| brain angiotensin: critical role in the ongoing regulation of body fluid homeostasis and cardiovascular function. | the existence of an endogenous brain-angiotensin system and its association with cardiovascular and body water regulation has been recognized for over a decade. nevertheless, the importance of the brain-angiotensin system to the instantaneous regulation of these processes has not been firmly established. a 5-minute intracerebroventricular (icv) infusion of the angiotensin antagonist, [sar1,thr8]-aii, effectively lowered the blood pressure in normotensive rats. additionally, application of the se ... | 1989 | 2755868 |
| [infection caused by paracoccidioides brasiliensis in people living in cocle and veraguas, republic of panamá]. | intradermal application of yeast phase paracoccidioidin antigen obtained by sonication and lysis from the fungus to 111 residents of veraguas province (68 men and 43 women) and 130 cocle province residents (57 men and 73 women) elicited a positive reaction in 9.01% (1/111) and 26.15% (34/30) of the veraguas and cocle residents respectively. positive reactions ranged between papules of 5 and 27 mm in diameter, the majority being between 11 and 20 mm. infection was more frequent in men (1:1.47 for ... | 1989 | 2762594 |
| developmental alternations in offspring of female rats orally intoxicated by aluminum chloride or lactate during gestation. | the oral treatment of pregnant rats by aluminum chloride or lactate at various doses was applied from day 1 to day 21 of gestation to determine its influence on mortality, weight evolution, and neuromotor maturation of their pups. no effect of treatment on litter size was detected, but an increased mortality appeared during the first week: treatment by aluminum lactate was less active than was an equivalent treatment by aluminum chloride. weight was transitorily delayed, but the reversal of this ... | 1989 | 2763207 |
| treatment of childhood bacterial meningitis with ceftriaxone once daily: open, prospective, randomized, comparative study of short-course versus standard-length therapy. | fifty-two children were included in this study to evaluate and compare short- versus standard-length ceftriaxone therapy for bacterial meningitis. the duration of the short-course regimens was 4, 6 and 7 days for neisseria meningitidis, hemophilus influenzae and streptococcus pneumoniae, respectively. the standard-length regimens were twice as long. on the basis of a computer-generated randomization list, 26 children were assigned either to the short- or to the standard-treatment regimen. ceftri ... | 1989 | 2766869 |
| combined gastric pull-up and microvascular jejunal transfer procedure after pharyngolaryngoesophagectomy. | a case of simultaneous carcinomas of the lower thoracic esophagus and the oropharynx in a 62-year-old man is reported. reconstruction was successfully performed using a one-stage operation consisting of pharyngolaryngoesophagectomy followed by a combination of gastric pull-up and microvascular jejunal transfer procedures. the postoperative course was uneventful except for regurgitation of food, which persisted for 1 month. | 1989 | 2774731 |
| ductus diverticulum aneurysm and coronary stenosis: repair using circulatory arrest. | the case of a 68-year-old man with advanced symptomatic coronary artery disease and large aneurysm of the diverticulum of the ductus arteriosus is presented. both the coronary artery disease and the aneurysm were successfully repaired at the same time via median sternotomy using cardiopulmonary bypass and a brief period of profound hypothermic circulatory arrest. to our knowledge, this is the 27th reported case of such an aneurysm in an adult. | 1989 | 2774734 |
| smokeless tobacco use and oral epithelial dysplasia. | one hundred eight patients with a histologically confirmed diagnosis of epithelial dysplasia of the oral cavity and a prior history of smokeless tobacco (st) usage were identified. the mean age at time of diagnosis for female patients was almost a decade later than for male patients (63.6 versus 51.2 years). in addition, women were more likely (p = 0.02) to have moderate or severe epithelial dysplasia than men. the two most common locations (buccal mucosa/vestibule and alveolar ridge/gingiva) ac ... | 1989 | 2776112 |
| electron microscopic study of subcutaneous and intraperitoneal splenules in the mouse. | the development of splenules derived from slices of freshly removed autologous spleen implanted subcutaneously or intraperitoneally was followed by light and electron microscopy from day 2 to day 70. within 48 hr after transplantation, a rough space filled with blood, unlined by endothelium, formed just under the surface of the splenic fragment. the tissue central to this vascular space was disrupted and necrotic. in the outer portion of the vascular space, fibroblasts appeared and created locul ... | 1989 | 2782279 |
| first-pass uptake of verapamil, diazepam, and thiopental in the human lung. | the first-pass uptake of verapamil, diazepam, and thiopental in the human lung was determined using multiple-indicator dilution techniques. these three drugs represent lipid-soluble agents that differ in their ionic characteristics at physiological ph. verapamil, a basic lipophilic amine, underwent significant uptake, with 50% of the drug accumulating in lung tissue during the first pass. with diazepam, a nonbasic lipophilic amine, there was 30% uptake during the first pass through the human lun ... | 1989 | 2782646 |
| [changes in the age of illness onset in cancer of the hypopharynx and larynx since 1960. the epidemiologic context in austria]. | between 1960 and 1987, 1567 laryngeal and hypopharyngeal carcinomas were diagnosed at the first ent clinic of vienna university. patients under 50 years of age increased steadily in number and proportion. three subgroups of carcinomas were considered: glottic (including subglottic), supraglottic and hypopharyngeal. a total of 1442 cases could be studied in detail. in the supraglottic and hypopharyngeal groups the proportion under 50 years of age rose significantly, whereas in the glottic cases n ... | 1989 | 2785098 |
| human fetal dopamine neurons grafted into the striatum in two patients with severe parkinson's disease. a detailed account of methodology and a 6-month follow-up. | by using stereotaxic surgical techniques, ventral mesencephalic tissues from aborted human fetuses of 8 to 10 weeks' gestational age were implanted unilaterally into the striata in two patients with advanced parkinson's disease. the patients were treated with a cyclosporine, azathioprine, and steroid regimen to minimize the risk for graft rejection. they were examined for 6 months preoperatively and 6 months postoperatively and continued to receive the same doses of antiparkinsonian medication. ... | 1989 | 2786405 |
| in-vitro activity of a new penem fce 22101. | the in-vitro activity of a new penem fce 22101 was evaluated in comparison with other antimicrobial agents against 966 aerobic and anaerobic gram-negative and gram-positive bacteria. fce 22101 inhibited 90% of enterobacteriaceae, haemophilus influenzae, h. parainfluenzae, h. ducreyi, neisseria gonorrhoeae, branhamella catarrhalis, staphylococcus aureus, staph. epidermidis and group b streptococcus at concentrations of 0.12-4.0 mg/l. pseudomonas aeruginosa was resistant with mic90 of greater than ... | 1989 | 2786518 |
| the effect of thiamin supplementation on the thiamin status of turkey poults with ethanol-induced cardiomegaly. | 1. turkey poults were treated with graded doses (0-5% v/v) of ethanol for 6 weeks. some birds were concomitantly injected intramuscularly with thiamin. 2. erythrocyte transketolase (tk) activity was significantly decreased and the erythrocyte pyrophosphate (tpp) effect and blood concentrations of lactate and pyruvate were statistically greater in all ethanol-treated poults by 42 days. 3. thiamin restored tk activity to normal levels and decreased the tpp effect and blood concentrations of lactat ... | 1987 | 2820834 |
| do l3t4+ t cells act as effector cells in protection against influenza virus infection. | this study aimed to analyse the roles of lyt 2+ and l3t4+ memory t-cell subpopulations in murine influenza infection. previous work has shown that lyt 2+ cytotoxic t-cell (tc) clones can adoptively transfer protection. we therefore wished to see whether l3t4+ (th) cells could also act as protective effector cells. donors for adoptive cell transfer were thymectomized mice, depleted in vivo of either lyt 2+ or l3t4+ t cells with monoclonal antibodies (mab) and then infected with influenza virus (a ... | 1987 | 2820868 |
| do l3t4+ t cells act as effector cells in protection against influenza virus infection. | this study aimed to analyse the roles of lyt 2+ and l3t4+ memory t-cell subpopulations in murine influenza infection. previous work has shown that lyt 2+ cytotoxic t-cell (tc) clones can adoptively transfer protection. we therefore wished to see whether l3t4+ (th) cells could also act as protective effector cells. donors for adoptive cell transfer were thymectomized mice, depleted in vivo of either lyt 2+ or l3t4+ t cells with monoclonal antibodies (mab) and then infected with influenza virus (a ... | 1987 | 2820868 |
| 5'-nucleotidase in rat liver lysosomes. | 5'-nucleotidase was found in purified rat liver tritosomes. when tritosomes were subfractionated into the membrane and soluble contents fractions, 73% of the total 5'-nucleotidase activity was found in the membrane fraction and 24% in the soluble contents fraction. immunoblotting using specific polyclonal antibodies against the rat liver plasma membrane 5'-nucleotidase showed that the mobilities on sds-polyacrylamide gel electrophoresis of both 5'-nucleotidases in the membrane and contents fract ... | 1987 | 2820949 |
| 5'-nucleotidase in rat liver lysosomes. | 5'-nucleotidase was found in purified rat liver tritosomes. when tritosomes were subfractionated into the membrane and soluble contents fractions, 73% of the total 5'-nucleotidase activity was found in the membrane fraction and 24% in the soluble contents fraction. immunoblotting using specific polyclonal antibodies against the rat liver plasma membrane 5'-nucleotidase showed that the mobilities on sds-polyacrylamide gel electrophoresis of both 5'-nucleotidases in the membrane and contents fract ... | 1987 | 2820949 |
| effects of palindrome size and sequence on genetic stability in the bacteriophage phi x174 genome. | by inserting palindromes of varying length and sequence into a non-essential region of the bacteriophage phi x174 genome we have investigated the effect of palindrome size and sequence on their genetic stability. multimers of increasing size of the ecori linker ccgaattcgg (e), the bamhi linker ccggatccgg (b) or mixtures of both (e, b) were inserted into the pvuii site of a previously constructed bacteriophage strain phi x174 j-f ins6. the largest inserts that could be maintained in the genome wi ... | 1987 | 2824789 |
| mucosal antibody response of calves after oral and intrabronchial administration of rotavirus. | | 1987 | 2827448 |
| the kappa agonists pd117302 and u50488 produce a biphasic effect on 24 hour food intake in the rat. | the effects of the selective kappa opioid receptor agonists pd117302 [(+/-)trans-n-methyl-n-[2-(1-pyrrolidinyl)-cyclohexyl-]benzo-[b]- thiophene-4-acetamide] and u50488 (trans-3,4-dichloro-n-methyl-n-[2-(1-pyrrolidinyl)-cyclohexyl]- benzeneacetamide) were investigated on food intake in non-deprived rats over a 24 hr period following subcutaneous administration. at doses of 2.5 mg/kg and 5 mg/kg, both pd117302 and u50488 initially increased food intake. between 6-24 hr, however, pd117302 (2.5-10 ... | 1987 | 2829048 |
| investigation of opioid receptor binding protein in adult and fetal mouse brain membranes by photoaffinity labeling. | 125i-[d-ala2,p-n3-phe4-met5]enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. the photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. a 46,000-da protein was specifically photo-labeled in the adult mouse brain membranes. the photolabeling of this protein was inhibited in the presence of [d-ala2,met5]enkephalin and a peptide ligand specific for the mu-opioid receptor. no specific ... | 1988 | 2829063 |
| treatment of cytomegalovirus infections in renal transplant recipients with 9-(1,3-dihydroxy-2-propoxymethyl) guanine. | | 1988 | 2837848 |
| modification of radiation induced changes in splenic weight of mouse by s-2 (3-aminopropylamino)-ethyl phosphorothioic acid. | | 1988 | 2838866 |
| a one-step preparative method for separating ser 6-phosphorylated hmg 14 from unphosphorylated hmg 14 and in vitro phosphorylation reaction components. | while clear evidence exists for the regulation of the phosphorylation of the very basic high mobility group (hmg) and histone chromatin proteins, the physiological role of their phosphorylation remains poorly understood. elucidation of these roles has been difficult, in part, because of the inability to obtain sufficient quantities of purified phosphorylated derivatives. we have used mono s cation-exchange chromatography to prepare milligram quantities of pure ser 6-phosphorylated hmg 14 (ser 6- ... | 1988 | 2839054 |
| the potassium channel opening action of pinacidil; studies using biochemical, ion flux and microelectrode techniques. | in rat aorta and rat portal vein, (-)- and (+)-pinacidil each produced a concentration-dependent inhibition of tension development. although the (-) isomer was the more potent, concentration effect curves for each isomer were steep with similar slopes. in rat portal vein, tetraethylammonium and procaine antagonised the relaxant effect of (+/-)-pinacidil, whereas 3,4-diamino-pyridine was without effect. intracellular microelectrode recording in rat portal vein showed that low concentrations of (+ ... | 1988 | 2848206 |
| restoration of torque in defective flagellar motors. | paralyzed motors of mota and motb point and deletion mutants of escherichia coli were repaired by synthesis of wild-type protein. as found earlier with a point mutant of motb, torque was restored in a series of equally spaced steps. the size of the steps was the same for both mota and motb. motors with one torque generator spent more time spinning counterclockwise than did motors with two or more generators. in deletion mutants, stepwise decreases in torque, rare in point mutants, were common. s ... | 1988 | 2849208 |
| a murine monoclonal antibody raised against human non-small cell carcinoma of the lung. | murine monoclonal antibodies (mabs) reactive with human non-small cell carcinoma of the lung (nsccl) were produced following immunization with a membrane preparation of calu-3, a human adenocarcinoma of the lung cell line grown in nude mice. positive hybrids unreactive with normal liver membranes and human peripheral white blood cells were selected for further testing. one mab (rs5-4h6) recognized an antigen expressed in a variety of lung and other carcinoma cell lines as detected by flow cytome ... | 1988 | 2853133 |
| idazoxan: a novel xylazine antagonist. | | 1985 | 2864759 |
| zinc absorption in inflammatory bowel disease. | zinc absorption was measured in 29 patients with inflammatory bowel disease and a wide spectrum of disease activity to determine its relationship to disease activity, general nutritional state, and zinc status. patients with severe disease requiring either supplementary oral or parenteral nutrition were excluded. the mean 65zncl2 absorption, in the patients, determined using a 65zn and 51cr stool-counting test, 45 +/- 17% (sd), was significantly lower than the values, 54 +/- 16%, in 30 healthy c ... | 1986 | 2873002 |
| effects of alpha and beta adrenolytic agents on the changes in arterial pressure induced by intrahypothalamic injection of carbachol and nicotine. | in order to elucidate some aspects of cholinergic and adrenergic interaction in the dorsomedial nucleus of the hypothalamus and its effect on systemic arterial pressure, carbachol and nicotine were injected into this area alone or preceded by regitine and propranolol. both carbachol and nicotine induced hypotensive effects in normotensive anesthetized rats. previous application of 20, 40, 80 and 160 nmol propranolol did not alter the hypotension induced, but doses of 20, 40 and 80 nmol regitine ... | 1986 | 2873596 |
| capsaicin-induced beta-adrenergic action on energy metabolism in rats: influence of capsaicin on oxygen consumption, the respiratory quotient, and substrate utilization. | the mode of action of capsaicin on energy metabolism was investigated in rats. the oxygen consumption was higher when capsaicin (6.0 mg/kg) was intraperitoneally injected than when it was not injected. the respiratory quotient (r.q.) increased and then decreased after the administration of capsaicin. the levels of serum glucose and immunoreactive insulin rapidly increased after the administration of capsaicin. also, liver glycogen rapidly decreased, in contrast to the serum glucose concentration ... | 1986 | 2876434 |
| autonomic changes elicited by chemical stimulation of mediodorsal nucleus of the thalamus. | chronic indwelling cannulas were implanted in the mediodorsal nucleus (md) of the thalamus in new zealand albino rabbits. heart rate (hr), blood pressure (bp), respiration and electromyographic activity (emg) were recorded subsequent to the injection of saline, l-glutamate, or carbachol through the previously implanted cannulas. dose related decreases in hr and increases in bp were obtained after administration of both l-glutamate and carbachol at doses in the micromolar range. control injection ... | 1986 | 2876440 |
| interactions between vecuronium and suxamethonium in the dog. | the non-depolarising muscle relaxant vecuronium (0.2 mg kg-1) was administered to four dogs. at 50 per cent return of neuromuscular activity, as measured by the train-of-four technique, the depolarising muscle relaxant suxamethonium (0.3 mg kg-1) was injected intravenously. at 50 per cent return of neuromuscular activity reversal of the block was achieved with atropine and neostigmine. the duration of action of suxamethonium was reduced by the prior administration of vecuronium. in the second se ... | 1986 | 2876469 |
| modification of hiv competitive assay to reduce frequency of false negative results. | | 1987 | 2889060 |
| fecal colonization with p-fimbriated escherichia coli in newborn children and relation to development of extraintestinal e. coli infections. | the incidence of e. coli pyelonephritis before the age of one year among the children born at danderyd hospital during a ten year period was studied. during the study period, 4 or 5 outbreaks of e. coli pyelonephritis occurred among the children who had previously been staying in the hospital's neonatal ward. these outbreaks seemed to have been caused by nosocomial spread of and fecal colonization with certain virulent e. coli strains among the children staying in the ward during certain periods ... | 1987 | 2889317 |
| a mixed endocrine adrenal tumour causing steatorrhoea. | a 60 year old man developed steatorrhoea, weight loss, mild diabetes mellitus, labile hypertension and limb cramps. raised plasma concentrations of catecholamines, particularly noradrenaline and a computed tomography-scan showing an adrenal tumour strongly suggested a pheochromocytoma. adrenoreceptor blockade reversed the symptoms, decreased faecal fat, and increased duodenal trypsin to normal concentrations. after adrenalectomy the patient was asymptomatic and there was no steatorrhoea. the blo ... | 1987 | 2890560 |
| a fraction enriched in dendrodendritic synaptosomes isolated from rat olfactory bulb: morphology and transmitter release. | a fraction enriched in dendro-dendritic synaptosomes was isolated from rat olfactory bulb by a rapid method. synaptosomes preserved their ultrastructure and showed configurational changes in relation to incubation in physiological ion medium as described earlier in the case of cortical synaptosomes. dendro-dendritic synaptosomes were larger and contained more mitochondria than cortical synaptosomes. doublets of terminals synapsing with each other were frequently seen and each terminal contained ... | 1986 | 2891058 |
| the zonation of liver and the distribution of fructose 2,6-bisphosphate in rat liver. | livers of starved rats refed for 2 h were perfused in situ by a modification of the dual digitonin pulse technique of quistorff and grunnet (quistorff, b., and grunnet, n. (1987) biochem. j. 243, 87-95). a pulse of digitonin (2 mg/ml) was infused first antegrade through the portal vein followed retrograde through the vena cava, or in reverse order, 13 mg of digitonin per zone. microscopic examination showed that this procedure permeabilized the periportal and perivenous zones of the liver withou ... | 1988 | 2895107 |
| stabilization and quantitative analysis of fecapentaenes in human feces, using synthetic fecapentaene-12. | naturally occurring fecal mutagens, called fecapentaenes, are hypothesized to contribute to large bowel carcinogenesis. to understand health risks it is necessary to relate fecal mutagen concentrations to intestinal pathologies, i.e. colon cancer. however, fast and reliable methods for analysis of stool for fecapentaene levels are not available. this study presents an evaluation of stabilizing effects on synthetic fecapentaene-12 of various antioxidants, indicating tri-ethylamine to give the bes ... | 1989 | 2917340 |
| carbon-13 in natural cholesterol. | in order to assess the feasibility of using stable 13c as a tracer for studying cholesterol metabolism in vivo the 13c content of some commonly consumed foods and the stability of the 13c content of human serum cholesterol has been investigated. a procedure is described for the extraction and purification of cholesterol from food, tissues and serum suitable for 13c analysis by mass spectrometry. the method caused no significant isotope fractionation effects. generally, there was very little diff ... | 1986 | 2937477 |
| dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility. | in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ... | 1986 | 2942039 |
| rejection of skin grafts and generation of cytotoxic t cells by mice depleted of l3t4+ cells. | in mice, "helper/inducer" t cells can be depleted by treatment with a rat monoclonal antibody to the cell surface antigen, l3t4, which is homologous to the human antigen t4 (cd4). in order to examine the contribution of "helper/inducer" t cells to cellular immunity, c57bl/6 (h-2b) mice were treated weekly with 1 mg i.v. of a monoclonal antibody to l3t4. three days after the first injection, the mice received skin grafts from balb/c (h-2d) mice. the mice were then examined for skin graft rejectio ... | 1986 | 2947360 |
| structural and metabolic correlates of cell injury in the hypertrophied myocardium during valve replacement. | to characterize the ultrastructural and metabolic changes occurring in the hypertrophied ventricle during cardiac operations in man, we studied 36 patients with valvular heart disease undergoing valve replacement, during which multiple doses of cold potassium cardioplegic solution were administered (group i). each patient had substantial ventricular hypertrophy according to measurements made of left ventricular mass, with a mean of 232.1 +/- 19.8 gm/m2 (normal: 92 +/- 16 gm/m2). serial biopsy sp ... | 1987 | 2952840 |
| use of acupuncture for the treatment of chronic back pain in horses: stimulation of acupuncture points with saline solution injections. | fifteen horses that could not train or perform their sport at an acceptable level because of chronic back pain of 2 to 24 months' duration, and that did not obtain lasting improvement from other previous treatments, were treated with acupuncture. the treatments consisted of injection of 1 ml of sterile saline (0.9% nacl) solution, with no preservatives, at 9 acupuncture points. the horses were treated once a week for a mean of 9 treatments. the mean number of treatments before obvious improvemen ... | 1987 | 2953702 |
| an immunohistological study of cd4+ lymphocyte subsets within inflammatory lesions with special reference to rheumatoid arthritis and inflammatory bowel disease. | the monoclonal antibodies (mabs) wr16, uchl1 and wr19 identify subsets of cd4+ lymphocytes that have been functionally characterized as suppressor inducer cells or helper inducer cells. these were applied as components of a panel of lymphocyte-specific mabs for the phenotypic analysis of lymphocyte populations within biopsies taken from rheumatoid synovial membrane and normal and inflamed gut. the phenotype of peripheral blood lymphocytes from patients with rheumatoid arthritis were also compare ... | 1988 | 2974833 |
| coordinate regulation of the levels of type iii and type i collagen mrna in most but not all mouse fibroblasts. | a mouse genomic clone was isolated by cross-hybridization with a dna fragment which codes for the nh2-propeptide of chick alpha1(iii) collagen. the region of cross-hybridization within the mouse clone was localized, its sequence determined, and an exon coding for the nh2-propeptide of mouse alpha1(iii) collagen was identified. this dna fragment hybridizes to an rna species of approximately 5300 nucleotides, slightly larger than the major alpha2(i) collagen rna species. the mouse type iii collage ... | 1985 | 2981217 |
| [mechanism of ischemic disorders of fatty acid oxidation in heart mitochondria]. | the article deals with the effect of permanent, temporal and total myocardial ischemia on the oxidation of acetate, hexanoate, palmitoylcarnitine and palmitoyl-coa in isolated rabbit heart mitochondria. all the three models of ischemia in different experimental situations demonstrated a similar degree of fatty acid oxidation suppression independent of the length of acyl residue, the suppression being the greatest during the first hours and in total ischemia. both in the control and in ischemia, ... | 1985 | 2988585 |
| [mechanism of action of fentolamin on the gastric secretion]. | thirty-three experiments were made on mongrel dogs operated on according to basov, with the use of phentolamine, pilocarpine, atropine, histamine and their combinations. it was established that administration of histamine gives rise to an increase in gastric secretion, provided the animals are pretreated with phentolamine or with combined pilocarpine and phentolamine. histamine-induced gastric secretion considerably decreases, provided the animals are pretreated with atropine and ceases almost c ... | 1985 | 2993020 |
| [cytomegaloviruses in sperm and the risk of sexual transmission]. | | 1985 | 2996789 |
| the epidemiology of the acquired immunodeficiency syndrome. | | 1986 | 3008169 |
| preparation of the fhua (tona) receptor protein from cell envelopes of an overproducing strain of escherichia coli k-12. | a rapid and simple method for purification of the fhua receptor protein from cell envelopes of a fhua-overproducing strain of escherichia coli k-12 was developed. the overproduction of fhua was programmed by the thermoamplifiable plasmid phk232, which carried the fhuacd genes of plc19-19 of the clarke and carbon collection. at low temperature (27 degrees c), phk232 specified the overproduction of fhua to levels comparable to those of major outer membrane proteins ompf, ompc, and ompa. the amount ... | 1986 | 3009396 |
| peptidoglycan synthetic activities in membranes of escherichia coli caused by overproduction of penicillin-binding protein 2 and roda protein. | penicillin-binding protein (pbp)-2 and the roda protein are known to function in determining the rod shape of escherichia coli cells. peptidoglycan biosynthetic reactions that required these two proteins were demonstrated in the membrane fraction prepared from an e. coli strain that overproduced both of these two proteins and which lacked pbp-1b activity (the major peptidoglycan synthetase activity in the normal e. coli membranes). the cross-linked peptidoglycan was synthesized from udp-n-acetyl ... | 1986 | 3009484 |
| [differential diagnosis of disseminated periventricular calcification]. | | 1986 | 3020717 |
| [differential diagnosis of disseminated periventricular calcification]. | | 1986 | 3020717 |
| selective radioligands for characterization and neuroanatomical distribution studies of brain opioid receptors. | | 1986 | 3025736 |
| selective radioligands for characterization and neuroanatomical distribution studies of brain opioid receptors. | | 1986 | 3025736 |
| structurally related bacillus thuringiensis delta-endotoxins display major differences in insecticidal activity in vivo and in vitro. | many strains within the 22 serotypes of bacillus thuringiensis produce crystal delta-endotoxins with slight differences in their insecticidal toxicity spectrum in vivo. since the basis of this specificity is unknown, we chose to compare the activity of delta-endotoxins from three strains: b. thuringiensis var. kurstaki hd-1, var. aizawai hd-249 and var. thuringiensis hd-350, both in vivo and on insect cell lines in vitro. immunoblotting with antisera to activated var. kurstaki p1 lepidopteran to ... | 1986 | 3027111 |
| diabetic embryopathy and fuel-mediated organ teratogenesis: lessons from animal models. | the growing recognition that faulty maternal metabolism during early organogenesis may be implicated in the increased incidence of birth defects in pregnancies complicated by diabetes has prompted worldwide efforts to institute improved preconceptional metabolic regulation. however, the failure to identify the periods of greatest risk for diabetic embryopathy, the mediating teratogen(s), and the underlying mechanisms have complicated attempts to establish precise therapeutic guidelines and targe ... | 1988 | 3053387 |
| stimulation of escherichia coli division by low-intensity monochromatic visible light. | | 1988 | 3068688 |
| agent-specific ras oncogene activation in rat thyroid tumours. | activated ras oncogenes have been detected in a number of rodent tumour model systems (balmain et al., 1986; barbacid 1987), in which chemical carcinogens have been the most widely used inducing agents. we have investigated the involvement of activated oncogenes in a single well-characterised tumour model, rat thyroid neoplasia, produced not only by chemical carcinogen (nitrosomethylurea, nmu) and by ionizing radiation but also by elevated trophic stimulation alone. we have indentified a strikin ... | 1988 | 3078959 |
| inhibitors of bacillus subtilis dna polymerase iii. influence of modifications in the pyrimidine ring of anilino- and (benzylamino)pyrimidines. | substituent effects governing inhibition of dna polymerase iii from bacillus subtilis were examined in several series of n6-substituted 6-aminopyrimidines. the presence of alkyl groups as large as n-butyl in the 3-position of 6-(5-indanylamino)uracil had no effect on inhibitor-enzyme binding. substituents in the 4-position of a series of 2-amino-6-(benzylamino)pyrimidines had complex effects: alkoxy and phenoxy derivatives were less active than the parent 4-oxo (isocytosine) compound, but alkylp ... | 1986 | 3084785 |
| multiple phosphorylation sites of rat liver glycogen synthase. | rat liver glycogen synthase was purified to homogeneity by an improved procedure that yielded enzyme almost exclusively as a polypeptide of mr 85,000. the phosphorylation of this enzyme by eight protein kinases was analyzed by cleavage of the enzyme subunit followed by mapping of the phosphopeptides using polyacrylamide gel electrophoresis in the presence of sds, reverse-phase high-performance liquid chromatography and thin-layer electrophoresis. cyclic amp-dependent protein kinase, phosphorylas ... | 1986 | 3091084 |
| ontogenetic development of pancreatic thyrotropin-releasing hormone in human foetuses and in infants. | the ontogeny of thyrotropin-releasing hormone (trh) in pancreata of human foetuses from 15-36 weeks of gestation and of infants has been studied. trh was detectable in the pancreas of a 15 week old foetus; a progressive increase of pancreatic trh content was observed until the 34th week of gestation, whereas a progressive decrease was found in the late period of pregnancy and in 1 year old infants. in contrast, the pancreatic insulin content showed a progressive increase during the entire pregna ... | 1986 | 3092527 |
| effects of vasopressin, des-glycinamide vasopressin and amphetamine on a combined passive and active avoidance task. | the effects of peripherally injected arginine vasopressin (avp), its des-glycinamide derivative (dgavp), which has limited pressor activity, and d-amphetamine (amp) were studied on a combined passive and active avoidance task (carew 1970). in this procedure, the rat experiences mild foot-shock in one of two distinctively coloured compartments, followed by drug treatment. on the next day, re-entry latency into either of the two compartments is measured (latency index), and the actual compartment ... | 1986 | 3101105 |
| further evidence supporting the concurrent influence of aflatoxin and manganese. | trace elements, including manganese may afford protection from deleterious effects of aflatoxin. young male fischer rats received ip injections of aflatoxin b1 (afb1) in dimethyl sulfoxide (dmso), 1 mg/kg, 2 mg/kg or 4 mg/kg. control groups received dmso ip or no injection. all animals were intubated with 3 microci of [54mn]-mncl2 12 hr post-injection. sacrifice occurred 72 hr after gavage of the radiolabel. all tested levels of afb1 affected the loss of total body radioactivity. this response w ... | 1987 | 3107203 |
| osteonectin content in human osteogenesis imperfecta bone shows a range similar to that of two bovine models of oi. | samples of human osteogenesis imperfecta (oi) bone were analyzed for osteonectin content by sds gel electrophoresis and immunodetection on western blots. the oi bone osteonectin content varied from normal to severely depressed. previously, we showed that two clinically identical but genetically unrelated bovine models of oi were differentiated biochemically by their bone osteonectin content: one oi model had normal bone osteonectin while the other was severely depressed in this parameter. the da ... | 1987 | 3107778 |
| a continuous spectrophotometric assay for the bacillus cereus phospholipase c using a thiophosphate substrate analog: evaluation of assay conditions and chromogenic agents. | a thiophosphate analog of dioctanoylphosphatidylcholine has been used as the substrate in a continuous spectrophotometric assay for the bacillus cereus phospholipase c. the reaction has been monitored at 412 nm using 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb) and at 324 nm using 4,4'-dithiopuridine (dtp) as the respective thiol-reactive chromogenic agents. an optimum ph 6.0 was determined for the phospholipase c-catalyzed reaction which was independent of the chromogen utilized. although the rea ... | 1987 | 3118733 |
| regulation of intestinal apolipoprotein b synthesis and secretion by caco-2 cells. lack of fatty acid effects and control by intracellular calcium ion. | to investigate the mechanism of control of intestinal apolipoprotein b (apob) secretion, we studied the effects of fatty acids and calcium ionophores on the human intestinal model cell line caco-2. although treatment with various fatty acids (18:1w9, 18:2w6, and 20:5w3) complexed to bovine serum albumin resulted in a dramatic redistribution of apob-100 from the low density and high density lipoproteins to the very low density lipoprotein fraction, there was no effect of any of the fatty acids on ... | 1988 | 3125180 |
| arachidonic acid, prostaglandin e2 and f2 alpha inhibit leucine degradation in chick skeletal muscle. | arachidonic acid (5 microm), prostaglandin e2 (0.28 microm) and f2 alpha (14 microm) inhibited (p less than 0.01) the rates of net leucine transamination, leucine oxidative decarboxylation and total co2 production from leucine in extensor digitorum communis muscles from fed ten-day-old chicks. indomethacin (50 microm) markedly inhibited (p less than 0.01) the rate of pge2 production in the presence of 5 microm arachidonic acid and prevented the inhibition of leucine degradation by arachidonic ac ... | 1988 | 3130060 |
| ethanol and its novel metabolites inhibit insulin action on adipocytes. | the effects of ethanol, acetaldehyde, and the two novel metabolites of ethanol 2,3-butanediol and 1,2-propanediol on basal and insulin-stimulated adipocyte lipogenesis and glucose oxidation in vitro were investigated. whereas ethanol and acetaldehyde inhibited both processes at concentrations far greater than those found in alcoholic subjects, the two diols were extremely potent inhibitors of basal and insulin-stimulated adipocyte metabolism at concentrations far below those observed in alcoholi ... | 1988 | 3133260 |
| colon cancer cells in peripheral blood of cancerous tamarins. | diagnosing colon cancer in its early stages would lower the mortality rate. the cotton-top tamarin, saguinus oedipus, serves as a model for the study of human colon cancer. this new world monkey has a high incidence of colitis and colon cancer. the mouse anti-human monoclonal antibody br55.2, with specificity for human colon adenocarcinoma, was biotinylated. peripheral blood mononuclear cells (pbmc) from animals with colon cancer were fluorescently stained with the biotinylated br55.2. these res ... | 1988 | 3135964 |
| effect of hypophysectomy and hormone replacement on fatty elongation in isolated microsomes of rat testes. | effect of hypophysectomy on fatty acid elongation was investigated in isolated rat testicular microsomes incubated with [14c]malonyl coa. hypophystectomy resulted in a 40% decrease in total incorporation of 14c into fatty acids of microsomes. several 18- and 20-carbon fatty acids of testicular microsomes from hypophysectomized rats had less 14c than did those from nonhypophysectomized ones, whereas only docosa-7,10,13,16-tetraenoic acid (22:4w6) had more 14c. testosterone injected subcutaneously ... | 1988 | 3142378 |
| prostacyclin modulates inducible major histocompatibility complex products expression in mouse kidney. | | 1987 | 3152693 |
| ryanodine: its possible mechanism of action in the caffeine-sensitive calcium store of smooth muscle. | the caffeine-sensitive intracellular ca store was characterized and the mechanism of action of ryanodine in the store was studied using k-depolarized guinea-pig taenia caecum. (1) after incubation of the preparation with cacl2 (ca loading), caffeine was applied in ca-deprived medium, to produce a transient contraction and to monitor the amount of the stored ca. as duration of ca deprivation was prolonged, the amplitude of the caffeine-induced contraction was decreased. when ryanodine was applied ... | 1988 | 3174395 |
| [ultrastructure of the cerebral cortex in the rat after the effect of electromagnetic impulse]. | in mature rats an area on the head has been subjected to a single radiation for 1.5 sec with microwaves in the continuous regimen of generation, frequency 2.4 ghz level of the specific absorbed power 5 w/g, that is accompanied with appearance of convulsions. under anesthesia specimens of the superficial layer of the cerebral superlateral part are taken and subjected to electron microscopical investigation. immediately after radiation and in 2 h certain disorders in microcirculation and reactive ... | 1988 | 3207391 |
| [morphologic studies of the extrahepatic bile ducts in infancy, childhood and youth]. | morphology of the extrahepatic bile ducts was examined with light microscopic methods in 55 human cases from the age of 28 gestational weeks up to 19 years. we found that barrel-shaped cells, rod-shaped cells and goblet cells are obligatory components of the bile duct epithelium in infancy. tunica fibromuscularis of extrahepatic bile ducts consists of connective tissue. increase of collagen fibers and fibers stained by resorcin fuchsin was observed during development. neither in hepatic bile duc ... | 1988 | 3223595 |
| biomechanical properties of the canine knee articular cartilage as related to matrix proteoglycans and collagen. | | 1988 | 3224734 |
| [what is your diagnosis? orf of pyogenic granuloma type]. | | 1988 | 3232912 |
| do data from a previous exercise test influence the prognostic information given by a second routine exercise ecg test? a 13 1/2-year follow-up study in apparently healthy middle aged men. | a positive exercise ecg test in a middle-aged man without confounding disease is a strong indicator of occult coronary heart disease. in the years following a positive test mortality, but chd events vary considerably compared with subjects having normal exercise tests. if a second exercise test is positive in the same subjects years later, this signifies a disease which is prone to progress in a very severe way in the ensuing years. a first normal test followed by a second pathologic test has an ... | 1988 | 3246244 |
| do data from a previous exercise test influence the prognostic information given by a second routine exercise ecg test? a 13 1/2-year follow-up study in apparently healthy middle aged men. | a positive exercise ecg test in a middle-aged man without confounding disease is a strong indicator of occult coronary heart disease. in the years following a positive test mortality, but chd events vary considerably compared with subjects having normal exercise tests. if a second exercise test is positive in the same subjects years later, this signifies a disease which is prone to progress in a very severe way in the ensuing years. a first normal test followed by a second pathologic test has an ... | 1988 | 3246244 |
| use of haemophilus polysaccharide vaccine in a rural clinic setting. | | 1988 | 3258942 |
| are idiotypic peptides from variable domains critical for t-dependent production of rheumatoid factors? | studies from this laboratory have indicated that isologous myeloma protein 315 (m315, isotype iga, lambda 2) elicits t helper cells which recognize processed forms (peptides) of its v-domains and that recognition is controlled by h-2 linked immune-response (ir) genes (j exp med 1982; 155:1587-96; eur j immunol 1986; 16:889-93). we now report that adjuvant-free soluble m315, particularly the mildly reduced and alkylated form, stimulates a t-dependent antibody response mainly specific for m315's p ... | 1988 | 3266365 |
| collagen fiber alignment in the human preauricular dermis before and after rhytidectomy--a scanning electron microscopy study. | the microarchitecture of collagen fibers in preauricular dermis specimens that were obtained during two-phase rhytidectomy were visualized and recorded using the scanning electron microscope. examination of photomicrographs revealed that both spatial arrangement and degree of convolution of the collagen fibers were completely different in the specimens of the two phases. the results are interpreted in terms of the viscoelastic properties of the skin. | 1986 | 3273035 |
| differential regulation of amyloid-beta-protein mrna expression within hippocampal neuronal subpopulations in alzheimer disease. | we have mapped the neuroanatomical distribution of amyloid-beta-protein mrna within neuronal subpopulations of the hippocampal formation in the cynomolgus monkey (macaca fascicularis), normal aged human, and patients with alzheimer disease. amyloid-beta-protein mrna appears to be expressed in all hippocampal neurons, but at different levels of abundance. in the central nervous system of monkey and normal aged human, image analysis shows that neurons of the dentate gyrus and cornu ammonis fields ... | 1988 | 3277189 |
| echocardiographic assessment of cardiac function during respiratory syncytial virus infection. | respiratory syncytial virus infection has been associated with increased morbidity and mortality in infants with underlying cardiac and pulmonary disease. to understand better the cardiopulmonary interaction in patients with acute respiratory syncytial virus bronchiolitis, we performed m-mode echocardiograms and pulsed doppler assessment of pulmonary arterial flow in 19 patients with structurally normal hearts during acute illness. studies were repeated in 11 of these patients following complete ... | 1988 | 3285314 |
| echocardiographic assessment of cardiac function during respiratory syncytial virus infection. | respiratory syncytial virus infection has been associated with increased morbidity and mortality in infants with underlying cardiac and pulmonary disease. to understand better the cardiopulmonary interaction in patients with acute respiratory syncytial virus bronchiolitis, we performed m-mode echocardiograms and pulsed doppler assessment of pulmonary arterial flow in 19 patients with structurally normal hearts during acute illness. studies were repeated in 11 of these patients following complete ... | 1988 | 3285314 |
| alpha-sarcin cleaves ribosomal rna at the alpha-sarcin site in the absence of ribosomal proteins. | alpha-sarcin is capable of specifically cleaving the single phosphodiester bond in the "alpha-sarcin site" of both escherichia coli and saccharomyces cerevisiae large rrnas in the absence of ribosomal proteins. with both sources of rrna, the rate of cleavage was comparable with and without ribosomal proteins but more complete cleavage was observed in the absence of ribosomal proteins. these observations contrast with earlier findings and indicate that ribosomal proteins are not essential to the ... | 1988 | 3293566 |
| [experimental studies on the prostate epithelium. i. establishment of primary cell culture of rat dorsolateral prostate epithelium]. | | 1988 | 3294482 |
| acetoacetyl-acyl carrier protein synthase, a potential regulator of fatty acid biosynthesis in bacteria. | the first condensation reaction in the fatty acid biosynthetic pathway in escherichia coli was rate-limiting as judged by analysis of the relative pool sizes of acyl carrier protein (acp) thioester intermediates in vivo. comparable concentrations of acetyl-acp, malonyl-acp, and nonesterified acp were present during logarithmic growth, whereas long-chain acyl-acp comprised a minor fraction of the total acp pool. the antibiotic cerulenin was used to irreversibly inhibit both beta-ketoacyl-acp synt ... | 1987 | 3294837 |
| a simple method to eliminate the antigenicity of surface class i mhc molecules from the membrane of viable cells by acid treatment at ph 3. | we describe here a simple, reproducible method which specifically eliminates the antigenicity of surface class i major histocompatibility complex (mhc) molecules by acid treatment at ph 3 from the membrane of viable cells. when fresh mononuclear cells (mnc) or established cultured cell lines were treated at 4 degrees c for 2 min with citric acid buffer at ph 3 containing 1% bovine serum albumin, the antigenicity of class i mhc molecules, but not those of class ii mhc and the other non-mhc antige ... | 1987 | 3298442 |
| [antidiuretic mechanism in rhodnius prolixus (stal, 1859) (hemiptera, reduviidae)]. | an antidiuretic mechanism is proposed for rhodnius prolixus, whose activity is manifested when the diuretic phase has terminated and is maintained until the insect has fed again. presumably this mechanism acts at the level of the proximal rectal sphincter and is inhibited by ingestion, mechanical distension of the gut and central disinhibition by decapitation. it is suggested that the antidiuretic activity is maintained by the nervous system and is modified when there is distention of the gut or ... | 1986 | 3302595 |
| strongyloidiasis is endemic in another australian population group: indochinese immigrants. | the prevalence of antibodies against strongyloides in the serum of newly-arrived indochinese immigrants was determined. sera that had been stored in the state health laboratories, perth, were stratified according to age and sex, then samples were selected at random. two hundred and ninety sera that had been obtained from persons between the ages of one and 69 years were examined by an indirect fluorescent antibody test. five per cent of children who were aged between one and two years gave posit ... | 1987 | 3302635 |
| [functional morphology of the maxillo-mandibular system in the mini-lewe minipig. 4. functional analysis of the masticatory muscles at various ages]. | the percentage of dry weight distribution of the jaw muscles is described, and muscle growth is calculated by means of second degree homogeneous linear differential equations with constant coefficients. the components of the chewing muscles are calculated from the parallelogram of forces on the basis of their dry weights. changes in the shape of the skull during postnatal development leads to an increase in the adductive components and a reduction of the protractive components. changes in the re ... | 1987 | 3314594 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| improved insulin action in muscle, liver, and adipose tissue in physically trained human subjects. | the present studies were initiated to assess the effect of insulin on muscle, liver, and adipose tissue in eight control and eight physically trained individuals matched for age and body mass index. results indicated that percent body fat was 53% lower and maximal oxygen consumption 50% higher in physically trained subjects. although the plasma glucose response to a standard oral glucose challenge was similar in the two groups, the insulin response was significantly lower in the trained individu ... | 1987 | 3318492 |
| anti-candida drugs--the biochemical basis for their activity. | the past years have seen a continuous effort toward the synthesis of new antifungal agents. most of them belong to the n-substituted imidazoles and triazoles. another interesting series of antifungals are the allylamines. biochemically, both the azole derivatives and the allylamines belong to the class of ergosterol biosynthesis inhibitors and thus differ from the polyene macrolide antibiotics. indeed, it is now believed that the antifungal action of the polyenes, nystatin and amphotericin b, is ... | 1987 | 3319421 |
| biological markers during early pregnancy: trophoblastic signals of the peri-implantation period. | the peri-implantation period extends from the time the blastocyst is free in the uterus, through the processes of recognition and attachment, to the beginning of trophoblast differentiation and the interactions between the embryo and the uterine endometrium which initiate establishment of the hemochorial placenta. it is during the peri-implantation period that the embryo and hormonally regulated endometrial cells appear to be most sensitive to factors which introduce risk into the intrauterine e ... | 1987 | 3319548 |
| attempts to establish phage typing as an epidemiological marker for salmonella enteritidis. | the notable increase in the number of salmonella enteritidis strains seen in spain in recent years (from 27.10% in 1980 to 66.36% in 1985) makes it necessary to find an additional epidemiological marker for this serotype. phage typing was considered because of its discriminatory capacity toward other salmonella serotypes. wild and lysogenic bacteriophages were sought for a set of autochthonous bacteriophages. our set consisted of 6 bacteriophages, 5 wild and 1 lysogenic. when tested on 1,500 sel ... | 1987 | 3326630 |
| corticosteroids stimulate an increase in phospholipase a2 inhibitor in human serum. | a corticosteroid induced increase in a circulating inhibitor of serum phospholipase a2 activity is described. inhibitor activity was found to be normally present in serum in agreement with the findings of other workers, and this activity was significantly increased by either acute or chronic administration of corticosteroids. the possible relation of this inhibitor to the known inhibitory effects of lipocortin and sphingomyelin on phospholipase a2 activity is briefly discussed. | 1988 | 3347066 |