| molecular, technological and safety characterization of gram-positive catalase-positive cocci from slightly fermented sausages. | the population of gram-positive catalase-positive cocci from slightly fermented sausages was characterized at species and strain level by molecular techniques and some technological and hygienic aspects were also considered. staphylococcus xylosus was the predominant species (80.8%) followed by staphylococcus warneri (8.3%), staphylococcus epidermidis (5.8%) staphylococcus carnosus (4.6%), and kocuria varians (0.4%). proteolytic activity was observed in 23% of the isolates. the species with the ... | 2006 | 16297478 |
| characterization of sediment bacteria involved in selenium reduction. | bacterial reduction of selenium (se) oxyanions (se[vi] and se[iv]) to elemental se (se[0]) is one of the major biogeochemical processes removing se from agricultural drainage water and depositing se in the sediment. this study was conducted to characterize se-reducing bacterial populations in lost hills evaporation pond sediment and to observe their response to se(vi) and organic c amendments. se(vi) was removed from the dissolved phase in the sediment slurries amended with organic c with a decr ... | 2006 | 16324840 |
| ecology and dynamics of coagulase-negative cocci isolated from naturally fermented italian sausages. | coagulase-negative cocci (cnc) ecology in naturally fermented sausages from friuli venezia giulia region, in the north east of italy, was investigated. a total of 617 cnc strains, isolated from three different plants during the fermentation process, were identified by traditional methods (biochemical tests) and molecular methods based on species specific pcr, pcr-denaturing gradient gel electrophoresis (dgge) and sequencing of the v3 region of the 16s rrna gene. the identification, by using bioc ... | 2006 | 16337767 |
| contagious mucocutaneous dermatitis of the mountain hare (lepus timidus): pathology and cause. | contagious mucocutaneous dermatitis is a frequently encountered disease of mountain hares (lepidus timidus) in finland. we describe the histopathologic changes and propose an etiologic cause for this disorder. fifty-three cases collected during 1982-2000 were examined histologically. transmission electron microscopy was performed in one case. in fully developed lesions, keratinocytes in epidermis and follicular infundibula were swollen and contained large eosinophilic intracytoplasmic inclusion ... | 2005 | 16456167 |
| [cloning of the conserved fragment of urease gene of staphylococcus warneri]. | to obtain the clone of conserved fragment of urease gene of staphylococcus warneri. | 2006 | 16525613 |
| heterogeneous resistance to vancomycin in staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus warneri clinical strains: characterisation of glycopeptide susceptibility profiles and cell wall thickening. | the population analysis profile (pap) method as well as analysis of autolytic activity and cellular ultrastructure by transmission electron microscopy (tem) were used to characterise staphylococcus epidermidis, staphylococcus haemolyticus and staphylococcus warneri clinical strains with reduced susceptibility to glycopeptides. all strains showed heterogeneous profiles to vancomycin and teicoplanin by the pap method. subpopulations that grew in the presence of high concentrations of each drug wer ... | 2006 | 16542825 |
| coagulase-negative staphylococci in very-low-birth-weight infants: inability of genetic markers to distinguish invasive strains from blood culture contaminants. | selected coagulase-negative staphylococci from the blood of very-low-birth-weight infants in the neonatal intensive care unit at the royal women's hospital, melbourne, collected over a 5-year period were examined. isolates were classified as invasive or contaminants, speciated, typed by pulsed-field gel electrophoresis, and examined for biofilm genes (icaa, icac, and icad), adhesion genes (atle, fbe), and the number of copies of is256. of the 24 isolates studied, there were 13 contaminants and 1 ... | 2006 | 16598472 |
| eggshell factors influencing eggshell penetration and whole egg contamination by different bacteria, including salmonella enteritidis. | trans-shell infection routes and whole egg contamination of 7 selected bacterial strains; staphylococcus warneri, acinetobacter baumannii, alcaligenes sp., serratia marcescens, carnobacterium sp., pseudomonas sp. and salmonella enteritidis, recovered from egg contents, were studied. the first objective was to correlate bacterial eggshell penetration with various eggshell characteristics and bacterial strains. an agar approach was used to assess the eggshell penetration. the second objective was ... | 2006 | 16822571 |
| microbiological study of cosmetic products during their use by consumers: health risk and efficacy of preservative systems. | to evaluate the microbial contamination of 91 cosmetics (23 o/w emulsions, 47 tensiolytes, 21 aqueous pastes) in three different states of use (intact, in-use, ending product) and the protection efficacy of the preservative systems most frequently used in the analysed cosmetic formulations. | 2006 | 16910936 |
| microbiosensors for measurement of microbially available dissolved organic carbon: sensor characteristics and preliminary environmental application. | microbial respiration-based microbiosensors used for quantification of available dissolved organic carbon (adoc) instantaneously respired by microorganisms are described. the sensing membranes contained aerobic seawater microorganisms immobilized in a polyurethane hydrogel. molecular investigations revealed that the bacterial strain used was most closely related to staphylococcus warneri. this strain was characterized by low substrate selectivity, which was reflected in the response to various m ... | 2006 | 16936058 |
| meningoencephalitis associated with staphylococcus warneri in a dog. | a case of meningoencephalitis in a dog caused by staphylococcus warneri is reported here. the history and clinical signs were suggestive of possible central nervous system infection. analysis of cerebrospinal fluid documented a neutrophilic pleocytosis (890 cells/mul) and the presence of occasional intracellular cocci. staphylococcus warneri was isolated from the microbiological culture of the cerebrospinal fluid. treatment consisted of intravenous antibiotics, supportive care and anticonvulsant ... | 2006 | 17004952 |
| lysis of staphylococcal mastitis pathogens by bacteriophage phi11 endolysin. | the staphylococcus aureus bacteriophage phi11 endolysin has two peptidoglycan hydrolase domains (endopeptidase and amidase) and an sh3b cell wall-binding domain. in turbidity reduction assays, the purified protein can lyse untreated staphylococcal mastitis pathogens, staphylococcus aureus and coagulase-negative staphylococci (staphylococcus chronogenes, staphylococcus epidermidis, staphylococcus hyicus, staphylococcus simulans, staphylococcus warneri and staphylococcus xylosus), making it a stro ... | 2006 | 17054440 |
| bacteraemia in patients with hidradenitis suppurativa undergoing carbon dioxide laser surgery: detection and quantification of bacteria by lysis-filtration. | hidradenitis suppurativa (hs) is a cicatrising and persistent disease of apocrine gland-bearing areas in adults. the severity of this condition varies from a few suppurating lesions to widespread, disabling disease. the aetiology is obscure, but suggested contributory factors include a genetic predisposition, comedones occluding the pilosebaceous apparatus, bacterial infections, and hormonal factors. treatment consists mainly of surgery, while medical therapies serve principally as adjunct thera ... | 2006 | 17135736 |
| is21-558 insertion sequences are involved in the mobility of the multiresistance gene cfr. | during a study of florfenicol-resistant porcine staphylococci from denmark, the genes cfr and fexa were detected in the chromosomal dna or on plasmids of staphylococcus hyicus, staphylococcus warneri, and staphylococcus simulans. a novel variant of the phenicol resistance transposon tn558 was detected on the ca. 43-kb plasmid pscfs6 in s. warneri and s. simulans isolates. sequence analysis of a 22,010-bp segment revealed that the new tn558 variant harbored an additional resistance gene region in ... | 2007 | 17145796 |
| the staphostatin family of cysteine protease inhibitors in the genus staphylococcus as an example of parallel evolution of protease and inhibitor specificity. | staphostatins constitute a family of staphylococcal cysteine protease inhibitors sharing a lipocalin-like fold and a unique mechanism of action. each of these cytoplasmic proteins is co-expressed from one operon, together with a corresponding target extracellular cysteine protease (staphopain). to cast more light on staphostatin/staphopain interaction and the evolution of the encoding operons, we have cloned and characterized a staphopain (stpa2aur ch-91) and its inhibitor (stpina2aur ch-91) fro ... | 2007 | 17261086 |
| identification of diazotrophs in the culturable bacterial community associated with roots of lasiurus sindicus, a perennial grass of thar desert, india. | lasiurus sindicus is a highly nutritive, drought-tolerant, perennial grass that is endemic to the thar desert of rajasthan, india. analysis of 16s rrna coding genes of the bacterial isolates enriched in nitrogen-free semisolid medium, from the surface-sterilized roots of l. sindicus, showed predominance of gram-negative over gram-positive bacteria. according to comparative sequence analysis of 16s rdna sequence data, gram-positive bacteria with low gc content (staphylococcus warneri and bacillus ... | 2007 | 17264993 |
| prevalence and clinical relevance of staphylococcus warneri in the neonatal intensive care unit. | to describe the prevalence of staphylococcus warneri on the hands of nurses and the clinical relevance of this organism among neonates in the neonatal intensive care unit (nicu). | 2007 | 17326024 |
| antibiotic resistance patterns of coagulase-negative staphylococcus strains isolated from blood cultures of septicemic patients in turkey. | the aim of this study is to determine antibiotic resistance patterns and slime production characteristics of coagulase-negative staphylococci (cons) caused nosocomial bacteremia. a total of 200 cons strains were isolated from blood samples of patients with true bacteremia who were hospitalized in intensive care units and in other departments of istanbul university cerrahpasa medical hospital between 1999 and 2006. among 200 cons isolates, staphylococcus epidermidis was the most prevalent species ... | 2009 | 17475456 |
| antimicrobial resistance patterns of colonizing flora on nurses' hands in the neonatal intensive care unit. | the centers for disease control and prevention recommends the use of an alcohol-based handrub for health care worker hand hygiene. the purpose of this study was to examine effects of hand hygiene product and skin condition on the antimicrobial resistance patterns of colonizing hand flora among nurses. | 2007 | 17482994 |
| lantibiotic engineering: molecular characterization and exploitation of lantibiotic-synthesizing enzymes for peptide engineering. | lanthionine-containing peptide antibiotics called lantibiotics are produced by a large number of gram-positive bacteria. nukacin isk-1 produced by staphylococcus warneri isk-1 is type-a(ii) lantibiotic. ribosomally synthesized nukacin isk-1 prepeptide (nuka) consists of an n-terminal leader peptide followed by a c-terminal propeptide moiety that undergoes several post-translational modification events including unusual amino acid formation by the modification enzyme nukm, cleavage of leader pept ... | 2007 | 17827974 |
| evolution of slime production by coagulase-negative staphylococci and enterotoxigenic characteristics of staphylococcus aureus strains isolated from various human clinical specimens. | the present study was designed to determine the slime production of coagulase-negative staphylococci (cons) and the enterotoxigenic properties of staphylococcus aureus strains, and to evaluate the clinical importance of slime-producing cons and enterotoxigenic s. aureus strains isolated from various human clinical specimens. for this purpose, a total of 120 staphylococcus strains were isolated and identified, and further characterized for their slime production and enterotoxigenicity. of the cli ... | 2007 | 17893164 |
| development of a real-time pcr assay for the detection and identification of staphylococcus capitis, staphylococcus haemolyticus and staphylococcus warneri. | staphylococcus capitis, staphylococcus haemolyticus and staphylococcus warneri are coagulase-negative staphylococci. each species has different characteristics, and a difference in pathology is also seen in compromised hosts. therefore, the development of a species-specific simple detection method for the identification of these staphylococci is important. here, a species-specific real-time pcr assay is reported that targets the superoxide dismutase a-encoding gene of these bacteria. primers wer ... | 2007 | 17893172 |
| cooperative transport between nukfeg and nukh in immunity against the lantibiotic nukacin isk-1 produced by staphylococcus warneri isk-1. | nukacin isk-1 is a lantibiotic produced by staphylococcus warneri isk-1. previous studies have reported that the self-protection system of the nukacin isk-1 producer involves the cooperative function of the abc transporter nukfeg and the lantibiotic-binding immunity protein nukh. in this study, the cooperative mechanism between nukfeg and nukh was characterized by using fluorescein-4-isothiocyanate (fitc)-labeled nukacin isk-1 (fitc-nuk) to clarify the localization of nukacin isk-1 in the immuni ... | 2008 | 17951378 |
| diagnostic evidence of staphylococcus warneri as a possible cause of bovine abortion. | following a routine necropsy of a bovine fetus aborted at 5 months of gestation, placenta, fetal tissue samples, and stomach contents were subjected to a number of laboratory tests. staphylococcus warneri was isolated in pure culture from the lung, liver, and stomach contents, whereas the placenta yielded s. warneri and a number of contaminants. gross evaluation of agar plates showed predominant colonies to be morphologically consistent with those of s. warneri and the identity of the agent was ... | 2007 | 17998560 |
| occurrence of staphylococci in the oral cavities of healthy adults and nasal oral trafficking of the bacteria. | to investigate a possible peroral route of infective endocarditis (ie), the occurrence of staphylococci in the oral cavity was examined using saliva and supragingival plaque specimens from 56 systemically and periodontally healthy adults aged 22-43 years old (27.1+/-5.3). nine staphylococcus species and 334 isolates were identified. in saliva, the total occurrence rate was 83.9 % and the total number of bacteria was 10(2)-10(4) c.f.u. ml(-1). staphylococcus aureus was the most frequent species ( ... | 2008 | 18065673 |
| characterization of anti-legionella activity of warnericin rk and delta-lysin i from staphylococcus warneri. | legionella pneumophila, the causative agent of legionnaires' disease, is a waterborne bacteria. it can multiply in man-made water systems and infect people who inhale contaminated droplets. we have previously reported a staphylococcus warneri strain that display an anti-legionella activity. in this work, we characterized three anti-legionella peptides that are produced by s. warneri. one peptide, warnericin rk, is original, while the two others are delta-lysin i and delta-lysin ii, whose genes w ... | 2008 | 18339450 |
| molecular properties of the putative autolysin atl(wm) encoded by staphylococcus warneri m: mutational and biochemical analyses of the amidase and glucosaminidase domains. | the putative autolysin atl(wm) of staphylococcus warneri m is a modular protein exhibiting two enzyme activities, an n-terminal side amidase (ami(atlwm)-r1-r2) and a c-terminal side glucosaminidase (r3-glu(atlwm)). zymographic analysis of the protein overproduced in escherichia coli showed that both enzymes were active toward 17 gram-positive bacteria, including staphylococci, lactobacilli, lactococci, enterococci, and micrococci. the purified enzyme core ami(atlwm) (or glu(atlwm)) had the ph an ... | 2008 | 18440165 |
| microbial cultures of the microkeratome blade immediately after flap construction in laser in situ keratomileusis. | to assess the incidence of positive cultures from samples of the microkeratome blade after routine laser in situ keratomileusis (lasik) procedures as a measure of the risk for postoperative infectious keratitis. | 2008 | 18471644 |
| constituents with independence from growth temperature for bacteria using pyrolysis-gas chromatography/differential mobility spectrometry with analysis of variance and principal component analysis. | four bacteria, escherichia coli, pseudomonas aeruginosa, staphylococcus warneri, and micrococcus luteus, were grown at temperatures of 23, 30, and 37 degrees c and were characterized by pyrolysis-gas chromatography/differential mobility spectrometry (py-gc/dms) providing, with replicates, 120 data sets of retention time, compensation voltage, and ion intensity, each for negative and positive polarity. principal component analysis (pca) for 96 of these data sets exhibited clusters by temperature ... | 2008 | 18493677 |
| an escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of autodegradation. | v8 protease (gluv8), a member of the glutamyl endopeptidase i family isolated from the v8 strain of staphylococcus aureus, is widely used for proteome analysis because of its unique substrate specificity and resistance to detergents. we recently developed an escherichia coli expression system for the production of gluv8 based on a technique that suppresses the autoproteolysis--the use of the prosequence of its homologue (gluse) from staphylococcus epidermidis as a chimeric form or the introducti ... | 2008 | 18616923 |
| lymphoepithelioma-like carcinoma of the urinary bladder in a cow associated with bovine papillomavirus type-2. | lymphoepithelioma-like carcinoma (lelca) of the urinary bladder is reported in a 7-year-old cow that had grazed pasture rich in bracken fern and had suffered from severe intermittent haematuria from 3 to 4 years of age. on necropsy examination there were multiple haemorrhagic foci scattered over the mucosal surface of the urinary bladder. microscopically there were nests, cords and sheets of neoplastic cells infiltrating the lamina propria and muscularis propria. these had a syncytial appearance ... | 2008 | 18675990 |
| characterisation of aerobically grown non-spore-forming bacteria from paper mill pulps containing recycled fibres. | a total of 179 non-spore-forming bacteria aerobically growing on nutrient agar, plate count agar or in specific enrichment conditions for salmonella, campylobacteria, listeria, yersinia or staphylococci, were isolated from 16 untreated paper mill pulps. after phenotypical screening the isolates were characterised by automated ribotyping and partial sequencing of the 16s rrna gene. they could be divided into seven taxonomical classes representing 63 taxa (species): actinobacteria (11 species), ba ... | 2009 | 18820960 |
| prevalence and persistence of coagulase-negative staphylococcus species in three dairy research herds. | coagulase-negative staphylococcus species (cns) were isolated from 11.3% (1407 of 12,412) of mammary quarter milk samples obtained from cows in three dairy research herds in 2005. approximately 27% (383/1407) of cns was identified to the species level. the species distribution among those cns identified from all herds was staphylococcus chromogenes (48%), staphylococcus hyicus (26%), staphylococcus epidermidis (10%), staphylococcus simulans (7%), staphylococcus warneri (2%), staphylococcus homin ... | 2009 | 18950962 |
| binding specificity of the lantibiotic-binding immunity protein nukh. | nukh is a lantibiotic-binding immunity protein that shows strong binding activity against type a(ii) lantibiotics. in this study, the binding specificity of nukh was analyzed by using derivatives of nukacin isk-1, which is a type a(ii) lantibiotic produced by staphylococcus warneri isk-1. interactions between cells of lactococcus lactis transformants expressing nukh and nukacin isk-1 derivatives were analyzed by using a quantitative peptide-binding assay. differences in the cell-binding rates of ... | 2008 | 18978082 |
| determination of three amino acids causing alteration of proteolytic activities of staphylococcal glutamyl endopeptidases. | staphylococcus aureus, staphylococcus epidermidis, and staphylococcus warneri secrete glutamyl endopeptidases, designated gluv8, gluse, and glusw, respectively. the order of their protease activities is gluse < glusw << gluv8. in the present study, we investigated the mechanism that causes these differences. expression of chimeric proteins between gluv8 and gluse revealed that the difference is primarily attributed to amino acid residues 170-195, which define the intrinsic protease activity, and ... | 2009 | 19090719 |
| performance of api staph id 32 and staph-zym for identification of coagulase-negative staphylococci isolated from bovine milk samples. | in this study, the accuracy of two phenotypic tests, api staph id 32 and staph-zym, was determined for identification of coagulase-negative staphylococci (cns) from bovine milk samples in comparison with identification based on dna-sequencing. a total of 172 cns isolated from bovine milk were classified into 17 species. the most frequently isolated species based on rpob sequencing were staphylococcus chromogenes and staphylococcus epidermidis, followed by staphylococcus xylosus, staphylococcus w ... | 2009 | 19110383 |
| sterility screening of platelet concentrates: questioning the optimal test strategy. | routine bacterial monitoring of apheresis platelet concentrates (apc) and pooled platelet concentrates (ppc) was introduced in two german blood services using culture and real-time reverse transcriptase (rt)-polymerase chain reaction (pcr). the results of testing are reviewed and used to discuss different strategies for detection of bacterial contamination of pcs. | 2008 | 19121182 |
| response to somatic cell count-based selection for mastitis resistance in a divergent selection experiment in sheep. | a divergent selection experiment in sheep was implemented to study the consequences of log-transformed somatic cell score (scs)-based selection on resistance to natural intramammary infections. using dams and progeny-tested rams selected for extreme breeding values for scs, we created 2 groups of ewes with a strong divergence in scs of approximately 3 genetic standard deviations. a survey of 84 first-lactation ewes of both the high and low scs lines indicated favorable responses to scs-based sel ... | 2009 | 19233814 |
| identification and characterization of a bioactive lantibiotic produced by staphylococcus warneri. | lantibiotics are a group of potent antibacterial agents that contain unusual amino acids, such as the thioether amino acids lanthionine and methyllanthionine, and the didehydroamino acids didehydroalanine and didehydro-aminobutyric acid. here, we report on an antibacterial lantibiotic peptide named swlp1 (staphylococcus warneri lantibiotic peptide 1), which is secreted from staphylococcus warneri (deposited with dsmz, accession number dsm 16081). swlp1 was purified from growth media. the purifie ... | 2009 | 19361283 |
| molecular properties of the glucosaminidase acma from lactococcus lactis mg1363: mutational and biochemical analyses. | the major autolysin acma of lactococcus lactis ssp. cremoris mg1363 is a modular protein consisting of an n-terminal signal sequence, a central enzymatic region (glu(acma) as a glucosaminidase), and a c-terminal cell-recognition domain (lysm123). glu(acma) (about 160 amino acids) belongs to the glycoside hydrolase (gh) 73 family, and the two acidic residues e128 and d153 have been thought to be catalytically important. in this study, amino-acid substitution analysis of acma was first carried out ... | 2009 | 19686822 |
| interaction of sortase a and lipase 2 in the inhibition of staphylococcus aureus biofilm formation. | recombinant sortase a (srta) was used to immune rabbit, and the inhibitory activity of anti-srta serum on staphylococcus aureus biofilm formation was tested. biofilm formation was inhibited by anti-srta rabbit serum in s. aureus atcc25923 and two clinical isolated strains. the antiserum was separated into two fractions, and the main component with the inhibitory activity was demonstrated to be the igg fraction. two proteins interact with the igg fraction were identified by using an in vitro pull ... | 2009 | 19838678 |
| single-step purification of different lipases from staphylococcus warneri. | three different lipases from the extract crude of staphylococcus warneri have been purified by specific lipase-lipase interactions using different lipases (tll, rml, pfl, btl2) covalently attached to a solid support as adsorption matrix. btl2 immobilized on glyoxyl-dtt adsorbed selectivity only a 30 kda lipase from the crude, which was desorbed by adding 0.1% triton x-100. using glyoxyl-pfl as matrix, two new lipases (28 and 40 kda) were adsorbed, and completely pure 40 kda lipase was obtained a ... | 2010 | 19954784 |
| staphylococcus warneri ventriculoperitoneal shunt infection: failure of diagnosis by ventricular csf sampling. | the definite diagnosis of hydrocephalus valve infection is generally made by cerebrospinal fluid (csf) sampling via the valve reservoir, which is considered to be more dependable than that of the csf obtained by lumbar puncture. | 2010 | 20013125 |
| maldi-tof mass spectrometry speciation of staphylococci and their discrimination from micrococci isolated from indoor air of schoolrooms. | the focus of our work is the identification of medically relevant staphylococci from the environment; these organisms are among the major opportunistic pathogens associated with human disease. andersen sampling was performed in schoolrooms during the school year. eleven of thirty six isolates (all gram-positive tetrads) were identified as staphylococci and 23 were characterized as micrococci. maldi-tof ms profiling was used as the first stage in the classification followed by standard biochemica ... | 2010 | 20383373 |
| polymerase chain reaction detection of enterotoxins genes in coagulase-negative staphylococci isolated from brazilian minas cheese. | for a long time, staphylococcus aureus has been always thought to be the only pathogenic species among staphylococcus, while coagulase-negative staphylococci (cns) were classified as contaminant agents. however, molecular techniques have shown that these microorganisms also possess enterotoxin-encoding genes. the aim of this study was to analyze the frequency of genes for staphylococcal enterotoxins sea, seb, sec, and sed in cns strains isolated from minas soft cheese and to assess the in vitro ... | 2010 | 20528174 |
| activity of telavancin and comparator antimicrobial agents tested against staphylococcus spp. isolated from hospitalised patients in europe (2007-2008). | the activity of telavancin was evaluated against staphylococcus spp. collected from european hospitals as part of an international surveillance study (2007-2008). a total of 7534 staphylococcal clinical isolates [5726 staphylococcus aureus and 1808 coagulase-negative staphylococci (cons)] were included. isolates were tested for susceptibility according to reference methods and minimum inhibitory concentration (mic) values were interpreted based on clinical and laboratory standards institute (cls ... | 2010 | 20598860 |
| staphylococcus warneri meningitis in a patient with strongyloides stercoralis hyperinfection and lymphoma: first report of a case. | a case of meningitis due to staphylococcus warneri in a patient with a hyperinfection with strongyloides stercoralis possibly associated with rituximab treatment for mantel cell lymphoma is reported for the first time in the literature. the patient was a 59-year-old woman, with a 3-year history of an apparently well controlled lymphoma after treatment with chemotherapy-immunotherapy and then immunotherapy alone, and diagnosis of strongyloidiasis. meningitis was diagnosed by cerebrospinal fluid c ... | 2010 | 20602029 |
| nukacin 3299, a lantibiotic produced by staphylococcus simulans 3299 identical to nukacin isk-1. | nukacin 3299 (formerly designated simulancin 3299), produced by a staphylococcus simulans strain involved in bovine mastitis in brazil, is the first peptide bacteriocin described in this staphylococcal species. with the intent to elucidate some aspects of its biology, nukacin 3299 was purified and characterized. the mass of the purified bacteriocin was shown to be 2957.3 da, and the peptide n-terminal amino acids (kkksgvi) were identified by edman degradation. the nukacin 3299 structural gene, n ... | 2010 | 20627619 |
| rapid differentiation of staphylococcus aureus, staphylococcus epidermidis and other coagulase-negative staphylococci and meticillin susceptibility testing directly from growth-positive blood cultures by multiplex real-time pcr. | this study evaluated a multiplex real-time pcr method specific for the meca, fema-sa and fema-se genes for rapid identification of staphylococcus aureus, staphylococcus epidermidis and non-s. epidermidis coagulase-negative staphylococci (cons), and meticillin susceptibility testing directly in positive blood cultures that grew gram-positive cocci in clusters. a total of 100 positive blood cultures produced: 39 s. aureus [12 meticillin-resistant s. aureus (mrsa), 31% of all the s. aureus]; 30 s. ... | 2010 | 20813851 |
| rapid identification of staphylococci from prosthetic joint infections using maldi-tof mass-spectrometry. | hospital-acquired infections associated with implanted medical devices are most commonly caused by staphylococci. current methods of species identification are slow, costly, and sometimes unreliable. we evaluated the ability of a bruker daltonics microflex maldi-tof/ms in conjunction with maldi biotyper software to identify 158 characterized staphylococcal isolates from prosthetic joint infections, including 36 staphylococcus aureus, 100 staphylococcus epidermidis, 10 staphylococcus capitis, 8 s ... | 2010 | 20963723 |
| characterization of 26 staphylococcus warneri isolates from orthopedic infections. | staphylococcus warneri is a coagulase negative staphylococcus (cns) commonly present in the flora of human epithelia and mucosal membranes. over the last two decades, similarly to other cns species, s. warneri has been reported as a new emerging pathogen, capable of causing serious infections usually in association with the presence of implant materials, but, at times, even in the absence of a foreign body and in patients considered immunocompetent. at present, there is still a lack of scientifi ... | 2010 | 20963724 |
| safety-related properties of staphylococci isolated from food and food environments. | to test some safety-related properties within 321 staphylococci strains isolated from food and food environments. | 2010 | 21143714 |
| ovarian hydrobursitis in female camels (camelus dromedaries): biochemical, bacterial and protozoal evaluation. | the aim of this study was to evaluate female camels affected with ovarian hydrobursitis (n = 31) for hematological and biochemical findings and for bacterial and protozoal infections. blood samples were obtained and surgical ablation of the affected bursa was performed. bursal fluid, follicular fluid, and serum were subjected to hormonal and biochemical analyses. bursal fluids were cultured and colonies were identified using biomérieux vitek two compact system. passive haemagglutination test was ... | 2010 | 21144565 |
| effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan. | chitosan is a biopolymer with antimicrobial activity and film-forming properties. in this study, the effects on salmonella shell contamination and trans-shell penetration of coating hens' eggs with chitosan was evaluated. a chitosan was selected from eight types (four non-commercial and four commercial) based on its antimicrobial activity against salmonella enterica serovar enteritidis (s. enteritidis). for this purpose, a contact plate method was developed and chitosans were applied at a concen ... | 2010 | 21146239 |
| differentiation of staphylococcus spp. by high-resolution melting analysis. | high-resolution melting analysis (hrma) is a fast (post-pcr) high-throughput method to scan for sequence variations in a target gene. the aim of this study was to test the potential of hrma to distinguish particular bacterial species of the staphylococcus genus even when using a broad-range pcr within the 16s rrna gene where sequence differences are minimal. genomic dna samples isolated from 12 reference staphylococcal strains (staphylococcus aureus, staphylococcus capitis, staphylococcus caprae ... | 2010 | 21164574 |
| fatty acid composition modulates sensitivity of legionella pneumophila to warnericin rk, an antimicrobial peptide. | warnericin rk is an antimicrobial peptide, produced by a staphyloccocus warneri strain, described to be specifically active against legionella, the pathogenic bacteria responsible for legionnaires' disease. warnericin rk is an amphiphilic alpha-helical peptide, which possesses a detergent-like mode of action. two others peptides, δ-hemolysin i and ii, produced by the same s. warneri strain, are highly similar to s. aureus δ-hemolysin and also display anti-legionella activity. it has been recentl ... | 2010 | 21182824 |
| characterization of the histidine decarboxylase gene of staphylococcus epidermidis tyh1 coded on the staphylococcal cassette chromosome. | histamine production from histidine in fermented food results in food spoilage, and is harmful to consumers. from fish-miso, we have isolated a new bacterial strain staphylococcus epidermidis tyh1, which produced histamine under acidic condition in the medium supplemented with glucose. using oligonucleotides deduced from the histidine decarboxylase gene (hdca) of lactobacillus hilgardii, about 14-kbp dna region of the tyh1 genome was cloned and sequenced. this region contained two putative genes ... | 2011 | 21236322 |
| an unusual presentation of endocarditis caused by staphylococcus warneri. | staphylococcus warneri does not generally cause serious infections in humans. we report a case of endocarditis in a healthy individual with no known past medical history. s. warneri was identified in her blood cultures and echocardiographic evidence confirmed the diagnosis of bacterial endocarditis. there was no apparent cause for her infection, and risk factors such as invasive treatment or medical implant were not present. this rare clinical presentation illustrates the importance of not overl ... | 2010 | 21258573 |
| optimization of lipase production by staphylococcus warneri ex17 using the polydimethylsiloxanes artificial oxygen carriers. | in this research, the combined effects of polydimethylsiloxane (pdms) and different conditions of oxygen volumetric mass transfer coefficient (k(l)a) on lipase production by staphylococcus warneri ex17 were studied and optimized in bioreactor cultures. raw glycerol from biodiesel synthesis was used as the sole carbon source. full-factorial central composite design and the response surface methodology were employed for the experimental design and analysis of the results. the optimal polydimethyls ... | 2011 | 21340482 |
| identification and characterization of potential autochthonous starter cultures from a croatian "brand" product "slavonski kulen". | the microbial population of a traditional croatian fermented sausage "slavonski kulen" was isolated, identified and subjected to technological and functional characterization in order to select potential autochthonous functional starter cultures. dominant microflora were lactic acid bacteria (lab), followed by staphylococci. identification of isolated lactobacilli showed domination of leuconostoc mesenteroides and lactobacillus acidophilus while staphylococcus xylosus and staphylococcus warneri ... | 2011 | 21377808 |
| focal intracranial infections due to actinomyces species in immunocompetent patients: diagnostic and therapeutic challenges. | actinomyces spp. are often underestimated as causes of central nervous system infection because they are not looked for routinely and are difficult to detect. we try to determine the optimal management of these problematic intracranial infectious diseases in neurosurgical practice. | 2010 | 21492568 |
| purification, immobilization, and characterization of a specific lipase from staphylococcus warneri ex17 by enzyme fractionating via adsorption on different hydrophobic supports. | staphylococcus warneri strain ex17 produces three lipases with different molecular weights of 28, 30, and 45 kda. the 45 kda fraction (swl-45) has been purified from crude protein extracts by one chromatographic step based on the selective adsorption of this lipase by interfacial activation on different hydrophobic supports at low ionic strength. the adsorption of swl-45 on octyl-sepharose increased the enzyme activity by 60%, but the other lipases were also adsorbed on this support. using butyl ... | 2011 | 21509953 |
| recurrent staphylococcus warnerii prosthetic valve endocarditis : a case report and review. | abstract: to our knowledge, there have been only six s. warneri endocarditis cases reported in the english-language literature (medline: 1966 to april 2011).we report a case of recurrent s. warneri endocarditis in a patient with prosthetic valve and silicon mammoplasty and we also review the relevant literature. | 2011 | 21513546 |
| molecular detection of enterotoxins e, g, h and i in staphylococcus aureus and coagulase-negative staphylococci isolated from clinical samples of newborns in brazil. | aims:ôçé the objective of this study was to investigate the detection of see, seg, seh and sei in strains of staphylococcus aureus and coagulase-negative staphylococci (cns) using rt-pcr. methods and results:ôçé in this study, 90 staph.┬áaureus strains and 90 cns strains were analysed by pcr for the detection of genes encoding staphylococcal enterotoxins (se) e, g, h and i. one or more genes were detected in 54 (60%) staph.┬áaureus isolates and in 29 (32┬à2%) cns isolates. staphylococcus epiderm ... | 2011 | 21672099 |
| validation of the microscan-96 for the species identification and methicillin susceptibility testing of clinical significant coagulase-negative staphylococci. | an automated system, microscan walkaway-96, in conjugation with combo pos® 28 panels, was validated for the identification and methicillin susceptibility of coagulase-negative staphylococci (cns). the performance of this system was evaluated on 428 cns. identification results were compared using a validated in-house method. methicillin susceptibility was compared with oxacillin mic testing and the presence of the meca gene by pcr (in-house real-time method). the microscan system correctly identi ... | 2011 | 21814758 |
| Microbial diversity in the midguts of field and lab-reared populations of the European corn borer Ostrinia nubilalis. | BACKGROUND: Insects are associated with microorganisms that contribute to the digestion and processing of nutrients. The European Corn Borer (ECB) is a moth present world-wide, causing severe economical damage as a pest on corn and other crops. In the present work, we give a detailed view of the complexity of the microorganisms forming the ECB midgut microbiota with the objective of comparing the biodiversity of the midgut-associated microbiota and explore their potential as a source of genes an ... | 2011 | 21738787 |
| enhanced production of nukacin d13e in lactococcus lactis nz9000 by the additional expression of immunity genes. | nukacin d13e (d13e) is a variant of type-a(ii) lantibiotic nukacin isk-1 produced by staphylococcus warneri isk-1. d13e exhibited a twofold higher specific antimicrobial activity than nukacin isk-1 against a number of gram-positive bacteria. we previously reported the heterologous production of d13e in lactococcus lactis nz9000 under the control of nisin-controlled gene expression system. in this study, we demonstrated enhanced production of d13e by the additional expression of immunity genes, n ... | 2011 | 21904816 |
| role of lipid ii and membrane thickness in the mechanism of action of the lantibiotic bovicin hc5. | lantibiotics are antimicrobial peptides produced by gram-positive bacteria, nisin being the most well-known member. nisin inhibits peptidoglycan synthesis and forms pores at sensitive membranes upon interaction with lipid ii, the essential bacterial cell wall precursor. bovicin hc5, a bacteriocin produced by streptococcus bovis hc5, has the putative n-terminal lipid ii binding motif, and we investigated the mode of action of bovicin hc5 using both living bacteria and model membranes, with specia ... | 2011 | 21876041 |
| growth and effect of staphylococci and lactic acid bacteria on unsaturated free fatty acids. | the growth and the effects of four species of staphylococci and six lactic acid bacteria (lab) of the genus carnobacterium, lactobacillus and pediococcus on unsaturated free fatty acids were studied. the strains were grown in complex medium supplemented either with oleic, linoleic or linolenic acids. growth was followed and oxidation of the substrates measured by tbars. the strains of staphylococcus xylosus 873, 16, staphylococcus warneri 863 and staphylococcus saprophyticus grew well on all the ... | 2000 | 22063710 |
| culturable gut microbiota diversity in zebrafish. | the zebrafish (danio rerio) is an increasingly used laboratory animal model in basic biology and biomedicine, novel drug development, and toxicology. the wide use has increased the demand for optimized husbandry protocols to ensure animal health care and welfare. the knowledge about the correlation between culturable zebrafish intestinal microbiota and health in relation to environmental factors and management procedures is very limited. a semi-quantitative level of growth of individual types of ... | 2012 | 22428747 |
| complete genome of the solvent-tolerant staphylococcus warneri strain sg1. | staphylococcus warneri is a gram-positive bacterium commonly found in human skin flora. the genome of a laboratory s. warneri isolate, strain sg1, was sequenced to explore its mechanism of solvent tolerance and its potential as a chassis for biofuel production. | 2013 | 23516183 |
| analysis of bacterial communities associated with potting media. | potting media are commonly used by growers in different parts of the world for potted plants, raising seedlings and for improving soil characteristics. this study was conducted to characterize bacterial communities occurring in 13 commercial potting media products originating from seven countries. | 2016 | 26844021 |
| bacterial flora concurrent with helicobacter pylori in the stomach of patients with upper gastrointestinal diseases. | to investigate the non-helicobacter pylori (h. pylori) bacterial flora concurrent with h. pylori infection. | 2012 | 22468090 |
| a study of pre-operative presence of micro-organisms in affected knee joints of rheumatoid arthritis patients who need total knee arthroplasty. | to evaluate, by way of intraoperative tissue culture and pathological study, the pre-operative presence of micro-organisms in knee joints of patients with rheumatoid arthritis (ra) who need total knee arthroplasty (tka). | 2017 | 27919672 |
| microbiological stability of canned tuna produced in italy and in non-european countries. | the results of a study on the microbiological stability of canned tuna produced by italian companies and similar canned products manufactured in countries outside europe are reported herein. the study involved 38 samples of canned tuna of various brands, of which 14 were produced by companies outside europe and 24 by italian companies. qualitative and quantitative microbiological tests were conducted for the following parameters: bacterial colony counts at 30°c, total coliforms, total enterobact ... | 2015 | 27800383 |
| induction of invertebrate larval settlement; different bacteria, different mechanisms? | recruitment via settlement of pelagic larvae is critical for the persistence of benthic marine populations. for many benthic invertebrates, larval settlement occurs in response to surface microbial films. larvae of the serpulid polychaete hydroides elegans can be induced to settle by single bacterial species. until now, only pseudoalteromonas luteoviolacea had been subjected to detailed genetic and mechanistic studies. to determine if the complex structures, termed tailocins, derived from phage- ... | 2017 | 28195220 |
| effect of high hydrostatic pressure combined with moderate heat to inactivate pressure-resistant bacteria in water-boiled salted duck. | the objective of this work was to study the effect of high hydrostatic pressure combined with moderate heat to inactivate pressure-resistant bacteria in water-boiled salted duck meat (wbsdm), and to establish suitable procedures to improve the quality of wbsdm. the conditions (300 mpa/60 °c, 400 mpa/60 °c, and 500 mpa/50 °c) effectively inactivated the pressure-resistant bacteria (bacillus cereus and staphylococcus warneri) in wbsdm. although more pressure-resistant than s. warneri, the above tr ... | 2015 | 25943207 |
| two-stage phyto-microremediation of tannery effluent by spirodela polyrrhiza (l.) schleid. and chromium resistant bacteria. | two-stage sequential treatment of tannery effluent was conducted employing a wetland plant, spirodela polyrrhiza (l.) schleid., and chromium (cr) resistant bacterial strains. the bacterial strains were isolated from cr-enriched environmental matrices and rhizosphere of spirodela polyrrhiza. the phyto-rhizoremediation of tannery effluent by spirodela and its rhizospheric bacteria (cellulomonas biazotea apbr1-6, bacillus safensis apbr2-12, staphylococcus warneri apbr3-5, microbacterium oleivorans ... | 2016 | 27318662 |
| efficient pahs biodegradation by a bacterial consortium at flask and bioreactor scale. | in this work, the biodegradation of three polycyclic aromatic hydrocarbons (pahs) such as phenanthrene (phe), pyrene (pyr) and benzo[a]anthracene (baa) has been investigated. a bacterial consortium consisting of two strains was used for the first time based on preliminary promising biodegradation data. they were tentatively identified as staphylococcus warneri and bacillus pumilus. degradation values higher than 85% were obtained for each single pah when operating at flask scale, whereas minimum ... | 2012 | 22738812 |
| viability of phenanthrene biodegradation by an isolated bacterial consortium: optimization and scale-up. | in the present work, biodegradation of phenanthrene by a bacterial consortium (lb2), isolated from lab-polluted soils has been investigated. the 16s rrna gene-based molecular analysis revealed that the bacterial consortium lb2 consisted of two strains showing a very high homology with staphylococcus warneri and bacillus pumilus. the optimization of phenanthrene degradation by the consortium lb2, using a central composite face-centered design was carried out taking into account three important pa ... | 2013 | 22717666 |
| direct detection of burkholderia cepacia in susceptible pharmaceutical products using semi-nested pcr. | burkholderia cepaciahas recently received a considerable attention as one of the major risks in susceptible pharmaceutical products. this microorganism can easily propagate and cause vast and severe contamination, especially to the water supplies for pharmaceutical companies. moreover, it proliferates within the products and can cause severe infections for humans. therefore, fast and sensitive detection of these bacteria is of a great demand. the present study introduces improved application of ... | 2016 | 26797972 |
| microbiota at teeth and implants in partially edentulous patients. a 10-year retrospective study. | to determine the microbiota at implants and adjacent teeth 10 years after placement of implants with a sandblasted and acid-etched surface. | 2016 | 25827437 |
| microbial diversity in persistent root canal infections investigated by checkerboard dna-dna hybridization. | the aim of the present study was to investigate the composition of the root canal microbiota in endodontic failures in order to identify and quantify these microorganisms. | 2014 | 24935532 |
| [pathogens of prostatitis and their drug resistance: an epidemiological survey]. | to investigate the epidemiological features of the pathogens responsible for prostatitis in the changshu area, and offer some evidence for the clinical treatment of prostatitis. | 2013 | 24218946 |
| 5α-androst-16-en-3α-ol β-d-glucuronide, precursor of 5α-androst-16-en-3α-ol in human sweat. | 5α-androst-16-en-3α-ol (α-androstenol) is an important contributor to human axilla sweat odor. it is assumed that α-andostenol is excreted from the apocrine glands via a h2 o-soluble conjugate, and this precursor was formally characterized in this study for the first time in human sweat. the possible h2 o-soluble precursors, sulfate and glucuronide derivatives, were synthesized as analytical standards, i.e., α-androstenol, β-androstenol sulfates, 5α-androsta-5,16-dien-3β-ol (β-androstadienol) su ... | 2013 | 24327440 |
| design a cada-targeted dna probe for screening of potential bacterial cadmium biosorbents. | due to their metal removal ability, bacterial biosorbents can be effectively used for the treatment of wastewaters containing heavy metals. searching for bacterial biosorbents for hazardous heavy metals like cadmium is a pivotal for remediation efforts. the gene cada, that mediates resistance to cadmium over an atp-dependent efflux mechanism, provides a good target for the selection of potential cadmium biosorbents. for this reason, in this study, a 36-mer-oligonucleotide dna probe based on the ... | 2016 | 26585451 |
| rainbow trout (oncorhynchus mykiss) secretory component binds to commensal bacteria and pathogens. | commensal bacteria co-exist on the mucosal surfaces of all vertebrates. the host's mucosal immune system must tolerate commensals while fighting pathogens. one of the mechanisms used by the mucosal immune system to maintain homeostasis is the secretion of immunoglobulins (igs) across epithelial barriers, which is achieved via the polymeric immunoglobulin receptor (pigr). rainbow trout pigr is known to transport igt and igm across epithelia. however, other biological functions for trout pigr or t ... | 2017 | 28150752 |
| microbiological diversity associated with the spontaneous wet method of coffee fermentation. | the evaluation of the microbiota present during coffee wet fermentation was done in two distinct regions of minas gerais, brazil: one farm in the south of minas gerais (lavras=l) and another farm in the savannah region (monte carmelo=mc). the yeast population ranged from 2.48 to 4.92 log cfu/g and from 2 to 4.81 log cfu/g, the mesophilic bacteria population ranged from 3.83 to 8.47 log cfu/g and from 5.37 to 7.36 log cfu/g, and the lab population ranged from 2.57 to 5.66 log cfu/g and from 3.40 ... | 2015 | 26119187 |
| overall biochemical changes in bacteria photosensitized with cationic porphyrins monitored by infrared spectroscopy. | photodynamic inactivation of micro-organisms is a promising nonantibiotic multitarget approach to treat localized and superficial infections through oxidative stress. herein, the changes occurring on major cellular components of escherichia coli and staphylococcus warneri, induced by photosensitization with cationic porphyrins (tri-py(+)-me-pf and tetra-py(+)-me) and white light, were monitored by infrared spectroscopy. | 2016 | 27073984 |
| rapid and fully automated bacterial pathogen detection on a centrifugal-microfluidic labdisk using highly sensitive nested pcr with integrated sample preparation. | diagnosis of infectious diseases suffers from long turnaround times for gold standard culture-based identification of bacterial pathogens, therefore impeding timely specific antimicrobial treatment based on laboratory evidence. rapid molecular diagnostics-based technologies enable detection of microorganisms within hours however cumbersome workflows and complex equipment still prevent their widespread use in the routine clinical microbiology setting. we developed a centrifugal-microfluidic "labd ... | 2015 | 26235430 |
| protein profiles of escherichia coli and staphylococcus warneri are altered by photosensitization with cationic porphyrins. | oxidative stress induced by photodynamic treatment of microbial cells causes irreversible damages to vital cellular components such as proteins. photodynamic inactivation (pdi) of bacteria, a promising therapeutic approach for the treatment of superficial and localized skin and oral infections, can be achieved by exciting a photosensitizing agent with visible light in an oxygenated environment. although some studies have addressed the oxidative alterations of pdi in bacterial proteins, the prese ... | 2015 | 25959378 |
| infectious spondylodiscitis - a case series analysis. | we aimed to describe the clinical and laboratory features as well as diagnostic difficulties in the case series of spondylodiscitis. | 2014 | 24797976 |
| antibacterial activity of essential oils, their blends and mixtures of their main constituents against some strains supporting livestock mastitis. | ten of the most known and used commercial essential oils (cinnamomum zeylanicum l., citrus bergamia risso, eucalyptus globulus labill., foeniculum vulgare mill., origanum majorana l., origanum vulgare l., rosmarinus officinalis l., satureja montana l., thymus vulgaris l. ct. carvacrol, thymus vulgaris l. ct. thymol) were tested against six bacteria strains staphylococcus aureus, staphylococcus chromogenes, staphylococcus sciuri, staphylococcus warneri, staphylococcus xylosus and escherichia coli ... | 2014 | 24727086 |
| nucleic acid changes during photodynamic inactivation of bacteria by cationic porphyrins. | light activation of photosensitizing dyes in presence of molecular oxygen generates highly cytotoxic reactive oxygen species leading to cell inactivation. nucleic acids are molecular targets of this photodynamic action but not considered the main cause of cell death. the in vivo effect of the photodynamic process on the intracellular nucleic acid content of escherichia coli and staphylococcus warneri was evaluated herein. two cationic porphyrins (tetra-py(+)-me and tri-py(+)-me-pf) were used to ... | 2013 | 23719285 |
| purification and antibacterial activity of recombinant warnericin rk expressed in escherichia coli. | warnericin rk is a small cationic peptide produced by staphylococcus warneri rk. this peptide has an antimicrobial spectrum of activity almost restricted to the legionella genus. it is a membrane-active peptide with a proposed detergent-like mechanism of action at high concentration. moreover, the fatty acids content of legionella was shown to modulate the peptide activity. in order to decipher the mode of action in details using solid-state nmr spectroscopy, large amount of an isotopic labeled ... | 2013 | 23053077 |
| microbiological contamination of drugs during their administration for anesthesia in the operating room. | the aseptic techniques of anesthesiologists in the preparation and administration of injected medications have not been extensively investigated, but emerging data demonstrate that inadvertent lapses in aseptic technique may be an important contributor to surgical site and other postoperative infections. | 2016 | 26845141 |
| analysis of microbiota on abalone (haliotis discus hannai) in south korea for improved product management. | abalone is a popular seafood in south korea; however, because it contains various microorganisms, its ingestion can cause food poisoning. therefore, analysis of the microbiota on abalone can improve understanding of outbreaks and causes of food poisoning and help to better manage seafood products. in this study, we collected a total of 40 abalones from four different regions in march and july, which are known as the maximum abalone production areas in korea. the microbiota were analyzed using hi ... | 2016 | 27371902 |
| two-component systems involved in susceptibility to nisin a in streptococcus pyogenes. | two-component systems (tcss) are regulatory systems in bacteria that play important roles in sensing and adapting to the environment. in this study, we systematically evaluated the roles of tcss in the susceptibility of the group a streptococcus (gas; streptococcus pyogenes) sf370 strain to several types of lantibiotics. using individual tcs deletion mutants, we found that the deletion of srtrk (spy_1081-spy_1082) in sf370 increased the susceptibility to nisin a, which is produced by lactococcus ... | 2016 | 27474716 |
| cefotaxime prevents microbial contamination and improves microspore embryogenesis in wheat and triticale. | cefotaxime (100 mg/l) mitigate occasional gram negative bacterial contamination in wheat and triticale microspore culture and most importantly it increases cell growth and green plant production. isolated microspore culture is a promising option to rapidly fix the product of meiotic recombination of f1 hybrids, in the process of varietal development. clean culture and high embryogenesis rate are essential to commercial triticale and wheat microspore cultures. so, this study investigated (1) cont ... | 2013 | 23896731 |
| bioprospecting from cultivable bacterial communities of marine sediment and invertebrates from the underexplored ubatuba region of brazil. | shrimp fisheries along the brazilian coast have significant environmental impact due to high by-catch rates (21 kg per kilogram of shrimp). typically discarded, by-catch contains many invertebrates that may host a great variety of bacterial genera, some of which may produce bioactive natural products with biotechnological applications. therefore, to utilize by-catch that is usually discarded we explored the biotechnological potential of culturable bacteria of two abundant by-catch invertebrate s ... | 2017 | 27644133 |