| [three transport permeases of glutamic acid in saccharomyces cerevisiae: specificity and regulation]. | | 1975 | 58615 |
| studies of the complex between transfer rna molecules and complementary anticodons: kinetic and thermodynamic aspects. | | 1975 | 58627 |
| a ziram-resistant strain of saccharomyces cerevisiae with modified glycerokinase activity. | | 1975 | 58632 |
| [polyelectrolytic behavior of saccharomyces cerevisiae rna in a mixed aqueous-organic environment]. | | 1975 | 58638 |
| characterization of a temperature-sensitive mutant of saccharomyces cerevisiae that undergoes uncontrolled protein synthesis. | a mutant of saccharomyces cerevisiae, dw137, isolated after treatment of a wild-type strain with icr-170. the mutant was respiration-deficient and showed abnormal cell division when grown at 30 degrees c. in addition, the mutant was temperature-sensitive and underwent lysis when grown at 37 degrees c. random spore analysis, induced reversion profiles, and complementation analysis indicated that the abnormal phenotypes were under the control of a single recessive mutation caused by a base-pair su ... | 1976 | 58707 |
| [experience with using the antibiotic olivomycin for demonstrating yeast chromosomes]. | | 1976 | 59303 |
| rapid nuclear staining method for saccharomyces cerevisiae. | mithramycin was used to stain nuclei in mitotically dividing and sporulating yeast. | 1976 | 59726 |
| a colour reaction for the differentiation of ascomycetous and hemibasidiomycetous yeasts. | seventy yeast strains, representative of twenty-six ascogenous genera, four saprobic hemibasidiomycetous genera and thirteen genera of the cryptococcales were tested for their reaction with the stabilized aromatic diazonium compound, diazonium blue b salt. an aqueous, buffered solution of this compound gave a characteristic red colouration with the colonies of the hemibasidiomycetous species and those cryptococcales characterized by the hemibasidiomycetous cell-wall type. the characteristic colo ... | 1976 | 60081 |
| [experimental studies on a treatment for phalloid intoxication]. | | 1975 | 60887 |
| transfer rna: molecular structure, sequence, and properties. | | 1976 | 60910 |
| uptake of l-proline by histoplasma capsulatum. | the uptake and incorporation of l-proline by yeast cells of the dimorphic zoopathogen histoplasma capsulatum were studied. the amino acid was assimilated in at least two ways: by an active transport system with a km of 1.7 x 10(-5) m and by simple diffusion. the active transport system was sterospecific and severely restricted to neutral aliphatic side-chain amino acids. certain analogues inhibited l-proline uptake and prevented incorporation of the amino acid into cellular constituents. the inh ... | 1976 | 61063 |
| the use of the antibiotic lomofungin for demonstration of nuclei and chromosomes in liver yeast cells and protoplasts. | | 1976 | 61924 |
| effect of polaymines on yeast cell-free protein synthesizing system. i. influence of spermine and spermidine on aminoacyl-trna transfer reaction. | spermine and spermidine added to a saccharomyces cerevisiae cell-free protein synthesizing system increased phenylalanine polymerization reaction several-fold at suboptimal concentration of mg2+ and approximately two-fold at optimal amounts of mg2+. the addition of polyamines greatly stimulated the enzymatic and nonenzymatic binding of phenylalanyl-trna and n-acetylphenylalanyl-trna to ribosomes. the binding of the acetylated derivative was higher than phenylalanyl-trna, however, as it was shown ... | 1976 | 62494 |
| effect of polyamines on yeast cell-free protein synthesizing system. ii. increase stability of cell-free system in the presence of spermine. | the addition of spermine, at concentration which stimulates protein synthesis, to the yeast cell-free system significantly increases the thermal stability of the latter. similar stabilizing effect of polyamine is observed for ribosome-poly u-ac-phe-trna complexes. these results suggest that the stimulatory effect of polyamines on the in vitro protein synthesis might be partly due to the increased stability of ribosomes and ribosome-peptydyl-trna complexes. | 1976 | 62495 |
| nutrition of bacillus brevis atcc 9999, the producer of gramicidin s. | the nutrition of the gramicidin s (gs) producer bacillus brevis atcc 9999 was studied with respect to growth and antibiotic production. in a complex medium containing yeast extract and peptone, only glycerol, d-fructose, and meso-inositol contributed to growth, whereas other hexoses, pentoses, polyols, polysaccharides, and organic acids were inactive. glycerol and fructose were inhibitory to gs formation; inositol had no effect. in chemically defined media containing a mixture of five amino acid ... | 1976 | 62553 |
| the transport of pyruvate in yeast mitochondria. | | 1976 | 64168 |
| [comparison of the properties of mitochondrial and plasmic atpases in the yeast schizosaccharomyces pombe]. | | 1976 | 64180 |
| [additional evidence for the ambivalent role of the argr repressor involved in regulation of arginine metabolism of saccharomyces cerevisiae]. | | 1976 | 64185 |
| study of the biological significance of the methylation of cytochromes c. ii. circular-dichroism spectra analysis of the iso-cytochromes from baker's yeast. | | 1976 | 64205 |
| [the affinity of ornithine carbamyltransferase for arginase in saccharomyces cerevisiae]. | | 1976 | 64211 |
| survival and liquid holding recovery in uv-sensitive strains of saccharomyces cerevisiae after treatment with chemical mutagens and radiation. | two uv-sensitive mutants of saccharomyces cerevisiae rad 3 and rad 6 were tested for sensitivity to x-rays, mms, ems, hno2 and deb. rad 3 mutant is more sensitive than the wild type strain only to hno2 and deb, while rad 6 is cross sensitive both to x-rays and all chemicals tested. liquid holding recovery (lhr) was studied by comparison of cell survival immediately after mutagen treatment and after 5 days of storage in phosphate buffer. lh greatly increases cell survival of rad 3 mutant after de ... | 1976 | 65102 |
| effect of the culture medium and incubation time on auxins production by bacteria isolated from the roots of pine seedlings (pinus silvestris l.). | studies were performed on the effect of culture medium and incubation time on the production of auxins by bacteria. the bacteria studied produced more auxins in the mineral medium containing glucose and tryptophan than in that enriched with casamino acids and yeast extract. the amount of auxins elaborated depended both upon the strain and the age of the culture. some strains produced the largest amounts of these substances after 7 days of incubation while others required a longer period. most of ... | 1976 | 65104 |
| identification of cryptococcus neoformans in cytologic preparations of cerebrospinal fluid. | review of routine papanicolaou-stained cerebrospinal fluid preparations from 13 patients who had meningeal cryptococcosis documented by other methods demonstrated the yeast in 11 cases. special stains greatly facilitated the detection of the organisms in two samples and discriminated them from artifacts. an increased number of cells was present in nine cases. correlation with the clinical data revealed that every patient but one had a malignant lymphoma, most commonly hodgkin's disease. the exce ... | 1977 | 65123 |
| subunits of fatty acid synthetase complexes. comparative study of enzyme activities and properties of the half-molecular weight nonidentical subunits of fatty acid synthetase complexes obtained from rat, human, and chicken liver and yeast. | | 1976 | 65153 |
| cytochemical studies on the localization of methanol oxidase and other oxidases in peroxisomes of methanol-grown hansenula polymorpha. | the localization of methanol oxidase activity in cells of methanol-limited chemostat cultures of the yeast hansenula polymorpha has been studied with different cytochemical staining techniques. the methods were based on enzymatic or chemical trapping of the hydrogen peroxide produced by the enzyme during aerobic incubations of whole cells in methanol-containing media. the results showed that methanol-dependent hydrogen peroxide production in either fixed or unfixed cells exclusively occurred in ... | 1976 | 65162 |
| mitochondrial suppressor of a nuclear pleiotropic respiratory-deficient mutant in the petite-negative yeast schizosaccharomyces pombe [proceedings]. | | 1976 | 65986 |
| evidence that trimethylation of iso-1-cytochrome c from saccharomyces cerevisiae does not alter its functional properties [proceedings]. | | 1976 | 66015 |
| [protein and antigenic spectrum of different species of yeasts of the genus candida]. | the methods of electrophoresis in polyacrylamide gel, immunoelectrophoresis and immunoprecipitation were applied to the study of the protein and antigenic spectra of different species of candida yeasts. it was revealed that for the majority of the yeast species the prevalence of proteins of definite fractions was characteristic. c. albincans displayed a marked (in comparison with the nonpathogenic species) content of proteins of proteins of group b. the most pronounced antigenic properties in c. ... | 1976 | 66829 |
| [remarks on the modifications of cell wall during the sporulation of saccharomyces cerevisiae hansen (author's transl)]. | evolution of cell wall during sporulation was studied by means of scanning electron microscopy and by immunological techniques. experiments were done simultaneously with a strain a/alpha able to sporulate and a strain alpha/alpha unable to sporulate. under such conditions it was possible to clarify whether the changes observed were related to the sporulation or to the culture conditions. cell wall structure modifications during sporulation were not obvious morphologically but have been revealed ... | 1976 | 67748 |
| food factors in urticaria. | | 1976 | 68072 |
| the use of yeast cultures for the detection of environmental mutagens using a fluctuation test. | a microbial fluctuation test, modified for the detection of environmental mutagens has been evaluated using a number of strains of the yeast saccharomyces cerevisiae. auxotrophic diploid cultures of yeast which produce prototrophic colonies by both mitotic gene conversion and mutation have been extensively utilized for the detection and evaluation of chemicals showing genetic activity. a number of the yeast strains utilized were shown to be suitable for use in the fluctuation test although the t ... | 1977 | 68435 |
| binding of the 4 methylumbelliferyl glycosides of alpha-mannobiose and mannotriose to tetrameric concanavalin a: equilibrium and fast kinetic studies using fluorescence quenching. | | 1977 | 68752 |
| mutagenic and lethal effects of alpha-benzene hexachloride, dibutyl phthalate and trichloroethylene in saccharomyces cerevisiae. | saccharomyces cerevisiae strain xv185-14c for reversion studies was used to investigate the genetic activity of alpha-benzene hexachloride dibutyl phthalate and trichloroethylene. the results indicate that none of the three compounds was genetically active when yeast cells were treated in phosphate buffer (ph 7.0) in the absence of metabolic conversion. however, in the presence of the 9000 g supernatant of mice liver homogenate, nadp, glucose-6-phosphate, phosphate buffer (ph 7.4), mgcl2, kcl, t ... | 1977 | 69268 |
| [immunologic properties of various cell fractions of a wild strain and a mutant strain of saccharomyces cerevisiae hansen]. | some fractions of the saccharomyces cerevisiae cell wall have been prepared by the action of helix pomatia juice on intact cells. immunosera were obtained by injecting rabbits with these fractions. immunofluorescence reactions, obtained with these sera, show that some fractions of mannopeptides when extracted from a "smooth-colony" mutant strain, have lost antigenic determinants. | 1977 | 70263 |
| phenothiazine phototoxicity: an experimental study on chlorpromazine and related tricyclic drugs. | a large number of phenothiazines and chemically related tricyclic drugs have been studied with respect to their phototoxic potency. two methods were used, an in vivo technique based on the inflammatory response of the mouse tail after systemic administration of the drug plus uva irradiation, and an in vitro method based on growth inhibition of candida albicans. of 27 commercial tricyclic drugs tested in vivo, the most potent were chlorpromazine and two other chlorinated compounds, prochlorperazi ... | 1977 | 70925 |
| nutritive role of different carbohydrates for the growth and survial of oryzaephilus mercator fauvel (coleoptera). | recent investigations on the nutritive role of different carbohydrates for the survival and growth of oryzaephilus mercator show that fructose, glucose, dextrin and glycogen are of high nutritive value, fructose being even better than natural control diet (wheat + 5% yeast). maltose, soluble starch, mannitol and sorbitol, although support some growth, are not of as good nutritive value as control diet. all pentoses, sorbose, galactose, lactose, raffinose, potato starch, inulin and dulcitol are o ... | 1976 | 71029 |
| biological properties of the cyanide-insensitive respiration of the yeast saccharomycopsis lipolytica. | | 1976 | 71060 |
| [a simple relationship between molecular weight and viscosity of rrna of saccharomyces cerevisiae in water-dimethylsulfoxide mixtures]. | | 1976 | 71069 |
| study of the biological significance of the methylation of cytochromes c. i. thermal, acid and guanidinium hydrochloride denaturations of baker's yeast ferricytochromes c. | | 1976 | 71070 |
| enumerative fluorescent vital staining of live and dead pathogenic yeast cells. | a quantitative technique is presented for differentiating live and dead yeast cells grown in culture through the use of the fluorescent dye acridine orange. the method gives results that correlate well with those of other commonly used vital staining techniques and is free of certain interpretative errors inherent in them. vital staining of yeasts with acridine orange also allows for more precise assessment of the physiological state of individual cells and the culture as a whole. the progressiv ... | 1977 | 71766 |
| frameshifts and frameshift suppressors in saccharomyces cerevisiae. | using icr-170 as a mutagen, we have induced a set of mutations in yeast which exhibit behavior similar to that shown for bacterial frameshift mutations. our genetic study shows that these mutations are polar; the polarity can be relieved by internal suppressors; they revert with acridine half-mustards and are not suppressed by known nonsense suppressors. however, they are suppressed by other dominant external suppressors, which fall into two mutually exclusive groups. five genetically distinct s ... | 1977 | 72702 |
| [dry media on yeast hydrolysates for growing vibrio cholerae]. | | 1977 | 72881 |
| cell antigens recognized by rabbit antibodies specific for oligomannosyl determinants. | rabbit antibodies to cell wall mannans of various microbial strains and their mutants were found to be cross-reactive to cell carbohydrates of mammalian sperm and 4-6-days-old blastocysts. immunochemical studies indicate that oligomers of alpha1 yields to 2, alpha1 yields to 3, alpha1 yields to 6, and probably also alpha yields to 4 linked mannose residues of sperm carbohydrates are available for antibody binding. at least 80 percent of binding activity of a yeast mannan antibody to sperm can be ... | 1977 | 73630 |
| cultural and physiological characteristics of clostridium botulinum type g and the susceptibility of certain animals to its toxin. | strain 89 of clostridium botulinum type g, isolated by gimenez and ciccarelli in 1969, was characterized culturally, biochemically, and toxigenically. it was motile, hemolytic asaccharolytic, weakly proteolytic, lipase and lecithinase negative, and it produced acetic, isobutyric, butyric, and isovaleric acids in peptone-yeast extract-glucose broth. no spores were seen in smears from solid or liquid media. very low levels of toxin were produced in regular broth cultures, but dialysis cultures yie ... | 1977 | 74236 |
| fluorescent staining of fungal nuclei with a benzimidazol derivative. | a direct staining procedure is described for fluorescence microscopy of fungal nuclei, chromosomes and mitochondria. the fluorochrome is a benzimidazol derivative (33258 hoechst) known to bind selectively to deoxyribonucleic acid at neutral ph. the advantages of 33258 hoechst relative to feulgen compounds used previously to stain these structures include a greater intensity of fluorescence, the absence of fading or rapid quenching of the fluorescence, and the omission of acid hydrolysis from th ... | 1978 | 75211 |
| humic-like substances of bacterial origin. i. some aspects of the formation and nature of humic-like substances produced by pseudomonas. | two bacterial strains pseudomonas acidovorans no 26 and pseudomonas sp. no 4 grown in conn and yeast extract-glucose media, or in the media enriched with tyrosine, were found to produce dark brown pigment. it was shown that in the bacterial cultures numerous phenolic and quinone-type compounds were formed and transformed to humic-like polymers. formation of humic-like substances started in the bacterial cells and was accompanied by the presence of phenyloxidases in the bacterial cultures. the ba ... | 1977 | 75666 |
| on the synthesis of serine and homoserine samples asymmetrically labelled with tritium and deuterium in the hydroxymethylene group. | (1r) [1-3h, 2h1] 3-phenylpropanol, the key intermediate in the synthesis of (4r) [4-3h, 2h1] d,l-homoserine and of the (4s)-isomer, is obtained from (1s) [1-2h1] 3-phenylpropanol and (1rs) [1-3h] ethanol upon incubation with yeast alcohol dehydrogenase and nad+; under similar conditions 2-phenylethanol undergoes very small exchange with [1-2h2] ethanol. | 1978 | 75808 |
| [study of pli genes action on the cell wall structure of "saccharomyces cerevisiae" hansen (author's transl)]. | a study of the composition of cell walls extracted from a wild strain and three mutant strains "smooth colony" of saccharomyces cerevisiae had been done. it points out differences in the mannose/glucose ratio and in the concentration of some other components of the cell wall in the mutant strains. the immunological study of the cell wall shows that "smooth colonies" mutants and wild strains differs in their mannoproteins. | 1977 | 76456 |
| [comparison of methods for the quantitative detection of moulds in foods. i. communication: selective staining procedures for the directmicroscopic mould count (author's transl)]. | the most important proposed direct microscopic method for the detection of moulds were critically compared in 9 different nonfluid foods, of which 7 were naturally and 2 were artifically moulded.--neither the rot fragment test, nor the howard mould count or the naoh treatment method proposed by mossel (1975) were satisfying with non-fluid foods.--a significant improvement of the direct microscopic detection in the breed-smear could be achieved by selective product specific staining of the fungi ... | 1978 | 77118 |
| yeast dna polymerases: antigenic relationship, use of rna primer and associated exonuclease activity. | highly purified preparation of dna polymerases a and b from yeast were compared with respect to antigenic relationship, ability to use ribonucleotide primers and associated nuclease activity. the following results were obtained. 1. antiserum directed against dna polymerase a inhibits this enzyme but does not interfere with activity of dna polymerase b or of mitochondrial dna polymerase, nor does it precipitate the latter two enzymes. 2. dna polymerase a is capable of using oligo(ribouridylic aci ... | 1978 | 77222 |
| reverse transcription of trna. | the 3' terminus of trna was enzymatically elongated by an oligo(a) tail. a fragment of dna polymerase i (e. coli) was used in the presence of manganese to phase and synthesize a cleavable primer at the oligo(a)-trna template. when the threedimensional structure of oligo(a)-trna is being unfolded under conditions where the primer is still hybridized at the oligo(a) tail, the dna polymerase i fragment transcribes oligo(a)-trna into dna. reverse transcription is slowed down and its fidelity suspend ... | 1978 | 77522 |
| separation and purification of small quantitites of specific rna species by polyacrylamide gel electrophoresis using prestained rnas as markers. | | 1978 | 77643 |
| binding of trna to reverse transcriptase of rna tumor viruses. | the interaction of trna with the reverse transcriptase (rna-dependent dna polymerase) of mammalian rna viruses, such as moloney murine leukemia virus and simian sarcoma virus, has been studied. whereas the purified reverse transcriptase of mammalian viruses sedimented in glycerol gradients as a globular protein with a molecular weight of 70,000, after interaction with trna the enzyme cosedimented with a protein of 150,000 molecular weight. the twofold increase in molecular weight could be a resu ... | 1978 | 77907 |
| [immunochemical comparison of tryptophanyl-trna-synthetases]. | a fraction of immunoglobulins was isolated from the sera of rabbit immunised by a homogeneous beef pancreas tryptophanyl-trna-synthetase (trsase). the igg fraction was shown to inhibit the enzymatic activity during aminoacylation of yeast trnatrp and tryptophan activation. by using the radioimmunoadsorption technique, the interaction of igg was tested with trsaes from beef pancreas and identical enzymes from other sources (contained in the total preparation). beef liver trsase efficiently inhibi ... | 1978 | 78439 |
| genetic activity of bleomycin: differential effects on mitotic recombination and mutations in yeast. | the genetic effects of the antitumor antibiotic, bleomycin were studied in different strains of saccharomyces cerevisiae. it was observed that the drug induced a high frequency of mitotic recombination and gene conversion. in contrast, it produced only a few mutations from adenine independence to adenine dependence and histidine dependence (a missense mutant) to histidine independence. in the strains carrying ochre-suppressible auxotrophic markers, no prototrophs were induced by this drug. the r ... | 1978 | 79138 |
| [anabolism of catalase in yeast: analysis of a constitutive mutant (proceedings)]. | | 1977 | 79377 |
| diuron and other inhibitors of mitochondrial cytochrome b oxidation in saccharomyces cerevisiae [proceedings]. | | 1977 | 79379 |
| mapping of drug-resistant loci in the coenzyme qh2-cytochrome c reductase region of the mitochondrial dna in saccharomyces cerevisiae [proceedings]. | | 1977 | 79380 |
| therapeutic effect of yeast glucan in mice infected with mycobacterium leprae [proceedings]. | | 1977 | 79384 |
| induction by acetate of the cyanide-insensitive respiration in the yeast saccharomycopsis lipolytica [proceedings]. | | 1977 | 79386 |
| comparative structure of the mitochondrial atpases of the yeasts schizosaccharomyces pombe and saccharomyces cerevisiae [proceedings]. | | 1977 | 79395 |
| influence of dosage on intensity and evolution of the granulomatous response in normal mouse liver and spleen, after intravenous administration of yeast glucan [proceedings]. | | 1977 | 79397 |
| [subcellular localization of catalases in saccharomyces cerevisiae (proceedings)]. | | 1978 | 80183 |
| effects of beta-1,3 glucan upon infection by toxoplasma gondii rh. studies in vivo and in vitro [proceedings]. | | 1978 | 80191 |
| inosiplex, a stimulating agent for normal human t cells and human leukocytes. | the influence of inosiplex upon various in vitro leucocyte assays was studied in normal individuals. it was found that the drug increases the response of bidirectional and unidirectional mixed lymphocyte cultures at concentrations of 200, 300, and 500 microgram/ml. it also significantly increases the percentage of active t rosettes (concentration range: 50 to 500 microgram/ml) and the percentage of autologous red cell t rosettes (concentration: 100 microgram/ml). in contrast, inosiplex did not m ... | 1978 | 80429 |
| inhibition of reverse transcription of 70s and 35s avian myeloblastosis rnas by nonprimer trna's. | we studied the kinetics of the reverse transcription of 70s and 35s rna of avian myeloblastosis virus in the presence and absence of various trna's. all trna's inhibited synthesis. trna's from escherichia coli and yeast exhibited a noncompetitive type of inhibition, i.e., they bound reversibly and randomly and did not alter the affinity of the viral rna for the polymerase. nonprimer trna's obtained from 70s rna molecules produced a complex pattern of inhibition. the results show that the nonprim ... | 1978 | 80459 |
| responses of radiation-sensitive mutants of saccharomyces cerevisiae to lethal effects of bleomycin. | haploid and diploid strains of yeast containing genes conferring radiation-sensitivity were studied under growing and nongrowing experimental conditions for their relative sensitivities to growth-inhibitory effects of bleomycin (bm). the rad1, rad2, rad3, rad4, rad5 (and allelic rev2), rad7, rad10, rad11, rad 12, rad14, rad15, rad16 and rev3 strains exhibited responses similar to normal (rad+) yeast strains. it is concluded from these findings that the excision-repair function deficient in sever ... | 1978 | 80746 |
| [essay of proteins after aldehyde treatment in biological objects (author's transl)]. | following fixation with formalin or glutaraldehyde, the protein content of bovine serum albumin, lysozyme, non-disintegrated yeast cells and liver nuclei of mice is determined by means of a brom-phenol blue staining procedure and the widely used lowry-technique. the bromphenol blue technique permits determinations of soluble as well as particulate proteins or protein mixtures fixed with up to 6% formalin or 2% glutaraldehyde. recovery rates differ no more than 20% as related to unfixed controls. ... | 1978 | 80908 |
| appearance of an electron paramagnetic resonance signal during the induction of the cyanide-insensitive respiration in the yeast saccharomycopsis lipolytica [proceedings]. | | 1978 | 81012 |
| enzyme and genes for asparagine synthesis in saccharomyces cerevisiae [proceedings]. | | 1978 | 81024 |
| regulation of cytochrome c peroxidase anabolism: the role of porphyrins [proceedings]. | | 1978 | 81027 |
| inhibition by rna of rnase h activity associated with reverse transcriptase in rauscher murine leukemia virus cores. | we reported earlier that core preparations of rauscher murine leukemia virus, when separated on an isopycnic sucrose gradient, did not contain detectable levels of rnase h activity, while retaining high levels of reverse transcriptase activity. we reexamined this phenomenon, and the earlier observation was found to be reproducible. however, when doubly banded preparations of viral cores were solubilized and reverse transcriptase was isolated by ion-exchange chromatography, a coincident peak of a ... | 1978 | 81312 |
| respiratory mutation and galactose metabolism in yeast saccharomyces cerevisiae. | restriction in growth on galactose as unique source of energy due to respiratory deficiency resulting from mutation in a gene gal probably different from gal 3 is described. | 1978 | 81593 |
| reaction of trnaphe from yeast with 1-fluoro-2,4-dinitrobenzene. attachment sites of the potential antigenic-determining 2,4-dinitrophenyl residues. | the reaction of 1-fluoro-2,4-dinitrobenzene with trnaphe from yeast, for the introduction of antigenic-determining 2,4-dinitrophenyl residues into trna, took place only at adenosine residues in trnaphe. after reaction at ph 8.0 and 50 degrees c two kinds of products were detected: one was ribose-modified adenosine which was derived from the 3' terminus of trna, and the other was base-modified adenosine. the sites and extent of the modification of each particular adenosine residue of trnaphe were ... | 1978 | 81773 |
| mutagenic and antimutagenic effects of bleomycin in saccharomyces cerevisiae. | | 1978 | 82203 |
| bleomycin-induced mutation and recombination in saccharomyces cerevisiae. | clinical preparations of bleomycins (bm) were tested for their recombinogenicity and mutagenicity at relatively high survival levels in the simple eucaryote, saccharomyces cerevisiae. more than a dozen test loci or genetic intervals were assayed for bleomycin-induced mutation or recombination. treatments of stationary phase diploid yeast routinely results in 25--75% inactivation. the antibiotic was mildly to very highly recombinogenic and mutagenic, with one exception. the amount of bleomycin-in ... | 1978 | 82205 |
| properties of a lipid-stimulated plasma membrane atpase in yeast [proceedings]. | | 1978 | 84577 |
| [fluorescence study of the position of tryptophan in a hydrophobic protein of the yeast inner mitochondrial membrane (proceedings)]. | | 1978 | 84578 |
| biogenesis of coupling site i in the yeast saccharomycopsis lipolytica [proceedings]. | | 1978 | 84580 |
| biochemical properties of mutants of saccharomyces cerevisiae resistant to inhibitors of the mitochondrial cytochrome b oxidation [proceedings]. | | 1978 | 84628 |
| circular dichroism and fluorescence studies of carboxypeptidase y from saccharomyces cerevisiae [proceedings]. | | 1978 | 84631 |
| purification and preliminary characterization of protease b from saccharomyces cerevisiae [proceedings]. | | 1978 | 84637 |
| distinct nuclear genes for yeast mitochondrial and cytoplasmic methionyl-trna synthetases. | | 1978 | 84671 |
| an immunodiffusion activity stain for yeast histidinol dehydrogenase. | | 1979 | 85419 |
| nuclear division in temperature-sensitive multicellular mutants of wangiella dermatitidis. | temperature-sensitive morphological mutants of wangiella dermatitidis were stained with mithramycin, a fluorescent nuclear stain. multicellular forms of the mutants each contained two or more nuclei, documenting that nuclear division accompanied the yeast-to-multicellular form conversion. | 1979 | 86536 |
| [use of a method of staining sections with methyl green-pyronine for histobacterioscopic purposes]. | a method of staining of histological sections with methyl green-pyronine for histobacterioscopic detection of microflora in tissues is proposed. a bright red staining with pyronine of microbial agents and yeast-like fungi of the genus candida due to a high content of rna in them permits elective detection of their insignificant amounts in tissues even at low magnifications of the microscope. | 1979 | 87185 |
| antibodies to germinating and yeast cells of candida albicans in human and rabbit sera. | two major antigenic components, i and ii, were detected by double immunodiffusion in sonic extracts of the germinating (g) or yeast (y) cells of the dimorphis organism, candida albicans group a. component i may be a heterogeneous mixture of antigens which are stable to heating and phenol. component ii is more homogeneous but is labile to heat and phenol. rabbit antisera, showing only precipitin to component ii or certain human sera at high dilution, were found to react with g cells to give an im ... | 1979 | 87404 |
| food allergies and migraine. | 60 migraine patients completed elimination diets after a 5-day period of withdrawal from their normal diet. 52 (87%) of these patients had been using oral contraceptive steroids, tobacco, and/or ergotamine for an average of 3 years, 22 years, and 7.4 years respectively. the commonest foods causing reactions were wheat (78%), orange (65%), eggs (45%), tea and coffee (40% each), chocolate and milk (37%) each), beef (35%), and corn, cane sugar, and yeast (33% each). when an average of ten common fo ... | 1979 | 87628 |
| lactase degradation by human enteric bacteria. | twelve non-pathogenic bacteria and two yeast strains isolated from the duodenal aspirate or mucosa of five children with diarrhoea were tested for their ability to degrade non-human lactase in vitro. both yeast strains and eleven of the bacterial strains significantly reduced lactase activity. a similar action on human lactase could be a cause of lactose intolerance. | 1979 | 88558 |
| enzymic polyadenylation of 5s ribosomal ribonucleic acid and synthesis of a complementary deoxyribonucleic acid. | the 5s ribosomal rna has been isolated, pure and intact, from rat liver (5 mg of 5s rna from 150g of liver). the 5s rna serves as a primer for calf thymus poly(a) polymerase with 20% of the efficiency of (ap)3a. bacterial 5s rna and transfer rna also serve as primers; rat liver 18s and 28s ribosomal rnas support poly(a) synthesis poorly. neither the 5s rna primer nor the appended poly(a) tract is nicked or degraded by poly(a) polymerase, and initiation of poly(a) tracts on 5s rna primers continu ... | 1979 | 88957 |
| autoregulation of germ tube formation by candida albicans. | germ tube formation by candida albicans is at least partially controlled by a product(s) of the yeast phase of the organism which is released from cells upon incubation at 37 degrees c in tissue culture medium or fetal calf serum. this germination regulatory substance is stable under conditions of lyophilization and heating of 70 degrees c, but becomes inactivated at ph values of 4.0 and 9.5. a germination regulatory substance was produced by both strains of c. albicans tested and by a strain of ... | 1979 | 89087 |
| distribution of anionic groups at the cell surface of different sporothrix schenckii cell types. | the distribution of anionic groups at the cell surface of yeastlike forms, hyphae, and conidia of sporothrix schenckii was studied by staining with colloidal iron hydroxide and cationized ferritin. by using colloidal iron hydroxide it was shown that the external cell wall layer of one strain (strain 1099.18) could be resolved into two reactive sublayers and that these layers were present in many but not all cells of the same population. in contrast, most cells of another strain (strain 1099.12) ... | 1979 | 89092 |
| receptor-mediated binding and internalization of leukocyte elastase by alveolar macrophages in vitro. | radioiodinated leukocyte elastase was found to bind rapidly and specifically to alveolar macrophages in vitro. in contrast to the binding of pancreatic and bacterial proteases, leukocyte elastase binding did not require the presence of alpha 2 macroglobulin. the binding was inhibited by an excess of unlabeled enzyme and was saturable by increasing elastase concentrations. leukocyte elastase binding thus met criteria for receptor-mediated binding, with and estimated association constant of 4.97 x ... | 1979 | 89120 |
| oxidation of inorganic sulfur compounds by yeast. | | 1979 | 89797 |
| antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion. | | 1979 | 89825 |
| new method for estimating digestion of paracoccidioides brasiliensis by phagocytic cells in vitro. | we describe a method by which phagocytosis and digestion of paracoccidioides brasiliensis yeast cells by polymorphonuclear leukocytes or other phagocytic cells may be estimated. suspensions of p. brasiliensis in its yeastlike phase were sonicated, counted, and incubated with known numbers of peripheral blood polymorphonuclear leukocytes. at given intervals, cytocentrifuge droplets were stained by a variation of papanicolaou's method. stained preparations were examined with phase-contrast optics. ... | 1979 | 90683 |
| atypical legionella-like organisms: fastidious water-associated bacteria pathogenic for man. | a group of related bacteria designated atypical legionella-like organisms (allo) has been identified. allo, like l. pneumophila, are fastidious gram-negative rods that grow well on charcoal yeast extract (cye) agar and produce ground glass colonies and browning of modified yeast extract agar. unlike l. pneumophila, allo do not grow well on feeley-gorman (fg) agar, and on cye agar they fluoresce under longwave ultraviolet light. allo and l. pneumophila have a similar predominance of branched-chai ... | 1979 | 91024 |
| isolation of lipophilic yeast in "sterile" peritonitis. | | 1979 | 91045 |
| blastomycosis: report of three cases from alberta with a review of canadian cases. | approximately 120 cases of blastomycosis have been reported from canada to-date. the great majority of these occurred in the eastern provinces. since 1970, three cases of blastomycosis have been seen in alberta. the first case, with meningeal and pulmonary involvements, was diagnosed at post-mortem. the second case was that of a 75-year-old male with a history of pancytopenia, aortic arteriosclerosis, exposure to mercury, and fever. koh and periodic-acid schiff (pas) stained smears of the lung t ... | 1979 | 91108 |
| spontaneous and induced non-specific drug resistance in saccharomyces cerevisiae. | nitrous acid, diepoxybutane and methyl methane sulfonate induce effectively non-mitochondrial chloramphenicol-resistant mutants cross-resistant to other drugs. hno2 induces also unstable erythromycin resistant mutants. the ability of the mutants to grow on antibiotic media can be modified by detergents, guanidine hydrochloride or increased osmotic pressure of the medium. this suggests that the resistance is due to changes in cell membrane permeability similar to those described by rank, robertso ... | 1979 | 92168 |