| bioactive prenylated xanthones from the young fruits and flowers of garcinia cowa. | five new xanthones, garciniacowones a-e (1-5), together with 14 known xanthones, 6-19, were isolated from the young fruits and fresh flowers of garcinia cowa. the structures of 1-5 were elucidated by analysis of their 1d and 2d nmr spectra and mass spectrometric data. the compounds 1-19 were tested in vitro for their antimicrobial activity and for their ability to inhibit α-glucosidase. compounds 16 and 17 showed the most potent α-glucosidase inhibitory activity, with ic50 values of 7.8 ± 0.5 an ... | 2015 | 25651042 |
| evaluation of antioxidant, total phenol and flavonoid content and antimicrobial activities of artocarpus altilis (breadfruit) of underutilized tropical fruit extracts. | artocarpus altilis (breadfruit) pulp, peel and whole fruit were extracted with various solvents such as hexane, dichloromethane (dcm) and methanol. the antioxidant activity of these extracts were examined using the stable 2,2-diphenyl-1-picrylhydrazyl (dpph) radical scavenging test. ic50 was 55 ± 5.89 μg/ml for the pulp part of methanol extract. in the β-carotene bleaching assay, the antioxidant activity was 90.02 ± 1.51 % for the positive control (trolox) and 88.34 ± 1.31 % for the pulp part of ... | 2015 | 25649443 |
| microbial diversity of mangrove sediment in shenzhen bay and gene cloning, characterization of an isolated phytase-producing strain of spc09 b. cereus. | phytases hydrolyze phytate to release inorganic phosphate, which decreases the requirement for phosphorus in fertilizers for crops and thus reduces environmental pollutants. this study analyzed microbial communities in rhizosphere sediment, collected in september 2012 from shenzhen bay, guangdong, china, using high-throughput pyrosequencing; the results showed that the dominant taxonomic phyla were chloroflexi, firmicutes, and proteobacteria, and the proportion of the beneficial bacteria, bacill ... | 2015 | 25646962 |
| characterisation of antimicrobial extracts from dandelion root (taraxacum officinale) using lc-spe-nmr. | plant extracts have traditionally been used as sources of natural antimicrobial compounds, although in many cases, the compounds responsible for their antimicrobial efficacy have not been identified. in this study, crude and dialysed extracts from dandelion root (taraxacum officinale) were evaluated for their antimicrobial properties against gram positive and gram negative bacterial strains. the methanol hydrophobic crude extract (dre3) demonstrated the strongest inhibition of microbial growth a ... | 2015 | 25644491 |
| one-day pulsed-field gel electrophoresis protocol for rapid determination of emetic bacillus cereus isolates. | bacillus cereus, the gram-positive and spore-forming ubiquitous bacterium, may cause emesis as the result of food intoxication with cereulide, a heat-stable emetic toxin. rapid determination of cereulide-positive b. cereus isolates is of highest importance due to consequences of this intoxication for human health and life. here we present a 1-day pulsed-field gel electrophoresis for emetic b. cereus isolates, which allows rapid and efficient determination of their genomic relatedness and helps d ... | 2015 | 25639850 |
| protein structure. structure and activity of tryptophan-rich tspo proteins. | translocator proteins (tspos) bind steroids and porphyrins, and they are implicated in many human diseases, for which they serve as biomarkers and therapeutic targets. tspos have tryptophan-rich sequences that are highly conserved from bacteria to mammals. here we report crystal structures for bacillus cereus tspo (bctspo) down to 1.7 å resolution, including a complex with the benzodiazepine-like inhibitor pk11195. we also describe bctspo-mediated protoporphyrin ix (ppix) reactions, including ca ... | 2015 | 25635100 |
| phenolic acids profile, antioxidant and antibacterial activity of chamomile, common yarrow and immortelle (asteraceae). | chamomile, common yarrow and immortelle ethanolic extracts were chemically analysed with respect to phenolics. twelve phenolic acids were separated and identified by hplc-dad and the presence of rosmarinic acid was additionally confirmed by lc-ms. five methods were applied for the evaluation of extracts' antioxidant properties (frap, dpph, abts, chelating activity, briggs-rauscher reaction), while the antibacterial activity was tested against some of the major food-borne pathogens (campylobacter ... | 2014 | 25632475 |
| quantitative and qualitative evaluation of bio-aerosols in surgery rooms and emergency department of an educational hospital. | bio-aerosols are a potential hazard in hospitals and are mostly produced by hospital staff, patients and visitors. bio-aerosols are solid or liquid particles pending in the air and they consist of aerosols accompanying micro-organisms or organic compounds of micro-organisms such as endotoxin, metabolite, toxin and other parts of organism. those are a potential hazard in hospitals and are mostly produced by hospital staff, patients and visitors. | 2014 | 25632321 |
| an outbreak of gastroenteritis linked to a buffet lunch served at a canberra restaurant. | in 2013, an outbreak of gastrointestinal illness occurred following a buffet lunch at a restaurant in canberra. an investigation was conducted to identify the cause of illness and to implement appropriate public health measures to prevent further disease. we conducted a retrospective cohort study via telephone interviews, using a structured questionnaire developed from the restaurant buffet menu. a case was defined as someone who ate the buffet lunch at the restaurant on the implicated date and ... | 2014 | 25631587 |
| food-grade antimicrobials potentiate the antibacterial activity of 1,2-hexanediol. | preservative agents determining the shelf life of cosmetic products must have effective antimicrobial activity while meeting safety requirements for topical use. in this study, we determined the antimicrobial activity of 1,2-hexanediol against several gram-positive and gram-negative bacteria. antimicrobial susceptibility tests have shown that 1,2-hexanediol exhibits broad-spectrum activity against gram-positive and gram-negative bacteria with mics of 0·5-2% (v/v). the bactericidal concentration ... | 2015 | 25631558 |
| analysis of bacillus cereus toxicity using pcr, elisa and a lateral flow device. | the aim of this study was to evaluate the performance of immunodetection methods and pcr analysis of enterotoxigenic bacillus cereus strains. | 2015 | 25627167 |
| introducing urtica dioica, a native plant of khuzestan, as an antibacterial medicinal plant. | urtica dioica is a flowering plant with long history of use in folk medicine and as a food source. | 2014 | 25625045 |
| evaluation of haccp system implementation on the quality of mixed fresh-cut salad prepared in a university canteen: a case study. | the increasing awareness that foods can represent vehicles for health risk factors has caused scientists and public authorities to multiply their efforts to reduce these risks to within acceptable limits. nevertheless, some challenging issues still remain unsolved and new ones have recently emerged, such as the increase in outbreaks of foodborne diseases originating from the consumption of meals at catering facilities. the study described in this article was aimed at evaluating the microbiologic ... | 2015 | 25619040 |
| effects of climate change on the persistence and dispersal of foodborne bacterial pathogens in the outdoor environment: a review. | according to the intergovernmental panel on climate change (ipcc), warming of the climate system is unequivocal. over the coming century, warming trends such as increased duration and frequency of heat waves and hot extremes are expected in some areas, as well as increased intensity of some storm systems. climate-induced trends will impact the persistence and dispersal of foodborne pathogens in myriad ways, especially for environmentally ubiquitous and/or zoonotic microorganisms. animal hosts of ... | 2016 | 25612827 |
| plasmids are vectors for redundant chromosomal genes in the bacillus cereus group. | prokaryotic plasmids have played significant roles in the evolution of bacterial genomes and have a great impact on the metabolic functions of the host cell. many bacterial strains contain multiple plasmids, but the relationships between bacterial plasmids and chromosomes are unclear. we focused on plasmids from the bacillus cereus group because most strains contain several plasmids. | 2015 | 25608745 |
| squid pen chitin chitooligomers as food colorants absorbers. | one of the most promising applications of chitosanase is the conversion of chitinous biowaste into bioactive chitooligomers (cos). tku033 chitosanase was induced from squid pen powder (spp)-containing bacillus cereus tku033 medium and purified by ammonium sulfate precipitation and column chromatography. the enzyme was relatively more thermostable in the presence of the substrate and had an activity of 93% at 50 °c in a ph 5 buffer solution for 60 min. furthermore, the enzyme used for the cos pre ... | 2015 | 25608726 |
| evaluation of bacillus cereus and bacillus pumilus metabolites for anthelmintic activity. | to assess the anthelmintic acivity of bacillus cereus and bacillus pumilus metabolites. | 2017 | 25598639 |
| characteristics of enterotoxin distribution, hemolysis, lecithinase, and starch hydrolysis of bacillus cereus isolated from infant formulas and ready-to-eat foods. | bacillus cereus is a ubiquitous environmental microbe implicated as a main cause of food poisoning with various symptoms, depending on the strain type and the isolation source. in this study, the potential virulence factors and biochemical properties of b. cereus isolated from infant formulas and ready-to-eat (rte) foods were analyzed and compared. a total of 347 b. cereus strains were isolated and identified from 687 infant food formulas and rte food samples. all the isolates had one or more en ... | 2015 | 25597976 |
| bacteriophage pbc1 and its endolysin as an antimicrobial agent against bacillus cereus. | bacillus cereus is an opportunistic human pathogen responsible for food poisoning and other, nongastrointestinal infections. due to the emergence of multidrug-resistant b. cereus strains, the demand for alternative therapeutic options is increasing. to address these problems, we isolated and characterized a siphoviridae virulent phage, pbc1, and its lytic enzymes. pbc1 showed a very narrow host range, infecting only 1 of 22 b. cereus strains. phylogenetic analysis based on the major capsid prote ... | 2015 | 25595773 |
| finished genome sequence of bacillus cereus strain 03bb87, a clinical isolate with b. anthracis virulence genes. | bacillus cereus strain 03bb87, a blood culture isolate, originated in a 56-year-old male muller operator with a fatal case of pneumonia in 2003. here we present the finished genome sequence of that pathogen, including a 5.46-mb chromosome and two plasmids (209 and 52 kb, respectively). | 2015 | 25593267 |
| antimicrobial potential of cauliflower, broccoli, and okara byproducts against foodborne bacteria. | the antimicrobial potential of cauliflower, broccoli, and okara byproducts was assessed against gram-positive and gram-negative bacteria. salmonella enterica serovar typhimurium, escherichia coli o157:h7, bacillus cereus, and listeria monocytogenes serovar 4b growth behavior was assessed under exposure to 5% vegetable byproducts added to the reference medium, buffered peptone water (0.1% [wt/vol]), at 37°c. although the byproducts were not effective against l. monocytogenes, they were bactericid ... | 2015 | 25587927 |
| slow leakage of ca-dipicolinic acid from individual bacillus spores during initiation of spore germination. | when exposed to nutrient or nonnutrient germinants, individual bacillus spores can return to life through germination followed by outgrowth. laser tweezers, raman spectroscopy, and either differential interference contrast or phase-contrast microscopy were used to analyze the slow dipicolinic acid (dpa) leakage (normally ∼20% of spore dpa) from individual spores that takes place prior to the lag time, tlag, when spores begin rapid release of remaining dpa. major conclusions from this work with b ... | 2015 | 25583976 |
| propidium monoazide combined with real-time pcr for selective detection of viable staphylococcus aureus in milk powder and meat products. | staphylococcus aureus is a spherical, gram-positive, pathogenic bacterium commonly associated with bovine mastitis and clinical infections. it is also recognized as a pathogen that causes outbreaks of food poisoning. the objective of this study was to develop and evaluate a rapid and reliable technique that combines propidium monoazide (pma) staining with real-time quantitative (q)pcr to detect and quantify viable cells of staph. aureus in milk powder and meat products. the inclusivity and exclu ... | 2015 | 25582587 |
| morphological and mechanical imaging of bacillus cereus spore formation at the nanoscale. | bacteria from the genus bacillus are able to transform into metabolically dormant states called (endo) spores in response to nutrient deprivation and other harsh conditions. these morphologically distinct spores are fascinating constructs, amongst the most durable cells in nature, and have attracted attention owing to their relevance in food-related illnesses and bioterrorism. observing the course of bacterial spore formation (sporulation) spatially, temporally and mechanically, from the vegetat ... | 2015 | 25581787 |
| characterization of a nanoscale s-layer protein based template for biomolecular patterning. | well organized template for biomolecular conjugation is the foundation for biosensing. most of the current devices are fabricated using lithographic patterning processes and self-assembly monolayer (sam) methods. however, the research toward developing a sub-10 nm patterned, self-regenerated template on various types of substrates is limited, mainly due to the limited functional groups of the building material. bacterial surface layer proteins (s-layer proteins) can self-assemble into ordered la ... | 2014 | 25570568 |
| hemorrhagic bullous lesions due to bacillus cereus in a cirrhotic patient. | | 2014 | 25566578 |
| antimicrobial activities of three medicinal plants and investigation of flavonoids of tripleurospermum disciforme. | rosa damascena, tripleurospermum disciforme and securigera securidaca were used as disinfectant agents and for treatment of some disease in folk medicine of iran. the antimicrobial effects of different fractions of seeds extract of s. securidaca, petals extract of r. damascena and aerial parts extract of t. disciforme were examined against some gram positive, gram negative and fungi by cup plate diffusion method. the petroleum ether and chloroform fractions of s. securidaca showed antibacterial ... | 2015 | 25561928 |
| improved efficiency and stability of secnidazole - an ideal delivery system. | secnidazole (α,2-dimethyl-5-nitro-1h-imidazole-1-ethanol) is a highly effective drug against a variety of g(+)/g(-) bacteria but with significant side effects because it is being used in very high concentration. in this study, gold nanoparticles (gnps) were selected as a vehicle to deliver secnidazole drug at the specific site with more accuracy which made the drug highly effective at substantially low concentrations. the as-synthesized gnps were capped with human serum albumin (hsa) and subsequ ... | 2015 | 25561882 |
| carvacrol suppresses high pressure high temperature inactivation of bacillus cereus spores. | the inactivation of bacterial spores generally proceeds faster and at lower temperatures when heat treatments are conducted under high pressure, and high pressure high temperature (hpht) processing is, therefore, receiving an increased interest from food processors. however, the mechanisms of spore inactivation by hpht treatment are poorly understood, particularly at moderately elevated temperature. in the current work, we studied inactivation of the spores of bacillus cereus f4430/73 by hpht tr ... | 2015 | 25560915 |
| stem bark extract and fraction of persea americana (mill.) exhibits bactericidal activities against strains of bacillus cereus associated with food poisoning. | the study investigates the in vitro antibacterial potentials of stem bark extracts of persea americana on strains of bacillus cereus implicated in food poisoning. the crude stem bark extracts and butanolic fraction at a concentration of 25 mg/ml and 10 mg/ml, respectively, exhibited antibacterial activities against test isolates. the zones of inhibition exhibited by the crude extract and the fraction ranged between 10 mm and 26 mm, while the minimum inhibitory concentration values ranged between ... | 2014 | 25558854 |
| toxigenic potential and heat survival of spore-forming bacteria isolated from bread and ingredients. | fifty-four spore-forming bacterial strains isolated from bread ingredients and bread, mainly belonging to the genus bacillus (including bacillus cereus), together with 11 reference strains were investigated to evaluate their cytotoxic potential and heat survival in order to ascertain if they could represent a risk for consumer health. therefore, we performed a screening test of cytotoxic activity on ht-29 cells using bacterial culture filtrates after growing bacterial cells in brain heart infusi ... | 2015 | 25555227 |
| enhancement of ε-poly-l-lysine (ε-pl) production by a novel producer bacillus cereus using metabolic precursors and glucose feeding. | epsilon poly-l-lysine (ε-pl) is a homo-biopolymer with approximately 25-30 l-lysine residues. it is a promising natural biopolymer widely used in food and pharmaceutical industry. the present work reports enhanced production of ε-pl with a novel producer bacillus cereus using amino acids and tca cycle intermediates in the fermentation medium. among the various amino acids and tca cycle intermediates tested 2 mm l-aspartic acid and 5 mm citric acid gave ε-pl yield of 145.5 and 230 mg/l, respectiv ... | 2015 | 28324535 |
| correction: genomic comparison of 93 bacillus phages reveals 12 clusters, 14 singletons and remarkable diversity. | the bacillus genus of firmicutes bacteria is ubiquitous in nature and includes one of the best characterized model organisms, b. subtilis, as well as medically significant human pathogens, the most notorious being b. anthracis and b. cereus. as the most abundant living entities on the planet, bacteriophages are known to heavily influence the ecology and evolution of their hosts, including providing virulence factors. thus, the identification and analysis of bacillus phages is critical to underst ... | 2014 | 25547158 |
| spirotetronate antibiotics with anti-clostridium activity from actinomadura sp. 2eps. | the rare actinomycetes strain 2eps was isolated from soil and analysis of cultural, morphological characteristics, diaminopimelic acid content of its cell wall, and 16s rrna gene sequence indicates that 2eps belongs to genus actinomadura. in addition, neighbor-joining phylogenetic tree also confirmed the relationships of this strain to other members of actinomadura. a butanol extract with antibacterial activity was purified by reversed-phase chromatography to obtain three bioactive compounds, de ... | 2015 | 25543910 |
| biosynthesis of hematite nanoparticles and its cytotoxic effect on hepg2 cancer cells. | iron oxide nanoparticles were gaining significant importance in a variety of applications due to its paramagnetic properties and biocompatibility. various chemical methods were employed for hematite nanoparticle synthesis which require special equipment or a complex production process. in this study, protein capped crystalline hexagonal hematite (α-fe2o3) nanoparticles were synthesized by green approach using culture supernatant of a newly isolated bacterium, bacillus cereus svk1 at ambient cond ... | 2015 | 25542166 |
| activity against microorganisms affecting cellulosic objects of the volatile constituents of leonotis nepetaefolia from nicaragua. | the chemical composition of the essential oil from aerial parts of l. nepetaefolia (l.) r. br. collected in nicaragua was evaluated by gc and gc-ms. the main components were (z)-phytol (22.8%), caryophyllene oxide (18.9%) and hexahydrofarnesylacetone (9.0%). the antibacterial activity against several gram + and gram - bacteria, including bacillus cereus and b. subtilis, both infesting historical cellulosic material, was also determined. b. cereus, b. subtilis and staphylococcus epidermis were th ... | 2014 | 25532300 |
| characterization of a novel thermostable n-acylhomoserine lactonase from the thermophilic bacterium thermaerobacter marianensis. | thermaerobacter marianensis is an extremely thermophilic bacterium, which was isolated from the mariana trench, with an optimal growth temperature of approximately 75 °c. n-acylhomoserine lactone (ahl) is a quorum-sensing signal molecule used by many gram-negative bacteria. here, we report the identification of an ahl-degrading gene homolog (designated aiit) in the genome of t. marianensis jcm 10246. aiit has 59.7%, 21.2%, and 11.2% identity to ahls from solibacillus silvestris, aiia from bacill ... | 2015 | 25529553 |
| characteristics and phylogeny of bacillus cereus strains isolated from maari, a traditional west african food condiment. | maari is a spontaneously fermented food condiment made from baobab tree seeds in west african countries. this type of product is considered to be safe, being consumed by millions of people on a daily basis. however, due to the spontaneous nature of the fermentation the human pathogen bacillus cereus occasionally occurs in maari. this study characterizes succession patterns and pathogenic potential of b. cereus isolated from the raw materials (ash, water from a drilled well (dw) and potash), seed ... | 2015 | 25528535 |
| draft genome sequence of new bacillus cereus strain tsu1. | this paper reports the draft genome sequence of new bacillus cereus strain tsu1, isolated on an agar-cellulose plate. the draft genome sequence is 5.81 mb, revealing 5,673 coding sequences. it contains genes for cellulose-degradation and biosynthesis pathways of polyhydroxybutyrate (phb) and 8 rrna genes (5s, 16s, and 23s). | 2014 | 25523768 |
| evaluating the efficiency of humic acid to remove micro-organisms from denture base material. | to evaluate the efficiency of humic acid substances on removing micro-organisms from denture base materials. | 2016 | 25523597 |
| quantitative analysis of cereulide toxin from bacillus cereus in rice and pasta using synthetic cereulide standard and 13c6-cereulide standard - a short validation study. | a single laboratory validation study of a rapid and sensitive quantitative method for the analysis of cereulide toxin produced by bacillus cereus using ultra high performance liquid chromatography-electrospray-tandem mass spectrometry is presented. the analysis of this cyclic peptide toxin was validated for pasta and rice samples using a newly presented synthetic cereulide peptide standard, together with 13c6-cereulide that previously have not been commercially available. the use of cereulide st ... | 2014 | 25514093 |
| [electron microscopy of the surfaces of bacillary spores]. | the surface structures of the spores of bacillus. cereus, b. thuringiensis, and brevibacillus laterosporus were studied by transmission and scanning electron microscopy. platinum deposition and negative staining with uranyl acetate revealed appendages and exosporium in b. thuringiensis and b. cereus. the exosporium structure was visualized by negative staining and ultrathin sectioning. for staining the exosporium polysaccharide, alcian blue was used during fixation. the results obtained show the ... | 2014 | 25509408 |
| [purification of recombinant bacillus cereus resd-rese proteins expressed in escherichia coli strains]. | recombinant e. coli strains expressing the bacillus cereus atcc 14579t resd and resegenes fused with the ubiquitin gene were constructed, and purification of the resd and rese proteins was performed. the approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous resd andrese proteins without denaturation steps up to 150 mg per gram of wet cell weight. | 2014 | 25507776 |
| single cell profiling of surface carbohydrates on bacillus cereus. | cell surface carbohydrates are important to various bacterial activities and functions. it is well known that different types of bacillus display heterogeneity of surface carbohydrate compositions, but detection of their presence, quantitation and estimation of variation at the single cell level have not been previously solved. here, using atomic force microscopy (afm)-based recognition force mapping coupled with lectin probes, the specific carbohydrate distributions of n-acetylglucosamine and m ... | 2015 | 25505137 |
| a common active site of polyhydroxyalkanoate synthase from bacillus cereus yb-4 is involved in polymerization and alcoholysis reactions. | polyhydroxyalkanoate (pha) synthase from bacillus cereus yb-4 (pharcyb4) catalyzes not only pha polymerization but also alcoholytic cleavage of pha chains. the alcoholysis activity of pharcyb4 is expressed when a hydroxyacyl-coa monomer is absent but an alcohol compound is present. in this study, we performed alanine mutagenesis of the putative catalytic triad (cys(151), asp(306), and his(335)) in the phacyb4 subunit to identify the active site residues for polymerization and alcoholysis activit ... | 2015 | 25503319 |
| microbiome of the placenta in pre-eclampsia supports the role of bacteria in the multifactorial cause of pre-eclampsia. | this study was aimed at detecting, identifying, quantifying and comparing the bacteria present in the placental tissues of women with pre-eclampsia with that of normotensive pregnant women. | 2015 | 25492799 |
| bacillus cereus in personal care products: risk to consumers. | bacillus cereus is ubiquitous in nature and thus occurs naturally in a wide range of raw materials and foodstuffs. b. cereus spores are resistant to desiccation and heat and able to survive dry storage and cooking. vegetative cells produce several toxins which on ingestion in sufficient numbers can cause vomiting and/or diarrhoea depending on the toxins produced. gastrointestinal disease is commonly associated with reheated or inadequately cooked foods. in addition to being a rare cause of sever ... | 2015 | 25482451 |
| reducing activity, glucose metabolism and acid tolerance response of bacillus cereus grown at various ph and oxydo-reduction potential levels. | bacillus cereus is a major foodborne bacterial pathogen able to survive a large number of physical-chemical stresses. b. cereus encounters different ph and redox potential (eh7) levels during its passage through the gastrointestinal tract. analysis of the combined influence of ph and redox stresses on b. cereus f4430/73 physiology found that b. cereus f4430/73 growth at ph 7.0 at 37 °c had strong reducing capacities, with a total change of 315 mv from an initial redox value of +214 ± 17 mv. the ... | 2015 | 25475301 |
| genetic discrimination of foodborne pathogenic and spoilage bacillus spp. based on three housekeeping genes. | bacillus genus includes foodborne pathogenic and spoilage-associated species, such as bacillus cereus, bacillus licheniformis, bacillus subtilis and bacillus pumilus. bacillus is also a heterogeneous genus that includes closely related species that are difficult to discriminate among, especially when well-conserved genes such as 16s rrna and 23s rrna are considered. the main goal of the present work was to study the usefulness of three housekeeping genes, the tu elongation factor (tuf), the dna ... | 2015 | 25475298 |
| a case of intoxication due to a highly cytotoxic bacillus cereus strain isolated from cooked chicken. | outbreaks of bacillus cereus infection/intoxication are not commonly reported because symptoms are often mild, and the disease is self-limiting. however, hypervirulent strains increase health risks. we report a case, which occurred in argentina, of severe food poisoning illness on a healthy adult woman associated to b. cereus strain mvl2011. the studied strain was highly cytotoxic, showed high ability to detach caco-2 cells and was positive for the hbla, hblb, and hblc genes of the hbl complex, ... | 2015 | 25475284 |
| novel polycarboxylate porphyrins: synthesis, characterization, photophysical properties and preliminary antimicrobial study against gram-positive bacteria. | we describe the synthesis, characterization and photophysical properties of two new polycarboxylic photosensitizers. owing to their structural design, these two compounds show water solubilities larger than natural carboxylic photosensitizers (e.g., protoporphyrin ix, hematoporphyrin, etc.) and also good singlet oxygen quantum yields. these compounds were tested as photo-antimicrobial agents against staphylococcus aureus and bacillus cereus strains. results reveal that their photocytotoxicities ... | 2015 | 25475206 |
| detection of toxigenic bacillus cereus strains isolated from vegetables in mexico city. | bacillus cereus can cause diarrhea and emetic syndromes after ingestion of food contaminated with it. this ability is due to the production of enterotoxins by this microorganism, these being the hemolysin bl complex, which is involved in the diarrheal syndrome, and cereulide, which is responsible for the emetic syndrome. the detection of genes associated with the production of these toxins can predict the virulence of strains isolated from contaminated food. in this paper, we analyzed 100 sample ... | 2014 | 25474064 |
| surfactins in natto: the surfactin production capacity of the starter strains and the actual surfactin contents in the products. | surfactin-type lipopeptides are suspected of being implicated in the rare food poisonings caused by bacillus species outside the bacillus cereus cluster. in order to get information on surfactin levels in actual human foods, bacilli from three commercial samples of a japanese traditional bean product, natto, were isolated in order to clarify their potential to produce the suspect lipopeptides. the isolated bacilli were characterized as bacillus subtilis. they were β-hemolytic and gave a positive ... | 2014 | 25474063 |
| microbiological quality of seasoned roasted laver and potential hazard control in a real processing line. | microbiological quality of laver, one of the edible seaweeds, has not been reported in a real processing line. laver or supplements were collected from six manufacturers (a to f) to assess potential microbiological hazards and the critical control points in commercial processing lines. aerobic plate counts (apc), coliform counts, bacillus cereus, staphylococcus aureus, and vibrio parahaemolyticus were enumerated, and the presence of b. cereus, listeria monocytogenes, salmonella, s. aureus, and v ... | 2014 | 25474052 |
| effect of white mustard essential oil on the growth of foodborne pathogens and spoilage microorganisms and the effect of food components on its efficacy. | antimicrobial preservative compounds are added to foods to target specific pathogens and spoilage organisms. white mustard essential oil (wmeo) is an extract that contains 4-hydroxybenzyl isothiocyanate, a compound which has been demonstrated to have antimicrobial activity in limited studies. the objective of this research was to determine the in vitro antimicrobial activity of wmeo against gram-positive and gram-negative spoilage and pathogenic bacteria and determine the effect of food componen ... | 2014 | 25474051 |
| modeling of bacillus cereus growth in brown rice submitted to a combination of ultrasonication and slightly acidic electrolyzed water treatment. | the combined effects of ultrasonication and slight acidic electrolyzed water were investigated to improve the microbial safety of brown rice against bacillus cereus infection and to evaluate the growth kinetics of these bacteria during storage of untreated and treated rice at various temperatures (5, 10, 15, 20, 25, 30, and 35°c). the results indicate that this combination treatment was bactericidal against b. cereus, resulting in an approximately 3.29-log reduction. although b. cereus can be ef ... | 2014 | 25474049 |
| prevalence, genetic diversity, and antibiotic resistance of bacillus cereus isolated from korean fermented soybean products. | bacillus cereus contamination is a major food safety problem for korean fermented soybean products, but few studies have assessed its potential to cause foodborne illness. the objectives of this study were to investigate the prevalence and characteristics of b. cereus isolated from korean fermented soybean products. b. cereus was detected in 110 of 162 (67.9%) samples. the highest b. cereus frequency was observed in deonjang (68 of 93 samples, 73.1%) and cheonggukjang (18 of 25, 72.0%); however, ... | 2015 | 25472031 |
| the analysis of distribution of multidrug resistant pseudomonas and bacillus species from burn patients and burn ward environment. | infections caused by multidrug resistant bacteria act as a risk factor for mortality in burns patients. so keeping in view the crucial importance of reliable therapeutic decisions of multidrug resistance bacteria and role of hospital environment in bacteria colonization, our study is based on the evaluation of distribution of pseudomonas sp. and bacillus sp. in burn patients and burn ward environment. | 2015 | 25468476 |
| effect of packaging technology on microbiological and sensory quality of a cooked blood sausage, morcela de arroz, from monchique region of portugal. | morcela de arroz (ma), a popular portuguese blood sausage, with high ph and water activity (aw), is traditionally commercialized without preservatives and unpacked. this study evaluated the best packaging solution to extend ma shelf life stored at 4±1°c for 44days: without packaging (wp), vacuum (vp) and modified atmosphere packaging (map) (80% co2; 20% n2). mesophilic (mtvc), psychrotrophic (ptvc), lactic acid bacteria (lab), pseudomonads, molds and yeasts, enterobacteriaceae, listeria monocyto ... | 2015 | 25462380 |
| integrative approach to produce hydrogen and polyhydroxybutyrate from biowaste using defined bacterial cultures. | biological production of hydrogen (h2) and polyhydroxybutyrate (phb) from pea-shell slurry (pss) was investigated using defined mixed culture (mmc4, composed of enterobacter, proteus, bacillus spp.). under batch culture, 19.0lh2/kg of pss (total solid, ts, 2%w/v) was evolved. using effluent from the h2 producing stage, bacillus cereus egu43 could produce 12.4% (w/w) phb. dilutions of pss hydrolysate containing glucose (0.5%, w/v) resulted in 45-75lh2/kg ts fed and 19.1% (w/w) of phb content. und ... | 2015 | 25460994 |
| aerobic biodegradation of odorous dimethyl disulfide in aqueous medium by isolated bacillus cereus gigan2 and identification of transformation intermediates. | a novel, flagellated, rod-shape, gram-positive facultative aerobe, was isolated and identified as bacillus cereus gigan2. it can effectively remove model odorous organics dimethyl disulfide (dmds) in aqueous solution under aerobic conditions. initial concentration, ph value and temperature played important role in dmds biodegradation, and up to 100% of 10mgl(-1) of dmds could be removed within 96h under the optimum conditions (30°c, ph 7.0 and 200rpm) with a maximum biodegradation rate constant ... | 2015 | 25459868 |
| antibacterial and cytotoxic potential of silver nanoparticles synthesized using latex of calotropis gigantea l. | the present study aimed to synthesis silver nanoparticles (agnps) in a greener route using aqueous latex extract of calotropis gigantea l. toward biomedical applications. initially, synthesis of agnps was confirmed through uv-vis spectroscopy which shows the surface plasmonic resonance peak (spr) at 420 nm. fourier transform infrared spectroscopy (ftir) analysis provides clear evidence that protein fractions present in the latex extract act as reducing and stabilizing bio agents. energy dispersi ... | 2015 | 25459618 |
| an emetic bacillus cereus outbreak in a kindergarten: detection and quantification of critical levels of cereulide toxin. | a bacillus cereus-related emetic outbreak was reported in a belgian kindergarten. high levels of emetic b. cereus (>1.5e+07 colony-forming units/g) were detected in the food leftovers, and the presence of an emetic strain was confirmed in feces. emetic toxin levels ranging up to 4.2 μg/g were also quantified in the leftovers by liquid chromatography coupled to tandem mass spectrometry (lc-ms(2)) analysis. those levels, although moderate in comparison with earlier published intoxications, provoke ... | 2015 | 25457101 |
| brex is a novel phage resistance system widespread in microbial genomes. | the perpetual arms race between bacteria and phage has resulted in the evolution of efficient resistance systems that protect bacteria from phage infection. such systems, which include the crispr-cas and restriction-modification systems, have proven to be invaluable in the biotechnology and dairy industries. here, we report on a six-gene cassette in bacillus cereus which, when integrated into the bacillus subtilis genome, confers resistance to a broad range of phages, including both virulent and ... | 2015 | 25452498 |
| association of a zn(2+) containing metallo β-lactamase with the anticancer antibiotic mithramycin. | pathogenic bacteria that are resistant to β-lactam antibiotics mostly utilize serine β-lactamases to degrade the antibiotics. current studies have shown that different subclasses of metallo β-lactamases (e[mbl]) are involved in the defense mechanism of drug resistant bacteria. here we report that the zn(2+) containing subclass b1 e[mbl] from bacillus cereus binds to a naturally occurring anti-cancer drug mithramycin (mtr). spectroscopic (cd and fluorescence) and isothermal titration calorimetry ... | 2015 | 25450021 |
| establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in saccharomyces cerevisiae via β-alanine. | microbial fermentation of renewable feedstocks into plastic monomers can decrease our fossil dependence and reduce global co2 emissions. 3-hydroxypropionic acid (3hp) is a potential chemical building block for sustainable production of superabsorbent polymers and acrylic plastics. with the objective of developing saccharomyces cerevisiae as an efficient cell factory for high-level production of 3hp, we identified the β-alanine biosynthetic route as the most economically attractive according to t ... | 2015 | 25447643 |
| phospholipase c from pseudomonas aeruginosa and bacillus cereus; characterization of catalytic activity. | to study characteristics of phospholipases c (plcs), their importance for producing microorganisms as well as the potential of their use for industrial purposes. | 2014 | 25441984 |
| antibacterial activity and genotypic-phenotypic characteristics of bacteriocin-producing bacillus subtilis kku213: potential as a probiotic strain. | the antimicrobial activity and probiotic properties of bacillus subtilis strain kku213, isolated from local soil, were investigated. the cell-free supernatant (cfs) of a kku213 culture containing crude bacteriocins exhibited inhibitory effects on gram-positive bacteria, including bacillus cereus, listeria monocytogenes, micrococcus luteus, and staphylococcus aureus. the antibacterial activity of the cfs precipitated with 40% ammonium sulfate (as) remained even after treatment at 60 and 100 °c, a ... | 2015 | 25440998 |
| probabilistic exposure assessment model to estimate aseptic-uht product failure rate. | aseptic-ultra-high-temperature (uht) products are manufactured to be free of microorganisms capable of growing in the food at normal non-refrigerated conditions at which the food is likely to be held during manufacture, distribution and storage. two important phases within the process are widely recognised as critical in controlling microbial contamination: the sterilisation steps and the following aseptic steps. of the microbial hazards, the pathogen spore formers clostridium botulinum and baci ... | 2015 | 25440556 |
| [purification of recombinant bacillus cereus resd-rese proteins expressed in escherichia coli strains]. | recombinant e. coli strains expressing the bacillus cereus atcc 14579t resd and resegenes fused with the ubiquitin gene were constructed, and purification of the resd and rese proteins was performed. the approach used in the study allowed us to increase the protein yield of the electrophoretic homogeneous resd andrese proteins without denaturation steps up to 150 mg per gram of wet cell weight. | 2014 | 25434178 |
| pharmacological basis for the medicinal use of linum usitatissimum (flaxseed) in infectious and non-infectious diarrhea. | linum usitatissimum, commonly known as flaxseed has traditionally been used for the management of diarrhea and gastrointestinal infections. this study was planned to assess pharmacological basis for the medicinal use of flaxseed in infectious and non-infectious diarrhea. | 2015 | 25433250 |
| production and purification of anti-bacterial biometabolite from wild-type lactobacillus, isolated from fermented bamboo shoot: future suggestions and a proposed system for secondary metabolite onsite recovery during continuous fermentation. | wild-type lactobacillus isolated form khorisa, a fermented bamboo shoot product of assam, india were evaluated for production anti-bacterial secondary biometabolites, against staphylococcus aureus. submerged fermentation technique was used for the production of secondary anti-microbial biometabolite by a single wild-type lactobacillus strain, which tested positive for the release of anti-bacterial factor(s). crude cell-free supernatant was obtained, followed by extraction in water-immiscible sol ... | 2015 | 25432345 |
| in vitro antioxidant, antimicrobial and anti-proliferative activities of purple potato extracts (solanum tuberosum cv vitelotte noire) following simulated gastro-intestinal digestion. | analyses of antioxidant and in vitro antimicrobial and anti-proliferative activities of anthocyanin-rich extracts from purple potatoes, solanum tuberosum l. cv vitelotte noire (solanaceae), were performed by simulating both a domestic cooking process and human digestion. extracts of crude and cooked purple potato did not exhibit antimicrobial activity against the tester strains: bacillus cereus, escherichia coli and pseudomonas aeruginosa. the behaviour changed after the simulated gastrointestin ... | 2015 | 25420792 |
| kawasaki disease-specific molecules in the sera are linked to microbe-associated molecular patterns in the biofilms. | kawasaki disease (kd) is a systemic vasculitis of unknown etiology. the innate immune system is involved in its pathophysiology at the acute phase. we have recently established a novel murine model of kd coronary arteritis by oral administration of a synthetic microbe-associated molecular pattern (mamp). on the hypothesis that specific mamps exist in kd sera, we have searched them to identify kd-specific molecules and to assess the pathogenesis. | 2014 | 25411968 |
| comparison of 3 selective media for enumeration of bacillus cereus in several food matrixes. | in this study, we compared the inclusivity, exclusivity, recoverability, and selectivity of the 3 selective agars (mannitol yolk polymyxin b agar [mypa], polymyxin pyruvate egg yolk mannitol bromothymol blue agar [pemba], and brillance bacillus cereus agar [bbc agar]) for bacillus cereus (b. cereus) from pure culture and several food samples. bbc agar showed greater exclusivity and selectivity in pure culture and in foods with high background flora, respectively; however, all the tested media sh ... | 2014 | 25399752 |
| infrared decontamination of oregano: effects on bacillus cereus spores, water activity, color, and volatile compounds. | infrared (ir) heating, a novel technology for decontaminating oregano, was evaluated by investigating the reduction of inoculated bacillus cereus spores and the effect on water activity (a(w)), color, and headspace volatile compounds after exposure to ir treatment. conditioned oregano (a(w) 0.88) was ir-treated in a closed heating unit at 90 and 100 °c for holding times of 2 and 10 min, respectively. the most successful reduction in b. cereus spore numbers (5.6 log units) was achieved after a ho ... | 2014 | 25393824 |
| evaluation of antibacterial and anthelmintic activities with total phenolic contents of piper betel leaves. | the study was conducted to investigate the antibacterial and anthelmintic activities and to determine total phenolic contents of methanolic extract of piper betel leaves. | 2014 | 25386394 |
| infection of cardiovascular implantable electronic devices: detection with sonication, swab cultures, and blood cultures. | the number of patients with cardiovascular implantable electronic devices (cieds) is increasing. the purpose of this study was to compare three methods (sonication, swab cultures, and blood cultures) to detect bacteria in infections of cieds. | 2015 | 25377386 |
| bacillus subtilis hj18-4 from traditional fermented soybean food inhibits bacillus cereus growth and toxin-related genes. | bacillus subtilis hj18-4 isolated from buckwheat sokseongjang, a traditional korean fermented soybean food, exhibits broad-spectrum antimicrobial activity against foodborne pathogens, including bacillus cereus. in this study, we investigated the antibacterial efficacy and regulation of toxin gene expression in b. cereus by b. subtilis hj18-4. expression of b. cereus toxin-related genes (groel, nhea, nhec, and entfm) was downregulated by b. subtilis hj18-4, which also exhibited strong antibacteri ... | 2014 | 25359543 |
| divergence of protein-coding capacity and regulation in the bacillus cereus sensu lato group. | the bacillus cereus sensu lato group contains ubiquitous facultative anaerobic soil-borne gram-positive spore-forming bacilli. molecular phylogeny and comparative genome sequencing have suggested that these organisms should be classified as a single species. while clonal in nature, there do not appear to be species-specific clonal lineages, excepting b. anthracis, in spite of the wide array of phenotypes displayed by these organisms. | 2014 | 25350501 |
| synthesis, characterization and antibacterial activity of biodegradable films prepared from schiff bases of zein. | pure zein is known to be very hydrophobic, but is still inappropriate for coating and film applications because of their brittle nature. in an attempt to improve the flexibility and the antimicrobial activity of these coatings and films, chemical modification of zein through forming schiff bases with different phenolic aldhydes was tried. influence of this modifications on mechanical, topographical, wetting properties and antimicrobial activity of zein films were evaluated. the chemical structur ... | 2014 | 25328181 |
| lifesaving liver transplantation for multi-organ failure caused by bacillus cereus food poisoning. | bacillus cereus is a spore-forming, gram-positive bacterium that causes food poisoning presenting with either emesis or diarrhea. diarrhea is caused by proteinaceous enterotoxin complexes, mainly hemolysin bl, non-hemolytic enterotoxin (nhe), and cytotoxin k. in contrast, emesis is caused by the ingestion of the depsipeptide toxin cereulide, which is produced in b. cereus contaminated food, particularly in pasta or rice. in general, the illness is mild and self-limiting. however, due to cereulid ... | 2015 | 25323120 |
| plant growth promoting bacteria from cow dung based biodynamic preparations. | indigenous formulations based on cow dung fermentation are commonly used in organic farming. three biodynamic preparations viz., panchagavya (pg), bd500 and 'cow pat pit' (cpp) showed high counts of lactobacilli (10(9) ml(-1)) and yeasts (10(4) ml(-1)). actinomycetes were present only in cpp (10(4) ml(-1)) and absent in the other two. seven bacterial isolates from these ferments were identified by a polyphasic approach: bacillus safensis (pg1), bacillus cereus (pg2, pg4 pg5), bacillus subtilis ( ... | 2014 | 25320439 |
| immunomodulatory effect of non-viable components of probiotic culture stimulated with heat-inactivated escherichia coli and bacillus cereus on holoxenic mice. | competition of probiotic bacteria with other species from the intestinal microbiota involves different mechanisms that occur regardless of probiotics' viability. the objective of this paper was to assess the cytokine serum levels in holoxenic mice after oral administration of non-viable components (nvc) of enterococcus faecium probiotic culture stimulated with heat-inactivated escherichia coli and bacillus cereus in comparison to nvc of unstimulated e. faecium probiotic culture. | 2014 | 25317114 |
| an in vitro antibacterial and antifungal effects of cadmium(ii) complexes of hexamethyltetraazacyclotetradecadiene and isomers of its saturated analogue. | to evaluate the antibacterial and antifungal effects of cadmium(ii) complexes with hexamethyltetraazacyclotetradecadiene ligands. | 2014 | 25312179 |
| chemical compositions and antimicrobial activities of athrixia phylicoides dc. (bush tea), monsonia burkeana (special tea) and synergistic effects of both combined herbal teas. | to determine the chemical compositions and evaluate the antimicrobial activity of bush tea (athrixia phylicoides dc.), special tea (monsonia burkeana) and synergy (combination of bush tea and special tea). | 2014 | 25312166 |
| antibacterial activity of ethyl acetate and aqueous extracts of mentha longifolia l. and hydroalcoholic extract of zataria multiflora boiss. plants against important human pathogens. | to determine the potential antibacterial activity of ethyl acetate and aqueous extracts from mentha longifolia l. (m. longifolia) and hydroalcoholic extract of zataria multiflora boiss. (z. multiflora) against important human pathogens. | 2014 | 25312118 |
| enterotoxigenic profiling of emetic toxin- and enterotoxin-producing bacillus cereus, isolated from food, environmental, and clinical samples by multiplex pcr. | bacillus cereus comprises the largest group of endospore-forming bacteria and can cause emetic and diarrheal food poisoning. a total of 496 b. cereus strains isolated from various sources (food, environmental, clinical) were assessed by a multiplex pcr for the presence of enterotoxin genes. the detection rate of nhea, entfm, hblc, and cytk enterotoxin genes among all b. cereus strains was 92.33%, 77.21%, 59.47%, and 47.58%, respectively. enterotoxigenic profiles were determined in emetic toxin- ... | 2014 | 25311736 |
| chemical composition and antimicrobial activity of polish herbhoneys. | the present study focuses on samples of polish herbhoneys (hhs), their chemical composition and antimicrobial activity. a gas chromatography-mass spectrometry (gc-ms) method was used to analyse eight samples of herbal honeys and three samples of nectar honeys. their antimicrobial activities were tested on selected gram-positive (bacillus cereus, staphylococcus aureus, staphylococcus schleiferi) and gram-negative (escherichia coli) bacteria, as well as on pathogenic fungi candida albicans. ether ... | 2015 | 25308646 |
| inhibition of bacillus cereus strains by antimicrobial metabolites from lactobacillus johnsonii crl1647 and enterococcus faecium sm21. | bacillus cereus is an endospore-forming, gram-positive bacterium able to cause foodborne diseases. lactic acid bacteria (lab) are known for their ability to synthesize organic acids and bacteriocins, but the potential of these compounds against b. cereus has been scarcely documented in food models. the present study has examined the effect of the metabolites produced by lactobacillus johnsonii crl1647 and enterococcus faecium sm21 on the viability of select b. cereus strains. furthermore, the ef ... | 2014 | 25305011 |
| draft genome sequence of ft9, a novel bacillus cereus strain isolated from a brazilian thermal spring. | a bacillus cereus strain, ft9, isolated from a hot spring in the midwest region of brazil, had its entire genome sequenced. | 2014 | 25301660 |
| in vivo antipyretic, antiemetic, in vitro membrane stabilization, antimicrobial, and cytotoxic activities of different extracts from spilanthes paniculata leaves. | the study was conducted to evaluate the in vitro antimicrobial activity, cytotoxic, and membrane stabilization activities, and in vivo antiemetic and antipyretic potentials of ethanolic extract, n-hexane and ethyl acetate soluble fractions of spilanthes paniculata leaves for the first time widely used in the traditional treatments in bangladesh. | 2014 | 25299748 |
| increased activity of goat liver plasma membrane alkaline phosphatase upon release by phosphatidylinositol-specific phospholipase c. | mammalian alkaline phosphatase (alp) is attached to the plasma membrane by a unique glycosylphosphatidylinositol (gpi) anchor. the influence of such a complex anchoring device on the enzyme function is not fully understood. here, we report the effect of cleavage of the gpi anchor on the activity of goat liver plasma membrane alp (glpm-alp). phosphatidylinositol-specific phospholipase c (pi-plc) purified from bacillus cereus was used for the cleavage of the gpi anchor (delipidation) and hence for ... | 2014 | 25296497 |
| biological control of meloidogyne hapla using an antagonistic bacterium. | we examined the efficacy of a bacterium for biocontrol of the root-knot nematode (rkn) meloidogyne hapla in carrot (daucus carota subsp. sativus) and tomato (solanum lycopersicum). among 542 bacterial isolates from various soils and plants, the highest nematode mortality was observed for treatments with isolate c1-7, which was identified as bacillus cereus based on cultural and morphological characteristics, the biolog program, and 16s rrna sequencing analyses. the population density and the nem ... | 2014 | 25289015 |
| purification and characterization of a mucin specific mycelial lectin from aspergillus gorakhpurensis: application for mitogenic and antimicrobial activity. | lectins are carbohydrate binding proteins or glycoproteins that bind reversibly to specific carbohydrates present on the apposing cells, which are responsible for their ability to agglutinate red blood cells, lymphocytes, fibroblasts, etc. interest in lectins has been intensified due to their carbohydrate specificity as they can be valuable reagents for the investigation of cell surface sugars, purification and characterization of glycoproteins. the present study reports the purification, charac ... | 2014 | 25286160 |
| thiolation-enhanced substrate recognition by d-alanyl carrier protein ligase dlta from bacillus cereus. | d-alanylation of the lipoteichoic acid on gram-positive cell wall is dependent on dlt gene-encoded proteins dlta, dltb, dltc and dltd. the d-alanyl carrier protein ligase dlta, as a remote homolog of acyl-(coenzyme a) (coa) synthetase, cycles through two active conformations for the catalysis of adenylation and subsequent thiolation of d-alanine (d-ala). the crystal structure of dlta in the absence of any substrate was observed to have a noticeably more disordered pocket for atp which would expl ... | 2014 | 25285205 |
| [bacillus cereus septicemia and necrotizing fasciitis in a patient with liver cirrhosis: a case report]. | a 54-year-old woman with hematemesis was referred to our hospital. she had a history of liver cirrhosis and diabetes mellitus. after inserting a sengstaken-blakemore tube, we performed endoscopic variceal ligation for ruptured esophageal varices. on the third day of admission, she developed septicemia and necrotizing fasciitis caused by bacillus cereus. she was successfully treated with early debridement of both lower extremities and intravenous treatment with vancomycin, ciprofloxacin, and clin ... | 2014 | 25283231 |
| antioxidant and antibacterial activities of exopolysaccharides from bifidobacterium bifidum wbin03 and lactobacillus plantarum r315. | the objective of this study was to investigate the antioxidant and antibacterial activities of exopolysaccharide (eps) from bifidobacterium bifidum wbin03 (b-eps) and lactobacillus plantarum r315 (l-eps). the 1,1-diphenyl-2-picrylhydrazyl (dpph)-radical scavenging, hydroxyl radical-scavenging, and superoxide radical-scavenging abilities were measured to evaluate antioxidant activity. inhibition of erythrocyte hemolysis and lipid peroxidation was also measured. both b-eps and l-eps had strong sca ... | 2014 | 25282420 |
| prevalence and characterization of foodborne pathogens from australian dairy farm environments. | the ability of foodborne pathogens to gain entry into food supply systems remains an ongoing concern. in dairy products, raw milk acts as a major vehicle for this transfer; however, the sources of pathogenic bacteria that contaminate raw milk are often not clear, and environmental sources of contamination or the animals themselves may contribute to the transfer. this survey examined the occurrence of 9 foodborne pathogens in raw milk and environments of 7 dairy farms (3 bovine, 3 caprine, and 1 ... | 2014 | 25282417 |
| genomic comparison of 93 bacillus phages reveals 12 clusters, 14 singletons and remarkable diversity. | the bacillus genus of firmicutes bacteria is ubiquitous in nature and includes one of the best characterized model organisms, b. subtilis, as well as medically significant human pathogens, the most notorious being b. anthracis and b. cereus. as the most abundant living entities on the planet, bacteriophages are known to heavily influence the ecology and evolution of their hosts, including providing virulence factors. thus, the identification and analysis of bacillus phages is critical to underst ... | 2014 | 25280881 |