| detection and characterization of a bacteriocin, putadicin t01, produced by pseudomonas putida isolated from hot spring water. | pseudomonas strains isolated from hot spring water were tested for bacteriocin-like substance (bls) production using a target panel of closely related microorganisms and other gram-positive and gram-negative bacteria. molecular identification was carried out through specific pcr and 16s rna sequence analysis. isolates were identified as brevundimonas diminuta and pseudomonas putida, the latter exhibited antimicrobial activity. pseudomonas putida strains produce an inhibitory substance against ot ... | 2015 | 25556393 |
| [peculiarities of the brevundimonas diminuta gl7aca-acylase quaternary structure formation and obtaining stable enzyme analogues]. | the physicochemical and enzymatic properties of hybrid analogues of the brevundimonas diminuta gl7aca-acylase (brdgia), containing the n-terminal chitin-binding domain of the bacterial chitinase (brdg1a/nmchbd) or the c-terminal oligohistidine sequence (brdgia/h), were studied. an enhanced thermostability level of brdg1a/nmchbd could suggest the stabilizing effect of the chitin-binding domain. an analysis of ph profiles of the enzymatic activity of recombinat brdgia analogues did not reveal sign ... | 2014 | 25507777 |
| [peculiarities of the brevundimonas diminuta gl7aca-acylase quaternary structure formation and obtaining stable enzyme analogues]. | the physicochemical and enzymatic properties of hybrid analogues of the brevundimonas diminuta gl7aca-acylase (brdgia), containing the n-terminal chitin-binding domain of the bacterial chitinase (brdg1a/nmchbd) or the c-terminal oligohistidine sequence (brdgia/h), were studied. an enhanced thermostability level of brdg1a/nmchbd could suggest the stabilizing effect of the chitin-binding domain. an analysis of ph profiles of the enzymatic activity of recombinat brdgia analogues did not reveal sign ... | 2014 | 25434179 |
| comparative investigation of the reaction mechanisms of the organophosphate-degrading phosphotriesterases from agrobacterium radiobacter (opda) and pseudomonas diminuta (oph). | metal ion-dependent, organophosphate-degrading enzymes have acquired increasing attention due to their ability to degrade and thus detoxify commonly used pesticides and nerve agents such as sarin. the best characterized of these enzymes are from pseudomonas diminuta (oph) and agrobacterium radiobacter (opda). despite high sequence homology (>90 % identity) and conserved metal ion coordination these enzymes display considerable variations in substrate specificity, metal ion affinity/preference an ... | 2014 | 25104333 |
| characterization of bacterial community of raw milk from dairy cows during subacute ruminal acidosis challenge by high-throughput sequencing. | four cannulated primiparous holstein dairy cows (84 ± 25 dim) were used in a 2 × 2 crossover experimental design. the two diets contained 40% (low-concentrate diet, or control diet, lc) and 70% (high-concentrate diet, or sara induction diet, hc) concentrate feeds respectively. milk samples were collected on days 17, 18 and 19 of each experimental period. dna was extracted from each milk sample, and pyrosequencing was applied to analyse the milk microbial community. | 2015 | 24961605 |
| the effect of conformational variability of phosphotriesterase upon n-acyl-l-homoserine lactone and paraoxon binding: insights from molecular dynamics studies. | the organophosphorous hydrolase (pte) from brevundimonas diminuta is capable of degrading extremely toxic organophosphorous compounds with a high catalytic turnover and broad substrate specificity. although the natural substrate for pte is unknown, its loop remodeling (loop 7-2/h254r) led to the emergence of a homoserine lactonase (hsl) activity that is undetectable in pte (kcat/km values of up to 2 × 10(4)), with only a minor decrease in pte paraoxonase activity. in this study, homology modelin ... | 2013 | 24352010 |
| production of nano zinc, zinc sulphide and nanocomplex of magnetite zinc oxide by brevundimonas diminuta and pseudomonas stutzeri. | zno (zincite) nanoparticle has many industrial applications and is mostly produced by chemical reactions, usually prepared by decomposition of zinc acetate or hot-injection and heating-up method. synthesis of semi-conductor nanoparticles such as zns (sphalerite) by ultrasonic was previously reported. in this work, high-zinc tolerant bacteria were isolated and used for nano zinc production. among all isolated microorganisms, a gram negative bacterium which was identified as brevundimonas diminuta ... | 2013 | 24206770 |
| engineering v-type nerve agents detoxifying enzymes using computationally focused libraries. | vx and its russian (rvx) and chinese (cvx) analogues rapidly inactivate acetylcholinesterase and are the most toxic stockpile nerve agents. these organophosphates have a thiol leaving group with a choline-like moiety and are hydrolyzed very slowly by natural enzymes. we used an integrated computational and experimental approach to increase brevundimonas diminuta phosphotriesterase's (pte) detoxification rate of v-agents by 5000-fold. computational models were built of the complex between pte and ... | 2013 | 24041203 |
| high-level expression of glutaryl-7-aminocephalosporanic acid acylase from pseudomonas diminuta nk703 in escherichia coli by combined optimization strategies. | in this work, a glutaryl-7-aminocephalosporanic acid acylase (gla) coding gene was cloned from pseudomonas diminuta nk703 which was screened from oilfield. the concerted effects of the expression system, inducing condition and culture medium on the expression of nk703 gla in e. coli were firstly investigated. the best combination was the recombinant e. coli strain of pet-28a+gla/bl21(de3) with 2.0% (w/v) lactose inducing in yt medium at 25°c. then, by optimizing the components of culture medium, ... | 2013 | 23994688 |
| expression and subcellular localization of organophosphate hydrolase in acephate-degrading pseudomonas sp. strain ind01 and its use as a potential biocatalyst for elimination of organophosphate insecticides. | organophosphate hydrolase (oph), the product of an organophosphate-degrading (opd) gene cloned from brevundimonas diminuta, hydrolyses the triester linkage found in neurotoxic organophosphate (op) insecticides and nerve agents. despite the fact that ophs have a broad substrate range, op compounds with a p-s linkage, such as insecticides like acephate, are poor substrates for the enzyme. expression of oph in acephate-utilizing pseudomonas sp. ind01 generated a live biocatalyst capable of degradin ... | 2013 | 23574004 |
| catalytic biofilms on structured packing for the production of glycolic acid. | while structured packing modules are known to be efficient for surface wetting and gas-liquid exchange in abiotic surface catalysis, this model study explores structured packing as a growth surface for catalytic biofilms. microbial biofilms have been proposed as self-immobilized and self-regenerating catalysts for the production of chemicals. a concern is that the complex and dynamic nature of biofilms may cause fluctuations in their catalytic performance over time or may affect process reproduc ... | 2013 | 23412062 |
| evidence for grow-through penetration of 0.2-μm-pore-size filters by serratia marcescens and brevundimonas diminuta. | we find that both brevundimonas diminuta and serratia marcescens can grow through sterilizing grade filter membranes of different membrane polymer compositions. although this passage does not occur on a consistent basis, generation of "grow-through positive" results indicate that grow-through can occur stochastically at basal levels. this observation argues that the following risk mitigation strategies during pharmaceutical aseptic processing are warranted: minimization of processing times, and ... | 2013 | 23385852 |
| pleuritis due to brevundimonas diminuta in a previously healthy man. | brevundimonas diminuta is rarely associated with invasive infections. we report the case of a previously healthy young man with pleural effusion, in which b. diminuta was recovered but incorrectly identified as kingella kingae when it was freshly isolated. consequently, the misidentification resulted in initial treatment failure. the correct identification was achieved through further incubation, sequencing of the 16s rrna gene and ms. | 2013 | 23180480 |
| brevundimonas abyssalis sp. nov., a dimorphic prosthecate bacterium isolated from deep-subsea floor sediment. | a novel gram-negative, aerobic, psychrotolerant, alkali-tolerant, heterotrophic and dimorphic prosthecate bacterium, designated strain tar-001(t), was isolated from deep-sea floor sediment in japan. cells of this strain had a dimorphic life cycle and developed an adhesive stalk at a site not coincident with the centre of the cell pole, and the other type of cell, a swarm cell, had a polar flagellum. colonies were glossy, viscous and yellowish-white in colour. the temperature, ph and salt concent ... | 2013 | 23041635 |
| identification and characterization of novel catalytic bioscavengers of organophosphorus nerve agents. | in an effort to discover novel catalytic bioscavengers of organophosphorus (op) nerve agents, cell lysates from a diverse set of bacterial strains were screened for their capacity to hydrolyze the op nerve agents vx, vr, and soman (gd). the library of bacterial strains was identified using both random and rational approaches. specifically, two representative strains from eight categories of extremophiles were chosen at random. for the rational approach, the protein sequence of organophosphorus h ... | 2013 | 23041042 |
| a further biochemical characterization of drpll the thermophilic lactonase from deinococcus radiodurans. | recently, the cloning of the orf dr0930 from deinococcus radiodurans displaying, as primary activity, a lactonase activity and a promiscuous phosphotriesterase activity was reported. the crystal structure of the resulting recombinant enzyme has been solved, and many mutants have been generated in order to increase the phosphotriesterase activity, with the aim to reach the level of activity of the related ppte from pseudomonas diminuta. in this paper we report an additional biochemical characteri ... | 2013 | 22789107 |
| the importance of accurate microorganism identification in microbial challenge tests of membrane filters. part ii. the comparison of hydrogenophaga pseudoflava attc 33668 and curvibacter sp. atcc 700892 by microbial challenge tests with membrane filters. | the astm 838-05 standard describes a bacteria challenge test procedure based on brevundimonas diminuta (atcc 19146) to verify a 0.2 μm rated sterilizing-grade filter. for process validation procedures a correct identification of the challenge organism is essential. the test strain atcc 700892 repeatedly used for microbial challenge tests was incorrectly named hydrogenophaga pseudoflava but is phylogenetically linked to the genus curvibacter, as shown in part i of this series. based on these stud ... | 2016 | 22767883 |
| hydrolysis of phosphotriesters: a theoretical analysis of the enzymatic and solution mechanisms. | a theoretical study on the alkaline hydrolysis of paraoxon, one of the most popular organophosphorus pesticides, in aqueous solution and in the active site of pseudomonas diminuta phosphotriesterase (pte) is presented. simulations by means of hybrid quantum mechanics/molecular mechanics (qm/mm) potentials show that the hydrolysis of paraoxon takes place through an a(n)d(n) or associative mechanism both in solution and in the active site of pte. the results correctly reproduce the magnitude of th ... | 2012 | 22745111 |
| molecular cloning of brevundimonas diminuta for efficacy assessment of reverse osmosis devices. | brevundimonas diminuta is the test organism specified in the united states environmental protection agency's (usepa) reverse osmosis (ro) treatment device verification protocol. as non-selective growth medium is employed, enumeration of b. diminuta may be impaired due to interference by indigenous heterotrophic bacteria. thus the microbial removal capability of the filtration system may be incorrectly assessed. as these treatment devices are used in emergency situations, the health of the public ... | 2012 | 22717753 |
| molecular dynamics of organophosphorous hydrolases bound to the nerve agent soman. | the organophosphorous hydrolase (oph) from pseudomonas diminuta is capable of degrading extremely toxic organophosphorous compounds with a high catalytic turnover and broad substrate specificity. although the natural substrate for oph is unknown, its triple-mutant h254g/h257w/l303t exhibits a 3 order of magnitude increase in catalytic efficiency and modified stereospecificity toward the most toxic spsc enantiomer of soman. molecular dynamics simulations and binding free-energy calculations have ... | 2007 | 26633227 |