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rapid salt-based mini-scale gardnerella vaginalis dna isolation procedure. 19958588908
evaluation of a rapid diagnostic test for bacterial vaginosis.to evaluate a new rapid diagnostic test for bacterial vaginosis.19968605135
streptococcus agalactiae: a vaginal pathogen?the significance of streptococcus agalactiae as an aetiological agent in vaginitis was evaluated. a total of 6226 samples from women who presented with vaginal symptoms was examined. the presence of >10 leucocytes/high-power field (h.p.f.) was taken to be the criterion of active infection. s. agalactiae was isolated from 10.1% of these samples. the isolation rates of other common pathogens such as candida spp., gardnerella vaginalis and trichomonas spp. were 54.1%, 27.2% and 4.2%, respectively, ...19968636937
bacteremia shortly after placental separation during cesarean delivery.to assess frequency, risk factors, and microbiology of bacteremia within 15 minutes of placental separation during cesarean delivery.19968677085
pelvic inflammatory disease in adolescents. 19968681606
bacterial vaginosis in pregnancy: distribution of bacterial species in different gram-stain categories of the vaginal flora.vaginal swabs for microbiological culture were taken from 174 pregnant women whose vaginal flora had been evaluated by gram's stain; 50 had grade iii flora (bacterial vaginosis, bv), 50 grade ii (intermediate), 41 had vaginal flora graded as abnormal which then reverted to grade i (revertants) and 33 had normal flora (controls). the aim was to determine whether bacterial species isolated from women with grade ii flora differed from those with grade iii flora. isolation of lactobacillus spp. decr ...19968683547
[gardnerella vaginalis skenitis]. 19968695695
[gardnerella vaginalis: transport, microscopy, testing resistance].g. vaginalis is an important pathogen in the aetiology of bacterial vaginosis. therefore, we investigated the influence of transport systems in isolation, a scoring system for gram stains, and susceptibility to antimicrobial agents. the comparison between a simple (transwab) and a sophisticated (port-a-cul) system showed no differences with regard to for instance enterococcus faecalis or escherichia coli; however, isolation of g. vaginalis, a fastidious microorganism, was significantly higher (a ...19948719002
composition of the lactobacillus acidophilus complex isolated from vaginal flora.one hundred and fifty human vaginal samples containing a diversity of pathogens or nonpathogens (gardnerella vaginalis, streptococcus sp., staphylococcus sp., candida albicans. mycoplasma sp.) were examined for their content in lactobacilli of the lactobacillus acidophilus complex. although all samples contained lactobacilli, strains of the l. acidophilus complex were present in only twenty-nine cases. isolates were further characterized and compared with type strains or reference strains in an ...19968722308
papillomavirus in cervicovaginal smears of women infected with human immunodeficiency virus.it has been described that women infected with the human immunodeficiency virus (hiv) present more frequent cytological abnormalities in cervicovaginal smears, generally related to infection by human papillomavirus (hpv). the present work is a study of cervicovaginal smears of 147 hiv-seropositive women submitted to routine gynecological examinations. the smears were stained by the papanicolaou method. cytopathic effects of hpv were found in 38 (25.8%) cases. nuclear atypias of cervical intraepi ...19958731285
[balanitis and infectious agents. a prospective study of 100 cases].the aim of this study was: 1) to evaluate the rate of micro-organism isolation in 100 patients consulting for balanitis at the centre of sexually transmitted diseases at the st. louis hospital in paris in comparison with that of micro-organisms isolated in 60 men without balanitis; 2) to search for a possible correlation between the clinical aspect of the disease and the nature of the infectious agent identified.19958745679
genomic dna fingerprint analysis of biotype 1 gardnerella vaginalis from patients with and without bacterial vaginosis.of the 20 biotype 1 gardnerella vaginalis isolates analyzed, 10 from patients with bacterial vaginosis and 10 from patients without bacterial vaginosis, none shared the same dna fingerprint. however, a 1.18-kb hindiii fragment was common among 18 of the 20 biotype 1 isolates in a restriction fragment length polymorphism analysis with a 7.9-kb g. vaginalis dna probe.19968748302
bacteriological epidemiology and treatment of bacterial vaginosis.one hundred symptomatic women with clinical bacterial vaginosis (bv) were enrolled in this study. the bacteriological epidemiology of bv and efficacy of oral or vaginal administration of antimicrobial agents for treatment of bv were investigated. the epidemiology of bv was investigated before antimicrobial therapy. fifty patients were treated with oral administration of metronidazole (mtn), 500 mg twice a day, and 50 patients with 2% clindamycin (cldm) phosphate in a vaginal cream, 5 g once a da ...19968751270
[detection of bacterial vaginosis in gram stained vaginal smears and papanicolaou stained cervical smears].to determine the sensitivity and specificity of diagnosis of bacterial vaginosis (bv) by papanicolaou (pap)-stained cervical smears and gram-stained vaginal smears according to clinical criteria.19968758276
pathogenic potential of gardnerella vaginalis on the female urogenital system.the prevalence of certain characteristics of genital discharge like (a) watery variety in sexually transmitted disease (std) clinic 34% and in gynaecology clinic 26.6%, (b) fishy odour in std clinic 29.2% and in gynaecology clinic 12.2% and (c) ph > 4.5 in std clinic 53.6% and in gynaecology clinic 43% was notable. on the other hand, occurrence of "clue cells" (in std clinic 41.4% and in gynaecology clinic 39.5%) did not show difference in the aforesaid clinics. the preponderance of watery disch ...19968776033
polyphasic approach to the classification and identification of gardnerella vaginalis and unidentified gardnerella vaginalis-like coryneforms present in bacterial vaginosis.a taxonomic study of gardnerella vaginalis and g. vaginalis-like coryneforms was performed in order to clarify the phylogenetic affiliation of these organisms and to improve future identification. we examined 50 strains by performing whole-cell protein and fatty acid analyses, a 16s rrna sequence analysis, and an extensive phenotypic characterization analysis. the results of both chemotaxonomic techniques which we used divided the organisms into two main clusters, and the 16s rrna sequence analy ...19968782675
in-vitro activity of purpuromycin and mdl 63,604 against microorganisms that cause vaginitis and vaginosis.purpuromycin and its semi-synthetic derivative mdl 63,604 had in-vitro activity similar to that of amphotericin b against isolates of candida albicans. mdl 63,604 had activity similar to that of metronidazole against trichomonas vaginalis. both compounds were very active against most species of gram-positive and gram-negative anaerobes and against gardnerella vaginalis. mdl 63,604 had significantly lower mics than purpuromycin against t. vaginalis and most of the bacteria, probably due to antago ...19958821607
defining bacterial vaginosis: to bv or not to bv, that is the question.the history of bacterial vaginosis (bv), now extending over more than 40 years, has been remarkable not only in terms of repeatedly changing the name of the bacterium that we now know as gardnerella vaginalis but also in relation to what is thought to constitute the condition, what it should be called and how the diagnosis can best be made. the composite clinical criteria are often confusing for the nonspecialist, provide room for inter-observer error, and misinterpretation of just one criterion ...19968876352
bacterial vaginosis: prevalence in an italian population of asymptomatic pregnant women and diagnostic aspects.the present study has been carried out with the main objective of determining the prevalence rate of bacterial vaginosis (bv) in a population of asymptomatic pregnant women (8th to 9th month of pregnancy). out of a total 1,441 patients examined bv was found to be present in 70 (4.9%). the diagnosis was made when an analysis of the vaginal discharge revealed the simultaneous presence of at least three out of four of the following laboratory indices: (1) direct gram stain positive (bacterial flora ...19968891543
vaginal microflora associated with bacterial vaginosis in japanese and thai pregnant women.the vaginal flora of 118 japanese and 208 thai pregnant women were investigated for the presence of bacterial vaginosis (bv), bv-associated organisms, and bv-associated enzyme. a similar prevalence of bv was found among the japanese (13.6%) and thai women (15.9%). the microbial flora of women with bv were complex; the mean number of isolates recovered in the bv group was approximately 2 times more than that in a group of healthy women. prevotella species, porphyromonas species, peptostreptococcu ...19968909838
etiologic study of genitourinary infections in women of childbearing age in bobo-dioulasso, burkina faso, 1992.genitourinary infections have a major impact on public health, especially in africa. relative distribution of the different pathogens is unknown in bobo-dioulasso.19968919743
[preliminary study on low-trait genital infection and cervical epithelial dysplasia in women from the parakanä tribe of south america].we evaluated the occurrence of low-trait genital infection and cervical epithelial dysplasia in women from a south-american indian tribe in the brazilian amazonia.19968935669
comparison of in vitro activities of du-6859a and other fluoroquinolones against japanese isolates of anaerobic bacteria.the in vitro activity of du-6859a, a new fluoroquinolone, was compared with those of other fluoroquinolones against clinical isolates of anaerobic bacteria and gardnerella vaginalis. du-6859a was the most active agent; it inhibited 90% of isolates of almost all species tested, including bacteroides fragilis at < or = 0.39 micrograms/ml. although the other quinolones tested were active against gram-positive anaerobes, inhibiting their growth at < or = 1.56 micrograms/ml, these agents were less ac ...19968953104
[gardnerella vaginalis in reports of cervico-vaginal cytology].gardnerella vaginalis is a microorganism of great importance because of its participation in some gynecological diseases. data contained in 3421 cervicovaginal cytology reports obtained from the profamilia clinic, cartagena, colombia between june 1993 and february 1994 were analyzed retrospectively and analytically. cells suggestive of gardnerella vaginalis were found in 723 reports, the greatest proportion being in the 25-29, 30-34, 35-39 year-old age groups (without statistical significance). ...19968964347
growth inhibition of metronidazole-susceptible and metronidazole-resistant strains of gardnerella vaginalis by lactobacilli in vitro.metronidazole resistance was produced in susceptible gardnerella vaginalis after subculture in the presence of metronidazole. metronidazole-resistant gardnerellae were less susceptible to growth inhibition by lactobacillus culture filtrates. a low ph (+/- 4) and lactic acid accounted for 60 to 95% of inhibitory activity, and h2o2 accounted for only 0 to 30%. however, in the presence of myeloperoxidase, h2o2-producing lactobacilli decreased the viability of metronidazole-susceptible gardnerellae ...19968975601
bacterial vaginosis.bacterial vaginosis (bv) is an alteration of the vaginal flora, where the normally predominant lactobacilli are replaced by a cocktail of organisms including gardnerella vaginalis and anaerobes. it presents with a grey, homogenous, offensive vaginal discharge that has a raised ph. however, around half the women with this condition are asymptomatic. diagnosis is best made by microscopic examination of a gram-stained smear of vaginal secretions. treatment is with metronidazole or clindamycin. the ...19968983322
biotypes of gardnerella vaginalis isolated from urinary tract.a modified scheme is proposed for biotyping gardnerella vaginalis isolated from urinary tract of symptomatic and asymptomatic women based on detection of hippurate hydrolysis, beta-galactosidase (onpg) and lipase, and fermentation of arabinose, galactose and xylose. thirty biotypes were found among 73 strains. the distribution of biotypes was similar in both populations but the biotypes 1h, 5g and 7g were found more frequently in women without symptoms of urinary tract infection.19968986106
specific immune response against gardnerella vaginalis hemolysin in patients with bacterial vaginosis.our goal was to study the mucosal host response in bacterial vaginosis by evaluating the presence of a specific immune response elicited against the gardnerella vaginalis hemolysin in vaginal fluids of patients and by verifying its correlation with usual criteria adopted to diagnose bacterial vaginosis.19968987947
[screening for bacterial vaginosis in a population of pregnant women reporting to cardiotocographic tests].of 753 patients repeatedly controlled by nonstress test the incidence of bacterial vaginosis amounted to 11.28%. as regards the bacterial kinds isolated we have not noticed expressive differences in relation to the different periods of pregnancy, with the exception of the candida albicans that instead has been the more frequent and in constant increase. before the 30th week of pregnancy until the 35th, more variety of bacterial flora, with prevalence of aspecific vaginitis has been observed. the ...19969005375
[microbiological study of some microorganisms implicated in sexually transmitted diseases among the female prison population].background: sexually transmitted diseases (std) are of over greater interest, particularly since the appearance of the human immunodeficiency virus ( hiv) in the last decade. asymptomatic or oligosymptomatic infections are very frequent, overall in women. the aim of this study was to know the prevalence of certain std producing microorganisms in a female penitentiary population, the clinical characteristics and evaluate the risk factors which may be found in this population. methods: one hundred ...19969011204
balanitis and balanoposthitis: a review. 19969038655
[clinical and microbiological evaluation of bacterial vaginosis in women in a hospital from rio cuarto, argentina].one hundred and seventy nine women of discharge vaginal were studied. endocervical and exocervical samples were obtained. the vaginal fluids samples were processed by the following tests: ph, fishy odor, clue cells observation was done by gram reaction. gardnerella vaginalis was identified through biochemical tests. g. vaginalis (23.4%), c. albicans (21.8%), t. vaginalis (14.5%), n. gonorrhoeae (5.5%) and c. trachomatis (11.7%), were found in 42 women. a 7.1% of forms compatible with mobiluncus ...19969053005
semisynthetic derivatives of purpuromycin as potential topical agents for vaginal infections.purpuromycin (1) is an antibiotic with a broad spectrum of antimicrobial activity, encompassing bacteria, fungi, and protozoa, particularly those involved in vaginal infections. with the aim of enhancing the solubility and reducing the serum binding, a chemical program of modifications was undertaken on the natural compound, and a new interesting series of derivatives at the naphthoquinone system was synthesized and evaluated as potential topical agents for vaginal infections. in particular thre ...19979083486
reactive arthritis due to gardnerella vaginalis. a case-report. 19979085451
[gardnerella vaginalis in a newborn infant: contamination or infection, apropos of a case]. 19969092313
identification and quantification of bifidobacterium species isolated from food with genus-specific 16s rrna-targeted probes by colony hybridization and pcr.a bifidobacterium genus-specific target sequence in the v9 variable region of the 16s rrna has been elaborated and was used to develop a hybridization probe. the specificity of this probe, named lm3 (5'-cgggtgcti*cccactttcatg-3'), was used to identify all known type strains and distinguish them from other bacteria. all of the 30 type strains of bifidobacterium which are available at the german culture collection deutsche sammlung von mikroorganismen und zellkulturen, 6 commercially available pro ...19979097423
what is normal vaginal flora?to observe the composition of the vaginal flora of healthy women over time, and in relation to hormonal changes, sexual activity, and hygiene habits.19979155551
evidence for a commensal, symbiotic relationship between gardnerella vaginalis and prevotella bivia involving ammonia: potential significance for bacterial vaginosis.six strains of prevotella bivia and 4 of gardnerella vaginalis were examined for nutrient substrate utilization as part of ongoing studies on the pathogenesis of bacterial vaginosis. addition of single amino acids to vaginal defined medium (vdm) was stimulatory to the growth of p. bivia but not to g. vaginalis. however, peptides significantly promoted the growth of both organisms. growth of p. bivia in vdm and vdm supplemented with either amino acids or peptone was accompanied by net ammonia pro ...19979203662
vaginal ph as a marker for bacterial pathogens and menopausal status.our purpose was to confirm the elevation of vaginal ph expected in patients with bacterial pathogens in premenopausal women and to examine the relationship of serum follicle-stimulating hormone and estradiol levels to vaginal ph in menopausal patients without and with hormone replacement therapy.19979215184
genotypic differentiation of gardnerella vaginalis by amplified ribosomal dna restriction analysis (ardra).in total 34 strains of gardnerella vaginalis were analyzed with various molecular techniques in order to find the possibility of dividing this single species into different genotypes. classical ribotyping, pcr-ribotyping and restriction analysis of 16s-23s rrna intergenic spacer sequences were all unsuccessful in genotype differentiation of these bacteria. only amplified ribosomal dna restriction analysis (ardra) was suitable in recognizing different g. vaginalis genotypes. at least 3-4 genotype ...19979215588
garderella vaginalis bacteremia in an adult male. 19979228486
multiple c-terminal serine phosphorylation accompanies both protein kinase c-dependent and -independent activation of cytosolic 85 kda phospholipase a2 in macrophages.exposure of mouse macrophages to either phorbol ester or certain bacteria was previously shown to cause increased phosphorylation of the cytosolic 85 kda phospholipase a2 as well as a stable increase in its catalytic activity. we have now attempted to map the major phosphorylation sites on the enzyme in such cells. phosphorylation occurred on serine residues without a detectable increase in either phosphothreonine or phosphotyrosine. after cnbr cleavage five fragments showed increased 32p labell ...19979230120
[a comparison of 2 clinical laboratory methods in the diagnosis of bacterial vaginosis].to determine the sensitivity, the specificity, and positive and negative predictive values of two laboratory methods used to diagnose bacterial vaginosis; gram stain and the gardnerella vaginalis culture, in comparison with the clinical sings of vaginal discharge; homogeneous secretion, ph > 4.7, positive amine test and the presence of clue cells.19979254139
microbiology of semen specimens from males attending a fertility clinic.the relationship between semen quality, pyospermia and bacteriology was studied in 201 semen specimens from male patients attending a fertility clinic. semen quality parameters were within normal limits in 115 (57%) patients, slightly reduced in 60 (30%), and 26 (13%) had findings indicating reduced fertility. twelve patients (6%) had pyospermia. in 182 patients, 552 microorganisms were detected, including enterobacteriaceae (2.8%), gardnerella vaginalis (9.6%), chlamydia trachomatis (1.6%), myc ...19979269303
ultrastructural characteristics of gardnerella vaginalis infection in the heterosexual couple.adhesion and penetration of gardnerella vaginalis into the male urethral and female vaginal epithelial cells were evaluated in a prospective cohort study of infected females and their sexual partners. vaginal secretions of 10 women with culture proven g. vaginalis infection and semen samples of their asymptomatic husbands were analyzed with conventional optical and electron microscopy. g. vaginalis was isolated in 50% of the male sexual partners of women harboring the microorganism. g. vaginalis ...19979272231
[the microbial etiology of genital discharges in nouakchott, mauritania].the survey carried on 579 patients of both sexes, consulting for genital discharges (spontaneous or referred), showed that in 61.1% men cases, std agents were isolated (neisseria gonorrhoeae 51.5%) and 64.4% in women cases (gardnerella vaginalis 24%, candida albicans 20%. trichomonas vaginalis 14%). 17% of neisseria gonorrhoeae strains were penicillinase-producing (ppng).19979289258
vaginal microflora in postmenopausal women who have not received estrogen replacement therapy.we studied the vaginal microflora of 73 postmenopausal women who had never received estrogen replacement therapy. the median age of these women was 67 years, and none of them had bacterial vaginosis. lactobacilli were detected in 36 (49%) of these women at a mean concentration of 10(5.7) cfu/g of vaginal fluid. h2o2-producing lactobacilli were recovered from 38% of the women. some of the other organisms that were recovered, including gardnerella vaginalis (27% of the women), ureaplasma urealytic ...19979310650
metronidazole. a therapeutic review and update.the nitroimidazole antibiotic metronidazole has a limited spectrum of activity that encompasses various protozoans and most gram-negative and gram-positive anaerobic bacteria. metronidazole has activity against protozoans like entamoeba histolytica, giardia lamblia and trichomonas vaginalis, for which the drug was first approved as an effective treatment. anaerobic bacteria which are typically sensitive are primarily gram-negative anaerobes belonging to the bacteroides and fusobacterium spp. gra ...19979360057
molecular evolution of the tet(m) gene in gardnerella vaginalis.five clinical isolates of gardnerella vaginalis known to carry the tetracycline resistance determinant tet m were examined by hybridization and nucleotide sequencing. four of the strains carried tet(m) genes with identical sequences. the two versions of the tet(m) gene found in g. vaginalis did not show complete identity with other published tet(m) sequences, but showed mosaic structures with regions of homology to tet(m) gene sequences from tn916, tn1545 and the american type plasmid found in n ...19979372426
[vulvovaginitis].candidiasis, infection due to trichomonas vaginalis and bacterial vaginosis (gardnerella vaginalis and/or other species) represent the major three causes of vulvo-vaginitis. other are rare bacterial infections and non infectious vaginitis such as allergic and post-menopausal vaginitis with epithelial atrophy. clues for the diagnosis include the clinical features of vaginal discharge, cytological examinations, bacterial and fungal cultures. only t. vaginalis seems to be responsible of sexually tr ...19979406511
[laboratory diagnosis of bacterial vaginosis].microbiological analysis of discharge from the genitals in passenger car conductors (5787 women and 1496 men aged 20 to 40) showed gardnerella vaginalis to be the most frequent agent of urogenital infection. it was isolated in 26% cases, whereas trichomonas were isolated in 2.5%, fungi in 1.5%, and gonococci in 0.27% cases. the diagnosis of gardnerellosis is reliable if the key cells are found. monoinfection with g. vaginalis was diagnosed in 80% patients, the overwhelming majority (74%) of carr ...19979410459
[diagnosis of chlamydia and gardnerella infection in patients with acute gynecological diseases].chlamydial infection was diagnosed by indirect immunofluorescence in 26.3% and gardnerella infection in 28.7% of patients with acute gynecological diseases. the degree of vaginal contamination in patients with chlamydiasis was virtually the same as in all other examinees, whereas in patients with gardnerellosis the third and fourth degrees of purity predominated. hence, assessment of the vaginal contamination (purity) is an additional criterion for screening for gardnerellosis but providing no c ...19979410461
impact of metronidazole therapy on preterm birth in women with bacterial vaginosis flora (gardnerella vaginalis): a randomised, placebo controlled trial.to ascertain whether metronidazole treatment of women with a heavy growth of gardnerella vaginalis during mid-pregnancy would reduce the risk of spontaneous preterm birth.19979422018
therapy of bacterial vaginosis. 19989511031
[in vitro inhibition of the growth of gardnerella vaginalis by bacteriocins produced by strains of pseudomonas aeruginosa].pseudomonas aeruginosa with inhibitory capacity in vitro was studied on gardnerella vaginalis strains.19979527372
immunoglobulin a response against gardnerella vaginalis hemolysin and sialidase activity in bacterial vaginosis.the aim of this study was to investigate the correlation between the immunoglobulin a immune response to gardnerella vaginalis hemolysin and sialidase activity in vaginal fluids from patients with bacterial vaginosis.19989539518
few microorganisms associated with bacterial vaginosis may constitute the pathologic core: a population-based microbiologic study among 3596 pregnant women.to evaluate the association between various microorganisms isolated from the genital tract in pregnant women with bacterial vaginosis.19989539529
diabetes and pregnancy: prophylaxis of genital infections.diabetic pregnant women have many potential reasons to have genital infections such as poor metabolic control and impairement of leucocyte function. relative immune deficiency exists in pregnancy. this study was designed to test the hypotheses that pregnant patients with insulin-dependent diabetes have a higher rate of ante partum genital infections when compared with a pair-matched control population. two groups of pregnant women consisting of 23 patients with and 23 without diabetes mellitus, ...19979542260
[prevalence of bacterial vaginosis in an obstetric population of barcelona].to investigate the prevalence and risk factors of bacterial vaginosis (bv) in a pregnant population of barcelona (spain).19989547730
[bacterial vaginosis: where are we?]. 19989547733
the role of gardnerella vaginalis in nonspecific vaginitis in intra uterine contraceptive device users.two hundred forty patients who had intra uterine contraceptive device (iucd) and manifested of nonspecific vaginitis were investigated for the presence of g. vaginalis. pure growth of this organism was obtained in 14(5.8%) cases while 116(48.3%) cases showed this organism in association with other organisms e.g. esch. coli (11.7%), klebsiella (9.2%), candida (9.2%), strept. faecalis (7.3%), proteus species (5.8%) and staph. albus (5%).19989581079
clinical findings in female genital schistosomiasis in madagascar.to assess the morbidity of s. haematobium infection in women of reproductive age (15-49 years) in the western part of madagascar, the village of betalatala with a prevalence of urinary schistosomiasis in women of 75.6% (95% confidence limit 69.3 to 81.9%) was compared with a neighbouring village with similar socio-economic characteristics and a prevalence of 5.0% (95% confidence limit 0 to 11.75%). the women were questioned in malagasy about obstetrical history and urogynecological symptoms. the ...19989623935
use of a continuous-culture biofilm system to study the antimicrobial susceptibilities of gardnerella vaginalis and lactobacillus acidophilus.gardnerella vaginalis and lactobacillus acidophilus have been shown to grow to high titers in a simple biofilm system. this system was used in the present investigation to compare the biofilm-eradicating concentrations (becs) of amoxicillin, clindamycin, erythromycin, and metronidazole to standard tube mic and minimum bactericidal concentration (mbc) results. with the lactobacillus, the bec/tube mbc ratio was at least 16:1, while for g. vaginalis the ratio varied from 2:1 to 512:1. the simple co ...19989624489
[a new zoonosis--investigation of gardnerella vaginalis disease of fox. v. studies serotype of gardnerella vaginalis in fox].145 strains gardnerella vaginalis isolated in foxes were isolated from 13 main farms raising foxes in six provinces (regions), china, after antigenicity and immunogenicity of the strains were measured, 1-3 appropriate strains were selected from each farm raising foxes for serotype studies. cross agglutinin absorption test confirmed that selected 26 strains gardnerella vaginalis were divided into three serotypes and then the representing strains were used to produce typing serum. among remaining ...19969639824
chlamydia trachomatis: probable cause of prostatitis.seventy-eight men with symptoms of chronic or subacute prostatitis were enrolled. investigations for the presence of chlamydia trachomatis in urethral swabs were carried out. the expressed prostatic secretions were additionally examined for mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, other gram-negative and gram-positive bacteria, trichomonas vaginalis, yeast-like fungi and leucocyte count. furthermore, all patients were evaluated for the presence of serum anti-chlamydial ...19989671250
cytologic findings in a neovagina created with vecchietti's technique for treating vaginal aplasia.to report long-term cytologic findings after treatment of congenital vaginal aplasia or mayer-rokitansky-küster-hauser syndrome (mrkh) using progressive traction of the vulvar tissues, or vecchietti's technique (vt).19989684582
female genital tract bacterial coisolates with candida albicans in patients without clinical vaginitis.in vitro, candida albicans has demonstrated the ability to inhibit replication of selected bacteria. little information exists on the impact of c. albicans on the vaginal bacterial flora in vivo. the purpose of this study is to identify the coexisting bacterial flora when c. albicans is isolated from vaginal cultures submitted to a hospital-based testing facility for reasons other than vulvovaginitis.19989702585
preterm birth: associations with genital and possibly oral microflora.opportunistic pathogenic microbes are indigenous to the female lower genital tract and etiologic in many types of pelvic infections and, apparently, a portion of preterm birth (ptb) cases. bacterial vaginosis (bv) is a clinical syndrome based on an altered genital microflora in which gardnerella vaginalis; anaerobic species primarily among prevotella, porphyromonas, bacteroides, peptostreptococcus, and mobiluncus; mycoplasma hominis; and ureaplasma urealyticum become predominant in vaginal secre ...19989722706
characterization of actinomyces turicensis and actinomyces radingae strains from human clinical samples.whole-organism protein electrophoresis was used to compare and group unidentified coryneform bacteria resembling gardnerella vaginalis and various actinomyces and arcanobacterium species. the obtained clusters of strains were further characterized by whole-cell fatty acid analysis and a variety of biochemical tests. species-specific oligonucleotide probes based on 16s rrna gene sequences were designed. the results demonstrate that the majority of the isolates belonged to actinomyces turicensis; ...19989731291
bacterial vaginosis: association with adverse pregnancy outcome.bacterial vaginosis is the most common lower genital tract infection encountered among women of reproductive age. this condition can best be considered as a vaginal syndrome associated with an alteration of the normal vaginal flora rather than an infection specific to any one microorganism. bacterial vaginosis is a clinical condition with a complex microbiology that is characterized by a reduced concentration of a normally abundant lactobacillus species along with high concentrations of gram-neg ...19989738988
acquisition of iron by gardnerella vaginalis.six gardnerella vaginalis strains were examined for the ability to utilize various iron-containing compounds as iron sources. in a plate bioassay, all six strains acquired iron from ferrous chloride, ferric chloride, ferrous sulfate, ferric ammonium citrate, ferrous ammonium sulfate, bovine and equine hemin, bovine catalase, and equine, bovine, rabbit, and human hemoglobin. all six strains also acquired iron from human lactoferrin, but not from human transferrin, as determined by a liquid broth ...19989746616
reiter's syndrome induced by gardnerella vaginalis. 19989751477
[value of a computer-assisted screening method (papnet) for the detection of infectious cervico-uterine smears].there have been several reports regarding the accuracy of the papnet system applied to the screening for cancerous and precancerous lesions. based on neuronal networks, this computerized tool was initially trained to select atypical cells. it has been approved in the usa for the re-screening of cervical smears for quality assurance. however, its particular behaviour has not been frequently studied when the system is designed to detect frequent infectious organisms. we report the results of re-sc ...19989754374
bacterial pathogens in male patients with urethritis in istanbul. 19989773441
[the role of lower genital tract infection in prom].the authors conducted a prospective study of the role of lower genital tract infection in ptl and prom in high risk obstetric population with special interest in pathogens colonizing the maternal tract. a total amount of 62 women within 28 to 37 weeks of gestation were enrolled and interviewed. subjects were cultured for gardnerella vaginalis, group b streptococcus, trichomonas vaginalis, neisseria gonorrhoeae, bacterioides fragilis, m. hominis, u. urealyticum, chlamydia trachomatis, candida gal ...19979780503
[estimation of cytological smears in women with gardnerella vaginalis infections of vagina and uterine cervix].among micro-organisms infecting vagina whose dominant genus is gardnerella vaginalis anaerobic bacteria are often present. well-known damaging tissues and cells features of anaerobic bacteria are taken under consideration as factors which are conductive to generating pathological changes in cervical epithelium. the aim of this study was evaluation of cytological smears (pap smear, gram stain and h + e stain) in women with gardnerella vaginalis infections of vagina and uterine cervix. the researc ...19979780509
[colposcopy images of cervix in women with gardnerella vaginalis infection].among micro-organisms infecting vagina whose dominant genus is gv anaerobic bacteria are often present. there are reports that gv infections of vagina and uterine cervix, apart from their well-known negative role in obstetrics practice can play a role in carcinogenic processes of uterine cervix. the aim of the study was to assess characteristic colposcopy images of cervix in women with gardnerella vaginalis infection. the research was carried out on 1180 women hospitalised in the period of 14 mo ...19979780510
cervical cytology in women with bacterial vaginosis.398 symptomatic cases of vaginitis and 43 asymptomatic healthy women were subjected to cervical cytology and microbiological investigations for detection of bacterial vaginosis, g. vaginalis, t. vaginalis, c. albicans and m. hominis. cervical inflammatory changes were reported in 81.4% of cases and 60.4% of controls (p < 0.05) and were significantly associated with bacterial vaginosis and g. vaginalis infection (p < 0.01). cervical inflammation was detected in all cases of trichomonasis.19989805847
prevalence of gardnerella vaginalis in prepubertal males.to determine the rate of occurrence of gardnerella vaginalis in the genital tract and rectum of the asymptomatic prepubertal boy and to examine the effect of circumcision on the rate of recovery.19989811287
impairment of the mucosal immune system: iga and igm cleavage detected in vaginal washings of a subgroup of patients with bacterial vaginosis.the integrity of the immunoglobulins in vaginal washings of patients with bacterial vaginosis was examined to answer the question of the lack of immune response against gardnerella vaginalis cytolysin. clinically diagnosed patients (n=100) were recruited and their vaginal washings examined by western blotting. many showed iga and igm partially or extensively degraded. according to the degradation pattern, the patients were subdivided into 4 subsets, from intact (score 0) to completely degraded i ...19989815222
relationship between maternal group b streptococcal colonization and pregnancy outcome.to examine the association of maternal colonization with group b streptococcus (gbs) with preterm labor and premature rupture of the membranes (prom).19989840551
can a laboratory diagnosis of bacterial vaginosis be made from a transported high vaginal swab using anaerobic culture and microscopy of a wet preparation? 19989849564
phylogenetic analysis of the genus bifidobacterium and related genera based on 16s rdna sequences.the 16s rrna gene sequences were determined for type strains of 21 bifidobacterium species. a phylogenetic tree was constructed using the determined sequences and sequences from dna databases, which contain the sequences of 11 type strains of bifidobacterium species and 11 strains of related genera. all species of the genus bifidobacterium and gardnerella vaginalis atcc 14018 belonged to a cluster phylogenetically distinct from the other genera. the cluster was divided into two subclusters: subc ...19989858460
[the treatment of vaginal infections with macmiror and macmiror complex].the aim of the present study was to examine the therapeutic possibilities of the wide-spectrum medicament macmiror & macmiror complex for the treatment of the vaginal infections.19989859542
[study on the inactivated vaccine of gardnerella vaginalis of fox. vi. experimental development of the inactivated vaccine of gardnerella vaginalis of fox].the aluminum hydroxide gel, propolis and oil adjuvant inactivated vaccines were developed by silecting good immunity, representing principal epidemic serotype i gvf44 of gardnerella vaginalis of fox in civil. the results showed that the aluminum hydroxide gel vaccine in safety was better than propolis and oil adjuvant vaccines, the most optimal inactivated concentration of formalin is 0.1%. efficient immunity doses are 4 billon bacteria/1 ml. there were on any bad influences to occur for fox aft ...19979863213
the growth of gardnerella vaginalis and lactobacillus acidophilus in sorbarod biofilms.sorbarod biofilms were investigated for their suitability in establishing continuous culture biofilms for the study of bacterial vaginosis. two important organisms in the condition, gardnerella vaginalis and lactobacillus acidophilus, were studied. in contrast to growth in broth culture, both organisms were maintained for at least 96 h in a steady state on the biofilms. with g. vaginalis, the haemolytic activity was consistently maintained in the biofilms in contrast to short-term activity in br ...19989879940
gram stain method shows better sensitivity than clinical criteria for detection of bacterial vaginosis in surveillance of pregnant, low-income women in a clinical setting.the purpose of the study is to determine whether the gram stain method is superior to the clinical criteria for the diagnosis of bacterial vaginosis in low-income pregnant women seen in a resident clinic setting. the clinical criteria is the current diagnostic method employed to diagnose bacterial vaginosis.19989894174
[survey of sexually transmitted diseases in the region of rio cuarto].sexually transmitted diseases (std) are acquired mainly through sexual intercourse, being one of the most frequent groups of infectious diseases worldwide and consequently an important public health problem. the aim of this paper was to determine the current state of std and to compare different diagnostic methods in the population studied. a total of 1060 samples from vaginal flows, endocervical material and urethral discharge were studied during 3 years. of the total samples, 583 were positive ...19989922478
assessment of office-based care of sexually transmitted diseases and vaginitis and antibiotic decision-making by obstetrician-gynecologists.survey office-based obstetric-gynecologic practitioners regarding their knowledge of infectious disease care and antibiotic use.19989972486
electron microscopy studies on gardnerella vaginalis grown in conventional and biofilm systems.the cell-wall characteristics of gardnerella vaginalis grown in conventional and biofilm systems were studied by electron microscopy. the gram-positive nature of the cell wall was confirmed. novel cell-wall particles which appeared to be associated with cell division were also identified, particularly in organisms of biofilm origin.19999989650
[chronic bacterial prostatitis]. 199910067117
activation of human immunodeficiency virus type 1 expression by gardnerella vaginalis.bacterial vaginosis (bv) is associated with an increased rate of sexual transmission of human immunodeficiency virus (hiv) type 1, and gardnerella vaginalis is frequently isolated from the genital tracts of women with bv. g. vaginalis lysates were found to significantly stimulate hiv expression in monocytoid cells. stimulation was significantly higher when lysates were heated at 100 degrees c for 5 min but was reduced by treatment with lysozyme or protease. g. vaginalis lysates also activated hi ...199910068588
risk factors for laparoscopically confirmed pelvic inflammatory disease: findings from mumbai (bombay), india.sexually transmitted diseases (stds) are an important cause of pelvic inflammatory disease (pid) but have often not been detected in microbiological studies of indian women admitted to hospital gynaecology wards or private clinics. in this cross sectional study, women living in the inner city of mumbai (bombay) were investigated for socioeconomic, clinical, and microbiological risk factors for pid.199810195052
recurrent bacterial vaginosis and metronidazole resistance in gardnerella vaginalis. 199810195061
clinical and microbiological aspects of vaginitis. 199810218298
in vitro activities of pazufloxacin, a novel injectable quinolone, against bacteria causing infections in obstetric and gynecological patients.t-3762 is an injectable new quinolone with a broad spectrum of antibacterial activity. pazufloxacin (t-3761) is an active form of t-3762. the minimal inhibitory concentrations (mics) of pazufloxacin for 50% of the clinical isolates tested were 3.13 microg/ml for streptococcus agalactiae, 6.25 microg/ml for gardnerella vaginalis, 0.025 microg/ml for escherichia coli, 0.78 microg/ml for pseudomonas aeruginosa, 6.25 microg/ml for peptostreptococcus magnus, 6.25 microg/ml for bacteroides fragilis an ...199910224336
the effects of three nonoxynol-9 preparations on vaginal flora and epithelium.to evaluate the effects of nonoxynol-9 (n-9) on the vaginal flora and epithelium, 48 women (16 in each group) were evaluated by use of quantitative vaginal cultures and colposcopy. at baseline and at 0.5, 4, 24, 48, and 72 h after insertion of one of three n-9 preparations (4% gel [conceptrol], 3.5% gel [advantage-24], or a 28% vaginal contraceptive film). the proportion positive for h2o2+ or h2o2- lactobacilli did not change significantly with any of the preparations, but lactobacilli concentra ...199910395859
bacterial vaginosis-associated microflora isolated from the female genital tract activates hiv-1 expression.alteration of cervicovaginal microbial flora can lead to vaginosis, which is associated with an increased risk of hiv-1 transmission. we recently characterized a soluble hiv-inducing factor (hif) from the cervicovaginal lavage (cvl) samples of women. the goals of this study were to determine the effect of cervicovaginal microflora on hiv-1 expression and to elucidate the relationship between hif activity and microflora. physiologically relevant microorganisms, mycoplasma, diphtheroid-like bacter ...199910421242
bacterial vaginosis: a review.bacterial vaginosis (bv) is a clinically definable condition that is frequently diagnosed in the primary care setting. changes in the nomenclature, causative microorganisms, as well as treatment have caused confusion over the last century. bv is currently viewed as a shift in the normal vaginal ecosystem from a predominance of aerobic bacteria to anaerobic bacteria (soper, 1993), resulting in a malodorous discharge. however, more than one half of clinically diagnosed patients are asymptomatic. r ...199810455564
acid production by vaginal flora in vitro is consistent with the rate and extent of vaginal acidification.perinatally, and between menarche and menopause, increased levels of estrogen cause large amounts of glycogen to be deposited in the vaginal epithelium. during these times, the anaerobic metabolism of the glycogen, by the epithelial cells themselves and/or by vaginal flora, causes the vagina to become acidic (ph approximately 4). this study was designed to test whether the characteristics of acid production by vaginal flora in vitro can account for vaginal acidity. eight vaginal lactobacillus is ...199910496892
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