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biodegradation of pyrene and phenanthrene in soil using immobilized fungi fusarium sp. 200516385948
[biological preparations with different mechanism of action for protecting potato against fungal diseases].mycological analysis throughout the vegetation period of potato (solanum tuberosum) made it possible to study in detail the structure of micromycete community, to determine typical dominant (frequency, more than 60%), typical common (frequency, 30 to 60%), typical rare (frequency, 10 to 30%), and casual (frequency, less than 10%) species and to estimate changes in the microorganism community caused by plant protection preparations with different mechanisms of action. it was shown that, as a resu ...200616521583
mycotoxin production by fusarium species isolated from bananas.the ability of fusarium species isolated from bananas to produce mycotoxins was studied with 66 isolates of the following species: f. semitectum var. majus (8 isolates), f. camptoceras (3 isolates), a fusarium sp. (3 isolates), f. moniliforme (16 isolates), f. proliferatum (9 isolates), f. subglutinans (3 isolates), f. solani (3 isolates), f. oxysporum (5 isolates), f. graminearum (7 isolates), f. dimerum (3 isolates), f. acuminatum (3 isolates), and f. equiseti (3 isolates). all isolates were c ...199716535503
antimicrobial activities of cinnamon oil and cinnamaldehyde from the chinese medicinal herb cinnamomum cassia blume.both cinnamomum verum j.s. presl. and cinnamomum cassia blume are collectively called cortex cinnamonmi for their medicinal cinnamon bark. cinnamomum verum is more popular elsewhere in the world, whereas c. cassia is a well known traditional chinese medicine. an analysis of hydro-distilled chinese cinnamon oil and pure cinnamaldehyde by gas chromatography/mass spectrometry revealed that cinnamaldehyde is the major component comprising 85% in the essential oil and the purity of cinnamaldehyde in ...200616710900
breakthrough fusarium sp probable pneumonia during fluconazole therapy in an aids patient with diabetes, candidemia, pneumocystis carinii pneumonia and cytomegalovirus disseminated infection. 200616736895
post-traumatic fungal endophthalmitis--a prospective study.to study the incidence, clinical presentation, and the response of anti-fungals in cases of fungal endophthalmitis following open globe injury.200816751752
enantioselective baeyer-villiger oxidation of bicyclo[3.2.0]hept-2-en-6-one with fungi: optimization of biotransformation and use of tio2 as support of cell growth.fungi from amazonian forest soil (ecuador) and an italian factory were screened for baeyer-villiger (bv) oxidation of bicyclo [3.2.0]hept-2-en-6-one to 2-oxabicyclo[3.3.0]oct-6-en-3-one (corey's lactone). isolates of fusarium sp. and f. solani produced the (+)-(1r,5s)-lactone while isolates of aspergillus terricola and a. amazonicus afforded the (-)-(1s,5r)-lactone. highest conversions (85% yield and 70% enantiomeric excess) were obtained with a. amazonicus grown in presence of 2.7 mm titanium d ...200616786245
[skin infection with fusarium in an immunocompetent patient].fusarium spp. are fungi found throughout the world and can cause a great variety of skin infections, mainly in immunodepressed individuals. we present a case of skin infection with fusarium sp. which manifested as painful superficial ulcers on the legs of an immunocompetent female patient, who had applied <<medicinal mud>> as a <<natural remedy>> for leg pain. the condition was cured with oral itraconazole and local treatments.200616801025
diagnosis of fusariosis in urine cytology.fusarium is a filamentous fungus widely distributed in plants and in the soil. most species are more common at tropical and subtropical areas. besides being a common contaminant and a well-known plant pathogen, fusarium sp may cause various infections in humans. however, it has not yet been reported as being the pathogen of urinary tract infection. a 67-year-old woman had extracorporeal shock wave lithotripsy and percutaneous nephrolithotomy for renal stones 7 and 6 years ago, respectively. she ...200716816172
review of epidemiology, diagnosis, and treatment of invasive mould infections in allogeneic hematopoietic stem cell transplant recipients.invasive mould infections are a major cause of morbidity and mortality in hematopoietic stem cell transplant recipients (hsct). allogeneic hsct recipients are at substantially higher risk than autologous hsct recipients. although neutropenia following the conditioning regimen remains an important risk factor for opportunistic fungal infections, most cases of invasive mould infection in allogeneic hsct recipients occur after neutrophil recovery in the setting of potent immunosuppressive therapy f ...200616830186
antimicrobial activity of vanillin against spoilage microorganisms in stored fresh-cut mangoes.the antimicrobial activity of vanillin against four bacteria (pantoea agglomerans, aeromonas enteropelogenes, micrococcus lylae, and sphingobacterium spiritovorun), four fungi (alternaria sp., aspergillus sp., penicillium sp., and fusarium sp.), and three unidentified yeasts isolated from spoiling fresh-cut mango slices was verified in laboratory media adjusted to ph 5.0. mics of vanillin against the fungi (12.5 to 13.3 mm), bacteria (10 to 13.3 mm), and yeasts (5.0 to 6.7 mm) indicated that all ...200616865911
a comparative study on biosorption characteristics of certain fungi for bromophenol blue dye.laboratory investigations of the potential use of dried biomasses of rhizopus stolonifer, fusarium sp., geotrichum sp., and aspergillus fumigatus as biosorbents for the removal of bromophenol blue (bpb) dye from aqueous solutions were conducted. kinetics studies indicated that the bpb dye uptake processes can be well described by the pseudo-second-order model. the fungal biomasses exhibited the highest dye biosorption at ph 2.0. the langmuir adsorption model appears to fit the dye biosorption be ...200616891666
biodegradation of benzo(a)pyrene by a newly isolated fusarium sp.benzo(a)pyrene (bap) is a five-ring polycyclic aromatic hydrocarbon produced by the incomplete combustion of organic materials. it is one of the priority pollutants listed by the us environmental protection agency. this study describes a fungal isolate that is able to biodegrade benzo(a)pyrene. the filamentous fungus, isolated from leaves of pterocarpus macrocarpus kurz., was identified as a fusarium sp. (strain e033). fusarium sp. e033 was able to survive in the presence of benzo(a)pyrene conce ...200616907745
purification and characterization of an exochitinase from bacillus thuringiensis subsp. aizawai and its action against phytopathogenic fungi.a chitinolytic enzyme from bacillus thuringiensis subsp. aizawai has been purified and its molecular mass was estimated ca. 66 kda by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). the enzyme was able to hydrolyze chitin to chitobiosides but not carboxymethylcellulose, cellulose, pullulan, and laminarin. optimal ph and temperature were detected at 6 and 50 degrees c, respectively. stability, in the absence of substrate, was observed at temperatures less than 60 degrees c a ...200616917521
[in vivo confocal microscopy in the diagnosis of fungal keratitis: case report].the authors describe a case of fungal keratitis that the in vivo confocal microscopy helped in the diagnosis and follow-up. confocal microscopy was done in a patient's ulcer that did not improve with several topical medicines. corneal scrapings were obtained and culture results were without conclusion. we observed hyphae and infectious collections on confocal microscopy. new corneal culture showed fusarium sp ten days after confocal diagnosis.200616936966
sensitive detection of estrogenic mycotoxin zearalenone by open sandwich immunoassay.zearalenone (zea) is an estrogenic mycotoxin produced by fusarium sp., and its production on corn and small grains during storage has been of considerable concern. for sensitive zea detection, we applied an open sandwich (os) immunoassay that can noncompetitively detect monovalent antigens utilizing an antigen-induced enhancement of the v(h)/v(l) interaction. we cloned the v(h) and v(l) cdnas of anti-zea mab to a split-fv phagemid pkst2, and firstly both v(h) and v(l) fragments were displayed on ...200717213626
purification and characterization of fibrinolytic alkaline protease from fusarium sp. blb.fusarium sp. blb, which produces a strongly fibrinolytic enzyme, was isolated from plant leaf (hibiscus). fibrinolytic alkaline protease was purified from a culture filtrate of fusarium sp. blb by precipitation with (nh4)2(so4) and column chromatography with cm-toyopearl 650 m and superdex 75. the purified enzyme was homogeneous on sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the molecular weight was 27,000 by sds-page. maximum activity of protease was observed at ph 9.5 ...200717221202
purification and characterization of a beta-1,3-glucanase from the novel mycoparasite periconia byssoides.an extracellular beta-1,3-glucanase with antifungal properties was secreted by the novel mycoparasite, periconia byssoides. the glucanase has a molecular mass of 35 kda estimated by sds-page. its optimum activity was at ph 6.0 and 50 degrees c (over 2 h). the purified beta-1,3-glucanase was capable of degrading cell walls, and inhibiting mycelia growth and spore germination of plant pathogenic fungi including fulvia fulva, fusarium sp. and rhizoctonia solani. the n-terminal amino acid residues o ...200717242851
isolation and structure elucidation of neofusapyrone from a marine-derived fusarium species, and structural revision of fusapyrone and deoxyfusapyrone.three polyketides containing a pyrone ring, neofusapyrone, fusapyrone, and deoxyfusapyrone, were isolated from the marine-derived fungus fusarium sp. fh-146. their structures were determined by extensive id- and 2d-nmr and ms spectral analyses. fusapyrone and deoxyfusapyrone were originally reported as a-pyrone derivatives; however, the revised structures indicate that they are gamma-pyrone derivatives. these three compounds exhibited antimicrobial activities.200617256469
the characteristics and mechanisms of phenol biodegradation by fusarium sp.fusarium sp. hj01 can grow using phenol as only carbon resource and has strong ability of phenol degradation. the effect of ph, temperature and sucrose addition on biodegradative capacity of fusarium sp. hj01 was examined. the main metabolism pathways and mechanism of phenol degradation by hj01 strain is described. this strain exhibited both cathecol 1,2-dioxygenase (c12) and cathecol 2,3-dioxygenase (c23) in free cell extracts obtained from cells grown exclusively on phenol or with sucrose adde ...200717336453
fungal keratitis in melbourne.description of the clinical and microbiological spectrum of fungal keratitis at a tertiary eye care hospital in melbourne, australia.200717362452
in-silico docking of hiv-1 integrase inhibitors reveals a novel drug type acting on an enzyme/dna reaction intermediate.hiv-1 integrase (in) is an emerging drug target, as in strand transfer inhibitors (instis) are proving potent antiretroviral agents in clinical trials. one credible theory sees instis as docking at the cellular (acceptor) dna-binding site after in forms a transitional complex with viral (donor) dna. however, mapping of the dna and insti binding sites within the in catalytic core domain (ccd) has been uncertain.200717374162
a case of mycotic endophthalmitis after triamcinolone acetonide-assisted pars plana vitrectomy.only two cases of bacterial endophthalmitis after triamcinolone acetonide (ta)-assisted pars plana vitrectomy (ppv) have been reported. as far as we are aware there has been no report of fungal endophthalmitis occurring after ta-assisted ppv. we report a case of endophthalmitis due to fusarium after ta-assisted ppv. an otherwise healthy 61-year-old woman suffered from branch retinal vein occlusion with macular edema, which led to reduced visual acuity and metamorphopsia. because she complained o ...200717393075
multicenter evaluation of a new disk agar diffusion method for susceptibility testing of filamentous fungi with voriconazole, posaconazole, itraconazole, amphotericin b, and caspofungin.the purpose of this study was to correlate inhibition zone diameters, in millimeters (agar diffusion disk method), with the broth dilution mics or minimum effective concentrations (mecs) (clsi m38-a method) of five antifungal agents to identify optimal testing guidelines for disk mold testing. the following disk diffusion testing parameters were evaluated for 555 isolates of the molds absidia corymbifera, aspergillus sp. (five species), alternaria sp., bipolaris spicifera, fusarium sp. (three sp ...200717428932
in vitro antifungal activity of extract and plumbagin from the stem bark of diospyros crassiflora hiern (ebenaceae).in this study the methanol/dichloromethane (1:1) extract and plumbagin isolated from extract of stem barks of diospyros crassiflora were tested for their antifungal activity against 12 strains of yeast pathogens and filamentous fungi: candida albicans, candida glabrata, candida krusei, candida tropicalis, cryptococcus neoformans, aspergillus niger, aspergillus flavus, alternaria sp., cladosporium sp., geotrichum candidum, fusarium sp. and penicillium sp. the growth of all fungi strains tested wa ...200717444575
fusaristatins a and b, two new cyclic lipopeptides from an endophytic fusarium sp.two new cyclic lipopeptides, fusaristatins a (1) and b (2) were isolated from rice cultures of a fusarium sp. yg-45 in the course of a screening of endophytic fungi. their structures of 1 and 2 were determined by spectroscopic methods. 2 showed a moderate inhibitory effect on topoisomerases i (ic50: 73 microm) and ii (ic50: 98 microm) without cleavable complexes. furthermore, 1 and 2 showed the growth-inhibitory activity toward lung cancer cells lu 65 with ic50 values of 23 and 7 microm, respect ...200717551209
mycoflora of two types of portuguese dry-smoked sausages and inhibitory effect of sodium benzoate, potassium sorbate, and methyl p-hydroxybenzoate on mold growth rate.the mycoflora of chouriqo types alentejano and ribatejano, two varieties of portuguese dry-smoked sausages, have been investigated after a producer-defined shelf life period (120 days at 20 +/- 5 degrees c) in modified atmosphere packaging (55% n2 and 45% co2). on the basis of morphological and physiological characteristics, the isolates were identified as penicillium, aspergillus, fusarium, rhizopus, monilia, absidia, and cephalosporium. the species identified were as follows: penicillium terre ...200717612078
effects of irradiation on fungi and fumonisin b(1) in corn, and of microwave-popping on fumonisins in popcorn.fumonisins are metabolites produced in corn primarily by the fungus fusarium verticillioides (f. moniliforme) and are toxic to humans and animals. fumonisin b(1) (fb(1)) is the primary fumonisin produced and is found frequently in corn kernels, some of which may be used as food or food ingredients. a three-part study was conducted to determine the effects of gamma- and electron beam irradiation on the levels of fumonisins in naturally contaminated field corn, and the effects of microwave-popping ...200717613059
[isolation of filamentous fungi from water used in a hemodialysis unit].despite the relative frequency of opportunistic fungal infections among hemodialysis patients, the reservoirs for these microorganisms in the environment remain unknown, although some recent studies have made correlations with the water supply as their source. the objective of the present study was to monitor the mycological quality of the water system of a hemodialysis unit in the interior of the state of são paulo, brazil, over the period from april to july 2006. fifteen samples of 1000 ml wer ...200717653470
heterologous expression of new antifungal chitinase from wheat.chitinases (ec 3.2.1.14) have been grouped into seven classes (class i-vii) on the basis of their structural properties. chitinases expressed during plant-microbe interaction are involved in defense responses of host plant against pathogens. in the present investigation, chitinase gene from wheat has been subcloned and overexpressed in escherichia coli bl-21 (de3). molecular phylogeny analyses of wheat chitinase indicated that it belongs to an acidic form of class vii chitinase (glycosyl hydrola ...200717697785
ycm1008a, a novel ca2+-signaling inhibitor, produced by fusarium sp. ycm1008.in the course of screening for drugs that suppress the ca(2+)-mediated growth inhibition in a yeast mutant, we found that the metabolite of fusarium sp. strain ycm1008 inhibited ca(2+)-signaling. a novel pyrano-pyridone, ycm1008a was isolated from the fermentation broth using hlb column chromatography followed by hplc, and the structure was elucidated by spectral analysis. ycm1008a suppressed ca(2+)-induced growth inhibition of the saccharomyces cerevisiae (deltazds1deltasyr1) mutant.200717721004
[fungic microbiota of normal conjunctiva, sugar-cane and anemophilous fungi of the region of monte belo - minas gerais].to evaluate the incidence of fungi in the ocular conjunctiva of sugar-cane cutting workers as well in a sugar-cane plantation environment.200717768551
[studies on purification and properties of antagonistic protein from bacteria ss02 of paenibacillus daejeonensis].the antifungal, anti-bacterical, anti-brine shrimp activities of sd22 isolated from paenibacillus daejeonensis bacteria ss02 were studied. the separation steps included ultracentrifugation, ultrafiltration and (nh4)2so4 fractional precipitation, further purification was performed by sephadexg-75 and deae-32 chromatography. its molecular weight determined by sds-page was 56.0 kd and its isoelectfic point was 6.4. sd22 was thermostable to some extent and stable to ultraviolet, but sensitive to som ...200717822044
the fibrinolytic activity of a novel protease derived from a tempeh producing fungus, fusarium sp. blb.tempeh is a traditional indonesian soybean-fermented food produced by filamentous fungi, rhizopus sp. and fusarium sp. we isolated and sequenced the genomic gene and a cdna clone encoding a novel protease (fp) from fusarium sp. blb. the genomic gene was 856 bp in length and contained two introns. an isolated cdna clone encoded a protein of 250 amino acids. the predicted amino acid sequence of fp showed highest homology, of 76%, with that of trypsin from fusarium oxysporum. the hydrolysis activit ...200717827689
coprinellus curtus (hitoyo-take) prevents diseases of vegetables caused by pathogenic fungi.a strain of coprinellus curtus (designated gm-21), a basidiomycete that suppressed bottom-rot disease of chinese cabbage, 'pak-choi' (brassica campestris), caused by the pathogen rhizoctonia solani pak-choi 2 was isolated. the mechanism of plant disease suppression was discovered to be hyphal interference, a combative fungal interaction between strain gm-21 and the pathogen. the antifungal spectrum of strain gm-21 was shown to include r. solani and fusarium sp., i.e. strain gm-21 showed disease- ...200717850327
phytophthora cinnamomi and other fine root pathogens in north temperate pine forests.a number of fine root pathogens, including phytophthora cinnamomi, pythium ultimum var. ultimum, pythium undulatum, pythium violae, fusarium sp., and two incompletely identified verticillium species, were isolated from soils taken from under scots pine trees at five sites in north scotland, including semi-natural forests and plantations. at least two root pathogens were recovered from each forest. morphological and molecular data supported the identification of phytophthora cinnamomi from three ...200717937665
antifungal activity of a novel compound from burkholderia cepacia against plant pathogenic fungi.to investigate antifungal activity of a novel compound (named as cf66i provisionally) against plant pathogenic fungi, mainly including fusarium sp., colletotrichum lindemuthianum, rhizoctonia solani, etc.200717958556
diversity in antifungal activity of strains of chromobacterium violaceum from the brazilian amazon.chromobacterium violaceum is a free-living gram-negative bacterium found in soil and aquatic habitats; abundantly present in the brazilian amazon, it is an important example of exploitable microbial diversity of the tropics. in this study, 24 strains from the brazilian amazon and atcc 12472(t) were investigated for biocontrol potential of seven fungi pathogenic to soybean [glycine max (l.) merril] seed. both cells and the supernatants of two brazilian strains, 07-1 and 27-1, together with atcc 1 ...200818347828
disseminated fusarium infection in a multiple trauma patient.the hyalohyphomycetes (especially fusarium sp.) have emerged as significant pathogens in severely immunocompromised patients. human infections by fusarium sp. can be superficial or limited to single organs in otherwise healthy patients. such infections are rare and tend to respond well to therapy. by contrast, disseminated fusarial hylohyphomycosis affects the immunocompromised host and frequently is fatal. successful outcome is determined by the degree of immunosuppression and the extent of the ...200818364666
[diversity of facultatively anaerobic microscopic mycelial fungi in soils].the numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of cfu per one gram of soil; a total of 30 species was found. this group is composed primarily of mitotic fungi of the ascomycete affinity belonging to the orders hypocreales (fusarium solani, f. oxysporum, fusarium sp., clonostachys grammicospora, c. rosea. acremonium sp., gliocladium penicilloides, trichoderma aureoviride, t. harzianum, t. polysporum, t. viride. t. koning ...200818365728
detection of phld gene in some fluorescent pseudomonads isolated from iran and its relative with antifungal activities.fluorescent pseudomonads that produce antibiotic 2,4-diacetylphloroglocinol (2,4-dapg) are important group of pgrp that inhibit a broad spectrum of plant pathogenic fungi. studying on genetic diversity of 2,4-diacetylphloroglucinol-producing fluorescent pseudomonads has been shown with special importance. the first step to investigate the genetic diversity of these bacteria is detecting of the genes required for the biosynthesis of this antibiotic. the objectives of the current study were detect ...200718396832
biotransformation of (+/-)-2-methylcyclohexanone by fungi.the biotransformation of 2-methylcyclohexanone (1) using 16 fungal strains and some mushroom cultures was investigated. fusarium sp. was one of the effective biocatalysts for oxidoreduction of 2-methylcyclohexanone (1). cis-2-methylcyclohexanol (2a) was isomerized to trans-2-methylcyclohexanol (2b) by fusarium sp. in addition, the corresponding lactones 3 was obtained by baeyer-villiger oxidation using fusarium sp. ap-2 (46%, 94% ee).200818427929
effect of the time interval from harvesting to the pre-drying step on natural fumonisin contamination in freshly harvested corn from the state of parana, brazil.natural mycoflora and fumonisins were analysed in 490 samples of freshly harvested corn (zea mays l.) (2003 and 2004 crops) collected at three points in the producing chain from the northern region of parana state, brazil, and correlated to the time interval between the harvesting and the pre-drying step. the two crops showed a similar profile concerning the fungal frequency, and fusarium sp. was the prevalent genera (100%) for the sampling sites from both crops. fumonisins were detected in all ...200818473218
[filamentous fungi preservation in distilled water.].some methods for keeping the fungal culture collection have been used. however, the choice of either one on the basis that must ensure the cultural genetic stability and its phenotipic characteristics. in this work the preservation method in distilled water recognized in the literature as a single, economic and certain method that guarantie the survival of fungus cultures for long periods was used. 26 strains of generus and species: aspergillus niger, aspergillus candidus, fusarium sp., fusarium ...199818473541
the use of its dna sequence analysis and maldi-tof mass spectrometry in diagnosing an infection with fusarium proliferatum.although mycoses are among the most common diseases worldwide, infections with fusarium spp. occur only rarely. mostly patients suffering from underlying immune deficiency are infected with this mould, resulting in a considerably decreasing prognosis. in immunocompromised patients, cutaneous manifestations are more often associated with fusarium sp. than with candida sp. or aspergillus sp. we describe one patient with acute lymphoblastic leukaemia, who was first treated with chemotherapy after g ...200818547323
development of a novel pcr assay for the identification of the black yeast, exophiala (wangiella) dermatitidis from adult patients with cystic fibrosis (cf).cystic fibrosis (cf) patients may suffer increased morbidity and mortality through colonisation, allergy and invasive infection from fungi. the black yeast, exophiala dermatitidis (synonym wangiella dermatitidis) has been found with increasing frequency in sputum specimens of cf patients, with reported isolation rates ranging from 1.1 to 15.7%. at present, no diagnostic pcr exists to aid with the clinical laboratory detection and identification of this organism. a novel species-specific pcr-base ...200818571996
mold challenge study in bottled natural mineral waters and spring waters.microbiological challenge study was carried out to verify the microbial stability of bottled waters against four different mold species isolated from bottled water (fusarium sp.; cladosporium sp.; penicillium chrysogenum and aspergillus fumigatus) and to follow the growth of the molds in bottled water. twelve types of bottled water with different mineralization and co2 level in pet and glass packages were collected from 4 european countries. three different inoculation levels of spore suspension ...200818595319
acanthamoebicidal activity of fusarium sp. tlau3, an endophytic fungus from thunbergia laurifolia lindl.a fungal endophyte identified as fusarium sp. tlau3 was isolated from fresh twig of thunbergia laurifolia lindl., a thai medicinal plant collected from the forest of chiang mai province, northern thailand. the fungus was grown on a medium containing yeast extracts and sucrose. the fungal metabolites were isolated from the culture broth by dichloromethane extraction, isooctane/methanol then n-butanol/water partitions, and fractionation with sephadex lh 20 column chromatography. acanthamoebicidal ...200818633646
importance of seed-borne fungi of sorghum and pearl millet in burkina faso and their control using plant extracts.seed-borne fungi of sorghum and pearl millet in burkina faso were surveyed. a total of 188 seed samples from various locations, collected in 1989 (42) and 2002 (146), were tested, using the blotter, dry inspection and washing methods. infection experiments were carried out with the major fungi recorded on each crop by the blotter test. six essential oils of plants were investigated for their inhibitory activity against eight pathogenic fungi. thirty four and 27 fungal species were found in seed ...200818817152
diversity of root associated microorganisms of selected medicinal plants and influence of rhizomicroorganisms on the antimicrobial property of coriandrum sativum.the total heterotrophic bacteria, actinomycetes and fungus were enumerated from the rhizosphere and non-rhizosphere soil of 50 selected locally available medicinal plants in and around bharathiar university. in all the plants, population of microorganism were higher in the rhizosphere soil than in the non rhizosphere soil. among the microorganisms, bacterial population was higher in number followed by fungus and actinomycetes. of the medicinal plants, the maximum rhizosphere effect was observed ...200818831345
mango malformation disease and the associated fusarium species.abstract mango malformation disease (mmd) occurs in asia, africa, and the americas and was first reported in india in 1891. the vegetative form of mmd was first reproduced in 1966 with fusarium moniliforme and the floral form with isolates of f. moniliforme var. subglutinans from both vegetative shoots and floral tissue. the fungi were subsequently recognized as f. subglutinans. in 2002, a new species, f. mangiferae, was established based on nuclear and mitochondrial dna sequences; it included s ...200618943188
production and characterization of a monoclonal antibody raised against surface antigens from mycelium of gaeumannomyces graminis var. tritici: evidence for an extracellular polyphenol oxidase.abstract a murine monoclonal antibody (mab) of immunoglobulin class m (igm) was raised against surface antigens from gaeumannomyces graminis var. tritici and, by enzyme-linked immunosorbent assay, recognized isolates of g. graminis var. tritici, g. graminis var. avenae and g. graminis var. graminis. characterization of the antigen by heat and protease treatments showed that the epitope recognized by the mab was a protein. antigen production was detected only in live mycelia. immunofluorescence s ...199718945163
fusarium keratitis associated with renu with moistureloc sample kits.to describe a patient who developed contact-lens associated fusarium sp. keratitis associated with use of sample kits of renu with moistureloc purchased after worldwide recall of the product from the market.200818997545
soil solarization: a safe, affective and practicable technique for the control of soil born fungi and nematodes.a technique i.e., soil solarization and amendments (neem, chicken farmyard manure, farmyard manure and biokhad viz synthetic bio fertilizer), towards the natural cropping system has been evaluated for its effectiveness and practicability at the national agricultural research center islamabad pakistan. soil solarization and amendments were analyzed as a control measure against soil born fungi and nematodes. eight weeks of solarization resulted in about 11 degrees c increase in the soil temperatur ...200719069986
mycoflora of maize harvested from iran and imported maize.the natural occurrence of fungal contamination was evaluated in stored maize in three different agro-ecological zones (iran, brazil and china). a total of 45 samples were analyzed and 685 fungal isolates were identified. the most frequent isolated fungi from maize originated from iran, brazil and china were fusarium sp. (17.3, 17.9 and 37.1%), aspergillus sp. (9.3, 17.4 and 19.7%),penicillium sp. (5.8, 15.2 and 17.6%), rhizopus sp. (2.4, 3.2 and 3.5%), mucor sp. (1.1, 1.6 and 1.3%), cladosporium ...200719093507
utility of molecular sequence analysis of the its rrna region for identification of fusarium spp. from ocular sources.fungal ocular infections cause significant ocular morbidity, particularly when diagnosis and treatment are delayed. accurate morphologic identification of fusarium spp. beyond the genus is time-consuming and insensitive. it was the purpose of this study to examine the usefulness of the nuclear ribosomal rna (rrna) internal transcribed spacer regions (its1 and -2) to detect and differentiate fusarium spp. responsible for ocular infections.200919136697
purification and characterization of a novel fibrinolytic protease from fusarium sp. cpcc 480097.a novel fibrinolytic enzyme from fusarium sp. cpcc 480097, named fu-p, was purified to electrophoretic homogeneity using ammonium sulfate precipitation and ion exchange and gel filtration chromatography. fu-p, a single protein had a molecular weight of 28 kda, which was determined by sds-page and gel filtration chromatography. the isoelectric point of fu-p determined by isoelectric focusing electrophoresis (ief) was 8.1, and the optimum temperature and ph value were 45 degrees c and 8.5, respect ...200919142678
onychomycosis caused by fusarium sp in sri lanka: prevalence, clinical features and response to itraconazole pulse therapy in six cases.the aims of our study were to ascertain the proportion of fusarium onychomycosis among patients with suspected onychomycosis, to record clinical features and to assess the efficacy of itraconazole pulse therapy in treatment.200819160539
structure elucidation and nmr assignments for three anthraquinone derivatives from the marine fungus fusarium sp. (no. zh-210).the structure elucidations and complete (1)h and (13)c nmr assignments are reported for three new anthraquinone derivatives: fusaquinon a (1), b (2), and c (3) isolated from the fermentation medium of the marine fungus fusarium sp. (no. zh-210). hreims, fourier transform infrared absorption spectrometry (ft-ir), nmr experiments including gcosy, ghmqc, ghmbc, and noesy were used for the determination of the structures and nmr spectral assignments. preliminary pharmacological test showed that they ...200919165844
screening of endophytic fungi with antithrombotic activity and identification of a bioactive metabolite from the endophytic fungal strain cpcc 480097.fermentation extracts from 1,075 endophytic fungi were screened by the markwardt method. the endophytic strain cpcc 480097 had the strongest antithrombotic activity and was identified as fusarium sp. based on morphologic tests and internal transcriptional spacer sequence analysis. the target of the antithrombotic agent from the endophytic strain cpcc 480097 was identified by analysis of the fibrinogen clotting time, amidolytic activity, and fibrinolytic assay. the results showed that this antith ...200919198939
scanning electron microscopy applied to seed-borne fungi examination.the aim of this study was to test the standard scanning electron microscopy (sem) as a potential alternative to study seed-borne fungi in seeds, by two different conditions of blotter test and water restriction treatment. in the blotter test, seeds were subjected to conditions that enabled pathogen growth and expression, whereas the water restriction method consisted in preventing seed germination during the incubation period, resulting in the artificial inoculation of fungi. in the first condit ...200919204924
epidemiological and mycological data of onychomycosis in goiania, brazil.onychomycosis defined as fungal infection of the nail represents more than 50% of all onychopathies. epidemiological studies have shown that this mycosis is worldwide in occurrence, but with geographical variation in distribution. the direct microscopy and culture of the nail samples were performed to identify the causative agent. out of 2273 patients with nail infection examined between january 2000 and december 2004 in goiania, state of goias, brazil, diagnosis of onychomycosis was confirmed i ...201019207842
diseases on wheat and triticale under the growing conditions of lubumbashi (congo rd).the food production in katanga is inadequate and causes food insecurity for more than 400.000 people. nowadays the katanga province relies for more than 75 % on imported wheat and wheat flour from neighbouring countries. the absence of adapted germplasm is one of the mean reasons for the low production levels of small grains and as consequence the disinteresting of farmers for producing wheat. to select well adapted genotypes a screening test with wheat (t. aestivum) and triticale (x triticoseca ...200819226746
synthesis, characterization and antifungal activities of 3d-transition metal complexes of 1-acetylpiperazinyldithiocarbamate, m(acpdtc)2.a series of mononuclear 3d-transition metal complexes of the type m(acpdtc)2 have been synthesized (where acpdtc=1-acetylpiperazinyldithiocarbamate, m=mn(ii), fe(ii), co(ii), ni(ii) and cu(ii)). the ligand and its complexes have been characterized by micro analysis (chns), tg/dsc, ft-ir, uv-vis, 1h nmr, magnetic susceptibility and conductance measurements. on the basis ir spectroscopy a symmetrical bidentate coordination has been observed for the 1-acetylpiperazinyldithiocarbamate moiety in all ...200919230748
structural studies on minor enniatins from fusarium sp. vi 03441: novel n-methyl-threonine containing enniatins.a strain of a yet unidentified fusarium sp. produced in addition to enniatins a1, b and b1 a number of minor enniatins. the strain formed a well supported clade with strains identified as fusarium acuminatum (gibberella acuminata) in phylogenetic analyses using the tef-1alpha gene sequences. two of the minor enniatins were easily recognised as hydroxylated species on the basis of their fragment ion spectra. the hydroxylation could be traced to one of the amino acid moieties using multiple-stage ...200919249325
improving enzymatic production of ginsenoside rh2 from rg3 by using nonionic surfactant.in this study, several nonionic surfactants were tried to improve the enzymatic hydrolysis of ginsenoside rg3 into rh2 which was catalyzed at 50 degrees c and ph 5.0 by a crude glucosidase extracted from fusarium sp. ecu2042. among the biocompatible nonionic surfactants, polyethylene glycol 350 monomethyl ether was shown to be the best. after optimizing some influencing factors on the reaction, the conversion of rg3 (5 g/l) with 10 g/l crude enzyme reached almost 100% in the presence of the noni ...201019263249
benomyl tolerance of ten fungi antagonistic to plant-parasitic nematodes.ten strains of fungi were tested for tolerance to the fungicide benomyl. verticillium chlamydosporium strain 2 did not grow in the presence of benomyl; drechraeria coniospora strains 1 and 2 and chaetomium sp. tolerated only 0.1 mug benomyl/ml medium; acremonium bacillisporum, an unidentified fungus, and phoma chrysanthemicola uniformly grew at 1 mug/ml, but some hyphae grew at higher benomyl concentrations; fusarium sp. tolerated 475 mug/ml, but some hyphae grew on medium amended with 1,000 mug ...199119283145
isolation of fungi from heterodera glycines and in vitro bioassays for their antagonism to eggs.twenty fungi were assayed in vitro for antagonism to eggs of heterodera glycines. eight of the fungi were isolated from cysts or eggs of h. glycines during the current study, one was isolated from panagrellus redivivus, and eleven were obtained from other researchers or collections. the bioassays were conducted on eggs from nematodes that had been grown monoxenically on excised root tips. phoma chrysanthemicola, one strain of verticillium chlamydosporium, and one strain of v. lecanii caused a de ...199019287754
transforming a nep1 toxin gene into two fusarium spp. to enhance mycoherbicide activity on orobanche--failure and success.the nep1 gene encoding a fungal toxin that successfully conferred hypervirulence when transformed into colletotrichum coccodes (wallr.) hughes attacking abutilon theophrasti (l.) medic. was tested to ascertain if it would enhance pathogenicity of fusarium species to orobanche aegyptiaca pers. parasitising crops.200919291699
epidemiologic aspects and clinical outcome of fungal keratitis in southeastern brazil.fungal keratitis (fk) is a sight-threatening disease, more prevalent in developing regions. the present retrospective study was conducted in order to evaluate the epidemiologic and clinical aspects and the progression of fk in patients treated at two ophthalmologic reference centers in southeast brazil.200919396778
fungal endophytes from acer ginnala maxim: isolation, identification and their yield of gallic acid.the aim of the study was to isolate the endophytic fungi from acer ginnala and screen isolates rich in gallic acid.200919413766
[risk factor for lifestyle and way of living for symptoms of sick building syndrome: epidemiological survey in japan].to investigate the association among symptoms of sick building syndrome (sbs). self-reported questionnaire and indoor environmental surveys of newly build dwellings in japan were conducted.200919502765
assessment of bacterial and fungal growth on natural substrates: consequences for preserving caves with prehistoric paintings.the most representative bacterium (pseudonocardia sp.) and fungus (fusarium sp.) from the microbial communities of a cave containing paleolithic paintings were isolated and their growth on natural substrates assessed. growth was tested at the in situ and optimal, laboratory growth temperature. development was analyzed with and without supplemented nutrients (glucose, ammonium, phosphate, peptone). results showed that the assayed bacterium on natural substrate was able to develop best at in situ ...200919536596
infections in patients with multiple myeloma.infectious diseases are the leading causes of death in patients with multiple myeloma (mm). major changes have occurred in the spectrum of infections paralleling the changes in the treatment of mm. bacteria (particularly gram-negative organisms) are the most frequent etiologic agents, and invasive fungal infections caused by molds (aspergillus sp. and fusarium sp.) have been increasingly reported. new infections have emerged with an increase in the intensity of the treatment of mm. management of ...200919549580
combined effects of cu, cd, pb, and zn on the growth and uptake of consortium of cu-resistant penicillium sp. a1 and cd-resistant fusarium sp. a19.microorganisms can be important biosorbents for heavy metal remediation of contaminated soils and wastewaters. with different types and concentrations of heavy metals, strains display different resistance and removal abilities to the heavy metals. the objective of this study was to investigate the effects of single and multiple heavy metals on the growth and uptake of consortium of two types of fungal strains, penicillium sp. a1 and fusarium sp. a19. these fungal strains were tested to be tolera ...200919592158
mycotoxicological quality evaluation of corn samples used by processing industries in the northern region of paraná state, brazil.based on fungal and fumonisin contamination of 870 freshly harvested samples, the quality of corn used by processing industries in the northern region of parana state, brazil (2003 and 2004 crop-year) was evaluated. sampling was carried out for each crop at two points in the production chain, i.e. at reception by the processors and at the pre-drying step. corn samples were more frequently contaminated with fusarium sp. (100%) and penicillium sp. (84.1-95.3%) than aspergillus sp. (5.6-19.8%). fum ...200819680847
studies in the physiology of fusaria. the respiratory and fermentative mechanisms.1.fusarium tricothecoides was selected for a study of the respiratory and fermentative activities of fusaria. "resting cell" suspensions were investigated by the barcroft manometric technique. 2. the results of the investigation indicate clearly that the mechanism of endogenous metabolism (respiration) is distinct from the exogenous mechanism (fermentation). anaerobically no significant co(2) production is apparent without added substrate. in the presence of glucose the anaerobic co(2) evolution ...194119873241
disseminated fusarium infection with brain abscesses in a lung transplant recipient.to detail a case of disseminated fusarium infection in an immunocompromised adult. fusarium sp. infections are an important cause of fungal keratitis in immunocompetent patients and a recent outbreak related to use of a specific type of contact lens solution made national and international newspaper headlines. while reports of disseminated infection in immunosuppressed patients have appeared over the last 30 years, few have emphasized the neuropathological aspects of this emerging pathogen.200919919815
antimicrobial compounds from the endophytic fungus fusarium sp. ppf4 isolated from the medicinal plant paris polyphylla var. yunnanensis.two sterols and one fatty acid were obtained by bioassay-guided fractionation from the light petroleum extract of the fungus fusarium sp. ppf4 isolated from the rhizomes of paris polyphylla var. yunnanensis hand.-mazz., a medicinal species distributed in the southwest of china. the compounds were elucidated as 5alpha, 8alpha-epidioxyergosta-6, 22-dien-3beta-ol (1), ergosta-8(9), 22-dien-3beta, 5alpha, 6beta, 7alpha-tetraol (2), and butanedioic acid (3), respectively, by means of physical and spe ...200919967972
a new anthraquinone derivative from the marine endophytic fungus fusarium sp. (no. b77).a new anthraquinone compound, 5-acetyl-2-methoxy-1,4,6-trihydroxy-anthraquinone (1), together with four known naphthoquinones (2-5), were isolated from the culture of the mangrove fungus (no. b77). all the isolated compounds (1-5) and the acetyl derivative (1a) were established by comprehensive analysis of the spectral data, especially 1d- and 2d-nmr (hmqc and hmbc) spectra. in the primary bioassays, compound 3 showed strong inhibitory effects on the growth of hepg2 and hep2 cells, with ic(50) v ...201020013477
identification of binding proteins of fusarielin a as actin and tubulin.fusarielin a (fsa), isolated from culture of fusarium sp. k432, has anti-fungal/anti-angiogenic activity. based on our previous structure-activity relationship studies, we prepared fsa-immobilized affinity gel. the results of affinity chromatography with this gel indicated that tubulin and actin are fsa-binding proteins. the binding affinities of tubulin and actin for fsa were estimated by the use of surface plasmon resonance spectroscopy.201020045982
airborne fungi in dwellings for dairy cows and laying hens.the air of animal dwellings can contain great amounts of bioaerosol composed of dust, bacteria, fungi, and endotoxins. the composition may depend on animal species, building construction, animal accommodation, and microclimate parameters, to name just a few factors. pathogens contained may be a serious threat to animal and human health.the aim of our study was to analyse the fungi aerosol content in a stable housing dairy cows and in a coop for laying hens over the three autumn months of 2007. t ...200920061239
onychomycosis insensitive to systemic terbinafine and azole treatments reveals non-dermatophyte moulds as infectious agents.dermatophytes are the main cause of onychomycoses, but various non-dermatophyte filamentous fungi are often isolated from abnormal nails.201020110639
epidemiology and etiologic diagnosis of infectious keratitis in uberlandia, brazil.to study risk factors, microbiological characteristics, and clinical course of nonviral infectious keratitis diagnosed in a referral service of ophthalmology.201020175055
[7alpha-hydroxylation of steroid 5-olefins by mold fungi].hydroxylation activity of the mold fungi belonging to the orders dothideales, hypocreales, and mucorales towards delta(5)-3beta-hydroxysteroids was studied. the fungi bipolaris sorokiniana, fusarium sp., and rhizopus nigricans were able to introduce hydroxy group at position 7alpha; however, this ability was detected only at a low substrate load and with a low yield. a 7alpha-hydroxylase activity of the curvularia lunata vkpm f-981 culture was shown for the first time. it was demonstrated that t ...201020198922
new insights in trichoderma harzianum antagonism of fungal plant pathogens by secreted protein analysis.trichoderma harzianum all42 were capable of overgrowing and degrading rhizoctonia solani and macrophomina phaseolina mycelia, coiling around the hyphae with formation of apressoria and hook-like structures. hyphae of t. harzianum all42 did not show any coiling around fusarium sp. hyphae suggesting that mycoparasitism may be different among the plant pathogens. in this study, a secretome analysis was used to identify some extracellular proteins secreted by t. harzianum all42 after growth on cell ...201020213103
in vitro efficacy of antifungal treatment using riboflavin/uv-a (365 nm) combination and amphotericin b.to demonstrate the antimicrobial properties of riboflavin/uv-a (365 nm) against fungal pathogens.201020335618
antifungal activity of trichothecenes from fusarium sp. against clinical isolates of paracoccidioides brasiliensis.summary paracoccidioidomycosis (pcm), a human mycosis caused by the dimorphic fungus paracoccidioides brasiliensis, is a serious public health problem in several countries of latin america. in our search we found that the crude extract of the endophytic fungus ufmgcb 551 was able to inhibit several clinical strains of p. brasiliensis, and was also active in the bioautographic assay against cladosporium sphaerospermum. the endophytic fungus ufmgcb 551 was isolated from the plant piptadenia adiant ...201020337937
purification and properties of cysteine protease from rhizomes of curcuma longa (linn.).turmeric rhizome (curcuma domestica linn.) contains proteases and has proteolytic activity. curcumin from turmeric rhizomes has been used for healing many ailments, including cancer. the purpose of this study was to purify turmeric protease and to research their biochemical characteristics [corrected].201020355018
rapd-pcr analysis of genetic variation among isolates of fusarium graminearum and fusarium culmorum from wheat in adana turkey.the aim of the current study was to estimate the genetic diversity of f. graminearum and f. culmorum isolated from wheat in adana province of turkey by investigating by rapd-pcr polymorphisms. fusarium sp. affecting wheat crops were surveyed for 2 years during growing seasons in adana, turkey. thirty two isolates of fusarium were isolated from seeds and basal stem nodes of wheat displaying disease symptoms. these isolates were identified as f. avenecaum, f. culmorum, f. graminearum and f. croock ...201020437703
pathogenicity of fusarium isolates to striga hermonthica in burkina faso.striga hermonthica (del.) benth. is an important constraint to cereal crop production in burkina faso, of which sorghum (sorghum bicolor l. moench) is the most important component. native fusarium species to use as bio-control agents to s. hermonthica has been investigated. fifty one fusarium isolates obtained from diseased plants of s. hermonthica were evaluated for their pathogenicity against striga under controlled environmental conditions. of 51 fusarium isolates, 14 were pathogenic to s. he ...201020464941
in situ hybridization for coccidioides immitis 5.8s ribosomal rna sequences in formalin-fixed, paraffin-embedded pulmonary specimens using a locked nucleic acid probe: a rapid means for identification in tissue sections.coccidioides immitis/coccidioides posadasii are common causes of pulmonary infection in certain geographic areas, and are highly infectious when working with culture isolates in the laboratory. rapid techniques to accurately identify this pathogen in tissues may be of benefit for diagnosis and in limiting the exposure of laboratory personnel to this agent. locked nucleic acids (lna) are modified nucleotides in which a ribonucleoside is linked between the 2'-oxygen and the 4'-carbon atoms with a ...201020502187
unsuccessful therapy of combined mycotic infection in a severely burned patient: a case study.patients with burn trauma are always in danger of contracting an infection. although invasive mycotic infections are not as frequent as bacterial infections, high mortality and in many cases difficult diagnostics pose a serious threat not only for neutropenic patients. in more extensive burns the status is further complicated by specifically compromised immunity. the most frequent species of micromycetes isolated in burn patients are candida spp. and aspergillus spp. zygomycetes represents a rel ...200920514893
cu(ii): a "signaling molecule" of the mangrove endophyte fusarium oxysporum zzf51?we previously reported the isolation of cu-fusaric acid (cu-fa) complex from the mangrove endophyte fusarium oxysporum zzf51. in this study, we explored the mechanism of cu-fa production in the strain zzf51 by comparing with that of another endophyte fusarium sp. b2, which produced fa but not cu-fa in the same culture condition. the results allowed us to hypothesize that cu(2+) may act as a "signaling molecule" to awaken the silent fa biosynthetic genes in zzf51, inducing intracellular productio ...201020526798
the fusarium mycotoxins enniatins and beauvericin cause mitochondrial dysfunction by affecting the mitochondrial volume regulation, oxidative phosphorylation and ion homeostasis.the mechanisms of cell toxicity of mycotoxins of the enniatin family produced by fusarium sp. enniatin b, a mixture of enniatin homologues (3% a, 20% a(1), 19% b, 54% b1) and beauvericin, were investigated. in isolated rat liver mitochondria, exposure to submicromolar concentrations of the enniatin mycotoxins depleted the mitochondrial transmembrane potential, uncoupled oxidative phosphorylation, induced mitochondrial swelling and decreased calcium retention capacity of the mitochondria. the mit ...201020621153
development of a new mlst scheme for differentiation of fusarium solani species complex (fssc) isolates.fungi belonging to the fusarium solani species complex (fssc) are well known plant pathogens. in addition to being the causative agent of some superficial infections, fssc has recently emerged as a group of common opportunistic moulds, mainly in patients with haematological malignancies. molecular typing methods are essential in order to better understand the epidemiology of such opportunistic agents with the final goal of preventing contamination. a three-locus typing scheme has thus been devel ...201020624428
diversity and antimicrobial activity of endophytic fungi associated with the alpine plant saussurea involucrata.endophytic fungi are rich in species diversity and may play an important role in the fitness of their host plants. this study investigated the diversity and antimicrobial potential of endophytic fungi obtained from saussurea involucrata kar. et kir. a total of 49 endophytic fungi were isolated from s. involucrata and identified using morphological and molecular techniques. extracts of fermentation broth from the 49 fungi were tested for antimicrobial activity against pathogenic microorganisms us ...201020686222
hypersensitivity pneumonitis due to molds in a saxophone player.this 48-year-old patient was evaluated for an interstitial pneumonia. an open-lung biopsy showed a pattern of nonspecific interstitial pneumonia. the ct scan appearance, showing mosaic ground-glass opacities in the ventilated parts of the lung, the centrolobular predominance of inflammation on the lung sections, and the presence of a lymphocytic alveolitis at bal suggested a hypersensitivity pneumonitis. the patient was a white-collar worker and had no contact with pets, birds, drugs, or molds a ...201020822994
diversity and screening for antimicrobial activity of endophytic fungi from alstonia scholaris.endophytic fungi of three tissues (petiole, bark and leaf) of alstonia scholaris were assessed. a total number of 1,152 endophytic fungi were isolated from 1,002 different plant segments of seven different localities of paschim medinipur, west bengal, india. the isolated fungi belong to nineteen genera, including four unidentified fungi and yeast. colletotrichum sp. (20.39%) and sordaria sp. (29.68%) were most commonly isolated from this plant. hyalopus sp., fusarium sp. and curvularia sp. were ...201020870593
epidemiological profile of fungal keratitis in urban population of west bengal, india.corneal diseases are one of the major causes of visual loss and blindness, second only to cataract. amongst corneal diseases, microbial keratitis is a major blinding disease. in some countries, fungal keratitis accounts for almost 50% of patients with culture-proven microbial keratitis.200920927207
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