| expression and characterization of polyketide synthase module involved in the late step of cephabacin biosynthesis from lysobacter lactamgenus. | the cephabacins produced by lysobacter lactamgenus are beta-lactam antibiotics composed of a cephem nucleus, an acetate residue, and an oligopeptide side chain. in order to understand the precise implication of the polyketide synthase (pks) module in the biosynthesis of cephabacin, the genes for its core domains, beta-ketoacyl synthase (ks), acyltransferase (at), and acyl carrier protein (acp), were amplified and cloned into the pet-32b(+) expression vector. the sfp gene encoding a protein that ... | 2008 | 18388458 |
| extracellular yeast-lytic enzyme of the bacterium lysobacter sp. xl 1. | an enzyme exhibiting yeast-lytic activity has been isolated from the culture liquid of the bacterium lysobacter sp. xl 1. the optimal conditions for the hydrolysis of saccharomyces cerevisiae cells by the enzyme have been established: 0.15 m sodium acetate buffer, ph 6.0, 50 degrees c. the yeast-lytic activity of the enzyme is inhibited by edta, p-chloromercuribenzoate, and phenylmethylsulfonyl fluoride. according to the data of sds-page, the molecular weight of the protein is 36 kd. the enzyme ... | 2008 | 18393767 |
| secretion of bacteriolytic endopeptidase l5 of lysobacter sp. xl1 into the medium by means of outer membrane vesicles. | the gram-negative bacterium lysobacter sp. xl1 secretes various proteins, including bacteriolytic enzymes (l1-l5), into the culture medium. these proteins are able to degrade gram-positive bacteria. the mechanism of secretion of extracellular proteins by lysobacter sp. xl1 has not been studied hitherto. electron microscopic investigations revealed the phenomenon of the formation of extracellular vesicles by lysobacter sp. xl1. these vesicles contained components of the lysobacter sp. xl1 outer m ... | 2008 | 18573103 |
| oil-removal enhancement in media with keratinous or chitinous wastes by hydrocarbon-degrading bacteria isolated from oil-polluted soils. | the aim of this work was to isolate oil-degrading bacteria that use chitin or keratin as carbon sources from oil contaminated soils; and additionally to study if oil removal by these bacteria is enhanced when a chitinous or a keratinous waste is added to the culture media. to isolate the above-mentioned bacteria, 12 soil samples were collected close to an oil-well. such soils showed unsuitable nutrients content, but their counts of heterotrophic bacteria ranged within 10(5)-10(8) cfu g(-1) soil, ... | 2008 | 18613616 |
| lysobacter ginsengisoli sp. nov., a novel species isolated from soil in pocheon province, south korea. | a gram-negative, aerobic, rod-shaped, nonspore-forming bacterial strain, designated gsoil 357t, was isolated from soil sample of a ginseng field in pocheon province (south korea). the isolate contained q-8 as the predominant ubiquinone and iso-c16:0, iso-c17:1 9c, and iso-c15:0 as the major fatty acids. the g+c content of the genomic dna was 69.3mol%. a phylogenetic analysis based on 16s rrna gene sequences revealed that strain gsoil 357t was most closely related to lysobacter gummosus (97.6%) a ... | 2008 | 18852503 |
| mutagenesis of beta-1,3-glucanase genes in lysobacter enzymogenes strain c3 results in reduced biological control activity toward bipolaris leaf spot of tall fescue and pythium damping-off of sugar beet. | abstract lysobacter enzymogenes produces extracellular lytic enzymes capable of degrading the cell walls of fungi and oomycetes. many of these enzymes, including beta-1,3-glucanases, are thought to contribute to the biological control activity expressed by several strains of the species. l. enzymogenes strain c3 produces multiple extracellular beta-1,3-glucanases encoded by the glua, glub, and gluc genes. analysis of the genes indicates they are homologous to previously characterized genes in th ... | 2005 | 18943787 |
| induced resistance as a mechanism of biological control by lysobacter enzymogenes strain c3. | abstract induced resistance was found to be a mechanism for biological control of leaf spot, caused by bipolaris sorokiniana, in tall fescue (festuca arundinacea) using the bacterium lysobacter enzymogenes strain c3. resistance elicited by c3 suppressed germination of b. sorokiniana conidia on the phylloplane in addition to reducing the severity of leaf spot. the pathogen-inhibitory effect could be separated from antibiosis by using heat-inactivated cells of c3 that retained no antifungal activi ... | 2003 | 18944093 |
| an antibiotic complex from lysobacter enzymogenes strain c3: antimicrobial activity and role in plant disease control. | lysobacter enzymogenes c3 is a bacterial biological control agent that exhibits antagonism against multiple fungal pathogens. its antifungal activity was attributed in part to lytic enzymes. in this study, a heat-stable antifungal factor (hsaf), an antibiotic complex consisting of dihydromaltophilin and structurally related macrocyclic lactams, was found to be responsible for antagonism by c3 against fungi and oomycetes in culture. hsaf in purified form exhibited inhibitory activity against a wi ... | 2008 | 18944294 |
| lysobacter daecheongensis sp. nov., isolated from sediment of stream near the daechung dam in south korea. | a gram-negative, aerobic, rod shaped, non-spore-forming bacterial strain, designated dae08(t), was isolated from sediment of the stream near daechung dam in south korea, and was characterized in order to determine its taxonomic position, using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain dae08(t) belongs to the family xanthomonadaceae of the gammaproteobacteria, and is related to lysobacter brunescens atcc 29482(t) (97.3%). the phylogenetic distances from ... | 2008 | 18974952 |
| luteimonas aestuarii sp. nov., isolated from tidal flat sediment. | a novel bacterium b9(t) was isolated from tidal flat sediment. its morphology, physiology, biochemical features, and 16s rrna gene sequence were characterized. colonies of this strain are yellow and the cells are gram-negative, rod-shaped, and do not require nacl for growth. the 16s rrna gene sequence similarity indicated that strain b9(t) is associated with the genus lysobacter (< or = 97.2%), xanthomonas (< or = 96.8%), pseudomonas (< or = 96.7%), and luteimonas (< or = 96.0%). however, within ... | 2008 | 18974953 |
| a new type of tripropeptin with anteiso-branched chain fatty acid from lysobacter sp. bmk333-48f3. | branched chain amino acids are often utilized as the precursors of many lipid-containing bacterial secondary metabolites. the effect of isoleucine on the composition of the mixture of cyclic lipopeptide antibiotics, tripropeptins from lysobacter sp. bmk333-48f3 was evaluated. as expected, a novel tripropeptin analog with an anteiso-branched fatty acid was produced. the new compound, tppaic shows potent antibacterial activity against gram-positive bacteria including mrsa and vre. on the other han ... | 2008 | 19160527 |
| novel marine carbazole-degrading bacteria. | eleven carbazole (car)-degrading bacterial strains were isolated from seawater collected off the coast of japan using two different media. seven isolates were shown to be most closely related to the genera erythrobacter, hyphomonas, sphingosinicella, caulobacter, and lysobacter. meanwhile, strains oc3, oc6s, oc9, and oc11s showed low similarity to known bacteria, the closest relative being kordiimonas gwangyangensis gw14-5 (90% similarity). southern hybridization analysis revealed that only five ... | 2009 | 19187209 |
| bacterial diversity associated with ornithogenic soil of the ross sea region, antarctica. | in the ross sea region of antarctica, ornithogenic soils form on land under adélie penguin rookeries. compared with mineral soils of the ross sea region, ornithogenic soils are generally high in microbial biomass, organic carbon, and total nitrogen and phosphorus, with high electrical conductivity and large variations in ph. the objective of this study was to assess the bacterial composition of ornithogenic soils from cape hallett and cape bird in the ross sea region using culture-independent me ... | 2009 | 19190698 |
| influence of lysobacter enzymogenes strain c3 on nematodes. | chitinolytic microflora may contribute to biological control of plant-parasitic nematodes by causing decreased egg viability through degradation of egg shells. here, the influence of lysobacter enzymogenes strain c3 on caenorhabditis elegans, heterodera schachtii, meloidogyne javanica, pratylenchus penetrans, and aphelenchoides fragariae is described. exposure of c. elegans to l. enzymogenes strain c3 on agar resulted in almost complete elimination of egg production and death of 94% of hatched j ... | 2006 | 19259452 |
| proliferation of antibiotic-producing bacteria and concomitant antibiotic production as the basis for the antibiotic activity of jordan's red soils. | anecdotes, both historical and recent, recount the curing of skin infections, including diaper rash, by using red soils from the hashemite kingdom of jordan. following inoculation of red soils isolated from geographically separate areas of jordan, micrococcus luteus and staphylococcus aureus were rapidly killed. over the 3-week incubation period, the number of specific types of antibiotic-producing bacteria increased, and high antimicrobial activity (mic, approximately 10 microg/ml) was observed ... | 2009 | 19286796 |
| lysobacter oryzae sp. nov., isolated from the rhizosphere of rice (oryza sativa l.). | the taxonomic position of a novel bacterial strain, yc6269(t), isolated from the rhizosphere of rice (oryza sativa l.) managed under no-tillage practice in jinju, south korea, was studied using polyphasic approach. cells of the strain were gram-negative, rod-shaped and facultatively anaerobic. the novel strain grew at a temperature of 15-42 degrees c (optimum at 28 degrees c). growth of the strain occurred between ph 5.5 and 11.0, with an optimum at ph 7.0-8.0. the g+c content of the total dna w ... | 2009 | 19329586 |
| lysobacter ximonensis sp. nov., isolated from soil. | a bacterial isolate, strain xm415(t), obtained from soil of tibet in china was characterized using a polyphasic taxonomic approach. strain xm415(t) was aerobic, gram-negative, gliding, rod-shaped, oxidase-negative and catalase-positive. strain xm415(t) showed the highest 16s rrna gene sequence similarity with lysobacter niastensis gh41-7(t) (96.0 %). ubiquinone q-8 and branched fatty acids, such as iso-c(16 : 0) (24.0 %), iso-c(15 : 0) (22.6 %), iso-c(17 : 1)omega9c (6.7 %), iso-c(14 : 0) (6.1 % ... | 2009 | 19329607 |
| effect of salt concentration in anammox treatment using non woven biomass carrier. | effect of high salt concentration on the anammox treatment was investigated to establish an acclimation strategy under high salt concentration conditions. an anammox fixed-bed reactor with non-woven biomass carrier was used and the salt concentration was gradually increased from 2.5 g l(-1) to 33 g l(-1). the anammox reactor demonstrated stable nitrogen removal rate (nrr) of 1.7 kg-n m(-3) d(-1) for 65 days under a salt concentration of 30 g l(-1). however, the nrr sharply declined at a salt con ... | 2009 | 19393551 |
| lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. | a gram-negative, aerobic, rod-shaped, non-spore-forming bacterial strain, designated gsoil 068(t), was isolated from soil of a ginseng field in pocheon province (south korea), and was characterized to determine its taxonomic position by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain gsoil 068(t) belonged to the family xanthomonadaceae, class gammaproteobacteria, and was related most closely to lysobacter brunescens atcc 29482(t) and lysobacter gummosu ... | 2009 | 19406775 |
| isolation and characterization of a car gene cluster from the naphthalene, phenanthrene, and carbazole-degrading marine isolate lysobacter sp. strain oc7. | the novel carbazole (car)-degrading bacterium lysobacter sp. strain oc7 has been isolated from seawater and can also utilize naphthalene and phenanthrene as its sole carbon and energy source. the car-degradative gene cluster was isolated and encoded five complete open reading frames (orfs) and two truncated orfs. among them, four orfs showed 40-50% similarity with previously reported car-degradative genes. ferredoxin (carac) and ferredoxin reductase (carad) genes, which are necessary for the car ... | 2009 | 19452212 |
| arenimonas oryziterrae sp. nov., isolated from a field of rice (oryza sativa l.) managed under a no-tillage regime, and reclassification of aspromonas composti as arenimonas composti comb. nov. | the taxonomic position of a novel bacterial strain, yc6267(t) isolated from a field of rice (oryza sativa l.) managed under a no-tillage regime in jinju, korea, was studied using a polyphasic taxonomic approach. cells of the strain were gram-stain-negative, rod-shaped and aerobic. it grew at 15-37 degrees c (optimum at 28 degrees c). growth of the strain occurred between ph 5.0 and 10.0, with an optimum of ph 7.0-8.0. the g+c content of the total dna was 65.8 mol%. the 16s rrna gene sequence of ... | 2009 | 19628604 |
| outer membrane vesicles of lysobacter sp. | | 2009 | 19650306 |
| ecology and characterization of polyhydroxyalkanoate-producing microorganisms on and in plants. | polyhydroxyalkanoates are energy reserve polymers produced by bacteria to survive periods of starvation in natural habitats. little is known about the ecology of polyhydroxyalkanoate-producing bacteria. to analyse the occurrence of this specific group on/in seven different plant species, a combined strategy containing culture-dependent and -independent methods was applied. using microbial fingerprint techniques (single-strand conformation polymorphism analysis with specific primers for phac gene ... | 2009 | 19656194 |
| 'lysobacter enzymogenes ssp. cookii ' christensen 1978 should be recognized as an independent species, lysobacter cookii sp. nov. | 'lysobacter enzymogenes ssp. cookii' was proposed by christensen and cook in 1978; however, this subspecies name has not been cited in the approved lists of bacterial names and therefore the nomenclature has not been validated. in our genetic approach to clarify the relationships of the designated type strain of 'l. enzymogenes ssp. cookii' pagu 1119 (genbank accession number atcc29488) within the genus lysobacter revealed that the strain was closely related to lysobacter capsiciyc5194(t) (99.4% ... | 2009 | 19659731 |
| lysobacter soli sp. nov., isolated from soil of a ginseng field. | strain dcy21(t), a gram-negative, gliding and rod-shaped aerobic bacterium was isolated from soil of a ginseng field in the republic of korea and characterized using a polyphasic approach in order to determine its taxonomic position. comparative 16s rrna gene sequence analysis revealed that strain dcy21(t) clustered with the species of the genus lysobacter. it was closely related to lysobacter gummosus lmg 8763(t) (97.9 %), lysobacter capsici yc5194(t) (97.6 %), lysobacter antibioticus dsm 2044( ... | 2010 | 19684312 |
| stenotrophomonas and lysobacter: ubiquitous plant-associated gamma-proteobacteria of developing significance in applied microbiology. | the exploration of new source materials and the use of alternative isolation and identification methods have led to rapid expansion in the knowledge of diversity; in lysobacter, 11 new species having been described since 2005, and in stenotrophomonas with six new species since 2000. the new species of lysobacter, isolated by dilution and direct plating on standard media, differ in several key phenotypic properties from those obtained by enrichment on complex polysaccharides in the original descr ... | 2010 | 19702860 |
| biocontrol ability of lysobacter antibioticus hs124 against phytophthora blight is mediated by the production of 4-hydroxyphenylacetic acid and several lytic enzymes. | several rhizobacteria play a vital role in plant protection, plant growth promotion and the improvement of soil health. in this study, we have isolated a strain of lysobacter antibioticus hs124 from rhizosphere and demonstrate its antifungal activity against various pathogens including phytophthora capsici, a destructive pathogen of pepper plants. l. antibioticus hs124 produced lytic enzymes such as chitinase, beta-1,3-glucanase, lipase, protease, and an antibiotic compound. this antibiotic comp ... | 2009 | 19727949 |
| specific detection of lysobacter enzymogenes (christensen and cook 1978) strain 3.1t8 with taqman pcr. | to develop a strain-specific taqman pcr method for detecting and quantifying the biocontrol strain lysobacter enzymogenes 3.1t8. | 2010 | 19732213 |
| aspects on evolution of fungal beta-lactam biosynthesis gene clusters and recruitment of trans-acting factors. | penicillins and cephalosporins are beta-lactam antibiotics. the formation of hydrophobic penicillins has been reported in fungi only, notably penicillium chrysogenum and aspergillus (emericella) nidulans, whereas the hydrophilic cephalosporins are produced by both fungi, e.g., acremonium chrysogenum (cephalosporin c), and bacteria. the producing bacteria include gram-negatives and gram-positives, e.g., streptomyces clavuligerus (cephamycin c) and lysobacter lactamgenus (cephabacins), respectivel ... | 2009 | 19863978 |
| molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques. | polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ... | 2010 | 20018377 |
| detection of 2,4,6-trinitrotoluene-utilizing anaerobic bacteria by 15n and 13c incorporation. | 2,4,6-trinitrotoluene ((15)n or (13)c labeled) was added to norfolk harbor sediments to test whether anaerobic bacteria use tnt for growth. stable-isotope probing (sip)-terminal restriction fragment length polymorphism (trflp) detected peaks in the [(15)n]tnt cultures (60, 163, and 168 bp). the 60-bp peak was also present in the [(13)c]tnt cultures and was related to lysobacter taiwanensis. | 2010 | 20081008 |
| lysobacter xinjiangensis sp. nov., a moderately thermotolerant and alkalitolerant bacterium isolated from a gamma-irradiated sand soil sample. | a yellow-pigmented bacterial strain, designated rcml-52(t), was isolated from an abandoned gold mine in the desert in xinjiang, china. strain rcml-52(t) was gram-negative, aerobic and non-motile. phylogenetic analysis based on 16s rrna gene sequences showed that strain rcml-52(t) was affiliated with the genus lysobacter. strain rcml-52(t) exhibited <95.6 % 16s rrna gene sequence similarity to the type strains of all species of the genus lysobacter. the major fatty acids were iso-c(16 : 0) (27.6 ... | 2011 | 20348320 |
| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| genotypic and phenotypic variation among lysobacter capsici strains isolated from rhizoctonia suppressive soils. | four gram-negative bacterial strains, recovered from clay soils cultivated with different crops in the netherland, were subjected to a polyphasic taxonomic study in order to clarify their taxonomic status. comparative analysis of the 16s rrna gene sequences revealed that they belong to the genus lysobacter and to be highly related to the type strains of l. antibioticus dsm 2044(t), l. gummosus dsm 6980(t), and l. capsici dsm 19286(t), displaying 99.1-99.3%, 99.2-99.6% and 99.4-100% sequence simi ... | 2010 | 20399056 |
| lysobacter ruishenii sp. nov., a chlorothalonil-degrading bacterium isolated from a long-term chlorothalonil-contaminated soil. | an aerobic, gram-negative bacterial strain, designated ctn-1(t), capable of degrading chlorothalonil was isolated from a long-term chlorothalonil-contaminated soil in china, and was subjected to a polyphasic taxonomic investigation. strain ctn-1(t) grew at 15-37 °c (optimum 28-30 °c) and at ph 6.0-9.0 (optimum ph 7.0-7.5). the g+c content of the total dna was 67.1 mol%. based on 16s rrna gene sequence analysis, strain ctn-1(t) was related most closely to lysobacter daejeonensis dsm 17634(t) (97. ... | 2011 | 20400661 |
| molecular detection of transcriptionally active bacteria from failed prosthetic hip joints removed during revision arthroplasty. | the purpose of this study was to use microbiological culture and bacterial 16s rrna gene sequencing methods to detect transcriptionally active bacteria present on the surface of failed prosthetic hip joints removed during revision arthroplasty. five failed prosthetic hip joints were sonicated to dislodge adherent bacteria and subjected to microbiological culture. bacterial rna was extracted from each sonicate, cdna prepared by reverse transcription and the 16s rrna gene amplified using universal ... | 2010 | 20449620 |
| novel strains isolated from a coastal aquifer suggest a predatory role for flavobacteria. | three newly isolated strains of flavobacteria from coastal aquifer sediments have been found to be predatory, lysing a range of live and pasteurized microbial prey. the three strains have been classified on the basis of 16s rrna gene phylogeny as belonging to the recently described olleya (strains vcsa23 and vcsm12) and tenacibaculum (strain vcsa14a) genera. two of the closest cultured relatives to the strain vcsa14a, tenacibaculum discolor and tenacibaculum gallaicum, were also found to be bact ... | 2010 | 20500528 |
| lysobacter dokdonensis sp. nov., isolated from soil. | a gram-negative, non-motile, rod-shaped bacterial strain, ds-58(t), was isolated from a soil sample from dokdo, an island of korea, and its taxonomic position was investigated. phylogenetic analysis based on 16s rrna gene sequences showed that strain ds-58(t) fell within the family xanthomonadaceae. the isolate showed 96.9 % 16s rrna gene sequence similarity with its closest phylogenetic neighbour, lysobacter niastensis gh41-7(t), and 93.4-95.7 % 16s rrna gene sequence similarity with other memb ... | 2011 | 20525815 |
| ammonia accumulation in culture broth by the novel nitrogen-fixing bacterium, lysobacter sp. e4. | this is the first report that lysobacter fixes nitrogen under free-living conditions, as shown by its ability to grow on nitrogen-free medium and accumulate relatively high amounts of ammonia in the culture broth. growth of the e4 lysobacter strain, isolated in a screen for nitrogen-fixing and ammonia-producing bacteria, resulted in higher ammonia accumulation (0.53 mm ammonium ion concentration) in media containing glucose rather than other tested carbon sources. the optimum glucose concentrati ... | 2010 | 20573544 |
| a novel laccase with urate oxidation activity from lysobacter sp. t-15. | a unique urate-oxidizing enzyme was identified in a bacterium, strain t-15. based on its phylogenetic, physiological and biochemical properties, strain t-15 was deemed to be a novel species within the genus lysobacter. the enzyme expressed in lysobacter sp. t-15 was composed of 592 amino acids and contained four consensus copper-binding sites, and the recombinant enzyme was, at least in this study, speculated to have three cu ions per subunit. the primary structure of the enzyme was 33% identica ... | 2010 | 20675295 |
| postbiostimulation microbial community structure changes that control the reoxidation of uranium. | this study evaluated the influence of changes in the microbial community structure on reoxidation of reduced uranium during a postbiostimulation period. effluent groundwater from acetate-stimulated sediment flow-through columns was analyzed over 60 days after acetate amendment was discontinued. only a small reoxidation of iron or uranium (17%) occurred in the presence of 1-2 mg l(-1) o(2) influent groundwater for the 2-month period. most uranium reoxidation occurred during the first 2 weeks afte ... | 2010 | 20707815 |
| mapping the eosinophil cationic protein antimicrobial activity by chemical and enzymatic cleavage. | the eosinophil cationic protein (ecp) is a human antimicrobial protein involved in the host immune defense that belongs to the pancreatic rnase a family. ecp displays a wide range of antipathogen activities. the protein is highly cationic and its bactericidal activity is dependant on both cationic and hydrophobic surface exposed residues. previous studies on ecp by site-directed mutagenesis indicated that the rnase activity is not essential for its bactericidal activity. to further understand th ... | 2010 | 20951760 |
| lysobacter korlensis sp. nov. and lysobacter bugurensis sp. nov., isolated from soil in north-west china. | two gram-negative, rod-shaped, gliding and yellow-pigmented bacterial strains, designated zld-17(t) and zld-29(t), were isolated from arid soil samples collected from xinjiang province, north-west china, and subjected to a polyphasic taxonomic study. both novel strains requires 1-2% (w/v) sea salts for optimal growth. phylogenetic analysis based on 16s rrna gene sequences indicated that these two strains belong to the genus lysobacter within the gammaproteobacteria. strain zld-17(t) showed the h ... | 2010 | 20952549 |
| cloning of the chlorothalonil-degrading gene cluster and evidence of its horizontal transfer. | strain ochrobactrum lupine tp-d1 was found to degrade chlorothalonil (tpn) to 4-hydroxy-chlorothalonil (tpn-oh). to clone the related degrading gene, genomic library of tp-d1 was constructed using escherichia coli dh10b and two positive clones 889 and 838 were gained. however, no plasmid was detected in clone 889. and in clone 838, a 3494 bp fragment was cloned which contains a 984 bp hydrolytic dehalogenase (chd) gene and a 1926 bp insertion element is-olup. the insertion element contains a tra ... | 2010 | 21127873 |
| biosynthesis of hsaf, a tetramic acid-containing macrolactam from lysobacter enzymogenes. | hsaf was isolated from lysobacter enzymogenes , a bacterium used in the biological control of fungal diseases of plants. structurally, it is a tetramic acid-containing macrolactam fused to a tricyclic system. hsaf exhibits a novel mode of action by disrupting sphingolipids important to the polarized growth of filamentous fungi. here we describe the hsaf biosynthetic gene cluster, which contains only a single-module polyketide synthase/nonribosomal peptide synthetase (pks/nrps), although the bios ... | 2010 | 21171605 |
| stimulants of toll-like receptors 2 and 4 are elevated in saliva of periodontitis patients compared with healthy subjects. | because the absorption of stimulants of toll-like receptor (tlr)2 and tlr4 from the gastrointestinal tract into the circulation has been proposed to promote the development of atherosclerosis and insulin resistance, we aimed to quantify the abundance of stimulants of tlr2 and tlr4 in human saliva. | 2011 | 21284689 |
| the microbiota of an unpolluted calcareous soil faces up chlorophenols: evidences of resistant strains with potential for bioremediation. | to highlight the effects of a variety of chlorophenols (cp) in relation to the response of an indigenous bacterial community, an agricultural mediterranean calcareous soil has been studied in microcosms incubated under controlled laboratory conditions. soil samples were artificially polluted with 2-monochlorophenol (mcp), 2,4,6-trichlorophenol (tcp) and pentachlorophenol (pcp), at concentrations ranging from 0.1 up to 5000 mg kg(-1). both activity and composition of the microbial community were ... | 2011 | 21295817 |
| a new anti-mrsa antibiotic complex, wap-8294a ii. structure characterization of minor components by esi lcms and ms/ms. | the anti-mrsa antibiotic, wap-8294a, was isolated from the fermentation broth of lysobacter sp. the major component, wap-8294a2, is composed of 1 mol of gly, l-leu, l-glu, d-asn, d-trp, d-threo-ß-hydroxyasparagine, n-me-d-phe and n-me-l-val, and 2?mol of l-ser, d-orn and d-3-hydroxy-7-me-octanoic acid. the structure of the wap-8294a2 was mainly determined as a cyclic depsipeptide by 2d nmr experiments. however, it was difficult to use the nmr experiment to determine the minor components, a1, a4 ... | 2011 | 21326252 |
| enzymes hydrolyzing structural components and ferrous ion cause rusty-root symptom on ginseng (panax ginseng). | microbial induction of rusty-root was proved in this study. the enzymes hydrolyzing plant structural materials, including pectinase, pectolyase, ligninase, and cellulase, caused the rusty-root in ginseng. pectinase and pectolyase produced the highest rusty-color formation. ferrous ion (fe+++) caused the synergistic effect on rusty-root formation in ginseng when it was used with pectinase. the effect of ferric ion (fe++) on rusty-root formation was slow, compared with fe+++, probably due to gradu ... | 2011 | 21364303 |
| root treatment with rhizobacteria antagonistic to phytophthora blight affects anthracnose occurrence, ripening, and yield of pepper fruit in the plastic house and field. | we previously selected rhizobacterial strains ccr04, ccr80, gse09, ise13, and ise14, which were antagonistic to phytophthora blight of pepper. in this study, we investigated the effects of root treatment of rhizobacteria on anthracnose occurrence, ripening, and yield of pepper fruit in the plastic house and field in 2008 and 2009. we also examined the effects of volatiles produced by the strains on fruit ripening and on mycelial growth and spore development of colletotrichum acutatum and phytoph ... | 2011 | 21405997 |
| bacterial communities associated with chenopodium album and stellaria media seeds from arable soils. | the bacterial community compositions in chenopodium album and stellaria media seeds recovered from soil (soil weed seedbank), from bulk soil, and from seeds harvested from plants grown in the same soils were compared. it was hypothesized that bacterial communities in soil weed seedbanks are distinct from the ones present in bulk soils. for that purpose, bacterial polymerase chain reaction denaturing gradient gel electrophoresis (pcr-dgge) fingerprints, made from dna extracts of different soils a ... | 2011 | 21424277 |
| indigenous populations of three closely related lysobacter spp. in agricultural soils using real-time pcr. | previous research had shown that three closely related species of lysobacter, i.e., lysobacter antibioticus, lysobacter capsici, and lysobacter gummosus, were present in different rhizoctonia-suppressive soils. however, the population dynamics of these three lysobacter spp. in different habitats remains unknown. therefore, a specific primer-probe combination was designed for the combined quantification of these three lysobacter spp. using taqman. strains of the three target species were efficien ... | 2011 | 21448673 |
| induction of cell wall thickening by the antifungal compound dihydromaltophilin disrupts fungal growth and is mediated by sphingolipid biosynthesis. | dihydromaltophilin (heat-stable antifungal factor [hsaf]) is an antifungal metabolite produced in lysobacter enzymogenes biocontrol strain c3. this compound induces cell wall thickening in aspergillus nidulans. here we show that the cell wall thickening is a general response to hsaf in diverse fungal species. in the a. nidulans model, the thickened cell wall negatively affects hyphal growth. growth of hsaf-pre-treated hyphae failed to resume at hyphal tips with thick cell wall and the actin cabl ... | 2009 | 21462551 |
| diversity of culturable chitinolytic bacteria from rhizospheres of agronomic plants in japan. | a total of 100 isolates of chitinolytic bacteria were obtained from the rhizospheres of various agronomic plants, and the 16s rrna gene sequences of these isolates were determined. phylogenetic analyses revealed that 81 isolates belonged to the classes betaproteobacteria (39 isolates) and gammaproteobacteria (42 isolates). of the remaining 19 isolates, 16 belonged to the phylum firmicutes. clustering analysis identified 6 and 3 operational taxonomic units (otus) in gammaproteobacteria and betapr ... | 2011 | 21487197 |
| identification and characterization of the lysobactin biosynthetic gene cluster reveals mechanistic insights into an unusual termination module architecture. | lysobactin (katanosin b) is a macrocyclic depsipeptide, displaying high antibacterial activity against human pathogens. in this work, we have identified and characterized the entire biosynthetic gene cluster responsible for lysobactin assembly. sequential analysis of the lysobacter sp. atcc 53042 genome revealed the lysobactin gene cluster to encode two multimodular nonribosomal peptide synthetases. as the number of modules found within the synthetases lyba and lybb directly correlates with the ... | 2011 | 21609846 |
| tripropeptin c blocks the lipid cycle of cell wall biosynthesis by complex formation with undecaprenyl pyrophosphate. | tripropeptin c (tppc) is a naturally occurring cyclic lipodepsipeptide antibiotic produced by lysobacter sp. tppc exhibits potent antibacterial activity against methicillin-resistant staphylococcus aureus (mrsa), vancomycin-resistant enterococci (vre) and penicillin-resistant streptococcus pneumoniae. this antibiotic also inhibits the incorporation of n-acetylglucosamine into the peptidoglycan of s. aureus at a 50% inhibitory concentration (ic(50)) of 0.7 μm, which is proportional to its mic (0. ... | 2011 | 21628543 |
| separation of fluorescence-labelled terminal restriction fragment dna on a two-dimensional gel (t-rfs-2d) - an efficient approach for microbial consortium characterization. | fingerprinting techniques provide access to understanding the ecology of uncultured microbial consortia. however, the application of current techniques such as terminal restriction fragment length polymorphism (t-rflp) and denaturing gradient gel electrophoresis (dgge) has been hindered due to their limitations in characterizing complex microbial communities. this is due to that different populations possibly share the same terminal restriction fragments (t-rfs) and dna fragments may co-migrate ... | 2011 | 21824243 |
| Identification and characterization of the anti-methicillin-resistant Staphylococcus aureus WAP-8294A2 biosynthetic gene cluster from Lysobacter enzymogenes OH11. | Lysobactor enzymogenes strain OH11 is an emerging biological control agent of fungal and bacterial diseases. We recently completed its genome sequence and found it contains a large number of gene clusters putatively responsible for the biosynthesis of nonribosomal peptides and polyketides, including the previously identified antifungal dihydromaltophilin (HSAF). One of the gene clusters contains two huge open reading frames, together encoding 12 modules of nonribosomal peptide synthetases (NRPS) ... | 2011 | 21930890 |
| lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. | a gram-negative, aerobic, motile, rod-shaped, arsenite [as(iii)]-resistant bacterium, designated strain zs79(t), was isolated from subsurface soil of an iron mine, in china. phylogenetic analyses based on 16s rrna gene sequences revealed that strain zs79(t) was closely clustered with five lysobacter species, with 96.9, 96.1, 96.0, 95.8 and 95.3% sequence similarities to l. concretionis ko07(t), l. daejeonensis gh1-9(t), l. defluvii immib apb-9(t), l. spongiicola kmm 329(t) and l. ruishenii ctn-1 ... | 2011 | 21890727 |
| unusual activities of the thioesterase domain for the biosynthesis of the polycyclic tetramate macrolactam hsaf in lysobacter enzymogenes c3. | hsaf is an antifungal natural product with a new mode of action. a rare bacterial iterative pks-nrps assembles the hsaf skeleton. the biochemical characterization of the nrps revealed that the thioesterase (te) domain possesses the activities of both a protease and a peptide ligase. active site mutagenesis, circular dichroism spectra and homology modeling of the te structure suggested that the te may possess uncommon features that may lead to the unusual activities. the iterative pks-nrps is fou ... | 2011 | 22182183 |
| performance and bacterial compositions of aged refuse reactors treating mature landfill leachate. | aged landfill leachates become more refractory over time and difficulty to treat. recently, aged refuse bioreactors show great promise in treating leachates. in this study, aged refuse bioreactors were constructed to simulate landfill leachate degradation process. the characteristics of leachate were: codcr, ∼2200 mg/l; bod5, ∼280 mg/l; total nitrogen, ∼2030 mg/l; and ammonia, ∼1900 mg/l. results showed that bioreactor could remove leachate pollutants effectively at hydraulic loading of 20 l/m3 ... | 2012 | 22023964 |
| microbial diversity inside pumpkins: microhabitat-specific communities display a high antagonistic potential against phytopathogens. | recent and substantial yield losses of styrian oil pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) are primarily caused by the ascomycetous fungus didymella bryoniae but bacterial pathogens are frequently involved as well. the diversity of endophytic microbial communities from seeds (spermosphere), roots (endorhiza), flowers (anthosphere), and fruits (carposphere) of three different pumpkin cultivars was studied to develop a biocontrol strategy. a multiphasic approach combining molec ... | 2011 | 21947430 |
| monoclonal antibodies against the propeptide of endopeptidase alpb of lysobacter sp. xl1 for studying alpb-protein interactions in bacterial cells. | | 2012 | 22218960 |