| phylogenetic analysis of prevotella nigrescens, prevotella intermedia and porphyromonas gingivalis clinical strains reveals a clear species clustering. | prevotella nigrescens, prevotella intermedia and porphyromonas gingivalis are oral pathogens from the family bacteroidaceae, regularly isolated from cases of gingivitis and periodontitis. in this study, the phylogenetic variability of these three bacterial species was investigated by means of 16s rrna (rrs) gene sequence comparisons of a set of epidemiologically and geographically diverse isolates. for each of the three species, the rrs gene sequences of 11 clinical isolates as well as the corre ... | 2002 | 12148655 |
| [detection of periodontal pathogenic microorganisms in atheromatous plaque. preliminary results]. | recent studies suggest that chronic infections, including those associated with periodontitis, increase the risk for coronary vascular disease. we hypothesize that oral microorganisms including periodontal bacterial pathogens enter the blood stream during transient bacteremias where they may play a role in the development and progression of atherosclerosis. | 2002 | 12149943 |
| contamination of interdental brushes by periodontopathogens. | experimental studies have shown that intraoral transmission of bacteria can occur. of course, the question arises as to how this transmission may happen. in this study, the contamination of interdental brushes by periodontopathogens is examined and compared to the microbial load of the periodontal pockets. in ten untreated chronic periodontitis patients, four interdental sites were professionally brushed with one interdental brush per patient. subsequently, samples from the depths of the pockets ... | 2002 | 12166717 |
| matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and proteomics: a new era in anaerobic microbiology. | genome sequence data provide a framework for predicting potential microbial activities; however, the proteome content of the cell dictates its response to its environment. microbiology is witnessing a major initiative to elucidate the nature of the proteome of large numbers of species. the tool driving the proteomic revolution is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. during the analysis process, proteins are ionized and separated on the basis of their mass ... | 2002 | 12173110 |
| microbiological findings and host response in patients with peri-implantitis. | the aim of the present study was to characterise microbiota and inflammatory host response around implants and teeth in patients with peri-implantitis. we included 17 partly edentulous patients with a total of 98 implants, of which 45 showed marginal bone loss of more than three fixture threads after the first year of loading. nineteen subjects with stable marginal tissue conditions served as controls. oral hygiene, gingival inflammation, and probing pocket depth were evaluated clinically at tee ... | 2002 | 12175371 |
| two-stage imz implants and iti implants inserted in a single-stage procedure. a prospective comparative study. | the aim of this study was to evaluate the feasibility of using a two-stage implant system in a single-stage procedure and to study the impact of the microgap at crestal level and to monitor the microflora in the peri-implant area. forty edentulous patients (cawood & howell class v-vi) participated in this study. after randomisation, 20 patients received two imz implants inserted in a single-stage procedure and 20 patients received two iti implants. after 3 months, overdentures were fabricated, s ... | 2002 | 12175374 |
| antimicrobial effectiveness of electro-chemically activated water as an endodontic irrigation solution. | the aim of this in vitro study was to evaluate the antimicrobial effectiveness of electro-chemically activated water (eca) as an endodontic irrigation solution. | 2001 | 12193270 |
| combinations of bacterial species in endodontic infections. | this study was undertaken to investigate combinations of bacteria found in root-canal infections of teeth with periapical bone destruction without clinical signs and symptoms. | 2002 | 12196223 |
| microbiological, immunological and genetic factors in family members with periodontitis as a manifestation of systemic disease, associated with hematological disorders. | the microflora, immunological profiles of host defence functions, and human leukocyte antigen (hla) findings are reported for a mother, son and daughter who were diagnosed as having 'periodontitis as a manifestation of systemic diseases, associated with hematological disorders'. examinations were made of the bacterial flora from the periodontal pocket, neutrophil chemotaxis, neutrophil phagocytosis, and the genotypes (dqb1) and serotypes (dr locus) of hla class ii antigens. phenotypic analyses o ... | 2002 | 12200976 |
| prevotella intermedia native plasmid can be mobilized by an escherichia coli conjugal incp plasmid. | we have determined the nucleotide sequence of a small prevotella intermedia cryptic plasmid, pyhbi1, which consisted of sequences that were highly homologous to the amino acid sequence of the replication and mobilization proteins found in related organisms. we have also demonstrated that chimeric plasmids derived from this p. intermedia native plasmid can be mobilized between escherichia coli strains by using a broad-host-range e. coli conjugative plasmid, incp plasmid rp4. the results suggest t ... | 2002 | 12206757 |
| frequency of reactivity for porphyromonas gingivalis and prevotella spp. in supra- and subgingival plaques, and periodontal clinical parameters according to subject age. | the present study was conducted to assess the association between selected clinical parameters and the distribution of porphyromonas gingivalis (pg), prevotella intermedia (pi), prevotella nigrescens (pn), and prevotella melaninogenica (pm) in supra- and subgingival plaque samples measured by an immunoslot blot assay (iba) using their monoclonal antibodies. | 2002 | 12211497 |
| gtr treatment of intrabony defects in patients with early-onset and chronic adult periodontitis. | young, systemically healthy subjects may suffer from early-onset forms of periodontitis characterized by the presence of localized deep vertical bony defects. the aim of this study was to compare the healing response after guided tissue regeneration (gtr) treatment of similar intrabony defects in patients affected by early-onset and chronic adult periodontitis. twenty systemically healthy, nonsmoking subjects were enrolled in the study; 10 were affected by early-onset periodontitis (eop) and 10 ... | 2002 | 12212679 |
| [an in vitro study of the action of kola nitida on bacterial strains implicated in dental caries and periodontal diseases]. | the nitida kola is a substance extracted from the kolanut. in west africa its use by chewing is widespread among the manding people. it's said to have tonic, stimulant and aphrodisiac characteristics and even recent studies have shown that it has antibacterial characteristics. the aim of this study is to make an estimation of the nitida kola's effects on different bacterial species involved in the two main oral and dental pathologies (teeth decays and periodontal illnesses). the obtained results ... | 2002 | 12221807 |
| biochemical-genetic analysis and distribution of des-1, an ambler class a extended-spectrum beta-lactamase from desulfovibrio desulfuricans. | desulfovibrio spp. are gram-negative anaerobes phylogenetically related to bacteroides spp., which are rarely isolated and which are mostly isolated from intra-abdominal abscesses. desulfovibrio desulfuricans clinical isolate d3 had a clavulanic acid-inhibited beta-lactam resistance profile and was resistant to some expanded-spectrum cephalosporins. a beta-lactamase gene, bla(des-1), was cloned from whole-cell dna of isolate d3 and expressed in escherichia coli. purified beta-lactamase des-1, wi ... | 2002 | 12234847 |
| relationship of changes in interleukin-8 levels and granulocyte elastase activity in gingival crevicular fluid to subgingival periodontopathogens following non-surgical periodontal therapy in subjects with chronic periodontitis. | to determine the effect of scaling and root planing (srp) on the interrelations of subgingival periodontopathogens and both interleukin-8 (il-8) and granulocyte elastase activity in gingival crevicular fluid (gcf), and to assess their relations to the short-term treatment response in management of chronic periodontitis. | 2002 | 12354085 |
| comparison of microbial cultivation and a commercial pcr based method for detection of periodontopathogenic species in subgingival plaque samples. | microbiological laboratory procedures are involved in diagnosis and therapy control of progressive and refractory forms of periodontitis. in recent years techniques have been developed based on the detection of nucleic acids. the purpose of this study was to validate the commercially available micro-dent(r) test which employs probes for a. actinomycetemcomitans, p. gingivalis, p. intermedia, b. forsythus and t. denticola. | 2002 | 12354089 |
| initial effect of controlled release chlorhexidine on subgingival microorganisms. | little or no data exist on the ability of subgingival application of periochip (2.5 mg chlorhexidine gluconate in a biodegradable chip; astra pharmaceuticals, westborough, ma, usa) to suppress periodontopathic microorganisms. the present study compared the subgingival microbiota of periodontitis sites receiving the chlorhexidine chip plus scaling and root planing (sc/rp) or sc/rp alone. | 2002 | 12366861 |
| transmission of periodontal disease-associated bacteria from teeth to osseointegrated implant regions. | the presence of periodontopathic bacteria is a risk factor for peri-implantitis. the present study examined colonization by periodontopathic bacteria and their transmission from periodontal pockets to osseointegrated implant sulcus. | 2002 | 12381070 |
| oro-dental bacteria in various atherosclerotic arteries. | chlamydia pneumoniae dna has been detected in at least 40% of all major arteries affected by atherosclerosis, but several other microorganisms have also been detected. in this study, diseased vessels were evaluated for the presence of the dna of seven oro-dental bacteria and two nonoral bacteria. a polymerase chain reaction technique was employed using primer pairs based on 16s rrna genes. of 32 specimens tested, 10 (31.2%) were dna positive: seven for actinobacillus actinomycetemcomitans and th ... | 2002 | 12415477 |
| extract and identify ingredient from ligustrum lucidum ait and study its effect to periodontal pathogen. | to extract the effective ingredient (crystal i) from effective section (saponin) of ligustrum lucidum ait, identify the chemical structure of crystal i, study the effect of crystal i on p. gingivalis, b. forsythus and p. intermedia. | 2002 | 12425858 |
| presence and antibiotic resistance of porphyromonas gingivalis, prevotella intermedia, and prevotella nigrescens in children. | only limited information exists about the prevalence in children of pathogens associated with periodontitis. the aim of the present study was to determine by culture whether 8-11-year-old children carry porphyromonas gingivalis, prevotella intermedia, and/or p. nigrescens in samples from the gingiva and/or the buccal mucosa taken before, and after caries treatment and oral hygiene instruction. a second aim was to assess the proportion of subjects who had gram-negative anaerobes carrying the tet( ... | 2002 | 12445225 |
| effects of lipopolysaccharide extracted from prevotella intermedia on bone formation and on the release of osteolytic mediators by fetal mouse osteoblasts in vitro. | prevotella intermedia, a gram-negative obligate anaerobic black-pigmented oral bacterium, belongs to a small group of microorganisms that is closely associated with the initiation of periodontal diseases. lipopolysaccharide (lps), an outer membrane component, is one of the main virulence factors of this bacterium. the aim of this study was to examine the effects of prev. intermedia lipopolysaccharide, extracted by the hot-phenol-water method, on differentiation (alkaline phosphatase activity) an ... | 2002 | 12450517 |
| microbial susceptibility to calcium hydroxide pastes and their vehicles. | the aim of this study was to investigate the susceptibility of some microorganisms commonly isolated from root canals to calcium hydroxide in combination with several vehicles by the agar diffusion method. stainless-steel cylinders were placed on each inoculated agar medium. the test medications and their controls were placed inside the cylinders. the zones of growth inhibition were measured and recorded after the incubation period for each plate, and the results were analyzed statistically. ent ... | 2002 | 12470019 |
| porphyromonas gingivalis, bacteroides forsythus and other putative periodontal pathogens in subjects with and without periodontal destruction. | bacteria play an essential role in the pathogenesis of destructive periodontal disease. it has been suggested that not all bacteria associated with periodontitis may be normal inhabitants of a periodontally healthy dentition. in particular, porphyromonas gingivalis and actinobacillus actinomycetemcomitans have been isolated infrequently from subjects without periodontitis. the aim of the present study was to compare prevalence and proportions of a number of periodontal bacteria in periodontitis ... | 2002 | 12472995 |
| in vitro effect of laser irradiation on cementum-bound endotoxin isolated from periodontally diseased roots. | in a previous study, we evaluated the in vivo effects of an nd:yag laser on periodontal disease by measuring crevicular interleukin (il)-1beta levels before and after laser application. it was found that laser therapy was less effective than traditional scaling and root planing. these results might be due to incomplete removal of microbial residues and cementum-bound endotoxin on root surfaces by the laser. in this study, we explored the in vitro effectiveness of an nd:yag laser for the eliminat ... | 2002 | 12479628 |
| correlations between bacterial levels in autologous subgingival plaque and saliva of adult sudanese. | the aim of this study was to assess levels of oral bacteria and their correlations in paired samples of saliva and subgingival plaque in a population of adult sudanese. whole saliva and pooled subgingival plaque samples from six probing sites of one tooth in each jaw were obtained from 56 sudanese adults (mean age 35.2+/-8.9 years). levels of 24 oral bacteria in the autologous saliva and pooled plaque sample of each subject were assessed by dna probes and checkerboard dna-dna hybridization. ther ... | 2002 | 12483235 |
| similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study. | this study evaluated the similarity between the oral microbiota of young children and that of their adult caregivers. oral samples from children (174 dentate and 18 pre-dentate) aged 6-36 months and their caregivers in saipan were assayed using a dna probe assay. many species including streptococcus mutans, streptococcus sobrinus, actinomyces species, campylobacter rectus, fusobacterium nucleatum, prevotella intermedia, and porphyromonas gingivalis were detected in dentate and pre-dentate childr ... | 2002 | 12485330 |
| microbiota around root-form endosseous implants: a review of the literature. | although high success rates for root-form endosseous implants have been reported, failures occasionally occur, and these implants must be removed. at least 10% of the failures have been suggested to be the result of peri-implantitis. there is some evidence that periodontal pathogens, mainly those belonging to the group of gram-negative anaerobic rods, play a role in the etiology of peri-implantitis. this article provides an overview of the literature associated with common peri-implant microbiol ... | 2002 | 12507243 |
| antimicrobial activity of magainin analogues against anaerobic oral pathogens. | magainins are a family of potent antimicrobial cationic peptides that possess antimicrobial activity against a wide range of target organisms. in this study, the antimicrobial activity of synthetic magainin-mimetic compounds msi-751 and msi-774 was investigated against the periodontal pathogens porphyromonas gingivalis, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, eikenella corrodens, prevotella loescheii and prevotella intermedia. p. gingivalis was more susceptible to msi-751 ... | 2003 | 12507842 |
| [distribution of prevotella intermedia in the periodontal plaques of children and their parents]. | to investigate the distribution of prevotella intermedia (pi) among family members by culturing technique and biochemical identification. | 2000 | 12539530 |
| [study of bacteriology on local application of tinidazole stilus in treatment for periodontitis]. | the study was designed to evaluate the antiseptic efficacy of tinidazole stilus against periodontal pathogen. | 2001 | 12539636 |
| interleukin-10 receptor expression in human dental pulp cells in response to lipopolysaccharide from prevotella intermedia. | interleukin-10 receptor (il-10r) expression in human, dental pulp, fibroblast cultures was investigated by using the reverse transcriptase-polymerase chain reaction (rt-pcr) technique. after exposure to lipopolysaccharide (lps) from prevotella intermedia, the il-10r mrna levels increased after 4 h, peaked at 7 h, and dropped back to the unstimulated level at 24 h. maximal production of the il-10r protein in dental pulp fibroblast cultures was detected by western blot analysis after 12 h of lps s ... | 2003 | 12540220 |
| antimicrobial activity of ca(oh)2 dental cements: an in vitro study. | the effect of five commercial calcium hydroxide root canal medications (xr-spad, calcicur, hy-cal, root-cal, hypo-cal) and two calcium hydroxide mixtures (with water or glycerin) on the growth of (a) three anaerobic bacteria associated with endodontic infections; and (b) saliva bacterial strains was determined using the agar diffusion inhibitory test. statistically significant zones of bacterial growth inhibition for all the bacteria tested were observed. calcium hydroxide mixed with glycerin sh ... | 2003 | 12540221 |
| igg subclass specific antibody response to periodontopathic organisms in hiv-positive patients. | we previously reported an increased rate of progression of periodontal disease over an 18-month period in human immunodeficiency virus (hiv)-positive subjects compared to controls. the mechanism for disease progression and rapid tissue loss was unknown. data on the microbiological studies failed to show any significant difference in the microbial characteristics of the periodontal lesions in hiv-positive patients compared to hiv-negative controls. immunological analysis had identified neutrophil ... | 2002 | 12546094 |
| isotypic antibody response to plaque anaerobes in periodontal disease. | it has been suggested that locally produced immunoglobulin (ig)a could be more protective than igg and that there could be a relationship between crevicular fluid-specific iga levels and the onset of periodontal disease. this study was designed to investigate this hypothesis regarding specific immune responses towards 4 plaque anaerobes in gingival crevicular fluid and saliva from patients with periodontopathies and controls. | 2002 | 12546101 |
| hemolytic activity of prevotella intermedia and prevotella nigrescens strains: influence of abiotic factors in solid and liquid assays. | the influence of growth medium, hemin and menadione, blood source and atmosphere of incubation on the expression of hemolytic activity of 25 strains of prevotella intermedia and prevotella nigrescens was evaluated. the best hemolytic activity was observed for samples of both species growing in brain heart infusion agar and incubated in brewer-like anaerobic jars for 48 h. hemolysis was less intense and occurred later in the presence of hemin and menadione in solid media. beta-hemolysis was detec ... | 2003 | 12576156 |
| periodontal status in an italian young adult population. prevalence and relationship with periodontopathic bacteria. | to determine the prevalence of periodontitis in an italian young adult population and the relationship with actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in subgingival plaque. a full-mouth periodontal and oral examination was performed in 70 subjects. dental and behaviour habits were assessed with a standardised questionnaire. subgingival plaque samples were collected from the deepest pocket of the first molars in each quadrant with a sterile curette. ... | 2003 | 12578311 |
| isolation and characterization of hemolysin activated by reductant from prevotella intermedia. | the hemolysin from prevotella intermedia was partially purified from culture supernatant and then characterized. the hemolysin produced a clear beta-hemolytic zone on a blood agar plate. hemolytic activity was 2.5-fold greater in culture supernatant compared to that cell-associated. the isolation and purification procedure involved ammonium sulfate and polyethylene glycol precipitations and ion-exchange chromatographies on deae-sephacel and cm-sepharose. the activity of this hemolysin was stimul ... | 2003 | 12589956 |
| high prevalence of helicobacter pylori detected by pcr in the oral cavities of periodontitis patients. | helicobacter pylori has been associated with the development of peptic ulcers and gastric cancer. although it may be transmitted through the oral cavity, it is unknown whether the oral cavity acts as a permanent reservoir for this bacterium. the purpose of this study was to use nested polymerase chain reaction (pcr) to clarify whether the oral cavity acts as a reservoir for h. pylori. | 2003 | 12593608 |
| antibacterial effects of resinous retrograde root filling materials. | the effect of three resinous retrograde root filling materials on the growth of four obligate anaerobic bacteria associated with endodontic infections was determined by using the agar diffusion inhibitory test. samples of the following resinous materials (resin-modified, glass-ionomer cement, fuji ii lc; compomer, dyract; and composite resin, spectrum) were incubated for 1 week with the following anaerobic bacteria: fusobacterium nucleatum, porphyromonas gingivalis, p. endodontalis, and prevotel ... | 2003 | 12597711 |
| faropenem, a new oral penem: antibacterial activity against selected anaerobic and fastidious periodontal isolates. | the in vitro activity of faropenem, an oral penem, was compared with those of penicillin, co-amoxiclav, cefoxitin, clindamycin, erythromycin and metronidazole against 106 isolates of anaerobic pathogens involved in systemic infections. the organisms tested comprised porphyromonas gingivalis (29), prevotella spp. (eight), prevotella melaninogenica (seven), prevotella intermedia (five), actinomyces spp. (25), fusobacterium nucleatum (14), peptostreptococcus spp. (11), bacteroides ureolyticus (five ... | 2003 | 12615878 |
| kinetic and site-directed mutagenesis studies of prevotella intermedia acid phosphatase. | site-directed mutagenesis was used to examine the roles of the conserved histidine, arginine and cysteine residues in acid phosphatase from prevotella intermedia (piacp). the replacement of histidine and arginine residues resulted in the elimination of the piacp activity while the cysteine mutants retained activity. these results suggest that the histidine and arginine residues are essential for catalysis. | 2003 | 12625826 |
| bactericidal effects of different laser wavelengths on periodontopathic germs in photodynamic therapy. | this study was an attempt to clarify whether the bactericidal effects of photodynamic therapy (pdt) are wavelength or dose-dependent. we also attempted to create an optimised protocol for a light-based bactericidal modality to eliminate periodontal pathogens. cultures of actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphromonas gingivalis, prevotella intermedia, and streptococcus sanguis, were exposed to a he-ne laser (632.8 nm) with a 30 mw power output, a 100 mw diode laser a ... | 2003 | 12627274 |
| beta-lactamase production in prevotella and in vitro susceptibilities to selected beta-lactam antibiotics [corrected]. | this study looked for beta-lactamase production in 100 prevotella isolates. mics were determined for amoxycillin, ticarcillin, amoxycillin+clavulanate, cephalothin, cefuroxime, cefixime, cefpodoxime and cefotaxime using the reference agar dilution method (standard m11 a4, nccls). beta-lactamase activity was detected in 58 of the 100 isolates, 24 of 46 black-pigmented provotella and 34 of 54 non-pigmented prevotella. all beta-lactamase-negative strains were susceptible to all beta-lactam antibiot ... | 2003 | 12636990 |
| helicobacter pylori and campylobacter rectus share a common antigen. | aim: the aim of this study was to investigate the presence of antigens with immunological cross-reactivity in periodontopathogenic bacteria and helicobacter pylori, the pathogen associated with gastritis and peptic ulcers in human. materials and methods/results: among the putative periodontopathogens tested (actinobacillus actinomycetemcomitans, campylobacter rectus, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia and treponema denticola), cross-reactive bands were only ... | 2003 | 12654095 |
| acid-neutralizing activity during amino acid fermentation by porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum. | acid-neutralizing activity during amino acid fermentation by washed cells of porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum was studied. when the washed cells of these strains were anaerobically incubated in the presence of aspartylaspartic acid or glutamylglutamic acid for p. gingivalis, aspartic acid for p. intermedia and glutamic acid for f. nucleatum at an initial ph of 5.0 or 5.5, the ph of the incubation mixtures rose toward neutral. f. nucleatum had the highes ... | 2003 | 12654101 |
| microorganisms from canals of root-filled teeth with periapical lesions. | the objective of the present study was to identify the microbial flora within root canals of teeth with failed root-canal treatment and to determine the association of the various species with clinical features. | 2003 | 12656508 |
| proteolytic and hydrolytic enzymes from putative periodontal pathogens: characterization, molecular genetics, effects on host defenses and tissues and detection in gingival crevice fluid. | | 2003 | 12656998 |
| comparative clinical and microbiological effects of subgingival metronidazole application in adult periodontitis; 12-months results. | the aim of the present study was to evaluate the clinical and microbiological effects of subgingival application of 25% metronidazole dental gel as an adjunct to scaling and root planing (srp) in the treatment of adult periodontitis. eighty teeth in 18 patients were evaluated using a split mouth design. the test teeth received srp and a 25% metronidazole gel applied subgingivally on days 0 and 7. the control teeth received srp only. clinical and microbiological examinations were carried out befo ... | 2001 | 12666946 |
| subgingival microflora and antibody responses against periodontal bacteria of young japanese patients with type 1 diabetes mellitus. | periodontal disease is a complication of patients with type 1 diabetes mellitus (t1dm), although the mechanisms responsible for this relationship remain unclear. the aim of this study was to examine oral manifestations and the prevalence of periodontal pathogens from subgingival plaque samples and serum igg antibody levels against them in young japanese type 1 diabetic subjects. one hundred and seventeen japanese t1dm subjects (53 male, 64 female, mean age +/- sd, 16 +/- 6.5 years) participated ... | 2001 | 12666949 |
| serum phenytoin concentration and igg antibody titre to periodontal bacteria in patients with phenytoin-induced gingival overgrowth. | epileptic patients taking phenytoin with gingival-overgrowth and those without gingival-overgrowth were compared for daily drug dose, plasma total phenytoin concentration, plasma free-phenytoin concentration and serum igg antibody titre against 13 periodontal bacteria. significantly higher daily drug dose was noted in patients with gingival overgrowth (p < 0.05) when compared with those without overgrowth. in addition, both total and free forms of plasma phenytoin concentration were significantl ... | 2001 | 12666977 |
| induction of cyclooxygenase-2 mrna and protein expression in human pulp cells stimulated with black-pigmented bacteroides. | cyclooxygenase-2 (cox-2) is induced after the activation of cells by a variety of proinflammatory agents. recently, evidence has shown that cox-2 may play a role in the pathogenesis of pulpal inflammation. however, little is known regarding the mechanism of pulpal inflammation at the site of bacterial infection. the purpose of this study was to determine the effects of the supernatants from black-pigmented bacteroides (porphyromonas endodontalis, porphyromonas gingivalis, and prevotella intermed ... | 2003 | 12701770 |
| ovine periodontitis as a potential model for periodontal studies. cross-sectional analysis of clinical, microbiological, and serum immunological parameters. | : to investigate infection and host immunity patterns in sheep with naturally occurring "broken-mouth" periodontitis. | 2003 | 12702113 |
| the effect of a triclosan-containing dentifrice on the progression of periodontal disease in an adult population. | the aim of the present study was to determine the effect of unsupervised, long-term use of a 0.3% triclosan/2% copolymer dentifrice on the progression of periodontal disease in a general adult population. | 2003 | 12716333 |
| herpesviral-bacterial interactions in aggressive periodontitis. | human cytomegalovirus, epstein-barr virus and herpesvirus co-infections occur with significantly higher frequency in actively progressing than in stable periodontitis sites of adolescents and young adults. also, periodontal presence of cytomegalovirus and epstein-barr virus is associated with increased occurrence of subgingival porphyromonas gingivalis, bacteroides forsythus, dialister pneumosintes, prevotella intermedia, prevotella nigrescens, treponema denticola and actinobacillus actinomycete ... | 2003 | 12716334 |
| the effects of a new mouthrinse containing chlorhexidine, cetylpyridinium chloride and zinc lactate on the microflora of oral halitosis patients: a dual-centre, double-blind placebo-controlled study. | this study evaluated the microbial effects of a newly formulated mouthwash (halita) on oral halitosis patients. | 2003 | 12716335 |
| the detection of porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans in the supragingival plaque of children with and without caries. | the present study was undertaken to determine the presence of 3 periodontopathic bacteria in the supragingival plaques of 3- to 16-year-old children with different oral health conditions. | 2003 | 12723840 |
| fluorescence in situ hybridization (fish) for direct visualization of bacteria in periapical lesions of asymptomatic root-filled teeth. | whether micro-organisms can live in periapical endodontic lesions of asymptomatic teeth is under debate. the aim of the present study was to visualize and identify micro-organisms within periapical lesions directly, using fluorescence in situ hybridization (fish) in combination with epifluorescence and confocal laser scanning microscopy (clsm). thirty-nine periapical lesions were surgically removed, fixed, embedded in cold polymerizing resin and sectioned. the probe eub 338, specific for the dom ... | 2003 | 12724371 |
| adhesins encoded by the gingipain genes of porphyromonas gingivalis are responsible for co-aggregation with prevotella intermedia. | co-aggregation among bacterial cells caused by the adherence of one bacterial species to another is a potential colonization mechanism. several putative aggregation factors for co-aggregation between porphyromonas (por.) gingivalis and prevotella (pre.) intermedia were partially purified from por. gingivalis vesicles by gel filtration and affinity chromatography. antisera against the aggregation factors were made. analysis using these antisera revealed that 18 and 44 kda proteins might be respon ... | 2003 | 12724387 |
| cleavage of cd14 and lbp by a protease from prevotella intermedia. | periodontitis is an inflammatory disease caused by subgingival microorganisms and their components, such as lipopolysaccharide (lps). responses of the host to lps are mediated by cd14 and lps-binding protein (lbp). in this study, it was determined that proteases from a periodontal pathogen, prevotella intermedia, cleave cd14 and lbp, and thereby modulate the virulence of lps. culture supernatants from two strains of p. intermedia (atcc 25611 and 25261) cleaved cd14 and lbp in a concentration-dep ... | 2003 | 12728301 |
| effect of black-pigmented bacteria on the plasminogen-plasmin system in human pulp and osteoblastic cells. | to date, there have been relatively few studies addressing the presence and expression of the plasminogen-plasmin system at the site of bacterial infection. the aim of the present study was to investigate the effect of black-pigmented bacteria on the expression of the plasminogen-plasmin system in human pulp and osteoblastic cells. | 2003 | 12738955 |
| induction of interleukin-6 gene expression by pro-inflammatory cytokines and black-pigmented bacteroides in human pulp cell cultures. | to investigate the effect of pro-inflammatory cytokines and black-pigmented bacteroides on the expression of il-6 gene in human pulp fibroblasts. | 2003 | 12752649 |
| accidentally induced periodontitis in primary dentition: longitudinal examinations of periodontal bacteria and clinical conditions. | periodontitis is very rare in the primary dentition although it can be accidentally induced in young children. the purpose of this study was to describe a case of periodontitis in a 4-year-old child, which was accidentally induced by insertion of small plastic tubes into the dental cervix of the primary incisors during play, and to discuss the clinical, radiographic, and microbiological findings. removal of the plastic tubes resulted in resolution of the periodontal condition. prevotella nigresc ... | 2003 | 12752919 |
| generation of gingival t cell lines/clones specific with porphyromonas gingivalis pulsed dendritic cells from periodontitis patients. | it is well documented that in periodontitis lesions, most infiltrated gingival t cells are antigen-specific memory t cells. these cells play an important role as regulators and effector cells in the pathogenesis of periodontitis. in this study, we used dendritic cells (dcs) as antigen-presenting cells to generate human gingival t cell lines and clones specific for porphyromonas gingivalis from periodontitis patients. | 2003 | 12753363 |
| inhibitory effects of green tea catechins on protein tyrosine phosphatase in prevotella intermedia. | members of the prevotella intermedia group possess protein tyrosine phosphatase (ptpase). the purpose of this study was to investigate the effects of catechin derivatives from japanese green tea on the activity of ptpase in p. intermedia and related organisms. multilocus enzyme electrophoresis of alkaline phosphatase derived from p. intermedia, prevotella nigrescens, prevotella pallens and porphyromonas gingivalis revealed a species-specific migration pattern. among the tea catechin derivatives ... | 2003 | 12753472 |
| purification and partial characterization of a dipeptidyl peptidase from prevotella intermedia. | a peptidase hydrolyzed x-pro-p-nitroanilide was purified from the cell extract of prevotella intermedia atcc 25611 by ion-exchange chromatography and hydrophobic interaction chromatography. the purified enzyme exhibited a molecular size of 74 kda from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the maximum enzyme activity was found between ph 7.0 and ph 7.5. this peptidase was a serine enzyme and hydrolyzed lys-pro-p-nitroanilide, arg-pro-p-nitroanilide, and ala-pro-p-nitroanil ... | 2003 | 12753473 |
| chemical structure and immunobiological activity of lipid a from prevotella intermedia atcc 25611 lipopolysaccharide. | the novel chemical structure and immunobiological activities of prevotella intermedia atcc 25611 lipid a were investigated. a lipopolysaccharide (lps) preparation of p. intermedia was extracted using a phenol-chloroform-petroleum ether method, after which its purified lipid a was prepared by weak acid hydrolysis followed by chromatographic separations. the lipid a structure was determined by mass spectrometry and nuclear magnetic resonance to be a diglucosamine backbone with a phosphate at the 4 ... | 2003 | 12753913 |
| comparison of laboratory methods for detecting beta-lactamase-positive strains in the species prevotella intermedia sensu lato isolated from periodontal pockets. | | 2003 | 12783282 |
| evaluation of antibacterial activity of mangifera indica on anaerobic dental microglora based on in vivo studies. | the present study was carried out to compare the subgingival plaque microflora in mango leaf users. fifty subjects of both sexes, 25 of them used tooth brush and 25 used mango leaf as their home care hygiene device were included in the study. the microbiological evaluation for specific bacterial counts of actinobacillus actinomycetemcomitans, prevotella intermedia, porphyromonas gingivalis, fusobacterium nucleatum and peptostreptococcus micros were carried out for all subject. specific microbial ... | 2002 | 12785172 |
| can presence or absence of periodontal pathogens distinguish between subjects with chronic and aggressive periodontitis? a systematic review. | the purpose of this study was to determine to what extent the presence or absence of periodontal pathogens can distinguish between subjects with chronic and aggressive periodontitis. | 2002 | 12787203 |
| subgingival microbiota levels and their associations with periodontal status at the sampled sites in an adult sudanese population using miswak or toothbrush regularly. | little information is available on the effect of miswak use on gingival microbiota. we assessed levels of 28 oral bacteria in subgingival plaque of adult sudanese miswak (n = 38) and toothbrush users (n = 36) age range 20-53 years (mean 34.6 years) to study associations between these bacteria, oral hygiene method, and periodontal status at the sampled sites. a pooled subgingival plaque sample from 6 probing sites of 1 selected tooth in each jaw was obtained from each subject. whole genomic dna p ... | 2003 | 12790510 |
| acquisition and loss of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and prevotella intermedia over a 5-year period: effect of a triclosan/copolymer dentifrice. | the present study describes the natural history of porphyromonas gingivalis, actinobacillus actinomycetemcomitans and prevotella intermedia over a 5-year period and the effect of a triclosan/copolymer dentifrice on these organisms in a normal adult population. | 2003 | 12795792 |
| genomics of oral bacteria. | advances in bacterial genetics came with the discovery of the genetic code, followed by the development of recombinant dna technologies. now the field is undergoing a new revolution because of investigators' ability to sequence and assemble complete bacterial genomes. over 200 genome projects have been completed or are in progress, and the oral microbiology research community has benefited through projects for oral bacteria and their non-oral-pathogen relatives. this review describes features of ... | 2003 | 12799321 |
| microbiologic and radiographic analysis of ligature-induced peri-implantitis with different dental implant surfaces. | the goal of this study was to evaluate microbiota and radiographic peri-implant bone loss associated with ligature-induced peri-implantitis. | 2003 | 12814313 |
| lethal photosensitization in microbiological treatment of ligature-induced peri-implantitis: a preliminary study in dogs. | this pilot study evaluated, by culture testing, the effectiveness of lethal photosensitization for the microbiological treatment of peri-implantitis in dogs. experimental peri-implantitis was induced by ligature placement for 2 months. following ligature removal, plaque control was instituted by scrubbing with 0.12% chlorhexidine daily for 12 months. subsequently, mucoperiosteal flaps were elevated for scaling the implant surface. microbial samples were obtained with paper points before and afte ... | 2003 | 12816360 |
| lethal photosensitization of periodontal pathogens by a red-filtered xenon lamp in vitro. | the ability of helium-neon (he-ne) laser irradiation of a photosensitizer to induce localized phototoxic effects that kill periodontal pathogens is well documented and is termed photodynamic therapy (pdt). | 2003 | 12828662 |
| application of denaturing gradient gel electrophoresis (dgge) to the analysis of microbial communities of subgingival plaque. | denaturing gradient gel electrophoresis (dgge) was applied to the microbiologic examination of subgingival plaque. | 2003 | 12828664 |
| presence of the cfxa gene in bacteroides distasonis. | in this study we investigated the presence of the cfxa gene (encoding a class a beta-lactamase) in 73 strains of the bacteroides fragilis group belonging to the species b. distasonis (34), b. vulgatus (14), b. thetaiotaomicron (8), b. merdae (6), b. caccae (9) and b. ovatus (2) isolated from human intestinal microflora of healthy children and adults. employing specific primers to the cfxa gene, a 312-bp amplified fragment was obtained in 2 strains of b. vulgatus and 9 strains, the majority from ... | 2003 | 12837513 |
| the haem pigment of the oral anaerobes prevotella nigrescens and prevotella intermedia is composed of iron(iii) protoporphyrin ix in the monomeric form. | the haem pigment of porphyromonas gingivalis is composed of micro -oxo bishaem, [fe(iii)ppix](2)o, but the nature of that generated by prevotella species has not been established. mössbauer, raman and uv-visible spectrophotometry were used to characterize the haem pigment of prevotella intermedia and prevotella nigrescens. mössbauer and raman spectroscopy revealed the major haem species to be monomeric iron protoporphyrin ix, fe(iii)ppix.oh (haematin). the terminal growth ph of both species on b ... | 2003 | 12855722 |
| positive and negative bacterial associations involving dialister pneumosintes in primary endodontic infections. | dialister pneumosintes is an anaerobic gram-negative rod that has been recently implicated as a candidate endodontic pathogen. in this study, samples taken from abscessed teeth and infected root canals associated with asymptomatic or symptomatic periradicular lesions were examined for the occurrence of bacterial associations involving d. pneumosintes. dna was extracted from the samples, and the presence of d. pneumosintes and 16 other bacterial species was determined by means of species-specific ... | 2003 | 12877258 |
| accelerated alveolar bone loss in mice lacking interleukin-10. | interleukin-10 regulates pro-inflammatory cytokines, including those implicated in alveolar bone resorption. we hypothesized that lack of interleukin-10 leads to increased alveolar bone resorption. male interleukin-10(-/-) mice, on 129/svev and c57bl/6j background, were compared with age-, sex-, and strain-matched interleukin-10(+/+) controls for alveolar bone loss. immunoblotting was used for analysis of serum reactivity against bacteria associated with colitis and periodontitis. interleukin-10 ... | 2003 | 12885849 |
| antibiotic resistance of pathogenic bacteria from odontogenic infections in taiwan. | the purpose of this study was to evaluate the susceptibility of facultative and anaerobic odontogenic infectious flora to various antibiotics. we assessed 178 bacterial strains isolated from 74 patients with odontogenic infections. the e-test was used to determine susceptibility. the microbial flora was predominantly facultative gram-positive organisms and anaerobic gram-negative bacilli. the results of antimicrobial susceptibility test showed that ampicillin resistance was found with a very hig ... | 2003 | 12886961 |
| microbiological analysis of infected root canals from symptomatic and asymptomatic teeth with periapical periodontitis and the antimicrobial susceptibility of some isolated anaerobic bacteria. | the purpose of the present study was to investigate the correlation between the composition of the bacterial flora isolated from infected root canals of teeth with apical periodontitis with the presence of clinical signs and symptoms, and to test the antibiotic susceptibility of five anaerobic bacteria mostly commonly found in the root canals of symptomatic teeth against various substances using the e-test. microbial samples were taken from 48 root canals, 29 symptomatic and 19 asymptomatic, usi ... | 2003 | 12930519 |
| sensitivity of genera porphyromonas and prevotella to the bactericidal action of c-terminal domain of human cap18 and its analogues. | this paper reports the effect of the synthesized 27-amino acid sequence in the c-terminal domain of human cap18 (hcap18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera porophyromonas and prevotella. the domain binds lipopolysaccharides (lps) from porophyromonas gingivalis and porophyromonas circumdentaria as well as enterobacterial lps. two analogues of hcap18, designated ll/cap18 and ff/cap18, were also tested to determine whether additional ... | 2003 | 12930528 |
| coaggregation of streptococcus salivarius with periodontopathogens: evidence for involvement of fimbriae in the interaction with prevotella intermedia. | streptococcus salivarius is divided into two serological subgroups that carry either fibrils or fimbriae. although fimbriae have been observed on up to 50% of s. salivarius strains in the human oral cavity, no function has yet been assigned to them. to determine whether s. salivarius fimbriae have a role in adhesion, we examined the ability of s. salivarius to coaggregate with selected microorganisms involved in periodontal diseases. our results show that s. salivarius coaggregated with fusobact ... | 2003 | 12930529 |
| coronal microleakage assessed by polymicrobial markers. | studies have shown significant bacterial leakage following exposure of sealed root canals to artificial and natural saliva. the objective of this study was to determine contamination via bacterial migration in artificial saliva in endodontically treated teeth using different obturation techniques and sealers. a total of 144 extracted, human mandibular anterior teeth were cleaned and shaped to a #40 master apical file using hand and rotary instrumentation. one hundred and twenty teeth were divide ... | 2003 | 12937592 |
| prevotella intermedia meningitis associated with cerebrospinal fluid leakage in an adolescent. | | 2003 | 12938679 |
| the subgingival microflora in phenytoin-induced gingival hyperplasia. | microbial flora and gingival conditions were compared between a group of patients with phenytoin-induced gingival hyperplasia as a test group, a control group of patients who were administered phenytoin without gingival hyperplasia and a blank group who took no phenytoin and no gingival hyperplasia in mentally retarded patients. | 2003 | 12941071 |
| potential role of vascular endothelial growth factor, interleukin-8 and monocyte chemoattractant protein-1 in periodontal diseases. | host-mediated immunoinflammatory pathways activated by bacteria lead to destruction of the periodontal connective tissues and alveolar bone. the objective of this study was to elucidate the activation of the inflammatory processes in periodontal disease by quantitative assessment of cytokines and periodontopathogens. gingival crevicular fluids (gcf) and subgingival plaque samples were collected from patients with chronic periodontitis and gingivitis and from periodontally healthy sites. vascular ... | 2003 | 12962428 |
| immune response to fusobacterium nucleatum and prevotella intermedia in the sputum of patients with acute exacerbation of chronic bronchitis. | to assess the role of anaerobic bacteria in acute exacerbation of chronic bronchitis (aecb). | 2003 | 12970005 |
| quantitative real-time pcr using taqman and sybr green for actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tetq gene and total bacteria. | accurate quantification of bacterial species in dental plaque is needed for microbiological diagnosis of periodontal diseases. the present study was designed to assess the sensitivity, specificity and quantitativity of the real-time pcr using the geneamp sequence detection system with two fluorescence chemistries. taqman probe with reporter and quencher dye, and sybr green dye were used for sources of the fluorescence. primers and probes were designed for actinobacillus actinomycetemcomitans, po ... | 2003 | 14557000 |
| one case of gram-negative anaerobic spondylodiscitis with prevotella intermedia. | we report the case of a 45-year-old woman with spondylodiscitis at l1/l2, communicating with paravertebral, intravertebral and bilateral psoas abscesses. percutaneous computed tomography (ct)-guided abscess drainage and an intravenous antibiotic therapy with imipenem were performed. after removing the drainage at 2 weeks, the patient was discharged at 4 weeks with normalized blood parameters, normal temperature, and without need for analgesics. the underlying bacterium in the case was a very rar ... | 2003 | 14574605 |
| distribution of periodontal pathogens in korean aggressive periodontitis. | microbial associations in aggressive periodontitis versus different ethnic origins are substantially unknown. we undertook this study to determine the prevalence of seven putative periodontopathogens in korean patients and to evaluate microbial differences in localized and generalized aggressive periodontitis patients. | 2003 | 14584866 |
| flavobacterium johnsoniae gldh is a lipoprotein that is required for gliding motility and chitin utilization. | cells of flavobacterium johnsoniae move rapidly over surfaces by gliding motility. the mechanism of this form of motility is not known. six genes (glda, gldb, gldd, gldf, gldg, and ftsx) that are required for gliding have been described. tn4351 mutagenesis was used to identify another gene, gldh, which is required for cell movement. gldh mutants formed nonspreading colonies, and individual cells lacked the cell movements and ability to propel latex spheres along their surfaces that are character ... | 2003 | 14594839 |
| immunoglobulin g and a antibody responses to bacteroides forsythus and prevotella intermedia in sera and synovial fluids of arthritis patients. | the objective of the present study was to investigate immunoglobulin g (igg) and iga antibody immune responses to porphyromonas gingivalis, prevotella intermedia, bacteroides forsythus, and candida albicans in the sera of patients with rheumatoid arthritis (ra), the synovial fluid (sf) of patients with ra (ra-sf samples), and the sf of patients without ra (non-ra-sf samples). an enzyme-linked immunosorbent assay was used to determine igg and iga antibody levels in 116 serum samples from patients ... | 2003 | 14607865 |
| induction of interleukin-8 gene expression by black-pigmented bacteroides in human pulp fibroblasts and osteoblasts. | to investigate the effect of black-pigmented bacteroides on the expression of interleukin (il)-8 gene in human pulp fibroblasts and osteoblasts. | 2003 | 14641442 |
| antagonistic interactions among fusobacterium nucleatum and prevotella intermedia with oral lactobacilli. | microbiota residing in oral ecosystems show great complexity because of the mutual interdependence of certain microorganisms that might influence their presence or absence at a given site. the aim of this work was to evaluate possible antagonistic interactions among fusobacterium nucleatum and prevotella intermedia, isolated from periodontal pockets, and lactobacillus casei, l. salivarius, l. rhamnosus and l. plantarum isolated from saliva. different tests were carried out using each strain as a ... | 2003 | 14643404 |
| effects of eight antibacterial agents on cell survival and expression of epithelial-cell- or cell-adhesion-related genes in human gingival epithelial cells. | our previous studies suggest that little adverse effect on the growth of the periodontal ligament would be expected, if tetracycline, minocycline, ofloxacin, roxithromycin, clarithromycin, and azithromycin were topically administered to the periodontal pocket at their mic90 doses required to inhibit the growth of 90% of periodontopathic bacteria, including porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans. in the present study, we investigated the cytocida ... | 2004 | 14687228 |
| in vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. | the aim of this study was to investigate in vitro the antimicrobial activity of 0.2%, 1%, and 2% chlorhexidine gluconate (chx gel and chx liquid), against endodontic pathogens and compare the results with the ones achieved by 0.5%, 1%, 2.5%, 4%, and 5.25% sodium hypochlorite (naocl). a broth dilution test was performed, and the timing for irrigants to kill microbial cells was recorded and statistically analyzed. both 2.0% gel and liquid formulations eliminated staphylococcus aureus and candida a ... | 2004 | 14716261 |
| in vitro activation of the hemolysin in prevotella nigrescens atcc 33563 and prevotella intermedia atcc 25611. | hemolytic activity was evaluated in the putative periodontopathogens prevotella intermedia and prevotella nigrescens. whole cells of both species present weak hemolytic activity evidenced only by solid media assays after 48 h of bacterial growth or after 5 h of interaction with erythrocytes at 37 degrees c in liquid assays. in this work we show that the use of crude extract allowed the detection of a higher hemolytic activity for p. intermedia, but surprisingly not for p. nigrescens. incubation ... | 2004 | 14759706 |