved in axonal outgrowth is a protein kinase c zeta-interacting protein. | by the yeast two-hybrid screening of a rat brain cdna library with the regulatory domain of protein kinase c zeta (pkczeta) as a bait, we have cloned a gene coding for a novel pkczeta-interacting protein homologous to the caenorhabditis elegans unc-76 protein involved in axonal outgrowth and fasciculation. the protein designated fez1 (fasciculation and elongation protein zeta-1) consisting of 393 amino acid residues shows a high asp/glu content and contains several regions predicted to form amph ... | 1999 | 9971736 |
| the beta-glucan-binding lectin site of mouse cr3 (cd11b/cd18) and its function in generating a primed state of the receptor that mediates cytotoxic activation in response to ic3b-opsonized target cells. | mouse leukocyte cr3 (mac-1, alphambeta2 integrin) was shown to function as a receptor for beta-glucans in the same way as human cr3. soluble zymosan polysaccharide (szp) or pure beta-glucans labeled with fitc or 125i bound in a saturable and reversible manner to neutrophils, macrophages, and nk cells. this lectin activity was blocked by anti-cd11b mab m1/70 or 5c6 and did not occur with leukocytes from cr3-/- (cd11b-deficient) mice. szp preparations containing primarily mannose or glucose bound ... | 1999 | 9973505 |
| the histone acetylase pcaf is a phorbol-ester-inducible coactivator of the irf family that confers enhanced interferon responsiveness. | transcription factors of the interferon regulatory factor (irf) family bind to the type i interferon (ifn)-responsive element (isre) and activate transcription from ifn-inducible genes. to identify cofactors that associate with irf proteins, dna affinity binding assays were performed with nuclear extracts prepared from tissue culture cells. the results demonstrated that the endogenous irfs bound to the isre are complexed with the histone acetylases, pcaf, gcn5, and p300/creb binding protein and ... | 1999 | 10022868 |
| loss of heterozygosity at the dilute-short ear (myo5a-bmp5) region of the mouse: mitotic recombination or double non-disjunction? | the occurrence of homozygous-viable dilute-short ear (myo5a-bmp5) double mutants in mouse specific locus mutation experiments has generally been assumed to be the result of double non-disjunction such that the mutant inherits two copies of chromosome 9 carrying the recessive alleles from the test-stock. a homozygous viable myo5a-bmp5 double mutant was recovered recently in our laboratory. we were able to genetically analyse both the myo5a-bmp5 region and proximal and distal markers in the origin ... | 1998 | 10036975 |
| identification of a novel domain shared by putative components of the endocytic and cytoskeletal machinery. | we have identified a approximately 140 amino acid domain that is shared by a variety of proteins in budding and fission yeast, nematode, rat, mouse, frog, oat, and man. typically, this domain is located within 20 residues of the n-terminus of the various proteins. the percent identity among the domains in the 12 proteins ranges from 42 to 93%, with 16 absolutely conserved residues: n-x(11-13)-v-x2-a-t-x(34-36)-r-x(7-8)-w-r-x3-k-x12-g-x-e-x15 -l-x11-12-d-x-g-r-x11-d-x7-r. even though these protei ... | 1999 | 10048338 |
| cloning of a gadd34-like gene that interacts with the zinc-finger transcription factor which binds to the p21(waf) promoter. | a histone deacetylase inhibitor has been shown to induce differentiation of many cancer cells and senescence-like state of human fibroblasts. previously, our data suggested that the region responsive to trichostatin a (tsa), a specific inhibitor of histone deacetylase, treatment in the p21(waf1) promoter is located -100 bp upstream from transcription initiation site and contains a gc-box where both sp1 and sp3 are responsible. here we show that another zinc-finger transcription factor, bfcol1, w ... | 1999 | 10066455 |
| the mouse genome contains two expressed intronless retroposed pseudogenes for the sentrin/sumo-1/pic1 conjugating enzyme ubc9. | the ubiquitin conjugating (ubc) e2 enzyme ubc-9 conjugates the ubiquitin-like peptide sentrin/sumo-1/pic1 to target proteins which include the fas antigen. we show that the mouse genome contains four copies of the ubc-9 gene. these include a structural ubc-9 gene consisting of seven exons which encode a protein identical to human ubc-9, and three intronless processed pseudogenes. the open reading frames (orf) of two of the pseudogenes, ubc9-psi1 and ubc9-psi2, correspond to the cdna of ubc-9 and ... | 1998 | 10068040 |
| notch signaling imposes two distinct blocks in the differentiation of c2c12 myoblasts. | notch signal transduction regulates expression of downstream genes through the activation of the dna-binding protein su(h)/cbf1. in drosophila most of notch signaling requires su(h); however, some notch-dependent processes occur in the absence of su(h) suggesting that notch signaling does not always involve activation of this factor. using constitutively active forms of notch lacking cbf1-interacting sequences we identified a notch signaling pathway that inhibits myogenic differentiation of c2c1 ... | 1999 | 10079231 |
| subunits of the eukaryotic cytosolic chaperonin cct do not always behave as components of a uniform hetero-oligomeric particle. | the chaperonin cct is an hetero-oligomeric molecular chaperone complex. studies in yeast suggest each of its eight gene products are required for its major identified functions in producing native tubulins and actins. however, it is unclear whether these eight components always form a single particle, covering all functions, or else can also exist as heterogeneous mixtures and/or free subunits in cells. using mouse p19 embryonal carcinoma cells, which divide rapidly, yet in retinoic acid adopt a ... | 1999 | 10082421 |
| mitogen-activated protein kinases: specific messages from ubiquitous messengers. | | 1999 | 10082509 |
| cbp-independent activation of crem and creb by the lim-only protein act. | transcriptional activation by creb and crem requires phosphorylation of a serine residue within the activation domain (ser 133 in creb; ser 117 in crem) which as a result interacts with the coactivator cbp. the activator crem is highly expressed in male germ cells and is required for post-meiotic gene expression. using a two-hybrid screen, we have isolated a testis-derived complementary dna encoding a protein that we term act (for activator of crem in testis), a lim-only protein which specifical ... | 1999 | 10086359 |
| genetic and physical mapping of the lps locus: identification of the toll-4 receptor as a candidate gene in the critical region. | on the basis of 2093 meioses analyzed in two separate intraspecific backcrosses, the location of the mouse lpsd mutation was circumscribed to a genetic interval 0.9 cm in size. a total of 19 genetic markers that lie in close proximity to the mutation were examined in mapping. most of these were previously unpublished polymorphic microsatellites, identified by fragmentation of yac and bac clones spanning the region of interest. lpsd was found to be inseparable from the microsatellite marker d4mit ... | 1998 | 10087992 |
| preparation of yac end fragments from the whitehead/mit mouse yac library prml vectors. | | 1999 | 10090972 |
| delta 9-fatty acid desaturase from arachidonic acid-producing fungus. unique gene sequence and its heterologous expression in a fungus, aspergillus. | based on the sequence information for delta 9-desaturase genes (from rat, mouse and yeast), which are involved in the desaturation of palmitic acid and stearic acid to palmitoleic acid and oleic acid, respectively, the corresponding cdna and genomic gene were cloned from the fungal strain, mortierella alpina 1s-4, which industrially produces arachidonic acid. there was a cytochrome b5-like domain linked to the carboxyl terminus of this mortierella desaturase, as also seen in the yeast delta 9-de ... | 1999 | 10091601 |
| tlk, a novel evolutionarily conserved murine serine threonine kinase, encodes multiple testis transcripts. | hypothesizing that genes important in meiotic processes in mammals might have evolutionarily conserved counterparts in lower organisms, we used the yeast ime2 meiotic gene (serine threonine kinase) as a probe for screening a mouse testis cdna library. this screening resulted in identification of a novel putative serine threonine kinase. although it did not exhibit significant homology to ime2, it did show significant sequence homology to the tousled kinase in arabidopsis. tousled is associated w ... | 1999 | 10092119 |
| hsp27 multimerization mediated by phosphorylation-sensitive intermolecular interactions at the amino terminus. | distinct biochemical activities have been reported for small and large molecular complexes of heat shock protein 27 (hsp27), respectively. using glycerol gradient ultracentrifugation and chemical cross-linking, we show here that chinese hamster hsp27 is expressed in cells as homotypic multimers ranging from dimers up to 700-kda oligomers. treatments with arsenite, which induces phosphorylation on ser15 and ser90, provoked a major change in the size distribution of the complexes that shifted from ... | 1999 | 10092617 |
| rna surveillance. unforeseen consequences for gene expression, inherited genetic disorders and cancer. | messenger rnas are monitored for errors that arise during gene expression by a mechanism called rna surveillance, with the result that most mrnas that cannot be translated along their full length are rapidly degraded. this ensures that truncated proteins are seldom made, reducing the accumulation of rogue proteins that might be deleterious. the pathway leading to accelerated mrna decay is referred to as nonsense-mediated mrna decay (nmd). the proteins that catalyze steps in nmd in yeast serve tw ... | 1999 | 10098411 |
| excision repair of 8-hydroxyguanine in mammalian cells: the mouse ogg1 protein as a model. | 8-hydroxyguanine (8-oh-gua) is a major mutagenic lesion produced on dna by the oxidative stress induced by either the endogen metabolism or the exposure to external agents. in bacteria and yeast this modified base can be removed by specific dna glycosylases. recently a human gene coding for an 8-oh-gua dna glycosylase/ap lyase has been identified by its homology to the yeast ogg1. this gene is located in human chromosome 3p25, a region commonly rearranged in various cancers, specially in lung tu ... | 1998 | 10098454 |
| acn9 is a novel protein of gluconeogenesis that is located in the mitochondrial intermembrane space. | previous studies have indicated that the acn9 protein is involved in gluconeogenesis. yeast mutants defective in the acn9 gene display phenotypes identical with mutants defective in metabolic enzymes required for carbon assimilation. these phenotypes include the inability to utilize acetate as a carbon and energy source, elevated levels of enzymes of the glyoxylate cycle, gluconeogenesis and acetyl-coa mobilization, and a deficiency in de novo synthesis of glucose from ethanol. the acn9 gene was ... | 1999 | 10103055 |
| the mechanisms of death of an erythroleukemic cell line by p53: involvement of the microtubule and mitochondria. | a murine erythroleukemic cell line (1-2-3) which expresses only the temperature-sensitive mutant p53 gene (ala-to-val substitution at codon 135) was established. these cells showed typical characteristics of apoptosis, when they were cultured at 32 degrees c. in this process, p53 recovered the wild-type p53 function and the expression of the p21 (waf1/cip1/sdi1), cyclin g1 and gadd45 genes was increased. however, no significant changes were detected in the expression of the mdm2, bcl-2, bax, fas ... | 1999 | 10194136 |
| cloning, expression, and chromosomal mapping of a human atpase ii gene, member of the third subfamily of p-type atpases and orthologous to the presumed bovine and murine aminophospholipid translocase. | recently, a p-type atpase was cloned from bovine chromaffin granules (b-atpase ii) and a mouse teratocarcinoma cell line (m-atpase ii) and was shown to be homologous to the saccharomyces cerevisiae drs2 gene, the inactivation of which resulted in defective transport of phosphatidylserine. here, we report the cloning from a human skeletal muscle cdna library of a human atpase ii (h-atpase ii), orthologous to the presumed bovine and mouse aminophospholipid translocase (95.3 and 95.9% amino acid id ... | 1999 | 10198212 |
| differentially expressed genes in c6.9 glioma cells during vitamin d-induced cell death program. | c6.9 rat glioma cells undergo a cell death program when exposed to 1, 25-dihydroxyvitamin d3 (1,25-d3). as a global analytical approach, we have investigated gene expression in c6.9 engaged in this cell death program using differential screening of a rat brain cdna library with probes derived from control and 1,25-d3-treated cells. using this methodology we report the isolation of 61 differentially expressed cdnas. forty-seven cdnas correspond to genes already characterized in rat cells or tissu ... | 1998 | 10200452 |
| molecular cloning and sequencing of cdnas encoding homologues of human ku70 and ku80 autoantigen from xenopus and their expression in various xenopus tissues. | we isolated cdna clones encoding ku70 and ku80 homologues of xenopus laevis from a cdna library prepared from xenopus oocytes. the nucleotide sequences of these ku70 and ku80 homologues have coding sequences of 1833 bp and a 611 aa protein, and 2178 bp and a 726 aa protein, respectively. the amino acid sequences deduced from the open reading frame of the ku70 and ku80 cdna clones were highly homologous to those from ku genes previously isolated, such as human (ca. 65% and ca. 62% identity, respe ... | 1999 | 10209269 |
| a mammalian lysosomal membrane protein confers multidrug resistance upon expression in saccharomyces cerevisiae. | mouse transporter protein (mtp) is a highly conserved polytopic membrane protein present in mammalian lysosomes and endosomes. the role of mtp in regulating the in vivo subcellular distribution of numerous structurally distinct small molecules has been examined in this study by its expression in a drug-sensitive strain of the yeast saccharomyces cerevisiae. surprisingly, the expression of mtp in membranes of an intracellular compartment resulted in a cellular resistance or hypersensitivity to a ... | 1999 | 10212276 |
| isolation of ku70-binding proteins (kubs). | dna-dependent protein kinase (dna-pk) plays a critical role in resealing dna double-stand breaks by non-homologous end joining. aside from dna-pk, xrcc4 and dna ligase iv, other proteins which play a role(s) in this repair pathway remain unknown; dna-pk contains a catalytic subunit (dna-pkcs) and a dna binding subunit (ku70 and ku80). we isolated ku70-binding proteins (kub1-kub4) using yeast two-hybrid analyses. sequence analyses revealed kub1 to be apolipoprotein j (apoj), also known as x-ray-i ... | 1999 | 10219089 |
| heterodimeric interactions between chicken ovalbumin upstream promoter-transcription factor family members arp1 and ear2. | members of the chicken ovalbumin upstream promoter-transcription factor (coup-tf) subfamily of orphan nuclear receptors, which minimally includes coup-tfi and arp1, are highly expressed in brain and are generally considered to be constitutive repressors of transcription. we have used a yeast two-hybrid system to isolate proteins expressed in brain that interact with arp1. one of the proteins isolated in this screen was ear2, another orphan receptor that has been suggested to be a member of the c ... | 1999 | 10318855 |
| the mouse major histocompatibility complex: some assembly required. | we have assembled a contig of 81 yeast artificial chromosome clones that spans 8 mb and contains the entire major histocompatibility complex (mhc) from mouse strain c57bl/6 (h2b), and we are in the process of assembling an mhc contig of bacterial artificial chromosome (bac) clones from strain 129 (h2bc), which differs from c57bl/6 in the h2-q and h2-t regions. the current bac contig extends from tapasin to d17leh89 with gaps in the class ii, h2-q, and distal h2-m regions. only four bac clones we ... | 1999 | 10319263 |
| identification of chip, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. | the chaperone function of the mammalian 70-kda heat shock proteins hsc70 and hsp70 is modulated by physical interactions with four previously identified chaperone cofactors: hsp40, bag-1, the hsc70-interacting protein hip, and the hsc70-hsp90-organizing protein hop. hip and hop interact with hsc70 via a tetratricopeptide repeat domain. in a search for additional tetratricopeptide repeat-containing proteins, we have identified a novel 35-kda cytoplasmic protein, carboxyl terminus of hsc70-interac ... | 1999 | 10330192 |
| a human immunoglobulin lambda locus is similarly well expressed in mice and humans. | transgenic mice carrying a 380-kb region of the human immunoglobulin (ig) lambda light (l) chain locus in germline configuration were created. the introduced translocus on a yeast artificial chromosome (yac) accommodates the most proximal iglambda variable region (v) gene cluster, including 15 vlambda genes that contribute to >60% of lambda l chains in humans, all jlambda-clambda segments, and the 3' enhancer. huiglambdayac mice were bred with animals in which mouse igkappa production was silenc ... | 1999 | 10330440 |
| t-star/etoile: a novel relative of sam68 that interacts with an rna-binding protein implicated in spermatogenesis. | rbm is an rna-binding protein encoded on the y chromosome in mammals and is expressed only in the nuclei of male germ cells. genetic evidence from infertile men implicates it in spermatogenesis, but its function is unknown. of a number of potential partners for rbm identified by a yeast two-hybrid screen with testis cdna, the most frequent isolates encoded a novel rna-binding protein, termed t-star, that is closely related to sam68, an src-associated protein of unknown function. the mouse homolo ... | 1999 | 10332027 |
| signal presequences increase mitochondrial permeability and open the multiple conductance channel. | we have reported that the signal presequence of cytochrome oxidase subunit iv from neurospora crassa increases the permeability of isolated rat liver mitochondria [p. m. sokolove and k. w. kinnally (1996) arch. biochem. biophys. 336, 69] and regulates the behavior of the mutiple conductance channel (mcc) of yeast inner mitochondrial membrane [t. a. lohret and k. w. kinnally (1995) j. biol. chem. 270, 15950]. here we examine in greater detail the action of a number of mitochondrial presequences f ... | 1999 | 10334870 |
| hepatitis c virus core protein binds to a dead box rna helicase. | approximately 4 million americans are infected with the hepatitis c virus (hcv), making it a major cause of chronic liver disease. because of the lack of an efficient cell culture system, little is known about the interaction between hcv and host cells. we performed a yeast two-hybrid screen of a human liver cell cdna library with hcv core protein as bait and isolated the dead box protein dbx. dbx has significant amino acid sequence identity to mouse pl10, an atp-dependent rna helicase. the bind ... | 1999 | 10336476 |
| efficiency of importin alpha/beta-mediated nuclear localization sequence recognition and nuclear import. differential role of ntf2. | little quantitative, kinetic information is available with respect to the process of nuclear import of conventional nuclear localization sequence (nls)-containing proteins, which initially involves recognition and docking at the nuclear pore by importin alpha/beta. this study compares the binding and nuclear import properties of mouse (m) and yeast (y) importin (imp) subunits with respect to the nlss from the sv40 large tumor antigen (t-ag), and the xenopus laevis phosphoprotein n1n2. m- and y-i ... | 1999 | 10336485 |
| activation of the murine dihydrofolate reductase promoter by e2f1. a requirement for cbp recruitment. | the e2f family of heterodimeric transcription factors plays an important role in the regulation of gene expression at the g1/s phase transition of the mammalian cell cycle. previously, we have demonstrated that cell cycle regulation of murine dihydrofolate reductase (dhfr) expression requires e2f-mediated activation of the dhfr promoter in s phase. to investigate the mechanism by which e2f activates an authentic e2f-regulated promoter, we precisely replaced the e2f binding site in the dhfr promo ... | 1999 | 10336493 |
| characterization of the mammalian septin h5: distinct patterns of cytoskeletal and membrane association from other septin proteins. | the mechanisms controlling cytokinesis during yeast budding and animal cell fission appear quite different, yet both require members of the septin protein family. mammalian homologs of this novel family of gtpases have been identified but little is known about their properties or functions. using an antibody specific for the mammalian septin h5, we show that this protein is expressed at distinct levels in a variety of tissues. tissue expression levels in different tissues did not coincide with t ... | 1999 | 10340703 |
| analysis of intrachromosomal homologous recombination in mammalian cell, using tandem repeat sequences. | in all the organisms, homologous recombination (hr) is involved in fundamental processes such as genome diversification and dna repair. several strategies can be devised to measure homologous recombination in mammalian cells. we present here the interest of using intrachromosomal tandem repeat sequences to measure hr in mammalian cells and we discuss the differences with the ectopic plasmids recombination. the present review focuses on the molecular mechanisms of hr between tandem repeats in mam ... | 1999 | 10343649 |
| technology development at the interface of proteome research and genomics: mapping nonpolymorphic proteins on the physical map of mouse chromosomes. | data obtained from protein spots by peptide mass fingerprinting are used to identify the corresponding genes in sequence databases. the relevant cdnas are obtained as clones from the integrated molecular analysis of genome expression (i.m.a.g.e.) consortium. mapping of i.m.a.g.e. clones is performed in two steps: first, cdna clones are hybridized against a 10-hit genomic mouse bacterial artificial chromosome (bac) library. second, interspersed repetitive sequence polymerase chain reaction (irs-p ... | 1999 | 10344281 |
| stress signaling from the lumen of the endoplasmic reticulum: coordination of gene transcriptional and translational controls. | | 1999 | 10346810 |
| yac transgenic analysis reveals wilms' tumour 1 gene activity in the proliferating coelomic epithelium, developing diaphragm and limb. | wilms' tumour 1 gene (wt1) is required for the correct development of the urogenital system. to examine its regulation and expression, we created several transgenic mouse lines containing a beta-galactosidase reporter driven by the human wt1 promoter. a 5 kb promoter weakly recapitulated a subset of the endogenous wt1 expression pattern. in contrast, 470 and 280 kb yac transgenes reproduced the correct pattern with high activity and highlighted new expression sites. wt1 is expressed in the septu ... | 1998 | 10349631 |
| hpop4: a new protein subunit of the human rnase mrp and rnase p ribonucleoprotein complexes. | rnase mrp is a ribonucleoprotein particle involved in the processing of pre-rrna. the rnase mrp particle is structurally highly related to the rnase p particle, which is involved in pre-trna processing. their rna components fold into a similar secondary structure and they share several protein subunits. we have identified and characterised human and mouse cdnas that encode proteins homologous to ypop4p, a protein subunit of both the yeast rnase mrp and rnase p complexes. the human pop4 cdna enco ... | 1999 | 10352175 |
| a thioester analogue of an amino acetylenic aldehyde is a suicide inhibitor of aldehyde dehydrogenase and an inducer of apoptosis in mouse lymphoid cells overexpressing the bcl2 gene. | | 1999 | 10352674 |
| identification of the cell cycle regulator vcp (p97/cdc48) as a substrate of the band 4.1-related protein-tyrosine phosphatase ptph1. | the human band 4.1-related protein-tyrosine phosphatase ptph1 was introduced into nih3t3 cells under the control of a tetracycline-repressible promoter. ectopic expression of wild type ptph1 dramatically inhibited cell growth, whereas a catalytically impaired mutant showed no effect. to identify the direct target of ptph1 in the cell, we generated a substrate-trapping mutant, in which an invariant aspartate residue was changed to alanine (d811a in ptph1). the ptph1-d811a mutant trapped primarily ... | 1999 | 10364224 |
| identification of the stef gene that encodes a novel guanine nucleotide exchange factor specific for rac1. | the rho family gtpases are involved in a variety of cellular events by changing the organization of actin cytoskeletal networks in response to extracellular signals. however, it is not clearly known how their activities are spatially and temporally regulated. here we report the identification of a novel guanine nucleotide exchange factor for rac1, stef, which is related in overall amino acid sequence and modular structure to mouse tiam1 and drosophila sif proteins. stef protein contains two plec ... | 1999 | 10364228 |
| dominant negative murine serum response factor: alternative splicing within the activation domain inhibits transactivation of serum response factor binding targets. | primary transcripts encoding the mads box superfamily of proteins, such as mef2 in animals and zema in plants, are alternatively spliced, producing several isoformic species. we show here that murine serum response factor (srf) primary rna transcripts are alternatively spliced at the fifth exon, deleting approximately one-third of the c-terminal activation domain. among the different muscle types examined, visceral smooth muscles have a very low ratio of srfdelta5 to srf. increased levels of srf ... | 1999 | 10373507 |
| the disabled 1 phosphotyrosine-binding domain binds to the internalization signals of transmembrane glycoproteins and to phospholipids. | disabled gene products are important for nervous system development in drosophila and mammals. in mice, the dab1 protein is thought to function downstream of the extracellular protein reln during neuronal positioning. the structures of dab proteins suggest that they mediate protein-protein or protein-membrane docking functions. here we show that the amino-terminal phosphotyrosine-binding (ptb) domain of dab1 binds to the transmembrane glycoproteins of the amyloid precursor protein (app) and low- ... | 1999 | 10373567 |
| cloning, mapping and expression of ubl3, a novel ubiquitin-like gene. | a novel drosophila melanogaster gene ubl3 was characterized and shown to be highly conserved in man and caenorhabditis elegans (c. elegans). the human and mouse homologues were cloned and sequenced. ubl3 is a ubiquitin-like protein of unknown function with no conserved homologues in yeast. mapping of the human and mouse ubl3 genes places them within a region of shared gene order between human and mouse chromosomes on human chromosome 13q12-13 and telomeric mouse chromosome 5 (mmu5). | 1999 | 10375635 |
| deletion mapping at 12p12-13 in metastatic prostate cancer. | the identification of homozygous deletions in malignant tissue is a powerful tool for the localization of tumor suppressor genes. representational difference analysis (rda) uses selective hybridization and the polymerase chain reaction (pcr) to isolate regions of chromosomal loss and has facilitated the identification of tumor suppressor genes, such as brca2 and pten. we have recently identified a 1-5-cm homozygous deletion on 12p12-13 in a prostate cancer xenograft and found that 47% of patient ... | 1999 | 10379873 |
| mbp1: a novel mutant p53-specific protein partner with oncogenic properties. | using a yeast two-hybrid screening strategy with a common tumour-derived p53 mutant as bait, we identified several mutant p53-interacting partners including the known proteins wild-type (wt) p53, hubc9 and gbp/pias1. in addition, a novel protein partner was identified which we have termed mbp1, for mutant p53-binding protein 1. mbp1 is a new member of the emerging fibulin gene family, which currently comprises fibulin-1, fibulin-2 and s1-5. expression of mbp1 mrna is differentially regulated bot ... | 1999 | 10380882 |
| metaxin 1 interacts with metaxin 2, a novel related protein associated with the mammalian mitochondrial outer membrane. | a recently described protein, metaxin 1, serves as a component of a preprotein import complex in the outer membrane of the mammalian mitochondrion. a yeast two-hybrid screen with metaxin 1 as bait has now identified a novel protein, which we have termed metaxin 2, as a metaxin 1-binding protein. metaxin 2 shares 29% identity with metaxin 1 at the amino acid level, but metaxin 2, unlike metaxin 1, lacks a c-terminal mitochondrial outer membrane signal-anchor domain. two c. elegans hypothetical pr ... | 1999 | 10381257 |
| genetic and physical maps of the mouse rd3 locus; exclusion of the ortholog of ush2a. | the rd3 retinal degeneration gene was previously mapped 10 +/- 2.5 cm distal to akp1 on mouse chromosome (chr) 1 (chang et al., 1993), a region that may be homologous to the locus of the human ush2a gene, which carries mutations responsible for usher iia retinal degeneration/hearing loss syndrome. an intercross from an rb(11, 13)4bnr(rd3/rd3) x c57bl/6j mating was set up, 428 f2 meioses were analyzed, and the rd3 gene was placed between the markers d1mit292/d1mit209 and d1mit510, a distance of 1 ... | 1999 | 10384036 |
| highly divergent amino termini of the homologous human alr and yeast scerv1 gene products define species specific differences in cellular localization. | the yeast scerv1 gene product is involved in the biogenesis of mitochondria and is indispensable for viability and regulation of the cell cycle. recently the general importance of this gene for the eukaryotic cell was shown by the identification of a structural and functional human homologue. the homologous mammalian alr (augmenter of liver regeneration) genes from man, mouse and rat are involved in the phenomenon of liver regeneration. a low expression rate of the genes is found in all investig ... | 1999 | 10384986 |
| the zinc finger protein a20 inhibits tnf-induced nf-kappab-dependent gene expression by interfering with an rip- or traf2-mediated transactivation signal and directly binds to a novel nf-kappab-inhibiting protein abin. | the zinc finger protein a20 is a tumor necrosis factor (tnf)- and interleukin 1 (il-1)-inducible protein that negatively regulates nuclear factor-kappa b (nf-kappab)-dependent gene expression. however, the molecular mechanism by which a20 exerts this effect is still unclear. we show that a20 does not inhibit tnf- induced nuclear translocation and dna binding of nf-kappab, although it completely prevents the tnf- induced activation of an nf-kappab-dependent reporter gene, as well as tnf-induced i ... | 1999 | 10385526 |
| cloning, characterisation, and functional expression of the mus musculus skd1 gene in yeast demonstrates that the mouse skd1 and the yeast vps4 genes are orthologues and involved in intracellular protein trafficking. | the mouse skd1 protein displays a high degree of sequence identity (62%) to the yeast vps4 protein, which is involved in the transport of proteins out of a prevacuolar/endosomal compartment. we isolated the mouse skd1 locus and found that the skd1 gene is split into 11 exons covering a region of 29kb of the genome. interestingly, the exon/intron structure reflects to a certain degree the proposed domain structure of the protein, since the 5' located coiled-coil region and the aaa domain are flan ... | 1999 | 10393249 |
| cloning and characterization of ash2l and ash2l, human and mouse homologs of the drosophila ash2 gene. | drosophila ash2 belongs to the trithorax (trx) gene family. the ash2 product positively regulates expression of homeotic selector genes, and is implicated in early development and formation of various disc patterns in the fruit fly. through large-scale sequencing of human genomic dna coupled with in silico gene trapping, we identified a gene (ash2l) on chromosome 8p11.2 whose predicted product was highly homologous to ash2, characterized it, and identified its mouse counterpart. the human ash2 c ... | 1999 | 10393421 |
| recruitment of cyclin t1/p-tefb to an hiv type 1 long terminal repeat promoter proximal rna target is both necessary and sufficient for full activation of transcription. | transcriptional activation of the hiv type 1 (hiv-1) long terminal repeat (ltr) promoter element by the viral tat protein is an essential step in the hiv-1 life cycle. tat function is mediated by the tar rna target element encoded within the ltr and is known to require the recruitment of a complex consisting of tat and the cyclin t1 (cyct1) component of positive transcription elongation factor b (p-tefb) to tar. here, we demonstrate that both tar and tat become entirely dispensable for activatio ... | 1999 | 10393900 |