| radiation-induced dna damage and lethality in e. coli as modified by the antitumor agent cis-dichlorodiammineplatinum (ii). | | 1977 | 331388 |
| resistance of germfree rats to indomethacin-induced intestinal lesions. | indomethacin given orally to conventional rats produced in three days a syndrome, often fatal, of intestinal lesions characterized by multiple ulcers and peritonitis. male germfree rats were found to be resistant to this effect of indomethacin, while female germfree rats developed very mild lesions. germfree rats became sensitive again to such lesions when monocontaminated with e. coli. in such animals, however, the lesions were less severe than in conventional animals, presumably because more t ... | 1977 | 331401 |
| bacteremia after renal transplantation. | of 291 patients who received renal allotransplants in 1964-74, 94 patients developed 121 bacteremic episodes within the first 6 months after transplantation. the death rate was 38%. the bacteremic episodes occurred mainly during the first 2-3 months after transplantation. in 65% of the episodes bacteremia was secondary to urinary tract infections. the bacteria fourn were most often gram-negative rods such as escherichia coli, klebsiella and proteus. predisposing factors were ureteral complicatio ... | 1977 | 331458 |
| a microbiological study of gastro-enteritis in black infants. | a survey was carried out to determine the aetiological agents responsible for acute gastro-enteritis in black infants. bacteriological examination of 191 patients and 178 age-matched controls showed that salmonellae and shigellae together accounted for only 15% of cases and were harboured by almost 7% of controls. seropositive 'enteropathogenic' escherichia coli was an important cause of diarrhoea, especially in the summer months. enterotoxin-producing e. coli was not a significant cause of gast ... | 1977 | 331509 |
| [vaginitis in the bitch (author's transl)]. | during a period of two and a half years (2 1/2), sixty-two patients wre submitted to the department of veterinary obstetrics and gynaecology, where a diagnosis of vaginitis was established. these patients often were young large dogs. most of the females showed a vaginal discharge, although some bitches were only very attrative to males. there were no complaints concerning the general physical condition of the patients. on clinical examination, the vulva was found to be swollen. exudation was als ... | 1977 | 331556 |
| [resistance to phage ms2 induced in e. coli by infection with that phage]. | a sensitive cell of e. coli ab 259 hfr 3000 infected with rna-containing phage ms2 produces phages and simultaneously continues to divide showing a segregation of sensitive cells which maintain new cycles of the infection. phage multiplication in the sensitive cell induces phage-resistant forms in the progeny of this cell. the described phenomenon is not caused by selection of pre-existing f--cells, but may result from a direct interaction of phage products with the episomal dna coding proteins ... | 1977 | 331608 |
| lower urinary tract pathogens in the dog and their sensitivity to chemotherapeutic agents. | ninety-six urine samples were collected by a sterile technique from 75 dogs affected with urinary tract disease (cystitis, urolithiasis, prostatitis, etc) involving bacteruria. the infecting organisms were isolated and tested against sensitivity discs (penicillin g, streptomycin, chloramphenicol, tetracycline, sulphamethoxazole/trimethaprin and sulphatriad). the commonest isolate was escherichia coli, which was generally sensitive to several agents, though in eight cases it was resistant to all ... | 1977 | 331657 |
| [studies of immunity in mastitis in cows]. | studies were carried out to establish the specific antibodies in the milk and blood sera of cows recovered from a spontaneous infection of the udder caused by streptococci, staphylocci, corynebacteria, and some representatives of escherichia coli as well as of cows immunized with a polybacterial vaccine. followed up was likewise the resistance of the udder in vaccinated cows to bacterial infections. it was found that in both groups of animals the specific antibodies' titer ranged from 1:80 to 1: ... | 1977 | 331658 |
| circular dimers of a lambda dna in infected, nonlysogenic escherichia coli. | covalently closed circular dimers of phage lambda dna have been found in escherichia coli infected with lambda. these dimers can be formed by either the lambda red or int systems, by a nonrecombinational replicative mechanism requiring the activity of the lambda o and p genes or by joining of the cohesive ends. dimers mediated by the e. coli rec system have not been observed. those formed by the int system often result from recombination between different dna molecules; however, the red-mediated ... | 1977 | 331660 |
| restriction of bacteriophage p2 by the escherichia coli ri plasmid, and in vitro cleavage of its dna by the ecori endonuclease. | | 1977 | 331662 |
| in vitro synergy of cefamandole-tobramycin combinations. | | 1977 | 331694 |
| [demonstration of escherichia coli antigens and antibodies using the erythrocyte migration inhibition test]. | | 1977 | 331712 |
| mercury resistance and r plasmids in clinical isolates of kebsiella pneumoniae. | the mercury and antibiotic resistance of 155 clinical isolates of klebsiella pneumoniae (k. pneumoniae) was determined. resistance to mercury tested was found in 64.5% of the total isolates. the frequencies of resistance to sm, tc, cp, km and gm were 65.8%, 49.0%, 48.4%, 37.4% and 3.9%, respectively. and we selected 94 mercury and drug resistant strains, and they were tested for their ability to transfer the resistance to the mercury and drug sensitive recipients of e. coli k12 ml 1410-nx and e. ... | 1977 | 331756 |
| development and use of single "polytropic" diagnostic tubes for the approximate taxonomic grouping of bacteria isolated from foods, water and medicinal preparations. | single tubes, containing in all instances a bottom layer of a solid medium for detecting mode of attack on glucose, lactose, mannitol or starch and top layers of solid, semi-solid or liquid media allowing assessment of motility, formation of catalase, oxidase, coagulase, indole, hydrogen sulphide, acetyl methyl carbinol or pigments, as required ("polytropic" diagnostic tubes) were developed for the approximate taxonomic grouping of bacteria commonly encountered in foods, water and medicinal prep ... | 1977 | 331757 |
| [study of the role of the plasmid-determined sucrose index in salmonella newport in the development of experimental infectious process]. | the authors studied the role played by sac-plasmide of atypical s. newport strains inducing a more severe course of the disease in the development of the infectious process in man. in experiments with genetically-bound strains of salmonellae and e. coli k12, differing by the presence of sac-plasmide, it was shown that the acquisition or the loss of the latter failed to effect significantly the capacity of the microbes to cause death of chick embryos infected on the membrane, to reproduce in the ... | 1977 | 331763 |
| [clinico-laboratory substantiation of possible use of the indirect hemagglutination test for the interpretation of diagnosis of enteropathogenic e. coli carrier state]. | the diagnostic value of the indirect hemagglutination (iha) test in deciphering the diagnosis of enteropathogenic e. coli carrier state was found in this work; this was done by comparing the specific antibody level in carriers and patients suffering from coli-infection and also by establishing the correlation between the results of the iha test and the clinical and laboratory examination of carriers. | 1977 | 331766 |
| [group diseases induced by enteropathogenic escherichia coli (epec) serotype 018 among newborns]. | | 1977 | 331780 |
| [phage conversion of escherichia and shigella antigens. i. phage conversion of type i antigen of sh. flexneri in entrophatogenic e. coli o129]. | the authors realized conversion of type i sh. flexneri in enteropathogenic e. coli o129 with converting moderate phage phi i sh. flexneri. phage phi i lysogenized 7.3--42.7% of the cells of antigenic e. coli variant o129 which lost type v antigen; conversion of the type i antigen of sh. flexneri was revealed in the agglutination and adsorption of agglutinins tests. as a result, e. coli strain was obtained with the o-antigen identical to the o-antigen of sh. flexneri ia. | 1977 | 331793 |
| cellular and humoral factors involvement in the enhanced nbt reduction by neutrophil leucocytes of newborn infants. | the histochemical nbt test was performed on blood samples from ten healthy newborn infants. high spontaneous nbt reduction has been confirmed for neutrophils and assessed for monocytes. the stimulation of both neutrophils and monocytes with escherichia coli endotoxin induces a statistically significant increase of nbt positive cells. the reason for the false positive test results in neonates was investigated by incubating neutrophils from adult donors and for different time periods in either neo ... | 1977 | 331852 |
| origin of intestinal beta-glucuronidase in germfree, monocontaminated and conventional rats. | the intestinal beta-glucoronidase was studied in germfree, monocontaminated and conventional rats. the greater part of the beta-glucuronidase of the caecum and the large intestine of the contaminated animals was of bacterial origin. no bacterial beta-glucuronidase was found in the small intestine. monocontamination with escherichia coli gave activities corresponding to those of the conventional rats, whereas content from the caecum and the large intestine of the rats monocontaminated with strept ... | 1977 | 331863 |
| a critique of oral therapy of dehydration due to diarrheal syndromes. | under the circumstances of limited health resources and immediate need for preventing the dehydration associated with diarrhea in infants, breastfeeding should be encouraged throughout the diarrheal episode. when this is not possible because of cessation or failure of lactation, an oral electrolyte solution should be administered. it should be sterile and provide a quantity of electrolytes not greatly in excess of 30 meq/liter of sodium and potassium. there should be little possibility of an ... | 1977 | 331934 |
| potentiometric study by amalgam electrodes of the interaction between escherichia coli trna and divalent copper, zinc, or cadmium ion in an aqueous neutral buffer solution. | | 1977 | 332008 |
| [study of the raffinose character in "escherichia coli" and "salmonella" (author's transl)]. | | 1977 | 332028 |
| susceptibility to kasugamycin of escherichia coli carrying conjugative and nonconjugative r plasmids. | ten conjugative and two nonconjugative r plasmids, all of which were naturally isolated, were tested for their ability to confer epistatic susceptibility to kasugamycin upon the host escherichia coli. all of the conjugative plasmids, which belonged to various incompatibility groups, conferred a plasmid-determined epistatic susceptibility to kasugamycin upon the host cells, whereas the nonconjugative plasmids did not. | 1977 | 332069 |
| bactericidal activity of the combinations of gentamicin with clindamycin or chloramphenicol against species of escherichia coli and bacteroides fragilis. | the bactericidal activity of clindamycin, chloramphenicol, and gentamicin alone and of gentamicin plus clindamycin and gentamicin plus chloramphenicol was studied on 8 strains of escherichia coli and 10 strains of bacteroides fragilis isolated from clinical material. gentamicin did not interfere with the activity of clindamycin or chloramphenicol against b. fragilis. the activity of gentamicin against e. coli was not influenced by clindamycin, but chloramphenicol suppressed the rapid bactericida ... | 1977 | 332070 |
| spread of a single plasmid clone to an untreated individual from a person receiving prolonged tetracycline therapy. | the aerobic gram-negative intestinal flora of two individuals living in close proximity was followed for 17 months. one of these persons was receiving a prolonged tetracycline treatment for acne vulgaris and was colonized by tetracycline-resistant escherichia coli strains throughout the survey. the other person studied received no antibiotics during the period in question, but was frequently found to excrete a number of e. coli strains harboring tetracycline-resistant plasmids. the two e. coli s ... | 1977 | 332073 |
| substrate interactions with the alpha-subunit of the escherichia coli tryptophan synthase. a kinetic study of the wild-type alpha-subunit. | | 1977 | 332076 |
| substrate interactions with the alpha-subunit of the escherichia coli tryptophan synthase. a study of the activity of mutant alpha-subunits. | | 1977 | 332077 |
| reaction of pyridoxal 5'-phosphate with escherichia coli coa transferase: evidence for an essential lysine residue. | | 1977 | 332079 |
| sulfhydryl group reactivity in the escherichia coli coa transferase. | | 1977 | 332080 |
| substrate-induced conformational changes and half-the-sites reactivity in the escherichia coli coa transferase. | | 1977 | 332081 |
| a simple procedure for resolution of escherichia coli rna polymerase holoenzyme from core polymerase. | | 1977 | 332084 |
| purification and characterization of protein synthesis initiation factors if1, if2, and if3 from escherichia coli. | | 1977 | 332085 |
| [experimental studies on the pathogenesis of coli-enterotoxemia in swine. 4. effect of lipopolysaccharide endotoxin on weaned piglets following parenteral administration]. | ten clinically intact weaned piglets were experimentally intoxicated by intravenous injection of lipoproteide-free lipopolysaccharide endotoxin according to westphal of e. coli o 127:b8. severe endotoxin shock with all clinical manifestations of experimental coli-enterotoxaemia was induced in all animals and included circulatory disorder with tachycardia, intermittent pallor and/or cyanosis, symptoms of severe systemic intoxication, neurological symptoms, such as lack of coordination, hindleg st ... | 1977 | 332109 |
| [quantitative analysis of the gastrointestinal flora in the piglet prior and after weaning with special reference to the pathogenesis of coli-enterotoxemia]. | the gastro-intestinal flora of clinically intact piglets on an industrialised sow unit was investigated prior to weaning (n = 12) and after weaning (n = 12). the age of the former group was 23 to 36 days and that of the latter between six and eleven days. while enormous proliferation of haemolysing e. coli was recorded from the anterior portion of the small intestine in the post-weaning group, weaning, quite, generally, was found to have only little impact upon the gastrointestinal flora of pigl ... | 1977 | 332111 |
| effect of dimethyl sulphoxide on the expression of nitrogen fixation in bacteria. | storage in dimethyl sulphoxide (dmso) of escherichia coli k12 hybrids carrying nif+ genes from klebsiella pneumoniae can result in selection of a defective nitrogen-fixing phenotype. similar results are obtained with e. coli k12 hybrids containing the nitrogen-fixing capacity from rhizobium trifolii. dmso appears to affect particular inner membrane proteins associated with energy metabolism in e. coli k12 and four chromosomal regions (chld, chlg, his and unc) are associated with resistance to dm ... | 1977 | 332135 |
| [enterotoxin-producing e.-coli bacteria in enteritis and edema disease of swine. quick diagnosis of heat-labile and heat resistant toxin]. | | 1977 | 332152 |
| [comparative serological studies on escherichia coli strains with b and l antigens from swine and calf. 2. on immunogenity of common l antigens in cultures prepared at 18 degrees c]. | | 1977 | 332155 |
| a temperature-sensitive mutant of escherichia coli with an altered rna polymerase beta' subunit. | | 1977 | 332156 |
| n-trimethylalanine, a novel blocking group, found in e. coli ribosomal protein l11. | | 1977 | 332162 |
| adjustment of rna content during temperature upshift in escherichia coli. | | 1977 | 332163 |
| acetylation stoichiometry of escherichia coli pyruvate dehydrogenase complex. | | 1977 | 332166 |
| precursors of major outer membrane proteins of escherichia coli. | | 1977 | 332169 |
| effect of metal cations on misincorporation by e. coli dna polymerases. | | 1977 | 332171 |
| a temperature sensitive protein in outer membrane of escherichia coli k-12 harbouring a temperature sensitive r plasmid, rts1. | | 1977 | 332173 |
| apurinic acid endonuclease implicated in dna breakage in escherichia coli subjected to mild heat. | | 1977 | 332174 |
| accumulation of tetracyclines by escherichia coli k-12. | | 1977 | 332179 |
| structure-function relationship in escherichia coli initiation factors. i. phosphorylation of if-3 by coliphage t7 protein kinase. | | 1977 | 332180 |
| febrile response and variations in the brain levels of biogenic amines and their metabolites in rabbits made tolerant to e. coli lipopolysaccharide. | | 1977 | 332181 |
| functional lac carrier proteins in cytoplasmic membrane vesicles isolated from escherichia coli. 1. temperature dependence of dansyl galactoside binding and beta-galactoside transport. | | 1977 | 332221 |
| functional lac carrier proteins in cytoplasmic membrane vesicles isolated from escherichia coli. 2. experimental evidence for a segregation of the lac carrier proteins induced by a conformational transition of the membrane lipids. | | 1977 | 332222 |
| structural requirements for aminoacylation of escherichia coli formylmethionine transfer rna. | | 1977 | 332227 |
| phospholipid exchange between bilayer membranes. | the mode of interaction of aqueous dispersions of phospholipid vesicles is investigated. the vesicles (average diameter 950 a) are prepared from total lipid extracts of escherichia coli composed of phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. one type of vesicle contains trans-delta 9-octadecenoate, the other type trans-delta 9-hexadecenoate as predominant acyl chain component. the vesicles show order in equilibrium disorder transitions at transition temperatures, tt = 42 degr ... | 1977 | 332230 |
| kinetics of phospholipid exchange between bilayer membranes. | in an accompanying publication by duckwitz-peterlein, eilenberger and overath ((1977) biochim. biophys. acta 469,311--325) it is shown that the exchange of lipid molecules between negatively charged vesicles consisting of total phospholipid extracts from escherichia coli occurs by the transfer of single lipid monomers or small micelles through the water. here a kinetic interpretation is presented in terms of a rate constant, k--, for the escape of lipid molecules from the vesicle bilayer into th ... | 1977 | 332231 |
| [interaction between a fluorescent probe and the surface structures of escherichia coli]. | the interaction of ans with escherichia coli cells, isolated cell walls, total cell envelopes and inner membranes was investigated in the presence and absence of ca2+ ions. the addition of ca2+ to intact cells at room temperature did not result in enhancement of fluorescence intensity. on the contrary the addition of ca2+ to intact cells at 2 degrees c resulted in a progressive increase of fluorescence intensity with the maximum reached approximately by the 20th minute. the addition of ca2+ to d ... | 1977 | 332234 |
| [the influence of chloramphenicol on synthesis of ribosomes and beta and beta' subunits of rna polymerase]. | the effect of low chloramphenicol concentrations on the biosynthesis of rna, ribosomal proteins and rna polymerase in e. coli cp 78 cells was studied. when protein synthesis was decreased by 50--70%, 14c-uracil incorporation in dna increased twice, the rrna synthesis being stimulated preferentially. in the presence of antibiotic the rna/dna ratio increased from 5,7 to 13,3. the differential rate of r-protein synthesis increased simultaneously with the stimulation of rrna synthesis, so that alpha ... | 1977 | 332237 |
| evolutionary significance of the system utilizing d-amino acid enantiomers. | the evolutionary significance of the system utilizing d-amino acid enantiomers in living organisms is discussed, based on an experiment in which the mutant from escherichia coli k--12 4627 grown on d-tryptophan was used. the mutant shows the ability of d-tryptophan is degraded to indol which can be utilized for the synthesis of l-tryptophan in the presence of serine. | 1977 | 332246 |
| [behavior of the f-like plasmids in bacterial cells with a varying capacity genetic recombination]. | f-like plasmids (fbl, fbl drd, f-lac+) were transferred to the recipient strains e. coli, k-12 with different recombination ability. then the sensitivity of these strains to ultra-violet irradiation and also the stability of the mentioned plasmids under conditions of bacteria treatment with elimination agents was determined. the presence of any f-like plasmids under study failed to influence the ultra-violet sensitivity of the bacterial cells. on the other hand, the research results of spontaneo ... | 1977 | 332251 |
| intestinal emphysema (pneumatosis cystoides intestinalis) in a gnotobiotic pig. | a gnotobiotic pig monocontaminated with an enteropathogenic escherichia coli was subsequently hyperimmunized to produce a monotypic antiserum. at necropsy, multiple, air filled cysts were found in the wall of the large intestine. the etiology of this condition is still conjectural. however, select strains of e. coli may cause or contribute to intestinal emphysema in swine. | 1977 | 332291 |
| effect of heat stable and heat labile escherichia coli enterotoxins, cholera toxin and theophylline on unidirectional sodium and chloride fluxes in the proximal and distal jejunum of weanling swine. | acute, isolated loops of proximal and distal jejunum of weanling swine were exposed to either heat stable porcine escherichia coli enterotoxin, heat labile porcine escherichia coli enterotoxin, cholera toxin or theophylline. unidirectional sodium fluxes in response to heat stable in the proximal jejunum were dependent on the length of time that the intestinal mucosae was exposed to the enterotoxin. net water, sodium and chloride and unidirectional sodium and chloride flux measurements in the pro ... | 1977 | 332292 |
| phage typing of escherichia coli isolated from chickens. | ten different bacteriophages were isolated from untreated city sewage water. these phages were stable at 57 degrees c for 40 min. a modified agar layer technique was used to obtain high titre phages. ninety-four of a stock of 101 cultures of escherichia coli, which were isolated from inflamed portions of intestines of chickens, were lysed by one or more of these phages. the e. coli of a known serological grouping were phage typed. | 1977 | 332294 |
| flow-microfluorometric analysis of escherichia coli, rhizobium meliloti, and rhizobium japonicum at different stages of the growth cycle. | the applicability of flow-microfluorometry (fmf) to the study of bacterial samples was investigated on cultures of rhizobium meliloti, rhizobium japonicum, and escherichia coli using fluorescent and light-scattering signals. this technique which analyzes individual bacterial cells in a population was used to monitor the relative change in nucleic acid content and cell size during the growth cycle of the three microorganisms which were known to have different growth rates. early log-phase e. coli ... | 1977 | 332295 |
| structural studies of the o-specific side-chains of the cell-wall lipopolysaccharide from escherichia coli o 69. | | 1977 | 332362 |
| cyclacillin-induced potentiation of escherichia coli immunogenicity in vivo and in vitro. | the efficacy of cyclacillin as an antimicrobial agent against escherichia coli was assessed in vivo in mice infected with low numbers of bacteria and compared to the relative effectiveness of the antibiotic against the same organisms in vitro. treatment of mice with cyclacillin resulted in a rapid clearance of e. coli from the blood and their greater killing in the spleen and liver. furthermore, a significantly higher antibody plaque response against e. coli developed in cyclacillin-treated mic ... | 1977 | 332464 |
| effects of arsenite on dna repair in escherichia coli. | since environmental exposure to arsenicals has been correlated with a high skin cancer risk among populations exposed to sunlight, it is possible that arsenicals might interfere with the repair of damage to dna (mostly thymine dimers) resulting from the ultraviolet rays in sunlight. to test this hypothesis, strains of e. coli, differing from each other only in one or more repair functions, were exposed to uv light and then plated in the presence or absence of sodium arsenite. survival after irra ... | 1977 | 332497 |
| a topographical study of the 5'-region of 16 s rna of escherichia coli in the presence and absence of protein s4. | | 1977 | 332520 |
| further characterisation of the rna structure in the binding region of protein s4 on 16 s ribosomal rna of escherichia coli. | | 1977 | 332521 |
| hydrodynamic properties of protein s1 from escherichia coli ribosome. | | 1977 | 332522 |
| predictions for secondary structures of six proteins from the 50 s subunit of the escherichia coli ribosome. | | 1977 | 332523 |
| primary structure of protein l28 from the large subunit of escherichia coli ribosomes. | | 1977 | 332524 |
| comparison of the n-terminal sequences of aspartate and ornithine carbamoyltransferases of escherichia coli. | | 1977 | 332525 |
| the stability of nascent ribosomal ribonucleic acid in growing escherichia coli. | | 1977 | 332550 |
| [discovery of a new type of restriction and modification in a group of intestinal bacteria]. | the adsorption of 23 new lambdoid bacteriophages to 547 strains which isolated from natural population of enterobacteriaceae was studied. the frequency of positive combinations of phage-bacterium with adsorption is not more than 2%. a study of possible causes of limited growth of lambdoid phages in the bacterial strains revealed that neither homoimmune prophage nor prophage p2 are single factors of the growth limitation. it is found that in natural populations a selection of bacterial strains wi ... | 1977 | 332589 |
| production of colicin v in vitro and in vivo and observations on its effects in experimental animals. | in recent years, a possible relationship between pathogenicity and colicinogeny in some escherichia coli strains responsible for gastrointestinal infection and bacteremia in man and animals has been inferred. using enterotoxigen-negative, colicin v-producing e. coli strains, we have (i) elaborated a simple in vitro method for producing greater yields of colicin v free of bacterial cells and large, non-dialyzable molecules; (ii) detected the presence of the bacteriocin in peritoneal fluids of mor ... | 1977 | 332624 |
| microtiter solid-phase radioimmunoassay for detection of escherichia coli heat-labile enterotoxin. | the development of a microtiter solid-phase radioimmunoassay for the detection of vibrio cholerae enterotoxin and heat-labile escherichia coli enterotoxin is described. the test is based on the immunological similarity between v. cholerae toxin and e. coli heat-labile toxin. the assay is easy to perform, quantitative, and at least as sensitive and specific as the y-1 adrenal cell system. | 1977 | 332626 |
| immunological interrelationships between cholera toxin and the heat-labile and heat-stable enterotoxins of coliform bacteria. | cholera toxin (ct) and the heat-labile (lt) toxin of escherichia coli are known to share antigenic properties. the present study examined the immunological relationship of ct and the lt and heat-stable (st) toxins of e. coli, klebsiella pneumoniae, and enterobacter cloacae. the neutralizing capacity of equine ct antiserum and of antiserum raised in rabbits to the lt toxin of the three species of coliform bacteria was evaluated by determining their capacity to inhibit the action of purified ct an ... | 1977 | 332637 |
| role of bacterial phospholipases in serum-mediated killing of escherichia coli. | the importance of bacterial phospholipases during serum-mediated killing of escherichia coli was examined by using wild-type dr+ ds+ and an isogenic phospholipase-deficient mutant dr- ds-. no difference in serum sensitivity was observed when the parental dr+ ds+ and mutant dr- ds- strains were exposed to various concentrations of normal guinea pig serum. examination of the free fatty acid (ffa) and lipid composition during serum-mediated killing of the two e. coli strains indicated that ffa rele ... | 1977 | 332640 |
| effect of some unicellular algae on escherichia coli populations in sea water and oysters. | | 1977 | 332678 |
| role of atp in removal of psoralen cross-links from dna of escherichia coli permeabilized by treatment with toluene. | removal of interstrand cross-linked from dna was examined in escherichia coli permeabilized by treatment with toluene. under these conditions, the reaction requires atp and mg2+, and the mechanism appears to be similar to that occurring in whole cells. under optimum conditions, the rate constant was 0.06 min-1. genetical, physical, and biochemical analysis of the repair process suggest the following mechanism. in an atp-dependent reaction, the uvra and uvrb gene products cleave a phosphodiester ... | 1977 | 332687 |
| identification and organization of ribosomal protein genes of escherichia coli carried by lambdafus2 transducing phage. | we describe the isolation of lambdafus2, which carries bacterial dna from the str-spc region of the escherichia coli chromosome. genes for 27 ribosomal proteins were found on the genome of this transducing phage by identifying the ribosomal proteins whose synthesis was stimulated after infection of uv-irradiated bacteria. these were genes for s3, s4, s5, s7, s8, s10, s11, s12, s13, s14, s17, s19, l2, l3, l4, l5, l6, l14, l15, l16, l17, l18, l22 l23, l24, l29, and l30. subsets of these genes were ... | 1977 | 332690 |
| organization of ribosomal protein genes of escherichia coli as analyzed by polar insertion mutations. | several mutants of lambdaspc1 and lambdafus3 have been isolated carrying dna insertion elements that were selected for their ability to reduce the expression of the spc gene. the sizes and locations of the insertions on the phage genomes were determined by heteroduplex analysis. they were found to be located at different positions in the spc transcription unit. the effect of these insertions on the expression of the ribosomal protein genes carried by these phages in ultraviolet light-irradiated ... | 1977 | 332691 |
| insertion of dna carrying ribosomal protein genes of escherichia coli into charon vector phages. | dna fragments from lambdaspc1 and lambdafus2, carrying ribosomal protein genes from escherichia coli, were inserted into lambda phage vectors charon 3 and charon 4. eight of the resulting clones were characterized by agarose gel electrophoresis of ecori digests, analytical cscl equilibrium centrifugation, and electron micrographic analysis of heteroduplexes. in each case, the identity, order, and orientation of each cloned fragment was determined. in all, 8 of the 12 ecori fragments of lambdafus ... | 1977 | 332692 |
| development of specific tests for rapid detection of escherichia coli and all species of proteus in urine. | proteus mirabilis was distinguishable from escherichia coli and from several other species that may be associated with urinary tract infections when grown in a nutrient medium supplemented with 0.1 m l-methionine by the formation of large amounts of dimethyl disulfide and methyl mercaptan, which were detected by head-space gas-liquid chromatography (hs-glc). e. coli could be detected by the same hs-glc technique by ethanol production from methionine peptone water enriched with 1% either lactose ... | 1977 | 332706 |
| assessment of technique for rapid detection of escherichia coli and proteus species in urine by head-space gas-liquid chromatography. | a test depending on the production of ethanol by escherichia coli from lactose and dimethyl disulfide by proteus spp. from methionine in the early exponential phase of growth and the detection of these products by head-space gas-liquid chromatography has been applied to 75 specimens of urine selected to provide the most stringent trial of the test. the test was found to be rapid and reliable for the commonest findings in the microbiological examination of urine. in 3 to 4 h it detected "signific ... | 1977 | 332707 |
| superoxide dismutase in bacteroides fragilis and related bacteroides species. | superoxide dismutase (sod) activity was demonstrated in cell-free extracts of bacteroides fragilis, bacteroides vulgatus, bacteroides distasonis, bacteroides ovatus, and bacteroides thetaiotaomicron. the strains were grown under anaerobic conditions in trypticase soy broth, and the specific activity of sod in the extracts was, in most strains, higher than in cell-free extracts of escherichia coli b grown under anaerobic conditions. isoelectric focusing of the extracts in polyacrylamide gel demon ... | 1977 | 332713 |
| rapid screening method for enterotoxigenic escherichia coli. | a rapid screening method for detection of escherichia coli producing heat-labile enterotoxin is described. single colonies are transferred directly from primary culture plates into 96-well microculture plates containing 0.3 ml of brain heart infusion broth in each well. after 24 h at 37 degrees c, each brain heart infusion broth culture is assayed by the miniculture method in the corresponding well of a microculture plate in which y-1 mouse adrenal tumor cells have been grown. all enterotoxigeni ... | 1977 | 332715 |
| iodinated e. coli 70s ribosomes as a radiocolloid of uniform particle size for lymph-node and liver scanning. | as an example of the use of biologic particles as carriers for radioactive tracers, e. coli 70s ribosomes were labeled with i-125 using chloramine-t. the labeled ribosomes, after treatment with glutaraldehyde, were injected into rabbits either subcutaneously (through the dorsum of the foot) or intravenously (through the ear). after subcutaneous injection, 40% of the activity accumulated in the lymph nodes during the first 5 hr, and the 70s ribosomal particles were shown to remain within the lymp ... | 1977 | 332744 |
| in vivo transfer of an r factor within the lower gastro-intestinal tract of sheep. | the transfer of an r factor from donor e. coli introduced into the rumen of adult sheep to strains of the coliform microflora resident post rumen in the lower gastro-intestinal tract was found to be greatly increased when the animals were subjected to a short period of starvation (ca. 24-48 h). this also resulted in coliform organisms containing the resistance determinants of the r factor being excreted for much longer periods, sometimes for months afterwards. as no antibiotic treatment was give ... | 1977 | 333023 |
| comparison of escherichia coli from bacteriuric patients with those from feces of healthy schoolchildren. | the properties of 709 strains of escherichia coli isolated from feces of healthy schoolchildren were compared with those of 115 strains from the urine of girls with asymptomatic bacteriuria (abu) detected in a screening program. these fecal strains were also compared with 45 strains that caused asymptomatic reinfections and 10 that caused symptomatic reinfections in the same group of girls. typing of o antigen was done by direct bacterial agglutination, and k typing was done with a serum agar te ... | 1977 | 333035 |
| studies with a new generation of oral attenuated shigella vaccine: escherichia coli bearing surface antigens of shigella flexneri. | in an attempt to develop a safe, proliferating, oral, attenuated vaccine against shigellosis, genes that control the synthesis of group- and type-specific somatic antigens of shigella flexneri 2a were transferred via conjugation to a recipient strain of escherichia coli. the resultant hybrid (e. coli expressing shigella surface antigens) vaccine strain, pgai 42-1-15, believed to have a complete (smooth) lipopolysaccharide, was given to volunteers in two vaccination-challenge studies. the vaccine ... | 1977 | 333041 |
| transfer of multiple antibiotic resistance from bacteroides fragilis to escherichia coli. | multiple antibiotic resistance was transferred from a clinical isolate of bacteroides fragilis to a strain of escherichia coli k12. resistance to ampicillin, amoxicillin, cephalothin, tetracycline, minocycline, and chloramphenicol was transferred as a unit, but the resistance markers became segregated during storage of the recipient strains. | 1977 | 333042 |
| evidence for a centrally mediated hypotensive effect of escherichia coli endotoxin in the anesthetized dog. | the centrally mediated cardiovascular effects of escherichia coli endotoxin were studied utilizing a neurally intact vascularly isolated head-trunk preparation in the anesthetized dog. the vascularly isolated head was perfused at constant flow with arterial blood supplied by a donor animal. spectrophotometric examination of the donor and recipient trunk blood after administration of evan's blue dye indicated that there was no blood exchanged between the head and trunk of the recipient dog. the r ... | 1977 | 333096 |
| overlapping transcriptional units in the deo operon of escherichia coli k-12. evidence from phage mu-1 insertion mutants. | | 1977 | 333116 |
| isolation, characterization and structural implications of a nuclease-digested complex of aminoacyl transfer rna and escherichia coli elongation factor tu. | | 1977 | 333117 |
| inactivation of receptors for bacteriophage t5 during infection of escherichia coli b. | during infection of escherichia coli by bacteriophage t5, the cell surface receptors for the phage were inactivated so that they could not be isolated from the infected cells. a mutant of t5 that could only inject 8% of the t5 dna did not cause the inactivation. | 1977 | 333128 |
| chemical relaxation kinetic studies of e. coli rna polymerase binding to poly [d(a--t)] using ethidium bromide as a fluorescence probe. | | 1977 | 333270 |
| [the periurethral flora in female patients with recurrent urinary tract infection (uti) (author's transl)]. | in 52 girsl and 38 women with recurrent nonobstructive uti 529 bacterial samples were obtained from the periurethral area under the following conditions: no bacteriuria and no chemotherapy for at least 3 weeks before the examination. in 225 samples we found e. coli and enterococci as the most frequent potentially uropathogenic bacteria. in the periurethral region these were found approximately twice as frequently as in urologically normal persons. 36 (40%) of the patients had no further recurren ... | 1977 | 333275 |
| the influence of prophage lambda on the mutagenic response of escherichia coli to ultraviolet irradiation. | e. coli k-12 and its lambda-lysogenic derivative were used to study the influence of lysogenic induction on the induction of mutants (streptomycin sensitivity to streptomycin resistance) by uv irradiation. the lambda-lysogenic strain responded to uv irradiation with increased sensitivity and with a strong reduction of the absolute mutant quantity, recovered per uv dose, as compared with the non-lysogenic parental strain. however, similar mutant freqqencies per surviving bacterium exclude the pos ... | 1977 | 333278 |
| frameshift mutagenesis in bacteria by 8-methoxypsoralen (methoxalen) in the dark. | we confirm that 8-methoxypsoralen (8-mop) in the dark induces frameshift mutations in both escherichia coli and salmonella typhimurium when present in adequate concentration under growth conditions. the dose response is sigmoidal with a threshold or quasi-threshold at concentrations below about 10 microgram/ml. frameshift mutagenesis by 8-mop in the dark is unaffected by mutations at the uvra or uvrb genes, in contrast to base pair substitution mutagenesis by 8-mop plus near uv light. reca (but ... | 1977 | 333279 |
| productive t7 infection of escherichia coli f+ cells and anucleate minicells. | | 1977 | 333295 |