| [epidemiological evaluation of the serological grouping of meningococci]. | use of the precipitation and hemagglutination inhibition tests to determine the serological group of 114 meningococcus strains which cannot be grouped by agglutination slide permitted to establish the serological group of 56% of the strains, this pointing to the greater diagnostic value of these tests. nevertheless, 70% of 307 strains isolated from carriers could not be referred to any of the determinable groups (a, c, x, y, and z). strains (417) isolated from the cerebrospinal fluid of patients ... | 1978 | 106606 |
| gonorrhea: recommended treatment schedules, 1978. | | 1979 | 106717 |
| evaluation of a gonococcal serologic test. | the fluorescent gonorrhea test--heated (fgt-h), an indirect immunofluorescent method for detecting the presence of antigonococcal antibodies in serum, was evaluated in 1,000 women referred to a private clinic for the termination of pregnancy, 202 women treated at a municipal venereal disease clinic, and six women hospitalized with gonococcal bacteremia. the test was positive for 17.7% of private outpatients, even though gonococci were cultured from only 1.1% of these women, and another 5.1% had ... | 1979 | 106719 |
| raised intracranial pressure: its management in neisseria meningitidis meningoencephalitis. | further reduction in morbidity and mortality from bacterial meningitis is unlikely to stem from innovations in antibiotic management. emphasis must therefore be directed toward prevention, more rapid diagnosis, and improved therapy for maintaining the integrity of the cns. raised intracranial pressure is frequently associated with bacterial meningitis. in selected patients with bacterial meningitis and clinical evidence of elevated intracranial pressure, monitoring and aggressive treatment of th ... | 1979 | 106721 |
| purification by affinity chromatography and properties of a beta-lactamase isolated from neisseria gonorrhoeae. | beta-lactamase activity was detected in cell-free preparations obtained from ultrasonic treatment of neisseria gonorrhoeae after growth in liquid medium. crude preparations of beta-lactamase were subjected to affinity chromatography, using several beta-lactam antibiotics as ligands bound to agarose supports. affinity gels produced by coupling 7-aminocephalosporanic acid or 6-aminopenicillanic acid by their amino groups to carboxyl-terminal agarose via a five- to eight-carbon spacer arm proved to ... | 1979 | 106775 |
| in vitro activity of rosamicin, josamycin, erythromycin, and clindamycin against beta-lactamase-nagative and beta-lactamase-positive strains of neisseria gonorrhoeae. | randomly selected strains of beta-lactamase-negative and beta-lactamase-positive neisseria gonorrhoeae were tested by an agar dilution method for susceptibility to rosamicin, josamycin, erythromycin, clindamycin, and penicillin g. rosamicin was more active than erythromycin, which was more active than josamycin or clindamycin; the latter two were similar in their activity. the susceptibility to the macrolide antibiotics and clindamycin was independent of beta-lactamase production, but the penici ... | 1979 | 106776 |
| [detection of healthy carriers of neisseria meningitidis in a sample of the population of the city of messina]. | | 1977 | 106778 |
| [presence of anti-neisseria meningitidis antibodies in the normal population of messina]. | | 1977 | 106779 |
| an assessment of the microcult-gc culture test. | microcult-gc, a miniaturised culture test, was compared with an established selective culture method for laboratory confirmation of gonorrhoea. microcult-gc correlated 94% with the control method in identifying gonococci in male urethral specimens. microcult-gc testing of 130 gonococci-negative vaginal swabs gave only 4.6% false-positive oxidase results, which could probably have been readily refuted by microcult gram stain. the test may be difficult to interpret for rectal specimens and is not ... | 1979 | 106917 |
| pharyngeal colonisation by neisseria gonorrhoeae and neisseria meningitidis in black and white patients attending a venereal disease clinic. | pharyngeal colonisation by neisseria gonorrhoeae and neisseria meningitidis was studies in 2000 patients attending a venereal disease clinic. of these patients, 64% were white and 36% were black. the incidence of gonococcal infections was highest in the period from june to august. the incidence of genital or rectal infections or both was higher in the black patients. pharyngeal colonisation by gonococci was present in 1.3% of the patients. there was no significant associations between pharyngeal ... | 1979 | 106918 |
| inhibition of neisseria gonorrhoeae by normal human saliva. | saliva was found to be a powerful and specific inhibitor of neisseria gonorrhoeae. although 28 other species of bacteria were tested, including neisseria meningitidis, neisseria pharyngis var flava, neisseria lactamica, and neisseria catarrhalis, we failed to find any others sensitive to saliva under similar conditions. the physical properties of the inhibitory substance indicated that it might be salivary alpha-amylase. to test this hypothesis alpha-amylase was extracted from saliva and was sho ... | 1979 | 106919 |
| inhibition of gonococci by a selective medium: disparity between isolates from sexual partners. | | 1979 | 106920 |
| [effects of chemotherapeutic agents on strains of neisseria meningitidis isolated in bohemia during 1970-1976]. | | 1979 | 106970 |
| [observation of changes in sensitivity of neisseria gonorrhoeae to antibiotics in 1966-1975 (author's transl)]. | | 1979 | 106973 |
| penicillin-resistant gonorrhea. | | 1979 | 106997 |
| primary meningococcal conjunctivitis in an infant. | a case of primary meningococcal conjunctivitis in an infant was presented and its treatment is discussed. because of the potential for systemic spread of the infection in the patient, local and systemic therapy was instituted. the current literature on meningococcal conjunctivitis with its potential complications is briefly reviewed. | 1979 | 106998 |
| in vitro antimicrobial activity of piperacillin and seven other beta-lactam antibiotics against neisseria gonorrhoeae and haemophilus influenzae, including beta-lactamase producing strains. | | 1979 | 107152 |
| electron capture gas-liquid chromatographic-mass spectral identification of acids produced by neisseria meningitidis in a defined medium. | the acid metabolites and the cellular fatty acids of three strains of neisseria meningitidis grown in a chemically defined liquid medium were determined with computerized frequency-pulsed electron capture gas-liquid chromatography. five acids not previously reported were subsequently identified: isobutyric, octanoic, decenoic (c10:1), dodecenoic (c12:1), and tetradecenoic (c14:1). these acids were produced during active metabolism and were not detected as cellular constituents. the frequency-pul ... | 1979 | 107187 |
| serotypes of neisseria meningitidis isolated from patients in norway during the first six months of 1978. | during the first 6 months of 1978, 114 strains of neisseria meningitidis isolated from patients in norway were serotyped. among 27 group c strains, type 2 was most common, whereas 82% of the 82 group b isolates did not react with antisera to the standard serotypes 1 to 12. these strains were shown to belong to a new serotype, type 15. also some group a and c strains had the type 15 antigen. investigations on a possible immunoprophylaxis against group b meningococcal disease in norway should acco ... | 1979 | 107188 |
| gonococcal interactions with polymorphonuclear neutrophils: importance of the phagosome for bactericidal activity. | gonococci are capable of attaching to the surface of polymorphonuclear leukocytes (pmn). in this location they resist phagocytosis and are not killed by pmn. to delineate the factors involved in the survival of these gonococci, we investigated the interaction of virulent gonococci, which adhere to cells and resist phagocytosis, and avirulent gonococci, which are phagocytized and killed by pmn. in the presence of serum, both virulent and avirulent gonococci associate equally well with pmn and sti ... | 1978 | 107196 |
| [a case of branhamella catarrahalis pneumonia (author's transl)]. | | 1978 | 107249 |
| differences in attachment antigens of gonococci in reinfection. | on the basis of bacterial typing, pyocin typing, and antibiotic sensitivity tests, two consorts appeared to have been reinfected 34 and 41 days later, respectively, with the same gonococcal strain, suggesting a failure in these patients to develop effective immunity to reinfection. however, these tests do not measure antigens which mediate attachment, a function which may correlate with virulence. when the above infecting strains were retested in an inhibition-of-attachment assay using rabbit go ... | 1979 | 107257 |
| development of antibodies to meningococcal protein and lipopolysaccharide serotype antigens in healthy-carriers. | the nasopharyngeal acquisition of meningococci was followed in healthy military recruits during their primary training. the production of antibodies to meningococcal serotype protein antigens and serotype lipopolysaccharide antigens accompanied the carrier state. bactericidal antibody to protein serotype 2 was generated in response to the carriage of meningococci of low virulence that carried this antigen. | 1979 | 107314 |
| [n. meningitidis carriers in young age groups (author's transl)]. | | 1978 | 107384 |
| [serogroup a n. meningitidis polysaccharide vaccine and serogroup a n. meningitidis carrier state (author's transl)]. | | 1978 | 107385 |
| a trial of minocycline given after exposure to prevent gonorrhea. | in a prospective evaluation of antibiotic prophylaxis against gonorrhea, 1080 men were given 200 mg of oral minocycline or placebo after sexual intercourse with prostitutes in a far eastern port. later, at sea, gonococcal infection was detected in 57 of 565 men given placebo and 24 of 515 men given minocycline (p less than 0.001). minocycline prophylaxis completely prevented infection by gonococci susceptible to 0.75 microgram or less of tetracycline per milliliter, reduced the risk of infection ... | 1979 | 107450 |
| [a patient with meningococcal pericarditis without signs of meningitis]. | | 1979 | 107462 |
| a rapid method for the identification of plasmid desoxyribonucleic acid in bacteria. | | 1978 | 107540 |
| bacteriology of acute pelvic inflammatory disease. suboptimal survival of neisseria gonorrhoeae in a nonbuffered transport system. | in our earlier study, neisseria gonorrhoeae was infrequently isolated while bacteroides fragilis was totally absent from cul-de-sac aspirates of patients with acute pelvic inflammatory disease (pid). twenty additional women were studied to further elucidate the bacteriology of this disease. recovery rate of n. gonorrhoeae from cul-de-sac aspirates collected in selective transport medium (transgrow) was 25 per cent among patients with gonococcal pid, compared to only 8 per cent when specimens wer ... | 1979 | 107802 |
| stability and viability of neisseria gonorrhoeae in various solutions and buffers. | the stability and viability of neisseria gonorrhoeae wp (t4) was tested in a variety of buffers and solutions, many of which are commonly used in gonococcal research. each solution was tested at room temperature for its ability to maintain stability and viability of gonococci in concentrated suspensions and to maintain viability of gonococci in dilute suspensions. the 14 buffers and solutions tested could be divided into four groups based upon these criteria. only a few solutions satisfied all t ... | 1979 | 107855 |
| specificity of dna uptake in genetic transformation of gonococci. | | 1979 | 107953 |
| identification of amino sugars from bacterial lipopolysaccharides by gas chromatography electron impact and chemical ionization mass spectrometry. | amino sugars isolated from lipopolysaccharides of brucella suis, brucella abortus and neisseria gonorrhoeae colony types 1 and 4 were identified using gas chromatography electron impact and chemical ionization mass spectrometry. lipopolysaccharides were obtained by aqueous ether or aqueous phenol extraction. isolated lipopolysaccharides were hydrolyzed in 1% acetic acid followed by hydrolysis of the polysaccharide moiety in 2 nhcl for 6 h at 100 degrees c. amino sugars were first isolated by elu ... | 1978 | 107981 |
| transport of neisseria meningitidis cultures: growth-supporting media and freezing as an alternative. | the abilities of transgrow (tg), thayer-martin (tm), and new york city (nyc) solid media to maintain the viability of 12 strains of neisseria meningitidis under various controlled conditions were assessed. the effects of charcoal impregnation of swabs, temperature, and an enriched co2 atmosphere were examined with holding for up to 21 days. recovery from samples held at 35 degrees c was, in almost all instances, greater than at 22 or 4 degrees c. a strong requirement for added co2 was demonstrat ... | 1979 | 108008 |
| induction of reaginic (ige) gonococcal antibodies in the rat by a common antigen of neisseria gonorrhoeae. | an antigen (zab) common to neisseria gonorrhoeae was prepared by stepwise elution of a crude gonococcal antigen (za) from columns of diethylaminoethyl cellulose employing 0.02 m phosphate buffers, ph 7.6, containing increasing concentrations of sodium chloride. rats immunized with zab produced reaginic (ige) antibody which cross-reacted with za prepared from eight gonococcal strains by the passive cutaneous anaphylaxis (pca) test. heating of the sera at 56 degrees c for 4 h destroyed the pca act ... | 1979 | 108009 |
| isolated gonococcal pulmonary valve endocarditis: diagnosis by echocardiography. | the perplexing clinical course of a 23-year-old black male with isolated gonococcal pulmonary valvular endocarditis is presented. m-mode echocardiography provided the first clue to the presence of pulmonary valvular vegetations and the proper diagnosis. since neisseria gonorrhea appears to have a particular affinity for the pulmonary valve, the presence of isolated pulmonary valvular endocarditis should raise the strong possibility that neisseria gonorrhea is the offending organism. this case re ... | 1979 | 108032 |
| [reevaluation of the effect of certain parameters on the survival of neisseria gonorrhoeae]. | lysis and survival of 4 gonococcal strains were studied under different conditions in the presence of divalent cations and glycerol. the stability and the viability of the gonococcal cells were not enhanced by any of these compounds. | 1979 | 108131 |
| [a simple method for the incubation of gonococcal cultures]. | | 1979 | 108143 |
| vaginal discharge and sexually transmitted diseases. | | 1978 | 108216 |
| metabolism of pyrimidine bases and nucleosides in neisseria meningitidis. | in neisseria meningitidis, uridine, deoxyuridine, cytosine, cytidine, or deoxycytidine could not be used by uracil-requiring mutants as pyrimidine sources. consistent with these findings, only 5-fluorouracil of the different fluoropyrimidine bases and nucleosides showed any inhibitory effect on the growth of four prototrophic strains of n. meningitidis. likewise, only radioactive uracil was readily incorporated into nucleic acids, whereas uptake of radioactive uridine, cytosine, or cytidine coul ... | 1979 | 108255 |
| transformation-derived neisseria gonorrhoeae plasmids with altered structure and function. | plasmid deoxyribonucleic acid from neisseria gonorrhoeae containing a 7.1-kilobase (kb) (4.7-megadalton) penicillinase (pcr) plasmid transformed homogenic gonococci to penicillinase production at a low frequency. about 25% of the penicillinase-producing gonococcal transformants contained pcr plasmids which were either larger or smaller than the 7.1 kb donor plasmid; these pcr plasmids varied in size from 3.45 to 42 kb. some of these altered plasmids differed from the donor plasmid in stability o ... | 1979 | 108261 |
| rapid serotyping of groups a, b, and c meningococci by rocket-line immunoelectrophoresis and co-agglutination. | rocket-line immunoelectrophoresis (r-lie) with antigen containing intermediate gel, and co-agglutination utilising protein a-containing staphylococci coated with specific antibodies, were adapted for serotyping the prototypes of group b meningococci. both were found to have the same specificity as agar gel double diffusion (agdd) but they were more sensitive and more rapid than agdd. r-lie required, like agdd, the extraction of relatively large quantities of bacteria, while the co-agglutination ... | 1979 | 108320 |
| comparative immunogenicity of vaccines prepared from capsular polysaccharides of group c neisseria meningitidis o-acetyl-positive and o-acetyl-negative variants and escherichia coli k92 in adult volunteers. | three structurally and antigenically similar capsular polysaccharides, two derived from group c neisseria meningitidis (o-acetyl-positive and o-acetyl-negative variants) and one from escherichia coli k92, which cross-reacts with polysaccharide from group c n. meningitidis, were compared for their ability to induce anticapsular and bactericidal antibodies to group c n. meningitidis in adult volunteers. all three vaccines elicited group c-specific serum antibodies. the vaccine derived from the o-a ... | 1979 | 108338 |
| studies on gonococcus infection. xvii. iga1-cleaving protease in vaginal washings from women with gonorrhea. | vaginal washings from women attending a veneral disease clinic were examined for the presence of protease that cleaved iga subclass 1 (iga1). in a crude assay, vaginal washings cleaved [125i]iga1 in 19 of 25 specimens from individuals from whom neisseria gonorrhoeae were cultivated. forty-six specimens from 104 women whose cultures were negative for n. gonorrhoeae also cleaved [125i]iga1. vaginal washings from six of six women with culture-proven gonorrhea cleaved [125i]iga1 into low-molecular-w ... | 1979 | 108339 |
| complement-enhanced immunity to infection with neisseria gonorrhoeae in mice. | subcutaneous chambers were implanted in mice, injected with neisseria gonorrhoeae, and supplemented with complement as a model for studying the immunogenicity and strain diversity of n. gonorrhoeae. immunotypic resistance to n. gonorrhoeae in immunized mice was significantly (p less than 0.01) increased by injection of exogenous guinea pig complement into the host before challenge with gonococci. by using this model to test gonococcal isolates from various geographical areas, two highly immunoge ... | 1979 | 108344 |
| [anti-neisseria meningitidis antibodies, groups a and c, in individuals from urban and rural regions of argentina]. | | 1978 | 108502 |
| penicillinase-producing neisseria gonorrhoeae. new york city, 1976-1978. | | 1979 | 108637 |
| [diagnosis and therapy of gonorrhea--newer aspects]. | | 1979 | 108866 |
| [biological and chemical characteristics of allergen preparations from neisseria (n. meningitidis, n. gonorrhoeae, n. perflava)]. | various methods of isolating allergen fractions from n. meningitidis, n. gonorrhoeae and n. perflava were tested. the biological activity of the preparation was found to depend on the method of its production, which determined its chemical composition. when gonococcal and meningococcal allergens and n. perflava allergen were used in skin tests, cross reactions were observed. nevertheless, as the intensity and size of skin reaction was much greater when a homologous preparation was administered, ... | 1979 | 108881 |
| the interaction of penicillin and chloramphenicol against meningococci in vitro. | combinations of penicillin and chloramphenicol are frequently used initially in the treatment of bacterial meningitis. the simultaneous effects against meningococci of these two drugs were examined in vitro in a chemically defined, proteinfree medium. the investigation was performed with different combinations of the antibiotics, including optimal concentrations. in most instances, penicillin and chloramphenicol seemed to have an additive, but not an iso-additive, effect. no antagonism was found ... | 1979 | 108918 |
| electron microscopical study of neisseria meningitidis releasing various amounts of free endotoxin. | a study has been made of the ultrastructure of four strains of neisseria meningitidis which liberate varying amounts of free endotoxin in a chemically-defined, protein-free medium. the two strains which did not release detectable or only sparse amounts of free, filtrable endotoxin were rather uniform in cell size. their cells appeared to be intact and showed a low tendency to aggregate, in addition cells of these strains showed only sporadic loose, trilaminar membranes and blebs, and free membra ... | 1979 | 108919 |
| nonenteric gram negatives, 1976. | | 1979 | 109001 |
| medical therapy of metastatic meningococcal endophthalmitis. | we treated a 15-year-old girl who had meningococcal meningitis and metastatic endophthalmitis with large doses of intravenously administered penicillin g. the patient promptly improved with this therapy, and the visual axis completely cleared during the subsequent five months. | 1979 | 109007 |
| new options for diagnosis and control of gonorrheal urethritis in males using uncentrifuged first voided urine (fvu) as a specimen for culture. | | 1979 | 109017 |
| neisseria meningitidis and neisseria gonorrhoeae bacteremia associated with c6, c7, or c8 deficiency. | we summarize data from 24 previously described or newly diagnosed cases of homozygous deficiency of the sixth, seventh, or eighth components of complement. thirteen of 24 patients had at least one episode, and usually two or more episodes of neisseria meningitidis or neisseria gonorrhoeae bacteremia, or both. deficiency of c6, c7, or c8 is a meaningful risk factor for repeated neisserial bacteremia; conversely, hemolytic complement studies are indicated in patients who develop recurrent neisseri ... | 1979 | 109025 |
| complement, serum bactericidal activity, and disseminated gram-negative infection. | | 1979 | 109027 |
| meningococcal conjunctivitis. | meningococcal conjunctivitis is typically described as an acute purulent infection. an atypical case of mild catarrhal conjunctivitis occurred in a 19-year-old college student. the meningococci were identified as neisseria meningitidis, group a, and were isolated from the throats of the patient and her roommate. the conjunctivitis responded rapidly to treatment with sodium sulfacetamide, and it was not treated systemically. a short review of the literature of meningococcal conjunctivitis is pres ... | 1979 | 109067 |
| sensitivity of neisseria gonorrhoeae to penicillin in west germany. | the sensitivity of gonococci to penicillin in germany has largely decreased in the last 22 years. no significant decrease has occurred, however, in the last three years, but the results show that a slight tendency to decrease may be persisting. | 1979 | 109164 |
| preservation of neisseria gonorrhoeae by the gelatin-disc method. | studies of neisseria gonorrhoeae are difficult to perform because of the organism's poor survival in vitro. to solve this problem we tried to preserve the organism by a gelatin-disc method. the rate of survival and changes of variations in some biochemical properties of eight strains of n. gonorrhoeae were followed for three years. these studies proved that preservation was satisfactory with only a 1/10 reduction of the living cells. another trial showed that the organism survived for over six m ... | 1979 | 109165 |
| national research council spends $6 million a year on health-related research. | | 1979 | 109180 |
| complex of meningococcal group b polysaccharide and type 2 outer membrane protein immunogenic in man. | a noncovalent complex of meningococcal group b polysaccharide and type 2 outer membrane protein has been characterized and its potential as a vaccine against group b meningococcal disease investigated. the polysaccharide component was found to have a partition coefficient, k(d), of 0.34 on sepharose cl-4b in the presence of sodium deoxycholate. the protein consisted of four to five major proteins including the principal outer membrane protein. hydrophobic binding between the protein and polysacc ... | 1979 | 109466 |
| antibody-dependent cell-mediated antibacterial activity of human mononuclear cells. i. k lymphocytes and monocytes are effective against meningococi in cooperation with human imune sera. | in cooperation with human heat-inactivated antisera from adults immunized with group c meningococcal polysaccharide, normal human peripheral blood mononuclear cells significantly decreased the viability of group c meningococci (mgc) in vitro. k lymphocytes (null cells) and monocytes, (but not t or b lymphocytes) were capable of effecting antibody-dependent cell-mediated (adc) antibacterial activity in this system. the degree to which meningococcal viability was decreased was a function of the le ... | 1979 | 109572 |
| further characterization of responses of infants and children to meningococcal a polysaccharide vaccine. | the responses to 10 or 50 microgram doses of meningococcal group a polysaccharide vaccine were evaluated in infants and children. although the 50 microgram dose was shown to be more effective in the induction of detectable anti-a antibody in vaccinees of all ages, only 6.5% (7/108) of infants smaller than or equal to 12 months demonstrated a "protective level" (greater than or equal to 2 mug/ml antibody protein) at 28 or 35 days postinoculation. unexpected variations in the response to different ... | 1979 | 109592 |
| the gonococcus and the toilet seat. | | 1979 | 109754 |
| haemophilus influenzae sepsis in a splenectomized child. | | 1979 | 109765 |
| specificity of dna uptake in bacterial transformation. | | 1979 | 109770 |
| penicillinase producing gonococci. | | 1979 | 109799 |
| the emergence of antimicrobial resistance in new zealand. | | 1979 | 109800 |
| neisseria gonorrhoeae: physiology and metabolism. | | 1979 | 109930 |
| [comparative efficiency of "transport" culture media for detecting meningococci]. | | 1979 | 109990 |
| meningitis due to neisseria meningitidis, group y. | | 1979 | 110137 |
| effect of medium temperature on recovery of gonococci. | the temperature of the isolation medium exerted no significant effect on the rate of positive cultures for neisseria gonorrhoeae. however, significantly more colonies were obtained on ambient-temperature medium than on cold (4c) medium. a small number of specimens (six) failed to grow on cold medium but grew on ambient-temperature medium. viable counts showed essentially no effect of medium temperature on the nondiscriminating strain, a 90% reduction on the cold medium for the cold-intolerant st ... | 1979 | 110141 |
| meningococcal colonization and infection in children and their household contacts. | a bacteriologic survey was performed to estimate the prevalence and duration of meningococcal carriage in children in montreal, canada. infants and children with proven meningococcal infection, or with asymptomatic meningococcal nasopharyngeal (np) carriage, and their household contacts, were also studied to define communicability. n. meningitidis was present in 30 (2.4%) of the np cultures from 1238 asymptomatic infants and children in this civilian population during a non-epidemic period. meni ... | 1979 | 110143 |
| reversible adrenocorticol insufficiency in fulminant meningococcemia. | a 13-year-old girl who had been well previously was admitted with fulminant meningococcemic purpura. her plasma cortisol level was only 3 micrograms/dl and failed to rise after cosyntropin administration. maintenance doses of corticosteroid were administered. as her infection cleared her adrenal glands were restimulated and shown to have near normal response. replacement doses of corticosteroids should be given to patients with fulminant meningococcemia until adrenal cortical insufficiency can b ... | 1979 | 110279 |
| antibody to an artificial disaccharide antigen cross-reactive with neisseria gonorrhoeae lipopolysaccharide. | an artificial antigen was prepared from 4-o-beta-i-galactopyranosyl-d-glucose (lactose) and 8-ethoxycarbonyloctanol. covalent attachment to bovine serum albumin provided an antigen that elicited antilactose antibody in rabbits and goat. these antibodies were active against neisseria gonorrhoeae lipopolysaccharide in passive hemagglutination tests. the same antibody agglutinated cells of streptococcus faecalis, strain n, and precipitated the lactose-containing cell wall diheteroglycan of this org ... | 1979 | 110427 |
| investigation of females for gonorrhoea. | | 1978 | 110479 |
| studies on gonococcus infection. xviii. 125i-labeled peptide mapping of the major protein of the gonococcal cell wall outer membrane. | the major outer membrane proteins from 10 gonococcal strains were examined after 125i-labeling of the proteins as single bands resolved by polyacrylamide gel electrophoresis in sodium dodecyl sulfate. these 125i-proteins were then treated with either trypsin or alpha-chymotrypsin, and the resultant 125i-peptides were visualized by autoradiography after two-dimensional electrophoretic and chromatographic separation on thin-layer cellulose sheets. several 125i-peptides were present in all the majo ... | 1979 | 110681 |
| pili of neisseria meningitidis: effect of media on maintenance of piliation, characteristics of pili, and colonial morphology. | in contrast to information in the literature which indicates that meningococci rapidly lose pili upon cultivation in vitro, we found that piliation of meningococci could be maintained in vitro for 15 or more passages. pili were present on all eight isolates tested, whether from asymptomatic carriers or from subjects with meningococcal disease. complete loss of piliation occurred in the same two strains on two of the three media tested. on one medium (thayer-martin medium with supplement b), ther ... | 1979 | 110692 |
| strain differentiation of neisseria gonorrhoeae by reverse passive hemagglutination. | a reverse passive hemagglutination test that utilizes human erythrocytes coated with antibody to gonococci was developed to distinguish differences among 11 strains of neisseria gonorrhoeae. different rabbits were immunized with each strain of gonococcus. antibody was purified by passing antiserum over an immunoadsorbent column containing homologous cell walls trapped in a cross-linked polyacrylamide gel. antibody, after absorption with n. meningitidis, was used for coating 11 individual suspens ... | 1979 | 110695 |
| neisseria meningitidis infection in mice: influence of iron, variations in virulence among strains, and pathology. | the influence of iron on neisseria meningitidis infection in c-57 mice was examined. iron sulfate, ferric ammonium citrate, and iron sorbitol citrate all proved to be too toxic for use as infection-enhancing agents. iron dextran displayed an extremely low toxicity, enhanced infection in a dose-dependent manner, and resulted in infection enhancement factors approaching 10(9) for virulent strains of n. meningitidis. fatal iron dextran-enhanced infection was shown to be reversible by in vivo chelat ... | 1979 | 110703 |
| [demonstration of neisseria meningitides in the urogenital tract]. | the occurence of neisseria meningitidis in the urogenital tract is a rarity but nevertheless this may lead to a false diagnosis of gonorrhea, even when the thayer-martin-selective-medium is used and oxidase-reaction is performed. this is demonstrated in our case. a complete diagnosis, including sugar-fermentation, is necessary, when gram-negative diplococci are isolated from urogenital swabs. in the case neisseria meningitidis is isolated, it is not required by law to report it to the public hea ... | 1979 | 110728 |
| genetic exchange mechanisms in neisseria gonorrhoeae. | there are two mechanisms for genetic exchange in neisseria gonorrhoeae. plasmid deoxyribonucleic acid can be transferred by conjugation, which is dependent on the presence of a 24.5-megadalton plasmid in the donor cell. we have shown that chromosomal deoxyribonucleic acid can be exchanged between all colonial variants by transformation, but not by conjugation. in the nonpiliated variants, however, this exchange was dependent on the presence of the 24.5-megadalton plasmid in the recipient cell. | 1979 | 110768 |
| genetic basis for colonial variation in neisseria gonorrhoeae. | when the piliated colony types of neisseria gonorrhoeae, which predominate in recent isolates, were nonselectively subcultured in vitro, they gave rise to large numbers of nonpiliated, avirulent colonial variants. evidence is presented to show that most of this variation occurs after active growth has ceased and that the variation is sensitive to the action of deoxyribonuclease. we suggest that this variation is a result of transformation. a second variation in colonial morphology involved diffe ... | 1979 | 110769 |
| hen fluorescein-labeled gonococcal lipopolysaccharide antibody in the delayed fluorescent antibody technique for the confirmation of neisseria gonorrhoeae. | a fluorescent antibody reagent (termed anti-lps conjugate) was prepared from sera obtained from hens immunized with gonococcal r-type lipopolysaccharide. the reagent was absorbed with formalin-treated cells of neisseria meningitidis. the anti-lps conjugate gave uniform brilliant staining of neisseria gonorrhoeae with little background fluorescence, thus making interpretation and reading of fluorescence simple. the conjugate did not significantly stain cultures of n. meningitidis, neisseria lacta ... | 1979 | 110824 |
| evaluation of two transport systems for gonorrhea cultures. | the ability of the transgrow and jembec systems to yield positive cultures when transported to a central laboratory was compared. there were no significant differences in the recovery rates of the two systems. statistically significant decreases in recovery rate were noted when each system was compared with the traditional plate-candle jar technique. | 1979 | 110827 |
| nuclease enhancement of specific cell agglutination in a serodiagnostic test for neisseria gonorrhoeae. | antiserum to a purified type r lipopolysaccharide antigen isolated from neisseria gonorrhoeae was used in a slide agglutination test and compared with conventional carbohydrate utilization and fluorescent antibody tests to confirm the identity of laboratory cultures classified as typical or "atypical" n. gonorrhoeae. cultures of corynebacterium vaginalis, n. meningitidis, n. catarrhalis, n. sicca, and n. lactamicus were also tested in the slide agglutination procedure. the addition of both deoxy ... | 1979 | 110830 |
| serogroup identification of meningococci by a modified antiserum agar method. | modifications in the antiserum agar method for serogroup identification of meningococci, which reduce the amount of group-specific antisera required and increase long-term storage of prepoured antiserum agar plates, are described. | 1979 | 110832 |
| antibodies to neisseria gonorrhoeae: a study of the urethral exudates of 232 men. | the results of a study of the antibodies that react with neisseria gonorrhoeae in the urethral exudates of 232 men who attended a clinic of the department of sexually transmitted diseases at glasgow, scotland, are presented. of 132 men with gonorrhea, antibodies to the gonococcus were identified by use of an indirect immunofluorescent antibody test in 129 (98%): iga antibody was found in all 129 (98%), igg antibody in 119 (90%), and igm antibody in 64 (49%). iga antibody to the gonococcus was fo ... | 1979 | 110889 |
| molecular characterization of a small haemophilus influenzae plasmid specifying beta-lactamase and its relationship to r factors from neisseria gonorrhoeae. | the ampicillin-resistant haemophilus influenzae strain ve445 which caused purulent meningitis and septicaemia in a newborn child in germany contained a 4.4 megadalton (mdal) plasmid (pve445) and produced a tem type beta-lactamase. the transformation to ampicillin resistance of a sensitive escherichia coli strain with isolated pve445 dna proved that the structural gene for the beta-lactamase resided on this plasmid genome. molecular dna-dna hybridization studies and electron microscope dna hetero ... | 1979 | 110907 |
| detection of gonococcal antigens in urine by radioimmunoassay. | a method of detecting gonococcal antigens by solid-phase radioimmunoassay with radioactively labelled antibody is described. a specificity test has been developed that enables this method to be used to detect gonococcal antigens in urine sediments. when sediments from samples of urine from male patients with gonorrhoea were tested, 31 (74%) of 42 gave positive results, clearly distinguishing them from sediments from urine samples from men with non-specific urethritis, none of which was positive. ... | 1979 | 110935 |
| inhibitors in urine of radioimmunoassay for the detection of gonococcal antigens. | several substances in urine were found to inhibit the radioimmunoassay of added gonococcal antigens. the supernatants of two-thirds of urine samples from male patients with either gonorrhoea or non-specific urethritis (nsu) were inhibitory. the inhibition caused by many, but not all, samples was reduced or completely abolished by the addition of soybean trypsin inhibitor (sti); sti-sensitive inhibition is thought to be due to proteolytic enzymes, probably from pus cells. their inhibitory effect ... | 1979 | 110936 |
| infection of artificial air pouches in the connective tissue of mice with neisseria gonorrhoeae. | artificial air pouches in the connective tissue of mice were evaluated as a means of studying neisseria gonorrhoeae infections. animals inoculated with type-1 n. gonorrhoeae cells developed an infection characterised by infiltration of polymorphonuclear leucocytes. viable cocci could be recovered from the air pouches for up to 10 days after infection and intracellular cocci were evident in electronmicrographs within connective-tissue fibroblasts for at least 35 days, indicating that a persistent ... | 1979 | 110937 |
| [a study of the serological response of sudanese children to three associated immunizations (measles, tetanus, meningococcal a meningitis) (author's transl)]. | the authors first emphasize the efficiency and economic value of associated immunizations in developping countries. they report the first data collected in a campaign of immunization with a single injection of an extemporaneous mixture of antimeasles, antitetanus and antimeningococcal meningitis vaccines. this campaign took place in the republic of suddan from may 1976 to july 1977 and concerned 87 children. the specifications of each vaccine as well as the procedures of vaccination and serologi ... | 1979 | 110997 |
| cefoxitin as a single-dose treatment for urethritis caused by penicillinase-producing neisseria gonorrhoeae. | gonococci that resist standard penicillin regimens by production of a penicillinase are now well established in certain areas of the world. because cefoxitin, a semisynthetic cephamycin, resists gonococcal penicillinase in vitro, we compared procaine penicillin g and cefoxitin in treatment of gonorrhea in an area where 40 per cent of isolates produce penicillinase. one hundred and seven men with culture-proved gonococcal urethritis were given a single dose of either procaine penicillin g, 4.8 mi ... | 1979 | 111119 |
| office microbiology. | | 1979 | 111268 |
| meningitis and bacteremia due to neisseria meningitidis: clinical and laboratory findings in 69 cases from orebro county, 1965 to 1977. | the records for 69 patients with meningococcal disease during 13 years in orebro county were reviewed. the mean incidence during this period was 2.0/100 000 persons/year. the age group less than 1 year had the highest incidence, 15/100 000 infants/year. the mortality was 7.2% and sequelae were found in 13% of the patients. four factors were significantly more frequent in the 7 patients with psycho-neurological sequelae: (1) age greater than 40 years; (2) a condition prior to meningococcal diseas ... | 1979 | 111345 |
| [renewed appearance of penicillinase-forming neisseria gonorrhoeae in germany]. | | 1979 | 111433 |
| [extragenital localization of a gonorrheal infection]. | | 1979 | 111434 |
| [acute meningococcal infection (author's transl)]. | authors report 49 patients bacteriologicallyly diagnosed of acute meningococcal infection collected during a 12 months period out of a series of 76 cases diagnosed on clinical grounds. "n. meningitidis" was found in 18 blood and 43 csf cultures. 31 cases were of the b-group, one was a-group and 17 were not typed. all of them were sulphamide resistant. hyperthermia with vomiting, cephalea, arthralgia and seizures were the initial symptoms. all patients showed pettechiae, purpura and/or ecchymoses ... | 1979 | 111589 |
| dihydrofolate reductase from neisseria sp. | members of the genus neisseria are relatively nonsusceptible to trimethoprim, an inhibitor of dihydrofolate reductase. for example, the minimal inhibitory concentration (mic) of trimethoprim for n. gonorrhoeae ranges from 2 to 70 mug/ml, whereas the mic for escherichia coli is 0.2 mug/ml or less. in an effort to understand this difference, dihydrofolate reductase was partially purified from five neisseria species and compared with the enzyme from e. coli. n. gonorrhoeae dihydrofolate reductase w ... | 1979 | 111611 |
| in vitro activity of hr 756, a new cephalosporin, against neisseria gonorrhoeae. | the in vitro activity of hr 756 was compared with penicillin, cefamandole, cefoxitin, and tetracycline against neisseria gonorrhoeae. a total of 192 randomly selected isolates (of which 23 had minimal inhibitory concentrations of >/=0.5 mug/ml for penicillin) and three beta-lactamase-producing isolates were tested. hr 756 was the most active antibiotic tested, with more than 90% of the isolates inhibited by 0.008 mug/ml and all inhibited by 0.25 mug/ml. | 1979 | 111612 |