| modification of the immunologic properties of the cell surface. | living cf1 mouse-transplantable spontaneous mammary adenocarcinoma cells were modified with glutaraldehyde, formalin, 2,4,6-trinitrophenylate, vibrio cholerae neurominidase, iodoacetate, heparin, histamine and adenosine 3'5'-monophosphate (camp), then used to immunize syngeneic cf1 mice. animals immunized with the fixed (formalinized or glutaraldehyde fixed) neuraminidase-treated cells or the membrane of these cells, rejected two challenging doses of 10(8) viable unmodified adenocarcinoma cells. ... | 1978 | 24583 |
| evaluation of the effectiveness of receptor destroying enzyme preparations. | a procedure has been developed for testing receptor destroying enzyme (rde) preparations used to remove nonspecific inhibitors before carrying out haemagglutination inhibition tests with influenza virus. four criteria should be taken into account: (1) titre of rde, usually used to indicate the activity of the preparation; (2) neuraminidase activity of the preparation, determined biochemically, which partially corresponds to the rde titre; (3) direct demonstration of the complete removal of nonsp ... | 1977 | 23666 |
| pathophysiological effects of vibrio cholerae and enterotoxigenic escherichia coli and their exotoxins on eucaryotic cells. | | 1978 | 82199 |
| demonstration of shared and unique immunological determinants in enterotoxins from vibrio cholerae and escherichia coli. | immunodiffusion and biological neutralization studies demonstrated that the heat-labile enterotoxin (lt) from escherichia coli has antigenic determinants in common with each of the isolated subunits (a and b) of the enterotoxin (choleragen) from vibrio cholerae. each of the enterotoxins also possesses unique antigenic specificities. monospecific antiserum to lt was prepared by immunization with antigens derived by immune precipitation of e. coli cell-free supernatant with isolated specific antic ... | 1978 | 83301 |
| pyruvate kinase from the moderate halophile, vibrio costicola. | | 1977 | 19142 |
| the displaced ganglion cell in the avian retina: developmental and comparative considerations. | | 1979 | 84539 |
| modes of transmission of cholera in a newly infected population on an atoll: implications for control measures. | to determine the modes of transmission in a cholera epidemic in a newly infected population on an atoll, two studies were conducted, in which persons with cholera were individually matched, by age and sex, with neighbourhood controls. among the first recognised cases, cholera was associated with frequent (daily or weekly) consumption of raw lagoon fish that had been salted and partially dried. vibrio cholerae was isolated from the lagoon, which was contaminated by human wastes. during the declin ... | 1979 | 84960 |
| rapid emergence of el tor vibrio cholerae resistant to antimicrobial agents during first six months of fourth cholera epidemic in tanzania. | 110 el tor vibrio cholerae isolates from 102 patients with cholera between november, 1977, and march, 1978, during the early stages of the fourth epidemic of cholera in tanzania had minimum inhibitory concentrations to tetracycline, chloramphenicol, nitrofurantoin, neomycin, ampicillin, and sulphadimidine determined. all isolates during the first month after the disease was recognised were fully sensitive to tetracycline, but 76% of isolates were resistant to the drug after five months of extens ... | 1979 | 85001 |
| leprosy's footprints in bone-marrow histiocytes. | | 1979 | 85259 |
| [resistence of head lice against gammexane (lindane) applies also to the nonalcoholic preparations]. | | 1979 | 85264 |
| purification and properties of a membrane-associated, folate-binding protein from lactobacillus casei. | a folate-binding protein has been solubilized from lactobacillus casei by treatment of membrane preparations with triton x-100 in the presence of 3hfolate. the protein-folate complex was purified 100-fold and recovered in a 22% yield by adsorption and elution from microgranular silica (quso g-32), followed by passage through sephadex g-150. when subjected to sodium dodecyl sulfate/polyacrylamide gel electrophoresis, the purified preparations showed only a single, protein-staining band whose mole ... | 1977 | 16917 |
| in vitro synthesis of infectious transforming dna by the avian sarcoma virus reverse transcriptase. | infectious dna molecules, capable of transforming chicken embryo fibroblasts, can be synthesized by the rous sarcoma virus-associated reverse transcriptase in vitro. the optimal enzymatic conditions employed for infectious dna synthesis also facilitate maximum synthesis of genome length dna. analysis of the dna product synthesized by detergent-disrupted rous sarcoma virus under these conditions indicates that dna complementary to viral rna (minus-strand dna) is genome length in size, whereas dna ... | 1979 | 85719 |
| alpha-isopropylmalate synthase from alcaligenes eutrophus h 16 i. purification and general properties. | alpha-isopropylmalate (ipm) synthase, the first enzyme in the biosynthesis of l-leucine, was purified to a specific activity of 12 micronmole/min x mg protein from the valine-isoleucine double auxotrophic mutant a-81 of the hydrogen bacterium alcaligenes eutrophus h16. the activity in crude extracts of derepressed cells was 0.106 micronmoles of isopropylmalate formed per min and per mg protein. gel electrophoresis and regel electrophoresis of the isolated main band resulted in several distinct b ... | 1977 | 16576 |
| immunochemical properties of oligosaccharide-protein conjugates with klebsiella-k2 specificity. i. specificity and crossreactivity of anti-conjugate versus anti-bacterial antibodies. | | 1979 | 86152 |
| dna polymerase with characteristics of reverse transcriptase purified from human milk. | a dna polymerase purified from a particulate fraction of human milk has biochemical and biophysical properties similar to those of viral reverse transcriptases. this enzyme is immunologically distinct from cellular dna polymerases obtained from a variety of human sources. | 1979 | 86209 |
| light-activated calcium release from sonicated bovine retinal rod outer segment disks. | calcium trapped within sonicated and resealed bovine rod outer segment disks is released upon light exposure with a stoichiometry of 0.75 +/- 0.05 calcium for each rhodopsin bleached. the amount of calcium liberated is proportional to the amount of bleaching in the range of 20 to 100% bleaching and is relatively insensitive to the internal trapped calcium concentration. the results are obtained using a flow system in which the disk membrane vesicles are adsorbed on glass particle supported by a ... | 1977 | 14680 |
| interplexiform cell of the mouse retina: a golgi demonstration. | a neuron of the inner nuclear layer (inl) with some processes extending to the outer plexiform layer (opl) and others to the inner plexiform layer (ipl) was discovered near the posterior pole of the mouse retina. the neuron's location and appearance are similar to the interplexiform cells of several other species. the relatively recent recognition of this cell type along with its characteristic of infrequent staining by the golgi technique make the extent of its distribution among species uncert ... | 1979 | 86528 |
| the puzzle of "urethral syndrome": a possible answer? | 82 strains of slow-growing, co2-dependent, gram-positive organisms have been isolated from the urine of 9 male and 73 female patients. 100% of the men and 93% of the women had urinary symptoms at the time of isolation, and 66% of the specimens showed pyuria. these organisms might account for the urinary symptoms of some patients previously diagnosed as having "urethral syndrome". preliminary data show that, if appropriate antibacterial treatment is given, most of these patients become symptom-fr ... | 1979 | 86778 |
| definition of two ld antigens in rhesus monkeys. | two rhesus (macaca mulatta) monkey lymphocyte-defined (ld) antigens have been identified using two typing cells as stiumlators in a one-way mixed leukocyte culture (mlc) assay. an analysis of the genetic behavior of these ld antigens in six rhesus monkey families revealed that both antigens were linked with rhla. one probable recombinant indicated that the ld locus lies outside the two known rhla-sd loci and the locus which controls the serum protein, properdin b(bf). these two antigens, ld1 and ... | 1979 | 87026 |
| comparative study of the proteinograms of vibrios and of closely related microorganisms by means of polyacrylamide gel disc electrophoresis. | proteinograms of 112 strains of vibrios and closely affiliated microorganisms were studied by disc electrophoresis in polyacrylamide gel. up to 25 protein peaks with definite mobility coefficients were revealed. the influence of the culture medium on the protein spectrum of the microbes was found. the frequency of peak formation was of great significance for the differentiation of the microbes under study. the quantitative characteristics of the peak area could not be used for differentiation. | 1976 | 7908 |
| cytotoxicity by cultured human lymphocytes: characteristics of effector cells and specificity of cytotoxicity. | | 1979 | 87435 |
| the separate roles of hapten and of carrier in the conformation of t cell-reactive determinants on aba-protein conjugates. | | 1979 | 87448 |
| regulation of ige antibody production by serum molecules. v. evidence that coincidental sensitization and imbalance in the normal damping mechanism results in "allergic breakthrough". | experiments presented in this paper were designed to test a new concept concerning the possible pathogenesis of the allergic phenotype. this concept, termed "allergic breakthrough" considers that one of the avenues toward the allergic phenotype involves coincidental sensitization combined with an imbalance in the normal damping mechanism that serves to limit ige antibody production. the three predictions of this concept that can be tested experimentally are: 1) manipulations that are effective i ... | 1979 | 87455 |
| ige antibody formation and aging. i. age-related changes in ige antibody formation and avidity for the dnp-determinant in mice. | | 1979 | 87474 |
| cytotoxic macrophages induced in hamsters by vaccinia virus: selective cytotoxicity for virus-infected targets by macrophages collected late after immunization. | | 1979 | 87476 |
| hour of onset of cholera and diarrhoea. | | 1979 | 87933 |
| the cerebellar projection from the lateral reticular nucleus as studied with retrograde transport of horseradish peroxidase. | the cerebellar projection from the lateral reticular nucleus (nrl) was studied in cats by means of retrograde axonal transport of horseradish peroxidase (the projection to the paramedian lobule was not included, see brodal, 1975, for afferents to this cortical region). the entire cerebellar cortex and all cerebellar nuclei receive fibres from the nrl. the strongest connection is with the anterior lobe and lobulus viiib of the posterior lobe vermis. as concerns the anterior lobe the observations ... | 1979 | 88191 |
| 4th international workshop on human gene mapping. report of the committee on the genetic constitution of the x and y chromosomes. | | 1978 | 88299 |
| lipopolysaccharide-derived serotype polysaccharides from neisseria meningitidis group b. | three immunologically distinct types of polysaccharides have been isolated by diethylaminoethyl (deae) chromatography from the lipopolysaccharide extracts of group b neisseria meningitidis. all types contain a set of common determinants, as well as distinct ones; all of these determinants are detectable by either immunodiffusion or enzyme-linked immunosorbent assay (elisa). the polysaccharides elute from a sepharose 4b column in the range of 2-3 x 10(5) daltons and have isoelectric points from 4 ... | 1979 | 88491 |
| specific binding of prolactin by the prostate gland of the rat and man. | specific binding sites for 125iodine-prolactin are present in membrane particles obtained from the rat ventral prostate, human benign prostatic hyperplasia and prostatic adenocarcinoma. in the ventral prostate glands of young rats (1 to 4 months old) specific binding of 125iodine-prolactin is higher than in older animals (greater than 8 months old). subcellular distribution studies revealed that specific 125iodine-prolactin binding activity is associated primarily with the 15,000 and 100,000 g p ... | 1979 | 88529 |
| techniques for evaluating humoral and cell-mediated immunity in mule deer fawns (odocoileus hemionus). | twenty mule deer fawns (odocoileus hemionus) were removed from their dams 48 h after birth, and hand-reared. methods for monitoring their immune capability are described. passive humoral immunity was determined by serum protein electrophoresis. active humoral immunity following clostridium toxoid vaccination was determined by immunodiffusion. cell-mediated immunity was assayed using contact sensitization to 1-nitro, 2,4-dichlorobenzene (dncb). | 1979 | 88530 |
| resistant vibrio cholerae el tor in tanzania. | | 1979 | 88577 |
| [immunotherapy of malignant growths (author's transl)]. | our knowledge of antigens which are associated with different types of malignant tumours is steadily increasing. these antigens exist in considerable numbers, but, so far with few exceptions, only their presence can be demonstrated by certain methods; to isolate and identify them has not yet been possible. these antigens are, therefore, suitable not so much for the primary diagnosis, but rather, like the carcinoembryonic antigen, the tissue-polypeptide antigen or the alpha-feto-protein, for asse ... | 1979 | 88728 |
| comparative studies of hageman factor (factor xii) in mammalian plasmas by immunological techniques. | | 1979 | 88740 |
| properties of cholera toxin- and naf-stimulated adenylate cyclase from mouse thymocytes. | kinetic parameters of mouse thymocyte adenylate cyclase activity were determined. naf and cholera toxin stimulated adenylate cyclase. stimulation by either agent did not change the ph or mg2+ optima relative to control (unstimulated cyclase). the km value for atp of adenylate cyclase stimulated by naf was significantly reduced from control. by contrast, cholera toxin treatment did not change the km relative to control. adenylate cyclase, when stimulated by naf, had an optimum for mn2+ alone, or ... | 1976 | 3217 |
| studies on histamine release induced by compound 48/80 and peptide 401 [proceedings]. | | 1979 | 88890 |
| differentiation between cyclic amp level and allergic histamine release in mast cells [proceedings]. | | 1979 | 88891 |
| a new assay of estrogen receptor by thin-layer gel filtration. | | 1979 | 88911 |
| alterations in distribution of sudanophilic lesions in rabbits after cessation of a cholesterol-rich diet. | | 1979 | 88945 |
| fetal syphilis in the first trimester. | evidenc of first-trimester fetal syphilis was sought in the products of conception in a therapeutic abortion clinic. during two collection periods of one week, five patients with serologic and clinical data consistent with recent syphilitic infection were identified. their conceptuses were carefully examined by silver and immunofluorescent stains for the presence of treponema pallidum. two of these five conceptuses (9 and 10 weeks' gestation) were found to contain t. pallidum by these methods. t ... | 1976 | 56895 |
| the value of alpha-fetoprotein and beta-human chorionic gonadotrophin in the management of testicular tumours. | serum alpha-fetoprotein and beta-human chorionic gonadotrophin levels were estimated in 115 patients. sixty-nine patients had non-seminomatous germ cell tumours and 34 patients had seminomas. twenty-six patients showed elevated serum makers. | 1978 | 88986 |
| differential mechanisms of increased alpha 1-fetoprotein production in rats following carbon tetrachloride injury and partial hepatectomy. | possible differences in the mechanisms of increased alpha1-fetoprotein (afp) production following carbon tetrachloride (ccl4) intoxication and partial hepatectomy were studied with 5-week-old rats at the time of sacrifice. the maximum level of serum afp reached in 4 days after a single dose of ccl4 was much higher than that after partial hepatectomy, although the incorporation of [3h]thymidine into liver dna increased nearly to the same extent by either of these treatments. in the remnant after ... | 1976 | 58715 |
| trasylol prevents trypsin-induced shock in dogs. | the effect of simultaneous intravenous administration in the dog of bovine trypsin and trasylol followed by continued infusion of trasylol was studied. special attention was paid to the interchange between the dominating plasma protease inhibitors alpha1-antitrypsin and a-macroglobulins and to the disappearance of trasylol and its trypsin complexes from the circulation. the following results were obtained: 1) trypsin was preferentially bound by the alpha-macroglobulins, though trasylol is a stro ... | 1979 | 89067 |
| complement-induced histamine release from human basophils. iii. effect of pharmacologic agents. | human serum activated with zymosan generates a factor (c5a) that releases histamine from autologous basophils. previously we have presented evidence that this mechanism for c5a-induced release differs from ige-mediated reactions. the effect of several pharmacologic agents known to alter ige-mediated release was studied to determine whether they have a similar action on serum-induced release. deuterium oxide (d2o), which enhances allergic release, inhibited in a concentration-dependent fashion th ... | 1977 | 65434 |
| [application to the study of connections in the cns of the retrograde axonal transport of an iron-dextran complex]. | the retrograde axonal transport of an iron-dextran complex was observed in neurons of the substantia nigra and of the intralaminar nuclei of the thalamus, after previous injection into the striatum. the histochemical demonstration of iron is simple and rapid, and can be combined with that of horseradish peroxidase, under precise conditions in the sequence of reactions. the iron-dextran complex revealed to be a valuable material for neuronal connectivity studies in the central nervous system. | 1979 | 89914 |
| antigenic drift in type a influenza virus: sequence differences in the hemagglutinin of hong kong (h3n2) variants selected with monoclonal hybridoma antibodies. | | 1979 | 90425 |
| [methodical recommmendations for conduction of the hemagglutination test with erythrocytic cholera enterotoxic diagnostic agent (eceda)]. | | 1976 | 66381 |
| relative affinity of antisera for myelin basic protein (mbp) and degree of affinity heterogeneity. | | 1979 | 90641 |
| [use of color differential medium and russell-i medium in the diagnosis of cholera vibrio]. | | 1977 | 67254 |
| the genetics of an expanded secretor system. | the genetics of salivary secretion of abh blood group substance was investigated. eight secretor phenotypes were determined by sephadex g-100 chromatography. presence or absence of three different fractions were shown to be controlled by three different secretor gene loci. these loci are called sec1, sec2 and sec3. sec1 controls expression of the traditional secretor status. sec2 and sec3 are linked, but distinct, loci controlling the expression of low molecular weight abh substance in human sal ... | 1979 | 90711 |
| specific inhibition of viral neuraminidases by an inhibitor, neuraminin, produced by streptomyces sp. | | 1977 | 67704 |
| bottle feeding as a risk factor for cholera in infants. | to determine risk factors for cholera in infants, a retrospective matched-pair study of 42 cases and their controls was undertaken during an outbreak of el tor cholera in bahrain in the autumn of 1978. the highest attack-rate of cholera (125/10 000) occurred in infants in the 6--11 month age-group, which corresponds to the weaning age in this community. significantly more cases than controls were principally bottle fed (greater than 50% milk intake by bottle) than principally breast fed during t ... | 1979 | 90813 |
| antimicrobial susceptibility of vibrio cholerae from zaire and rwanda. | | 1979 | 90940 |
| viral liver disease. | | 1979 | 91032 |
| the structure and evolution of the hla--bw4 and bw6 antigens. | the hla--bw4 and bw6 antigenic determinants have been shown to co-migrate with hla--b determinants on gel-filtration, sucrose density gradient centrifugation and affinity chromatography, using lens culinaris lectin and antibody against human beta 2 microbulin. these and other published data imply that the hla--bw4 and bw6 determinants reside on the same polypeptide chain as other hla--b locus determinants. the implications of this in terms of the evolution of cross reacting groups of antigens at ... | 1979 | 91209 |
| [use of antistaphylococcal immune preparations in acute suppurative-septic staphylococcal diseases]. | | 1979 | 91270 |
| latex fetuin spheres as probes for influenza virus neuraminidase in productively and abortively infected cells. | fetuin bound latex spheres do not adhere to the membranes of non-infected cells but adhere to those of cells productively infected by fowl plague virus (fpv dobson strain). in contrast, asialo fetuin spheres do not attach to the membranes of productively infected cells. moreover latex fetuin spheres incubated with extracts of productively infected cells and extensively washed are specifically enriched in neuraminidase activity without any trace of haemagglutinin. these observations suggest that ... | 1979 | 91354 |
| experimental cancer immunotherapy: modification of tumor cells to increase immunogenicity. | firmly established transplantable c3h/hej mammary carcinomas can be inhibited by host challenge with vibrio cholerae neuraminidase (vcn)-treated tumor cells. the effect is totally immunospecific, even vcn-treated tumors bearing shared mammary tumor virus (mtv) antigen cannot induce the regression. thus, vcn is capable of increasing the immunogenicity of the private, unique-unshared tumor antigens on mammary carcinomas; vcn is incapable of increasing the immunogenicity of the shared mtv-associate ... | 1976 | 68699 |
| [anti-arrhythmic effect of tetrodotoxin in the late stage of experimental myocardial infarct]. | the action of tetrodotoxin (tt), blocking the fast sodium current, on arrhythmias that occurred 24 hours after occlusion of the coronary artery was studied in 10 dogs. tt injected intravenously in doses of 0.5--3.0 micrograms/kg significantly decreased the number of ventricular extrasystoles, and completely restored sinum rhythm in 4 animals. the maximum antiarrhythmic effect was noted 3 to 5 minutes after tt adminstration. it is suggested that arrhythmias and the antiarrhythmic effect of the dr ... | 1979 | 91393 |
| characterization of the thyrotropin receptor and its involvement in graves' disease. | | 1977 | 69601 |
| [possible errors in bacteriological diagnosis of cholera]. | | 1977 | 69759 |
| procaine-induced seizures in epileptic monkeys with bilateral hippocampal foci. | intravenous procaine hcl given at low doses (0.5-2.5 mg/kg) to two monkeys with bilateral alumina hippocampal foci depressed interictal spiking or had little effect. at 5.0 mg/kg unilateral limbic activation occurred. at 10.0 mg/kg unilateral or bilateral limbic activation and generalized seizures could be evoked within 3-10 min. at higher doses (15 and 20 mg/kg) bilateral limbic activation or brief (one min) generalized seizures occurred. the unilateral-onset psychomotor seizures were not ident ... | 1979 | 91502 |
| antigenic correlation between rat brain synaptic vesicles and rat bone marrow b lymphocytes. | rabbit anti-rat brain synaptic vesicle serum reacted with thymocytes and b lymphocytes in cytotoxicity and immunofluorescence assays. quantitative absorption analysis revealed that this antiserum contained antibodies specific for antigenic determinants on the surface membrane of a subpopulation of rat bone marrow b lymphocytes. | 1979 | 91533 |
| generation of a fibroblast chemotactic factor in serum by activation of complement. | when serum complement is activated by either the classical or alternative pathways, a factor with an apparent 80,000 mol wt is generated that is chemotactic for human dermal fibroblasts. the origin of this serum-derived chemotactic factor (sdcf) is not known; however, it may be a cleavage product from c5 because it is inactivated by monospecific antiserum to human c5, and it is not generated when the complement system is activated in human serum deficient in c5. sdcf is not chemotactic for human ... | 1979 | 91624 |
| human lung tumor-associated antigens of 32,000 daltons molecular weight. | lung tumor-associated antigens of approximately 32,000 daltons were recognized by the use of sensitive radioimmunoassays and rabbit antisera, one raised against an extract of pooled human malignant lung tissues and another raised against a cell line derived from a human squamous cell carcinoma of the lung. these antigens differ from antigens described previously, including carcinoembryonic antigen and alpha-fetoprotein. the antigens were detected on 13 of 13 lung tumors (of all histologic types) ... | 1979 | 91695 |
| [dry media on yeast hydrolysates for growing vibrio cholerae]. | | 1977 | 72881 |
| secretory iga against enterotoxins in breast-milk. | a pool of colostrum from guatemalan mothers (guatemalan colostrum)) obtained 2-4 days post partum inhibited the induced fluid accumulation in rabbit ileal loops when incubated with vibrio cholerae or escherichia coli enterotoxin. there was a linear relationship between the quantity of colostrum used and the protection achieved. pools of guatemalan breast-milk obtained 15-30 days post partum and north american breast-milk had the same effect when tested with e. coli and v. cholerae enterotoxins, ... | 1976 | 73692 |
| micromplement fixation studies of human plasma prothrombin and purified prothrombin preparations. | | 1979 | 92071 |
| [cutaneous leishmaniasis. alcoy: an endemic zone]. | | 1979 | 92178 |
| horizontal organization of velocity characteristics in area 18 of the cat [proceedings]. | | 1979 | 92238 |
| effect of fat structure on total, on chylomicron, and on high-density lipoprotein cholesterol in the absorptive state in man [proceedings]. | | 1979 | 92248 |
| triiodothyronine (t3) receptors in brain and liver during induced hypothyroidism in rats [proceedings]. | | 1979 | 92254 |
| fate of a "maturation-specific" messenger rna during early germination of mung-bean seeds [proceedings]. | | 1979 | 92270 |
| [experimental use of prostaglandin f2 alpha to synchronize estrus in cattle]. | experiments were conducted for the use of prostaglandin to synchronise oestrus in dairy cows. one single dose of 5 mg f2-alpha prostaglandin was administered via the intrauterine route. the reference values of the control groups were matched by first insemination following induced oestrus among the best of the test animals which were inseminated twice. these values reached 72 and 96 hours following application accounted for 46.7 and 50.0 per cent. the yellow body phase, a prerequisite for oestru ... | 1979 | 92295 |
| immunological cross-reactivity between acid extracts of myelin, liver and neoplastic tissues: studies in immunized guinea-pigs. | groups of 4 guinea-pigs were immunized with acid extracts prepared from bovine myelin (ef), normal human liver tissue and malignant or benign neoplastic tissues in freund's complete adjuvant (fca1. the animals were weighed daily and examined for clinical signs of experimental allergic encephalomyelitis (eae). all the animals immunized with ef developed clinical symptoms of eae within 21 days of the initial immunization, whilst some of the animals immunized with certain tumour extracts developed ... | 1979 | 92328 |
| suramin: a potent inhibitor of the reverse transcriptase of rna tumor viruses. | suramin--a well-known antitrypanosomal agent--was found to exert a strong inhibitory effect on the rna-directed dna polymerase (reverse transcriptase) activity of several oncornaviruses such as moloney murine leukemia virus, murine rauscher leukemia viruses, moloney murine sarcoma virus and avian myeloblastosis virus. inhibition of enzyme activity was obtained with both endogenous viral rna and (a)n . oligo(dt) as the template-primer. suramin effected a 50% inhibition of the reverse transcriptas ... | 1979 | 92362 |
| phage-induced changes in vibrio cholerae: serotype and biotype conversions. | phage infection of vibrio cholerae resulted in antigenic changes. a strain of biotype cholerae serotype ogawa was converted into serotype hikojima and gained the ability to synthesize antigenic factor c. some phage-converted strains remained stable after subculture and were immune to superinfection with the same phage. the stable converted strains were lysogenic and released phage having a host range similar to the phage of the donor strain. reinfection of unstable converted strains which had "l ... | 1978 | 75188 |
| antigenic relationship between the common antigen (oep) of pseudomonas aeruginosa and vibrio cholerae. | antibodies were found by the oep-passive hemagglutination test to cross-react with the common antigen (oep) of pseudomonas aeruginosa in sera of rabbits immunized with two serotype (inaba and ogawa) strains of vibrio cholerae. the titer in the oep-passive hemagglutination reaction rose later than did the agglutinin titer and reached a peak of 640 to 1,280. the titers of oep antibody formation in rabbits immunized with v. cholerae were almost the same as that of p. aeruginosa. the common antigen ... | 1978 | 75846 |
| immunoelectron microscopic localization of herpes simplex virus antigens in infected cells using the unlabeled antibody-enzyme method. | subcellular localization of viral antigens was demonstrated during viral morphogenesis using herpes simplex virus type 1 (hsv-1) infected monolayers of rabbit cornea cells. the localization was done by immunoelectron microscopy employing the peroxidase-antiperoxidase (pap) immunocytochemical technique and the postembedding staining method. the localization of viral antigens was followed at time intervals during infection from 2 to 19 hr. after exposure of sections to either polyspecific antibodi ... | 1979 | 92500 |
| evaluation of the validity of the histochemical lead nitrate technique for alkaline and acid deoxyribonuclease. | the results of several tests and the characteristic morphological distribution of the enzymatic activity appeared to be in favor of the validity and specificity of the histochemical lead nitrate technique for alkaline and acid deoxyribonuclease (dnase) detection. these tests included thermal inhibition, omission of substrate, use of different chemical inhibitors and the reproduction of histochemical staining on coujard's slides. most of these results were in conformity with the biochemical data ... | 1979 | 92502 |
| [use of dry nutrient media for identification of the vibrio]. | | 1978 | 75999 |
| ia determinants on human t-cell subsets defined by monoclonal antibody. activation stimuli required for expression. | the nature of ia antigens which appear on human t cells after activation and the stimuli required for their expression was examined utilizing a monoclonal antibody reactive with the ia antigen framework. t cells were purified using monoclonal antibodies directed either at the entire t-cell population (okt3) or the t-cell inducer subset (okt4). by indirect immunofluorescence, it was shown that the human t-cell population contains no detectable ia+ cells in the resting state. in contrast, in exces ... | 1979 | 92523 |
| a rapid assay for neuraminidase. the detection of two differences in activity associated with virus transformation. | neuraminidase (acylneuraminyl hydrolase, ec 3.2.1.18) activity in fibroblast homogenates was measured by a rapid and simple assay with a synthetic substrate. the activity of neuraminidase in virus transformed hamster fibroblasts was increased over the normal counterpart. in addition, the differential activity seen using the synthetic substrate and fetuin made it possible to detect an enzyme activity hitherto not described. the advantages of this assay for metabolic screening are discussed. | 1978 | 77684 |
| stimulation of cyclic amp secretion in vero cells by enterotoxins of escherichia coli and vibrio cholerae. | the morphological response of vero cells to escherichia coli heat-labile enterotoxin was similar to that of cholera toxin and was accompanied by increases in the intracellular level of cyclic amp. the effects of both enterotoxins were enhanced by the presence of phosphodiesterase inhibitor and inhibited by heat or specific antisera. accumulation of cyclic amp preceded the morphological response. | 1978 | 80381 |
| the accessibility of antigenic determinants of ribosomal protein s4 in situ. | antibodies to escherichia coli ribosomal protein s4 react with s4 in subribosomal particles, eg, the complex of 16s rna with s4, s7, s8, s15, s16, s17, and s19 and the ri reconstitution intermediate, but they do not react with intact 30s subunits. antibodies were isolated by three different methods from antisera obtained during the immunization of eight rabbits. some of these antibody preparations, which contained contaminant antibodies directed against other ribosomal proteins, reacted with sub ... | 1979 | 92615 |
| ultrastructure and partial characterization of a regular array in the cell wall of lactobacillus brevis. | the cell wall of lactobacillus brevis was revealed by electron microscopy to have an outer layer composed of a regular array. the morphological unit of the regular array appeared to consist of four spherical subunits, each about 2 nm in diameter, which were arranged in a tetragonal pattern about 4.5 by 7.0 nm in dimension. the regular array was composed of the tetragonal units in rows in two directions at an angle of about 75 degrees to each other. the average spacing between the rows was about ... | 1979 | 92747 |
| olivocerebellar projections in the cat studied by means of anterograde axonal transport of labelled amino acids as tracers. | | 1979 | 92769 |
| cholera, non-vibrio cholera, and stomach acid. | fasting and postprandial stomach acid production were low in 16 of 37 bangalees convalescing from cholera or non-vibrio cholera. gastric juice of hypochlorhydric patients did not kill cholera vibrios in vitro, whereas that from normochlorhydric patients rapidly killed vibrios in concentrations up to 10(10)/ml. to determine whether hypoacidity resulted from cholera or was a common predisposing factor, basal and betazole-hydrochloride-stimulated acid production were measured before and after chole ... | 1978 | 81410 |
| alpha-feto-protein (afp) and human chorionic gonadotropin (hcg) as biochemical markers of intracranial germ-cell tumours. | | 1979 | 92878 |
| prostate-specific acid phosphatase: purification and specific antibody production in rabbits. | demonstration of prostate-specific acid phosphatase by immunologic methods in tissue sections or in plasma necessitates a monospecific antiserum. this is produced by immunizing rabbits with pure prostate-specific acid phosphatase antigen, prepared from seminal fluid. the ejaculate is centrifuged, dialyzed against a citrate buffer, ph 4.8, and centrifuged again. the supernatant is brought onto a sephacryl s-200 superfine column and eluted with the same buffer. prostate-specific acid phosphatase-p ... | 1979 | 92890 |
| [relations between oxoglutarate transport function and variations of space in rat heart mitochondria (proceedings)]. | | 1979 | 92958 |
| antigenic similarity of morphologic type ciii1 bacteriophages. | the aim of the study was to demonstrate antigenic differentiation among bacteriophages belonging to the same morphologic type--ciii1 according to krzywy and slopek or a2 according to ackermann. twenty-six bacteriophages which multiplied on various strains of bacteria of the genera escherichia, shigella and klebsiella, were studied. serologic tests were done by the quantitative complement fixation test. immune sera against 5 phi e. coli. d8 sh. flexneri, g35 sh. sonnei and kl7 klebsiella bacterio ... | 1979 | 92976 |
| cannabis, hypochlorhydria, and cholera. | in 90 volunteers participating in a vaccine-development programme consumption of beer more than 3 days a week was linked with high stomach acid output, and smoking of cannabis greater than 2 days a week was linked with low acid output. in 92 volunteers challenged with vibrio cholerae or enterotoxigenic escherichia coli, heavy use of cannabis was associated with more voluminous diarrhoea. cannabis use may be an important factor predisposing to severe diarrhoea. | 1978 | 81411 |
| vibrio cholerae in kent. | | 1979 | 84280 |
| the effect of dose loading and of double labelling with 57co and 125i on the tissue distribution in animals. | dose loading effects upon the performance of 57co-bleomycin as a tumor localizing agent have been investigated in rhabdomyosarcoma bearing wag/ry rats. the addition of non-radioactively labelled co-bleomycin increased the relative uptake of 57co-bleomycin in rapid growing tumors, but the addition of non-chelated bleomycin had no influence at all. in our experimental system, iodinated bleomycin generally labelled by reaction with icl, was found to be an unsatisfactory tumor localizing agent. in o ... | 1979 | 93048 |
| [hygienic standards for a mixture of hexachlorane and phosalone in the atmosphere]. | | 1979 | 93066 |
| [antigenic study of "serratia marcescens" isolated in france.ii.--characterization of o antigens and description of 5 new o factors, occurrence of serotypes and labelling of new h factors (author's transl)]. | the somatic o-antigens of 583 french isolates of serratia marcescens were studied with the aid of the tube o-agglutination technique sensitized by centrifugation. flagella antigens were identified by the h-immobilization test. five new o-antigens (o16 to o20) were found. an antigenic factor (co) common to isolates of serotypes o12:h9, o14:h12, o14:h20, and o13:h17 is described. agar cultures of serotypes o12:h9 and o14:h12 dissociated into two colonial types: iridescent colonies, and non-iridesc ... | 1978 | 85425 |
| analysis of vibrio parahaemolyticus soluble antigens by employing passive hemagglutination. | the passive hemagglutination assay was explored as a sensitive test of immunological reactivity of endotoxin and other antigens prepared from selected strains of vibrio parahaemolyticus. the tannic acid procedure for passive hemagglutination, commonly used with protein antigens, was the only procedure yielding good results with v. parahaemolyticus protein extracts, endotoxins, and related preparations. these results were probably due to the presence of large amounts of protein in the v. parahaem ... | 1979 | 93111 |
| experimental infection with ancylostoma caninum larvae in mice. vi. serum protein pattern use due to repeated exposures to infection. | three mice groups were infected with various repeated doses and other four groups with single doses of ancylostoma caninum larvae. serum samples collected at various intervals were analysed electrophoretically. the results were compared first with an uninfected control group and then with those of the corresponding non-immunized single-dose-infected groups. remarkably decreased values of albumin, increased beta globulin and increased total globulins with a decreased a/g ratio were observed. ther ... | 1979 | 93128 |
| memory. | | 1979 | 85894 |