| formation of homogeneous carbohydrate-lectin cross-linked precipitates from mixtures of d-galactose/n-acetyl-d-galactosamine-specific lectins and multiantennary galactosyl carbohydrates. | quantitative precipitation studies have shown that the man/glc-specific lectin concanavalin a (cona) forms homogeneous (homopolymeric) cross-linked precipitates with individual asparagine-linked oligomannose and bisected hybrid-type glycopeptides in the presence of binary mixtures of the carbohydrates [bhattacharyya, l., khan, m. i. & brewer, c. f. (1988) biochemistry 27, 8762-8767]. the results indicate that the cona-glycopeptide precipitates are highly organized cross-linked lattices that are ... | 1992 | 1511686 |
| analysis of dna sequences transcribed at high levels in bradyrhizobium japonicum bacteroids but not necessary for symbiotic effectiveness. | five regions of the bradyrhizobium japonicum genome, which are transcribed at high levels in nitrogen-fixing soybean (glycine max) nodules, were identified. none of these regions contained previously identified genes (e.g., nif, nod, and fix genes) that are known to be essential for development of functional nitrogen-fixing nodules. to assess the role of these regions in the development of the b. japonicum-soybean symbiosis, we cloned and used them to construct b. japonicum strains, in which lar ... | 1992 | 1515666 |
| effect of lectins on hepatic clearance and killing of candida albicans by the isolated perfused mouse liver. | the isolated perfused mouse liver model was used to study the effects of various lectins on hepatic trapping and killing of candida albicans. after mouse livers were washed with 20 to 30 ml of perfusion buffer, 10(6) c. albicans cfu were infused into the livers. at the time of recovery, 63% +/- 2% (mean +/- standard error of the mean) of the infused c. albicans cfu were recovered from the liver and 14% +/- 1% were recovered from the effluent for a total recovery of 77% +/- 2%. this indicated tha ... | 1992 | 1541519 |
| apparent digestibility of amino acids in raw and heated conventional and low-trypsin-inhibitor soybeans for pigs. | an experiment was conducted with ileally cannulated pigs to determine the apparent digestibility of amino acids and n in raw or heated conventional or low-trypsin-inhibitor soybeans. six littermate barrows initially averaging 24 kg were fed cornstarch-based diets (10.5% cp, .68% lysine, 3,558 kcal of me/kg) supplemented with raw (unheated), conventional soybeans; raw, low-trypsin-inhibitor soybeans; heated, conventional soybeans; heated, low-trypsin-inhibitor soybeans; or solvent-extracted soybe ... | 1992 | 1564006 |
| chemical, in vitro, and in vivo evaluation of soybeans heat-treated by various processing methods. | in trial 1, eight holstein heifers weighing 410 kg were used in an 8 x 8 latin square and fed tmr containing 79.3% alfalfa silage and 20% soybeans. the first four treatments were raw soybeans, soybeans roasted and held for 3 h at the roasting temperature, extruded soybeans, and soybeans roasted in a california pellet mill jet-sploder. the remaining four treatments were obtained by altering the residence time of soybeans in the jet sploder. the temperatures of soybeans exiting the roaster were 11 ... | 1992 | 1569269 |
| biochemical and immunological properties of alkaline invertase isolated from sprouting soybean hypocotyls. | alkaline invertase from sprouting soybean (glycine max) hypocotyls was purified to apparent electrophoretic homogeneity by consecutive use of deae-cellulose, green 19 dye, and cibacron blue 3ga dye affinity chromatography. this protocol produced about a 100-fold purification with about a 11% yield. the purified protein had a specific activity of 48 mumol of glucose produced mg-1 protein min-1 (ph 7.0) and showed a single protein band in sodium dodecyl sulfate-polyacrylamide gel electrophoresis ( ... | 1992 | 1575518 |
| proteins that rescue a-signal-defective mutants of myxococcus xanthus. | the asg mutants of myxococcus xanthus are defective in the production of an extracellular substance, called a-factor, that is required for expression of a set of fruiting body-specific genes. a-factor is released by wild-type cells (asg+) after 1 to 2 h of development. when a-factor is added to asg mutant cells, it restores expression of their a-factor-dependent genes. rescue of beta-galactosidase production in an asg mutant carrying the a-factor-dependent lacz transcriptional fusion (omega 4521 ... | 1992 | 1577696 |
| intravenous thyrotropin (tsh)-releasing hormone releases human tsh that is structurally different from basal tsh. | to determine whether basal tsh differed structurally from trh-released tsh, the tsh obtained from 11 normal subjects before and after the iv administration of trh was characterized using lectin-affinity chromatography. tsh was applied to the following lectins: lentil, ricin (both before and after tsh treatment with neuraminidase), concanavalin-a, wheat germ, glycine max, helix pomatia, dolichos biflorus, arachis hypogaea, and vicia villosa (isolectin b4). after each column was washed to elute un ... | 1992 | 1592875 |
| changes in serum and urinary uric acid levels in normal human subjects fed purine-rich foods containing different amounts of adenine and hypoxanthine. | the effect of ingesting some purine-rich foods (beef liver, haddock fillets and soybeans) on uric acid metabolism was investigated in 18 male subjects with no history of gout or kidney disorder. in a crossover design, three isoenergetic and isonitrogenous meals were fed to volunteers during a 3-week period. only the content of uricogenic bases (adenine and hypoxanthine) varied among the test meals. ingestion of all experimental meals caused an increase in serum uric acid levels at 120 minutes an ... | 1992 | 1619189 |
| isoliquiritigenin, a strong nod gene- and glyceollin resistance-inducing flavonoid from soybean root exudate. | isoflavonoid signal molecules from soybean (glycine max (l.) merr.) seed and root exudate induce the transcription of nodulation (nod) genes in bradyrhizobium japonicum. in this study, a new compound with symbiotic activity was isolated from soybean root exudate. the isolated 2',4',4-trihydroxychalcone (isoliquiritigenin) is characterized by its strong inducing activity for the nod genes of b. japonicum. these genes are already induced at concentrations 1 order of magnitude below those required ... | 1992 | 1622242 |
| evaluation of substrates and storage conditions for preparing and maintaining starter cultures for tempeh fermentation. | heat-pasteurized cassava root, cowpeas, partially defatted peanuts, rice and soybeans were evaluated for their suitability to support growth and sporulation of the tempeh mold, rhizopus microsporus var. oligosporus, and the oncom mold, neurospora intermedia, at 25, 30 and 37 degrees c. the molds grew best and sporulated most luxuriantly on cassava and rice incubated at 37 degrees c. viability of molds remained high for up to 30 weeks when dried (a(w) 0.48), powdered substrates on which the molds ... | 1992 | 1622761 |
| effect of feeding finishing pigs extruded full-fat soybeans on performance and pork quality. | this experiment was designed to test whether changing the type and amount of added dietary fat would affect performance or meat quality. dietary fat was added as tallow or extruded full-fat soybeans (ffs). isoenergetic diets containing 10% ffs, 20% ffs, or 4% tallow were fed for 3 or 6 wk before slaughther. a conventional corn-soybean meal diet served as a no-added-fat control. each of the seven dietary groups contained 20 pigs, equally distributed among four pens, with barrows and gilts segrega ... | 1992 | 1644690 |
| characterization of lipid in loin and bacon from finishing pigs fed full-fat soybeans or tallow. | this experiment tested whether the lipid composition of pork could be altered by changes in dietary lipid, that what effects these changes would have on production and meat characteristics. pigs were fed diets containing 10 or 20%+ extruded full-fat soybeans (ffs) or 4% tallow for a 3- or 6-wk finishing period, with a corn-soybean meal diet fed as a control. seventy barrows and 70 gilts were allotted into seven treatment groups with four pens per group. analysis was on 28 experimental units, whi ... | 1992 | 1644691 |
| effects of ensiling on the digestibility and utilization of whole oilseeds by wethers. | two digestion and n metabolism trials were conducted with 35-kg wethers to assess the feeding value of whole cottonseed or soybeans ensiled with corn silage in 3.6-m3 concrete silos (1.8 x 1.4 m). dietary treatments for the cottonseed trial were ensiled whole cottonseed, untreated whole cottonseed, and 21 or 13% cottonseed meal in a basal diet of corn silage. there were no differences among treatments for dm and adf intakes. including ensiled or whole cottonseed in rations decreased digestibilit ... | 1991 | 1646241 |
| nolc, a rhizobium fredii gene involved in cultivar-specific nodulation of soybean, shares homology with a heat-shock gene. | rhizobium fredii strain usda257 does not nodulate soybean (glycine max (l.) merr.) cultivar mccall. mutant 257dh5, which contains a tn5 insert in the bacterial chromosome, forms nodules on this cultivar, but acetylene-reduction activity is absent. we have sequenced the region corresponding to the site of tn5 insertion in this mutant and find that it lies within a 1176bp open reading frame that we designate nolc. nolc encodes a protein of deduced molecular weight 43564. nucleotide sequences homol ... | 1991 | 1646377 |
| lectins from triticum vulgaris and limax flavus are universal antagonists of botulinum neurotoxin and tetanus toxin. | lectins from anguilla anguilla, artocarpus integrifolia, canavalia ensiformis, datora stramonium, glycine max, limax flavus, ricinus communis and triticum vulgaris were tested for their abilities to antagonize the binding of botulinum neurotoxin and tetanus toxin to rat brain membranes and to antagonize the ability of these toxins to block neuromuscular transmission in mouse phrenic nerve-hemidiaphragm preparations. lectins from limax flavus and triticum vulgaris, both of which have affinity for ... | 1991 | 1653841 |
| characterization of cytochromes c550 and c555 from bradyrhizobium japonicum: cloning, mutagenesis, and sequencing of the c555 gene (cycc). | the major soluble c-type cytochromes in cultured cells of bradyrhizobium japonicum usda 110 comprised a co-reactive c555 (mr, approximately 15,500) and a non-co-reactive c550 (mr, approximately 12,500). levels of cytochrome per gram of soluble protein in aerobic, anaerobic, and symbiotic cells were 32, 21, and 30 nmol, respectively, for c555 and 31, 44, and 65 nmol, respectively, for c550. the midpoint redox potentials (em,7) of the purified cytochromes were +236 mv for c555 and +277 mv for c550 ... | 1991 | 1660457 |
| influence of bovine somatotropin and nutrition on production and composition of milk from dairy cows. | forty-eight multiparous holstein cows were used in an experiment with a 2 x 2 factorial arrangement of treatments to study interactions of bst and nutrient density during wk 5 through 20 postpartum. main effects were bst (0 or 10.3 mg/d) and diet (control or high protein and energy). the control diet was formulated to contain 16.3% cp and 1.65 mcal of nel/kg of dm; the high protein and energy diet was formulated to contain 18% cp and 1.74 mcal of nel/kg of dm with additional energy as fat from e ... | 1991 | 1661753 |
| human vntr sequences in porcine htf-islands. | mapping of complex genomes has been influenced substantially by the isolation of locus-specific, but repetitive dna elements known as vntrs. since a high gc-content is characteristic of most of these elements one might expect them to be clustered at least partially in cpg-islands. to address this question we have constructed a porcine liver dna noti-linkage library in puc18 using isolated htf-islands. hpaii tiny fragments ranging from less than 100 bp to 1 kb in length were randomly selected and ... | 1991 | 1678358 |
| [determination of lectin receptors on the surface membranes of lymphoid cells]. | the authors describe a method for the detection of carbohydrate determinants contained by the cellular surface membrane glycosylated biopolymers, based on the use of horse radish peroxidase-conjugated arachis, helix pomatia, ricinus communis, lens, glycine max, sophora japonica lectins. the pattern of lectin receptor distribution on normal human blood lymphocytes, tonsillar lymphoid cells, and in some forms of leukemia and lymphoma is shown. the authors consider it essential that a lectin kit be ... | 1990 | 1692358 |
| glycoconjugate expression of cells of human anagen hair follicles during keratinization. | changes in the expression of glycoconjugates in cells of the inner root sheath (irs) and outer root sheath (ors) of human anagen hair follicles were investigated by lectin histochemistry. concanavalin a (con a) and ricinus communis (rca-i) stained hair follicle cells regardless of their differentiation stages. in irs, ulex europeaus-i (uea-i) bound to the surface of the cells as soon as they were morphologically defined, and glycine max (sba) stained as their differentiation progressed. innermos ... | 1990 | 1700646 |
| lectin histochemical characteristics of the canine female mammary gland. | twelve biotinylated lectins and an avidin-biotin-peroxidase method were used to detect and localize specific carbohydrate residues on formalin-fixed, paraffin-embedded female canine mammary gland sections. histologic sections from 3 lactating and 7 nonlactating mixed-breed dogs (age 5.6 +/- 0.35 years) were incubated with arachis hypogea agglutinin (peanut agglutinin; pna), concanavalia ensiformis agglutinin (cona), dolichos biflorus agglutinin (dba), glycine max agglutinin (sba), griffonia simp ... | 1990 | 1700652 |
| the transposable element tam1 from antirrhinum majus shows structural homology to the maize transposon en/spm and has no sequence specificity of insertion. | we present the genomic structure of tam1, a transposable element from antirrhinum majus. the tam1 element is 15.2 kb long and includes two genes that are transcribed to produce a 2.4 kb (tnp1) and a 5 kb mrna (tnp2). these transcripts partially overlap and the exons are scattered over the whole element. tnp1 encodes a 53 kda protein as deduced from the cdna sequence. the 5 kb transcript of tnp2 contains an open reading frame that shares 45% homology with part of the tnpd gene of en/spm from maiz ... | 1991 | 1715971 |
| elisa analysis of soybean trypsin inhibitors in processed foods. | soybean proteins are widely used in human foods in a variety of forms, including infant formulas, flour, protein concentrates, protein isolates, soy sauces, textured soy fibers, and tofu. the presence of inhibitors of digestive enzymes in soy proteins impairs the nutritional quality and possibly the safety of soybeans and other legumes. processing, based on the use of heat or fractionation of protein isolates, does not completely inactivate or remove these inhibitors, so that residual amounts of ... | 1991 | 1716818 |
| determining optimal heat treatment of soybeans by measuring available lysine chemically and biologically with rats to maximize protein utilization by ruminants. | soybeans were heated in a forced air oven at 120 and 130 degrees c for 60 and 180 min, and at 140, 150 and 160 degrees c for 10, 30, 60, 90 and 120 min. two types of measurements were used to determine optimal heat treatment of soybeans to maximize protein utilization by ruminants. one was to estimate the rate and extent of protein degradation in the rumen using an in vitro ruminal system. the second was to determine the nutritional availability of lysine. methods used to determine available lys ... | 1992 | 1729465 |
| lectin binding to gp60 decreases specific albumin binding and transport in pulmonary artery endothelial monolayers. | the effect of albumin binding to cultured bovine pulmonary artery endothelial cell (bpaec) monolayers on the transendothelial flux of 125i-labelled bovine serum albumin (bsa) was examined to determine its possible role on albumin transcytosis. the transport of 125i-bsa tracer across bpaec grown on gelatin- and fibronectin-coated filters (0.8 microns pore diam.) was affected by the presence of unlabelled bsa in the medium in that transendothelial 125i-bsa permeability decreased, reaching a 40% re ... | 1991 | 1744181 |
| identification of lectin binding proteins in human tears. | the identity of glycoproteins in stimulated normal human tears was investigated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of tears onto minigels, blotting, and subsequent incubation with different biotinylated lectins (concanavalin a [con a], peanut agglutinin [pna], glycine max agglutinin [sba], phaseolus vulgaris agglutinin, wheat germ agglutinin [wga, native form], artocarpus integrifolia agglutinin [jacalin], and pisum sativum agglutinin). control proteins inclu ... | 1991 | 1748557 |
| effects of heating time and sodium metabisulfite on the nutritional value of full-fat soybeans for chicks. | two 21-d trials were conducted to evaluate the effect of heating time and sodium metabisulfite (smbs) on the nutritional value of full-fat soybeans for chicks. in trial 1, four pen-replicates of eight chicks each were fed corn-based diets (19% cp; 3,167 kcal of me/kg) containing either 44% cp soybean meal or full-fat soybeans. the soybeans either were unheated or were autoclaved at 121 degrees c for 10, 20, 30, 40, 60, or 90 min. soybean oil was added to the soybean meal diet to make it isoenerg ... | 1991 | 1752823 |
| changes in lectin-binding patterns during late fetal development of the rat colon. | lectin-binding and histochemical studies were integrated with a morphological description of colon development in rat fetuses to determine whether changes in glycoprotein sugars could be identified with stages of colon organogenesis. at 16 days gestation the colon consisted of a minute lumen surrounded by 3-5 layers of cuboidal, stratified epithelium, a basement membrane and undifferentiated mesenchyme. as development proceeded, epithelial spaces eventually fused with the main lumen, and fingers ... | 1991 | 1755286 |
| lactational response of dairy cows to increased dietary crude protein with added fat. | thirty-six holstein cows were assigned to one of three diets containing soybean meal (16% cp), added fat from extruded soybeans (16% cp), and added fat and protein from extruded soybeans plus soybean meal (18% cp) to determine whether feeding additional protein would prevent the depression in milk protein percentages usually experienced when cows are fed additional fat. total mixed diets containing 25% corn silage, 25% alfalfa hay, and 50% of respective concentrate mixtures were fed individually ... | 1991 | 1757629 |
| evolution of enzymatic regulation of prostaglandin action: novel connections to regulation of human sex and adrenal function, antibiotic synthesis and nitrogen fixation. | the recent determination of the amino acid sequences of enzymes that metabolize prostaglandins and steroids has revealed interesting connections between some of these enzymes. human placental 15-hydroxyprostaglandin dehydrogenase, which catalyzes the oxidation of the c15 alcohol on prostaglandins e2 and f2 alpha, is homologous to 11 beta-hydroxysteroid, 17 beta-hydroxysteroid, and 3 alpha, 20 beta-hydroxysteroid dehydrogenases. that is, these four enzymes are derived from a common ancestor. more ... | 1991 | 1763199 |
| enhancement of soybean nodulation by bacillus cereus uw85 in the field and in a growth chamber. | seed treatments with bacillus cereus uw85 increased nodulation of soybeans in three field seasons and in three different sterilized soils in the growth chamber. in the field, 28 and 35 days after planting, uw85-treated plants had 31 to 133% more nodules than untreated plants. from 49 days after planting until seed harvest, there were no significant differences between nodulation of uw85-treated plants and untreated control plants. in the growth chamber, in sterilized soil-vermiculite mixtures, a ... | 1991 | 1768151 |
| production & formulation of bacillus thuringiensis var. israelensis & b. sphaericus 1593. | three fermentation media each for bulk growth of b. thuringiensis var. israelensis and b. sphaericus 1593 were formulated using defatted groundnut cake (arachis hypogea) as the first nitrogen source and gram flour (cicer arientinum), soy bean (glycine max) and defatted milk powder as the second nitrogen source. medium containing gram flour showed highest toxicity (14.45 micrograms/l) in case of b. thuringiensis var. israelensis whereas medium containing milk powder was found to be highly toxic w ... | 1991 | 1778620 |
| feeding heat-treated full fat soybeans to cows in early lactation. | forty-six multiparous holstein cows were fed one of three total mixed diets from 15 to 119 d postpartum with alfalfa silage as the only forage. each diet contained 50% forage and 50% concentrate on a dm basis. diets were formulated to be isonitrogenous by replacing corn and solvent soybean meal with raw soybeans or heat-treated soybeans. the proportion of protein supplement in the diet on a dm basis was 10% soybean meal, 13% raw soybeans, or 13% heat-treated soybeans. the soybeans were heat-trea ... | 1991 | 1779058 |
| morphological study of lectin binding in the rabbit temporomandibular joint disc. | glycoconjugates of the extracellular matrix are important for the normal mechanical functions of connective tissue structures such as the temporomandibular joint disc. since lectins are known to bind to sugar residues with high affinity, a variety of lectins were used to study the presence and distribution of glycoconjugates in the temporomandibular joint disc. discs were removed from 6 to 8-month-old rabbits and either sectioned in a cryostat and processed for light microscopy or fixed in 2% gl ... | 1991 | 1783561 |
| [soy and corn flour precooked with microwaves and its use in the preparation of arepas]. | unhusked corn and soy grits were used as raw material, with a particle size ranging between 10 and 20 mesh (astm). the results obtained in this study reveal that microwave heating is effective in destroying the antinutritional factors present in soybeans. the trypsin inhibitor activity, in effect, was reduced to a 76% inactivation. the hemagglutinating titer was labile to the heating process, showing values of +8 to +3 for the full-fat soy flour and precooked soy flour, respectively. the quality ... | 1991 | 1824518 |
| lectin-binding patterns in the spermatogenic cells of the shiba goat testis. | lectin-binding patterns in the testis of the sexually mature goat were investigated by light and transmission electron microscopy. dolichos biflorus agglutinin (dba) and griffonia simplicifolia agglutinin-i (gs-i) were negative in the seminiferous epithelium, but soybean (glycine max) agglutinin (sba), griffonia simplicifolia agglutinin-ii (gs-ii) and peanut (arachis hypogaea) agglutinin (pna) were positive in the acrosomal vesicle of the golgi-phase spermatids and in the acrosome of the cap-, a ... | 1991 | 1836378 |
| induced plant responses to pathogen attack. analysis and heterologous expression of the key enzyme in the biosynthesis of phytoalexins in soybean (glycine max l. merr. cv. harosoy 63). | in soybean (glycine max l.), pathogen attack induces the formation of glyceollin-type phytoalexins. the biosynthetic key enzyme is a reductase which synthesizes 4,2', 4'-trihydroxychalcone in co-action with chalcone synthase. screening of a soybean cdna library from elicitor-induced rna in lambda gt11 yielded two classes of reductase-specific clones. the deduced proteins match to 100% and 95%, respectively, with 229 amino acids sequenced in the purified plant protein. four clones of class a were ... | 1991 | 1840523 |
| molecular cloning and expression in photosynthetic bacteria of a soybean cdna coding for phytoene desaturase, an enzyme of the carotenoid biosynthesis pathway. | carotenoids are orange, yellow, or red photo-protective pigments present in all plastids. the first carotenoid of the pathway is phytoene, a colorless compound that is converted into colored carotenoids through a series of desaturation reactions. genes coding for carotenoid desaturases have been cloned from microbes but not from plants. we report the cloning of a cdna for pds1, a soybean (glycine max) gene that, based on a complementation assay using the photosynthetic bacterium rhodobacter caps ... | 1991 | 1862081 |
| nutritional evaluation of soybeans varying in trypsin inhibitor content. | a series of experiments was conducted to assess, by various methods, the nutritional value of a raw, kunitz inhibitor-free, low trypsin-inhibitor soybean variant (lts) in comparison with raw conventional soybeans (rcs) and heated dehulled soybean meal (hds). the gross energy, protein, and amino acid concentrations of lts were similar to those of rcs. the protein quality of the soybeans was compared in two trials in which young chicks were fed 9% or 16% cp diets containing one of the soybeans as ... | 1991 | 1876565 |
| monitoring by epifluorescence microscopy of organelle dna fate during pollen development in five angiosperm species. | the fates of mitochondrial and plastid nucleoids during pollen development in six angiosperm species (antirrhinum majus, glycine max, medicago sativa, nicotiana tabacum, pisum sativum, and trifolium pratense) were examined using epifluorescence microscopy after double staining with 4',6-diamidino-2- phenylindole (dapi) to stain dna and with a potentiometric dye (either dioc7 or rhodamine 123) for visualization of metabolically active mitochondria. from the pollen mother cell stage to the microsp ... | 1991 | 1879613 |
| chemical control of interstrain competition for soybean nodulation by bradyrhizobium japonicum. | previous research has shown that a significant limitation to the agricultural use of improved rhizobial inoculant strains is competition from the indigenous soil population. in this work, we sought to test whether chemical inhibitors of flavonoid-induced nod gene expression in bradyrhizobium japonicum could be identified and utilized to affect interstrain competition for nodulation of soybeans. approximately 1,000 structural and functional analogs of the known, natural inducers of nod gene expre ... | 1991 | 1892378 |
| effect of heat on the nutritional quality and safety of soybean cultivars. | to evaluate whether soybean strains with reduced levels of trypsin inhibitors have enhanced nutritional and safety characteristics, we measured protease inhibitor content of a standard cultivar (williams 82) and an isoline (l81-4590) lacking the kunitz trypsin inhibitor, using enzyme inhibition assays and enzyme-linked immunosorbent assays (elisa). less heat was needed to inactivate the remaining trypsin inhibitory activity of the isoline than that of the standard soybean cultivar. in fact, auto ... | 1991 | 1897401 |
| lipoxygenases from soybeans and rabbit reticulocytes: inactivation and iron release. | various inactivation methods were applied to lipoxygenases from soybean (isoenzyme 1) and rabbit reticulocytes to compare the inactivation behaviour of both enzymes and to elucidate the state of the iron which is known to be involved in the catalytic reaction of lipoxygenases: 1. titration of the enzyme with mercury compounds shows that there are one or two sh groups responsible for the loss of activity in the presence of mercury. the sh groups seem not to be involved in the tight iron binding. ... | 1991 | 1908675 |
| the response of lactating dairy cows to increasing levels of whole roasted soybeans. | twenty-four holstein cows, including four primiparous and four ruminally cannulated, were in replicated 4 x 4 latin squares with 21-d periods to determine the effects of feeding level of whole roasted soybeans on lactation performance and rumen function. cows were fed rations containing a 50:50 forage:concentrate ratio with 0, 12, 18, or 24% of diet dm as whole roasted soybeans. rations contained 16.8, 16.9, 18.6, and 19.7% cp and 1.68, 1.71, 1.72, and 1.74 mcal nel/kg dm, respectively. milk pro ... | 1991 | 1918534 |
| total lactational response to added fat during early lactation. | the theory that feeding cows additional fat during early lactation will result in greater total lactational production and improved reproductive efficiency was evaluated using 305-d lactational data from 94 cows fed control diets and 96 cows fed additional dietary fat during wk 4 through 16 postpartum. added fat in diets was supplied by oilseeds, either soybeans or sunflowers, in previously reported studies. milk yield during wk 4 through 16 postpartum was 2.8% higher for cows fed added fat. tot ... | 1991 | 1918536 |
| research note: effect of soybeans varying in trypsin inhibitor content on performance of laying hens. | a 6-wk experiment was conducted with commercial laying hens (35 wk of age) to study the effects of feeding raw, conventional, full-fat soybeans (csb) and raw, kunitz trypsin inhibitor-free, full-fat soybeans (kfsb) on layer performance. dietary treatments consisted of corn and soybean meal diets formulated to contain 16% total protein with 100, 72, or 48% of the soybean protein from csb or kfsb. the remainder of the dietary soybean protein was provided as dehulled soybean meal (dsbm). egg produc ... | 1991 | 1956863 |
| the bradyrhizobium japonicum nola gene and its involvement in the genotype-specific nodulation of soybeans. | several soybean genotypes have been identified which specifically exclude nodulation by members of bradyrhizobium japonicum serocluster 123. we have identified and sequenced a dna region from b. japonicum strain usda 110 which is involved in genotype-specific nodulation of soybeans. this 2.3-kilobase region, cloned in pmjs12, allows b. japonicum serocluster 123 isolates to form nodules on plants of serogroup 123-restricting genotypes. the nodules, however, were ineffective for symbiotic nitrogen ... | 1991 | 1988958 |
| bradyrhizobium japonicum has two differentially regulated, functional homologs of the sigma 54 gene (rpon). | recognition of -24/-12-type promoters by rna polymerase requires a special sigma factor, sigma 54 (rpon ntra glnf). in the nitrogen-fixing soybean symbiont bradyrhizobium japonicum, two functional, highly conserved rpon genes (rpon1 and rpon2) were identified and sequenced. the two predicted b. japonicum rpon protein sequences were 87% identical, and both showed different levels of homology to the rpon proteins of other bacteria. downstream of rpon2 (but not of rpon1), two additional open readin ... | 1991 | 1991712 |
| raw soy and purified proteinase inhibitors induce the appearance of inhibitor-resistant trypsin and chymotrypsin activities in wistar rat duodenal juice. | rats were fed raw soybeans or purified soybean proteinase inhibitors by tube to see whether they were able to produce inhibitor-resistant trypsin, as previously demonstrated in humans. their duodenal chyme contained only 20-50% of the enzymatic activities of animals fed bovine serum albumin (bsa) as test protein. however, both tryptic and chymotryptic activities had considerable resistance to low- and high-molecular-weight inhibitors of serine proteinases. in particular, the tryptic activity dem ... | 1991 | 2007905 |
| identification of glya as a symbiotically essential gene in bradyrhizobium japonicum. | a bradyrhizobium japonicum tn5 mutant (strain 3160) induced numerous, tiny, white nodules which were dispersed over the whole root system of its natural host plant, soybean (glycine max). these ineffective, nitrogen non-fixing pseudonodules were disturbed at a very early step of bacteroid and nodule development. subsequent cloning and sequencing of the dna region mutated in strain 3160 revealed that the tn5 insertion mapped in a gene that had 60% homology to the escherichia coli glya gene coding ... | 1991 | 2014004 |
| value of raw soybeans and soybean oil supplementation in sow gestation and lactation diets: a cooperative study. | a cooperative study using 215 sows during two parities (349 litters) was conducted at six stations to determine the effect of raw soybeans in gestation and lactation diets on sow and litter performance. sows were bred and allotted to fortified corn diets containing either soybean meal (control) or raw soybeans. a corn-soybean meal-soybean oil diet, isocaloric to the raw soybean diet, was included as a third treatment at three stations. all diets contained 14% cp. these diets were fed during both ... | 1991 | 2016195 |
| [a study of the inheritance and linkage of resistance to four strains of soybean mosaic virus in soybeans]. | ten susceptible x resistant and resistant x resistant crosses were made among eight varieties, of which seven were previously identified as resistant to one to four smv strains, in order to investigate the inheritance of resistance to the four strains (sa, sc, sg, and sh) and the linkage relationship among the resistant genes. seeds from f2 individual plants were randomly divided and planted in two separated nurseries, in which the seedlings were inoculated with two different strains. the genoty ... | 1991 | 2029427 |
| histochemical study on the bile duct system of normal mongolian gerbils. | the bile duct system of normal mongolian gerbils was examined histochemically. the luminal surface membrane and apical cytoplasm of the biliary and gallbladder epithelial cells were stained with periodic acid-schiff (pas), alcian blue, ph 2.5 (ab) and high iron diamine (hid)-ab, and many epithelial cells of the common bile duct and gallbladder had weakly pas-positive granular material in their supranuclear cytoplasm. lectin-histochemically, these cells had binding sites to concanavalia ensiformi ... | 1991 | 2044662 |
| the effect of a change in dietary habit upon maze learning ability in rats. | an experimental psychological study was undertaken to assess the effect of a change in dietary habit upon behavioural development, using as a laboratory animal male wistar rats maintained on natural foods and a processed food. forty male wistar rats were weaned at 21 days of age and divided into two equal groups. group 1 was fed natural food (pressed wheat, dried fish, peanuts, cabbage and soybeans) and group 2 was fed powdered food. at 68 days of age, the rats were tested in a t-maze. the resul ... | 1991 | 2051250 |
| [isolation and characteristics of insulin-binding proteins from soybeans]. | two soybean insulin-binding proteins were isolated using affinity chromatography on insulin-sepharose. both proteins have molecular mass about 39 kda and consist of two subunits linked by disulphide bonds. according to the amino acid composition and n-terminal sequences of the subunits, these proteins, characterized by the absence of free thiol groups and sugar residues, are variants of the previously described soybean basic 7s globulin. the blotted proteins as well as their subunits were shown ... | 1991 | 2064630 |
| sphingolipids and glycerolipids. i. chemical structures and ionophoretic activities of soya-cerebrosides i and ii from soybean. | two glycosphingolipids named soya-cerebrosides i and ii were isolated from soybean, the seeds of glycine max merrill (leguminosae), and their chemical structures have been elucidated on the basis of physicochemical evidence and several chemical degradation reactions. by using a newly constructed liquid membrane-type apparatus (w-08) for measurement of ion-transport and ion-binding activities and by employing a method using human erythrocyte membranes for measurement of ion-permeability, it has b ... | 1990 | 2085876 |
| crystallization and preliminary x-ray analysis of soybean lipoxygenase-1, a non-heme iron-containing dioxygenase. | crystals of lipoxygenase-1 from soybeans have been grown by the method of vapor diffusion in the presence of sodium formate, ammonium acetate, and lithium chloride at ph 7.0. this enzyme contains a non-heme iron and is closely related to a human lipoxygenase found in leukocytes that participates in the biosynthesis of leukotrienes and lipoxins. the crystals are monoclinic space group c2 with cell dimensions of a = 183.8 a, b = 123.2 a, c = 94.3 a and beta = 102.9 degrees. they diffract beyond 2. ... | 1990 | 2115880 |
| lectins and heparin-binding features of human spermatozoa as analyzed by flow cytometry. | sperm cell surface characteristics may serve as a useful diagnostic feature for the evaluation of seminal samples, but to date no useful analytical method(s) is available for their routine quantitative analysis. fluorescein isothiocyanate-conjugated lectins, proteins with unique and specific saccharide-binding characteristics, and heparin are potentially useful markers for such an analytical procedure. a protocol is outlined whereby frozen semen is thawed and treated with either fluorescein isot ... | 1990 | 2124086 |
| performance and plasma amino acids of growing calves fed corn silage supplemented with ground soybeans, fishmeal and rumen-protected lysine. | a 100-d growth study was conducted to evaluate performance and plasma amino acid (aa) responses of 96 crossbred beef calves (220 kg) with ad libitum access to corn silage and supplemented with ground soybeans (gsb) with or without added fishmeal (fm) and (or) rumen-protected lysine (lys). calves were allotted by breed, sex and weight to four treatments with three replicate pens of eight calves per pen. the treatments were: gsb, gsb + lys, gsb + fm and gsb + fm + lys. the isonitrogenous supplemen ... | 1990 | 2124581 |
| histochemical study on the bile duct system of normal rats. | the bile duct system of normal rats was examined histochemically. although the apical surface of biliary and glandular epithelial cells was positively stained with concanavalia ensiformis (con a), dolichos biflorus (dba), glycine max (sba), ulex europeus-i (uea-i) and triticumvulgaris (wga), the cytoplasm did not apparently react with any lectins. therefore these cells might not play an important role in an active secretion of mucin. on the other hand, the cytoplasm of goblet cells was positivel ... | 1990 | 2144826 |
| influence of media composition on the production of delta-endotoxin by bacillus thuringiensis var. thuringiensis. | powders of edible leguminous seeds, greengram (vigna radiata) or soybean (glycine max), were used as the major protein source with different combinations of soluble starch and/or cane sugar molasses as the major carbohydrate source for the production of delta-endotoxin by bacillus thuringiensis var. thuringiensis serotype 1 in submerged fermentation. the primary product (lyophilized with 6 g of lactose) yield was 8.7 to 9.1 g/liter from media with dehusked greengram powder and 9.7 to 10.3 g/lite ... | 1990 | 2156939 |
| lack of fructose-1,6-bisphosphatase in a range of higher plants that store starch. | the aim of this work was to discover whether fructose-1,6-bisphosphatase (fbpase) is present in higher-plant cells that synthesize storage starch. the following were examined: suspension cultures of soybean (glycine max), tubers of potato (solanum tuberosum), florets of cauliflower (brassica oleracea), developing endosperm of maize and of sweet corn (zea mays), roots of pea (pisum sativum), and the developing embryos of round and wrinkled varieties of pea. unfractionated extracts of each tissue ... | 1990 | 2173563 |
| association of bacteria with the fungal fermentation of soybean tempe. | bacteria grew to viable populations of 10(8)-10(9) cfu/g during the fermentation of soybeans into tempe with the fungus, rhizopus oligosporus. bacillus pumilus and b. brevis were the predominant bacterial species, reaching populations of approximately 10(8) cfu/g during the 48 h fermentation. species of streptococcus faecium, lactobacillus casei, klebsiella pneumoniae and enterobacter cloacae also contributed to the fermentation and achieved populations of 10(6)-10(7) cfu/g. | 1990 | 2179196 |
| ammoniation of straw by urea: influence of addition of soybeans and/or molasses on characteristics of treatment. | | 1990 | 2206314 |
| evaluation of various extracted vegetable oils, roasted soybeans, medium-chain triglyceride and an animal-vegetable fat blend for postweaning swine. | a total of 280 crossbred pigs weaned at 21 d of age and weighing approximately 6 kg were utilized in five replicates to evaluate pig growth responses when fed a basal diet or one of several dietary lipid sources during a 4-wk postweaning period. a basal corn-soybean meal-corn starch-dried whey diet was compared with diets supplemented at a 7.75% level with one of the following lipid sources: corn oil, coconut oil, soybean oil, medium-chain triglyceride (mct) or an animal-vegetable blend. a sixth ... | 1990 | 2211404 |
| evaluation of various nitrogen supplements in starter diets for growing holstein steers and their effects on ruminal bacterial fermentation in continuous culture. | concurrent in vivo and in vitro studies were conducted to evaluate urea (u), soybean meal (sbm), ground soybeans (raw), extruded soybeans (es) or extruded soybeans plus urea (es + u) as primary supplemental n sources in starter diets for holstein steers. three groups of 48 holstein steers each were fed five different starter diets to 181 kg bw in three experimental periods over 2 yr. average daily gains were similar (p greater than .05) for steers fed es + u (1.12 kg), es (1.08 kg) and sbm (1.09 ... | 1990 | 2211424 |
| carbohydrate binding activities of bradyrhizobium japonicum. i. saccharide-specific inhibition of homotypic and heterotypic adhesion. | bradyrhizobium japonicum (r110d) exhibited four saccharide-specific binding activities: (a) adsorption to sepharose beads containing covalently coupled lactose; (b) homotypic agglutination through one pole of the cell (star formation); (c) heterotypic adhesion to the cultured soybean cell line, sb-1; and (d) attachment to roots of soybean plants. each of these binding activities can be inhibited by the addition of galactose or lactose, but not by derivatives such as n-acetyl-d-galactosamine or m ... | 1990 | 2211829 |
| soybeans inhibit mammary tumors in models of breast cancer. | | 1990 | 2217394 |
| effects of feeds on flavor of red meat: a review. | the effects of diet ingredients on red meat flavor are dependent on the type of diet, and, to a large extent, on the species: pork, mutton or lamb and beef. sensory analysis of meat flavor has been used in most of the studies on the effects of feeds on meat flavor. in general, high-energy grain diets produced a more acceptable or a more intense flavor in red meats than low-energy forage or grass diets. feeding pigs unsaturated fats increases the unsaturation in pork fat but results in only minor ... | 1990 | 2286578 |
| characterization of a proline-rich cell wall protein gene family of soybean. a comparative analysis. | further characterization of a proline-rich cell wall protein gene family from soybean (glycine max (l.) merr) has been accomplished by the isolation and sequence analysis of two additional genes, sbprp2 and sbprp3, which encode mrnas of 1050 and 650 nucleotides in length, respectively. like the proline-rich protein gene, sbprp1, which was previously reported (hong, j. c., nagao, r. t., and key, j. l. (1987) j. biol. chem. 262, 8367-8376), these two sbprp genes encode proteins having a signal pep ... | 1990 | 2303411 |
| secretory glycoconjugates of a mucin-synthesizing human colonic adenocarcinoma cell line. analysis using double labeling with lectins. | lectins were used to characterize mucin glycoproteins and other secretory glycoconjugates synthesized by a human colon adenocarcinoma-derived cell line which expresses a goblet cell phenotype. despite being clonally derived, ht29-18n2 (n2) cells, like normal goblet cells in situ were heterogeneous in their glycosylation of mucin. only wheat-germ agglutinin, which recognizes n-acetylglucosamine and sialic acid residues, and succinylated wheatgerm agglutinin, which binds n-acetylglucosamine, stain ... | 1990 | 2312359 |
| selenium content of foods consumed in otago, new zealand. | selenium (se) concentrations were determined in over 600 foods sampled in dunedin, new zealand. foods included those produced in the region, foods which were expected to contribute significantly to the total se intake, breads and other wheat products which might be affected by the importation of australian wheat, and imported vegetarian foods. foods with the highest se concentrations were brazil nuts, sunflower seeds and all varieties of fish and kidneys. somewhat lower concentrations were found ... | 1990 | 2320341 |
| proposed regulatory pathway encoded by the nodv and nodw genes, determinants of host specificity in bradyrhizobium japonicum. | bradyrhizobium japonicum is the root nodule endosymbiont of soybean (glycine max), mung bean (vigna radiata), cowpea (vigna unguiculata), and siratro (macroptilium atropurpureum). we report the characteristics of a nodulation-gene region of b. japonicum that contributes only marginally to the bacterium's ability to nodulate soybean but is essential for the nodulation of the three alternative hosts. this dna region consists of two open reading frames designated nodv and nodw. the predicted amino ... | 1990 | 2320582 |
| purification and characterization of a novel calcium-dependent protein kinase from soybean. | a novel calcium-dependent protein kinase (cdpk) previously reported to be activated by the direct binding of ca2+, and requiring neither calmodulin nor phospholipids for activity [harmon, a.c., putnam-evans, c.l., & cormier, m.j. (1987) plant physiol. 83, 830-837], was purified to greater than 95% homogeneity from suspension-cultured soybean cells (glycine max, l. wayne). purification was achieved by chromatography on deae-cellulose, phenyl-sepharose, sephadex g-100, and blue sepharose. the puri ... | 1990 | 2334677 |
| [the evaluation of the genotoxic effects of samples of industrial and domestic sewage. ii. the mutagenic and recombinant effects of sewage in the soybean (glycine max. (l.)) test system]. | the genotoxicity of the industrial and domestic sewage has been studied on the soybean test-system glycine max (l.). it makes it possible to take into account different types of genetic disturbances according to appearance of various spot types on sprout leaves (somatic mosaicism). | 1990 | 2346766 |
| nodule formation in soybeans by exopolysaccharide mutants of rhizobium fredii usda 191. | production of exopolysaccharides by rhizobium has been linked with efficient invasion and nodulation of leguminous plant roots by the bacteria. exopolysaccharide-deficient (exo) mutants of rhizobium fredii usda 191 were isolated following tn5-insertion mutagenesis. five phenotypically unique exo mutants were investigated for exopolysaccharide synthesis and their ability to nodulate soybeans. the exopolysaccharides produced by these mutants were analysed for polysaccharide composition by column c ... | 1990 | 2351951 |
| [interpretation of thermograms of protein denaturation in non-equilibrium conditions]. | data are presented concerning the effect of heating rate on the denaturation parameters of small and oligomeric globular proteins: kunitz trypsin inhibitor from soybeans and 1,5-ribulose bisphosphate carboxylase from tobacco leaves. substantional dependence of denaturation temperature on the heating rate reflects non-equilibrium pattern of denaturation of these proteins under experimental conditions. to interpret these data a kinetic approach is proposed, which permits determination of equilibri ... | 1990 | 2369595 |
| differences in expression of oligosaccharide determinants by phenotypically distinct sublines of the dunning 3327 rat prostate cancer. | oligosaccharides expressed by the 3327-h and 3327-mat lylu sublines of the dunning rat prostate cancer model have been compared in formalin-fixed and routinely paraffin-embedded tumour tissues. binding by lectins of defined specificity has been employed to identify expression of seven oligosaccharide structures by primary and metastatic prostatic carcinoma cells. neuraminidase digestion was employed to reveal determinants masked by sialic acid. the presence of core man alpha 1----3(man alpha 1-- ... | 1990 | 2388349 |
| production of enterotoxin a and thermonuclease by staphylococcus aureus in legumes. | growth, enterotoxin a (sea) and thermonuclease (tnase) production of s. aureus (strains cp 7 and fri 722) was determined in media produced from the following heat or irradiation sterilized legumes: peas, black beans, mung beans, adzuki beans and soybeans. media containing the five legumes alone or in combination with brain heart infusion broth (bhi) were tested. with the exception of heat-sterilized black beans and adzuki beans, s. aureus growth was excellent in all media with cell counts after ... | 1990 | 2397154 |
| improved procedure for the detection of activity of lipoxygenases on electropherograms. | several procedures for activity staining of lipoxygenases were compared. the best results were obtained with the o-dianisidine method of de lumen and kazeniac which had to be modified, however, with respect to the conditions of electrophoretic separation, treatment of the gel after separation, incubation conditions and staining solution, to achieve satisfactory detection of the lipoxygenases from wheat grains, pea seeds, soybeans and rabbit reticulocytes. | 1985 | 2417596 |
| evidence for glycoconjugate in nociceptive primary sensory neurons and its origin from the golgi complex. | glycoconjugates with terminal galactose residues were localized in rat spinal cord and spinal ganglia using lectin-hrp conjugates of griffonia simplicifolia and glycine max agglutinins. alternate staining of serial sections with hrp-labelled lectins and an antibody for substance p (sp) showed staining in identical primary sensory neurons with both methods. similarly, lectin-reactive as well as sp-positive fibers were found in rexed laminae i and ii, lissauer's tract, the spinal nucleus and tract ... | 1986 | 2425897 |
| functional analysis of regulatory elements in a plant embryo-specific gene. | previously we demonstrated the expression of a plant embryo-specific gene encoding the alpha' subunit of beta-conglycinin, a seed storage protein of soybean (glycine max), in transgenic petunia plants. to examine the regulatory elements that control the expression of this embryo-specific gene (gmg17.1), a series of deletion mutants was made that contain the alpha'-subunit gene flanked in the 5' direction from +14 nucleotides to -8.5 kilobases (kb) relative to the site of transcription initiation ... | 1986 | 2430294 |
| effect of a trypsin inhibitor from soybeans (bowman-birk) on the secretory activity of the human pancreas. | this study was undertaken to clarify the issue of whether feedback regulation of pancreatic enzyme secretion occurs in humans. a naturally occurring inhibitor of trypsin and chymotrypsin, the bowman-birk inhibitor of soybeans, was used to reduce the activities of these enzymes normally secreted by the pancreas into the duodenum. pure pancreatic juice was collected by endoscopic retrograde cannulation of the pancreatic duct in a protocol consisting of three periods: period 1 (15 min), collections ... | 1988 | 2446949 |
| two host-inducible genes of rhizobium fredii and characterization of the inducing compound. | random transcription fusions with mu d1(kan lac) generated three mutants in rhizobium fredii (strain usda 201) which showed induction of beta-galactosidase when grown in root exudate of the host plants glycine max, phaseolus vulgaris, and vigna ungliculata. two genes were isolated from a library of total plasmid dna of one of the mutants, 3f1. these genes, present in tandem on a 4.2-kilobase hindiii fragment, appear in one copy each on the symbiotic plasmid and do not hybridize to the rhizobium ... | 1988 | 2447061 |
| high and low inhibitor soybean meals affect human duodenal proteinase activity differently: in vivo comparison with bovine serum albumin. | the objective of this study was to investigate the effects of high and low inhibitor soybean meals on the duodenal enzyme activities and on the possible regulatory role of gastrointestinal hormones in the pancreatic response. after an overnight fast, 11 healthy volunteers received an intraduodenal infusion of saline for 60 min. this was followed by infusion of either of three test meals: extract of raw soybeans (rs), a low inhibitor soy protein isolate (spi) or bovine serum albumin (bsa), 10 g/h ... | 1988 | 2451718 |
| glycoconjugates in normal human kidney. a histochemical study using 13 biotinylated lectins. | the avidin-biotin-peroxidase complex technique was used with 13 lectins to study the glycoconjugates of normal human renal tissue. the evaluated lectins included triticum vulgaris (wga), concanavalin ensiformis (cona), phaseolus vulgaris leukoagglutinin and erythroagglutinin (pha-l and pha-e), lens culinaris (lca), pisum sativum (psa), dolichos biflorus (dba), glycine max (sba), arachis hypogaea (pna), sophora japonica (sja), bandeiraea simplicifolia i (bsl-i), ulex europaeus i (uea-i) and ricin ... | 1988 | 2466021 |
| o-antigen from bradyrhizobium japonicum lipopolysaccharide inhibits intercellular (symplast) communication between soybean (glycine max) cells. | the technique of fluorescence redistribution after photobleaching was utilized to measure intercellular movement of low molecular weight fluorescent hydrophilic substances across the cell wall/membrane interface between contiguous soybean (glycine max (l.) merr. cv. mandarin) root cells (sb-1 cell line) in tissue culture. lipopolysaccharide (lps) purified from bradyrhizobium japonicum r110d, a gram-negative bacterium that normally infects and induces nodulation in soybean roots in vivo, inhibits ... | 1989 | 2473070 |
| differential regulation of soybean chalcone synthase genes in plant defence, symbiosis and upon environmental stimuli. | four independent recombinant lambda clones hybridizing to parsley chalcone synthase (chs) cdna were isolated from a soybean (glycine max) genomic library. restriction fragment length polymorphism (rflp) analysis indicated that the chs gene family comprises six members. the chs genes were found to be clustered with three genes on a 10 kb segment and pairs on others. dna sequences of the 5'-, the coding-, and the 3' untranslated regions were determined for three different genes. a consensus alignm ... | 1989 | 2476656 |
| isolation and partial characterization of the cell membrane of walker-256 carcinosarcoma ascites cells. | cell membranes of carcinosarcoma-256 ascites cells were isolated by the zn++ method. neutral sugars, sialic acid, fucose and n-acetylhexosamines were determined in the cell membrane fraction. cells were agglutinated by wheat germ agglutinin, concanavalin a, and the lectins from phaseolus vulgaris and glycine max. no agglutination was observed with peanut lectin (arachis hypogaea). gel electrophoresis under dissociating conditions revealed more than 40 bands in the membrane fraction after silver ... | 1989 | 2486452 |
| phytoalexin synthesis in soybean cells: elicitor induction of reductase involved in biosynthesis of 6'-deoxychalcone. | chromatofocusing on mono p proved to be an efficient purification procedure for the nadph-dependent reductase from soybean (glycine max l.) cell cultures which acts together with chalcone synthase in the biosynthesis of 2',4',4-trihydroxychalcone (6'-deoxychalcone). by isoelectric focusing the pi of reductase was determined to be 6.3. addition of pure soybean reductase to cell-free extracts from stimulated cell cultures of parsley and bean (phaseolus vulgaris) and from young flowers of dahlia va ... | 1989 | 2500065 |
| detection of desmethyldiazepam and diazepam in brain of different species and plants. | recent data suggest that desmethyldiazepam (dd), a major metabolite of several benzodiazepines (bzd), might be of natural origin. therefore we tried to quantify dd and diazepam (d) in animals during maturation (e.g. hen, chicken, eggs), in brain of species at different evolutionary stages e.g. salmon, frog, monitor/reptile, rat, cat, dog, deer, bovine) including newborn and adult humans. since low concentrations of dd (range 0.01-0.04 ng/g wet wt) and d (range 0.005-0.02 ng/g) could be measured ... | 1989 | 2502983 |
| regional distribution of glycoconjugates in normal, transitional and neoplastic human colonic mucosa. a histochemical study using lectins. | regional distribution of glycoconjugates in normal and neoplastic colonic mucosa was studied by means of eight lectins: dolichos biflorus (dba), glycine max (sba), triticum vulgare (wga), arachis hypogaea (pna), griffonia simplicifolia-i (gs-i), canavalia ensiformis (con a), limax flavus (lfa), and ulex europaeus-i (uea-i). the lectin binding patterns were examined in 40 normal colonic mucosa (nm) (12 proximal (p) and 28 distal (d], 38 carcinomas (15 p and 23 d), and 31 transitional mucosa (tm) ... | 1989 | 2505446 |
| the microbial ecology of soybean soaking for tempe production. | soybeans soaked in tap water for 24 to 36 h at 20, 30 or 37 degrees c underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/ml (depending on temperature) and a reduction of ph from 6.5 to 4.5. lactobacillus casei, streptococcus faecium, staphylococcus epidermidis and streptococcus dysgalactiae dominated the fermentation but, significant contributions were also made by klebsiella pneumoniae, klebsiella ozaenae, enterobacter cloacae, enterobact ... | 1989 | 2518226 |
| nodule-specific kinases phosphorylating nuclear factors in isolated nuclei. | in vitro phosphorylation of total nuclear proteins from soybean (glycine max l) nodules formed by bradyrhizobium japonicum 61a76 showed several differences in comparison with those from uninfected roots or embryonic-axes nuclei. three types of protein phosphorylations were observed in nodule nuclei: ca(2+)- and calmodulin-independent, ca(2+)- and calmodulin-dependent, and ca(2+)-dependent but calmodulin-independent. in addition, ca(2+)-dependent dephosphorylation of some nuclear proteins was obs ... | 1989 | 2535508 |
| selective staining of a population of parvalbumin-containing gabaergic neurons in the rat cerebral cortex by lectins with specific affinity for terminal n-acetylgalactosamine. | lectins with specific affinity for terminal n-acetylgalactosamine, vicia villosa agglutinin (vva) and glycine max agglutinin (soybean agglutinin; sba), are shown to stain selectively a subpopulation of gabaergic neurons in the rat cerebral cortex. about 90% of vaa- and/or sba-stained cells are also parvalbumin-immunoreactive, but no vva-stained cells showed somatostatin-28-like or cholecystokinin-8-like immunoreactivities. | 1989 | 2565147 |
| dynamic changes of cell-surface glycoconjugates in human palmar epidermis following friction-blisters. | damage and repair of cell-surface glycoconjugates were examined in human palmar skin following friction-blister injury, using biotinylated lectins and the avidin-biotin complex method. in normal skin, concanavalin a, ricinus communis, and triticum vulgaris bound to the surface of cells from the basal layer to the granular layer. after injury, binding of concanavalin a was absent in the plasma membrane, but appeared in the cytoplasm at perinuclear sites. the surface reaction was recovered in basa ... | 1989 | 2582482 |
| crystal structure of recombinant human interleukin-1 beta at 2.0 a resolution. | the crystal structure of recombinant human interleukin-1 beta (il-1 beta) has been determined at 2.0 a resolution and refined to a crystallographic r-factor of 0.19. three heavy-atom derivatives were identified and used for multiple isomorphous replacement phasing. interpretation of the resulting electron density map revealed a structure in which there are 12 antiparallel beta-strands and no alpha-helix. the single 153-residue polypeptide chain is folded into a six-stranded beta-barrel similar i ... | 1989 | 2585509 |
| characterization of a neuraminidase from corynebacterium aquaticum responsible for th polyagglutination. | th polyagglutinability is characterized by the agglutination of the red blood cells (rbc) by arachis hypogaea, medicago disciformis, vicia cretica but, in contrast to the t phenomenon, not by glycine max (glycine soja). because th transformation of rbc has been obtained in vitro, the mechanism of th polyagglutinability expression has been studied and reproduced experimentally. an enzyme with neuraminidase specificity has been isolated from the culture supernatant of corynebacterium aquaticum, an ... | 1989 | 2617954 |
| lectin binding to small intestinal goblet cells of newborn, suckling, and weaned pigs. | lectin binding of small intestinal goblet cells was examined in newborn, suckling, and weaned pigs. sections of duodenum, proximal portion of the jejunum, distal portion of the jejunum, and ileum were embedded in a hydrophilic acrylic resin and treated with each of the following lectins: canavalia ensiformis, ricinus communis i, glycine max, ulex europaeus i, and triticum vulgaris. percentages of goblet cells binding each lectin were calculated within intestinal regions. differences in lectin-bi ... | 1989 | 2619128 |