| pulmonary angiotensin-converting enzyme antienzyme antibody. | a method has been developed for quantitating anticatalytic activity in antibody preparations made in goats against pure solubilized angiotensin-converting enzyme from rabbit pulmonary membranes. anticatalytic activity was purified about 90-fold from a single batch of serum by a procedure including diethylaminoethylcellulose chromatography and elution from sepharose columns containing covalently bound pure enzyme. antiholoenzyme antibody was fractionated with respect to charge and binding affinit ... | 1976 | 186101 |
| serological evidence of the occurrence of bluetongue in iraq. | precipitating antibodies against bluetongue were detected in sheep and goat serum samples collected from animals slaughtered in baghdad abattoir. out of 294 sheep serum samples and 110 goat serum samples examined, 28 and 18 samples respectively showed precipitating activity. in addition, examination of sheep serum samples collected from localities where clinical cases similar to bluetongue were previously reported revealed the presence of bluetongue precipitating antibodies in 101 sera out of 19 ... | 1978 | 208202 |
| a rapid and specific method for separation of bound and free antigen in radioimmunoassay systems. | this communication reports a method for increasing the speed of separation of bound and free antigen in radioimmunoassay systems with no loss in the specificity of binding. the technique uses a mixture of second antibody and polyethylene glycol. it is not species or antibody specific, and systems using specific first antibodies from rabbit, goat or sheep are all functional. results for the assay of parathyrin, calcitonin and corticotropin are described here, although the system has been shown to ... | 1979 | 220374 |
| intrinsic and chemically produced microheterogeneity of staphylococcus aureus enterotoxin type c. | staphylococcus aureus enterotoxins c1 (sec1) and c2 (sec2) produced from 50-liter quantities of crude culture supernatants were purified chromatographically in a neutral or acid milieu. microheterogenity of sec1 was markedly increased by treatment of the purified toxin with alkali, and new more acidic charged species appeared. sec2 was more heterogenous than any of the other s. aureus enterotoxins and was affected only slightly by treatment with alkali. prolonged incubation of the organism durin ... | 1975 | 237837 |
| clinical biochemistry of epilepsy. ii. observations on two types of epileptiform convulsions induced in rabbits with corticotropin. | we propose than an alarm mechanism is operative in animals, designed to regulate neuromuscular irritability by regulating [ca2+]. epinephrine or corticotropin (acth), injected intramuscularly into animals, causes a hypercitricemia, resulting in decreased [ca2+]. this increases muscular excitability to facilitate escape. to avoid over reaction, [cl-] is shifted into the plasma without a concomitant shift of na+, thus generating an acidosis and an increase in ionization of ca. plasma ph, pco2, tot ... | 1979 | 221137 |
| serologic evidence of venezuelan equine encephalitis in some wild and domestic populations of southern texas. | more than 2,500 sera from approximately 30 wild and domestic species in southern texas were tested for neutralizing antibodies to venezuelan equine encephalitis (vee). virus isolations were also attempted from blood and tissue samples of many of the wild specimens. vee neutralizing substances were present in a variety of species collected prior to the 1971 epizootic, suggesting that vee was present and perhaps enzootic in this area before the recent epizootic. serologic results of this study sug ... | 1975 | 238050 |
| studies on stimulation and properties of antiglobulin antibodies. ii. antiglobulin antibodies against homologous globulins and antigens of thermally denatured autologous sera in the sera of rabbits immunized with various antigens. | sera of 48 rabbits after long-term immunization with suspensions of diplococcus pneumoniae, klebsiella friedländeri, proteus ox19 and solution of bovine albumin were examined for antibodies reacting with homologous gamma-globulins and antigens of thermally denatured autologous sera. appearance of antibodies of this type was related to duration of immunization, but not related to the type of antigen used. the experimental results were explained as a result of altered antigenic properties of antib ... | 1975 | 238486 |
| transplantation in severe combined immunodeficiency disease with hl-a identical bone marrow. | the immunologic reconstitution of 7 patients with severe combined immunodeficiency disease was attempted with bone marrow transplantation from histoidentical donors. four patients were successfully reconstituted and discharged from the hospital. two patients died with sepsis. one patient died from a preexisting neurologic disease. all the patients who have been successfully reconstituted have had some degree of graft-vs-host disease. a dose of 50 x 10(6) nucleated bone marrow cells per kg seems ... | 1975 | 238684 |
| isolation and culture of endothelial cells from the lungs of small animals. | techniques are descirbed for the isolation and culture of endothelial cells from the lungs of small animals. the cells are collected by retrograde perfusion of blood-free lungs with buffered saline containing collagenase. the cells are characterized by light microscopy, electron microscopy of thin sections and surface replicas, and by the presence of angiotensin-converting enzyme (ace). ace was assayed using 3h-benzoyl-phe-ala-pro as substrate and was localized by indirect immunofluorescence usi ... | 1979 | 222181 |
| the existence during gestation of an immunological buffer zone at the interface between maternal and foetal tissues. | in mammalian pregnancy the trophoblast normally constitutes an uninterrupted boundary of foetal tissue in immediate contact with maternal tissue, including blood in some species, and is the decisive immunological barrier to rejection of the foetus as an allograft. the ability of the trophoblast to function as a barrier evidently results from its capacity to resist immunological attack by either alloantibody or alloimmune cells and to prevent immunocompetent cells from reaching and damaging the f ... | 1975 | 239427 |
| endocrine factors in genetic improvement of milk production. | the endocrine role in lactation is undisputed, but attempts to correlate milk production with various endocrine products have had limited success. recent work has suggested that placental hormones, in particular placental lactogen, may be an important regulator of lactation capacity. in addition to concentrations of hormones in blood, it is important to consider receptors in target tissue for the hormones. the concentration of receptors in a tissue may vary with genotype of the individual or und ... | 1979 | 222822 |
| folic acid conjugase from plasma. i. partial purification and properties. | human plasma conjugase was partially purified by ammonium sulphate and deae-cellulose column fractionation. the ph optimum was found to be 4.5. the enzyme followed normal michaelis-menten kinetics. the km value was found to be 1.4 x 10(-7)m for folylheptaglutatmate assuming this compound to be the predominant form of folate in yeast extract. the enzyme required sulfhydryl compounds for full activity. differential microbiological assay of the conjugase reaction product using l. casei and s. faeca ... | 1975 | 240785 |
| chimaerism of immunocompetent cells in allogeneic bone marrow-reconstituted lethally irradiated chickens. | injection of parental bone marrow cells into 12-day-old lethally irradiated f1 hybrid chickens resulted in chimaerism of donor-type graft-versus-host (gvh)-reactive cells and suppression of antisheep red blood cell antibody response. these manifestations of a chronic graft-versus-host reaction were prevented by pretreatment of the donor marrow with specific anti-t cell globulin. in some chimaeras donor-type gvh-reactive cells developed gradually from t cells precursors of donor origin. transplan ... | 1975 | 241144 |
| folic acid conjugase from plasma. iii. use of the enzyme in the estimation of folate activity in foods. | a convenient method for the estimation of food folates using human plasma as a source of folic acid conjugase has been standardized. bovine, goat and dog plasma could not be used for the purpose because of low enzyme activity and slow reaction rates. human plasma conjugase released food folates from conjugated forms over a ph range of 4.5 to 6.0. the food folate values obtained by employing rat liver and chicken pancreas conjugase closely agreed with the values obtained by using human plasma con ... | 1975 | 241729 |
| electron microscopic mapping of proteins bound to herpes simplex virus dna. | exonuclease digestion experiments have suggested that there is a protein(s) bound close to one or both ends of herpes simplex virus-1 (hsv) dna. the existence of such bound proteins has been positively demonstrated and their positions on the hsv genome determined by application of a newly developed method for electron microscopic mapping of proteins bound to nucleic acids. purified hsv dna was treated with dinitrofluorobenzene under conditions that covalently attach the dinitrophenyl (dnp) group ... | 1979 | 226938 |
| 'aspira': a new sensitive assay for the detection of hepatitis b surface antigen. | a sensitive method for the detection of hepatitis b surface antigen (hbsag) is described whereby the separation of specific antibody by affinity chromatography is incorporated into one of the assay reagents. hbsag, which may be obtained from tissue culture fluids of a human hepatoma cell line, is first reacted with polystyrene beads. unoccupied spaces on the plastic are next covered with a non-specific protein (e.g. albumin). the plastic is then reacted with either whole antiserum or an igg frac ... | 1979 | 230261 |
| optical properties of bilirubin-serum albumin complexes in aqueous solution. a comparison among albumins from different species. | | 1975 | 242257 |
| an abnormal estrual pattern in a goat (a case history). | | 1977 | 242890 |
| thiamylal sodium anesthesia in the goat. | | 1977 | 243338 |
| [cysticercus in animals slaughtered in the port-bouet abattoir (abidjan)]. | | 1978 | 232291 |
| canine pulmonary angiotensin-converting enzyme. physicochemical, catalytic and immunological properties. | antiontensin-converting enzyme (peptidyldipeptide hydrolase, ec 3.4.15.1) has been solubilized from canine pulmonary particles and purified to apparent homogeneity. a value of approx. 140000 was estimated for the molecular weight of the native and the reduced, denatured forms of the enzyme. no free nh2-terminal residue was detected by the dansylation procedure. carbohydrate accounted for 17% of the weight of the enzyme, and the major residues were galactose, mannose and n-acetylglucosamine with ... | 1978 | 208622 |
| suppression of chemical (den) carcinogenesis in swr/j mice by goat antibodies against endogenous murine leukemia viruses. | | 1978 | 213784 |
| ketamine and xylazine anesthesia in the goat. | | 1978 | 249136 |
| viral leukoencephalomyelitis in two minnesota goats. | | 1978 | 251362 |
| examination of bucks for breeding soundness. | | 1978 | 252347 |
| mastectomy in a goat (a case report). | | 1978 | 252359 |
| hemivertebra in a goat. | | 1979 | 255013 |
| presence of nadph-cytochrome p-450 reductase in rat liver golgi membranes. evidence obtained by immunoadsorption method. | light golgi fractions (gf(1+2)) prepared from rat liver homogenates by a modification of the ehrenreich et al. procedure (j. cell biol. 59:45) had significant nadph-cytochrome p(450) reductase (nadph-cyt c reductase) activity if assayed immediately after their isolation. an antibody raised in rabbits against purified microsomal and golgi fractions. to find out whether this activity is located in bona fide golgi elements or in contaminating microsomal vesicles, we used the following 3-step immuno ... | 1978 | 214451 |
| viral leukoencephalomyelitis in a goat in michigan. | | 1979 | 256362 |
| a survey of caseous lymphadenitis and its etiology in goats in the united states. | | 1979 | 260549 |
| prevention and control of mastitis in dairy goats. | | 1979 | 260575 |
| serological studies with the virus of peste des petits ruminants in nigeria. | it was possible to distinguish separate serological responses when experimental goats were inoculated with either rinderpest virus or peste des petits ruminants virus. examination of field samples established that peste des petits ruminants occur commonly in nigerian sheep and goats although some villages have escaped recent infection. there was no evidence of infection with rinderpest in these animals. | 1979 | 262608 |
| [the fine structure of hemal nodes in goats, with special reference to the passage of intranodal migration of erythrocytes]. | | 1979 | 262771 |
| [brucellosis in goats in san luis province]. | a study on goat brucellosis has been done in the province of san luis, a semidesertic area (annual precipitation ca. 500 ml) located in the center west of argentina. flocks were selected by an statistical method. blood samples were obtained from young and adult females set apart for reproduction. sera were submitted to a fast plate agglutination test with a standard antigen. titres were expressed in international units per ml, accorded to the recommendations of the centro panamericano de zoonosi ... | 1979 | 263179 |
| path analysis: application in an epidemiological study of echinococcosis in new zealand. | the method of path analysis is described in detail. application of this analyogical systems is demonstrated using data from an epidemiological study of echinococcosis in new zealand. the results identified the major causal pathways determining echinococcus granulosus prevalence in dogs, based on multiple regression analysis of a linear causal model constructed from prior biological and epidemiological knowledge. only ethnic and sheep husbandry variables had important direct effect on prevalence ... | 1977 | 265339 |
| epidemiological analysis of factors influencing rate of progress in echinococcus granulosus control in new zealand. | the factors influencing the rate of progress in echinococcus granulosus control in new zealand were analysed by hydatid control area using stepwise multiple regression techniques. the results indicated that the rate of progress was related positively to initial e. granulosus prevalence in dogs and the efficiency with which local authorities implemented national control policy, and negatively to the maori proportion in the local population and the number of dogs per owner. problems in analysis of ... | 1977 | 265340 |
| chemically modified porous silica gel as a bioadsorbent and a biocatalyst. | | 1979 | 232385 |
| antibodies to estrogen receptor: immunochemical similarity of estrophilin from various mammalian species. | immunoglobulin obtained from the serum of rabbits immunized with a highly purified preparation of estradiol-receptor complex from calf uterine nuclei has been shown to contain specific antibodies to the receptor protein (estrophilin) by four criteria: (a) precipitation of the radioactive steroid upon addition of goat antibody against rabbit gamma globulin to a mixture of the tritiated estradiol-receptor complex and the immunoglobulin, (b) adsorption of the estradiol-receptor complex by the immun ... | 1977 | 269423 |
| immunochemical evidence for glutamine-mediated degradation of glutamine synthetase in cultured chinese hamster cells. | the specific activity of glutamine synthetase in cultured chinese hamster cells is inversely related to the concentration of glutamine in the surrounding solution. enzyme specific activity increases 8- to 10-fold when glutamine is removed from serum-free f12 growth media. the induction of glutamine synthetase activity occurs only after glutamine removal and not after the removal of other amino acids (methionine, leucine, or isoleucine). the analysis of the glutamine-mediated decrease in glutamin ... | 1975 | 234454 |
| abnormal cellular localization of thyroglobulin mrna associated with hereditary congenital goiter and thyroglobulin deficiency. | the goiters in a breed of hypothyroid goats contain only minute amounts of thyroblobulin-related antigens (0.01% of normal value). we have analyzed these goiters for the presence of mrna coding for thyroglobulin. using dna complementary to beef 33s thyroglobulin mrna as a probe, we found that the mrna sequence is present in the goat goiter but at a concentration 1/10-1/40 that of normal goat thyroid. hybrids of cdna with either goiter or normal thyroid rna exhibited identical sharp melting curve ... | 1978 | 272675 |
| selective complexing of the "nuclear" 5s estradiol receptor by a serum component, 5s-ca. | a component present in several sera interacts selectively with the 5s "nuclear" form of estradiol receptor but not with the 4s "cytosol" form. this component is therefore referred to as "5s-complexing activity (5s-ca)." this selective interaction occurs with 5s receptor from mouse, calf, or rat. in addition, 5s-ca recognizes the nuclear form of the receptor from hypothalamic as well as uterine tissue thus, 5s-ca indicates a general similarity in the chemical nature of 5s nuclear receptors prepar ... | 1978 | 275834 |
| chlorpheniramine-dependent thrombocytopenia. | | 1975 | 234545 |
| reversal of renovascular hypertension by antibodies specific for angiotensin-converting enzyme. | goat antibodies developed against pure rabbit pulmonary angiotensin-converting enzyme (peptidyl dipeptidase, ec 3.4.15.1) were administered intravenously to rats with two-kidney goldblatt hypertension and to normotensive animals. in the hypertensive model these antibodies were associated with a sustained, immune-dependent decrease of blood pressure to normal values. a smaller, but also immune-specific, reduction of blood pressure was observed in the normotensive group. the data suggest that hete ... | 1978 | 214795 |
| beta-adrenoceptors in the pregnant and non-pregnant myometrium of the goat and cow. | the muscle relaxing effect of beta-adrenoceptor agonists was examined without and with the presence of beta-adrenoceptor antagonists in strips from the pregnant and non-pregnant myometrium of the goat and from the pregnant myometrium of the cow. isoprenaline, salbutamol and ritodrine caused a dose-dependent reduction of the spontaneous contractions of the pregnant myometrium and a dose-related and parallel shift to the right of the isoprenaline dose-response curve was obtained with butoxamine bu ... | 1979 | 216231 |
| humoral factor that specifically regulates prothrombin (factor ii) levels (coagulopoietin-ii). | prothrombin (factor ii) was decreased in rabbits by injection of a goat antiserum to prothrombin. when plasma from these rabbits was injected into normal animals, circulating prothrombin increased in the recipients, suggesting transfer of a humoral regulating factor ("coagulopoietin-ii"). this coagulopoietin-ii resisted boiling for 20 min. on sephadex g-50 gel filtration, it remained in the retained volume, suggesting a molecular weight of less than 30,000. in an in vitro liver system, prothromb ... | 1979 | 284365 |
| muscle membrane chloride conductance and the myotonic syndromes. | the myotonic syndrome of delayed relaxation in striated muscle associated with repetitive membrane electrical activity is characteristic of a number of hereditary disorders in animals and man. this state of pathologic membrane hyperexcitability is also seen as a consequence of intoxication with several classes of chemicals. in most of these states an increase in muscle membrane resistance due to a specific reduction in sarcolemmal gcl can be demonstrated. computer simulations have indicated that ... | 1978 | 285855 |
| complement-dependent cytotoxic antitumor antibody. ii. quantitative determination of cell-bound immunoglobulin m1. | a method for the quantitative determination of rabbit igm bound to cell surfaces has been developed. this method was based on the ability of goat igg specific for rabbit igm heavy chains to bind 125i-labeled protein a when bound to the antigen. with the use of this technique the production of specific igm antitumor antibodies in new zealand white rabbits after immunization with guinea pig hepatoma cells line-1 and line-10 was followed. differences in the production of igm were observed between t ... | 1979 | 286124 |
| problems of sheep and goats. | | 1978 | 287103 |
| selected mycoplasmosis of goats in the united states. | | 1978 | 287104 |
| isolation of mycoplasma and ureaplasma from sheep and goats in west texas (1970-78). | | 1978 | 287105 |
| mycoplasmosis in arizona goats. | | 1978 | 287107 |
| isolation of mycoplasma from pneumonic lungs of sheep and goats in mexico. | | 1978 | 287108 |
| neuromuscular diseases of domestic animals: a summary of muscle biopsies from 159 cases. | | 1979 | 289312 |
| myotonia in the goat. | | 1979 | 289314 |
| pharmacological receptors of the cerebral arteries of the goat. | 5-hydroxytryptamine (5-ht), norepinephrine (ne), histamine (h) and potassium (k+) chloride induce dose-dependent changes in tension of the isolated middle crerbral artery of the goat. vasopressin produces highly variable responses followed by tachyphylaxis; angiotensin ii is ineffective over a wide dose range. the order of potencies of these vasoactive agents is 5-ht greater than ne greater than h greater than k+. with regard to their ability to induce maximal contractile responses, the order is ... | 1975 | 235340 |
| the presence of collagenase in collagen preparations. | the frequently observed instability of neutral salt solutions of native collagen extracted from various sources and partially purified by standard procedures has been studied by disc electrophoresis in polyacrylamide gel and by electron microscopic examination of segment long spacing crystallites. the phenomenon has revealed time and temperature dependency, ph optima near neutrality, and inhibition by sodium edta and serummin addition, collagen breakdown has been found to be quantitatively relat ... | 1975 | 236042 |
| a veterinary group for goats? | | 1979 | 292904 |
| the occurrence of antibodies to parainfluenza3 virus in sera of some domestic animals of the sudan. | | 1979 | 218672 |
| termination of pregnancy in goats using cloprostenol. | | 1979 | 295100 |
| a veterinarian's glimpse at animal health in the people's republic of china. | | 1979 | 298910 |
| immune response of steers, goats and sheep to inactivated rift valley fever vaccine. | | 1979 | 298921 |
| immunochemical studies of infectious mononucleosis. vi. a radioimmunoassay for the detection of infectious mononucleosis heterophile antibody and antigen. | the coated-tube method of solid-phase radioimmunoassay has been adapted to the detection of heterophile antibodies and antigens of infectious mononucleosis. disposable plastic hemagglutination trays were coated with purified glycoprotein from horse erythrocytes and the subsequent uptake of antibody from test sera was detected by radio-iodinated horse erythrocyte glycoprotein. in a preliminary survey of sera from patients with infectious mononucleosis and sera from controls, the assay proved high ... | 1977 | 299865 |
| galactose-6-phosphate dehydrogenase. purification and partial characterization. | a new enzyme, galactose-6-phosphate dehydrogenase has been purified about 50-fold from goat liver. the enzyme can be distinguished from the nonspecific hexose-6-phosphate dehydrogenase and glucose-6-phosphate dehydrogenase by its high substrate specificity and absolute pyridine nucleotide requirement. in contrast to the hexose-6-phosphate dehydrogenase, this enzyme is located exclusively in the cytoplasmic fraction of the cell. the enzyme is a metalloprotein and is highly sensitive to mercurials ... | 1975 | 236296 |
| the distribution and movements of carbon dioxide, carbonic acid and bicarbonate between blood and milk in the goat. | 1. a-v differences and milk concentrations of respiratory gases, ph, hco3 and h2co3 have been measured in lactating goats and cows. 2. the ph and [hco3 minus] of milk were significantly lower than those of plasma while milk pco2 was virtually identical to that of mammary venous blood. [h2co3+ dissolved co2] was similar in milk and blood. 3. 14-c (from injected [14-c]hco3 minus was found to cross the mammary epithelium in both directions. 14-c also passed across the duct epithelium and since this ... | 1975 | 237118 |
| prevention of spontaneous leukemia in akr mice by passive immunization and type specificity of this protection. | akr mice were given sc injections of goat antiviral immune globulin from birth through 14 or 31 days of age. although the shorter immunization schedule greatly reduced expression of spontaneous leukemia, the longer immunization period was required for essentially complete prevention of leukemia. immune globulin with a high neutralization titer for ecotropic virus provided this protection, whereas antibody with a high neutralization titer for murine xenotropic virus did not. | 1979 | 219284 |
| preparation and characterization of an antiserum to cultured human oat-cell carcinoma cells. | viable cultured oat-cell carcinoma cells were used to immunize a goat. the resulting antiserum contained high titres of anti-normal activity and antibodies to cea. it was also shown, by indirect immunofluorescence, using fluorescein-conjugated rabbit anti-goat ig, to localize at high titres on the surface membranes of human lung cancer cells of 4 different histological types. booster immunizations produced a maximum secondary response one week after 2 weekly injections. the course of each immuni ... | 1978 | 207297 |
| modifications of the immunofluorescence assay for feline leukemia virus group-specific antigens. | observations and minor modifications are presented concerning the immunofluorescence assay for feline leukemia virus (felv) group-specific antigens (gsa) in blood cells of cats. data are given regarding absorption of goat felv gsa antiserum in vivo in cats, absorption of the antiserum in vitro with feline blood cells, and the comparative efficacy of various chemical fixatives in preservation of felv gsa for immunofluorescent staining. the best results were obtained with in vitro absorption of an ... | 1978 | 219732 |
| experimental and theoretical aspects of protein folding. | | 1975 | 237413 |
| comparative micelle structure. iv. the similarity between caprine alphas-casein and bovine alphas-3- casein. | the major component of caprine (goat) alphas-casein has been isolated by deae-and cm-cellulose chromatography in buffers containing urea and 2-mercaptoethanol. the protein has a molecular weight of 25700 as determined by gel filtration on sepharose 6b in guanidine hydrochloride. its composition, asp17, thr14, ser14, glu45, pro18, gly4, ala10, cys2, val12, met4, ile12, leu12, tyr11, phe8, his5, lys22, arg6, trp2 and 7 phosphate residues, is much closer to that of bovine alphas3-casein than to bov ... | 1975 | 237571 |
| acid phosphatases activity in cheese and starters. | the acid phosphatase activity levels in a number of greek cheeses and in cheddar cheeses were found to be unaffected by storage for up to 18 months and 12 months respectively. in cheddar cheese, starter organisms made an insignificant contribution to this activity. studies of acid phosphatase prepared from streptococcus cremoris-lactis ncdo 762 starter cultures showed that the enzyme was of high molecular weight and largely particle-bound. the ph of optimum activity was 5-2 and the enzyme was in ... | 1975 | 237943 |
| effect of liver failure on the response of ventilation and cerebral criculation to carbon dioxide in man and in the goat. | 1. the acid-base state of arterial blood and cerebrospinal fluid, and the ventilatory response to co2, were measured in twelve patients with liver disease. the co2 response was also measured in eight goats before and after the experimental production of liver failure. arterial pco2 and ph, cerebral blood flow and the cerebral metabolic rate for oxygen were also measured in four of the goats while they breathed air and various co2-enriched gas mixtures. 2. liver failure was accompanied by a respi ... | 1975 | 238783 |
| blood oxygen affinity and alveolar ventilation in relation in body weight in mammals. | the validity of the concept relating blood oxygen affinity and alveolar ventilation to body weight in homeothermic mammals was reexamined with blood ph used as the fixed variable. blood po2 at 50% saturation at ph 7.4 (p50(7.4)) and pco2 of oxygenated blood at ph 7.4 (pco2(7.4)) from a variety of homeothermic mammals were determined at 37 degrees c by in vitro equilibration techniques. in some species, arterial pco2, po2, and ph were also measured. pco2(7.4), which was similar to arterial pco2, ... | 1975 | 240284 |
| the autoregulatory capability of galen's rete cerebri and its connections. | the rete, a vascular system between the extra- and intracerebral arteries of certain animals, may have a variety of functions in different species. the calibres of the vessels of the rete and its arteries of supply have been measured under different conditions of blood pressure and paco2. it has been shown that the rete and its afferent arteries behave in an autoregulatory fashion down to a blood pressure of 60 mmhg. they also dilate with a rise in paco2. it has also been found that some parts o ... | 1975 | 241946 |
| effect of volatile fatty acids on water and ion absorption from the goat colon. | the absorption of volatile fatty acids (vfa) and the influence of vfa on the net transport of inorganic electrolytes and water were examined in the temporarily isolated colon of the conscious goat. perfusion of the colon with a solution similar in content to that normally present in the cecal outflow showed that the net absorption of vfa was more rapid than that of any other ion present in the solution. when the vfa were replaced with cl, the net absorption of na and water was reduced nearly two ... | 1975 | 242224 |
| frozen storage of embryos of farm animals: progress and implications. | | 1977 | 244396 |
| dilatation of the uterine cervix. | dilatation of the cervix at parturition necessitates changes in the physical characteristics of cervical collagen and glycosaminoglycans which are probably hormone-dependent. the infusion of prostaglandin f2alpha (pgf2alpha) into the arterial blood supply to uterus and cervix of sheep pregnant for about 125 days resulted in cervical dilatation after 24 hours but this response was inconsistent; one factor affecting the response appeared to be the progesterone status, low circulating progesterone ... | 1977 | 246393 |
| the fetal role in timing the initiation of parturition in the goat. | | 1977 | 246396 |
| effect of cold exposure on mammary gland uptake of fat precursors and secretion of milk fat and carnitine in the goat. | | 1979 | 256169 |
| influence of cholinergic receptors on cerebral blood flow of the goat. | | 1977 | 268813 |
| hemoglobin switching in sheep and goats: induction of hemoglobin c synthesis in cultures of sheep fetal erythroid cells. | synthesis of hbf (alpha2gamma2) is replaced by synthesis of hb a (alpha2betaa2) shortly before birth in sheep homozygous for the betaa globin chain whereas hb c (alpha2betac2) is produced transiently during the neonatal period. we have obtained a hb f-to-hb c switch by generating erythroid colonies at high erythropoietin concentration in plasma clot cultures of mid-gestation fetal bone marrow or liver. furthermore, high erythropoietin concentration appeared specifically to activate the gene for ... | 1977 | 270742 |
| goat milk in infant feeding. | | 1978 | 276718 |
| localization of acetylcholine receptor in excitable membrane from the electric organ of torpedo: evidence for exposure of receptor antigenic sites on both sides of the membrane. | nicotinic acetylcholine receptor was localized in a receptor-rich membrane preparation from the electric organ of torpedo californica by applying an immunoferritin technique. the membrane preparation was incubated with (fab')2 fragments derived from specific rabbit antibodies against the purified acetylcholine receptor and subsequently with ferritin-conjugated goat antiserum to rabbit immunoglobulin. more than 50% of the vesicles were found to be labeled with ferritin while the rest remained unl ... | 1978 | 276886 |
| roux-y intestinal bypass for administration of sorbents in uremia. | in order to minimize interaction of sorbents with food and digestive secretions, an intestinal bypass was created for sorbent administration in normal and uremic rats (n = 18) and goats (n = 5). two separate limbs of small intestine were fashioned, one for food absorption and one for sorbent function, which joined at a roux-y anastomosis before the cecum. particulate sorbent suspensions were injected into the intestine via a cutaneous stoma, and were excreted with food wastes in the feces. in an ... | 1978 | 278884 |
| aspects on the choice of experimental animals when reproducing missile trauma. | | 1979 | 291246 |
| the histocompatibility complex gla in the goat. | the current status of research on the major histocompatibility complex of the goat (gla) is discussed, as well as its similarities to other species, although some of the results are preliminary. | 1979 | 292353 |
| electron microscopic visualization of n-acetoxy-n-2-acetylaminofluorene binding sites in cole1 dna by means of specific antibodies. | cole1 dna has been allowed to react in vitro with n-acetoxy-n-2-[14c]acetylaminofluorene in the range of 0-15 n-2-[14c]acetylaminofluorene residues bound per molecule of dna, at the c8 of guanine residues. purified rabbit antibodies to both n-2-(guanosine-8-yl)-acetylaminofluorene and native dna that had reacted with n-acetoxy-n-2-acetylaminofluorene were shown by electron microscopy to recognize specifically the acetylaminofluorene-modified cole1 dna. the antibodies bound to dna were visualized ... | 1979 | 293703 |
| a method for radioactive antiglobulin testing with 125i labeled anti-igg. | the present report describes a method for 125i anti-igg antiglobulin testing. the method involves the use of a labeled deae cellulose igg fraction of goat anti-human igg globulin. leukocyte-free red blood cells were used in the test procedure. both the labeled antiserum and the red blood cell suspensions were diluted in normal rabbit serum to prevent non-specific uptake of labeled antiserum by normal, uncoated red blood cells. the results of the study show that the method is highly reproducible ... | 1977 | 300195 |
| relationship of surface immunoglobulin-bearing cells, plasma cells, and tumor development in anaplastic carcinoma-bearing a/j mice. | since the humoral immune response has been shown to be associated with immunological enhancement of tumor growth, the study of surface immunoglobulin-bearing cells and plasma cell antigen (pca)-bearing cells during neoplastic development may provide new approaches to the study of tumor immunology. peripheral blood was collected every other day from normal and carcinoma-bearing mice. lymphocytes obtained by ficoll-hypaque density centrifugation were assayed for immunoglobulin-bearing cells and pc ... | 1977 | 300278 |
| antisera against leukaemia-associated antigens on human lymphocytes. | antisera were raised in rabbits against leukaemic lymphosarcoma (lsl) cells which carried surface markers of both thymus-derived t lymphocytes (t cells) and bone marrow-derived b lymphocytes (b cells). after absorption with leucocytes, erythrocytes and serum proteins from normal individuals, the antisera demonstrated significant complement-dependent cytotoxicity against leukaemic cells from patients with acute lymphoblastic leukaemia (all) (9/11), lsl (7/9) and chronic lymphocytic leukaemia (cll ... | 1977 | 301073 |
| preparation of a phospholipase c-antihuman igg conjugate, and inhibition of its enzymatic activity by human igg. | we labeled igg with phospholipase c, using 1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide. enzymaticactivity of the resulting conjugate was inhibited when it was complexed with human igg, but rabbit or goat igg was not effective in suppressing the enzyme activity. normal erythrocytes were used as substrate for the enzyme, enzymatic activity being assessed by measuring the release of hemoglobin. the substrates for phospholipase c are phospholipids, which are major components of the erythrocyte m ... | 1977 | 301445 |
| studies on dna synthesis in goat mammary organ culture under the influence of various hormones. | a study has been made of the hormones required for stimulation of dna synthesis in organ culture of mammary tissue of goats. on culture in waymouth's medium without added hormones the incorporation of 6-3h-thymidine into dna was low. the addition of insulin caused a high stimulation of thymidine incorporation. however, the addition of cortisol, prolactin, growth hormone, 17beta-estradiol, progesterone alone or in combination had no effect on dna synthesis. the minimal effective concentration of ... | 1977 | 301818 |
| double-antibody radioimmunoassay for factor viii-related antigen. | a plasma protein required for the support of ristocetin-induced platelet aggregation was isolated from antihemophilic factor concentrate and radiolabeled with 125i. a double-antibody radioimmunoassay was developed, with use of specific rabbit anti-viii related antigen serum and goat anti-rabbit globulin. the assay is sensitive, reproducible, and technically simple to perform. values obtained in normal subjects ranged from 0.65 to 1.53 units, similar to our normal range for viii coagulant activit ... | 1977 | 302160 |
| trypanosoma rhodesiense infection in b-cell-deficient mice. | b-cell-deficient c57b1/6j mice (suppressed from birth with goat anti-mu) and controls (treated from birth with normal goat serum) were infected with trypanosoma rhodesiense. there was a significant (p less than 0.01) decrease in duration of survival of the mu-suppressed mice compared with that of controls. whereas both mu-suppressed and control mice had an initial rise in parasitemia of similar magnitude, only the control mice exhibited a subsequent period during which the parasitemia fell to un ... | 1977 | 303620 |
| effect of estradiol and progesterone on udder growth in goats in vivo and lipid synthesis in goat mammary tissue in vitro. | the effect of estradiol-17beta (e) and progesterone (p) and e, p and deprenon (d; an inhibitor of prolactin secretion) treatment on udder growth, colostrum-like formation and on the induction of lipid synthesis in mammary organ culture were studied. administration of e and p for 8-14 days induced udder growth and colostrum formation. intensive mammary growth and udder distention with secretion occurred following hormone treatment. simultaneous administration of d prevented both udder growth and ... | 1977 | 303988 |
| identity of a t-lymphocyte inhibitor with mouse immunoglobulin in the serum of tumour-bearing mice. | a component of the serum of tumour-bearing mice has been shown to be inhibitory to the immunological function of normal mouse t cells. this factor fractionates with monomeric immunoglobulin upon gel filtration. studies were carried out utilizing goat antisera to the major immunoglobulin chains of mouse ig (kappa, gamma, alpha and mu) mixed with the immunoglobulin-rich fraction of serum from normal mice and tumour bearers and passed through immunoadsorbent columns prepared with rabbit anti-goat i ... | 1977 | 305409 |
| inactivation of antigen-responsive clones with antisera specific for igm or igd. | the present study examines the isotype of cell surface immunoglobulins involved in triggering a response to thymus-dependent and thymus-independent antigens. antibodies to immunoglobulin isotypes were used to block the in vitro interaction of receptor igd and igm with antigen. the results indicate that both igd and igm are necessary to trigger a response to tnp-srbc but that only igm is required for responsiveness to tnp-brucella. limiting dilution studies indicate that the inhibition of the imm ... | 1978 | 305934 |
| isolation and characterization of a rabbit t lymphocyte-specific antigen. | rabbit t lymphocytes may be differentiated from b lymphocytes by the presence of a t lymphocyte-specific surface antigen. this unique antigen has been extracted from the plasma membrane of rabbit thymocytes by 3 m kcl. the presence of the antigen in the membrane extract was demonstrated by inhibition of cytotoxicity with goat anti-rabbit t cell serum (ats). the crude membrane extract was fractionated by gel electrophoresis and the fractions containing the t cell antigen identified by inhibition ... | 1978 | 305942 |
| further characterization of the suppressor cells, activated by goat anti-th-b antibody reagent and responsible for enhanced growth of sarcoma 180 in akr mice. | | 1978 | 306887 |
| regulation of delayed-type hypersensitivity. ii. specific suppressor factor for delayed-type hypersensitivity to sheep erythrocytes in mice. | an antigen-specific suppressor factor for delayed-type hypersensitivity (dth) to sheep red blood cells (srbc) in mice is described. lymph node cells and spleen cells from mice injected intravenously with 1 x 10(9) srbc 4 days previously were incubated in vitro for 48 h in culture medium. supernatant obtained from the culture inhibited the induction of dth to srbc in normal mice. it also suppressed the expression of dth in presensitized mice. the suppression is specific as the suppressor factor h ... | 1978 | 306924 |
| a double-antibody radioimmunoassay for serum progesterone using progesterone-3-(o-carboxymethyl) oximino-[125i]-iodo-histamine as radioligand. | a reliable, convenient and economical radioimmunoassay (ria) for serum progesterone has been established and tested. this procedure employs diethyl ether extraction followed by ria utilizing rabbit anti-11 alpha-hydroxyprogesterone 11-hemisuccinyl-bovine serum albumin (progesterone-11 alpha-bsa) serum, progresterone-3-(o-carboxymethyl) oximino-[125i]-iodohistamine (progesterone-3-[125i]) as radioligand and goat anti-rabbit gamma globulin as second antibody. in conjunction with antiprogesterone-1 ... | 1978 | 307289 |