| postsurgical zosteriform herpes simplex 2 in noncontiguous dermatomes. | | 1990 | 2174931 |
| sensitive enzyme-linked immunosorbent assay for antibody to varicella-zoster virus using purified vzv glycoprotein antigen. | the development of an elisa of increased sensitivity has permitted a more critical evaluation of human humoral immune responses to the live attenuated varicella (oka/merck) vaccine. for use as a solid-phase antigen, the glycoprotein (gp) antigens are prepared by lectin-affinity chromatography from lysates of vzv-infected mrc-5 cells. the lot-to-lot variation in vzv gp content is controlled by standardization of antigen against a panel of human serum providing antigen-coated plates of consistent ... | 1990 | 2177782 |
| immunoenzymatic staining of viral and chlamydial antigens in cell culture. | methods are described for the conjugation of antibodies with biotin and for the use of these reagents in an immunoperoxidase staining procedure for infected cell cultures. this technique provides a simple, rapid, and specific approach to the identification and characterization of a number of viral and chlamydial isolates in the diagnostic laboratory. | 1985 | 2411466 |
| t lymphocyte responses to coxsackie b4 and mumps virus. ii. immunoregulation by hla-dr3 and -dr4 associated restriction elements. | a major part of the t lymphocyte response to mumps and coxsackie b4 virus appears to be restricted by hla-dr associated restriction elements. this was further corroborated in inhibition experiments using monoclonal antibodies reactive with different hla class ii molecules. only antibodies reactive with dr molecules significantly inhibited the response. the frequencies of dr restricted antigen-reactive t lymphocytes (artl) to mumps and coxsackie b4 virus were then investigated, using a limiting d ... | 1985 | 2413585 |
| immunologic and clinical responses to varicella-zoster virus-specific transfer factor following marrow transplantation. | | 1985 | 2415645 |
| induction of neutralizing antibody against varicella-zoster virus (vzv) by vzv gp3 and cross-reactivity between vzv gp3 and herpes simplex viruses gb. | glycoprotein gp3 (64k) is one of the major proteins specified by varicella-zoster virus (vzv). this glycoprotein was purified on an immunoadsorbent consisting of monoclonal antibody (clone 8) against gp3 linked to protein a-sepharose. rabbits were then immunized with the purified antigen to obtain monospecific antisera against gp3. the monospecific antisera and monoclonal antibody immunoprecipitated polypeptides with the same molecular weights of approximately 64,000 (64k), 106k, and 116k from a ... | 1986 | 2418583 |
| neutralization epitope of varicella zoster virus on native viral glycoprotein gp118 (vzv glycoprotein gpiii). | varicella-zoster virus (vzv) specifies the formation of several glycoproteins, including a 118,000-da mature structural product (gp118). the biologic and biochemical properties of gp118 were studied after production of murine monoclonal antibodies to both a lowpassage laboratory strain (vzv-32) and an attenuated vaccine strain (vzv-oka). structural analyses performed with the three glycosidases endo-beta-n-acetylglucosaminidase h (endoglycosidase h), endo-beta-n-acetylglucosaminidase f (endoglyc ... | 1986 | 2418586 |
| trans-activation of the human immunodeficiency virus long terminal repeat sequence by dna viruses. | to investigate whether dna viruses can augment gene expression of the human immunodeficiency virus (hiv), cotransfection experiments were carried out in which a recombinant plasmid containing the hiv long terminal repeat (ltr) linked to the chloramphenicol acetyltransferase (cat) gene was transfected into cultured cells along with plasmids containing dna from various distinct classes of dna viruses. molecular clones containing jc virus, bk virus, lymphotropic papovavirus, bovine papilloma virus, ... | 1986 | 2432602 |
| enzyme immunofiltration staining assay for immediate diagnosis of herpes simplex virus and varicella-zoster virus directly from clinical specimens. | a simple, sensitive, 30-min assay for the detection of herpes simplex virus (hsv) and varicella-zoster virus (vzv) antigens in clinical specimens is described. the assay utilizes a filter manifold, biotinylated monoclonal antibodies, streptavidin-horseradish peroxidase conjugate, and the substrate aminoethylcarbazole. the method stains infected cells and cell debris a bright red and is easily interpreted with a dissecting microscope. reconstruction experiments indicated that as few as two virus- ... | 1987 | 2434523 |
| polyadenylated, cytoplasmic transcripts of varicella-zoster virus. | cytoplasmic rna was isolated from varicella-zoster virus (vzv)-infected cells. by oligo(dt)-cellulose chromatography, the rna was separated into polyadenylated, poly (a)+, and nonpolyadenylated, poly (a)-, fractions. rna blot hybridization was employed to detect and map vzv transcripts. as vzv infection cannot be coordinated, cytoplasmic rna was isolated from vzv-infected cells when the cells showed extensive cytopathology. therefore, while the vzv transcripts represented heterogeneous temporal ... | 1986 | 2440829 |
| antiviral effects of recombinant human tumor necrosis factor. | the antiviral action of recombinant human tumor necrosis factor (tnf) was studied using assay systems to determine inhibition of viral cytopathic effect (cpe), as well as suppression of virus growth measured by plaque assays. tnf was cloned and prepared by asahi chemical industry, japan. antiviral activity against human herpes simplex virus (hsv) types 1 and 2, cytomegalovirus (cmv), varicella-zoster virus (vz), vesicular stomatitis virus (vsv) and encephalomyocarditis virus (emc), was demonstra ... | 1987 | 2448553 |
| recognition of similar epitopes on varicella-zoster virus gpi and gpiv by monoclonal antibodies. | two monoclonal antibodies, mab43.2 and mab79.0, prepared against varicella-zoster virus (vzv) proteins were selected to analyze vzv gpiv and gpi, respectively. mab43.2 reacted only with cytoplasmic antigens, whereas mab79.0 recognized both cytoplasmic and membrane antigens in vzv-infected cells. immunoprecipitation of in vitro translation products with mab43.2 revealed only proteins encoded by the gpiv gene, whereas mab79.0 precipitated proteins encoded by the gpiv and gpi genes. pulse-chase ana ... | 1988 | 2455814 |
| varicella in pediatric orthotopic liver transplant recipients. | from may 1981 to may 1984, 90 pediatric patients underwent liver transplantation and 65 patients survived as of may 1986. two of the nonsurvivors died with complications related to clinical varicella. of these 67 patients (65 survivors and two nonsurvivors who died of varicella-related causes), 51 patients were determined to be varicella susceptible. clinical disease developed in no patients with serologic evidence or clinical history of varicella prior to transplantation. eighteen susceptible p ... | 1989 | 2536479 |
| western blot analysis poorly detects antibody to gpiii of varicella-zoster virus. | | 1989 | 2536785 |
| igm and igg responses to varicella-zoster virus p32/p36 complex after chickenpox and zoster, congenital and subclinical infections, and vaccination. | varicella-zoster virus (vzv) directs the synthesis of a nonglycosylated polypeptide complex with two prominent species with relative molecular masses of 32,000 and 36,000; the p32/p36 complex is present in both the viral nucleocapsid and the nuclear matrix of the infected cell. we have now further characterized the humoral immune response of vzv-infected humans to this complex and established that it is a major viral antigen. antibodies to vzv p32/p36 were detected in sera from patients with bot ... | 1989 | 2536788 |
| varicella zoster-associated neurologic disease without skin lesions. | we have investigated a group of cases with inflammatory neurologic disease and serologic evidence of varicella zoster virus activity without cutaneous vesicles. in agreement with experience, a case of cranial polyneuropathy with facial palsy and cases of aseptic meningitis were found. however, cases of acute polyneuropathy, myelitis, and encephalitis were also identified. thus, it seems that the spectrum of neurologic disease associated with varicella zoster virus in the absence of skin lesions ... | 1989 | 2537615 |
| titration of igg antibodies against varicella zoster virus before bone marrow transplantation is not predictive of future zoster. | serum antibodies to varicella zoster virus (vzv) were measured in 77 patients about to undergo allogeneic bone marrow transplantation, and in 65 of their donors. ten patients developed zoster within the first 6 months following transplant. there was no significant difference in the mean pretransplant antibody titre between those patients who did or did not subsequently develop zoster. likewise, the level of antibody to vzv amongst donors had no effect on the subsequent development of zoster. we ... | 1989 | 2537881 |
| investigation of varicella-zoster virus infection of lymphocytes by in situ hybridization. | peripheral blood mononuclear cells harboring viral gene sequences were detected during primary varicella-zoster virus (vzv) infection of the human host and the strain 2 guinea pig by in situ hybridization with a 3h-labeled vzv dna probe. activated t lymphocytes were permissive for vzv infection at low frequency in vitro. | 1989 | 2539528 |
| screening for varicella-zoster immunity and the use of varicella zoster immune globulin in pregnancy. | | 1989 | 2540653 |
| varicella-zoster virus infection of adult rat sensory neurons in vitro. | we report here an in vitro model of neuronal infection by varicella-zoster virus (vzv). such a model has been achieved by using dissociated adult rat dorsal root ganglia cells infected by cocultivation with vzv-infected mrc5 cells or with cell-free virus. indirect vzv immunolabeling, in situ hybridization, and neuron-specific immunolabeling demonstrated that vzv infection occurred selectively in neurons. vzv-specific immunolabeling detected a few neurons 1 or 2 days postinfection but not later. ... | 1989 | 2542622 |
| [efficacy of live varicella vaccine strain (oka) in high-risk patients]. | | 1989 | 2542665 |
| syndrome of inappropriate secretion of antidiuretic hormone (siadh) associated with ramsay hunt syndrome: report of a case and review of the literature. | a case of syndrome of inappropriate secretion of antidiuretic hormone (siadh) associated with ramsay hunt syndrome is reported. a 59-year-old man was admitted to our department for treatment of left facial pain that had persisted for three days. a left renal tumor had been diagnosed and a radical nephrectomy had been performed two months earlier. on admission, vesicular lesions were found in the left external auditory canal and ear lobe. additionally found were left facial nerve palsy and hearin ... | 1989 | 2543853 |
| comparative restriction endonuclease analysis of varicella-zoster virus clinical isolates. | the dnas of 67 isolates of varicella-zoster virus (vzv) obtained from 31 individuals were compared by restriction endonuclease analysis using bamhi, ecori, psti and smai. all of the epidemiologically unrelated 26 isolates could be differentiated using smai and another one or two enzymes. however, the dna cleavage profiles of multiple vzv isolates from the same patient and the isolates from a group of patients who were infected with vzv from the same source were found to be identical to each othe ... | 1989 | 2543893 |
| epstein-barr virus: the hematologic and oncologic consequences of virus-host interaction. | varicella-zoster virus (vzv) and epstein-barr virus (ebv) are two of the human herpesviruses. the others include herpes simplex virus (hsv) type 1, hsv type 2, and cytomegalovirus (cmv). in a series of two articles, we review the clinical diseases caused by vzv and ebv infections; we pay particular attention to the manifestations of these two viral infections in immunosuppressed and immunocompromised patients. in addition to the clinical reviews, each of the two articles begins with a brief disc ... | 1989 | 2545365 |
| [human herpesvirus 6 (hhv-6): incidence in healthy blood donors and in patients with acute infections caused by other herpes viruses]. | antibodies to hhv-6 were detected by immunofluorescence in 8.04% of 460 healthy blood donors in west austria. testing sera from patients with acute or reactivated infections with other herpesviruses, such as cytomegalovirus (cmv), epstein-barr virus (ebv), herpes simplex virus (hsv) and varicella zoster virus (vzv) we observed a remarkably higher prevalence of antibodies to hhv-6 in patients with cmv infections (75%) and also in patients with ebv infections (50%). patients with hsv and vzv infec ... | 1989 | 2548350 |
| transcription mapping of glycoprotein i (gpi) and gpiv of varicella-zoster virus and immunological analysis of the gpi produced in cells infected with the recombinant vaccinia virus. | in order to determine the transcripts of gpi and gpiv of varicella-zoster virus (vzv), rna was isolated from human embryonic fibroblast cells infected with vzv and northern blot analysis was carried out using cloned dna probes of unique short region including gpi and gpiv genes. the analysis of rna revealed two discrete transcripts of 3.6 and 2.15 kilobases (kb) and three transcripts of 3.6, 2.9, and 1.6 kb which hybridized to dna probes covering the gpi and gpiv region, respectively. next, mrna ... | 1989 | 2549343 |
| mechanism of selective inhibition of varicella zoster virus replication by 1-beta-d-arabinofuranosyl-e-5-(2-bromovinyl)uracil. | to investigate the mechanism of action of 1-beta-d-arabinofuranosyl-(e)-5-(2-bromovinyl)uracil (bv-arau) on varicella zoster virus (vzv) replication, we examined the metabolism of the drug in vzv-infected cells using 14c-labeled bv-arau. [14c]bv-arau was taken up by the cells infected with thymidine kinase-positive (tk+)vzv, but not so much by tk- vzv-infected or mock infected cells. most of the radioactivity in tk+ vzv-infected cells that were incubated with [14c]bv-arau was recovered from thei ... | 1989 | 2549386 |
| humoral immunity and clinical reinfections following varicella vaccine in healthy children. | the duration of immunity following varicella-zoster vaccination in healthy children remains a critical issue. in a 3-year study of 140 oka/merck vaccine recipients, duration of immunity was assessed by two measures. the first was persistence of varicella-zoster antibody measured by modified fluorescent antibody to membrane antigen test. thirty-six toddler vaccinees 12 to 24 months of age had sera obtained at 6 weeks, 1 year, and 2 years. geometric mean titer +/- sd at 6 weeks was 57.7 +/- 2.9; a ... | 1989 | 2549498 |
| pathogenesis of congenital infection with three diverse viruses: varicella-zoster virus, human parvovirus, and human immunodeficiency virus. | | 1989 | 2549641 |
| immunological diagnosis in viral infections of the central nervous system: course of antibody titres against homo- and heterologous viruses. | in clinical cases suspected for viral encephalitis or meningoencephalitis, the estimation of virus-specific antibodies especially in liquor requires high sensitivity as well as specificity. with enzyme immunoassays the sensitivity in detecting antibodies has increased compared to e.g., complement fixation tests. this report concerns the determination of virus-specific antibodies with a commercial enzyme-linked immunosorbent assay (elisa) in paired liquor/serum samples of four patients with encep ... | 1989 | 2550755 |
| evidence for attachment of fatty acid to varicella-zoster virus glycoproteins and effect of cerulenin on the maturation of varicella-zoster virus glycoproteins. | varicella-zoster virus (vzv) contains four major glycoproteins designated gpi, gpii, gpiii, and gpiv. in the present study the presence and role of fatty acids in these glycoproteins were examined. the incorporation of fatty acid into the glycoproteins that span the membranes of vzv was studied. for this, virus-infected human embryo fibroblast cells were labeled with [3h]-palmitic acid, an extract of the cells was treated with monoclonal antibodies to vzv glycoproteins, and the precipitate was a ... | 1989 | 2551842 |
| human herpesviruses: a consideration of the latent state. | | 1989 | 2552271 |
| varicella-zoster virus infections: chronic disease in the immunocompromised host: evidence for persistent excretion of virus. | | 1989 | 2552389 |
| difficulty in determining varicella-zoster immune status in pregnant women. | | 1989 | 2552391 |
| the genomes of the human herpesviruses: contents, relationships, and evolution. | | 1989 | 2552898 |
| mumps, measles, and rubella vaccination and encephalitis. | | 1989 | 2553173 |
| [problems in the management of drug-resistant herpesvirus infections]. | | 1989 | 2553388 |
| [herpes virus infections in immunosuppressed patients. herpes simplex and varicella zoster virus: prevention and therapy]. | herpes simplex virus (hsv) and varicella-zoster virus (vzv) giving rise to primary or recurrent infections still threaten immunocompromised patients. however, such prophylactic measures as passive and active immunisation against vzv infection, and antiviral chemoprophylaxis and chemotherapy of hsv- and vzv-infections have improved the prognosis of these patients considerably. | 1989 | 2553562 |
| molecular cloning of human uracil-dna glycosylase, a highly conserved dna repair enzyme. | uracil-dna glycosylase is the dna repair enzyme responsible for the removal of uracil from dna, and it is present in all organisms investigated. here we report on the cloning and sequencing of a cdna encoding the human uracil-dna glycosylase. the sequences of uracil-dna glycosylases from yeast, escherichia coli, herpes simplex virus type 1 and 2, and homologous genes from varicella-zoster and epstein-barr viruses are known. it is shown in this report that the predicted amino acid sequence of the ... | 1989 | 2555154 |
| study of virus isolation from pharyngeal swabs in children with varicella. | we performed virus isolations from the pharyngeal swabs in 117 children with varicella who were aged from 22 days to 15 years and 70 healthy children who were aged from 3 months to 15 years, by using human embryonic lung cell cultures. viral isolates were confirmed by an indirect immunofluorescence method or by neutralization with well-characterized antibodies. five varicella-zoster virus isolates (4.3%), 23 cytomegalovirus isolates (19.7%), five herpes simplex virus isolates (4.3%), and one res ... | 1989 | 2556024 |
| [chickenpox? but... she could have a baby any moment now!]. | | 1989 | 2556646 |
| mapping of two varicella-zoster virus-encoded genes that activate the expression of viral early and late genes. | a transient assay system was used to identify varicella-zoster virus (vzv)-encoded genes whose products are able to activate the expression of an early gene promoter, the thymidine kinase (tk) promoter, and a late gene promoter, and the glycoprotein i (gpi) promoter. vero cells were cotransfected with individual cloned dna fragments spanning the entire vzv genome and with the recombinant construct p1tkcat which contained the chloramphenicol acetyl transferase (cat) gene under the control of puta ... | 1989 | 2556848 |
| association of steroid therapy with vaccine-associated rashes in children with acute lymphocytic leukaemia who received oka/merck varicella vaccine. niaid varicella vaccine collaborative study group. | oka strain varicella vaccine generally has been well tolerated by children with acute lymphocytic leukaemia (all) in remission and has induced protection against disease caused by wild-type varicella virus. at the end of 1985, four extensive vaccine-associated rashes were reported among children on maintenance chemotherapy. steroid therapy in the week before vaccination or in the week following vaccination was significantly associated with rash in a retrospective analysis (odds ratio = 3.84, p = ... | 1989 | 2558454 |
| distribution of varicella-zoster virus strains carrying a psti-site-less mutation in japan and dna change responsible for the mutation. | we have analyzed 53 varicella-zoster virus (vzv) strains isolated in japan for the presence of a psti cleavage site in a middle portion of the long unique region of vzv genome. about 25% of the strains examined did not have this psti cleavage site, although the majority of strains retained this site. for several vzv strains lacking this psti cleavage site, we sequenced a recombinant dna-derived short segment containing the mutation. in all strains analyzed, we identified a base replacement from ... | 1989 | 2560086 |
| clinical experience of oka-strain live varicella vaccine. | | 1989 | 2561119 |
| varicella zoster virus and recurrent aphthous ulceration. | | 1989 | 2566763 |
| outcome in newborn babies given anti-varicella-zoster immunoglobulin after perinatal maternal infection with varicella-zoster virus. | 281 newborn babies whose mothers had chickenpox and 25 whose mothers had herpes zoster during the perinatal period were investigated. igg antibody was present at birth in all babies born more than 7 days after the onset of maternal chickenpox. when the mother's rash appeared 7-3 days before delivery progressively fewer babies were born with antibody, and no infant born less than 3 days after the onset had antibody at birth. infants were given 100 or 250 mg of anti-varicella-zoster immunoglobulin ... | 1989 | 2569560 |
| humoral and cellular immunity to varicella-zoster virus glycoprotein gpi and to a non-glycosylated protein, p170, in the strain 2 guinea-pig. | strain 2 guinea-pigs were inoculated with infectious varicella-zoster virus (vzv) or with immunoaffinity-purified proteins of vzv. monoclonal antibodies to the vzv gpi (90,000/58,000 complex) and to a non-glycosylated protein, p170, were used to prepare the polypeptide antigens. humoral and cell-mediated immune responses to the infectious virus were compared with those elicited by the gpi and p170 proteins. both vzv igg antibody production and t lymphocyte proliferation to vzv were detected afte ... | 1987 | 2821181 |
| [herpes zoster]. | | 1987 | 2821453 |
| varicella in children with cancer: impact of antiviral therapy and prophylaxis. | to estimate the impact of antiviral therapy and prophylaxis on the natural course of the infection, 288 cases of varicella in children with cancer were reviewed. among 127 patients with untreated infections, the overall mortality rate was 7%. varicella-zoster virus pneumonitis developed in 28% of the untreated patients and was associated with a 25% mortality rate. pneumonitis was much more likely to develop in patients with acute leukemia than in those with other malignancies (32% v 19%). simila ... | 1987 | 2821476 |
| [(e)-5-(2-bromovinyl)-2'-desoxyuridine--a new nucleoside analog with selective inhibitory action against herpesviruses. studies in cell culture and animal experiments]. | (e)-5-(2-bromovinyl)-2'-deoxyuridine (1; brvudr) inhibits the replication of herpes simplex virus type 1 (hsv-1) and of varicella-zoster virus (vzv) in vitro at concentrations of 0.01 to 0.23 mumol/l, whereas herpes simplex virus type 2 (hsv-2) is influenced only at 5.5 to 27 mumol/l. in comparison to some classical and newly developed antiherpetics, i. e. 5-iodo-2'-desoxyuridine (2; idoxuridine, idu), 9-beta-d-arabinofuranosyladenine (4; vidarabine ara-a), 9-(2-hydroxyethoxymethyl) guanine (5; ... | 1987 | 2823299 |
| detection of varicella-zoster virus nucleic acid in neurons of normal human thoracic ganglia. | tissue sections from four normal human thoracic ganglia were hybridized in situ with a varicella-zoster virus-rna probe. varicella-zoster virus was detected in two of four ganglia, localized exclusively in neurons. the detection of latent varicella-zoster virus genetic material in thoracic ganglia provides further evidence of varicella-zoster virus latency at multiple levels of the human neuraxis and supports the notion that the neuron is the primary site of herpesvirus latency. | 1987 | 2823688 |
| non-herpes simplex encephalitis is early exclusion of herpes simplex etiology possible? | since effective antiviral treatment is available for herpes simplex encephalitis (hse), early diagnosis or exclusion of herpes simplex etiology is essential for prognosis. in a retrospective study of 25 cases of acute viral encephalitis not caused by herpes simplex virus (non-hse), we investigated whether hse can be excluded in the early phase before serological evidence is present. using clinical means, history, investigations of csf (protein, cells), eeg, and cct, hse could not be excluded wit ... | 1987 | 2824204 |
| detection of antibody to viral proteins following primary infection with herpes simplex virus. | sera from patients with primary genital infection with herpes simplex virus (hsv) were tested by immunoblotting (ib) and radioimmunoprecipitation (rip) analysis to determine the protein targets of antibody elicited by infection. the two tests detected antibody to different antigens: ib primarily detected reactivity with p40, a phosphorylated capsid protein, and rip detected antibody to glycoprotein b, a viral envelope component. furthermore, rip detected antibody at an earlier stage of infection ... | 1987 | 2824685 |
| ribonucleotide reductase induced by varicella zoster virus. characterization, and potentiation of acyclovir by its inhibition. | an enzyme that catalyzes the conversion of cdp to 2'-dcdp in the presence of dithiothreitol (dtt) was detected in ammonium sulfate fractionated-extracts of varicella zoster virus (vzv)-infected cells. this ribonucleotide reductase was antigenically distinguishable from the isofunctional eucaryotic enzyme as well as the ribonucleotide reductases induced by herpes simplex virus types 1 and 2 (hsv-1 and hsv-2). the vzv-induced enzyme was purified to the extent that most of the contaminating enzymes ... | 1987 | 2825724 |
| processing and presentation of cell-associated varicella-zoster virus antigens by human monocytes. | to determine whether viral antigens associated with infected cells were processed for presentation to t cells, we cultured human blood mononuclear cells (mnc) from varicella-zoster virus (vzv) immune donors with vzv-infected fibroblasts of known hla type which had been fixed in 0.05% glutaraldehyde. after 7-8 days of culture thymidine uptake by t4+ cells exceeded that of t8+ cells. stimulated cells were depleted of adherent cells and restimulated with vzv-infected fibroblasts from donors matched ... | 1987 | 2826056 |
| [acute lymphocytic meningitis with intrathecal production of igg-specific varicella zoster virus antibodies]. | | 1987 | 2826940 |
| nosocomial varicella: worth preventing, but how? | | 1988 | 2827533 |
| a simplified plaque assay for varicella vaccine. | a simple and accurate plaque assay is described for potency testing of attenuated varicella vaccine. assays were performed on the african green monkey kidney continuous cell line cv-1, in multidish-plates, under a semi-solid carboxymethylcellulose overlay. the test is economical and yields accurate individual titre estimates, the reliability of which may be assessed by parallel titration of reference preparations. | 1987 | 2828397 |
| variation of r1 repeated sequence present in open reading frame 11 of varicella-zoster virus strains. | we molecularly cloned the tandem direct reiteration (r1) present in open reading frame (orf) 11 from three independent strains of varicella-zoster virus. comparison of the r1 sequences among varicella-zoster virus strains revealed that, although the portion of r1 near the 5' terminus of orf 11 was conserved among strains, the 3'-terminal portion varied remarkably. this variation was due to the different arrangement of two elements (a and b) and a segment produced by fusion of a and b and to a si ... | 1988 | 2828661 |
| chronic hiv encephalitis--i. cerebrospinal fluid diagnosis. | to establish a reliable procedure for the early detection of central nervous system involvement in hiv infection, paired cerebrospinal fluid and serum samples of 59 patients were analysed. fifteen were hiv antibody positive without clinical symptoms (stage i), 12 had lymphadenopathy syndrome or aids-related complex (stage ii), and 32 had aids (stage iii). intrathecal synthesis of hiv antibodies was determined by a modified elisa. antibodies in csf and serum were evaluated at identical immunoglob ... | 1988 | 2830432 |
| a gene for dihydrofolate reductase in a herpesvirus. | the enzyme dihydrofolate reductase (dhfr) is found ubiquitously in both prokaryotes and eukaryotes. it is essential for de novo synthesis of purines and of deoxythymidine monophosphate for dna synthesis. among viruses, however, only the t-even and t5 bacteriophage have been found to encode their own dhfr. in this study a gene for dhfr was found in a specific subgroup of the gamma or lymphotropic class of herpesviruses. dna sequences for dhfr were found in herpesvirus saimiri and herpesvirus atel ... | 1988 | 2830673 |
| human cytomegalovirus-specific cytotoxic t cells: their precursor frequency and stage specificity. | human virus-specific cytotoxic t (tc) cells may be important in maintaining the virus/host equilibrium during persistent herpes virus infections such as that with human cytomegalovirus (hcmv). we have previously shown that hcmv-specific tc cells are present in peripheral blood in normal asymptomatic seropositive individuals (l. k. borysiewicz et al., eur. j. immunol. 1983. 13: 804). in this study we have used limiting dilution analysis to estimate the precursor frequency of these tc cells and to ... | 1988 | 2832181 |
| severe and recurrent varicella-zoster virus infection in a patient with the acquired immune deficiency syndrome. | we report a case of recurrent varicella-zoster virus infection in a patient with severe acquired immune deficiency syndrome in whom the infection has become clinically unresponsive to treatment with acyclovir. | 1988 | 2832482 |
| [an electron microscopic observation on cells infected with varicella-zoster virus]. | | 1987 | 2833839 |
| enhancement of a secondary antibody response to vesicular stomatitis virus "g" protein by ifn-gamma treatment at primary immunization. | ifn-gamma is a secreted polypeptide product of stimulated t lymphocytes with immunomodulatory properties as well as antiviral activity. we have investigated the effects of ifn-gamma treatment on a neutralizing antibody response to vesicular stomatitis virus (vsv) when administered in conjunction with immunization using purified envelope glycoprotein "g" of vsv. administration of rifn-gamma to mice or cattle at the time of primary immunization with vsv g glycoprotein enhanced the magnitude of a s ... | 1988 | 2834443 |
| immunology of the varicella-zoster virus glycoproteins. | | 1988 | 2834465 |
| western blot analysis of antibody to varicella-zoster virus. | we used western blot (wb) to compare the igg response to varicella-zoster virus (vzv) after chickenpox (cp), zoster, and administration of a live attenuated varicella vaccine (lavv). after cp, 13 of 14 normal children had antibody to glycoprotein (gp) i (99 and 92 kilodaltons [kda]), 11 had antibody to gpii (133 kda), and 10 had antibody to gpiii (119 kda). bands at 150 and 35 kda were also seen in 13 and 11 sera, respectively. bands to gpi, gpii, and p35 were more intense after zoster than cp. ... | 1988 | 2834466 |
| [infection defense antigens against pathogenic viruses to human. infection defense antigens and genetic code--trend and progress of study on biomolecular analysis. f. varicella-zoster virus]. | | 1987 | 2834574 |
| a proposed mild type of acute retinal necrosis syndrome. | six patients had peripheral to midperipheral pale yellow retinal exudates typical of acute retinal necrosis syndrome but which extended gradually to the posterior pole and remained isolated without becoming confluent. these exudates resulted in localized retinochoroidal degeneration without retinal detachment, in contrast to acute retinal necrosis syndrome. antibody titers in aqueous humor were increased to varicella-zoster virus in some of the patients examined. the findings led us to hypothesi ... | 1988 | 2837090 |
| strain variation of r5 direct repeats in the right-hand portion of the long unique segment of varicella-zoster virus dna. | we located a region of interstrain size variability in a short segment in an area at the right-hand end of the long unique sequence of the varicella-zoster viral genome. varicella-zoster virus strains isolated in a district of japan were classified into three groups on the basis of the size of this segment. sequence comparison of the variable segment among strains from different groups revealed that the tandem direct repeat, r5, in the segment was variable among strains. r5, which was first disc ... | 1988 | 2839710 |
| varicella infection with profound neutropenia, multisystem involvement and no sequelae. | | 1988 | 2839816 |
| methyl gallate, methyl-3,4,5-trihydoxybenzoate, is a potent and highly specific inhibitor of herpes simplex virus in vitro. ii. antiviral activity of methyl gallate and its derivatives. | methyl gallate (mg), methyl-3,4,5-trihydroxybenzoate, was highly active against herpes viruses as determined by plaque reduction assay. herpes simplex virus type 2, ms strain, was sensitive to mg at a mean 50% inhibitory concentration (ic50) of 0.224 micrograms/ml in monkey kidney cells. mg was specific for herpes viruses with the relative sensitivity hsv-2 greater than hsv-1 greater than cmv. two rna viruses tested were significantly less sensitive to mg. the structural components of mg which m ... | 1988 | 2840133 |
| delayed type hypersensitivity (dth) skin reaction to varicella-zoster virus antigen in patients with systemic lupus erythematosus (sle). | | 1988 | 2842248 |
| mutational dissection of the hsv-1 immediate-early protein vmw175 involved in transcriptional transactivation and repression. | vmw175 is one of five immediate-early (ie) proteins encoded by herpes simplex virus type-1 (hsv-1). it is required for the transcription of later classes of genes and for the accompanying repression of ie expression. vmw175 has been shown to be a transactivator of transcription and also to autoregulate its own synthesis. we have made a large number of small, in-frame, insertion and deletion mutants of a plasmid-borne copy of the gene encoding vmw175. study of the activity of the resultant mutant ... | 1988 | 2842944 |
| regulation of varicella zoster virus gene 27 translation in vitro by upstream sequences. | northern blot analysis revealed the presence of varicella-zoster virus (vzv) gene 27 transcripts in infected cells. the sal i-g dna fragment, located in the unique long segment of the vzv genome and containing overlapping genes 26 and 27, was analyzed in an in vitro transcription-translation system. translation of rna transcribed from these open reading frames showed prominent expression of gene 27. four different subclones were constructed to contain gene 27 with and without 100 base pairs (bp) ... | 1988 | 2842970 |
| infection of undifferentiated and differentiated promyelocytic cells (hl-60) with varicella-zoster virus. | hl-60 cells can be induced to differentiate into macrophage-like cells by treatment with 12-o-tetra-decanoylphorbol-13-acetate (tpa). the relationship between virus replication and cell differentiation was investigated using hl-60 cells that had been induced to differentiate by tpa (dhl-60 cells) and undifferentiated cells (udhl-60 cells). on infection of these cells with cell-free varicella-zoster virus (vzv), virus antigens were detected in dhl-60 cells but not in udhl-60 cells, and the percen ... | 1988 | 2842978 |
| cerebral infarction in an adult with disseminated varicella. | a 26-year-old patient with disseminated varicella developed a cerebral infarction 11 days into the illness. cerebral infarction, typically delayed, is primarily reported in association with zoster with only one previous report in primary varicella. the mechanism is speculative and thrombosed vessels with and without vasculitis have been demonstrated. our patient showed mild evidence of systemic alteration in immune function, suggesting the possibility of vasculitis. | 1986 | 2843250 |
| viral infection in acute peripheral facial palsy. investigation in yamagata prefecture. | we studied viral involvement in bell's palsy and ramsay hunt syndrome. in the cf test, viral infection was confirmed in 2 of 84 patients. of the 12 patients with ramsay hunt syndrome, 5 showed a significantly high titre for varicella zoster and one for rubella. the elisa test, performed in 60 patients with bell's palsy, revealed significantly high titres for varicella zoster in 5 patients and for herpes simplex in 4 patients. all 5 patients with ramsay hunt syndrome showed significantly high tit ... | 1988 | 2844058 |
| varicella-zoster virus-specific hla-restricted cytotoxicity of normal immune adult lymphocytes after in vitro stimulation. | varicella-zoster virus (vzv)-specific cytotoxic t cell responses were studied in 35 presumably immune and two nonimmune adult donors and in two patients with herpes zoster. peripheral blood lymphocytes (pbls) were stimulated for five or 12 days with autologous, irradiated, vzv-infected pbls. cytotoxicity was measured in a chromium-release assay. target cells were usually cryopreserved, phytohemagglutinin-stimulated pbls, either infected with vzv or uninfected. in testing the presumably immune ad ... | 1988 | 2844916 |
| serology in facial paralysis caused by clinically presumed herpes zoster infection. | in some cases of peripheral facial palsy due to a clinically suspected varicella zoster virus (vzv) infection, the clinical diagnosis is not supported by serological tests. in a retrospective study, we examined the sera from 63 patients with clinical findings compatible with vzv infection: 57 had paired sera and 6 had single sera. in the paired-sera group, 18 cases were serologically negative initially by complement fixation (cf), while 9 of these cases were actually positive for vzv when cf was ... | 1988 | 2845904 |
| herpes simplex virus detection by macroscopic reading after overnight incubation and immunoperoxidase staining. | human diploid foreskin fibroblast cells grown in 24-well plates were inoculated with clinical specimens by centrifugation at 1,000 x g for 45 min. cultures were incubated at 37 degrees c overnight, fixed, and stained with peroxidase-labeled monoclonal antibodies against herpes simplex virus types 1 and 2. stained plaques of infected cells were large enough to be detected with the naked eye, and microscopic examination did not reveal any further positive specimens. the method was compared with st ... | 1988 | 2846634 |
| placental transfer of igg subclass-specific antibodies to varicella-zoster virus. | commercially available mouse monoclonal antibodies to human igg subclasses (igg 1 to igg 4) were used in an enzyme-linked immunosorbent assay to examine the placental transfer of igg subclass-specific antibodies to varicella-zoster virus (vzv). all 46 mothers had total igg antibody to vzv, and an equal level of antibody was found in the cord blood. all of the mothers had high antibody activities to vzv in igg 1 and igg 4 subclasses. the majority of the mothers were negative for igg 2 and igg 3 v ... | 1988 | 2846768 |
| [early diagnosis of varicella-zoster virus infection in acute facial paralysis--significance of vzv antigen skin test and csf examination]. | | 1988 | 2846806 |
| varicella exposure in a neonatal intensive care unit: case report and control measures. | forty-six infants in a neonatal intensive care unit and 138 health care workers were exposed to a pediatric medical resident during the prodromal period and the early days of unrecognized varicella. an attempt was made to prevent an outbreak of additional cases by the institution of emergency control measures. these measures included rapid identification of varicella antibody status in exposed neonates, varicella antibody testing of health care workers with unknown or uncertain history of varice ... | 1988 | 2847598 |
| igg subclass distribution of antiviral antibodies in common variable immunodeficiency: effect of substitution therapy. | immunoglobulin g subclass titers to three herpesviruses (herpes simplex; hsv; cytomegalovirus, cmv; varicella zoster virus, vzv) were examined in patients with common variable immunodeficiency (cvi) before and after immunoglobulin substitution. like healthy controls, cvi patients expressed igg1 and igg3 to hsv and cmv, but only igg1 to vzv. individual titers varied as in healthy individuals, but as a mean, specific igg titers were lowered in proportion to the decrease of total igg. hsv and cmv i ... | 1988 | 2847890 |
| immunity to whole varicella-zoster virus antigen and glycoproteins i and p170: relation to the immunizing regimen of live attenuated varicella vaccine. | humoral and cellular immune responses to whole varicella-zoster virus (vzv) antigen and the vzv proteins glycoprotein i (gpi) and nonglycosylated protein p170 were evaluated in healthy children and adults given lyophilized live-attenuated varicella vaccine. children received one dose of vaccine containing 950 pfu, whereas adults received two doses of 2500 pfu. after one year, the antibody titers of adult vaccinees to whole vzv and to gpi were significantly higher than those of children. antibody ... | 1988 | 2848901 |
| patterns of gene expression and sites of latency in human nerve ganglia are different for varicella-zoster and herpes simplex viruses. | the cellular localization and viral transcription patterns of acute and latent varicella-zoster virus (vzv) infections of human sensory nerve ganglia were studied by in situ hybridization and compared with those of latent herpes simplex virus (hsv) infection. trigeminal and dorsal root ganglia obtained at autopsy were hybridized with 35s-labeled single-stranded rna probes homologous to vzv or hsv fragments. we have reported that hsv persists in human sensory neurons and expresses only one family ... | 1988 | 2849116 |
| evaluation of varicella-zoster antiviral drugs by a nucleic acid hybridization assay. | a simple nucleic acid hybridization assay was developed for the evaluation of antiviral compounds with activity against varicella-zoster virus. antiviral-induced reduction in varicella-zoster virus dna, as measured by hybridization with 32p-labeled probes, correlated with drug-induced inhibition of viral cytopathic effect in cell culture. three compounds were shown to be more potent than acyclovir at inhibiting varicella-zoster virus in vitro. | 1988 | 2852488 |
| [interpretation of varicella serology in pregnancy]. | chickenpox during pregnancy may result in a severe, in some cases fatal, generalized infection of the newborn. pregnant women with unknown varicella-zoster-virus (vzv) immunity, who have had contact with an individual infected by chickenpox, should be tested immediately for specific antibodies to vzv. the interpretation of test results may be difficult under certain circumstances. this problem is discussed using individual case studies. | 1988 | 2853094 |
| antiherpesvirus activity and mechanism of action of indolo-(2,3-b)quinoxaline and analogs. | the antiherpesvirus activity of 14 derivatives of indoloquinoxaline was tested. the most active was 2,3-dimethyl(dimethylaminoethyl)5h-indolo-(2,3-b)quinoxaline, also called b-220. the antiherpesvirus mechanism of b-220 was sought. the compound inhibited replication of herpes simplex virus type 1, cytomegalovirus, and varicella-zoster virus in tissue culture at concentrations of 1 to 5 microm, depending on the cell type used for assay and the amount of virus. cellular toxicity was seen at a conc ... | 1988 | 2855298 |
| immunisation of nurses with a live varicella vaccine. | 34 nurses who had no previous history of chickenpox and were seronegative to varicella zoster virus (vzv) were immunised with a live attenuated varicella vaccine (oka-rit strain) and followed up for up to 36 months. no major vaccine reactions were observed. at 5 months and at 1 year, 94% of the nurses had seroconverted but at 3 years only 64% had detectable antibody. lymphocyte transformation to vzv antigen tested in seven nurses in whom antibody was no longer detectable gave positive stimulatio ... | 1985 | 2860346 |
| outbreaks of shingles. | | 1985 | 2865573 |
| an outbreak of shingles? | 7 of 101 staff within one department of a large office complex had shingles, as diagnosed by a general practitioner, within a three-month period. this incidence was significantly greater than that in the remaining workforce. varicella-zoster-specific igm antibody was detected in all 4 cases from whom early convalescent serum samples were obtained but in none of 22 controls. within the index department there was evidence of clustering in time and by work area. a case/control study showed that a r ... | 1985 | 2865576 |
| varicella zoster in the newborn. | | 1986 | 2870256 |
| effect of maternal varicella-zoster virus infection on the outcome of pregnancy and the analysis of transplacental virus transmission. | in result of 20 pregnancies complicated by varicella 11 healthy and 6 defective offspring were born, 2 pregnancies were aborted by the physician and one pregnancy terminated by stillbirth of 3 siblings. laboratory investigation of 16 pregnancies has shown that transplacental transfer of varicella-zoster (vz) virus did not occur in 13 cases (81.2%). in 3 cases transplacental transmission of vz virus was not inconsistent with the laboratory results, clinical course, and epidemiological analysis. i ... | 1986 | 2874730 |
| neonatal varicella infection. | | 1986 | 2878305 |
| focus assay for varicella-zoster virus in human embryo cells stained with immunoperoxidase method. | rapid titration of varicella-zoster virus (vzv) in human embryonic fibroblasts (hef) based on staining of virus-infected cells by indirect immunoperoxidase technique (ipa) is described. cell monolayers were grown in wells of plastic plates (two different diameters). foci of virus-infected cells as revealed by ipa could be counted either 48 hr post-infection, if cell-associated virus (vzv infected cells) was used as inoculum, or 72 hr p. i. if cell-free virus was used. a linear relationship was o ... | 1986 | 2881466 |
| prevention of varicella by vaccination. | | 1988 | 2896753 |
| identification and nucleotide sequence of the glycoprotein gb gene of equine herpesvirus 4. | the nucleotide sequence of the glycoprotein gb gene of equine herpesvirus 4 (ehv-4) was determined. the gene was located within a bamhi genomic library by a combination of southern and dot-blot hybridization with probes derived from the herpes simplex virus type 1 (hsv-1) gb dna sequence. the predominant portion of the coding sequences was mapped to a 2.95-kilobase bamhi-ecori subfragment at the left-hand end of bamhi-c. potential tata box, cat box, and mrna start site sequences and the translat ... | 1989 | 2915378 |