| cell surface hydrophobicity, adherence to hela cell cultures and haemagglutination pattern of pyelonephritogenic escherichia coli strains. | cell surface hydrophobicity, haemagglutination pattern and adherence to hela cells were examined in 230 strains of escherichia coli collected from women (n = 61 strains) and children (n = 65 strains) with non-obstructive acute pyelonephritis and in 104 faecal control strains of e. coli from healthy adults (n = 71 strains) and children (n = 33 strains). pyelonephritogenic e. coli strains showed a significantly increased incidence of hydrophobic properties (90%) and mannose resistant haemagglutina ... | 1990 | 2209732 |
| construction and properties of active chimeric enzymes between human aldolases a and b. analysis of molecular regions which determine isozyme-specific functions. | to study the structure/function relationships of human aldolase isozymes, particularly isozyme-specific functions, we constructed escherichia coli expression plasmids for six ba chimeric enzymes (ba34, ba108, ba137, ba212, ba306, and ba306*), each composed of the n-terminal side of isozyme b and the c-terminal side of isozyme a, and one bab chimeric enzyme which contains a fragment of isozyme a (residues 213-306) inserted in between the n-terminal and the c-terminal fragments of isozyme b. they ... | 1990 | 2211642 |
| endothelial and leukocyte forms of il-8. conversion by thrombin and interactions with neutrophils. | we have recently shown that endothelial cell-derived il-8 inhibits neutrophil adhesion to il1-beta-activated human umbilical vein endothelial cell monolayers. il-8 secreted by t lymphocytes or monocytes has been characterized as a promoter of neutrophil degranulation and chemotaxis. the il-8 isolated from each of these cell types is a mixture of two il-8 polypeptides, one consisting of 72 amino acids (herein called [ser-il-8]72) and the other 77 amino acids (an n-terminal extended form herein ca ... | 1990 | 2212672 |
| purification and characterization of recombinant rev protein of human immunodeficiency virus type 1. | recombinant rev protein of human immunodeficiency virus type 1 has been expressed in escherichia coli and purified by ion-exchange and gel-filtration chromatography. specific binding of the purified protein to the rev-responsive element of the viral rna is demonstrated. physical characterization of the purified protein by circular dichroism and intrinsic fluorescence spectroscopy indicate that the protein preparation is suitable for structural analysis. circular dichroism measurements show that ... | 1990 | 2217189 |
| expression of active human hypoxanthine-guanine phosphoribosyltransferase in escherichia coli and characterisation of the recombinant enzyme. | a plasmid, prg1, has been constructed by incorporating the coding sequence of human hypoxanthine-guanine phosphoribosyltransferase (hprt) into the expression vector pt7-7. expression of human hprt has been achieved in hprt- escherichia coli cells transformed with prg1 and pgp1-2, as shown by: (1) exclusive labelling with [35s]methionine of a polypeptide with the same mobility as purified human hprt on sds-page; and (2) measurement of hprt activity after cell lysis. although the majority of the r ... | 1990 | 2223882 |
| response of tumors to thermodynamic stimulation of the immune system. | cytokines such as interferon, interleukin and tumor necrosis factor are natural body defense proteins which have been used individually in recent years to produce a few complete responses of some tumors in a few patients but their overall effect has been limited. the hypotheses is that a biologic stimulus such as endotoxin will stimulate the immune system in a more natural way and hence will be more likely to have an effect especially in the presence of a naturally produced fever than treatment ... | 1990 | 2226999 |
| factors and markers of virulence in escherichia coli from human septicemia. | one hundred escherichia coli isolates from human septicemia were characterized with respect to o serogroups 1, 2, 4, 6, 7, 8, 15, 18, 75 and 78, alpha-hemolysin, carboxylesterase b typing, cytotoxic necrotizing factor, f165 and cs31a fimbrial antigens, aerobactin production, colicins, and antibiotic sensitivity. a factorial analysis of correspondence and chi 2 tests indicated that most of e. coli isolates belonging to the studied o serogroups were positive for the virulence factors or markers al ... | 1990 | 2227363 |
| binding of sodium dodecyl sulphate to an integral membrane protein and to a water-soluble enzyme. determination by molecular-sieve chromatography with flow scintillation detection. | we have determined the binding of sodium dodecyl sulphate (sds) to the human red cell glucose transporter (polypeptide, mr 54,117) and to a water-soluble enzyme, n-5'-phosphoribosylanthranilate isomerase-indole-3-glycerol-phosphate synthase (prai-igps) from escherichia coli (mr 49,484). [35s]sds was equilibrated with each protein on molecular-sieve chromatography at a series of sds concentrations. the binding ratios of sds to protein were determined by flow scintillation detection and automated ... | 1990 | 2229232 |
| fucosylated oligosaccharides of human milk protect suckling mice from heat-stabile enterotoxin of escherichia coli. | human milk protects suckling mice from the diarrheagenic effects of heat-stabile enterotoxin of escherichia coli (st). to identify the human milk fraction responsible for this protection, pooled skimmed, deproteinated milk was passed through charcoal, whereupon lactose was separated from the oligosaccharides. the oligosaccharides contained st-protective activity; the lactose did not. the neutral, but not the acidic, fraction exhibited protective activity against st (22% vs. 57% mortality, respec ... | 1990 | 2230234 |
| prooxidant activity of transferrin and lactoferrin. | acceleration of the autoxidation of fe2+ by apotransferrin or apolactoferrin at acid ph is indicated by the disappearance of fe2+, the uptake of oxygen, and the binding of iron to transferrin or lactoferrin. the product(s) formed oxidize iodide to an iodinating species and are bactericidal to escherichia coli. toxicity to e. coli by feso4 (10(-5) m) and human apotransferrin (100 micrograms/ml) or human apolactoferrin (25 micrograms/ml) was optimal at acid ph (4.5-5.0) and with logarithmic phase ... | 1990 | 2230644 |
| embryonic neural cell adhesion molecule in cerebrospinal fluid of younger children: age-dependent decrease during the first year. | poly-alpha-2,8-n-acetylneuraminic acid (poly-alpha-2,8-neuac) is developmentally expressed in neural tissue of higher animals, where it is covalently attached to the neural cell adhesion molecule (ncam), a large integral membrane glycoprotein mediating cell-cell adhesion during neuronal development. ncam exists in several molecular forms, of which only embryonic ncam carries lengthy chains (n greater than 5) of poly-alpha-2,8-neuac. chemically identical poly-alpha-2,8-neuac of bacterial origin i ... | 1990 | 2230809 |
| photoaffinity labeling of human c-myc protein with deoxythymidine triphosphate. | the recombinant human c-myc protein expressed in escherichia coli can be efficiently labeled by ultraviolet-mediated cross-linking to dttp and to a lesser extent to other nucleoside diphosphates and triphosphates, but not to nucleoside monophosphates. specificity of nucleoside phosphate binding is suggested by (a) concentration-dependent competition by some nucleoside phosphates but not by others and (b) by the observation that the denatured myc protein does not bind the nucleotides. competition ... | 1990 | 2232707 |
| expression of human 21-hydroxylase (p450c21) in bacterial and mammalian cells: a system to characterize normal and mutant enzymes. | cytochrome p450c21 (steroid 21-hydroxylase) is a key enzyme in the synthesis of cortisol, whose deficiency is the cause of a common genetic disease, congenital adrenal hyperplasia. we have expressed p450c21 (steroid 21-hydroxylase) in e. coli and mammalian cells. in e. coli, p450c21 cdna was cloned into a t7 expression vector to produce a large amount of p450c21 fusion protein, which enabled antiserum production. in mammalian cells, a plasmid containing full-length p450c21 cdna (phc21) was const ... | 1990 | 2233746 |
| synthesis of a gene for human serum albumin and its expression in saccharomyces cerevisiae. | a 1761 base pairs long artificial gene coding for human serum albumin (hsa) has been prepared by a newly developed synthetic approach, resulting in the largest synthetic gene so far described. oligonucleotides corresponding to only one strand of the hsa gene were prepared by chemical synthesis, while the complementary strand was obtained by a combination of enzymatic and cloning steps. 24 synthetic, 69-85 nucleotides long oligonucleotides covering the major part of the hsa gene (41-1761 nucleoti ... | 1990 | 2235491 |
| emphysematous gastritis: case report and review. | emphysematous gastritis is a condition involving gastric wall inflammation, radiologic or intraoperative evidence of intramural gas, and systemic toxicity. a recent case of emphysematous gastritis in a 57-year-old diabetic man is reported, and 27 cases published since 1889 are reviewed. predisposing factors include ingestion of corrosive substances (37%) and alcohol abuse (22%). diagnosis of emphysematous gastritis is based on the clinical presentation of an acute abdomen with systemic toxicity ... | 1990 | 2237128 |
| human immunodeficiency viral protease is catalytically active as a fusion protein: characterization of the fusion and native enzymes produced in escherichia coli. | processing of the gag and pol gene precursor proteins of retroviruses is essential for the production of mature infectious virions. the processing is directed by a viral protease that itself is part of these precursors and is presumed to cleave itself autocatalytically. to facilitate study of this process, the protease was produced as a fusion protein in escherichia coli. in this construct, the 10,793-da protease was preceeded by two copies of a modified igg binding domain derived from protein a ... | 1990 | 2241167 |
| in vitro cytotoxic effect of alpha-hemolytic escherichia coli on human blood granulocytes. inhibition by alpha-hemolysin antibody. | the influence of alpha-hemolysin antibody on the in vitro cytotoxic effect of alpha-hemolytic escherichia coli bacteria and culture filtrates was investigated. damage to human blood granulocytes was quantified by measuring the release of chromium 51 from labelled cells in the presence of whole or fractionated plasma containing alpha-hemolysin antibody. anti-alpha-hemolysin activity was found exclusively in the igg fraction of plasma. human plasma contained "natural" alpha-hemolysin antibody to v ... | 1990 | 2248684 |
| extended phage-typing scheme for escherichia coli o157:h7. | in canada, the number of human isolates of verotoxigenic (vt + ve) escherichia coli o157:h7 from diarrhoeal cases and haemolytic uraemic syndrome and haemorrhagic colitis has increased from 25 in 1982 to 2384 in 1989. a total of 3273 vt + ve e. coli o157:h7 strains (3255 strains isolated in canada and 18 isolates from other countries) were phage typed. the phage typing scheme has been extended from 14 to 62 phage types. of these, five types occurred exclusively in other countries (type 47 in jap ... | 1990 | 2249715 |
| interactions of escherichia coli and proteus mirabilis with mouse mononuclear phagocytes. | five strains of enterobacteria (three of escherichia coli and two of proteus mirabilis) were studied to assess and compare their phagocytic uptake and intracellular killing by mouse macrophages. each strain was injected intraperitoneally into separate groups of mice and peritoneal exudate cells were harvested after 3 min for phagocytosis to occur in vivo. acridine orange staining showed that there were approximately 10-fold fewer intracellular p. mirabilis than e. coli cells. the average numbers ... | 1990 | 2250285 |
| field trial of oral cholera vaccines in bangladesh: evaluation of anti-bacterial and anti-toxic breast-milk immunity in response to ingestion of the vaccines. | in a field trial conducted in bangladesh, ingestion of either b subunit-killed whole cell (bs-wc) or killed whole cell (wc) oral cholera vaccines by mothers was associated with a 47% reduction of the risk of cholera in their non-vaccinated children aged under 36 months. because vaccine-induced breast-milk immunity seemed a possible explanation for these findings, we evaluated anti-lipopolysaccharide (lps) and anti-cholera toxin (ct) iga antibody responses in breast milk collected during the tria ... | 1990 | 2251873 |
| paf-acether synthesis by helicobacter pylori. | clinical studies suggest that helicobacter pylori may play a role in the pathogenesis of gastroduodenal ulcers in man but direct evidence of mucosal injury by this microorganism is still lacking. paf-acether (paf) causes a number of disorders including ischaemic bowel necrosis and gastroduodenal ulceration. since paf is produced by escherichia coli, we investigated whether it could be synthesised by h pylori. five h pylori isolates were collected from antral biopsy specimens from patients with g ... | 1990 | 2253906 |
| portal venous gas in a patient with diverticulitis. | gas in the portal vein is a rare finding associated with a grave prognosis. we present a case of portal venous gas in a 34-year-old man with an abscess due to perforated-sigmoid diverticulitis and escherichia coli sepsis. successfully treated with sigmoid resection and antibiotic therapy. | 1990 | 2253932 |
| stimulation of granulopoiesis in patients with malignancy by recombinant human granulocyte-macrophage colony-stimulating factor: assessment of two routes of administration. | we administered escherichia coli-derived recombinant human granulocyte-macrophage colony-stimulating factor to 61 patients with malignancy, 36 of whom had normal peripheral blood counts and 25 of whom had peripheral cytopenia due to underlying bone marrow disease, to compare the efficacy of two different routes of administration to stimulate the in vivo granulopoiesis: i.e., continuous i.v. infusion and s.c. injection. three well-tolerated dose levels were investigated. application of granulocyt ... | 1990 | 2254759 |
| an investigation of immunological subclass function in burned adults. | the serum concentrations of the immunoglobulin subclasses and their functional activity against pneumococcal polysaccharide (pps) and e. coli antigens have been studied for 15 days in 12 adult burned patients, of whom six were randomly allocated to receive biosynthetic human growth hormone (somatropin) and six to form a control group. the concentrations of the major subclasses, igg1 and igg2, fell below their normal ranges as a result of injury by burning but had recovered significantly by the e ... | 1990 | 2257072 |
| production of cytotoxic necrotizing factor, verocytotoxin and haemolysin by pyelonephritogenic escherichia coli. | two hundred and thirty-two strains of escherichia coli isolated from children with non-obstructive acute pyelonephritis (n = 65), women with non-obstructive acute pyelonephritis (n = 63) and the faecal flora of healthy children (n = 33) and adults (n = 71) were examined for cytotoxic necrotizing factor production, haemolysin synthesis, verocytotoxin production and expression of mannose-resistant haemaglutination of human erythrocytes. forty-eight per cent of the pyelonephritogenic escherichia co ... | 1990 | 2261921 |
| stability and activity of human immunodeficiency virus protease: comparison of the natural dimer with a homologous, single-chain tethered dimer. | a single-chain tethered dimer of human immunodeficiency virus protease (hiv-pr) was produced by expression of a synthetic gene in escherichia coli. the tethered dimer, which consists of two 99-amino acid hiv-pr subunits linked together by a pentapeptide, was isolated from inclusion bodies and refolded as an active protease with enzymatic properties very similar to those of the natural dimer at ph 5.5. in addition to demonstrating that the tethered dimer is active, we have shown that the tethered ... | 1990 | 2263618 |
| antibodies to hiv-1 nef(p27): prevalence, significance, and relationship to seroconversion. | a sensitive and specific enzyme-linked immunoassay for antibodies to the human immunodeficiency virus type 1 (hiv-1) nef gene product, p27, has been developed using recombinant escherichia coli-derived protein from the lav-1-bru sequence. of 92 hiv-1 infected hemophiliacs, 72 (78%) produced anti-nef antibodies in this assay; the early appearance of anti-nef prior to full seroconversion was a rare event in this population, occurring in only one subject (approximately 1%). anti-nef antibodies were ... | 1990 | 2265027 |
| spermidine biosynthesis in saccharomyces cerevisiae. biosynthesis and processing of a proenzyme form of s-adenosylmethionine decarboxylase. | we have cloned and sequenced the saccharomyces cerevisiae gene for s-adenosylmethionine decarboxylase. this enzyme contains covalently bound pyruvate which is essential for enzymatic activity. we have shown that this enzyme is synthesized as a mr 46,000 proenzyme which is then cleaved post-translationally to form two polypeptide chains: a beta subunit (mr 10,000) from the amino-terminal portion and an alpha subunit (mr 36,000) from the carboxyl-terminal portion. the protein was overexpressed in ... | 1990 | 2266128 |
| expression of human beta-myosin heavy chain fragments in escherichia coli; localization of actin interfaces on cardiac myosin. | a cdna clone coding for an internal fragment of slow-cardiac beta-myosin heavy chain was isolated from a lambda gt10 human skeletal muscle library. six overlapping cdna subclones, which span myosin heavy chain subregions and presumably interact with actin, were derived from this clone, fused to a beta-galactosidase vector and expressed in escherichia coli. three of the subclones were obtained by pcr (polymerase chain reaction) which enables gene or cdna fragments to be amplified independently of ... | 1990 | 2266165 |
| a candida albicans homolog of a human cyclophilin gene encodes a peptidyl-prolyl cis-trans isomerase. | a candida albicans cdna and its genomic counterpart were isolated from lambda phage libraries using a human t-cell cyclophilin (cyp) cdna as a hybridization probe. the clones contain a 486-bp open reading frame predicting a 162-amino acid, approx. 18 kda protein which is similar in size to, and which shares 68 and 81% homology with, human t-cell cyp and cytosolic saccharomyces cerevisiae cyp, respectively. northern blots show the presence of a single mrna species of about 800 bp. however, genomi ... | 1990 | 2269432 |
| association of virulence markers with animal pathogenicity of escherichia coli in different models. | employing chicken and several strains of mice, different routes (intraperitoneal, subcutaneous) of infections and isogenic pairs of strains, association of virulence markers with animal pathogenicity was studied in escherichia coli. mouse virulence of avian strains was less significant than the lethality for chicks of human strains. ld50 in various animals did not differ significantly. strains with antigen k1 were more virulent for mice than their k1- derivatives. loss of haemolysin (hly), manno ... | 1990 | 2270740 |
| comparison of chemical reactivity, cytotoxicity, interstrand cross-linking and dna sequence specificity of bis(platinum) complexes containing monodentate or bidentate coordination spheres with their monomeric analogues. | the properties of a new bis(platinum) complex containing two monodentate coordination spheres, [(trans-ptcl(nh3)2)2h2n(ch2)4nh2]cl2 (1,1/t,t), are reported. comparison is made with respect to chemical reactivity, in vitro biological activity in murine and tumor cells, dna conformational changes, cross-linking efficiency, and sequence specificity between this complex and the previously reported complex containing two bidentate platinum atoms, [(pt(mal)(nh3))2h2n(ch2)4nh2] (2,2/c,c), as well as wi ... | 1990 | 2271599 |
| novel endotoxin adsorbing materials, polymyxin-sepharose and polyporous polyethylene membrane for removal of endotoxin from dialysis systems. | in order to remove contaminated endotoxin from dialysis systems, we prepared and investigated two novel endotoxin adsorbing materials, polymyxin-sepharose (pxseph) and polyporous polyethylene hollow fiber membrane (ehf). pxseph was prepared by covalently immobilizing polymyxin b on sepharose 4b beads by cnbr coupling method. it adsorbed various endotoxins with a high affinity constant and could effectively remove endotoxin from aqueous solution and human plasma. ehf also removed endotoxins from ... | 1990 | 2285811 |
| ampicillin-sulbactam therapy for multiple pyogenic hepatic abscesses. | a patient with multiple, pyogenic hepatic abscesses is described, and the pathophysiology, etiologies, clinical and laboratory manifestations, and management of the disease are reviewed. a 55-year-old man with a history of ethanol abuse and pancreatitis developed fever, chills, general malaise, and right upper quadrant abdominal pain two weeks before hospitalization. baseline laboratory and hematology results included serum albumin concentration, 3.2 g/dl; serum alkaline phosphatase concentratio ... | 1990 | 2292177 |
| thrombopoietic activity of human interleukin-6. | thrombopoietin (tpo), a regulatory factor in platelet production, was purified from the conditioned medium of tnk-01 cells cultured in the presence of human interleukin-1. the n-terminal sequence of purified tpo was determined to be vppgedskdvaaphrqplt, identical to that of the n-terminal region of human interleukin-6 (il-6). two forms of tpo with molecular masses of 24 and 27 kda were identified as il-6 by western analysis using an anti-il-6 antibody. commercial recombinant human il-6 produced ... | 1990 | 2298297 |
| human complex ii (succinate-ubiquinone oxidoreductase): cdna cloning of iron sulfur (ip) subunit of liver mitochondria. | complex ii (succinate-ubiquinone oxidoreductase) is an important enzyme complex of both the tricarboxylic acid cycle and of the aerobic respiratory chains of mitochondria in eukaryotic cell and prokaryotic organisms. in this study, the amino acid sequence of iron sulfur-subunit in human liver mitochondria was deduced from cdna which was isolated by immunoscreening a human liver lambda gtll cdna library. an isolated clone contains an open reading frame of 786 nucleotides and encodes a mature prot ... | 1990 | 2302193 |
| stable transfection of the human parasite leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression. | to delineate segments of the genome of the human protozoan parasite leishmania major necessary for replication and expression, we developed a vector (pr-neo) which can be reproducibly introduced into l. major. this dna was derived from a 30-kilobase extrachromosomal amplified dna bearing the dihydrofolate reductase-thymidylate synthase gene, with the coding region for neomycin phosphotransferase substituted for that of dihydrofolate reductase-thymidylate synthase and a bacterial origin of replic ... | 1990 | 2304458 |
| glucose phosphorylation in tumor cells. cloning, sequencing, and overexpression in active form of a full-length cdna encoding a mitochondrial bindable form of hexokinase. | in rapidly growing tumor cells exhibiting high glucose catabolic rates, the enzyme hexokinase is markedly elevated and bound in large amounts (50-80% of the total cell activity) to the outer mitochondrial membrane (arora, k.k., and pedersen, p.l. (1988) j. biol. chem. 263, 17422-17428; parry, d.m., and pedersen, p.l. (1983) j. biol. chem. 258, 10904-10912). in extending these studies, we have isolated a cdna clone of hexokinase from a lambda gt11 library of the highly glycolytic, c37 mouse hepat ... | 1990 | 2318862 |
| the gene sequence and some properties of protein h. a novel igg-binding protein. | the gene for protein h, a novel bacterial cell wall protein with specific affinity for human igg fc, was cloned from a group a streptococcus and expressed in escherichia coli. recombinant e. coli cells produced two forms of a human igg fc-binding protein, one with an apparent mr of 42 kda in a periplasmic fraction and the other with an apparent mr of 45 kda in a mixed fraction of cytoplasms and membranes. both 42-kda and 45-kda protein preparations similarly bound to human igg1 to igg4, human ig ... | 1990 | 2332638 |
| human carboxypeptidase e. isolation and characterization of the cdna, sequence conservation, expression and processing in vitro. | carboxypeptidase e (cpe), which cleaves c-terminal amino acid residues and is involved in neuropeptide processing, is itself subject to intracellular processing. human cpe cdna was isolated and sequence comparisons were made with those of a previously isolated brain cdna (m1622) encoding rat cpe and of other human carboxypeptidases (m and n). human (2.5 kb) and rat (2.1 kb) cpe cdnas approximated to the size of their respective mrnas; additional sequences were located in putative 5' and 3' untra ... | 1990 | 2334405 |
| nitrate biosynthesis in rats, ferrets and humans. precursor studies with l-arginine. | l-arginine, the primary nitrogen source for nitric oxide synthesized by many cell types in culture and for biosynthesized nitrate in humans, is also a nitrogen source for biosynthesized nitrate in rats and ferrets. after administration of [15n2]l-arginine to rats and ferrets, [15n]no3- was detected in urine. escherichia coli lipopolysaccharide induced more than a 10-fold increase in urinary nitrate in rats and a parallel increase in incorporation of 15n from [15n2]l-arginine into no3-. bradykini ... | 1990 | 2335012 |
| isolation of cdna coding for the major mite allergen der p ii by ige plaque immunoassay. | a lambda gt11 library made with cdna from the house dust mite dermatophagoides pteronyssinus was screened with human allergic serum by ige plaque radioimmunoassay. this resulted in the isolation of clones coding for the major allergen der p ii. the cdna coded for a 129-residue protein of 14,131 daltons with no n-glycosylation sites. no sequence homology with other proteins was evident. the der p ii expressed in escherichia coli reacted with ige in 14 of 17 sera from mite-allergic patients giving ... | 1990 | 2341191 |
| purification and cloning of interferon-stimulated gene factor 2 (isgf2): isgf2 (irf-1) can bind to the promoters of both beta interferon- and interferon-stimulated genes but is not a primary transcriptional activator of either. | interferon-stimulated gene factor 2 (isgf2) was purified from hela cells treated with alpha interferon. the factor, a single polypeptide of 56 kilodaltons (kda), bound both to the central 9 base pairs of the 15-base-pair interferon-stimulated response element (isre) that is required for transcriptional activation of interferon-stimulated genes and to the prd-i regulatory element of the beta interferon gene. isgf2 was a phosphoprotein, and dephosphorylation in vitro reduced its dna-binding activi ... | 1990 | 2342456 |
| cloning and expression of a cdna encoding human placental protein 11, a putative serine protease with diagnostic significance as a tumor marker. | the placental protein 11 (pp11) can act as a tumor marker because of its specific association with various forms of cancer. a lambda gt11 cdna library prepared from human placenta was screened with a polyclonal anti-pp11 antiserum. out of 10(6) independent clones, only one clone reacted with the anti-pp11 antiserum. the isolated cdna coded only for the carboxy-terminal part of pp11 and was subsequently used to rescreen a lambda gt10 placental cdna library. two cdna clones out of 10(6) screened w ... | 1990 | 2350438 |
| 27 amino acid residues can be deleted from the n-terminus of human lymphotoxin without impairment of its cytotoxic activity. | in order to study the relationship between activity and structure of human lymphotoxin (hlt, 171 aa), we synthesized the gene (519 bp) for hlt and expressed it in escherichia coli. purification of the recombinant hlt from crude extracts was difficult because of the low level of expression of the gene. to improve the yield of the recombinant protein, we prepared five truncated genes for mutant proteins in which 25, 26, 27, 28 and 37 amino acid residues, respectively, were missing from the n-termi ... | 1990 | 2361063 |
| ocular inflammatory effects of intravitreally injected tumor necrosis factor-alpha and endotoxin. | intravitreal injection of human recombinant tumor necrosis factor-alpha (tnf) induced inflammation in the rabbit eye characterized by dilation of blood vessels in the iris, disruption of the blood-ocular barriers, infiltration of inflammatory cells into the anterior chamber, and accumulation of prostaglandin e in intraocular fluids. inflammation first appeared on day 1, increased on day 2, and remained elevated on day 7. the inflammatory cell infiltrate in the anterior segment of the eye was lar ... | 1990 | 2361736 |
| in vivo tumor targeting of a recombinant single-chain antigen-binding protein. | we describe here the first in vivo targeting of tumors with a single-chain antigen-binding protein. the molecule, which was constructed and expressed in escherichia coli, is a novel recombinant protein composed of a variable light-chain (vl), amino acid sequence of an immunoglobulin tethered to a variable heavy-chain (vh) sequence by a designed peptide. we show that this protein, derived from the dna sequence of the variable regions of the antitumor monoclonal antibody b6.2, has the same in vitr ... | 1990 | 2362290 |
| the 75-kilodalton cytoplasmic chlamydia trachomatis l2 polypeptide is a dnak-like protein. | the gene coding for the 75-kilodalton cytoplasmic chlamydia trachomatis l2 polypeptide has been cloned in escherichia coli, and the nucleotide sequence has been determined. the cloned dna fragment contained the coding region as well as the putative promoter. the deduced amino acid sequence of the 1,980-base-pair open reading frame revealed 94% homology with a 75-kilodalton protein from c. trachomatis serovar d and 57% homology with the dnak proteins of e. coli and of bacillus megaterium, while a ... | 1990 | 2365454 |
| characterization of borrelia burgdorferi proteins reactive with antibodies in synovial fluid of a patient with lyme arthritis. | four borrelia burgdorferi proteins reactive with antibodies in the synovial fluid of a patient with lyme arthritis were characterized. homology between amino acid sequences of immunoreactive spirochetal proteins and human proteins, including members of the escherichia coli groel protein family, suggests that antigenic mimicry may play a role in the pathogenesis of lyme arthritis. | 1990 | 2365463 |
| tumor necrosis factor-alpha production of influenza a virus-infected macrophages and potentiating effect of lipopolysaccharides. | influenza a virus infections are commonly associated with symptoms that suggest involvement of tnf-alpha. in this study, we exposed human monocytes, rat alveolar macrophages, and murine pu5-1.8 macrophages to influenza a virus, strain puerto rico 8. we observed a productive infection that was accompanied by tnf-alpha mrna accumulation, tnf-alpha release and subsequent cell death. tnf-alpha production was dependent on exposure to live virus, in contrast to ifn release that was also induced by uv- ... | 1990 | 2391423 |
| development of micrometastases: earliest events detected with bacterial lacz gene-tagged tumor cells. | for the study of micrometastases at their earliest stages, we transfected the lacz gene, which codes for beta-d-galactosidase in escherichia coli, into balb/c 3t3 cells transformed by the ha-ras oncogene (also known as hras1) of a human ej bladder carcinoma. these cells were subsequently injected into 6-week-old, female athymic ncr-nu nude mice by several routes. with chromogenic detection of the product of the lacz gene (a heterologous gene not observed in animal cells) by use of 5-bromo-4-chlo ... | 1990 | 2391720 |
| deacylation of structurally diverse lipopolysaccharides by human acyloxyacyl hydrolase. | acyloxyacyl hydrolase, a leukocyte enzyme previously has been shown to catalyze the hydrolysis of secondary (acyloxyacyl-linked) fatty acyl chains from the nonreducing glucosamine of the lipid a region of rough salmonella typhimurium lipopolysaccharide (lps). we describe here the activity of this enzyme toward smooth s. typhimurium lps and lps from escherichia coli, pseudomonas aeruginosa, haemophilus influenzae, neisseria meningitidis, and neisseria gonorrhoeae. acyloxyacyl hydrolase released t ... | 1990 | 2398058 |
| detoxification of plasma containing lipopolysaccharide by adsorption. | we compared the ability of 13 materials, all with known affinity for lipopolysaccharide (lps), to remove lps from water, pooled normal human plasma, and plasma from a patient with gram-negative bacterial sepsis. escherichia coli o111:b4 lps was added to water and pooled normal human plasma to a concentration of 200 ng/ml and aliquots were adsorbed in parallel with each of the materials. plasma containing 25 ng/ml of lps was obtained by plasmapheresis from a patient with fatal klebsiella pneumoni ... | 1990 | 2403508 |
| isolation and structural characterization of a cdna clone encoding the human dna repair protein for o6-alkylguanine. | o6-methylguanine-dna methyltransferase (mgmt; dna-o6-methylguanine:protein-l-cysteine s-methyltransferase, ec 2.1.1.63), a unique dna repair protein present in most organisms, removes the carcinogenic and mutagenic adduct o6-alkylguanine from dna by stoichiometrically accepting the alkyl group on a cysteine residue in a suicide reaction. the mammalian protein is highly regulated in both somatic and germ-line cells. in addition, the toxicity of certain alkylating drugs in tumor and normal cells i ... | 1990 | 2405387 |
| expression of human parathyroid hormone in escherichia coli. | human parathyroid hormone (hpth) is a peptide hormone consisting of 84 amino acids. using the expression plasmid pkk223-3 with the strong tacpromoter, we have produced a variant of hpth in e. coli. from the expression plasmid construct the expected product was hpth with an n-terminal extension of met-gly. the peptide was extracted from e. coli cells and purified by high performance liquid chromatography. in two different gel electrophoresis systems including identification by immunoblotting the ... | 1990 | 2405851 |
| complete nucleotide sequence of the streptococcal c5a peptidase gene of streptococcus pyogenes. | streptococcal c5a peptidase (scp), a recently discovered virulence factor of streptococcus pyogenes, specifically cleaves the human serum chemotaxin c5a near its carboxyl terminus, destroying its ability to serve as a chemoattractant. we previously localized the scp gene, scpa, to the 5.8-kb insert of the recombinant plasmid ptt1. here we present the complete nucleotide sequence of scpa and its flanking regions. the gene initiates at a ttg codon and consists of 3501 base pairs, specifying a prec ... | 1990 | 2406246 |
| specificity and inhibitory activity of antibodies to plasmodium falciparum aldolase. | the multiplication of plasmodium falciparum within rbc is energy-dependent and the glucose consumption of infected rbc is increased more than 50 times over the consumption of normal rbc. high levels of glycolytic enzymes such as fructose-1,6-diphosphate aldolase (p41) have been detected in infected rbc. expression of the cloned aldolase gene of p. falciparum in escherichia coli resulted in an enzymatically active polypeptide with a high sp. act. and the recombinant p41 aldolase was used for enzy ... | 1990 | 2406342 |
| are many z-dna binding proteins actually phospholipid-binding proteins? | we used a z-dna affinity column to isolate a collection of z-dna binding proteins from a high salt extract of escherichia coli. we identified one of the major z-dna binding proteins of this fraction, not as a protein involved in gene regulation or genetic recombination, but rather as an outer membrane porin protein. we then showed that several other known phospholipid-binding proteins (bovine lung annexins and human serum lipoproteins) also bind much more tightly to z-dna than to b-dna. in all c ... | 1990 | 2406717 |
| generation of deletion derivatives by targeted transformation of human-derived yeast artificial chromosomes. | mammalian dna segments cloned as yeast artificial chromosomes (yacs) can be manipulated by dna-mediated transformation when placed in an appropriate yeast genetic background. a "fragmenting vector" has been developed that can introduce a yeast telomere and selectable marker into human-derived yacs at specific sites by means of homologous recombination, deleting all sequences distal to the recombination site. a powerful application of the method uses a human alu family repeat sequence to target r ... | 1990 | 2406718 |
| protein n-myristoylation in escherichia coli: reconstitution of a eukaryotic protein modification in bacteria. | protein n-myristoylation refers to the covalent attachment of a myristoyl group (c14:0), via amide linkage, to the nh2-terminal glycine residue of certain cellular and viral proteins. myristoyl-coa:protein n-myristoyltransferase (nmt) catalyzes this cotranslational modification. we have developed a system for studying the substrate requirements and biological effects of protein n-myristoylation as well as nmt structure-activity relationships. expression of the yeast nmt1 gene in escherichia coli ... | 1990 | 2406721 |
| high level, regulated expression of the chimeric p-enolpyruvate carboxykinase (gtp)-bacterial o6-alkylguanine-dna alkyltransferase (ada) gene in transgenic mice. | transgenic animals expressing genes capable of repairing dna may be a valuable tool to study the effect of dna-damaging agents on tissue-specific carcinogenesis. for this reason, we constructed a chimeric gene consisting of the promoter-regulatory region of the phosphoenolpyruvate carboxykinase (gtp) (ec 4.1.1.32) (pepck) gene linked to the escherichia coli ada gene coding for o6-alkylguanine-dna alkyltransferase and the polyadenylate region from the bovine growth hormone gene. the pepck promote ... | 1990 | 2407342 |
| complementation of the xeroderma pigmentosum dna repair synthesis defect with escherichia coli uvrabc proteins in a cell-free system. | a newly developed cell-free system was used to study dna repair synthesis carried out by extracts from human cell lines in vitro. extracts from a normal human lymphoid cell line and from cell lines established from individuals with hereditary dysplastic nevus syndrome perform damage-dependent repair synthesis in plasmid dna treated with cis- or trans-diamminedichloro-platinum(ii) or irradiated with ultraviolet light. cell extracts of xeroderma pigmentosum origin (complementation groups a, c, d, ... | 1990 | 2408009 |
| modular mutagenesis of human placental ribonuclease inhibitor, a protein with leucine-rich repeats. | human placental ribonuclease inhibitor (pri) is a potent protein inhibitor of pancreatic ribonucleases and the homologous blood vessel-inducing protein angiogenin. although inhibition by pri occurs with a 1:1 stoichiometry, its primary structure is composed predominantly of seven internal leucine-rich repeats. these internal repeats were systematically deleted either singly or in combination by "modular" mutagenesis. deletion of repeat units 3 plus 4 or repeat unit 6 results in mutants that both ... | 1990 | 2408043 |
| comparison of biological and immunological activities of human monocyte-derived interleukin 1 beta and human recombinant interleukin 1 beta. | recombinant human interleukin 1 beta (rhil-1 beta) and supernatants of escherichia coli lipopolysaccharides-stimulated human monocyte (mo) cultures, containing native human il-1 beta (nhil-1 beta), demonstrate significant differences when tested in the mouse co-stimulatory thymocyte (lymphocyte activating factor [laf]) assay. the aims of the present study were to investigate this characteristic difference between rhil-1 beta and mo culture supernatants (mo supernatants), and to compare the biolo ... | 1990 | 2408138 |
| slo4, a new interferon inducer isolated from klebsiella pneumoniae and escherichia coli. | a product isolated from klebsiella pneumoniae and escherichia coli, coded slo4, has been shown to be effective in endogenous interferon induction in vivo in mouse when administered ip or iv, and in vitro with human leukocyte cultures. in these two systems induced interferon was defined. the inducer has not yet been characterized but seems not to belong to any components known to be interferon inducers such as viral particles, nucleic acids or endotoxins. an analytical study will be carried out t ... | 1985 | 2409177 |
| deposition of c3b and ic3b onto particulate activators of the human complement system. quantitation with monoclonal antibodies to human c3. | monoclonal antibodies were used to determine the number and molecular form of c3 bound to particulate activators of the complement (c) system by human serum. sheep erythrocytes (e) coated with igm (eigm) and igg (eigg) were used to study activation of the classical pathway (cp). yeast (y), rabbit erythrocytes (er), and five species of bacteria (escherichia coli, staphylococcus aureus, streptococcus pneumoniae type 3, streptococcus pyogenes, and hemophilus influenzae type b) were used to study ac ... | 1985 | 2409200 |
| structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes. | we have determined the sequence coding for human alpha 1-acid glycoprotein from two independently isolated cdna clones and a genomic clone. the aminoacid sequences deduced from the three clones, deriving from three different individuals, are identical. southern blot analysis on human dna indicates that there are at least two genes coding for alpha 1-agp. we propose that alpha 1-agp found in plasma is a mixture of the products of these two different genes. this is the simpler explanation for the ... | 1985 | 2409529 |
| inhibition of mast cell sensitization in vitro by a human immunoglobulin epsilon-chain fragment synthesized in escherichia coli. | an immunoglobulin epsilon-chain fragment was synthesized in e. coli by cloning and expression of the gene coding for the second, third and fourth constant domains of the human ige heavy chain. the bacterial ch2-4 polypeptide product was assembled by oxidation into a covalently linked dimeric epsilon-chain molecule presumably analogous to the fc region of native ige. this bacterial fc epsilon preparation, within the concentration range 0.01-10 micrograms/ml, inhibited sensitization of human lung ... | 1985 | 2412840 |
| bacterial adherence and hemolysin production from escherichia coli induces histamine and leukotriene release from various cells. | we investigated the role of bacterial adherence and hemolysin production from escherichia coli parent and genetically cloned strains as to their effects on histamine release from rat mast cells and leukotriene generation from human polymorphonuclear granulocytes. these mediators were involved in the induction of inflammatory disease processes and led, for example, to enhancement of vascular permeability, chemotaxis (leukotriene b4 [ltb4]), chemoaggregation, lysosomal enzyme release, and smooth m ... | 1985 | 2412960 |
| characterization of enterotoxigenic escherichia coli. serotypes, enterotoxins, adhesion fimbriae, and the presence of plasmids. | enterotoxigenic escherichia coli of human and procine origin were characterized with respect to their o and h antigens, fimbrial antigens, and type of enterotoxin produced. enterotoxin production was determined by bioassay (infant mice) and enzyme-linked immunoassay (elisa). the presence of genes coding for the enterotoxins was determined by dna-dna hybridization. the number and molecular size of plasmids in the enterotoxigenic strains were determined by gel electrophoresis. strains with the sam ... | 1985 | 2413710 |
| reconstitution of rnaase p activity using inactive subunits from e. coli and hela cells. | hela cell rnaase p activity found in the flow-through of anti-sm affinity columns can be separated into inactive rna and protein components. these components can be used to reconstitute active hybrid enzyme complexes with purified subunits from e. coli rnaase p. the rna in the hela cell fractions employed is enriched for species between 85 and 115 nucleotides long. this reconstitution assay is a convenient means of purifying the functional rna and protein of hela cell rnaase p. probes derived fr ... | 1986 | 2417727 |
| chemiluminescence response of the human polymorphonuclear neutrophil to lipopolysaccharides. | polymorphonuclear neutrophils (pmn) respond to a variety of stimuli with a sequence of reactions that lead to the production of "active oxygen" species, including h2o2, free radicals, such as superoxide (o2-.) and hydroxyl (ho.), and singlet molecular oxygen (1o2). some of these can oxidize (5-amino-2,3-dihydrophthalazine 1,4-dione) (luminol) to the ground state aminophthalate ion; this reaction sequence is accompanied by the generation of a photon and forms the basis for the chemiluminescence ( ... | 1985 | 2420454 |
| [mechanism of action of quinolones]. | how do the quinolones inhibit bacteria? the chromosome of bacteria is composed of helical double-stranded dna and contains 60 to 70 spatial regions of organisation, termed domains of supercoiling. each domain is about 20 mu long, attached to an rna core and is organised by supercoiling which occurs quite independently of the dna coiling in any other domain. supercoiling is controlled by the enzyme dna gyrase, which introduces transient breaks into both dna strands of each domain, removes about 4 ... | 1986 | 2420724 |
| the human 18s ribosomal rna gene: evolution and stability. | we report the 1,870-base-pair primary sequence of a human 18s rrna gene and propose a secondary structure based on this sequence and the general mammalian structure. a basic secondary structure for the small subunit rrna has been preserved throughout evolution by compensatory and neutral base changes in double-stranded regions. the molecule contains eight regions that can vary in structure and that comprise 432 bases, while 1,438 bases belong to regions of conserved structure among all species t ... | 1986 | 2422931 |
| influence of bacterial endotoxins on basophil histamine release. potentiation of antigen- and bacteria-induced histamine release. | the histamine-releasing capability of lipopolysaccharides (lps) was examined in human leukocyte suspensions. lps alone did not release histamine, but was found to enhance the histamine release caused by anti-ige. also the ige-mediated histamine release caused by specific antigens (allergens or bacteria) in sensitized individuals was enhanced by lps. the potentiating effect of lps was observed in grass pollen and dog dander allergic patients as well as in patients sensitized to e. coli or staph. ... | 1986 | 2422974 |
| are cross-reacting natural antibodies multispecific? | human natural antibodies to antigens of escherichia coli and serratia marcescens were studied for cross-reactivity. the organisms were grown on synthetic media and extracted at 100 degrees c. the extracts were precipitated three times at 71% ethanol concentration and redissolved at the desired concentration. these preparations were referred to as escherichia antigen (ea) and serratia antigen (sa). they readily coated red blood cells (rbc), which then could be used for passive agglutination tests ... | 1986 | 2423463 |
| [new data on the biological action of normal immunoglobulins]. | experiments on 2,520 cba mice (cba x x c57bl) f1 nice have shown that the injection of homologous serum immunoglobulins (obtained from intact and blood-stimulated animals), made 2 hours after gamma irradiation from a 60co source, prevents the development of intestinal dysbacteriosis and endogenous infection. the injection of mouse and human immunoglobulins to nonirradiated mice improved their resistance to experimental infection with escherichia coli live culture, increased the expression of rec ... | 1986 | 2425516 |
| use of a portable ribosome-binding site for maximizing expression of a eukaryotic gene in escherichia coli. | to maximize expression of a eukaryotic gene in escherichia coli, a series of plasmids were constructed containing various synthetic ribosome-binding sites (rbs). these sites consist of a shine-dalgarno (sd) region (with translation stop codons in all three reading frames) positioned at distances 5-9 nucleotides (nt) from the aug initiator codon of the gene coding for human t-cell growth factor (tcgf or il-2). the region encompassing the rbs through the tcgf structural gene from each of these pla ... | 1986 | 2426157 |
| expression of reverse transcriptase activity of human t-lymphotropic virus type iii (htlv-iii/lav) in escherichia coli. | the pol gene from a biologically active clone of the human t-cell lymphotropic virus type iii provirus was inserted into a bacterial expression vector. the resulting gene fusion induced the formation of active reverse transcriptase that could be readily detected in extracts of bacterial cells. the activity exhibited the template and divalent cation requirements of the authentic enzyme. these constructs will be useful for safe and rapid analysis of potential inhibitors of this important enzyme. | 1986 | 2426471 |
| polymyositis and molecular mimicry, a mechanism of autoimmunity. | the amino acid sequences of escherichia coli histidyl-trna synthetase and alanyl-trna synthetase, two proteins recently identified as autoantigens in polymyositis, were compared by a computer alignment procedure with those of the 3600 proteins tabulated in the national biomedical research foundation protein sequence database. both proteins contain sequences long enough to function as epitopes that match sequences on viral and muscle proteins. the homology thus revealed not only lends strong supp ... | 1986 | 2427902 |
| use of the phage lambda pl promoter for high-level expression of human interferons in escherichia coli. | | 1986 | 2429151 |
| monoclonal antibodies against escherichia coli heat-stable toxin (sta) and their use in a diagnostic st ganglioside gm1-enzyme-linked immunosorbent assay. | seven monoclonal antibodies (mabs) against heat-stable enterotoxin (st) from a human escherichia coli isolate were prepared and evaluated for their usefulness in an st immunodetection assay, the st ganglioside gm1-enzyme-linked immunosorbent assay (elisa). this assay is based on the ability of sta, as present in, for example, culture filtrates from st-producing e. coli, to inhibit specific anti-st antibody from binding to solid-phase-bound st ganglioside (gm1-bound st-cholera b subunit). four of ... | 1986 | 2429984 |
| the l2 open reading frame of human papillomavirus type 1a encodes a minor structural protein carrying type-specific antigens. | the proteins encoded by the open reading frames of papillomavirus genomes and the minor polypeptides detected in purified virions are still poorly defined. we show here by its expression in escherichia coli that the open reading frame l2 of human papillomavirus type 1a codes for a minor structural protein of mr 76,000. antisera raised against a truncated l2-beta-galactosidase fusion protein in which the conserved n-terminal region of l2 is missing are type specific for human papillomavirus type ... | 1986 | 2430112 |
| endothelial plasminogen activator inhibitor (pai): a new member of the serpin gene family. | a human endothelial cdna expression library, based on the escherichia coli plasmid puc9, was screened with a heterologous antibody raised against purified bovine aortic endothelial plasminogen activator inhibitor (pai). a synthetic oligonucleotide, derived from a partial pai cdna expression clone, was used to select a full-length pai cdna, the size of which coincides with the length of pai mrna (approximately 2350 nucleotides) as determined by northern blot analysis. the authenticity of full-len ... | 1986 | 2430793 |
| [preparation and characterization of specific antisera directed against different polypeptide domains encoded by the c-myc oncogene for studying the expression of this gene introduced into quail or rat cells]. | by using bacterial expression vectors, we have prepared antisera directed against two polypeptidic domains encoded by exons 2 and 3 of the human c-myc oncogene. these antisera which detect specifically the human c-myc proteins allow us to analyse the expression of human c-myc gene activated by retroviral sequences and introduced in quail embryo cells (qec) or in established rat embryo fibroblastic cell line (208 f). although human myc mrna are expressed in the two cell types, the p64/p67 human c ... | 1986 | 2433006 |
| human t cell clones recognize two abundant mycobacterium tuberculosis protein antigens expressed in escherichia coli. | human t cells reactive to mycobacterium tuberculosis were cloned from peripheral blood mononuclear cells (pbmc) of four tuberculosis patients by using whole irradiated bacilli as the in vitro stimulatory agent. twenty-two t cell clones (cd4+) were tested for their reactivity to 12 different mycobacterial species and showed a distribution from limited to broad cross-reactivity. these t cell clones were also tested for their reactivity to three abundant m. tuberculosis proteins of 65, 19, and 14 k ... | 1987 | 2433335 |
| a neutralizing epitope on human rhinovirus type 2 includes amino acid residues between 153 and 164 of virus capsid protein vp2. | use has been made of a monoclonal antibody (designated 8f5) to map a neutralizing epitope on the viral capsid protein vp2 of human rhinovirus 2 (hrv2). this antibody which was raised against the native virus, neutralizes hrv2 and is also capable of recognizing denatured vp2 on western blots. to examine the binding site of 8f5, vp2 of hrv2 was expressed in escherichia coli. deletions starting at the 3' end were then introduced into the gene for vp2 using bal-31 nuclease. polypeptides shortened at ... | 1987 | 2434607 |
| synthetic gene construct expressing a repeated and highly immunogenic epitope of the plasmodium falciparum antigen pf155. | the plasmodium falciparum-derived antigen pf155 contains two blocks of tandemly repeated amino acid sequences. a pair of complementary oligonucleotides, encoding the c-terminally located repeat val-glu-his-asp-ala-glu-glu-asn, were synthesized. the oligonucleotides were polymerized by ligation, and the resulting multimers were cloned into an expression vector. one construct that contained four copies of the repeat was expressed in escherichia coli. the product, a fusion protein, was soluble and ... | 1987 | 2434955 |
| structure-activity relationship of gramicidin s analogues on membrane permeability. | the previous study of the action of gramicidin s on bacteria (katsu, t., kobayashi, h. and fujita, y. (1986) biochim. biophys. acta 860, 608-619) prompted us to investigate further the structure-activity relationship of the gramicidin s analogues on membrane permeability. two types of the gramicidin s analogues were used in the present study: (1) cyclo(-x-d-leu-d-lys-d-leu-l-pro-)2, where x = gly, d-leu and d-cyclohexylalanine (d-chxala); (2) n,n'-diacetyl derivative of gramicidin s (diacetyl-gr ... | 1987 | 2437956 |
| localization of the cellular-fibronectin-specific epitope recognized by the monoclonal antibody ist-9 using fusion proteins expressed in e. coli. | here we report on a monoclonal antibody (ist-9) which distinguishes between human cellular and plasma fibronectin. using beta-galactosidase-fibronectin fusion proteins expressed in e. coli we have demonstrated that this monoclonal antibody is specific for a fibronectin segment (ed) which can be included or omitted from the molecule depending on the pattern of splicing of the mrna precursors. furthermore, using the same fusion proteins we have been able to localize precisely the epitopes of two o ... | 1987 | 2438158 |
| [combined effects of astromicin and human gamma-globulin prepared for intravenous use]. | combination therapy of antibiotics and human intravenous gamma immunoglobulin (igg) prepared for intravenous use is widely applied to control severe infectious diseases. to evaluate the antimicrobial activity of astromicin (astm, fortimicin), the combination effect of astm with igg was investigated in vitro and in mice with experimental infection (i.p.) with pseudomonas aeruginosa bmh no. 1. sulfonated igg (venilon, vl), and plasmin-treated igg (venoglobulin, vg) were administered (i.v., 42 mg/k ... | 1987 | 2439721 |
| specialized ribosome system: preferential translation of a single mrna species by a subpopulation of mutated ribosomes in escherichia coli. | in escherichia coli, all mrnas are translated by one pool of functionally identical ribosomes. here, we describe a system in which a subpopulation of modified ribosomes are directed to a single mutated mrna species. this was accomplished by changing the shine-dalgarno sequence that precedes the heterologous human growth hormone gene from 5' ggagg to 5' cctcc or 5' gtgtg. translation of these modified mrnas by wild-type ribosomes is very inefficient. when the anti-shine-dalgarno region (i.e., the ... | 1987 | 2440028 |
| construction of expression vectors for the production of interferons in yeast. | expression vectors designed for the production of foreign proteins in yeast are constructed as a composite of functional dna segments brought together in a single plasmid dna molecule. such a composite dna molecule constitutes a complex entity in terms of its replication and selection functions (in both e. coli and s. cerevisiae) and its gene expression properties. the latter depend critically upon transcriptional control signals (the promoter and terminator regions from a highly expressed yeast ... | 1987 | 2440740 |
| specific binding of the human s protein (vitronectin) to streptococci, staphylococcus aureus, and escherichia coli. | specific binding of the 125i-labeled human s protein (vitronectin) which has been shown to be identical with serum-spreading factor, was observed with group a, c, and g streptococci as well as with staphylococcus aureus and escherichia coli. the specific binding of s protein to group a, c, and g streptococci was high, whereas the binding to s. aureus and e. coli cultures was moderate. in contrast, group b streptococci and a number of other bacterial species tested did not interact with s protein ... | 1987 | 2440809 |
| c1-esterase inhibitor in early septicemia. | in bacteremic pigs, both administered human c1inh and endogenous c1inh lost inhibitory activity. in septic pigs which received human c1inh a difference between immunologically determined concentration and inhibitory activity of c1inh became apparent. in animals which survived at least 24 hrs the c1inh activity showed an acute phase response. in a model of e. coli bacteremia in weaned pigs administration of human c1inh showed protective effects on lung capillary permeability as well as less leuco ... | 1987 | 2441404 |
| pilus-mediated interactions of the escherichia coli strain rdec-1 with mucosal glycoproteins in the small intestine of rabbits. | escherichia coli strain rdec-1 (serotype 015:nm) is an effacing adherent enteropathogen that binds to the intestine of rabbits in a manner morphologically identical to the binding of human enteropathogenic e. coli strains to human intestine. the rabbit enteropathogen adheres to mucosal enterocytes in vivo and to microvillus membranes in vitro. binding of rdec-1 to ileal brush borders and to m cells overlying peyer's patches is mediated by pili (fimbriae) expressed on the cell surface of bacteria ... | 1987 | 2442061 |
| antigenic determinants of the cholera/coli family of enterotoxins. | hybridoma-derived monoclonal antibodies were raised to enterotoxins of the cholera family and to chimeric b-subunit proteins in which individual amino acid residues of a heat-labile, cholera-related enterotoxin from an escherichia coli strain of porcine origin (p-lt) were substituted with corresponding residues from such an enterotoxin from an e. coli strain of human origin (h-lt). single amino acid substitutions were found to have profound effects on the physicochemical behavior of the proteins ... | 1987 | 2446368 |
| aids virus reverse transcriptase defined by high level expression in escherichia coli. | the causative agent of aids the human immunodeficiency virus (hiv) encodes as part of its pol gene a reverse transcriptase (rt) which has a key role in the replication of the virus and thus constitutes an ideal target for antiviral chemotherapy. the purified hiv rt from virus particles consists of two related polypeptides of 66 and 51 kd mol. wt and similar polypeptides are found on expression of the complete hiv pol gene using prokaryotic systems. here we describe the expression of the 66-kd pr ... | 1987 | 2446866 |
| incorporation of bacterial lipopolysaccharide by human leu-11a+ natural killer cells. ultrastructural and functional correlations. | lipopolysaccharides (lps) of gram-negative bacteria are known to augment the ability of macrophages and natural killer (nk) cells to lyse susceptible target cells. in the present studies, we sought correlations between the ultrastructural changes and function of leu-11a+ cells from the peripheral blood mononuclear cells which occurred as a result of their incorporation of lps. we also studied the effect of lps on the nk activity of purified leu-11a+ cells. lps samples from e. coli and pseudomona ... | 1988 | 2448547 |
| [shigella toxicity in immunological tolerance]. | the toxic effect of killed and live shigella sonnei cultures on normal mice and on mice, tolerant to shigella o-antigen and to human erythrocytes of different blood groups (in the abo system) was under study. the toxicity of shigellae, introduced intraperitoneally, has been found to depend on their viability, on their capacity for penetration into the blood, and on the split character of immunological tolerance to shigella antigens. | 1987 | 2448979 |