| expression of human papillomavirus type 16 e6 protein by recombinant baculovirus and use for detection of anti-e6 antibodies in human sera. | existing assays to detect antibodies to human papillomavirus type 16 (hpv-16) proteins in sera from cervical carcinoma patients rely primarily on bacterially produced recombinant proteins or synthetic peptides for use as target antigens. these methods have had limited success in the detection of antibodies against the e6 protein. to produce more authentic e6 protein for use in serological assays, we have employed a recombinant baculovirus vector to synthesize the protein in insect cells. cells i ... | 1992 | 1328490 |
| detection of human papillomavirus (hpv) infections in japanese women with and without abnormal cervical cytology by dot blot and southern blot hybridization. | the frequency of human papillomavirus (hpv) was investigated in 245 japanese women (100 showing normal cytology and 145 showing abnormal cytology). a previously presented method for the detection and typing of hpv dna was used, where a dot blot test excluded samples that did not hybridize with hpv dna. positive samples were further analyzed by a southern blot procedure, to give the hpv type. a mixture of subgenomic probes of the types 6, 11, 16, 18, 31, 33 and 35 was used. hpv dna was detected i ... | 1992 | 1328760 |
| [human papillomavirus and intraepithelial cervical cancer after renal transplantation. apropos of 2 observations]. | therapeutic immunosuppression applies to an increasing number of patients, exceeding largely the only transplant recipients. among its detrimental carcinological complications are the uterine cervix intraepithelial neoplasia whose strong relationship with the lesions due to human papillomavirus is well known. they need an early and regular follow-up of the concerned women, by cervical smear tests, colposcopic assessment and histological examinations, to undertake in time the adequate treatment, ... | 1992 | 1328908 |
| human papillomavirus dna detection in primary anogenital warts and cervical low-grade intraepithelial neoplasias in adults by in situ hybridization. | in this study, 58 consecutive patients with primary anogenital warts were selected from patients attending a genitourinary clinic. patients were grouped on the basis of clinical lesion site, i.e. patients with genital warts only, patients with perianal or anal canal warts only, and patients with concurrent perianal and genital warts. of these patients, 38% of the men (12/31) and 33.3% of the women (9/27) had other anogenital infections, such as nonspecific urethritis (nsu) or nonspecific genital ... | 1992 | 1329237 |
| second primary cancers following anal and cervical carcinoma: evidence of shared etiologic factors. | the authors examined the incidence of second primary cancers occurring after cervical and anal cancer. data from the connecticut tumor registry for 1935-1988 and eight other us tumor registries for 1973-1988 were used. women with primary invasive cervical cancer had a relative risk of 4.6 (95% confidence interval (ci) 2.4-8.1) for subsequent invasive anal cancer. increased relative risks after cervical cancer were also found for cancers of the oral cavity (relative risk (rr) = 2.2), stomach (rr ... | 1992 | 1329500 |
| oral contraceptives and human papillomavirus infection in cervical intraepithelial neoplasia. | we report about 142 patients from whom colposcopically directed cervical punch biopsies were taken which showed condylomatous lesions with or without cervical intraepithelial neoplasia (cin). fifty-six (39.4%) of these women used oral contraceptives (oc) for at least two years before examination. we used dna in situ hybridization on all biopsies for detection of human papillomavirus (hpv)-dna. among oc users a significant trend towards higher hpv infection rates in high grade cin (odds ratio 2.9 ... | 1992 | 1329677 |
| detection of human papillomavirus in vulvar carcinoma. a study by in situ hybridisation. | fourty-four specimens of invasive cancers of the vulva, including 38 primary invasive squamous carcinomas, were analysed by in situ hybridisation with biotinylated hpv 6/11, 16 and 18 dna probes. four (9%) of the 44 carcinomas were positive for hpv dna: three (7%) for hpv 16 dna and one (2%) for hpv 6/11 dna. hpv dna was found only in squamous carcinomas. of the 38 primary squamous carcinomas, 11% were positive (8% hpv 16, 3% hpv 6/11). the overall 5-year survival was 48.7%, 48.5% for the squamo ... | 1992 | 1329678 |
| inosine-containing primers in human papillomavirus detection by polymerase chain reaction. | a pcr protocol has been developed using inosine-containing primers for human papilloma virus (hpv) detection. the hpv16 l1 region from caski-positive cells was efficiently amplified by a single reaction and directly analyzed by agarose gel electrophoresis. the method was found to be sensitive and reproducible, and it easy to use for hpv typing and screening. | 1992 | 1330051 |
| [human papillomaviruses and cellular oncogenes (c-myc, c-ha-ras) in cutaneous and mucosal lesions in transplantation recipients]. | transplant recipients develop numerous benign and malignant cutaneous and mucosal lesions in which histological signs of human papillomavirus (hpv) infection are observed. to investigate the role of hpv and c-myc and c-ha-ras cellular oncogenes' activation in transplanted patients lesions, we used in situ hybridization with biotinylated probes and southern blot to detect hpv and oncogenes dna. we analyzed 36 lesions from grafted patients: 11 common warts, 10 actinic keratoses, 13 squamous cell c ... | 1992 | 1330099 |
| oncogenes and tumour-suppressor genes in squamous cell carcinoma of the head and neck. | cancer is now considered to be a multi-hit process which involves a number of aberrant genetic events culminating in malignant transformation. in squamous cell carcinoma (scc) of the head and neck the action of both oncogenes and tumour-suppressor genes has been identified during the course of the disease. cytogenetic analysis of these carcinomas has demonstrated chromosomal breakpoints, particularly in the regions of 1p22 and 11q13 together with frequent amplification of the proto-oncogenes in ... | 1992 | 1330149 |
| sequential combined tumorigenic effect of hpv-16 and chemical carcinogens. | we immortalized oral keratinocytes by transfection with recombinant human papillomavirus type 16 (hpv-16) dna and established two cell lines, human oral keratinocytes-16a (hok-16a) and -16b (hok-16b). these cell lines were morphologically different from the normal counterpart, contained hpv-16 dna as integrated form and expressed numerous viral genes. however, these cells proliferated only in culture medium containing low calcium (0.15 mm) and are not tumorigenic in nude mice. to test the hypoth ... | 1992 | 1330348 |
| identification of a negative regulatory domain in the human papillomavirus type 18 promoter: interaction with the transcriptional repressor yy1. | the human papillomavirus type 18 (hpv-18) promoter contains a tpa responsive element (tre) which confers tpa responsiveness on a heterologous promoter. in the context of the hpv-18 promoter, however, this ap-1 site is inactive. we have identified a negative regulatory domain in the hpv-18 promoter which represses the constitutive and tpa-induced ap-1 activity. this negative regulatory sequence has been mapped to 44 nucleotides (ol13). we identified this element as a transcriptional silencer base ... | 1992 | 1330541 |
| prevalence of genital human papillomavirus infections in patients at a sexually transmitted diseases clinic. | the human papillomavirus was detected in cervicovaginal cells by the polymerase chain reaction in 14 of 37 (37.8%) patients attending a sexually transmitted disease (std) clinic and in 6 of 43 healthy young women (14.0%) undergoing routine gynecologic examinations who served as controls. the results indicated that even the more malignant types of human papillomaviruses were not uncommon among the control group, and that the prevalence of human papillomavirus infection was significantly higher in ... | 1992 | 1330561 |
| verrucous carcinoma of the penis: importance of human papillomavirus typing for diagnosis and therapeutic decision. | one case of penile verrucous carcinoma (buschke-löwenstein tumor) undergoing anaplastic transformation and containing human papillomavirus type 6 is presented. the viral genome is detected by in situ hybridization using biotin-labeled cdna probes. the clinical, histological and virologic criteria of verrucous carcinoma are discussed in comparison to giant condyloma and highly differentiated squamous cell carcinoma. the importance of viral typing determination for further diagnostic and therapeut ... | 1992 | 1330573 |
| [behavior of nucleolar organizers in the normal cervix, human papilloma virus infection and cervico-uterine cancer]. | thirty five cervical biopsies were divided into four groups: 5 cases normal cervix, 10 cases with papillomavirus without atypias (hpvio), 10 cases with human papillomavirus with atypias (hpvia) and 10 cases with squamous carcinoma. with the colloidal silver technique, the proteins associated with nucleolar organizers were stained; they were found to increase significantly in number in the groups studied being lowest in the normal cervix and highest in the biopsies with carcinoma. the number of n ... | 1992 | 1330837 |
| biologic characteristics of specific human papillomavirus types predicted from morphology of cervical lesions. | human papillomavirus (hpv) dna was detected by southern blot hybridization in cervicovaginal lavage samples from 199 of 329 (60.5%) women attending a municipal hospital colposcopy clinic. human papillomavirus was identified in 195 of 264 (73.9%) patients with a squamous intraepithelial lesion or cancer on biopsy or papanicolaou smear (bethesda system) compared with 11 of 65 (16.9%) without squamous intraepithelial lesion (p < .0001). the most common hpv type identified was hpv 16 (20.6% of posit ... | 1992 | 1330876 |
| polymerase chain reaction-based methods for the detection of human papillomavirus dna. | the unprecedented sensitivity of the polymerase chain reaction (pcr) dna amplification technology provides numerous advantages for the detection of human papillomavirus (hpv) dna. in this chapter, the principles of the method and its advantages are discussed; disadvantages of the technology and proposed solutions are also presented. to accommodate the complexity of the many distinct hpv types, both type-specific and consensus pcr-based detection methods have evolved. consensus primer systems, wh ... | 1992 | 1330905 |
| detection of human papillomavirus dna without amplification: prospects for clinical utility. | certain hpv types are major risk factors for the development of cervical and other human cancers. this chapter describes some of the problems which have precluded accurate diagnosis of hpv-related conditions in many studies. despite the confusion, a multitude of reports demonstrate that hpv dna testing has clinical utility, and future investigations should be directed at more accurately delineating its role in human health care. | 1992 | 1330906 |
| measurement errors in epidemiological studies of human papillomavirus and cervical cancer. | measurement errors have been an important concern in studies of human papillomavirus (hpv) and anogenital cancers. misclassification of hpv infection status is a possible explanation for incoherent findings in previous epidemiological studies purporting to show an etiological role for hpv in cervical cancer. even low levels of misclassification of hpv infection can cause severe underestimation of hpv prevalence in field surveys, bias the association between hpv and sexual activity, and impair th ... | 1992 | 1330909 |
| interactions between human papillomavirus and human immunodeficiency virus infections. | an increasing body of information permits certain conclusions to be drawn about the nature and magnitude of the interactions between hpv and hiv infections and their influence on the genesis of intraepithelial neoplasia and, to a lesser extent, cancer. importantly, findings tend to be consistent across a number of independent studies. while hpv infection probably does not significantly alter the course of hiv infection, hiv-induced immunosuppression does increase the severity and duration of ano ... | 1992 | 1330910 |
| relationship between human papillomavirus infection and tumours of anogenital sites other than the cervix. | although tissues in the case series of anal, penile, vaginal and vulvar neoplasms that have looked for evidence of hpv infection by probing for hpv dna have been selected for convenience, they support the view that hpv, especially type 16, is associated with approximately 50% of these tumours. a higher percentage of the anal, vaginal and vulvar tumours are associated with hpv 16 than are penile tumours. this discrepancy may be due to the low number of penile tumours studied or to a true differen ... | 1992 | 1330912 |
| hpv and cervical neoplasia: review of case-control and cohort studies. | epidemiological studies on the association between human papillomavirus (hpv) and cervical cancer are reviewed. studies using hpv dna hybridization methods to assess the presence of viral markers have yielded compelling evidence that hpv has a causal role in the disease: the association is strong, consistent and specific to a limited number of viral types. a dose-response relationship has been reported between increasing estimated viral load and risk of cervical cancer. indirect evidence suggest ... | 1992 | 1330915 |
| screening for cervical cancer: is there a place for incorporating tests for the human papillomavirus? | well organized screening programmes for cervical cancer, based on exfoliative cervical cytology, are known to be effective at reducing the incidence of invasive cervical cancer and mortality from the disease. hpv testing should not replace cervical cytology as the first-line approach in screening for cervical cancer, as hpv testing is not sufficiently reliable and some cancers are not associated with hpv infection. even though there are many unanswered questions about the validity of hpv tests, ... | 1992 | 1330916 |
| detection of human papillomavirus in cervical scrapes by the polymerase chain reaction in relation to cytology: possible implications for cervical cancer screening. | an hpv screening strategy based on general primer-mediated and type-specific pcr is described. a relationship was found between an increasing hpv prevalence rate and a higher degree of cervical dysplasia (up to 100% in cervical carcinoma). based on these prevalence studies in the netherlands and preliminary data showing that progression of cervical lesions is always associated with persistent infection of oncogenic/high-risk hpv types, a cervical cancer screening scheme incorporating pcr-based h ... | 1992 | 1330917 |
| assessment of exposure to sexually transmitted agents other than human papillomavirus. | there are now a number of lines of evidence giving support for a causal role of high-risk oncogenic hpv types in cervical cancer. however, it is clear that only a minority of women with hpv 16 infection, for example, develop invasive cancer. therefore examination of whether sexually transmitted agents other than hpv are interacting in the genesis of cervical neoplasia is necessary. serological methods which accurately reflect past exposure to individual agents are ideal for epidemiological studi ... | 1992 | 1330921 |
| viral co-infections in human papillomavirus-associated anogenital lesions according to the serostatus for the human immunodeficiency virus. | in hiv-infected men, human papillomavirus (hpv) infection is strongly linked with the development of anogenital lesions but is not a sufficient factor to explain the neoplastic transformation of such lesions. we investigated the association between hpv and herpesvirus infections in penile and anal lesions from 54 hiv-seronegative and 54 hiv-seropositive men by means of colposcopy, histopathology and in situ hybridization. our patients showed condyloma acuminata (39%), papular warts (35%) and mac ... | 1992 | 1330931 |
| the causal link between human papillomavirus and invasive cervical cancer: a population-based case-control study in colombia and spain. | to evaluate the association between human papillomavirus (hpv) and cervical cancer, we performed a population-based case-control study in columbia and spain, the former country having an incidence rate of cervical cancer about 8 times higher than the latter. it included 436 cases of histologically confirmed invasive cervical cancer and 387 randomly selected population controls. information on demographic variables, sexual behaviour and other risk factors was obtained by interview. hpv-dna was me ... | 1992 | 1330933 |
| risk factors for cervical cancer in colombia and spain. | a population-based case-control study of cervical cancer was conducted in spain and colombia to assess the relationship between cervical cancer and exposure to human papillomavirus (hpv), selected aspects of sexual and reproductive behaviour, use of oral contraceptives, screening practices and smoking. the study included 436 cases of histologically confirmed squamous-cell carcinoma and 387 age-stratified controls randomly selected from the general population that generated the cases. the presenc ... | 1992 | 1330934 |
| comparative analysis of human papillomavirus detection by dot blot hybridisation and non-isotopic in situ hybridisation. | to determine the relative diagnostic performance of non-isotopic in situ hybridisation (nish) and a dot-blot assay for detecting human papillomavirus (hpv) on exfoliated cervical cells; and to correlate the results with cytopathological assessment. | 1992 | 1331197 |
| genotypes and sequence variants of human papillomavirus dnas from human immunodeficiency virus type 1-infected women with cervical intraepithelial neoplasia. | human papillomavirus (hpv) dna was found in cervicovaginal lavage fluids from 9 of 11 human immunodeficiency virus type 1 (hiv-1)-seropositive female prostitutes with cervical intraepithelial neoplasia (cin) in kinshasa, zaire. since 7 yielded complex nucleic acid hybridization results consistent with mixed hpv infections, limited sequencing of hpv dna was used to identify the hpvs present. three of hpv 16 and 1 each of hpv 18, 31, 33, and 56 and me180-hpv were identified by sequencing in 8 samp ... | 1992 | 1331247 |
| pre- and posttreatment serum antibody responses to hpv 16 e2 and hsv 2 icp8 proteins in women with cervical carcinoma. | serum antibodies to early proteins of human papillomavirus type 16 (hpv 16 e2 protein) and herpes simplex virus type 2 (hsv 2 icp8) can be measured by elisa. in the serum of 122 newly diagnosed cervical carcinoma patients and age-matched controls, enhanced iga antibody levels to an hpv-16 e2 protein derived peptide no. 245 indicated a 9.5-fold (95% confidence limits 2.8-57.2) relative risk of cervical carcinoma. no significant risk was found with a corresponding hpv 6 e2 peptide or hsv 2 icp8. t ... | 1992 | 1331306 |
| the relationship between contraceptives, sexual practices, and cervical human papillomavirus infection among a college population. | four hundred and sixty-five college women were evaluated to determine if specific variables of social and sexual behavior correlated with the presence of human papillomavirus (hpv) dna in the genital tract, and if these associations differed between women who were hpv dna positive, hpv dna positive/clinically (cytologically) negative, or who reported previous hpv-related disease by a history of an abnormal papanicolaou (pap) smear or genital warts. hpv positive women had more sexual partners in ... | 1992 | 1331341 |
| prevalence of human papillomavirus infection in men. comparison of the partners of infected and uninfected women. | men were checked for the presence of genital human papillomavirus (hpv)-associated lesions. the evaluation included acetic acid-guided, high-magnification examination of the penis and scrotum employing a colposcope and biopsy confirmation of hpv-associated lesions in the genital area. the men were in a high-risk group if they were sexual partners (for at least six months) of women known to harbor genital condyloma. the men were in a low-risk group if they were sexual partners of women with no kn ... | 1992 | 1331439 |
| human papillomavirus-negative status and c-myc gene overexpression: independent prognostic indicators of distant metastasis for early-stage invasive cervical cancers. | | 1992 | 1331478 |
| homologous sequences in adenovirus e1a and human papillomavirus e7 proteins mediate interaction with the same set of cellular proteins. | studies of adenovirus e1a oncoprotein mutants suggest that the association of e1a with the retinoblastoma protein (prb) is necessary for e1a-mediated transformation. mutational analysis of e1a indicates that two regions of prb are required for e1a to form stable complexes with the retinoblastoma protein. in addition to prb binding, these regions are necessary for e1a association with several other cellular proteins, including p130, p107, cyclin a, and p33cdk2. here we show that short synthetic p ... | 1992 | 1331501 |
| ultrastructural localization of human papilloma virus by nonradioactive in situ hybridization on tissue of human cervical intraepithelial neoplasia. | a nonradioactive in situ hybridization was developed to localize human papilloma virus (hpv) at the ultrastructural level. | 1992 | 1331611 |
| oral contraceptive use and human papillomavirus infection in women without abnormal cytological results. | both experimental and epidemiological data support the idea that oral contraceptive (oc) use may have a stimulating effect to a certain point on cervical carcinogenesis. the current investigation tries to answer the question whether oc use might have an influence on early human papillomavirus (hpv) infections. a total of 425 women without abnormal cytological results were examined colposcopically, and filter in situ hybridisation (fish) was used to determine the presence of human papillomavirus ... | 1992 | 1331728 |
| [epidemiology of female ano-genital condylomata acuminata. in relation to the presence of specific types of the human papillomavirus]. | the presence of 7 human papillomavirus (hpv) types in genital condylomata acuminata (gca) from 94 otherwise healthy women was detected by in situ hybridization, employing 3 different dna probe cocktails (6/11, 16/18 and 31/35/51). hpv+ and hpv- gca did not differ significantly as to the multiplicity and site of lesions, nor the prevalence of younger age, previous pregnancies or abortions, oral contraception, concurrent vaginal infection, aspecific pap-test abnormalities and cutaneous hypo-anergy ... | 1992 | 1331872 |
| human papillomavirus type 16 in an oral squamous carcinoma and its metastasis. | dna was extracted from fresh frozen tissues of eight patients with primary oral squamous carcinoma. samples of normal oral mucosa were available in seven cases and metastatic tumor in two cases. the samples were probed for human papillomavirus types 16 and 18 by southern hybridization. in one case of squamous carcinoma of the floor of the mouth, human papillomavirus type 16 was identified in the primary tumor and a lymph node metastasis, but it was not detectable in normal oral mucosa from this ... | 1992 | 1331929 |
| human papillomavirus infections. | though the existence of disease associated with hpv has been documented for centuries, it has been only within the past 2 decades that we have recognized the clinical diversity and significant morbidity and mortality associated with hpv infections. the original lack of interest and nonavailability of in vitro culture systems has hampered research. however, with the advent of molecular diagnostic techniques, strong evidence suggests that hpv plays a major role in the development of specific anoge ... | 1992 | 1332449 |
| self-administered home cervicovaginal lavage: a novel tool for the clinical-epidemiologic investigation of genital human papillomavirus infections. | our objective was to evaluate a self-administered cervicovaginal irrigation apparatus (my-pap, medtech, bohemia, n.y.) for the detection of cervicovaginal human papillomavirus infection. | 1992 | 1332474 |
| human papillomavirus genomes in male urethral cells. | sixty-four samples of urethral cells from male sexual partners of women with genital human papillomavirus (hpv) infection were analyzed for the presence of hpv types 6, 11, 16, and 18 by polymerase chain reaction (pcr) followed by slot blot hybridization. additional samples from 37 of these subjects were analyzed for the presence of viral cytopathic effects by conventional cytology. by pcr, hpv dna was detected in 21% (14/64) of samples. by cytology, 16% (6/37) of the samples showed cellular cha ... | 1992 | 1332485 |
| expression of the l2 and e7 genes of the human papillomavirus type 16 in female genital dysplasias. | the expression of the e7 and l2 genes of hpv 16 was studied in benign and precancerous female genital lesions to evaluate their role in the development of dysplasias. ninety biopsy specimens from 70 patients, selected on basis of dot blot dna hybridization, were included in immunohistochemical and in situ hybridization analyses. in the hpv 16 dna positive cases, l2 mrna and e7 mrna were detected in biopsies from 24 and 21 patients, respectively. l2 mrna was found in eight of 16 cases of condylom ... | 1992 | 1332486 |
| in situ hybridization for the detection of human papillomavirus (hpv) in gynaecological biopsies. a study of two commercial kits. | human papillomavirus (hpv) detection in biopsies from the lower genital tract may be requested by clinicians as a complement to ordinary histopathological diagnosis. in the present study, two commercial kits, (enzo diagnostics inc., new york, usa and biohit, helsinki, finland) used for in situ hybridization with biotinylated c-dna probes were compared and the hpv-expression was evaluated in relation to histopathological findings. the enzo kit identifies hpv-types 6/11, 16/18, 18 and 31/33/51, wh ... | 1992 | 1332575 |
| hpv genital infections and contraception. | human papilloma virus genital infections are, especially during fertile age, a very common finding in performing pap smear and colposcopy. the interest in these infections arises from their frequent association with dysplasia and their possible role in the etiology of carcinoma of the lower genital tract. the natural history of hpv infections is not very well known and foreseen. epidemiological studies indicate that the progression, regression or stability of induced hpv lesions are correlated w ... | 1992 | 1332833 |
| human papillomavirus type 16 expresses a variety of alternatively spliced mrnas putatively encoding the e2 protein. | the full-length e2 protein of human papillomavirus type 16 is believed to act as a trans-repressor of the viral p97 promoter. previous reports have provided evidence that transcripts with the potential to encode the e2 protein contain the 880/2708 splice junction. we have further analyzed the structure of the e2-encoding transcripts. employing the rna polymerase chain reaction (pcr) technique and analyses of the rna pcr products by southern blot hybridization and dna sequencing, we revealed the ... | 1992 | 1333130 |
| properties of bovine papillomavirus e1 mutants. | ostensibly comparable mutants of bovine papillomavirus type 1 (bpv-1) affecting the e1 open reading frame that were constructed in several laboratories have been reported to exhibit either reduced or increased transformation efficiencies in established mouse cell lines relative to wild-type bpv-1 dna. to resolve these discrepancies, we have reexamined many of the mutants in mouse c127 cells by using focus formation assays. our primary conclusions are that all e1 mutants tested consistently gener ... | 1992 | 1333131 |
| detection of human papillomavirus dna in the normal cervices of japanese women by the dot-blot (vira pap) method. | exfoliated cervical cells obtained from 9 patients with cervical intraepithelial neoplasia (cin) i and ii, 11 with cin iii, 22 with cervical carcinoma, and 599 with cytologically normal cervices (including 63 pregnant women) were evaluated for the presence of human papillomavirus (hpv) by the dot-blot (vira pap) method. hpv infection was detected in 33.3% of the cin i and ii cases, in 54.5% of the cin iii cases, in 68.2% of invasive cervical carcinoma cases, and in 4.2% of the normal group. the ... | 1992 | 1333184 |
| vulvar and vaginal disorders. | vulvovaginitis is the most common reason for a woman to see her gynecologist. the most common causes are bacterial vaginosis, candida species, and trichomonas vaginals, respectively. human papillomavirus has become a major diagnostic and therapeutic problem. human papillomavirus dna testing suggests that exposure to human papillomavirus occurs frequently at birth and intermittently throughout a woman's life after the onset of sexual activity. interferon has been shown to be effective in treating ... | 1992 | 1333291 |
| two cases of plantar epidermal cyst associated with human papillomavirus. | hpv-associated epidermal cysts of the sole (haecs) of the foot have been reported recently in japan in which there is positive staining for papillomavirus antibody in the nuclei of the epithelial cells in the cyst wall and vacuoles in the stratified horny material inside the cysts. a causative association with a newly recognized hpv, hpv 60, has been recorded. the authors report two new cases. the possible mechanism of cyst formation in hpv 60-infected epithelium is discussed. | 1992 | 1333376 |
| comparison of virapap, southern hybridization, and polymerase chain reaction methods for human papillomavirus identification in an epidemiological investigation of cervical cancer. | in order to provide a reliable diagnosis for the presence and type of human papillomavirus (hpv) dna in a case-control study of cervical cancer in colombia and spain, 926 cervical scrapes from female subjects were examined by virapap (vp) and southern hybridization (sh), and 510 of these (263 cases and 247 controls) were also tested by polymerase chain reaction (pcr) using the hpv l1 consensus primers. hpv dna prevalence was much higher in cases than in controls by each of the three tests. there ... | 1992 | 1333485 |
| [study on the management of dysplasia of the uterine cervix]. | in this study, we investigated problems on the management of dysplasia on the basis of our clinical data obtained in the past ten years. in addition, we also examined the biological significance of protooncogene expression and human papilloma virus (hpv) infection in the initiation and promotion of dysplasia. among 540 cases of dysplasia we have managed, 48.8% of the cases of mild, moderate and severe dysplasia which we followed up for more than 6 months regressed, and 24.1% of the cases progres ... | 1992 | 1333505 |
| natural history of anal cytologic abnormalities and papillomavirus infection among homosexual men with group iv hiv disease. | previous studies have demonstrated a high prevalence of anal cytologic abnormalities as well as anal human papillomavirus (hpv) infection among homosexual men with group iv hiv disease. however, the natural history of these changes in this population has not yet been studied. to this end, 37 homosexual men with group iv hiv disease attending an outpatient hiv clinic were followed at approximately 9-month intervals for an average of 17 months, using anal cytology, anoscopy, anal biopsy, and anal ... | 1992 | 1333531 |
| 'cystic papilloma': human papillomavirus in a palmar epidermoid cyst. | | 1992 | 1333746 |
| pathogenesis of human immunodeficiency virus-related kaposi's sarcoma. | infection with the human immunodeficiency virus-1 is associated with a marked increase in the incidence of kaposi's sarcoma. recent studies suggest that the risk of kaposi's sarcoma in human immunodeficiency virus infection is increased with oral-fecal contact and that a sexually transmitted agent possibly related to human papillomavirus-16 could be involved. exposure to this or another sexually transmitted agent apparently alters both the morphology and growth regulation of the kaposi's sarcoma ... | 1992 | 1333810 |
| tamoxifen stimulates human papillomavirus type 16 gene expression and cell proliferation in a cervical cancer cell line. | the widely adopted use of tamoxifen as a chemotherapeutic agent is primarily based on its inhibition of cancer cell growth. however, we report that tamoxifen at low concentrations (10(-9) and 10(-11) m) causes stimulation of cell proliferation in a cervical cancer cell line, sfr. the facts that sfr cells do not contain estrogen receptors and are estrogen nonresponsive imply the existence of an antiestrogen-specific binding protein and suggest that the effect of tamoxifen is possibly mediated thr ... | 1992 | 1333883 |
| southern blot hybridization and pcr in detection of oral human papillomavirus (hpv) infections in women with genital hpv infections. | the presence of human papillomavirus (hpv) in biopsies taken from clinically normal buccal mucosa (n = 212) and clinical lesions (n = 60) was examined by southern blot hybridization (sbh) using 32p-labelled hpv dna probes. furthermore, one hundred formalin-fixed, paraffin-embedded biopsies were analyzed by using polymerase chain reaction (pcr), combined with dot blot hybridization and biotinylated hpv dna probes. with sbh and pcr, 15.4% and 29.4% of the biopsies, respectively, contained hpv dna. ... | 1992 | 1334147 |
| detection of human papilloma virus type 16 dna in oral squames from normal young adults. | we have employed the polymerase chain reaction (pcr) to detect human papillomavirus (hpv) type 16 in oral squames and mononuclear cells from 62 healthy young adult volunteers. two groups were screened for the presence of this virus, but in not all cases was dna obtained from the scrapes. in the first (n = 30), the results show that 43% of normal individuals harbour hpv 16 (a genital type) in their buccal mucosa, epithelium of dorsum of tongue and hard palate. in the second group (n = 18), 44% of ... | 1992 | 1334148 |
| invasive cervical cancer in young women. clinicopathologic correlation and demonstration of human papillomavirus by in situ hybridization. | it has been suggested that invasive cervical carcinoma is biologically more aggressive in young patients than in older patients. the clinicopathologic characteristics and human papillomavirus (hpv) status of women < or = 35 years of age with invasive cervical carcinoma treated at emory university hospital from 1985-1989, were evaluated in a retrospective study. the group consisted of 23 patients, 35% of all patients with invasive cervical carcinoma. age at diagnosis, clinical stage, histologic c ... | 1992 | 1334150 |
| the e2 binding sites determine the efficiency of replication for the origin of human papillomavirus type 18. | human papillomaviruses (hpv-s) have been shown to possess transforming and immortalizing activity for many different, mainly keratinocyte cell lines and they have been detected in 90% of anogenital cancer tissues, which suggests a causative role in the induction of anogenital and other tumours. we have exploited a quantitative assay to identify and characterize the origin of replication of the human papillomavirus type 18 (hpv-18), one of the most prevalent types in the high-risk hpv group. repl ... | 1992 | 1334259 |
| papillomavirus l1 major capsid protein self-assembles into virus-like particles that are highly immunogenic. | infection by certain human papillomavirus types is regarded as the major risk factor in the development of cervical cancer, one of the most common cancers of women worldwide. analysis of the immunogenic and structural features of papillomavirus virions has been hampered by the inability to efficiently propagate the viruses in cultured cells. for instance, it has not been established whether the major capsid protein l1 alone is sufficient for virus particle assembly. in addition, it is not known ... | 1992 | 1334560 |
| retinoic acid induces secretion of latent transforming growth factor beta 1 and beta 2 in normal and human papillomavirus type 16-immortalized human keratinocytes. | similar cellular responses are elicited by retinoic acid (ra) and transforming growth factor beta (tgf-beta). we investigated the ability of ra to modulate the production of tgf-beta in normal human keratinocytes (hkc) and hkc lines immortalized by transfection with human papillomavirus type 16 dna (hkc/hpv16). ra treatment of both normal hkc and hkc/hpv16 resulted in a 2-3-fold induction in secreted levels of latent tgf-beta. the induction in tgf-beta secretion by ra was dose dependent, with si ... | 1992 | 1334692 |
| expression of the hpv16 e7 gene generates proliferation in stratified squamous cell cultures which is independent of endogenous p53 levels. | monolayer cultures of human foreskin and ectocervical epithelial cells were infected with retroviral vectors expressing hpv16 oncogenes, selected for g418 resistance, and cultured organotypically so that they reformed the fully differentiated, stratified squamous tissues from which they were originally derived. expression of hpv16 e7 prevented cell cycle withdrawal in the suprabasal layers of these stratified cultures but had no effect on terminal differentiation. cultures expressing e7 alone an ... | 1992 | 1334693 |
| human papillomavirus type 16 found in primary transitional cell carcinoma of the bartholin's gland and in a lymph node metastasis. | we report a case of primary transitional cell carcinoma of the bartholin's gland and its lymph node metastasis that contained hpv 16 sequences by polymerase chain reaction. the physical state of hpv 16 dna in the primary cancer was investigated by southern blot analysis which showed the presence of the episomal form of viral dna. our findings of hpv 16 dna in the transitional cell carcinoma of the bartholin's gland and its metastasis would indicate that both tumors arose from a single clonal eve ... | 1992 | 1334938 |
| human papillomavirus type 6 infection involving cutaneous nongenital sites. | human papillomavirus (hpv) type 6 is classically considered a mucosatropic virus. interestingly, clinical manifestations of hpv 6 infection that involve nonmucosal or nongenital sites have rarely been described. the reasons for this site specific infectivity of hpv 6 are unknown. we describe a patient who had condylomata acuminata-like lesions that involved cutaneous nongenital sites; hpv 6 dna was detected in skin biopsy specimens with use of the polymerase chain reaction, followed by hybridiza ... | 1992 | 1334983 |
| detection and typing of human papillomaviruses present in fixed and stained archival cervical smears by a consensus polymerase chain reaction and direct sequence analysis allow the identification of a broad spectrum of human papillomavirus types. | dna well suited for polymerase chain reaction (pcr) amplification was purified from archival papanicolaou smears. the detection of a wide range of human papillomavirus (hpv) types was made possible using a hpv-specific consensus primer pair, and typing was conveniently done by direct sequence analysis of the pcr product. the method could be of unique value in longitudinal and cross-sectional studies aimed at answering a number of fundamental pathological and epidemiological questions regarding h ... | 1992 | 1335027 |
| in vitro infection of normal human keratinocytes by human papillomavirus type 1 followed by amplification of the viral genome in reconstructed epidermis. | primary cultures of normal human keratinocytes were inoculated in vitro with human papillomavirus type 1 (hpv-1), the agent responsible for deep plantar warts. upon transfer to dead de-epidermized dermis and growth at the air-liquid interface, keratinocytes reconstituted a pseudoepidermis. under these highly differentiating conditions, hpv-1 dna amplification was found to take place in the reconstructed epidermis, being detectable from 7 days after the transfer and persisting for at least 10 day ... | 1992 | 1335028 |
| detection of capsid antigen of human papillomavirus (hpv) in benign lesions of female genital tract using anti-hpv monoclonal antibody. | we established a murine monoclonal antibody (k1h8) to human papillomavirus (hpv) using alkaline-disrupted virions of hpv type 1 (hpv-1) as the immunogen. k1h8 recognized a 57 kd capsid protein of hpv-1 and detected the antigen in paraffin sections of formalin-fixed tissue. with k1h8, we examined immunohistochemically 68 biopsy specimens obtained from the female genital tract. the specimens were histologically condyloma acuminatum or koilocytotic lesions with or without dysplasia and each specime ... | 1992 | 1335045 |
| practical tips for assessment and management of vulvar and vaginal human papillomavirus. | human papillomavirus (hpv) infection has been strongly associated with intraepithelial neoplasia and cancers of the lower genital tract. due to either better detection or increased incidence of this infection, there is a greater demand for colposcopy. historically, clinicians have focused their colposcopic investigation on the cervix, often neglecting evaluation of the vulva and vagina. because hpv is a multicentric disease, these areas should not be overlooked. nurse colposcopists need to asses ... | 1992 | 1335318 |
| costimulation of antitumor immunity by the b7 counterreceptor for the t lymphocyte molecules cd28 and ctla-4. | interaction of the b7 molecule on antigen-presenting cells with its receptors cd28 and ctla-4 on t cells provides costimulatory signals for t cell activation. we have studied the effects of b7 on antitumor immunity to a murine melanoma that expresses a rejection antigen associated with the e7 gene product of human papillomavirus 16. while this e7+ tumor grows progressively in immunocompetent hosts, cotransfection of its cells with b7 led to tumor regression by a b7-dependent immune response medi ... | 1992 | 1335364 |
| distribution of human papillomavirus 16 in the blood of women with uterine cervix carcinoma. | in the present study hpv 16 dna was discovered in the cervical cancer tissue and in blood of women with cancer. in the blood of patients with advanced cancer the hpv appeared in granulocytes and was anchored to the lymphocytic membrane. it may be therefore concluded that the presence of hpv 16 dna in the blood of women with cervical cancer reduced immunocompetence. | 1992 | 1335412 |
| prognostic significance of polymerase chain reaction detected human papillomavirus of tumors and lymph nodes in surgically treated stage ib cervical cancer. | this study describes the prognostic role of polymerase chain reaction detected human papillomavirus (hpv) in stage ib cervical cancer patients treated with radical hysterectomy and pelvic and paraaortic node dissection. all tumors were confined to the cervix and all margins and nodes were disease free. twenty-one patients were analyzed: 6 patients recurred within 20 months of initial therapy, while 15 had no evidence of disease with a minimum follow-up of 36 months. polymerase chain reaction (pc ... | 1992 | 1335431 |
| epidermodysplasia verruciformis as a model of human papillomavirus-induced genetic cancers: the role of local immunosurveillance. | epidermodysplasia verruciformis (ev) presents a genetically determined, unusual susceptibility to infection with ev-specific human papillomaviruses (hpvs) related to abrogation of immunosurveillance exclusively against these viruses. the cutaneous viral carcinogenesis depends upon potentially oncogenic hpvs, the cocarcinogenic effect of ultraviolet irradiation, and genetic host factors, presumably a defect of anti-oncogenes or alleles of major histocompatibility complex and tumor necrosis factor ... | 1992 | 1335684 |
| lymphoproliferative responses to human papillomaviruses in patients with cutaneous warts. | in vitro lymphoproliferative responses of peripheral blood mononuclear cells (pbmc) from patients with cutaneous warts, caused by infection with human papillomavirus type 1 (hpv-1) or type 2 (hpv-2), were assayed during the course of treatment. purified hpv-1 and hpv-2 were used as antigens, as well as herpes simplex virus (hsv) and concanavalin a (con a). all patients had normal percentages of subsets within the pbmc population and normal lymphoproliferative responses to con a, and those with a ... | 1992 | 1335750 |
| human papillomaviruses and skin cancer. | human papillomavirus (hpv)-induced skin warts are classically benign lesions. however an association between specific hpv types and skin cancer becomes obvious in epidermodysplasia verruciformis (ev). the analysis of this disease suggests that lesions infected with hpv types 5 and 8 carry a high risk of developing squamous cell carcinomas. the oncogenes of ev-viruses appear to be e6 and e2, rather than e7. the 'high risk' ev-viruses, hpv 5, 8, and 47, differ from related hpv types in the transfo ... | 1992 | 1335790 |
| laboratory diagnosis of latent human papillomavirus infection. | the etiologic association of human papillomavirus (hpv) with uterine cervical cancer has prompted the need for improved laboratory diagnosis of this virus. the application of conventional hybridization technology, including filter in situ hybridization (fish) and southern-blot analysis, has revealed that the detection and typing of the virus is inconsistent between sequential specimens from the same individual. to determine whether the polymerase chain reaction (pcr) can be used to provide a mor ... | 1992 | 1335864 |
| detection of human papillomavirus dna in biopsy-proven cervical squamous intraepithelial lesions in pregnant women. | this study analyzed cervical squamous intraepithelial lesions (sils) in pregnant women for human papillomavirus (hpv) using in situ hybridization analysis. hpv dna was detected in 77% (23/30) of low-grade sils as compared to 89% of such lesions in nonpregnant women. the detection rate in high-grade sils was 41% (9/22) compared to 70% in nonpregnant women. analysis by the polymerase chain reaction showed that the detection rates were similar (96-100%) for low- and high-grade lesions in pregnant a ... | 1992 | 1336051 |
| a comparison study of human papillomavirus prevalence by the polymerase chain reaction in low risk women and in a gynaecology referral group at elevated risk for cervical cancer. | the reported prevalence of human papillomavirus (hpv) type 16 in the genital tracts of women with various gynaecological conditions is highly variable. in particular, some results with the polymerase chain reaction (pcr) technique have suggested that hpv-16 is a ubiquitous or very common virus. we undertook this study to help clarify the current confusion. pcr with hpv consensus l1 primers and specific e6 primers was used to study 89 women attending two gynaecology referral clinics, as well as 9 ... | 1992 | 1336126 |
| activation of human papillomavirus type 18 e6-e7 oncogene expression by transcription factor sp1. | the human papillomavirus 18 (hpv18) e6 and e7 proteins are considered to be primarily responsive for the transforming activity of the virus. in order to analyse the molecular mechanisms resulting in viral oncoprotein expression, it is necessary to identify the factors involved in the transcriptional regulation of the e6/e7 genes. here we define by gel retardation experiments a sequence aberrant sp1 binding site present in the promoter proximal part of the viral transcriptional control region (up ... | 1992 | 1336181 |
| detection of mutations in the p53 gene in human head and neck carcinomas by single strand conformation polymorphism analysis. | using the polymerase chain reaction (pcr)-based single strand conformation polymorphism (sscp) analysis, we have examined the highly conserved regions of the p53 gene in 58 biopsy samples of head and neck tumors. mutations were found in 13/58 (23%) tumor specimens, but not in 6 normal tissues. ten of 13 mutations were due to single base changes and the remaining 3 were 1- or 8-base deletion mutants. these mutations were clustered in exons 5 and 7 and resulted in amino acid changes. our results s ... | 1992 | 1336431 |
| frequent occurrence of p53 gene mutations in uterine cancers at advanced clinical stage and with aggressive histological phenotypes. | the clinical and pathological significance of mutation of the p53 tumor-suppressor gene was examined in 108 cases of primary uterine cancers using single-strand conformation polymorphism and direct dna sequencing analyses. mutation of the p53 gene was detected in 19 (31%) of 62 cases of cancer of the uterine corpus and was more frequent in groups at an advanced clinical stage and/or with aggressive histology. among four adenocarcinomas arising in the lowest portion of the uterine corpus, three s ... | 1992 | 1336492 |
| prevalence in two mexican cities of human papillomavirus dna sequences in cervical cancer. | in mexico, about 30% of all malignant tumors in women are uterine cervix carcinomas. it is one of their main causes of death. we have previously shown that in mexico city, 31% (5/16) of the analyzed tumoral samples contained hpv-16 dna sequences. we have now extended this observation in mexico city and included the city of monterrey and found that the prevalence of hpv-16 is similar in both: 26% (6/23) for monterrey and 29% (4/14) for mexico city. hpv-18 was detected in only 10% (1/10) and 7% (1 ... | 1992 | 1336617 |
| predictive value of human papillomavirus dna detection by filter hybridization and polymerase chain reaction in women with negative results of colposcopic examination. | this study focused on women who had an abnormal papanicolaou smear and a subsequent colposcopic examination negative for a cervical squamous intraepithelial lesion. cervical swabs obtained during the initial colposcopic examination were analyzed for human papillomavirus (hpv) dna, and these results were correlated with the follow-up examinations. human papillomavirus dna was detected by dot-blot hybridization in 31 of 109 (28%) cases. the rate of subsequent biopsy-proved squamous intraepithelial ... | 1992 | 1336640 |
| human papillomavirus detection in cervical cells by in situ hybridization with biotinylated probes. | we have investigated the applicability of human papillomavirus (hpv) dna detection by in situ hybridization with biotinylated probes in epithelial cells obtained from the cervix using a cotton tip swab. we describe a simple procedure for obtaining homogeneous cell samples and good preservation of cellular structure. this is achieved by pretreatment of cells with l-cysteine before hybridization. separate denaturation of cellular dna and probe dna is also necessary for satisfactory results. both b ... | 1992 | 1336676 |
| human papillomavirus in cervix carcinoma and condylomata acuminata--identification of hpv-dna by improved dot-blot-hybridization. | using the benzoylated naphthoylated deae cellulose method (bnd-method) we have designed a more efficient approach for the detection of human papillomavirus-dna (hpv-dna) via dot-blot and hybridization. biopsy material from anogenital warts (40 patients), invasive carcinoma uteri (12 patients) and normal controls (20 patients) were studied for the presence of hpv-dna. phenol extracted dna from representative lesions was loaded onto a pretreated nitrocellulose filter, was incubated under stringent ... | 1992 | 1336719 |
| cytologic evidence of the association of different infective lesions with dysplastic changes in the uterine cervix. | cervical smears from 4055 women were examined and classified as per who criteria. 873 (21.53%) smears revealed dysplastic changes. the dysplastic smears were further examined cytologically for the presence of chlamydia trachomatis, herpes simplex virus type-2, human papilloma virus, trichomonas vaginalis and candida albicans. chlamydia was found to be the commonest micro-organism associated with cervical dysplasia followed by herpes simplex virus type-2 and human papilloma virus. the association ... | 1992 | 1336729 |
| detection of genital human papillomavirus (hpv) dna by pcr and other conventional hybridisation techniques in male partners of women with abnormal papanicolaou smears. | to study the prevalence of human papillomavirus (hpv) infection, using several different hybridisation techniques, in men whose female sexual partners had cervical hpv and/or cervical intraepithelial neoplasia (cin). | 1992 | 1336761 |
| risk factors for cervical cancer in a black elderly population: preliminary findings. | limited information is available on cervical cancer among black or elderly women. this paper presents preliminary cross-sectional data on risk factors for prevalent cervical neoplasia among 278 predominantly black, elderly women attending an inner-city, public hospital cancer-screening program. interviews were administered to obtain information on exposure variables; human papillomavirus dna hybridization was performed on 83.5% of the participants. six cases of cervical neoplasia were detected. ... | 1992 | 1337003 |
| [human papillomavirus infection of the uterine cervix in immune suppressed women after kidney transplantation]. | in 23 women, who had immunosuppressive therapy following renal transplantation, routine cytology and colposcopy were performed. colposcopically directed punch biopsies were taken from the most suspect area. if no pathologic finding was evident during colposcopy a random sample was taken from the transformation zone. histology revealed condylomatous lesions in seven cases. in two of the lesions, a cin ii was detected. to each woman with a renal allograft and therefore immunosuppressive therapy, t ... | 1992 | 1337049 |
| [the role of colposcopy in the diagnosis of hpv infection]. | human papillomavirus (hpv) has been implicated as an important etiologic factor in cervical carcinoma. this study evaluates the efficacy of colposcopy in the detection of cervical lesions with koilocytosis features. colposcopy, cervical smears and biopsy specimens from 217 women seen in our department between january-december 1991 were examined. the colposcopy examination detected 77.5% of the viral presence the histological diagnosis detected 85% of hpv, but the cytologic smears showed only 52% ... | 1992 | 1337154 |
| detection of human papillomavirus (hpv) dna type 6/11 in a conjunctival papilloma by in situ hybridization with biotinylated probes. | four cases of conjunctival papilloma in two different patients were examined by in situ hybridization for hpv dna type 6/11, 16/18 and 31/33/51. formalin-fixed and paraffin-embedded tissues were hybridized by biotinylated probes. one tumor and one of its recurrences showed nuclear positivity for hpv 6/11 in the superficial cells of the epithelium. the results suggest that hpv type 6/11 may be etiologic agent of conjunctival papillomas. the benign behavior of these neoplasms may be related to the ... | 1992 | 1337200 |
| genital human papillomavirus infection in women. | to enhance nurse clinicians' knowledge of genital human papillomavirus infection in women. | 1992 | 1337355 |
| detection of human papillomavirus dna in seborrheic keratosis by polymerase chain reaction. | we searched for hpv dna in 72 cases of seborrheic keratosis (nongenital sk 29, genital sk 43) using polymerase chain reaction. amplification of hpv dna was found in 24 (nongenital, 1; genital, 23). hpv dna type 6 was present in 18 cases and type 11 in four and type 33 in two. twenty-three (53%) cases of genital region sk and one (3%) case of nongenital region sk contained hpv dna sequences. histopathologically, the perinuclear vacuolization of epithelial cells, mitosis and dyskeratotic cells wer ... | 1992 | 1337468 |
| the absence of genital human papillomavirus dna in virginal women. | our aim was to determine the prevalence of human papillomavirus (hpv) infection in virginal women and to establish if other forms of sexual activity not involving penetration may contribute to the transmission of this infection. female patients attending medical practitioners, and female high school students were recruited. each participant answered an anonymous self answer questionnaire and collected a tampon specimen. tampons were analysed for the presence of human papillomavirus by the polyme ... | 1992 | 1337472 |
| detection of human papilloma virus types 16 & 18 dna in cervical lesions of indian women using in situ hybridization. | fifty one biopsies from women with malignant lesions of the uterine cervix and 9 biopsies fron non-malignant lesions were examined for the presence of hpv 16 and 18 dna sequences by in situ hybridization method using 35s-labelled dna probes. hpv 16 dna sequences were detected in 82.4 per cent biopsies from women with malignant lesions, whereas hpv 18 dna was detected in only 3 biopsies which were also positive for hpv 16 dna. two biopsies from non-malignant lesions were positive for hpv 16 dna o ... | 1992 | 1337748 |
| detection of human papillomavirus dna in invasive cervical cancers by the polymerase chain reaction and its clinical significance. | in order to detect human papillomavirus (hpv) dna in invasive cervical cancers, three different polymerase chain reactions to amplify different subgenomic fragments of hpv dna were carried out on dna extracted from 93 formalin-fixed and paraffin-embedded tumor tissues. this study detected hpv dna in 54 cases (58.1%), which broke down to hpv 16 in 39 (41.9%) cases, hpv 18 in six (6.4%), hpv 52 in three, hpv 33 in one and unclassified hpv type in the remainder. histopathologically, squamous cell c ... | 1992 | 1337817 |
| abnormal cervical cytology in adolescents: a literature review. | this article reviews the literature on the subject of cervical cytological abnormalities in teenagers, defined as a papanicolaou (pap) smear result more severe than inflammation. there is discussion of the increasing prevalence of this problem in adolescents. behavioral and biologic risk factors are examined. the role of the human papillomavirus, widely believed to be the etiologic agent, is addressed. atypia, its relationship to cervical malignancies, and its management are reviewed. the possib ... | 1992 | 1337840 |
| [human papillomavirus type 16 and human cytomegalovirus in cervical carcinoma]. | using techniques of dna-dna dot blot hybridization, we examined 98 cervical specimens for the presence of human papillomavirus type 16 hpv-16 and human cytomegalovirus (hcmv) sequence and analysed the relationship between virus infection and various cervical diseases, age of patients, age at first coitus, age of first child birth, the number of pregnancy, use of oral contraceptives. the results showed that the rate of hpv dna and hcmv dna positivity in cervical carcinoma is significantly higher ... | 1992 | 1337876 |
| occurrence and expression of human papillomavirus type 16 genes in uterine cervical carcinomas. | the detection of human papillomavirus (hpv) type 16 early genes: e7, e5, and the late gene: l1 was attempted in 42 uterine cervical neoplasia (35 cervical carcinomas and 7 cervical dysplasias) using the polymerase chain reaction (pcr) method. consequently, e7 gene was detected in 19 (54.3%) of 35 carcinomas and in 5 (71.4%) of 7 dysplasias, e5 gene was detected in 7 (20.0%) of 35 carcinomas and in 5 (71.4%) of 7 dysplasias, l1 gene was detected in 18 (51.4%) of 35 carcinomas and in 5 (71.4%) of ... | 1992 | 1338065 |
| epidermodysplasia verruciformis. | epidermodysplasia verruciformis is a rare skin disease characterized by the disseminated, flat, wart-like lesions caused by human papillomavirus and a high frequency of various skin cancers. the clinical aspects, histological findings, genetic and immunological factors, and human papillomavirus types found in the disease and their roles in skin cancers are reviewed. | 1992 | 1338070 |