| autooxidation and hydroxylation reactions of oxygenated cytochrome p-450cam. | oxy-ferrous substrate-bound cytochrome p-450cam (mrso2) autooxidizes in the absence of its specific effector protein, putidaredoxin, without hydroxylating the substrate, camphor. the autooxidation is first order with an activation energy of 17 kcal mol-1 at 25 degrees, ph 7.0. substrate removal and low ph accelerate the reaction. the product, 5-exo-oh camphor, and a nonhydroxylated pseudosubstrate, norcamphor, stabilize the complex in a manner similar to camphor. increased oxidation rate of mrso ... | 1976 | 2601 |
| monomeric purine nucleoside phosphorylase from rabbit liver. purification and characterization. | rabbit liver purine nucleoside phosphorylase (purine nucleoside: orthophosphate ribosyltransferase ec 2.4.2.1.) was purified to homogeneity by column chromatography and ammonium sulfate fractionation. homogeneity was established by disc gel electrophoresis in presence and absence of sodium dodecyl sulfate, and isoelectric focusing. molecular weights of 46,000 and 39,000 were determined, respectively, by gel filtration and by sodium dodecyl sulfate-polyacrylamide disc gel electrophoresis. product ... | 1976 | 1390 |
| the effect of neonatal rat graft-vs-host disease (gvhd) on fc receptor lymphocytes. | the level of fc receptor rosette-forming lymphocytes (fc-rfl) was examined in spleen and lymph node cell suspension from neonatal da and bn rats inoculated within 24 hr of birth with either allogeneic l (experimental) or syngeneic (control) lymphoid cells. in addition, these levels were compared to fetal and neonatal animals that received no injection. the indicator cells (ea) were sheep erythrocytes sensitized with one-half concentration of the highest dilution of rabbit anti-sheep erythrocyte ... | 1976 | 1444 |
| the polysaccharide capsule of bacteroides fragilis subspecies fragilis: immunochemical and morphologic definition. | a large-molecular-weight capsular polysaccharide was isolated from strains of bacteroides fragilis subspecies fragilis. by means of electron microscopy and staining with ruthenium red, the thick polysaccharide capsule was also visualized. with use of a radioactive antigen-binding assay, antibody to this capsular polysaccharide was demonstrated in antisera prepared in rabbits to each of eight strains of b. fragilis fragilis. antibody of similar specificity was not found in antisera prepared to ba ... | 1976 | 1451 |
| the identification and analysis of the metabolic products of mephentermine. | phentermine (ib), n-hydroxymephentermine (ic) and n-hydroxyphentermine (id) were identified as metabolic products after in vitro incubation of mephentermine (ia) with rabbit liver microsomal fractions. compounds ia, ib and ic were also identified as excretion products in the urine of a human subject given a single dose of mephentermine (ia) sulphate. derivatization with acetic anhydride, trifluoroacetic anhydride and the trimethylsilyl donor reagent n,o-bis-(trimethylsilyl)-trifluoroacetamide (b ... | 1975 | 2665 |
| synthesis of angiotensin ii antagonists containing n- and o-methylated and other amino acid residues. | 1-n-methylisoasparagine,8-isoleucine- (i), 1-sarcosine,4-n-methyltyrosine,8-isoleucine- (ii), 1-sarcosine,5-n-methylisoleucine,8-isoleucine- (iii), 1-sarcosine,8-n-methylisoleucine- (iv), 1-sarcosine8k-n-methylisoleucine,8-n-methylisoleucine- (v), 1-sarcosine,8-o-methylthreonine- (vi), 1-sarcosine,8-methionine- (vii), and 1-sarcosine,8-serineangiotensin ii (viii), synthesized by merrifield's solid-phase procedure, possess respectively 0.8, 0.3, 0.5, 1.0, 0.0, 0.5, 3.7, and 0.7% pressor activity ... | 1976 | 2777 |
| the formation of cdp-diglyceride by isolated neuronal nuclei. | | 1975 | 1472 |
| treatment of ovarian hyperstimulation syndrome by antihistamine. | ovarian hyperstimulation was produced by human menopausal gonadotropin and chorionic gonadotropin in rabbits. a more rapid regression of the hyperstimualted ovaries was observed in an antihistamine-treated group than in a control group. the difference in regression was found to be statistically significant. the possibility of treating the ovarian hyperstimulation syndrome by antihistamine is cited. | 1976 | 1697 |
| h+ transport and membrane-bound hco - 3 atpase in salivary duct epithelium. | an atpase stimulated by hco - ions and other oxybases and inhibited by scn- has been found in main excretory duct of rat submaxillary gland, a tissue, capable of actively secreting hco - 3 ions. no such atpase was found in the rabbit duct, which normally does not secrete hco - 3. the hco - 3 atpase was localized in the plasma membrane fraction of the homogenate, as evidenced by the marker 5'-nucleotidase. the activities of the hco - 3 atpase increased in metabolic alkalosis and decreased in met ... | 1975 | 1708 |
| possibilities and limitations of uterine contraction inhibiting agents. | | 1976 | 2884 |
| animal phospholipases (author's transl). | | 1976 | 2906 |
| the influence of 5-hydroxytryptamine and some beta-adrenolytic compounds on adenylylcyclase in rabbit brain in vitro. | | 1975 | 1712 |
| the micro-organisms of tsetse flies. | micro-organisms from tsetse fly mycetomes were maintained in culture, where they were more pleomorphic than in the mycetomes, but were in some cases very similar to those observed in ovaries by other authors. agglutination tests on the cultured forms indicated in affinity to rickettsia. they were sensitive to antibiotics introduced by feeding flies on hosts treated with ampicillin; this reduced the longevity and fecundity of the tsetse flies and appeared to disturb normal digestion of bloodmeals ... | 1975 | 1986 |
| distribution of prostaglandin e 9-ketoreductase and nad+-dependent and nadp+-dependent 15-hydroxyprostaglandin dehydrogenase in the renal cortex and medulla of various species. | regional distributions of pge 9-ketoreductase and 15-hydroxy-prostaglandin dehydrogenase were examined in the cytoplasmic fractions from the kidneys of seven species. all species contained an nadph-dependent reductase, as well as nad+- and nadp+-dependent dehydrogenases in both cortex and medulla. a previously unrecognized cytoplasmic nadh-dependent pge 9-ketoreductase was also detected in the cortex and medulla of rat and bovine kidney. total nad+- and nadp+-dependent dehydrogenase activity was ... | 1975 | 2961 |
| effect of adsorbents on igm and igg measles antibodies. | sera from rabbits immunized with l-16 measles virus absorded with monkey blood cells; kaolin and blood cells; and mncl2 and heparin were examined in haemagglutination inhibition (hi) and neutralization tests. kaolin and mncl2 adsorbed primarily hi igm antibodies from the early immunization period. the adsorbents used had no influence on hi and neutralization igg antibodies. human convalescent serum gave similar results, i.e. only igg antibodies were found and they were not affected by kaolin and ... | 1975 | 1989 |
| serological differences between some isolates of bean yellow mosaic virus. | the degree of serological relatedness of three isolates of bean yellow mosaic virus (bymv) was studied. one common antigenic group (m) was demonstrated in all 3 isolates. in addition, another antigenic group (a) was demonstrated in the isolate fvm1. this group was absent from the isolates tpm3 and tpm11, in which the antigenic group n was found. antisera to the 3 isolates contained the corresponding antibody groups. the antibody group (m) shared by all 3 bymv isolates was present in the fvm1 ant ... | 1975 | 1992 |
| effect of ph on ionic exchange and function in rat and rabbit myocardium. | the effects of ph variation on ionic exchange and mechanical function were studied in the arterially perfused rat and rabbit septa. the ph and pco2 of the control perfusate were 7.40 and 39 mmhg, respectively. in the rabbit septum a metabolic acidosis (ph equals 6.82, pco2 equals 39 mmhg) caused a loss of 16% of control tension in 12 min. na+ and k+ exchange were unaltered. a comparable respiratory acidosis (ph equals 6.81, pco2 equals 159 mmhg) caused a 51% loss of tension in 2 min. na+ exchang ... | 1975 | 2014 |
| mechanisms of disposal of acid and alkali in rabbit duodenum. | stripped duodenal mucosa of rabbits was mounted in ussing chambers containing a ringer solution gassed with 100% o2. the disappearance of acid or alkali from the mucosal solution of short-circuited tissue was measured with a ph stat while the serosal ph was kept at 7.4. the duodenum rapidly disposed of both acid and alkali; neither property was altered by gassing with n2 while iodoacetate was in the perfusing solutions. prevention of release of co2 from the mucosal chamber obliterated the early ... | 1975 | 2019 |
| effect of acid lumen ph on potassium transport in renal cortical collecting tubules. | in order to determine the effect of acid lumen ph on renal tubular potassium transport, cortical collecting tubules were dissected from rabbit kidneys and perfused in vitro. when the ph of the perfusate was lowered from 7.4 to 6.8, potassium secretion into the tubule lumen decreased by an average of 47%. the transepithelial voltage increased from a mean value of -32 mv (lumen negative) at ph 7.4 to -51 mv at ph 6.8. net sodium absorption from the tubule lumen was essentially unchanged (5% mean d ... | 1976 | 3114 |
| use of protease inhibitor (trasylol) and heparin in cardiorespiratory resuscitation. ii. gasometric investigations of arterial blood. | the purpose of the present work was to assess the effect of trasylol--an inhibitor of proteases--and heparin on gasometric values in arterial blood during cardio-respiratory resuscitation. the investigations were carried out on rabbits in three experimental groups. in group i the simplest resuscitation procedures were applied: artificial respiration with ambu self-expanding bag and heart massage. in group ii oxygen was given for respiration, heart massage was applied, cardiac, vasoactive and alk ... | 1975 | 3120 |
| heat resistance of ileal loop reactive bacillus cereus strains isolated from commercially canned food. | sporeformers isolated from a commercially canned food were identified as bacillus cereus, lactose-positive variants. the thermal resistance of spore crops produced from each of two representative cultures was determined in 0.067 m phosphate buffer at ph 7.0. the d121.1 values for one isolate were approximately 0.03 min (z = 9.9c), whereas the d121.1 values for the other isolate were 2.35 min (z = 7.9 c). thermal inactivation results for heat-stressed isolates from each strain showed no significa ... | 1975 | 2108 |
| purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland. | 1. 6-phosphogluconate dehydrogenase from rabbit mammary gland was purified to homogeneity by the criterion of polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. the molecular weight of the subunit is 52 000. the enzyme was purified 150-fold with a final specific activity of 20 mumol of nadp+ reduced/min per mg of protein and overall yield of 3%. the molecular weight of the native enzyme is estimated to be 104 000 from gel-filtration studies. the final purification ste ... | 1975 | 3163 |
| pharmacological properties of 2-(2-chloro-p-toluidino)-2-imidazoline-nitrate (tolonidine), a new antihypertensive agent. iii. action on the secretions of the digestive tract and on the central nervous system, acute toxicity. | the pharmacological properties of 2-(2-chloro-p-toluidino)-2-imidazoline-nitrate (tolonidine) a new synthetic derivative of imidazoline are reported in a series of three successive articles. this compound has been shown to possess hypotensive and antihypertensive properties. after i.v. administration, the hypotensive phase was preceded by hypertension related to the potent direct alpha-sympatheticomimetic properties of the product. this pressor response, which was not seen after oral administrat ... | 1975 | 3186 |
| adrenergic drug-receptor interaction in the presence of strontium (sr++) in mammalian myocardium. | the specificity of ca++ for the interaction of beta adrenergic agonists with their receptors in rabbit right atrial muscle was evaluated. this was accomplished by substituting ca++ by an equimolar concentration of sr++. dose-response curves which demonstrate the effect of norepinephrine and isoproterenol on the rate of electrical activity in the presence of ca++ or sr++ were made. in addition, the antagonistic action of propranolol (1 x 10(-7) m) in a ca++-containing or sr++-containing medium wa ... | 1975 | 2115 |
| purification and characterization of a collagenase extracted from rabbit tumours. | a collagenase was purified from homogenates of v2 ascites-cell carcinoma growing in rabbit muscle. (nh4)2so4 precipitation, ion-exchange and gel-filtration chromatography, and affinity chromatography (by using the cb7 cnbr) cleavage fragment of alpha 1(i) collagen linked to agarose) gave a 268000-fold purification and a sevenfold increase in total enzyme units recovered. the specific activity, defined as mumol of collagen in solution cleaved/h per mg of enzyme at 35 degrees c, was 1.74.2. the co ... | 1975 | 2161 |
| quantitative localization of tyrosine hydroxylase, dopamine-beta-hydroxylase, phenolethanolamine-n-methyl transferase, and glutamic acid decarboxylase in spinal cord. | sensitive radiometric assays for tyrosine hydroxylase (th), dopamine-beta-hydroxylase (dbh), phenylethanolamine-n-methyl transferase (pnmt), and glutamic acid decarboxylase (gad) have been combined with microdissection techniques for quantitative localization of these synthetic enzymes in rabbit spinal cord. th was present uniformly in gray and white matter. dbh was higher in the lateral horns than in the other gray matter areas, and was not detectable in white matter. pnmt was detectable in gra ... | 1976 | 3266 |
| rabbit erythrocyte membrane interaction with promazine and calcium. | | 1975 | 2177 |
| chromatin-bound protease: degradation of chromosomal proteins under chromatin dissociation conditions. | a chromatin-bound protease, active in 2 m nacl-5 m urea or 5 m urea alone, was demonstrated in rat liver, kidney, testes, brain, rabbit bone marrow, chicken reticulocyte, and ehrlich ascites chromatin. chicken erythrocyte chromatin did not possess any detectable proteolytic activity in salt and urea. the proteolytic activity of rat liver chromatin in salt and urea was found to be independent of the methods of chromatin preparation. the protease can be inhibited by the serine specific reagents ph ... | 1976 | 2280 |
| methodological simplifications in radioimmunoassay of urinary aldosterone. | simplification of radioimmunoassay procedures of urinary aldosterone-18-glucuronide was attempted, taking into consideration the aspects implied by the hydrolysis of urine and the assay itself. the procedure standardized for the hydrolysis step (samples diluted with a two-fold volume of 0.2 n hcl and incubated at 30 degrees c for 16-24 h) proved suitable in terms of practicability and accuracy. aldosterone antisera, raised in the rabbit against an aldosterone-3-bovine albumin conjugate, were sel ... | 1976 | 2397 |
| multiple changes in distal stop-flow electrolyte patterns and reduction of acid excretion induced in rabbits by angiotensin. | 1. angiotensin has previously been shown to inhibit distal renal tubular sodium reabsorption. as a consequence of this, or independently, it might influence the distal handling of other electrolytes. we have therefore examined the effects of angiotensin on the distal reabsorption or secretion of a spectrum of electrolytes. 2. standard bilateral stop-flow studies were done on anaesthetized, adrenalectomized rabbits, in which the effects of intravenous infusions of either 0-02-0-05 mug min-1 kg-1 ... | 1976 | 3304 |
| characteristics of lipopolysaccharides of salmonella typhi isolated from carriers and patients suffering from typhoid fever. | lipopolysaccharides (lps) of salmonella typhi strains, isolated from carriers and patients suffering from typhoid fever, were characterised according to their biochemical properties, morphological structure and degree of aggregation of complexes. all preparations of lps, regardless of their origin, were morphologically heterogeneous. free electrophoresis and immunoelectrophoresis demonstrated that lps preparations were composed of components possessing different mobilities in electric fields. lp ... | 1976 | 2523 |
| oxidative deamination of biogenic amines by intestinal amine oxidases: histamine is specifically inactivated by diamine oxidase. | the ability of the gut to inactivate various amines by oxidative deamination was tested with a 130-fold purified amine oxidase preparation from dog small intestine. of 34 amines tested, putrescine, benzylamine, cadaverine, and serotonin were the most favourable substrates. histamine was inactivated rapidly by this enzyme preparation, too. histamine derivatives methylated at the imidazole nucleus were also deaminated, whereas nalpha-methylhistamine was only a poor substrate and nalpha, nalpha-dim ... | 1975 | 2528 |
| purification and properties of drosophila virilis texmelucan l-glycerol-3-phosphate dehydrogenase. | | 1976 | 3351 |
| the binding of catecholamines to the serum proteins of the rat and the domestic fowl. | | 1975 | 3370 |
| postnatal development of mixed-function oxidation as measured in microsomes from the small intestine and liver of rabbits. | the postnatal development of aminopyrine n-demethylase, aniline 4-hydroxylase, benzpyrene hydroxylase, biphenyl 4-hydroxylase, 7-ethoxycoumarin 0-deethylase activities, nadph-cytochrome c reductase, and cytochrome p-450 was compared in microsomes from the liver and small intestine of new zealand white rabbits. apart from hepatic aniline hydroxylase activity, all of the xenobiotic-metabolizing enzyme activities examined had a similar pattern of development in the liver and small intestine. in bot ... | 1976 | 3396 |
| the possibilities and limitations of membrane methods for the histochemical demonstration of cholinesterases. | the thiocholine method for the histochemical detection of cholinesterases according to karnovsky-roots was adapted for unfixed cryostat sections by addition of the agar solution to the incubation mixture and by using the semipermeable membrane interposed between the section and the incubation medium. the procedure prevents the leakage of the enzyme activity of the section and is suitable for tissues where the cholinesterase activity is low. | 1976 | 2569 |
| the reconstruction of enzymic lipid peroxidation systems from microsomes of various origin. | | 1976 | 3437 |
| identification of albumin as the serum factor essential for the growth of activated human lymphocytes. | albumin from human, bovine, or rabbit serum supported the growth of concanavalin a-stimulated human thymus-derived lymphocytes equally well. this activity was completely abolished by pepsin digestion. it was shown for bovine serum albumin that the albumin molecule itself, and neither an impurity nor a factor bound to albumin was essential for the growth of lymphocytes. this conclusion was based on observations that the growth-promoting activity could not be removed from albumin, and that the spe ... | 1976 | 2610 |
| immunochemical relationships between bacteria belonging to two separate families: pneumococci and klebsiella. | | 1976 | 3470 |
| a doppler assay for the antigen--antibody reaction. | the specific combination of antigen with antibody was detected rapidly and with high sensitivity by an assay based on particle electrophoresis and laser light scattering. antibody attachment to dilute suspensions of submicroscopic polystyrene spheres coated with antigen was detected as changes in the doppler shift of scattered laser light when the suspended particles were subjected to an electric field. antibody or antigen concentrations in the range of 5--10 nanograms per ml can be detected in ... | 1976 | 3612 |
| a rabbit reticulocyte model for the role of hemin-controlled repressor in hypochromic anemias. | hemin allows maximal protein synthesis in intact rabbit reticulocytes and their cell-free lysate preparations by retarding the formation of a translational repressor (hcr) found in the postribosomal supernate. in order to evaluate the role of hcr in the pathogenesis of hypochromic anemias, hcr was isolated and partially purified from intact rabbit reticulocytes incubated in vitro with either 0.1 mm alpha,alpha-dipyridyl (an iron-chelating agent) or 0.1 m ethanol. both of these agents inhibit ret ... | 1976 | 2617 |
| cornea preparation for in vitro bio-pharmaceutical evaluation of ophthalmic dosage forms. | a system has been developed for a specific biopharmaceutical purpose: testing for ophthalmic preparations, the influence of formulation on drug transport through the cornea. the apparatus is a lucite cell, divided in two compartments by a clamped rabbit cornea. physiological conditions are ensured by a supply of oxygenated perfusion medium. they are monitored by electrical conductivity and corneal thickness measurements. reliability of the system was tested in a set of experiments. | 1975 | 2663 |
| immobilized glucuronosyltransferase for the synthesis of conjugates. | partially purified rabbit liver udpglucuronosyltransferase is immobilized on agarose by the cyanogen bromide activation method. both soluble and matrix-bound enzyme preparations display very similar km and ph optimum. the storage stability of the immobilized enzyme at 4 degrees is 5-10 times improved over the soluble preparations. the agarose-bound udpglucuronosyltransferase is successfully used in the synthesis of p-nitrophenyl glucuronide in an overall yield of 50-70%. the matrix-bound enzyme ... | 1976 | 2779 |
| proline endopeptidase and exopeptidase activity in polymorphonuclear granulocytes. | peptidases capable of releasing proline residues from polypeptides are present in the cytoplasmic fraction of rabbit polymorphonuclear granulocytes. this was shown with peptide substrates where proline is present either at the carboxy-terminal or within the polypeptide chain. lysosomal and plasma membrane enzymes were inactive towards such polypeptides. the proline residue was hydrolyzed at either its amino end or its carboxy end. it is noteworthy that a pro:pro bond was cleaved both in the pent ... | 1976 | 3727 |
| non-barbiturate intravenous anesthetics. | | 1976 | 3750 |
| factors influencing nonspecific binding of glucocorticoids in myocardial tissue. | myocardial slices from the left ventricle of cat hearts were incubated in krebs-henseleit buffer containing 10 mm glucose which were gassed with 95% o2 and 5% co2. tritiated dexamethasone (dexa 0.171 mm) or methylprednisolone (mp 0.805 mm) was added under varying conditions of temperature, ph, and in the presence of various metabolic inhibitors. glucocorticoid uptake by myocardial tissue was found to be temperature-dependent, plateauting after 60 min of incubation at 0.21 mumole of dexa/g of tis ... | 1976 | 3798 |
| sea wasp toxoid: an immunizing agent against the venom of the box jelly fish, chironex fleckeri. | | 1975 | 3866 |
| lactic acidosis: an experimental model. | a model of spontaneous lactic acidosis was developed in alloxan diabetic rabbits by infusing intravenously beta-hydroxybutyric acid followed by a continuous infusion of nahco3. in half of the animals, the arterial lactate/pyruvate ratio rose from 2.5 mm/0.19mm to 20.4 mm/0.28 mm, and arterial ph fell to 7.16. in animals with lactic acidosis, the calculated ratio in blood of nad/nadh was 1437 +/- 230, versus a normal value of 6754 +/- 1250. both arterial po2 and blood pressure were normal. contin ... | 1976 | 2836 |
| diamine oxydase in rabbit small intestine: separations from a soluble monoamine oxidase, properties and pathophysiological significance in intestinal ischemia. | from all mammals investigated so far only in rabbits diamine oxidase could not be detected in any tissue except the gut. thus this species was chosen for studying the physiological and pathophysiological function of this enzyme in the gastrointestinal tract. by gel filtration on sephadex g 50 and g 200 the enzyme was purified 100-fold, separated from a soluble monoamine oxidase, and the properties of the two enzymes were determined. diamine oxidase from rabbit small intestine deaminated putrecin ... | 1975 | 3954 |
| are the pial arterial responses dependent on the direct effect of intravascular pressure and extravascular and intravascular po2, pco2, and ph? | | 1975 | 2837 |
| studies on the anoxic inhibition of myocardial protein synthesis. | the present study demonstrates that exposure of cardiac muscle to high levels of glucose during anoxia appears to retard damage to myocardial protein synthesis. the mechanism of this "glucose" effect is glucose-specific and appears related to the intracellular metabolism of glucose by the anoxic myocardium. | 1975 | 2956 |
| ecologic studies of venezuelan encephalitis virus and isolations of nepuyo and patois viruses during 1968-1973 at a marsh habitat near the epicenter of the 1969 outbreak in guatemala. | ecologic studies of venezuelan encephalitis (ve) virus at a marsh habitat near the epicenter of the 1969 outbreak in guatemala revealed that the virus was enzootic there. ve virus was isolated yearly during 1968-1973 from sentinel hamsters exposed during the rainy seasons and from mosquitoes collected during july and august 1970. hamsters yielded 41 strains of ve virus and virus was detected within 2 km of the edge of the marsh, in its interior, and at its western extreme 18 km from the central ... | 1976 | 3981 |
| interaction of hemopexin with water-soluble porphyrins. | | 1976 | 4020 |
| nadh-dependent o-deethylation of p-nitrophenetole with rabbit liver microsomes. | | 1976 | 4027 |
| phosphatidate phosphohydrolase and palmitoyl coenzyme a hydrolase in cardiac subcellular fractions. | the characteristics of the two enzymes related to fatty acid esterifaction were studied in order to provide fundamental information leading to further understanding of the control of myocardial glyceride formation. palmitoyl-coa hydrolase and phosphatidate phosphohydrolase are both distributed unevenly among heart subcellular fractions. the activity of the latter enzyme in subcellular fractions changes independently in response to mg2+ addition and in response to thyroid hormone treatment of ani ... | 1975 | 2957 |
| resolution, purification and characterization of rabbit serum atropinesterase and cocainesterase. | | 1976 | 4075 |
| hepatic and extrahepatic metabolism, in vitro, of an epoxide (8-(14) c-styrene oxide) in the rabbit. | | 1976 | 4076 |
| the function of shuttle systems of liver extramitochondrial hydrogen transport in experimental atherosclerosis. | an enzymatic activity of shuttle systems for reduction equivalents transport (malate-aspartate, glycerophosphate, lactate, glutamate and beta-hydroxybutyrate dehydrogenase) was studied spectrophotometrically in liver tissue of intact rabbits and animals with experimental atherosclerosis. content of malate, oxaloacetate, glutamate, alpha-ketoglutarate, alpha-glycerophosphate, dihydroacetone phosphate, lactate, pyruvate, beta-hydroxybutyrate and acetoacetate were studied. in experimental atheroscl ... | 1975 | 3020 |
| isolation and properties of 2 kallikreins from human saliva. | two kallikreins (k-i and k-ii) were purified from mixed, parotid and submandibular human saliva. the kallikreins were separated by chromatography on cm-cellulose. the ph optima of activity were at ph 9.3 and ph 9.6-9.8; km for baee was 1.10-3m and 4.10-3m, respectively. the esterase activity of k-i and k-ii was inhibited by trasylol-like inhibitors, while the plant and synthetic inhibitors of trypsin were uneffective. in dog, rabbit and rat the hypotensive effect of k-ii and its action upon the ... | 1975 | 3025 |
| the correlation between activity of enzymes participating in the formation and utilization of acetyl coa in rabbit heart mitochondria in myocarditis and normal state. | activities in rabbit heart mitochondria of acetoacetyl-coa-thyolase, pyruvate dehydrogenase, acetyl coa-synthetase, citrate synthase and acetyl carnitine transferase were compared. these enzymes participate in formation and utilization of acetyl-coa. the acetoacetyl-coa-thyolase and acetyl coa-synthetase were shown to possess the more distinct capacity in vitro to form acetyl coa. the co-enzyme was most efficiently utilized under these experimental conditions by the citrate synthase. the enzymes ... | 1975 | 3026 |
| enzyme histochemical studies of rabbit bile ducts with and without bile flow. | the histochemical enzyme pattern in normal, extra-hepatic bile ducts from rabbits was studied. a difference between the duct epithelium and the crypt epithelium was noted mainly in the activity of gamma-glutamyl-transpeptidase and alkaline phosphatase which only stained positively in the crypts. no difference from the normal enzyme pattern was noted after diversion of the bile flow for up to 30 days. | 1976 | 3088 |
| a comparative study on the irreversible binding of labeled halothane trichlorofluoromethane, chloroform, and carbon tetrachloride to hepatic protein and lipids in vitro and in vivo. | 1) after intraperitoneal injection of labeled ccl4, chcl3, and halothane in mice, 14c is preferentially bound to liver endoplasmic protein and lipid. a considerable activity is also associated with mitochondrial constituents. maximal protein binding (nmol/mg): ccl4: 2.8 (0.5 hrs); chcl3: 11.5 (6 hrs); halothane: 5 (6 hrs). lipid binding: ccl4: 6.4 (5 min); chcl3: 8 (4 hrs); halothane: 13.5 (2 hrs). the form of the binding curves in microsomal and mitochondrial protein and lipid differed with the ... | 1975 | 3152 |
| studies on gamma-glutamyl transpeptidase in human and rabbit erythrocytes. | gamma-glutamyl transpeptidase transfers the gamma-glutamyl moiety of glutathione to a variety of acceptor amino acids. through the operation of the gamma-glutamyl-cyclotransferase cycle, this enzyme has been implicated in the transport of amino acids into cells, especially the cells of the proximal tubules of kidney. it has been reported to be present in rabbit erythrocytes. however, using white cell-free preparations, we have not been able to demonstrate the presence of gamma-glutamyl transpept ... | 1976 | 4175 |
| effect of sas (a new 10-n-acylaminophenothiazine) on gastric secretion and ulceration in rats. | the antiulcer and antisecretory activity of 2-chloro-10-4'(n-beta-hydroxyethyl) piperazinyl-1' acetylphenothiazine (sas) has been investigated. at 10 and 20 mg/kg (s.c.) the drug was found to possess potent antigastric secretory and antiulcer activity (both in shay and stress ulcers) and did not exhibit any peripheral parasympathetic blocking activity. the pronounced antispasmodic activity of sas was nonspecific rather than a specific parasympatholytic effect. | 1976 | 4186 |
| amino acids attached to transfer ribonucleic acid in vivo. | 1. trna was extracted from rabbit liver by both the phenol and diethyl pyrocarbonate methods under conditions preventing deacylation of the amino acids attached in vivo. 2. after deacylation 12 amino acids were determined by gas-liquid chromatography, by using the flame-ionization and nitrogen-sensitive thermionic detectors. 3. comparison of the distribution of 12 amino acids attached to trna with those contained in total tissue protein and in the free pool showed little correlation. 4. results ... | 1975 | 2156 |
| subtilopeptidase a isoenzyme system. interaction with serum components and its importance for quantitative immunoelectrophoresis. | a method was developed which involved electroimmunoassay and crossed immunoelectrophoresis of subtilopeptidase a (ec 3.4.21.14). initial trials with unfractionated antiserum were not successful and interaction of the enzyme with non-immunoglobulin serum components were shown to be the cause of the failures. quantitative immunoelectrophoresis was possible when purified immunoglobulins were used. a ph of 6.5 (lower than the usual ph 8.6) was necessary to obtain a proper baseline definition. subtil ... | 1975 | 2159 |
| hypothalamic inactivation of thyrotrophin-releasing hormone. | following the demonstration of peptidases in the rat hypothalamus which inactivate thyrotrophin-releasing hormone (trh), a sensitive and specific radioimmunoassay for the releasing hormone was used to investigate the presence of similar peptidases in the rabbit hypothalamus. trh was found to be rapidly inactivated by supernatant and particulate hypothalamic fractions, with higher peptidase activity in the supernatant than in the particulate fraction. an optimum ph of 7.3 within physiological lim ... | 1976 | 4345 |
| humoral pressor and depressor factors in vibration disease. | | 1976 | 4357 |
| fructose 1,6-diphosphate aldolase from rabbit muscle. effect of ph on the rate of formation and on the equilibrium concentration of the carbanion intermediate. | the rate of oxidation of ferricyanide of the aldolase-dihydroxyacetone phosphate complex was measured under different conditions. the following conclusions are drawn. 1. in the cleavage of fructose diphosphate, catalysed by native aldolase, the steady-state concentration of the enzyme-dihydroxyacetone phosphate carbanion intermediate represents less than 6% of the total enzyme-substrate intermediates. 2. fructose diphosphate and dihydroxyacetone phosphate compete for the four catalytic sites on ... | 1975 | 2160 |
| extraction of a calcium-phospholipid-phosphate complex from bone. | a calcium-phospholipid-phosphate complex with a constant 1:1 calcium to total phosphate molar ratio is shown to exist in rabbit and calf bone. this complex, which may be involved in the transport and deposition of bone mineral, appears to constitute a significantly greater proportion of the lipids of younger bone than of more mature bone. the comples was isolated by a modified folch extraction employing ultrasonic disruption of cellular material. evidence is presented to show that the complex is ... | 1976 | 3268 |
| effects of hypoxia on distribution of cardiac output and organ blood flow in the rabbit. regional vascular response to hypoxia. | the hemodynamic responses of various vascular beds in the systemic circulation to prolonged moderate hypoxia were studied in the rabbit using the radioactive microsphere method. although cardiac output remained unchanged, there was a redistribution of blood flow in which blood was mainly diverted from the kidneys to provide greater supply to heart, brain and skeletal muscle. these regional adjustments are similar to those seen after low cardiac output due to hemorrhage or endotoxic shock. | 1975 | 3281 |
| comparison of the effects of depolarizing agents and neurotransmitters on regional cns cyclic gmp levels in various animals. | | 1976 | 4583 |
| transformation and excretion of drugs in biological systems. xi. renal excretion mechanisms of sulfonamides in rabbits. | | 1975 | 3289 |
| the effect of sodium taurocholate on the permeability of antral and fundic gastric mucosa. | | 1976 | 4654 |
| pituitary testicular relationship with adult male rabbit after experimental cryptorchidism. | | 1976 | 4656 |
| surface immunoglobulins on xenopus laevis lymphocytes. | | 1976 | 3324 |
| the electrophysiological characteristics of glucose absorption of the goldfish intestine as compared to mammalian intestines. | | 1976 | 3331 |
| n-acetylation of drugs. pharmacogenetic studies in rabbits selected for their acetylator characteristics. | studies on acetylation of sulfadiazine, isoniazid, and p-aminobenzoic acid in selected lines of slow and rapid acetylator rabbits are described. pedigree analysis of rabbits classified as slow or rapid sulfadiazine acetylators confirmed previous studies that the rate of sulfadiazine elimination (acetylation) is genetically controlled, with rapid elimination dominant over slow elimination of the drug. pharmacokinetic studies in rabbits of specified sulfadiazine acetylator genotypes with isoniazid ... | 1976 | 3408 |
| the effects of some polypeptides on the systemic blood pressure of sheep (author's transl). | | 1975 | 3458 |
| activation and desensitization of platelets by platelet-activating factor (paf) derived from ige-sensitized basophils. i. characteristics of the secretory response. | the secretion of vasoactive amines from rabbit platelets induced by the platelet-activating factor (paf) derived from ige-sensitized rabbit basophils, was examined. the secretion required calcium has previously been shown to be noncytotoxic and was optimal in both rate and extent at 37 degrees c and ph 7.2. different temperature-sensitive steps were rate limiting for secretion above or below 20 degrees c. the rate of secretion was dependent upon the concentration of paf and also of platelets. ma ... | 1976 | 3618 |
| activation of platelets by platelet-activating factor (paf) derived from ige-sensitized basophils. ii. the role of serine proteases, cyclic nucleotides, and contractile elements in paf-induced secretion. | secretion of serotonin from platelets induced by platelet-activating factor (paf) derived from antigen-stimulated, ige-sensitized rabbit basophils was studied to further characterize the biochemical requirements. inhibition of secretion with diisopropylphosphofluoridate (dfp) was observed if the dfp was present during the reaction, but not if platelets or paf were pretreated with the inhibitor. this suggested a role for an activatable serine protease in the secretion. supporting evidence came fr ... | 1976 | 3619 |
| studies of the histidine residues of triose phosphate isomerase by proton magnetic resonance and x-ray crystallography. | | 1976 | 3655 |
| new concepts on the pathogenesis of atherosclerosis and its practical aspects. | | 1976 | 4928 |
| pharmacological action of triazolam. | | 1975 | 4945 |
| hormones, second messengers, and the mammalian spermatozoon. | | 1975 | 4967 |
| proceedings: the eeg effects of vufb-10.032, clozapine and octoclothepin in rabbits. | | 1975 | 3932 |
| evidence for dimethyltryptamine (dmt) as a naturally-occurring transmitter in mammalian brain. | | 1976 | 4987 |
| toxicological characteristics of ampicillin. | toxocity of ampicillin trihydrate was studied in acute and chronic experiments. it was shown that the antibiotic had low acute toxicity, did not cumulate and had no skin-irritating effect. on its inhalation in concentrations of 5 mg/m3 for 4 months, ampicillin induced allergization of albino rats, decreased their immunity. the general toxic effect of the drug was slightly pronounced. ampicillin in a concentration of 0.1 mg/m3 induced tension of the immunological reactivity of the organism. the m ... | 1975 | 5051 |
| gamma-glutamyl transpeptidase in the urine from an isolated rabbit kidney perfused with and without dmso. | | 1976 | 5095 |
| activation of the o-glucuronide of the carcinogen n-hydroxy-n-2-fluorenylacetamide by enzymatic deacetylation in vitro: formation of fluorenylamine-trna adducts. | | 1976 | 5097 |
| isolation of brain endopeptidases: influence of size and sequence of substrates structurally related to bradykinin. | two thiol-activated endopeptidases with ph optima near ph 7.5 were isolated from the supernatant fraction of rabbit brain homogenates by deae-cellulose chromatography, gel filtration and isoelectrofocusing. peptide bond hydrolysis was measured quantitatively by ion-exchange chromatography with an amino acid analyzer. brain kininase a hydrolyzes the phe5-ser6 peptide bond in bradykinin (bk), arg1-pro2-pro3-gly4-phe5-ser6-pro7-phe8-arg9. it is isoelectric near ph 5.2 and has a molecular weight of ... | 1976 | 5120 |
| effects of salicylate and bile salt on ion transport by isolated gastric mucosa of the rabbit. | the effects of luminal addition of salicylate and taurocholate on ion transport by fundic mucosa were examined in vitro using isotopic and ph stat techniques. salicylate, 3 mm, did not alter, but 20 mm caused a 40% decrease in, the acid secretory rate. taurocholate, 20 mm, caused a transient, apparent cessation of acid secretion followed by stimulation. salicylate, 3 or 20 mm, increased na+ but not cl- permeability at luminal ph 7. at luminal ph 4, however, salicylate increased cl- in addition t ... | 1976 | 3973 |
| erythropoietin production after renal denervation or beta-adrenergic blockade. | the ability of either beta-adrenergic blockade or bilateral renal denervation to alter erythropoietin (esf) production in rabbits exposed to hypobaric hypoxia was studied. esf elaboration during 5 h of exposure to hypoxia was not affected by beta-blockade, but was markedly reduced by prior surgical denervation of both kidneys. after 18 h of hypoxia plasma esf levels in renal denervated rabbits did not differ significantly from those of sham-operated controls. previous studies have shown that esf ... | 1976 | 3977 |
| adsorption of glyceraldehyde 3-phosphate dehydrogenase on condensed monolayers of phospholipid. | the adsorption of 14c alkylated glyceraldehyde 3-phosphate dehydrogenase from rabbit muscle to condensed monolayers of phosphatidic acid was investigated under a variety of conditions. 2. the rate constant for association at 20 degrees c depended on ionic strength. at i/2=60mm the rate constant was 0.39min-1. at i/2=260mm it decreased to 0.27min-1. 3. the apparent association constant (kass.) for adsorption at i/2=60mm was 1.06 x 10(6)m-1 and was strongly influenced by subphase changes in ph and ... | 1976 | 4058 |
| isozymes of amp deaminase in red and white skeletal muscles. | | 1975 | 5297 |
| an analysis of the autophosphorylation of rabbit and human erythrocyte membranes. | the autophosphorylation of rabbit and human erythrocyte membranes has been studied under various experimental conditions. the phosphopeptides of the erythocyte membranes were identified using sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis followed by ratioautography. the pattern of phosphorylatiion of membrane components differs with respect to the phosphoryl donor used (atp or gtp) and to the ph at which the reaction is carried out. both species appear to contain at least two di ... | 1976 | 4093 |
| purification of multiple forms of adenosine deaminase from rabbit intestine. | two forms of adenosine deaminase (adenosine aminohydrolase, ec 3.5.4.4), differing in molecular size, have been purified and obtained in homogeneous form from rabbit intestine. the purification procedures involved extraction with acetate buffer, ph 5.5, precipitation and fractional reextraction with (nh4)2so4, ion-exchange chromatography on deae-cellulose and gel filtration on sephadex g-75 and sephadex g-200. gel filtrations analysis gave molecular weight estimates of 265 000 and 32 000 for the ... | 1976 | 4139 |
| proacrosin from rabbit epididymal spermatozoa: partial purification and initial biochemical characterization. | | 1975 | 4165 |
| adjuvant and immunostimulating activities of water-soluble substances extracted from mycobacterium tuberculosis (var. hominis). | water-soluble substances have been extracted from two strains of mycobacterium tuberculosis var. hominis: the native hydrosoluble part (polysaccharide and peptidoglycan), a substance in which the polysaccharide moiety is less abundant than in the latter, the acetylated peptidoglycan and, finally a tetrasaccharide-heptapeptide. all four types of substances, when they were injected together with freund's incomplete adjuvant, exerted an adjuvant effect on the production of delayed-type hypersensiti ... | 1975 | 4166 |
| the gas chromatographic mass spectrometric determination of trifluoroacetic acid in biological fluid. application to halothane metabolism. | the methyl ester of trifluoroacetic acid was prepared by reaction with n,n'-dimethylformamide dimethylacetal and was successfully passed through a gas chromatograph. trifluoroacetic acid was detected by the use of gas chromatography and low and high resolution gas chromatography mass spectrometry in an acidic extract of an incubation medium containing microsomes, reduced nicotinamide adenine dinucleotide phosphate, oxygen and halothane. however, trifluoroacetic acid could not be detected when ni ... | 1976 | 4173 |