| membranolytic activity of actinobacillus actinomycetemcomitans leukotoxin. | | 1991 | 1831848 |
| actinobacillus actinomycetemcomitans leukotoxin is a calcium-binding protein. | | 1991 | 1831850 |
| microbial risk indicators for periodontal attachment loss. | | 1991 | 1831856 |
| bacteriological study of juvenile periodontitis in china. | the predominant cultivable bacteria associated with juvenile periodontitis (jp) in china were studied for the first time. subgingival plaque samples were taken on paper points from 23 diseased sites in 15 jp patients and from 7 healthy sites in 7 control subjects. serially diluted plaque samples were plated on nonselective blood agar and on mgb agar, a selective medium for the isolation of actinobacillus actinomycetemcomitans. fifteen or more isolated colonies from each sample (in sequence witho ... | 1991 | 1832453 |
| multicenter evaluation of tetracycline fiber therapy. iii. microbiological response. | in a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ... | 1991 | 1832457 |
| tetracycline fiber therapy monitored by dna probe and cultural methods. | oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ... | 1991 | 1832458 |
| cell attachment activity of cementum proteins and mechanism of endotoxin inhibition. | cementum occupies a unique anatomical location where soft connective tissues of the periodontium are attached to root surfaces. cell attachment properties of proteins present in cementum were studied. human and bovine cementum were extracted with 0.5 mol/l ch3cooh followed by 4 mol/l guanidine, and proteins were separated by ion-exchange chromatography and sds-polyacrylamide gel electrophoresis. cells were labeled with radioactive amino acids and added to tissue-culture plastic plates incubated ... | 1991 | 1833426 |
| the regulatory effects of monocytes on human natural killer cells activated by lipopolysaccharides. | lipopolysaccharides (lps) rapidly enhance cytotoxicity of human natural killer (nk) cells against tumor targets. the regulatory effects of peripheral blood monocytes (mo) on this activation were measured. when lymphocytes were kept at a constant number in culture containing lps from oral and enteric bacteria, increasing the percentage of mo caused a dose-dependent suppression of nk cytotoxicity. this suppression was reversed by adding the prostaglandin (pg) inhibitor indomethacin which indicates ... | 1991 | 1837053 |
| use of synthetic oligonucleotide dna probes for the identification of different strains of fusobacterium nucleatum. | the ability of three different oligonucleotide probes to identify different oral strains of fusobacterium nucleatum was tested. probes 119 and 214 were designed on the basis of known amino acid sequences from the n-terminal end of an outer membrane protein of f. nucleatum strain fev1. probe h 2.1, was a randomly cloned chromosomal dna fragment of 2.1 kbp. the specificities of the probes were examined by testing each of them against a panel of five other species of fusobacterium (six strains), or ... | 1991 | 1837056 |
| analysis of actinobacillus pleuropneumoniae and related organisms by dna-dna hybridization and restriction endonuclease fingerprinting. | the objective of this study was to determine the degree of genetic relatedness of actinobacillus pleuropneumoniae to selected members of the family pasteurellaceae, with particular emphasis on species commonly associated with swine. free-solution dna-dna hybridization studies revealed that representative strains of all 12 serotypes of a. pleuropneumoniae formed a homogeneous group, sharing 74 to 90% sequence homology with a. pleuropneumoniae serotype 1. all serotypes of a. pleuropneumoniae teste ... | 1991 | 1847295 |
| synergistic effects between amoxicillin, metronidazole, and the hydroxymetabolite of metronidazole against actinobacillus actinomycetemcomitans. | interactions between metronidazole and amoxicillin, metronidazole and its hydroxymetabolite, and amoxicillin and the hydroxymetabolite of metronidazole were investigated with checkerboard titrations in combination with accurately determined mics and mbcs. actinobacillus actinomycetemcomitans was used as the test organism. synergism was found for all three combinations. fractional inhibitory concentration indices and fractional bactericidal concentration indices varied from 0.3 to 0.7. these syne ... | 1991 | 1854177 |
| evidence for invasion of a human oral cell line by actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans, an oral bacterial species associated with periodontal disease, was found to invade human cell lines. invasion was demonstrated by recovery of viable organisms from gentamicin-treated kb cell monolayers and by light and electron microscopy. internalization occurred through a cytochalasin d-sensitive process. invasion efficiencies of some a. actinomycetemcomitans strains were comparable to those of invasive members of the family enterobacteriaceae. differences ... | 1991 | 1855989 |
| assessing periodontal disease activity. the role of bacteriological, immunological, and dna assays. | | 1991 | 1856752 |
| the actinobacillus pleuropneumoniae hemolysin determinant: unlinked appca and appbd loci flanked by pseudogenes. | the appbd genes encoding the secretion functions for the 110-kda rtx hemolysin of actinobacillus pleuropneumoniae have been cloned and sequenced. unlike analogous genes from other rtx determinants, the appbd genes do not lie immediately downstream from the hemolysin structural gene, appa. although isolated from a diverse group of gram-negative organisms, the appbd genes and the characterized rtx bd genes from other organisms all exhibit a high degree of homology at both the dna and predicted ami ... | 1991 | 1860823 |
| construction of a dna probe and detection of actinobacillus pleuropneumoniae by using polymerase chain reaction. | a 1.5-kb actinobacillus pleuropneumoniae 4074 dna fragment from a genomic library was found to hybridization. no cross-hybridization hybridization. no cross-hybridization was detected with dnas from hemolytic members of the family pasteurellaceae. from the nucleotide sequence of the putative genomic probe, three primers were synthesized for use in polymerase chain reactions (pcrs), with 31 strains tested by using purified and crude dna targets. pcr amplification products of 610 and 985 bp were o ... | 1991 | 1864937 |
| incidence of periodontitis recurrence in treated patients with and without cultivable actinobacillus actinomycetemcomitans, prevotella intermedia, and porphyromonas gingivalis: a prospective study. | a total of 98 adults previously treated for moderate to advanced periodontitis and on a trimonthly recall schedule were screened for the presence of critical levels of actinobacillus actinomycetemcomitans, prevotella (bacteroides) intermedia, and porphyromonas (bacteroides) gingivalis. patients with at least 2 positive sites were placed in a positive group and patients without or with low levels of these bacteria in a negative group. during the 30-month study the incidence of disease recurrence ... | 1991 | 1870068 |
| application of quadferm+ for the identification of fastidious gram-positive and gram-negative bacilli. | the 2-hr quadferm+ kit (qf) (analytab products, plainview, ny) was compared with conventional tube tests for the identification of the hacek bacteria (haemophilus aphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, kingella kingae), other haemophilus and kingella spp., capnocytophaga, corynbacterium, and moraxella spp. test results were identical for 296 comparisons with 74 isolates (74%). in the remaining 104 comparisons for 26 isolates, 50 reactions ... | 1991 | 1873978 |
| actinobacillus pleuropneumoniae-induced thymic lesions in mice and pigs. | actinobacillus pleuropneumoniae produces several hemolysins/cytotoxins that may be important in the pathogenesis of acute lesions. little is known, however, about the role of these virulence factors in chronic disease or the carrier state. we investigated the effects of live bacterial infection and transthoracic injection of a sterile culture supernatant on primary lymphoid organs and lymphocyte populations. transthoracic inoculation of mice or intranasal inoculation of pigs with virulent a. ple ... | 1991 | 1879915 |
| in vitro killing of actinobacillus actinomycetemcomitans and capnocytophaga spp. by human neutrophil cathepsin g and elastase. | the purpose of this study was to compare the killing of actinobacillus actinomycetemcomitans with that of capnocytophaga spp. by purified cathepsin g and elastase in vitro. both were sensitive to killing by purified cathepsin g, but only the capnocytophaga spp. were killed by elastase. killing by cathepsin g exhibited logarithmic kinetics, was enhanced slightly by alkaline ph, and was enhanced greatly under hypotonic conditions. treatment of cathepsin g with diisopropyl fluorophosphate significa ... | 1991 | 1879926 |
| nucleotide sequence of the hemolysin i gene from actinobacillus pleuropneumoniae. | the dna sequence of the gene encoding the structural protein of hemolysin i (hlyi) of actinobacillus pleuropneumoniae serotype 1 strain 4074 was analyzed. the nucleotide sequence shows a 3,072-bp reading frame encoding a protein of 1,023 amino acids with a calculated molecular size of 110.1 kda. this corresponds to the hlyi protein, which has an apparent molecular size on sodium dodecyl sulfate gels of 105 kda. the structure of the protein derived from the dna sequence shows three hydrophobic re ... | 1991 | 1879928 |
| identification of hemolytic and cytotoxic proteins of actinobacillus pleuropneumoniae by use of monoclonal antibodies. | monoclonal antibodies (mabs) were raised against extracellular hemolytic and cytotoxic proteins of actinobacillus pleuropneumoniae serotypes 2 and 9. mabs were tested for inhibition of hemolytic and cytotoxic activities of the reference strains of a. pleuropneumoniae serotypes 1 to 12. five mabs inhibited hemolytic or cytotoxic activities of one or more serotypes and were used to probe western blots (immunoblots) of cytotoxic and hemolytic culture filtrates of serotypes 1 to 12. the mabs recogni ... | 1991 | 1879932 |
| lethal effects of actinobacillus actinomycetemcomitans leukotoxin on human t lymphocytes. | the majority of strains of actinobacillus actinomycetemcomitans isolated from patients with periodontal diseases secrete a leukotoxin that destroys human myeloid cells within minutes but has no effect on viability of peripheral blood lymphocytes in culture for 1.5 h. however, since this organism persists in the gingival crevice and thus may continuously release toxin over extended periods of time, we assessed the viability of t cells cultured with leukotoxin (0 to 250 ng/ml) for up to 2 days. al ... | 1991 | 1879940 |
| cross-reactivity between actinobacillus pleuropneumoniae serotypes comparing different antigens and serological tests. | several antigens were prepared from suspensions of reference strains of the 12 serotypes of actinobacillus pleuropneumoniae (saline extract [se], capsular extract [ce], whole cell suspension [wcs], boiled extract [be], and autoclaved cell antigen [aca]). the cross reactions between each antigen and the antisera against reference strains of the other 11 serotypes were compared by using the complement fixation test, elisa and the indirect haemagglutination test. aca produced the most cross reactio ... | 1991 | 1882138 |
| biochemical typing of actinobacillus pleuropneumoniae. | a study was conducted to evaluate the possibility of using biochemical differences among strains of a given serotype of actinobacillus pleuropneumoniae as epidemiological markers, to rapidly identify the source of infection in herds affected with swine pleuropneumonia. out of 38 different biochemical and physiological tests performed on a total of 67 strains belonging to serotypes 1 and 5 of a. pleuropneumoniae, three fermentation tests, glycerol, lactose and raffinose, allowed the classificatio ... | 1991 | 1882510 |
| a model for demonstrating the adhesion of actinobacillus seminis to epithelial cells. | the objective of this study was to demonstrate that a field isolate of actinobacillus seminis (as8c) will adhere to epithelial cells and that this adhesion can be inhibited by pretreating the bacteria with mouse serum containing polyclonal antibodies (poabs) prepared against this isolate. an indirect fluorescent antibody test, transmission electron microscopy, and phase-contrast microscopy confirmed the adhesion of as8c to an established culture of bovine kidney epithelial cells (bkecs). in a ba ... | 1991 | 1884292 |
| the influence of disease on feed and water consumption and on pharmacokinetics of orally administered oxytetracycline in pigs. | in the present study the feed and water consumption and pharmacokinetic parameters of orally administered oxytetracycline were compared in clinically healthy pigs and in the same pigs following a challenge with actinobacillus (haemophilus) pleuropneumoniae toxins. endobronchial challenge with a. pleuropneumniae toxins was accompanied by anorexia, increased lassitude, labored breathing, fever, and increased white blood cell counts. pleuropneumonia was evident in all pigs on autopsy. following the ... | 1991 | 1885404 |
| 6-deoxy-d-talan and 6-deoxy-l-talan. novel serotype-specific polysaccharide antigens from actinobacillus actinomycetemcomitans. | serotype-specific polysaccharide antigens from actinobacillus actinomycetemcomitans atcc 29523 (serotype a) and nctc 9710 (serotype c) were extracted from whole cells by autoclaving and purified by ion-exchange chromatography and gel filtration. analysis of component sugars by gas-liquid chromatography-mass spectrometry, high performance liquid chromatography, and nmr together with optical rotation data showed that the serotype a antigen was composed solely of 6-deoxy-d-talose, whereas the serot ... | 1991 | 1885566 |
| immunological properties of actinobacillus pleuropneumoniae hemolysin i. | the 105 kda hemolysin i protein from actinobacillus pleuropneumoniae serotype i type strain 4074 (hlyi) was shown by immunoblot analysis to be the predominant immunogenic protein if convalescent field sera or sera from pigs experimentally infected with a. pleuropneumoniae serotype 1 were used. sds gel- and immunoblot-analysis using total culture, washed cells or culture supernatant showed that hlyi is essentially secreted and is not found attached to the bacteria. proteins in the 105 kda range t ... | 1991 | 1887566 |
| localized juvenile periodontitis: a case report. | | 1991 | 1889386 |
| blocking enzyme-linked immunosorbent assay for detection of antibodies to actinobacillus pleuropneumoniae serotype 2. | a blocking enzyme-linked immunosorbent assay (elisa), based upon a polyclonal rabbit antiserum specific to actinobacillus pleuropneumoniae serotype 2, was developed for the detection of antibodies to a. pleuropneumoniae serotype 2 in pigs. by testing sera from pigs experimentally infected with the 11 recognized serotypes of a. pleuropneumoniae, the assay was proven to be specific for a. pleuropneumoniae serotype 2. with field sera from herds infected with a. pleuropneumoniae serotype 2, the assa ... | 1991 | 1890179 |
| the use of genomic dna fingerprinting in studies of the epidemiology of bacteria in periodontitis. | recent studies of microbial epidemiology emphasizing the genetic organization and distribution of organisms associated with orofacial infections have led to new insights into the possible origins of pathogenicity. studies into genetic heterogeneity, acquisition and transmission of these organisms have been markedly advanced by the utilization of the powerful technique of genomic dna fingerprinting. characteristic fingerprints for each bacterial isolate can be produced by cleavage of high molecul ... | 1991 | 1890219 |
| microbiology in the management of destructive periodontal disease. | this paper summarizes the rationale for the application of microbiology in the management of destructive periodontal diseases. the subgingival microbiota in patients with severe periodontitis is complex and contains high numbers of obligate anaerobic bacteria as well as facultative micro-organisms. it has become clear that major differences exist in the composition of the subgingival microflora. these differences are not only quantitative but also qualitative. difference in plaque composition is ... | 1991 | 1890220 |
| dental bacterial plaques. nature and role in periodontal disease. | antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ... | 1991 | 1890226 |
| differential killing of actinobacillus actinomycetemcomitans and capnocytophaga spp. by human neutrophil granule components. | the purpose of this study was to determine whether granule fractions of human neutrophils differentially kill actinobacillus actinomycetemcomitans and capnocytophaga spp. granule extracts were subjected to gel filtration, and seven fractions (designated a through g) were obtained. under aerobic conditions at ph 7.0, representative strains of a. actinomycetemcomitans were killed by fraction d and variably by fraction b. in contrast, the capnocytophaga spp. were killed by fractions c, d, f, and g. ... | 1991 | 1894375 |
| characterization of a non-hemolytic mutant of actinobacillus pleuropneumoniae serotype 5: role of the 110 kilodalton hemolysin in virulence and immunoprotection. | to determine the role of hemolysin(s) in virulence and immunoprotection, non-hemolytic mutants of actinobacillus pleuropneumoniae serotype 5, strain j45, were isolated following chemical mutagenesis. one mutant was selected for extensive characterization. differences in capsule content, or in lipopolysaccharide or membrane protein electrophoretic profiles of the parent and mutant were not detected. a predominant, calcium-inducible protein of 110 kda was present in culture supernatant of the pare ... | 1991 | 1895928 |
| identification and partial characterization of the hemolysin (hlyii) of actinobacillus pleuropneumoniae serotype 2. | the secreted hemolytic activity produced by actinobacillus pleuropneumoniae serotype 2 reference strain is thermolabile, inactivated by proteinase k and requires ca2+ as cofactor for its hemolytic activity. purification of the hemolytic activity resulted in a fraction containing two proteins, one of 105 kda and one of 125 kda. these two proteins could be further separated by preparative sds polyacrylamide gel electrophoresis. this purification step, resulted in loss of the hemolytic activity. po ... | 1991 | 1897133 |
| distribution of protein d, an immunoglobulin d-binding protein, in haemophilus strains. | protein d, a novel surface protein of the bacterial species haemophilus influenzae with specific affinity for human immunoglobulin (ig) d was detected in all 127 h. influenzae strains studied. all strains representing different serotypes of encapsulated strains and different biotypes of nonencapsulated strains bound 125i-labeled igd to a high degree (38 to 74%). sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot (immunoblot) analysis showed that protein d from all h. infl ... | 1991 | 1900807 |
| microbial aspects of failing osseointegrated dental implants in humans. | the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ... | 1991 | 1906537 |
| cross-reactivity and antigenic heterogeneity among actinobacillus pleuropneumoniae strains of serotypes 4 and 7. | actinobacillus pleuropneumoniae strains of serotypes 4 and 7 were studied for their antigenic properties by means of agglutination, coagglutination, indirect hemagglutination, immunodiffusion, and counterimmunoelectrophoresis tests. strains of serotype 4 showed cross-reactivity with those of serotype 7 in various serological tests. serotype 7 strains were antigenically heterogeneous and shared common antigens with several other serotypes. by using boiled whole-cell saline extract as the antigen ... | 1991 | 1909343 |
| factors in virulence expression and their role in periodontal disease pathogenesis. | the classic progression of the development of periodontitis with its associated formation of an inflammatory lesion is characterized by a highly reproducible microbiological progression of a gram-positive microbiota to a highly pathogenic gram-negative one. while this gram-negative microbiota is estimated to consist of at least 300 different microbial species, it appears to consist of a very limited number of microbial species that are involved in the destruction of periodontal diseases. among t ... | 1991 | 1912148 |
| bacteriologic monitoring of periodontal diseases: cultural, enzymatic, immunologic, and nucleic acid studies. | evidence supporting the bacteriologic etiology of periodontal diseases has led investigators to monitor the microbiologic species associated with each disease. the type of study performed has evolved at different levels over the years and is predominantly influenced by available technologies. as the technology has advanced, so too have attempts at monitoring specific microorganisms. the ultimate goal in most research efforts on clinical bacteriologic monitoring of the periodontal diseases has be ... | 1991 | 1912631 |
| calcium binds to and is required for biological activity of the 104-kilodalton hemolysin produced by actinobacillus pleuropneumoniae serotype 1. | actinobacillus pleuropneumoniae serotype 1, strain shope 4074, was grown on agar medium containing 10 mm ca2+ and under ca2+ limiting conditions by addition of 2 and 5 mm egta to the growth medium. hemolysis of washed bovine erythrocytes was observed from the culture grown in ca2+ excess but not from the two cultures where ca2+ was chelated from the growth medium by using egta. however, the hemolytic activity of these latter two cultures could be restored if 10 mm ca2+ was added to the dilution ... | 1991 | 1913343 |
| investigation of the structure of lipid a from actinobacillus actinomycetemcomitans strain y4 and human clinical isolate po 1021-7. | the lipopolysaccharides of actinobacillus actinomycetemcomitans strain y4 and a human clinical isolate po 1021-7 were examined by sds/page, deoxycholate/page and mass spectrometry. page analysis revealed an electrophoretic pattern similar to the sr-type lipopolysaccharide (lps) of salmonella. deoxycholate/page indicated the lps of a. actinomycetemcomitans to consist of short sugar chains. chemical analysis revealed the presence of thiobarbituric-acid-positive material (3-deoxy-d-manno-octulosoni ... | 1991 | 1915349 |
| [comparison of some subgingival microflora from juvenile periodontitis before and after scaling and root planing]. | actinobacillus actinomycetemcomitans and capnocytophaga are subgingival bacteria that have correlation with juvenile periodontitis. studies were carried out in a group of 20 patients with juvenile periodontitis before, after treatment by scaling, root planing, oral hygiene instruction and 20 patients with healthy gingiva as a control group. gingival index, plaque index, pocket depth were measured. subgingival bacteria were counted on selective media and compared by phase contrast microscopy. bef ... | 1991 | 1918569 |
| isolation and purification of bacteriocin from prevotella intermedia (bacteroides intermedius). | thirty-three out of 46 strains of prevotella intermedia (bacteroides intermedius) isolated from gingival deposits of patients with adult periodontitis possessed the ability to inhibit growth of p. intermedia strain atcc 25611, and 6 of the 33 showed strong inhibitory activity. thirteen isolates did not have the ability to inhibit the growth of p. intermedia strain atcc 25611. an inhibition factor from one of the isolates, p. intermedia th14 which had strong inhibition, was purified and character ... | 1991 | 1920011 |
| effect of giving enrofloxacin in the diet to pigs experimentally infected with actinobacillus pleuropneumoniae. | three groups of 16 pigs were exposed individually when four weeks old to intranasal infection with 10(8.9) viable actinobacillus pleuropneumoniae (serovar 3, strain 2/(10p2); a fourth group was kept in isolation from the others as uninfected controls. seven days later the 62 surviving animals were killed and necropsied. the organism had caused typical, mainly subacute disease in 12 of the 16 unmedicated animals but in only two of the 16 which had had continuous access to a diet containing 150ppm ... | 1991 | 1926685 |
| actinobacillus suis-like organisms and evidence of hemolytic strains of actinobacillus lignieresii in horses. | thirty-seven local isolates of actinobacillus suis-like organisms from diseased and clinically normal horses and 1 llama were compared with reference strains of a suis, a lignieresii, a equuli, a capsulatus, a hominis, a (pasteurella) ureae, and equine a suis-like organisms (aslo) previously described in literature. comparison was by cultural characteristics, carbohydrate fermentation, enzyme profiles, and whole-cell protein polyacrylamide gel electrophoresis. carbohydrate fermentation, determin ... | 1991 | 1928905 |
| molecular distribution of rob-1 beta-lactamase in actinobacillus pleuropneumoniae. | the plasmid content and molecular distribution of the rob-1 beta-lactamase was investigated in 31 swine isolates of actinobacillus pleuropneumoniae. three types of plasmid patterns were observed in ampicillin-resistant isolates: a predominant one consisting of 2.6-, 2.9-, and 5.0-kb plasmids and two other patterns consisting of 4.2- and 5.5-kb plasmids and of a 6.8-kb plasmid. plasmid dna preparations were hybridized with a blarob-1 intragenic fragment from the haemophilus influenzae rrob plasmi ... | 1991 | 1929299 |
| crossed immunoelectrophoresis applied to representative strains from 11 different pasteurella species under taxonomic aspects. | crossed immunoelectrophoresis evaluated on a numerical basis revealed a close antigenic relationship between species of the genus pasteurella. by cluster analysis, 4 groups on similarity levels between 87% and 72% s could be separated which were connected by a minimum level of 69.5% s. one subgroup included all biovars or subspecies consisting of strains with mucoid growth. another feature governing the antigenic relationship seemed to be the host range of pasteurella species. despite a consider ... | 1991 | 1930562 |
| some unusual members of the family pasteurellaceae isolated from human sources--phenotypic features and genomic relationships. | twelve human isolates exhibiting the properties of the family pasteurellaceae but phenotypically deviating from established species, or resembling species of animal origin that are only rarely reported to occur in human materials, were checked for their identities by dna-dna hybridization. the collection consisted of one strain of actinobacillus lignieresii, two strains of actinobacillus hominis (mannose-positive), two hitherto undescribed actinobacillus or actinobacillus-like species, [pasteure ... | 1991 | 1930571 |
| subgingival microbiota in squirrel monkeys with naturally occurring periodontal diseases. | the squirrel monkey (saimiri sciureus) has been proposed as an in vivo model for the study of subgingival colonization by suspected periodontopathogens, such as black-pigmented porphyromonads and prevotellas (bp/p). however, the indigenous microbiota of the squirrel monkey has not been well described. therefore, in order to more fully characterize the oral microbiota of these animals, we studied two groups of squirrel monkeys from widely different sources. group i consisted of 50 breeding colony ... | 1991 | 1937762 |
| isolation and molecular characterization of spontaneously occurring cytolysin-negative mutants of actinobacillus pleuropneumoniae serotype 7. | actinobacillus pleuropneumoniae serotype 7 strains are shown to spontaneously lose cytolytic activity with a frequency of approximately 10(-4). the phenotypic change is associated with the loss of approximately 8.5 kbp of chromosomal dna. a genomic fragment encoding the cytolysin and its flanking sequences was cloned and characterized. also, the corresponding truncated fragment was cloned from a spontaneous mutant. comparison of the two clones allowed the definition of the excision site. the end ... | 1991 | 1937770 |
| cytolysins of actinobacillus pleuropneumoniae serotype 9. | cytolysin i (clyi) and cytolysin ii (clyii), which are present in the culture supernatant of actinobacillus pleuropneumoniae serotype 9, are thought to play an important role in the pathogenesis of pig pleuropneumonia. the purpose of this study was to clone and characterize the genetic determinants of these cytolysins. cloning was accomplished by the screening of dna libraries for the presence of cytolytic activity and for the presence of dna sequences homologous to leukotoxin dna of pasteurella ... | 1991 | 1937809 |
| nuclease-sensitive binding of an actinobacillus actinomycetemcomitans leukotoxin to the bacterial cell surface. | a leukotoxin of actinobacillus actinomycetemcomitans 301-b was solubilized from cell-associated membrane vesicles by treatment with externally added dnase and rnase and was further purified by a procedure which included ammonium sulfate fractionation, gel filtration chromatography, and ion-exchange chromatography. the purified toxin had a molecular mass of 113,000 da by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and a high isoelectric point (approximately 8.8). from these characte ... | 1991 | 1937819 |
| transformation of actinobacillus actinomycetemcomitans by electroporation, utilizing constructed shuttle plasmids. | actinobacillus actinomycetemcomitans, a periodontal pathogen, has been strongly implicated in human periodontal disease. advances in the molecular analysis of a. actinomycetemcomitans virulence factors have been limited due to the unavailability of systems for genetic transfer, transposon mutagenesis, and gene complementation. slow progress can be traced almost exclusively to the lack of gene vector systems and methods for the introduction of dna into a. actinomycetemcomitans. an electrotransfor ... | 1991 | 1937823 |
| detection and management of the high risk periodontal patient. | periodontal diseases and patients at risk for progression of disease can be diagnosed on the basis of age, previous episodes of periodontal disease and loss of attachment, as detected by periodontal probing and radiographic evaluation. for early onset periodontitis, family history may be important. microbiological evaluations are excellent determinants of disease and also appear to be probable predictors of 'at risk' patients. reliable tests, including chairside tests, are available for the thre ... | 1991 | 1937850 |
| [the transmission of actinobacillus actinomycetemcomitans between spouses]. | the aim of this investigation was to study the transmission of actinobacillus actinomycetemcomitans (a.a) between husband and wife when one of them was affected by acute juvenile periodontitis (p.a.j.). the hypothesis tested was the role played by the reservoir of the non-infected partner in explaining the frequent recurrences of the disease in the affected spouse despite combined antibiotherapeutical and surgical treatments. twenty subjects with p.a.j. were studied. a.a. was found in the sub-gi ... | 1991 | 1939039 |
| [the distribution and prevalence of haemophilus actinomycetemcomitans in the oral cavity]. | the prevalence and distribution of haemophilus actinomycetemcomitans (h.a.) were studied in 3292 specimens of subgingival plaque on the four subgingival aspects of all teeth of the dental arch, 150 specimens from the mucosal surface (tongue and cheek) and 30 saliva specimens in 30 subjects. the sample population of 30 subjects was subdivided into three groups: 10 normal subjects, 10 subjects with localised juvenile periodontitis (sljp) and 10 subjects with adult chronic periodontitis (sacp). the ... | 1991 | 1939044 |
| early changes in cytosolic calcium and membrane potential induced by actinobacillus actinomycetemcomitans leukotoxin in susceptible and resistant target cells. | actinobacillus actinomycetemcomitans produces a cytolytic peptide leukotoxin which kills susceptible target cells, including human neutrophils, monocytes, lymphocytes, and hl-60 promyelocytic leukemia cells. cell death occurs as a consequence of colloid osmotic lysis. in the present investigation early leukotoxin-induced changes in membrane permeability were studied by flow cytometry and quantitative spectrofluorimetry in leukotoxin-susceptible and resistant targets. within 5 s toxin-susceptible ... | 1991 | 1940358 |
| analysis of in vitro lymphoproliferative responses and antibody formation following subcutaneous injection of actinobacillus actinomycetemcomitans and wolinella recta in a murine model. | the potential of wolinella recta and actinobacillus actinomycetemcomitans to cause abscesses and induce an immune response was tested in balb/c mice. mice were injected subcutaneously with w. recta, a. actinomycetemcomitans or a mixture of these 2 microorganisms. mice injected with a. actinomycetemcomitans alone, or with both organisms, demonstrated abscesses at the injection site 2 days later, from which pure cultures of a. actinomycetemcomitans were isolated. mice injected with w. recta had sm ... | 1991 | 1945478 |
| dna probe detection of periodontal pathogens in hiv-associated periodontal lesions. | recent studies have shown that an atypical gingivitis and a rapidly progressive periodontal disease may be early-occurring opportunistic infections associated with human immunodeficiency virus (hiv) infection. this study examined the prevalence of selected periodontal pathogens associated with these hiv-related periodontal lesions. subgingival plaque samples were obtained from both hiv-seronegative and hiv-seropositive homosexual men and from presumably uninfected heterosexual men. dna probes we ... | 1991 | 1945481 |
| distribution of actinobacillus actinomycetemcomitans serotypes in periodontal health and disease. | a total of 177 actinobacillus actinomycetemcomitans isolates from 136 periodontally healthy or diseased subjects were serotyped by indirect immunofluorescence and/or immunodiffusion assays. serotype-specific rabbit antisera against a. actinomycetemcomitans serotypes a, b and c were used. all 3 serotypes were commonly found in the study subjects. serotype b was dominant in subjects with periodontal disease and serotype c was the most common serotype in the healthy subjects. in the immunofluoresce ... | 1991 | 1945486 |
| are certain oral pathogens part of normal oral flora in denture-wearing edentulous subjects? | a complete loss of teeth may eliminate some pathogenic bacteria for lack of a suitable habitat for colonization. the occurrence of actinobacillus actinomycetemcomitans, black-pigmented bacteroides, mutans streptococci, lactobacilli and yeasts was investigated in 51 edentulous subjects with complete denture(s); mean age 74 years (range 44-92 years). none of the subjects had received antibiotics during the preceding 6 months. four swab samples per subject were taken from the fitting surface of the ... | 1991 | 1945487 |
| dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque. | this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ... | 1991 | 1945492 |
| studies on the possibility of rearing seronegative animals within a breeding and rearing herd infected with actinobacillus pleuropneumoniae serotype 2. | in two sanitation trials it was not possible to rear seronegative animals within a breeding and rearing herd heavily infected with actinobacillus pleuropneumoniae serotype 2. neither medication with sulphonamides-trimethoprim of the sows and piglets or the sows alone nor a strictly applied all-in all-out system reduced the percentage of seropositive animals. | 1991 | 1949544 |
| immunological characterization of breakdown peptides of the 104 kilodalton hemolysin of actinobacillus pleuropneumoniae serotype 1. | the 104 kilodalton (kda) hemolysin of actinobacillus pleuropneumoniae serotype 1, strain cm-5 was precipitated from rpmi-1640 culture supernatant using ammonium sulfate to 80% saturation. in immunoblots, a rabbit polyclonal antiserum against the 104 kda hemolysin protein, recognized not only the original 104 kda monomeric form of the hemolysin but other proteins in the crude antigen mixture ranging in molecular mass from 43 to greater than 125 kda. the antiserum was able to crosslink these prote ... | 1991 | 1949556 |
| an abattoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. i. prevalence and morphological description of gross lung lesions. | the prevalence and pathomorphology of gross lung lesions were studied in 855 slaughter weight pigs from 9 selected herds in south-eastern norway. pneumonic or pleuritic lesions were found in 84% of the lungs, ranging from 37% in the least affected herd to 97% in the one most affected. bronchopneumonia indicative of a primary mycoplasma hyopneumoniae infection was found in 70% of the lungs, ranging from 9% to 82% in the individual herds. the amount of bronchopneumonic lesions in individual lungs ... | 1991 | 1950852 |
| an abattoir survey of pneumonia and pleuritis in slaughter weight swine from 9 selected herds. iii. serological findings and their relationship to pathomorphological and microbiological findings. | blood samples from 777 pigs, originating from 9 different herds, were collected at slaughter and examined for antibodies to mycoplasma hyopneumoniae and actinobacillus (haemophilus) pleuropneumoniae by the indirect hemagglutination assay (iha) and the complement fixation (cf) test, respectively. results were compared to pathological and microbiological findings. antibodies to m. hyopneumoniae in positive titers of 1/80 or higher were found in 62% of the samples. the relationship between positive ... | 1991 | 1950854 |
| structural studies of the capsular polysaccharide from actinobacillus pleuropneumoniae serotype 12. | the capsular polysaccharide of a. pleuropneumoniae is composed of 2-acetamido-2-deoxy-d-glucose (3 parts) and phosphate (1 part). the arrangement of these components in the repeating unit was determined by dephosphorylation, methylation, g.l.c.-m.x., and 1d and 2d n.m.r. spectroscopic methods. the polysaccharide was found to be a high molecular weight polymer of repeating trisaccharide units, joined through phosphate diester linkages, having the structure, (formula: see text). | 1991 | 1959118 |
| structure and function of the b and d genes of the actinobacillus actinomycetemcomitans leukotoxin complex. | the actinobacillus actinomycetemcomitans leukotoxin gene complex, consisting of four genes, has been cloned and the sequence of the aaltc and aalta genes reported. the present paper details the sequences of the aaltb and aaltd genes which, like aaltc and aalta, are also homologues of genes found in other cytolytic toxin complexes of several other gram-negative bacterial pathogens. when tested in a recombinant expression system, the aaltb and/or aaltd genes are required for the translocation and ... | 1991 | 1961107 |
| molecular analysis of periodontal pathogens. | both bacteroides gingivalis and actinobacillus actinomycetemcomitans have been implicated in the destruction of periodontal tissues. to understand better the role putative virulence factors from the two bacterial species may play in an infection, the collagenase gene from bact. gingivalis and the leucotoxin gene from a. actinomycetemcomitans were cloned. as it is intended to generate strains carrying defined mutations in these genes for in vitro and in vivo experiments, extensive restriction map ... | 1990 | 1965114 |
| [identification and typing of actinobacillus actinomycetemcomitans by restriction endonuclease analysis]. | the aim of the present study was to investigate the potential of restriction endonuclease analysis (rea) for the identifying and typing of actinobacillus actinomycetemcomitans (aa). bacterial chromosomal dna was extracted and digested with bamhi, hindiii, sal i and xho i respectively and separated by agarose gel electrophoresis. dna fragment patterns of aa and haemophilus aphrophilus differed strikingly from each ether. the patterns of aa fdc y4 and aa atcc 29523, belonging to two different sero ... | 1990 | 1965593 |
| drug-susceptibility and isolation of a plasmid in haemophilus (actinobacillus) pleuropneumoniae. | we isolated 56 haemophilus (actinobacillus) pleuropneumoniae strains from the pneumonic porcine lung tissues and tested them for antimicrobial susceptibility. two drug-resistant strains were obtained. one, named kh-265, was resistant to streptomycin (sm) and sulfonamide (sa), and the other, named kh-195, was resistant to tetracycline (tc). the minimum inhibitory concentrations (mics) of drugs for resistant strains were 100 micrograms/mliters for sm, 3200 micrograms/mliters for sa, and 12.5 micro ... | 1990 | 1968988 |
| colonial variation and fimbriation of actinobacillus actinomycetemcomitans. | three colonial variants of actinobacillus actinomycetemcomitans, which formed transparent rough (tr)-, transparent smooth (ts)-, and opaque smooth (os)-surfaced colonies, were described in relation to their fimbriation. tr- and ts-cells were adhesive to agar and glass surfaces but not the os-cells. the examination by electron microscopy revealed that tr-cells were highly fimbriated but not ts- and os-cells. thus, ts-cells seemed to be an intermediate type. the fimbriae were isolated from tr-cell ... | 1990 | 1974223 |
| [a membrane-bound alanine aminopeptidase from acinetobacter calcoaceticus. 3. inhibition of the enzyme]. | the alanine aminopeptidase from acinetobacter calcoaceticus is inhibited by sh-reagents like p-hydroxymercuribenzote, ellman's reagent, n-bromosuccinimide, and metal chelating agents like 1,10-phenanthroline. the aap is competitively inhibited by l-amino acids such as leucine, phenylalanine, and valine having hydrophobic side chains. bacitracin (ki = 2.0.10(-6) mol/l) inhibits aap stronger than puromycin (ki = 8.0.10(-6) mol/l). in contrast, the aeromonas aminopeptidase (ec 3.4.11.10) is stronge ... | 1990 | 1980190 |
| genetic approach to the study of epidemiology and pathogenesis of actinobacillus actinomycetemcomitans in localized juvenile periodontitis. | actinobacillus actinomycetemcomitans isolates from periodontal pockets were examined for restriction fragment-length polymorphism using a characterized 4.7-kb dna probe. a total of 6 patterns of rflp was found in 133 isolates originating from 12 subjects. no relatedness was found between rflp types and serotypes. different periodontal sites within the same subject and different individuals within the same family sometimes showed only one type of a. actinomycetemcomitans rflp. when members among ... | 1990 | 1982406 |
| murine macrophage interleukin-1 release by capsularlike serotype-specific polysaccharide antigens of actinobacillus actinomycetemcomitans. | serotype-specific polysaccharide antigens (spas) were extracted from whole cells of actinobacillus actinomycetemcomitans atcc 29523 (serotype a), y4 (serotype b), and nctc 9710 (serotype c) by autoclaving and purified by chromatography on deae-sephadex a-25 and sephacryl s-300 columns. y4 spa induced interleukin-1 (il-1) release by p388d1 murine macrophages. polymyxin b had virtually no effect on the release of il-1. rabbit anti-murine il-1 serum strongly suppressed the proliferation of c3h/hej ... | 1991 | 1987032 |
| immunogenicity of actinobacillus pleuropneumoniae outer membrane proteins and enhancement of phagocytosis by antibodies to the proteins. | to determine the opsonic effect of antibodies to actinobacillus (haemophilus) pleuropneumoniae outer membrane proteins on phagocytosis by porcine polymorphonuclear leukocytes (pmn), we separated the integral outer membrane proteins (iomps) by triton x-114 extraction. four major iomps with molecular masses of 76, 50, 39, and 29 kda were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. these iomps were found to be essentially free of endotoxin in the limulus amebocyte lysate ... | 1991 | 1987072 |
| subclass and molecular form of immunoglobulin a antibodies to actinobacillus actinomycetemcomitans in juvenile periodontitis. | patients with juvenile periodontitis frequently have elevated levels of serum immunoglobulin a (iga) antibodies to antigens of actinobacillus actinomycetemcomitans. iga occurs in two subclasses, iga1 and iga2, and in monomeric and polymeric forms. because iga1 is susceptible to cleavage by iga1 proteases produced by microorganisms found at mucosal sites and in the gingival crevice, we wished to determine the iga subclass distribution of antibodies to antigens of a. actinomycetemcomitans. the mol ... | 1991 | 1997415 |
| microbiological and immunological aspects of experimental periodontal disease in rats: a review article. | animal models in which microbiological and immunological aspects of periodontal disease can be studied prospectively seem well warranted. the rat bears much resemblance to man with respect to periodontal anatomy, development and composition of dental plaque, histopathology of periodontal lesions, and basic immunobiology. furthermore, reproducible methods are available for assessment of periodontal disease in rats, and detectable periodontal destruction can be induced in a few weeks in these anim ... | 1991 | 2002433 |
| microbiological study of hiv-related periodontitis. | the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ... | 1991 | 2002434 |
| isolation and partial characterization of a 39 kda major outer membrane protein of actinobacillus actinomycetemcomitans y4. | the outer membrane fractions of actinobacillus actinomycetemcomitans, which were extracted from whole cells with cetyl trimethyl ammonium bromide and cacl2, contained four major outer membrane proteins (momp) of 39, 37, 36 and 30 kda. the 39 kda momp of a. actinomycetemcomitans was sequentially purified by extraction with zwittergent 3-14 detergent, anion-exchange chromatography and gel filtration chromatography. analysis of amino acid composition and n-terminal amino acid sequence of 20 residue ... | 1991 | 2004699 |
| regulation of leukotoxin in leukotoxic and nonleukotoxic strains of actinobacillus actinomycetemcomitans. | actinobacillus actinomycetemcomitans is a gram-negative bacterium that has been implicated in the etiology of several forms of periodontitis, especially localized juvenile periodontitis. a potent leukotoxin (lkt) is produced by most a. actinomycetemcomitans isolates from patients with periodontal disease, but some isolates are leukotoxin nonproducing (lkt-). the molecular bases for the differences in leukotoxin expression are being explored to clarify the role of leukotoxin in pathogenesis. we h ... | 1991 | 2004819 |
| effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria. | chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ... | 1991 | 2005230 |
| serum antibody in actinobacillus actinomycetemcomitans-infected patients with periodontal disease. | this study was designed to (i) delineate the characteristics of serum antibody responses to actinobacillus actinomycetemcomitans in patients with periodontitis who are infected with a. actinomycetemcomitans; irrespective of disease classification; (ii) assess the relationship of the elevated antibody levels to colonization of the oral cavity by a. actinomycetemcomitans; and (iii) describe the serotype distribution of a. actinomycetemcomitans and antibodies to the microorganism in infected patien ... | 1991 | 2019443 |
| bacterial sampling by absorbent paper points. an in vitro study. | this in vitro study was designed to test the hypothesis that sampling of bacterial suspensions by absorbent paper points would accurately reflect the proportion of the bacterial species whether they are homogeneously or non-homogeneously distributed in the sample site. the non-homogeneously distributed populations consisted of equal numbers of colony forming units (cfu) of two periodontal bacterial species present as separate cultures, one layered on top of the other. the species in the top laye ... | 1991 | 2027062 |
| a randomized, placebo-controlled trial of doxycycline: effect on the microflora of recurrent periodontitis lesions in high risk patients. | twenty-seven patients with a recent history of periodontal abscesses and/or loss of gingival attachment level (gal) despite active periodontal therapy were enrolled in a double-blind, randomized, placebo-controlled trial. clinical measurements and subgingival scaling were performed every 2 months. when a site exhibited greater than or equal to 2 mm loss of gal or a periodontal abscess, patients were administered either doxycycline at a dosage of 200 mg to start and 100 mg per day for 3 weeks, or ... | 1991 | 2027071 |
| recovery of a. actinomycetemcomitans from teeth, tongue, and saliva. | the recovery of actinobacillus actinomycetemcomitans simultaneously from subgingival sites around teeth and dorsum of the tongue and/or saliva was examined in 293 subjects at 444 visits; 295 paired samples were available from subgingival sites and tongue, 171 paired samples from subgingival sites and stimulated saliva, and 137 paired samples from subgingival sites and unstimulated saliva. sixty-one subjects were periodontally healthy (mean age 20.3 years); 55 exhibited localized juvenile periodo ... | 1991 | 2027072 |
| intrafamilial transmission of actinobacillus actinomycetemcomitans. | cross-sectional studies have shown that actinobacillus actinomycetemcomitans is a frequent member of the oral flora in children with primary teeth. the purpose of the present study was to obtain information of the transmission of a. actinomycetemcomitans. three types of families were studied for the prevalence and serotype distribution of a. actinomycetemcomitans. first, families whose periodontally healthy child member harbored a. actinomycetemcomitans; second, families of periodontally healthy ... | 1991 | 2027073 |
| igg antibody response of localized juvenile periodontitis patients to the 29 kilodalton outer membrane protein of actinobacillus actinomycetemcomitans. | levels of serum igg antibody to the 29 kilodalton outer membrane protein of a. actinomycetemcomitans y4 in sera of periodontally healthy subjects and localized juvenile periodontitis patients were determined using an enzyme-linked immunosorbent assay (elisa). the 29 kda protein was isolated by solubilization of an octylglucoside-nacl insoluble fraction by incubation at ambient temperature in 20 mm sodium phosphate, ph 7.5, containing 1% sodium dodecyl sulfate. the isolated protein migrated on sd ... | 1991 | 2027074 |
| [subacute endocarditis on a prosthetic valve due to actinobacillus actinomycetemcomitans. a new classic]. | | 1991 | 2031104 |
| coagulation induced by challenge with various doses of a pleuropneumoniae in piglets. | | 1991 | 2031293 |
| oral associated bacterial infection in horses: studies on the normal anaerobic flora from the pharyngeal tonsillar surface and its association with lower respiratory tract and paraoral infections. | two hundred and seventy bacterial isolates were obtained from the pharyngeal tonsillar surface of 12 normal horses and 98 obligatory anaerobic bacteria were characterised. of these, 57 isolates belonging to 7 genera (peptostreptococcus (1); eubacterium (9); clostridium (6); veillonella (6); megasphera (1); bacteroides (28); fusobacterium (6)) were identified, and 16 of these were identified to species level (p. anaerobius (1); e. fossor (9); c. villosum (1); b. fragilis (1); b. tectum (2); b. he ... | 1991 | 2031304 |
| [determination of dna base composition and levels of dna-dna hybridization of actinobacillus actinomycetemcomitans]. | using the strains of actinobacillus actinomycetemcomitans (aa) and haemophilus aphrophilus (haph) in experiments, the mole percent quanine plus cytosine content (mol% g + c) and level of dna-dna hybridization (dna homology) were determined for the first time in oral microbiologic study in china. dna was extracted by marmur's method. the mol% g + c of dna was determined by thermal denaturation method (tm). dna-dna hybridization was carried out by de ley's optical procedure (initial renaturation r ... | 1991 | 2032476 |
| the cytotoxin of actinobacillus pleuropneumoniae (pleurotoxin) is distinct from the haemolysin and is associated with a 120 kda polypeptide. | mutants of actinobacillus pleuropneumoniae strain hk 361 (serotype 2) were isolated which were deficient in type ii (ca2(+)-dependent) haemolysin activity (hly-). some of the hly- mutants secreted a potent, heat-labile extracellular cytotoxic activity against porcine alveolar macrophages. comparison of cell-free culture supernatant from the parent strain and some hly- mutants by sds-page and immunoblotting revealed the loss of a major extracellular polypeptide of 109 kda. two hly- mutants which ... | 1991 | 2033378 |
| structure of the capsular polysaccharide from actinobacillus pleuropneumoniae serotype 7. | the structure of the capsular polysaccharide antigen of a. pleuropneumoniae serotype 7 was determined using 1d- and 2d-n.m.r. methods. dephosphorylation, methylation, and g.l.c.-mass spectrometry methods were used to confirm the analysis. the type-7 specific polysaccharide was a high-molecular-weight teichoic acid-type polymer of d-galactose, glycerol, and phosphate (2:1:1), composed of glycosylglycerol repeating units joined through monophosphate diester linkages and with the structure: [formul ... | 1991 | 2036652 |
| comparison and analysis of the nucleotide sequences of pilin genes from haemophilus influenzae type b strains eagan and m43. | previous studies have demonstrated antigenic differences among the pili expressed by various strains of haemophilus influenzae type b (hib). in order to understand the molecular basis for these differences, the structural gene for pilin was cloned from hib strain eagan (p+) and the nucleotide sequence was compared to those of strains m43 (p+) and 770235 b0f+, which had been previously determined. the pilin gene of hib strain eagan (p+) had a 648-bp open reading frame that encoded a 20-amino-acid ... | 1991 | 2037360 |
| specific antibody responses to subgingival plaque bacteria as aids to the diagnosis and prognosis of destructive periodontitis. | we have reviewed the recent literature on the humoral immune responses to a variety of subgingival plaque bacterial species in patients with destructive periodontal diseases. we do not feel that the information presently available on the specific antibody responses to proposed pathogens such as bacteroides gingivalis and actinobacillus actinomycetemcomitans allows antibody responses to be diagnostic. all control subjects without periodontal destruction have antibodies to candidate pathogens but ... | 1991 | 2045513 |
| a 2-year follow-up on the clinical and microbiological conditions of periodontium in teenagers. | 87 teenagers were included in a 2-year follow-up study on clinical and microbiological conditions of the periodontium. the clinical status was determined according to the community periodontal index of treatment needs (cpitn) for subjects under 20 years of age. 59% of the subjects had at least 3 healthy sextants at the baseline and 68% at the 2-year examination. the microbiological conditions were assessed by using direct dark-field microscopy, and the culture of actinobacillus actinomycetemcomi ... | 1991 | 2045514 |
| endothelial cytotoxicity of actinobacillus pleuropneumoniae. | the cytotoxicity of actinobacillus pleuropneumoniae serotype 1 strain cm5 for porcine and bovine endothelial cells in vitro, was dose-dependent. this strain and its attenuated and avirulent substrain cm5a were equally cytotoxic. the cytotoxicity observed during five hours of exposure of endothelial cells to bacterial products was abolished if the bacteria were inactivated by heat or sonication. exposure of the endothelial cells for five hours to 100 and 200 micrograms of purified lipopolysacchar ... | 1991 | 2047588 |