| utilization of hexamethylenetetramine (urotropine) by bacteria and yeasts. | a slow growing bacterial population able to utilize hexamethylelenetetramine (urotropine) as sole source of carbon, nitrogen and energy was isolated from soil. from this crude enrichment culture two bacteria were isolated and identified as brevundimonas diminuta and a phyllobacterium sp. by sequencing of 16s ribosomal dna. these bacteria also grew on urotropine but at a lower rate than the enrichment culture. addition of glucose to the latter resulted in growth of some yeasts that overgrew the b ... | 2007 | 17043911 |
| bacterial diversity in aortic aneurysms determined by 16s ribosomal rna gene analysis. | aortic aneurysms are common vascular conditions that cause considerable morbidity and mortality. understanding of the mechanisms involved in the pathogenesis of the condition remains limited. recently, infection has been suggested as possible contributor in the development of the disease. the aim of the present study was to examine aortic aneurysms for the presence of bacterial dna using polymerase chain reaction (pcr) targeting the 16s ribosomal rna (rrna) gene, followed by cloning and sequenci ... | 2006 | 17098542 |
| brevundimonas terrae sp. nov., isolated from an alkaline soil in korea. | a gram-negative, rod-shaped, brevundimonas-like bacterial strain, ksl-145(t), was isolated from an alkaline soil in korea and subjected to a polyphasic taxonomic investigation. strain ksl-145(t) grew optimally at ph 7.5-8.0 and 30 degrees c without nacl. it was characterized chemotaxonomically as containing q-10 as the predominant ubiquinone and c(18 : 1)omega7c and c(16 : 0) as the major fatty acids. the dna g+c content was 61.8 mol%. a phylogenetic analysis based on 16s rrna gene sequences sho ... | 2006 | 17158998 |
| case report: infective endocarditis caused by brevundimonas vesicularis. | there are few reports in the literature of invasive infection caused by brevundimonas vesicularis in patients without immunosuppression or other predisposing factors. the choice of antimicrobial therapy for bacteremia caused by the pathogen requires more case experience to be determined. | 2006 | 17194310 |
| isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments. | in assessing the bacterial populations present in spacecraft assembly, spacecraft test, and launch preparation facilities, extremophilic bacteria (requiring severe conditions for growth) and extremotolerant bacteria (tolerant to extreme conditions) were isolated. several cultivation approaches were employed to select for and identify bacteria that not only survive the nutrient-limiting conditions of clean room environments but can also withstand even more inhospitable environmental stresses. due ... | 2007 | 17308177 |
| activation of the binuclear metal center through formation of phosphotriesterase-inhibitor complexes. | phosphotriesterase (pte) from pseudomonas diminuta is a binuclear metalloenzyme that catalyzes the hydrolysis of organophosphate nerve agents at rates approaching the diffusion-controlled limit. the proposed catalytic mechanism postulates the interaction of the substrate with the metal center and subsequent nucleophilic attack by the bridging hydroxide. x-band epr spectroscopy was utilized to monitor the active site of mn/mn-substituted pte upon addition of two inhibitors, diisopropyl methyl pho ... | 2007 | 17315951 |
| a new phosphotriesterase from sulfolobus acidocaldarius and its comparison with the homologue from sulfolobus solfataricus. | the phosphotriesterase pte, identified in the soil bacterium pseudomonas diminuta, is thought to have evolved in the last several decades to degrade the pesticide paraoxon with proficiency approaching the limit of substrate diffusion (k(cat)/k(m) of 4 x 10(7)m(-1)s(-1)). it belongs to the amidohydrolase superfamily, but its evolutionary origin remains obscure. the enzyme has important potentiality in the field of the organophosphate decontamination. recently we reported on the characterization o ... | 2007 | 17337320 |
| quorum-quenching microbial infections: mechanisms and implications. | | 2007 | 17360274 |
| crystal structures of human carboxylesterase 1 in covalent complexes with the chemical warfare agents soman and tabun. | the organophosphorus nerve agents sarin, soman, tabun, and vx exert their toxic effects by inhibiting the action of human acetylcholinesterase, a member of the serine hydrolase superfamily of enzymes. the current treatments for nerve agent exposure must be administered quickly to be effective, and they often do not eliminate long-term toxic side effects associated with organophosphate poisoning. thus, there is significant need for effective prophylactic methods to protect at-risk personnel from ... | 2007 | 17407327 |
| evaluation of the colorimetric vitek 2 card for identification of gram-negative nonfermentative rods: comparison to 16s rrna gene sequencing. | ninety strains of a collection of well-identified clinical isolates of gram-negative nonfermentative rods collected over a period of 5 years were evaluated using the new colorimetric vitek 2 card. the vitek 2 colorimetric system identified 53 (59%) of the isolates to the species level and 9 (10%) to the genus level; 28 (31%) isolates were misidentified. an algorithm combining the colorimetric vitek 2 card and 16s rrna gene sequencing for adequate identification of gram-negative nonfermentative r ... | 2007 | 17507509 |
| adhesion and viability of waterborne pathogens on p-dadmac coatings. | the attachment of waterborne pathogens onto surfaces can be increased by coating the surfaces with positive charge-enhancing polymers. in this paper, the increased efficacy of polydiallyldimethylammonium chloride (p-dadmac) coatings on glass was evaluated in a parallel plate flow chamber with the use of waterborne pathogens (raoultella terrigena, escherichia coli, and brevundimonas diminuta). p-dadmac coatings strongly compensated the highly negative charges on the glass surface and even yielded ... | 2008 | 17570712 |
| cloning of a novel aldo-keto reductase gene from klebsiella sp. strain f51-1-2 and its functional expression in escherichia coli. | a soil bacterium capable of metabolizing organophosphorus compounds by reducing the p s group in the molecules was taxonomically identified as klebsiella sp. strain f51-1-2. the gene involved in the reduction of organophosphorus compounds was cloned from this strain by the shotgun technique, and the deduced protein (named akr5f1) showed homology to members of the aldo-keto reductase (akr) superfamily. the intact coding region for akr5f1 was subcloned into vector pet28a and overexpressed in esche ... | 2007 | 17575004 |
| crystallization and preliminary x-ray diffraction analysis of the hyperthermophilic sulfolobus solfataricus phosphotriesterase. | organophosphates constitute the largest class of insecticides used worldwide and some of them are potent nerve agents. consequently, organophosphate-degrading enzymes are of paramount interest as they could be used as bioscavengers and biodecontaminants. phosphotriesterases (ptes) are capable of hydrolyzing these toxic compounds with high efficiency. a distant and hyperthermophilic representative of the pte family was cloned from the archeon sulfolobus solfataricus mt4, overexpressed in escheric ... | 2007 | 17620708 |
| reusability of entrapped cells of pseudomonas diminuta for production of 7-aminocephalosporanic acid. | entrapped cells of p. diminuta were used for the production of 7-aminocephalosporanic acid (7-aca), a key intermediate required for the production of most of the clinically used cephalosporin derivatives, i.e., semisynthetic cephalosporins. the repeated batch production of 7-aca with entrapped cells of p. diminuta in different carriers were carried out for six cycles at optimal conditions. it was found that 33% , 38%, and 47% of activity was lost with chitosan, gelatin, and agar, respectively as ... | 2007 | 17625270 |
| an extremely oligotrophic bacterium, rhodococcus erythropolis n9t-4, isolated from crude oil. | rhodococcus erythropolis n9t-4, which was isolated from crude oil, showed extremely oligotrophic growth and formed its colonies on a minimal salt medium solidified using agar or silica gel without any additional carbon source. n9t-4 did not grow under co(2)-limiting conditions but could grow on a medium containing nahco(3) under the same conditions, suggesting that the oligotrophic growth of n9t-4 depends on co(2). proteomic analysis of n9t-4 revealed that two proteins, with molecular masses of ... | 2007 | 17675378 |
| microbial production of 7-amino-cephalosporanic acid and new generation cephalosporins (cephalothin) by different processing strategies. | the development of beta-lactam antibiotics has been a continuous battle of the design of new compounds to withstand inactivation by the ever-increasing diversity of beta-lactamases. semisynthetic cephalosporins like cephalothin were synthesized from 7-amino-cephalosporanic acid (7-aca), and thiophene-2-acetic acid using cephalosporin-c acylase enzyme was studied. the production of cephalosporin-c acylase by pseudomonas diminuta was used and the growth kinetics studied. the optimum condition of e ... | 2007 | 17701482 |
| bismuth dimercaptopropanol (bisbal) inhibits the expression of extracellular polysaccharides and proteins by brevundimonas diminuta: implications for membrane microfiltration. | a 2:1 molar ratio preparation of bismuth with a lipophilic dithiol (3-dimercapto-1-propanol, bal) significantly reduced extracellular polymeric substances (eps) expression by brevundimonas diminuta in suspended cultures at levels just below the minimum inhibitory concentration (mic). total polysaccharides and proteins secreted by b. diminuta decreased by approximately 95% over a 5-day period when exposed to the bismuth-bal chelate (bisbal) at near mic (12 microm). fourier-transform infrared spec ... | 2008 | 17705249 |
| [cloning and expression of variants of the glutaryl-7-aminocephalosporic acid acylase of the bacterium brevundimonas diminuta in escherichia coli cells]. | the gene coding for glutaryl-7-aminocephalosporic acid acylase (gl7aca acylase) of the bacterium brevundimonas diminuta (brdgl7aca), a commercial enzyme widely used in modem biocatalytic technologies for manufacture of b-lactam antibiotics, was cloned. efficient expression systems for producing a "native" recombinant brdgl7aca and its analogs modified by attaching affinity groups--the chitin-binding domain of chitinases a1 and hexahistidine sequence--were designed. it was demonstrated that both ... | 2007 | 17929575 |
| purification and immobilization of recombinant variants of brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in escherichia coli cells. | modified chitin-binding domain (chbd) from bacillus circulans chitinase a1 with w42f mutation in chitin-binding site was genetically fused to different positions within alpha-subunit of glutaryl-7-aminocephalosporanic acid acylase (gla) gene. hybrid proteins were efficiently expressed in e. coli cells as soluble, enzymatically active and correctly processed holoenzymes. chbd-gla fusions were easily affinity purified on chitin column by changing the salt concentration of binding and elution buffe ... | 2008 | 17963935 |
| optimization of ascorbic acid-2-phosphate production from ascorbic acid using resting cell of brevundimonas diminuta. | with the aim to produce ascorbic acid-2-phosphate (asa-2-p) from l-ascorbic acid (asa, vitamin c), nine bacteria conferring the ability to transform asa to asa-2-p were isolated from soil samples alongside known strains from culture collections. most isolates were classified to the genus brevundimonas by 16s phylogenetic analysis. among them, brevundimonas diminuta kacc 10306 was selected as the experimental strain because of its the highest productivity of asa-2-p. the optimum set of conditions ... | 2007 | 18051298 |
| effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation. | spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ... | 2008 | 18083857 |
| effect of shadowing on survival of bacteria under conditions simulating the martian atmosphere and uv radiation. | spacecraft-associated spores and four non-spore-forming bacterial isolates were prepared in atacama desert soil suspensions and tested both in solution and in a desiccated state to elucidate the shadowing effect of soil particulates on bacterial survival under simulated martian atmospheric and uv irradiation conditions. all non-spore-forming cells that were prepared in nutrient-depleted, 0.2-microm-filtered desert soil (dse) microcosms and desiccated for 75 days on aluminum died, whereas cells p ... | 2008 | 18083857 |
| filters reduce the risk of bacterial transmission from contaminated heated humidifiers used with cpap for obstructive sleep apnea. | the treatment of choice for obstructive sleep apnea (osa) is nasal continuous positive airway pressure (ncpap) during sleep, but dryness of the upper airway compromises compliance. heated humidifiers may mitigate such noncompliance; however, recent observations suggest that their use, particularly if not cleaned, increases the risk of respiratory infections. humidifier water may be contaminated, but the long-held view that passive humidifiers cannot aerosolize water may obscure the perception of ... | 2007 | 18198803 |
| genotyping of human papillomaviruses by a novel one-step typing method with multiplex pcr and clinical applications. | we describe here a rapid, high-throughput genotyping procedure that allows the simultaneous detection of 16 high- and low-risk genital human papillomavirus (hpv) types by multiplex pcr in a single reaction tube. multiplex pcr is based on the amplification of hpv dna by sets of hpv genotype-specific primers, and the genotypes of hpv are visually identified by the sizes of amplicons after they are separated by capillary electrophoresis. the procedure does not include a hybridization step with hpv- ... | 2008 | 18234872 |
| chromate-tolerant bacteria for enhanced metal uptake by eichhornia crassipes (mart.). | a total of 85 chromate-resistant bacteria were isolated from the rhizosphere of water hyacinth grown in mariout lake, egypt, as well as the sediment and water of this habitat. only 4 (11%), 2 (8%), and 2 (8%) of isolates from each of the environments, respectively, were able to tolerate 200 mg cr (vi) l(-1). when these eight isolates were tested for their ability to tolerate other metals or to reduce chromate, they were shown to also be resistant to zn, mn, and pb, and to display different degre ... | 2007 | 18246718 |
| survey of bacterial diversity in chronic wounds using pyrosequencing, dgge, and full ribosome shotgun sequencing. | chronic wound pathogenic biofilms are host-pathogen environments that colonize and exist as a cohabitation of many bacterial species. these bacterial populations cooperate to promote their own survival and the chronic nature of the infection. few studies have performed extensive surveys of the bacterial populations that occur within different types of chronic wound biofilms. the use of 3 separate16s-based molecular amplifications followed by pyrosequencing, shotgun sanger sequencing, and denatur ... | 2008 | 18325110 |
| the airborne metagenome in an indoor urban environment. | the indoor atmosphere is an ecological unit that impacts on public health. to investigate the composition of organisms in this space, we applied culture-independent approaches to microbes harvested from the air of two densely populated urban buildings, from which we analyzed 80 megabases genomic dna sequence and 6000 16s rdna clones. the air microbiota is primarily bacteria, including potential opportunistic pathogens commonly isolated from human-inhabited environments such as hospitals, but non ... | 2008 | 18382653 |
| synthesis and qsar modeling of 2-acetyl-2-ethoxycarbonyl-1-[4(4'-arylazo)-phenyl]-n,n-dimethylaminophenyl aziridines as potential antibacterial agents. | the present communication deals with the synthesis of a series of 2-acetyl-2-ethoxycarbonyl-1-[4(4'-arylazo)-phenyl]-n,n-dimethylaminophenyl aziridines. the compounds were synthesized in excellent yields (70-80%) and the structures were established on the basis of consistent ir, (1)h nmr and elemental analysis data. the purity has been ascertained by chromatographic resolution using acetic acid--toluene (6:4v/v) as binary eluent. all the compounds have been tested for their antimicrobial activit ... | 2009 | 18396359 |
| analysis of bacterial dna in synovial tissue of tunisian patients with reactive and undifferentiated arthritis by broad-range pcr, cloning and sequencing. | bacteria and/or their antigens have been implicated in the pathogenesis of reactive arthritis (rea). several studies have reported the presence of bacterial antigens and nucleic acids of bacteria other than those specified by diagnostic criteria for rea in joint specimens from patients with rea and various arthritides. the present study was conducted to detect any bacterial dna and identify bacterial species that are present in the synovial tissue of tunisian patients with reactive arthritis and ... | 2008 | 18412942 |
| reverse reaction of malic enzyme for hco3- fixation into pyruvic acid to synthesize l-malic acid with enzymatic coenzyme regeneration. | malic enzyme [l-malate: nad(p)(+) oxidoreductase (ec 1.1.1.39)] catalyzes the oxidative decarboxylation of l-malic acid to produce pyruvic acid using the oxidized form of nad(p) (nad(p)(+)). we used a reverse reaction of the malic enzyme of pseudomonas diminuta ifo 13182 for hco(3)(-) fixation into pyruvic acid to produce l-malic acid with coenzyme (nadh) generation. glucose-6-phosphate dehydrogenase (ec1.1.1.49) of leuconostoc mesenteroides was suitable for coenzyme regeneration. optimum condit ... | 2008 | 18460807 |
| detoxification of organophosphate pesticides using an immobilized phosphotriesterase from pseudomonas diminuta. | a purified phosphotriesterase was successfully immobilized onto trityl agarose in a fixed bed reactor. a total of up to 9200 units of enzyme activity was immobilized onto 2.0 ml of trityl agarose (65 micromol trityl groups/ml agarose), where one unit is the amount of enzyme required to catalyze the hydrolysis of one micromole of paraoxon in one min. the immobilized enzyme was shown to behave chemically and kinetically similar to the free enzyme when paraoxon was utilized as a substrate. several ... | 1991 | 18597346 |
| structure of diethyl phosphate bound to the binuclear metal center of phosphotriesterase. | the bacterial phosphotriesterase (pte) from pseudomonas diminuta catalyzes the hydrolysis of organophosphate esters at rates close to the diffusion limit. x-ray diffraction studies have shown that a binuclear metal center is positioned in the active site of pte and that this complex is responsible for the activation of the nucleophilic water from solvent. in this paper, the three-dimensional structure of pte was determined in the presence of the hydrolysis product, diethyl phosphate (dep), and a ... | 2008 | 18702530 |
| chemotaxonomy of bacteria by comprehensive gc and gc-ms in electron impact and chemical ionisation mode. | the analysis of the cellular lipidic fraction of bacteria is described. after hydrolysis and methylation, the fatty acid methyl esters (fames) are determined by 1-d gc using the sherlock midi bacteria identification system, by comprehensive gc (gc x gc) and by gc-ms in electron impact (ei) and positive chemical ionisation (pci) mode. with gc x gc, the enhanced selectivity and group type separation provides a more complete elucidation of the fatty acids in microorganisms. gc-ei-ms and gc-pci-ms w ... | 2008 | 18792008 |
| influence of size, shape, and flexibility on bacterial passage through micropore membrane filters. | sterilization of fluids by means of microfiltration is commonly applied in research laboratories as well as in pharmaceutical and industrial processes. sterile micropore filters are subject to microbiological validation, where brevundimonas diminuta is used as a standard test organism. however, several recent reports on the ubiquitous presence of filterable bacteria in aquatic environments have cast doubt on the accuracy and validity of the standard filter-testing method. six different bacterial ... | 2008 | 18800559 |
| substrate-promoted formation of a catalytically competent binuclear center and regulation of reactivity in a glycerophosphodiesterase from enterobacter aerogenes. | the glycerophosphodiesterase (gpdq) from enterobacter aerogenes is a promiscuous binuclear metallohydrolase that catalyzes the hydrolysis of mono-, di-, and triester substrates, including some organophosphate pesticides and products of the degradation of nerve agents. gpdq has attracted recent attention as a promising enzymatic bioremediator. here, we have investigated the catalytic mechanism of this versatile enzyme using a range of techniques. an improved crystal structure (1.9 a resolution) i ... | 2008 | 18831553 |
| [degradation of hydrocarbons and their derivatives by a microbial association based on canadian pondweed]. | the degrading action of an aquatic plant-microbial association on the base of canadian pondweed (elodea canadensis) and its components (sterilized plant and two periphytonic strains, pseudomonas fluorescens e1-2.1 and brevundimonas diminuta e1-3.1) on crude oil, the water-soluble crude oil fraction, and individual test compounds (phenol, toluene, benzene, decalin, and naphthalene) was studied. it was found that the native association had a wider range and higher degree of degrading activity than ... | 2008 | 18924409 |
| first isolation of two colistin-resistant emerging pathogens, brevundimonas diminuta and ochrobactrum anthropi, in a woman with cystic fibrosis: a case report. | cystic fibrosis afflicted lungs support the growth of many bacteria rarely implicated in other cases of human infections. | 2008 | 19061488 |
| production of l-malic acid with fixation of hco3(-) by malic enzyme-catalyzed reaction based on regeneration of coenzyme on electrode modified by layer-by-layer self-assembly method. | malic enzyme prepared and purified from brevundimonas diminuta ifo13182 catalyzed the decarboxylation reaction of malate to pyruvate and co2 using nad+ as the coenzyme, and the reverse reaction was used in the present study for l-malic acid production with fixation of hco3(-) as a model compound for carbon source. the l-malic acid production was based on electrochemical regeneration of nadh on a carbon plate electrode modified by layer-by-layer adsorption of polymer-bound mediator (alginic acid ... | 2009 | 19147103 |
| functional annotation and three-dimensional structure of dr0930 from deinococcus radiodurans, a close relative of phosphotriesterase in the amidohydrolase superfamily. | dr0930, a member of the amidohydrolase superfamily in deinococcus radiodurans, was cloned, expressed, and purified to homogeneity. the enzyme crystallized in the space group p3121, and the structure was determined to a resolution of 2.1 a. the protein folds as a (beta/alpha)7beta-barrel, and a binuclear metal center is found at the c-terminal end of the beta-barrel. the purified protein contains a mixture of zinc and iron and is intensely purple at high concentrations. the purple color was deter ... | 2009 | 19159332 |
| structural determinants of the high thermal stability of ssopox from the hyperthermophilic archaeon sulfolobus solfataricus. | organophosphates (ops) constitute the largest class of insecticides used worldwide and certain of them are potent nerve agents. consequently, enzymes degrading ops are of paramount interest, as they could be used as bioscavengers and biodecontaminants. looking for a stable ops catalyst, able to support industrial process constraints, a hyperthermophilic phosphotriesterase (pte) (ssopox) was isolated from the archaeon sulfolobus solfataricus and was found to be highly thermostable. the solved 3d ... | 2009 | 19247785 |
| functional identification of incorrectly annotated prolidases from the amidohydrolase superfamily of enzymes. | the substrate profiles for two proteins from caulobacter crescentus cb15 (cc2672 and cc3125) and one protein (sgx9359b) derived from a dna sequence ( gi|44368820 ) isolated from the sargasso sea were determined using combinatorial libraries of dipeptides and n-acyl derivatives of amino acids. these proteins are members of the amidohydrolase superfamily and are currently misannotated in ncbi as catalyzing the hydrolysis of l-xaa-l-pro dipeptides. cc2672 was shown to catalyze the hydrolysis of l-x ... | 2009 | 19281183 |
| a probable aculeacin a acylase from the ralstonia solanacearum gmi1000 is n-acyl-homoserine lactone acylase with quorum-quenching activity. | the infection and virulence functions of diverse plant and animal pathogens that possess quorum sensing systems are regulated by n-acylhomoserine lactones (ahls) acting as signal molecules. ahl-acylase is a quorum quenching enzyme and degrades ahls by removing the fatty acid side chain from the homoserine lactone ring of ahls. this blocks ahl accumulation and pathogenic phenotypes in quorum sensing bacteria. | 2009 | 19426552 |
| evolution in the amidohydrolase superfamily: substrate-assisted gain of function in the e183k mutant of a phosphotriesterase-like metal-carboxylesterase. | the recent specialization for utilization of pesticides reported for pseudomonas diminuta phosphotriesterase (ppte) strongly suggests that this activity evolved from an enzyme endowed with promiscuous phosphotriesterase activity. such a putative "generalist" enzyme was recently proposed to be a member of the new phoshotriesterase-like lactonase family (pll). the promiscuous carboxylesterase and phosphodiesterase activities detected in ppte and plls in turn paved the way for the prediction of the ... | 2009 | 19438255 |
| modelling substrate specificity and enantioselectivity for lipases and esterases by substrate-imprinted docking. | previously, ways to adapt docking programs that were developed for modelling inhibitor-receptor interaction have been explored. two main issues were discussed. first, when trying to model catalysis a reaction intermediate of the substrate is expected to provide more valid information than the ground state of the substrate. second, the incorporation of protein flexibility is essential for reliable predictions. | 2009 | 19493341 |
| structure-based rational design of a phosphotriesterase. | in silico substrate docking of both stereoisomers of the pesticide chlorfenvinphos (cvp) in the phosphotriesterase from agrobacterium radiobacter identified two residues (f131 and w132) that prevent productive substrate binding and cause stereospecificity. a variant (w131h/f132a) was designed that exhibited ca. 480-fold and 8-fold increases in the rate of z-cvp and e-cvp hydrolysis, respectively, eliminating stereospecificity. | 2009 | 19502439 |
| update on biochemical properties of recombinant pseudomonas diminuta phosphotriesterase. | phosphotriesterase from pseudomonas diminuta (pte; ec 3.1.8.1) hydrolyzes organophosphate insecticides and chemical warfare agents. the two zinc cations in the active center can be substituted. co(2+)-containing pte is the most efficient but least stable isoform. gel filtration showed that pte is monomeric at the submicromolar concentrations used in kinetic assays. the analysis of the recombinant enzyme by x-ray fluorescence spectrometry and cct-icp-ms, confirms that recombinant zn-pte contains ... | 2009 | 19548794 |
| structure-based and random mutagenesis approaches increase the organophosphate-degrading activity of a phosphotriesterase homologue from deinococcus radiodurans. | an enzyme from the amidohydrolase family from deinococcus radiodurans (dr-oph) with homology to phosphotriesterase has been shown to exhibit activity against both organophosphate (op) and lactone compounds. we have characterized the physical properties of dr-oph and have found it to be a highly thermostable enzyme, remaining active after 3 h of incubation at 60 degrees c and withstanding incubation at temperatures up to 70 degrees c. in addition, it can withstand concentrations of at least 200 m ... | 2009 | 19631223 |
| nitrate removal using brevundimonas diminuta mtcc 8486 from ground water. | brevundimonas diminuta mtcc 8486, isolated from marine soil of coastal area of trivandrum, kerala, was used for biological removal of nitrate from ground water collected from kar village of pali district, rajasthan. the organism was found to be resistance for nitrate up to 10,000 mg l(-1). the optimum growth conditions for biological removal of nitrate were established in batch culture. the effect of carbon sources on nitrate removal was investigated using mineral salt medium (msm) containing 50 ... | 2009 | 19633395 |
| brevundimonas naejangsanensis sp. nov., a proteolytic bacterium isolated from soil, and reclassification of mycoplana bullata into the genus brevundimonas as brevundimonas bullata comb. nov. | a gram-negative, motile and rod-shaped bacterial strain, bio-tas2-2(t), of the class alphaproteobacteria, was isolated from a soil in korea and studied using a polyphasic taxonomic approach. strain bio-tas2-2(t) grew optimally at ph 7.5-8.5 and 30 degrees c and in the presence of 0-1.0 % (w/v) nacl. a neighbour-joining phylogenetic tree based on 16s rrna gene sequences showed that strain bio-tas2-2(t) fell within the clade comprising species of the genus brevundimonas, forming a coherent cluster ... | 2009 | 19643873 |
| molecular and kinetic characterization of mixed cultures degrading natural and synthetic estrogens. | the biodegradation of estradiol (e2), estrone (e1), and ethinylestradiol (ee2) was investigated using mixed bacterial cultures enriched from activated sludge. enrichments were carried out on e2 or ee2 in batch conditions with acetonitrile as additional carbon source. degradation experiments were performed both using hormones as sole carbon source or with an additional source. the hormones were completely degraded by these cultures. estradiol was rapidly converted to e1 within 24 h. thereafter, e ... | 2010 | 19685239 |
| magnetic circular dichroism study of a dicobalt(ii) complex with mixed 5- and 6-coordination: a spectroscopic model for dicobalt(ii) hydrolases. | the magnetic circular dichroism (mcd) study of [co(2)(mu-oh)(mu-ph(4)dba)(tmeda)(2)(otf)], in which ph(4)dba is the dinucleating bis(carboxylate) ligand dibenzofuran-4,6-bis(diphenylacetate) and tmeda is n,n,n',n'-tetramethylethylenediamine, is presented. this complex serves as an excellent spectroscopic model for a number of dicobalt(ii) enzymes and proteins that have both the mu-hydroxo, mu-carboxylato bridging and asymmetric 6- and 5-coordination. the low-temperature mcd spectrum of the model ... | 2009 | 19691327 |
| organophosphate hydrolase in brevundimonas diminuta is targeted to the periplasmic face of the inner membrane by the twin arginine translocation pathway. | a twin arginine translocation (tat) motif, involved in transport of folded proteins across the inner membrane, was identified in the signal peptide of the membrane-associated organophosphate hydrolase (oph) of brevundimonas diminuta. expression of the precursor form of oph carrying a c-terminal his tag in an opd-negative background and subsequent immunoblotting with anti-his antibodies showed that only the mature form of oph associated with the membrane and that the precursor form of oph was ent ... | 2009 | 19700527 |
| pseudomonads from rabbits and their sensitivity to antibiotics and natural antimicrobials. | the sensitivity/resistance of pseudomonas spp. isolated from rabbits gastrointestinal tract and faeces to antibiotics, enterocins and herbal extracts was tested in this study. the counts of pseudomonas-like bacteria were higher in faeces (3.23-6.16 log(10) cfu/ml/g) than in caecum (1.36-4.08 log(10) cfu/ml/g). nineteen isolates (16 faecal, 3 caecal) were oxidase positive. the strains were allotted by phenotypization to pseudomonas spp., brevundimonas diminuta and brevundimonasvesicularis. high p ... | 2010 | 19716146 |
| biodegradation of the herbicide trifluralin by bacteria isolated from soil. | trifluralin (alpha,alpha,alpha-trifluoro-2,6-dinitro-n,n-dipropyl-p-toluidine; tfl) is a pre-emergence, soil-incorporated herbicide that has been in agricultural use since the early 1960s and is moderately persistent in soil. the purpose of this study was to isolate and characterise tfl-resistant bacteria from a soil in which this pesticide has been used for the last four decades and to determine their ability to degrade tfl using hplc. eight bacteria were isolated by repeated subculture in liqu ... | 2003 | 19719679 |
| comparisons of subgingival microbial profiles of refractory periodontitis, severe periodontitis, and periodontal health using the human oral microbe identification microarray. | this study compared the subgingival microbiota of subjects with refractory periodontitis (rp) to those in subjects with treatable periodontitis (grs = good responders) or periodontal health (ph) using the human oral microbe identification microarray (homim). | 2009 | 19722792 |
| brevundimonas vancanneytii sp. nov., isolated from blood of a patient with endocarditis. | a gram-negative, rod-shaped, non-spore-forming bacterial strain, designated lmg 2337(t), was isolated from the blood of a patient with endocarditis and characterized. the strain was affiliated with the alphaproteobacterial genus brevundimonas, with brevundimonas diminuta lmg 2089(t) (98.3 % 16s rrna gene sequence similarity) and brevundimonas terrae ksl-145(t) (97.5 %) as its closest relatives. this affiliation was supported by chemotaxonomic data: the g+c content was 66.3 mol %, the major polar ... | 2010 | 19880635 |
| cotranslocation of methyl parathion hydrolase to the periplasm and of organophosphorus hydrolase to the cell surface of escherichia coli by the tat pathway and ice nucleation protein display system. | a genetically engineered escherichia coli strain coexpressing organophosphorus hydrolase (oph) and methyl parathion hydrolase (mph) was constructed for the first time by cotransforming two compatible plasmids. since these two enzymes have different substrate specificities, the coexpression strain showed a broader substrate range than strains expressing either one of the hydrolases. to reduce the mass transport limitation of organophosphates (ops) across the cell membrane, mph and oph were simult ... | 2010 | 19933341 |
| cotranslocation of methyl parathion hydrolase to the periplasm and of organophosphorus hydrolase to the cell surface of escherichia coli by the tat pathway and ice nucleation protein display system. | a genetically engineered escherichia coli strain coexpressing organophosphorus hydrolase (oph) and methyl parathion hydrolase (mph) was constructed for the first time by cotransforming two compatible plasmids. since these two enzymes have different substrate specificities, the coexpression strain showed a broader substrate range than strains expressing either one of the hydrolases. to reduce the mass transport limitation of organophosphates (ops) across the cell membrane, mph and oph were simult ... | 2010 | 19933341 |
| use of hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters. | filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. using hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. we show that con ... | 2010 | 19966023 |
| use of hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters. | filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. using hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. we show that con ... | 2010 | 19966023 |
| sinorhizobium meliloti phospholipase c required for lipid remodeling during phosphorus limitation. | rhizobia are gram-negative soil bacteria able to establish nitrogen-fixing root nodules with their respective legume host plants. besides phosphatidylglycerol, cardiolipin, and phosphatidylethanolamine, rhizobial membranes contain phosphatidylcholine (pc) as a major membrane lipid. under phosphate-limiting conditions of growth, some bacteria replace their membrane phospholipids with lipids lacking phosphorus. in sinorhizobium meliloti, these phosphorus-free lipids are sulfoquinovosyl diacylglyce ... | 2010 | 20018679 |
| sinorhizobium meliloti phospholipase c required for lipid remodeling during phosphorus limitation. | rhizobia are gram-negative soil bacteria able to establish nitrogen-fixing root nodules with their respective legume host plants. besides phosphatidylglycerol, cardiolipin, and phosphatidylethanolamine, rhizobial membranes contain phosphatidylcholine (pc) as a major membrane lipid. under phosphate-limiting conditions of growth, some bacteria replace their membrane phospholipids with lipids lacking phosphorus. in sinorhizobium meliloti, these phosphorus-free lipids are sulfoquinovosyl diacylglyce ... | 2010 | 20018679 |
| high-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories. | matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms) is suitable for high-throughput and rapid diagnostics at low costs and can be considered an alternative for conventional biochemical and molecular identification systems in a conventional microbiological laboratory. first, we evaluated maldi-tof ms using 327 clinical isolates previously cultured from patient materials and identified by conventional techniques (vitek-ii, api, and biochemical tests). discre ... | 2010 | 20053859 |
| an automated system for rapid non-destructive enumeration of growing microbes. | the power and simplicity of visual colony counting have made it the mainstay of microbiological analysis for more than 130 years. a disadvantage of the method is the long time required to generate visible colonies from cells in a sample. new rapid testing technologies generally have failed to maintain one or more of the major advantages of culture-based methods. | 2010 | 20062794 |
| selective enumeration strategies for brevundimonas diminuta from drinking water. | brevundimonas diminuta is used as a control organism for validating the efficiency of water filtration systems. since these protocols use nonselective growth media, heterotrophic plate count bacteria (hpcs) indigenous to the water distribution system may interfere with b. diminuta enumeration, thus leading to inaccurate assessment of the filter's microbial reduction capability. this could negatively impact public health as unsafe drinking water may be produced. this study was conducted to evalua ... | 2010 | 20087629 |
| bacterial symbionts of the brown planthopper, nilaparvata lugens (homoptera: delphacidae). | the brown planthopper (nilaparvata lugens stål), the most destructive pest of rice, has been identified, including biotypes with high virulence towards previously resistant rice varieties. there have also been many reports of a yeast-like symbiont of n. lugens, but little is known about the bacterial microbes. in this study, we examined the bacterial microbes in n. lugens and identified a total of 18 operational taxonomic units (otus) representing four phyla (proteobacteria, firmicutes, actinoba ... | 2010 | 20097822 |
| lactonases with organophosphatase activity: structural and evolutionary perspectives. | serum paraoxonase (pon1) is well recognized for its ability to hydrolyze arylesters, toxic oxon metabolites of organophosphate insecticides and nerve agents. pon1 is a member of gene family including also pon2 and pon3; however, the later two enzymes have very limited arylesterase and practically no organophosphatase activity. we have established that all three pons are lactonases/lactonyzing enzymes with overlapping, but also distinct substrate specificity. dihydrocoumarin (dhc), long chain fat ... | 2010 | 20122908 |
| a localized multimeric anchor attaches the caulobacter holdfast to the cell pole. | caulobacter crescentus attachment is mediated by the holdfast, a complex of polysaccharide anchored to the cell by hfaa, hfab and hfad. we show that all three proteins are surface exposed outer membrane (om) proteins. hfaa is similar to fimbrial proteins and assembles into a high molecular weight (hmw) form requiring hfad, but not holdfast polysaccharide. the hfad hmw form is dependent on hfaa but not on holdfast polysaccharide. we show that hfaa and hfad form homomultimers and that they require ... | 2010 | 20233308 |
| the analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 gs flx titanium pyrosequencer. | although mice have long served as an animal model for periodontitis, information on the composition of their indigenous oral microbiota is limited. the aim of the current study was to characterize mouse oral bacterial flora by applying extensive parallel pyrosequencing using the latest model pyrosequencer, a roche/454 genome sequencer flx titanium. in addition, the effect of toll-like receptor (tlr) 2 deficiency on oral microbiota was evaluated. | 2010 | 20370919 |
| recurrent isolation of extremotolerant bacteria from the clean room where phoenix spacecraft components were assembled. | the microbial burden of the phoenix spacecraft assembly environment was assessed in a systematic manner via several cultivation-based techniques and a suite of nasa-certified, cultivation-independent biomolecule-based detection assays. extremotolerant bacteria that could potentially survive conditions experienced en route to mars or on the planet's surface were isolated with a series of cultivation-based assays that promoted the growth of a variety of organisms, including spore formers, mesophil ... | 2010 | 20446872 |
| mutation of outer-shell residues modulates metal ion co-ordination strength in a metalloenzyme. | the metal ion co-ordination sites of many metalloproteins have been characterized by a variety of spectroscopic techniques and small-molecule model systems, revealing many important insights into the structural determinants of metal ion co-ordination. however, our understanding of this fundamentally and practically important phenomenon remains frustratingly simplistic; in many proteins it is essentially impossible to predict metal ion specificity and the effects of remote 'outer-shell' residues ... | 2010 | 20459397 |
| quantitative real-time pcr and fluorescence in situ hybridization approaches for enumerating brevundimonas diminuta in drinking water. | brevundimonas diminuta is a small gram-negative bacterium used for validation of membranes and filters used in the pharmaceutical and drinking water treatment industries. current assays are time consuming, nonselective, and may be subject to interference by competing indigenous microorganisms. the focus of this study is to develop rapid and specific enumeration methodologies for b. diminuta. quantitative real-time polymerase chain reaction (qpcr) and fluorescence in situ hybridization (fish) ass ... | 2010 | 20495940 |
| a bacterial extracellular dna inhibits settling of motile progeny cells within a biofilm. | summary in natural systems, bacteria form complex, surface-attached communities known as biofilms. this lifestyle presents numerous advantages compared with unattached or planktonic life, such as exchange of nutrients, protection from environmental stresses and increased tolerance to biocides. despite such benefits, dispersal also plays an important role in escaping deteriorating environments and in successfully colonizing favourable, unoccupied habitat patches. the alpha-proteobacterium cauloba ... | 2010 | 20598083 |
| pharmacokinetics of opda, an organophosphorus hydrolase, in the african green monkey. | organophosphorus (op) pesticides are a broad class of acetylcholinesterase inhibitors that are responsible for tremendous morbidity and mortality worldwide, contributing to an estimated 300,000 deaths annually. current pharmacotherapy for acute op poisoning includes the use of atropine, an oxime, and benzodiazepines. however, even with such therapy, the mortality from these agents are as high as 40%. enzymatic hydrolysis of ops is an attractive new potential therapy for acute op poisoning. a num ... | 2010 | 20599794 |
| improving the promiscuous nerve agent hydrolase activity of a thermostable archaeal lactonase. | the thermostable phosphotriesterase-like lactonase from sulfolobus solfataricus (ssopox) hydrolyzes lactones and, at a lower rate, neurotoxic organophosphorus compounds. the persistent demand of detoxification tools in the field of agricultural wastes and restoring of conditions after terrorist acts prompted us to exploit ssopox as a "starter" to evolve its ancillary nerve agents hydrolytic capability. a directed evolution strategy yielded, among several variants, the single mutant w263f with k( ... | 2010 | 20667718 |
| rapid detection of pseudomonas aeruginosa from positive blood cultures by quantitative pcr. | pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. the present study was aimed to develop a novel quantitative pcr (qpcr) assay, using ecfx as the specific target gene, for the rapid and accurate identification of p. aeruginosa from positive blood cultures (bcs). | 2010 | 20684778 |
| obstacles of multiplex real-time pcr for bacterial 16s rdna: primer specifity and dna decontamination of taq polymerase. | background: the detection of a broad range of bacteria by pcr is applied for the screening of blood and blood products with special attention to platelet concentrates. for practical use it is desirable that detection systems include gram-positive, gram-negative and non-gram-stainable bacteria. it is quite challenging to achieve high sensitivity along with a clear negative control with pcr reagents, because especially taq polymerase is contaminated with traces of bacterial dna. methods: bacterial ... | 2010 | 20737013 |
| absence of high-risk human papillomavirus (hpv) detection in endocervical adenocarcinoma with gastric morphology and phenotype. | a subset of endocervical-type mucinous adenocarcinomas (acs) of the uterine cervix exhibit a gastric phenotype and morphology, as reported in cases of minimal deviation ac in which the presence of human papillomavirus (hpv) has been rarely detected. to investigate the hpv-independent pathway of carcinogenesis in cases of gastric-type ac, we investigated the common high-risk hpv (hr-hpv) status in 52 nonsquamous cell carcinomas, using a pcr-based typing method and immunohistochemistry of p16ink4a ... | 2010 | 20829441 |
| evaluation of an innovative antimicrobial surgical glove technology to reduce the risk of microbial passage following intraoperative perforation. | surgical gloves provide a protective barrier for patients and members of the surgical team. although glove integrity is important in an era of blood-borne pathogens, little data exist on bacterial passage after glove perforation. this study evaluated the impact of antimicrobial surgical gloves in reducing microbial passage after glove puncture in a model of wound contamination. | 2011 | 20864217 |
| isothermal dna amplification using the t4 replisome: circular nicking endonuclease-dependent amplification and primase-based whole-genome amplification. | in vitro reconstitution of the bacteriophage t4 replication machinery provides a novel system for fast and processive isothermal dna amplification. we have characterized this system in two formats: (i) in circular nicking endonuclease-dependent amplification (cnda), the t4 replisome is supplemented with a nicking endonuclease (nb.bbvci) and a reverse primer to generate a well-defined uniform double-stranded linear product and to achieve up to 1100-fold linear amplification of a plasmid in 1 h. ( ... | 2010 | 20921065 |
| biodegradation of a biocide (cu-n-cyclohexyldiazenium dioxide) component of a wood preservative by a defined soil bacterial community. | the wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. one of these is cu-hdo, containing the chelation product of copper and n-cyclohexyldiazenium dioxide (hdo). in this study, the fate of isotope-labeled ((13)c) and nonlabeled ((12)c) cu-hdo incorporated in wood sawdust mixed with soil was investigated. hdo concentration was monitored by high-pressure liq ... | 2010 | 20952650 |
| phosphotriesterase variants with high methylphosphonatase activity and strong negative trade-off against phosphotriesters. | the most lethal organophosphorus nerve agents (na), like sarin, soman, agent-vx and russian-vx, share a methylphosphonate moiety. pseudomonas diminuta phosphotriesterase (pte) catalyses the hydrolysis of methylphosphonate na analogues with a catalytic efficiency orders of magnitude lower than that towards the pesticide paraoxon. with a view to obtaining pte variants that more readily accept methylphosphonate na, ~75,000 pte variants of the substrate-binding residues gly-60, ile-106, leu-303 and ... | 2010 | 21037279 |
| characterisation of source-separated household waste intended for composting. | large-scale composting of source-separated household waste has expanded in recent years in the nordic countries. one problem can be low ph at the start of the process. incoming biowaste at four composting plants was characterised chemically, physically and microbiologically. the ph of food waste ranged from 4.7 to 6.1 and organic acid concentration from 24 to 81 mmol kg(-1). the bacterial diversity in the waste samples was high, with all samples dominated by gammaproteobacteria, particularly pse ... | 2010 | 21075618 |
| characterization of a phosphotriesterase-like lactonase from sulfolobus solfataricus and its immobilization for disruption of quorum sensing. | ssopox, a bifunctional enzyme with organophosphate hydrolase and n-acyl homoserine lactonase activities from the hyperthermophilic archaeon sulfolobus solfataricus, was overexpressed and purified from recombinant pseudomonas putida kt2440 with a yield of 9.4 mg of protein per liter of culture. the enzyme has a preference for n-acyl homoserine lactones (ahls) with acyl chain lengths of at least 8 carbon atoms, mainly due to lower k(m) values for these substrates. the highest specificity constant ... | 2010 | 21183649 |
| characterization of a phosphotriesterase-like lactonase from sulfolobus solfataricus and its immobilization for disruption of quorum sensing. | ssopox, a bifunctional enzyme with organophosphate hydrolase and n-acyl homoserine lactonase activities from the hyperthermophilic archaeon sulfolobus solfataricus, was overexpressed and purified from recombinant pseudomonas putida kt2440 with a yield of 9.4 mg of protein per liter of culture. the enzyme has a preference for n-acyl homoserine lactones (ahls) with acyl chain lengths of at least 8 carbon atoms, mainly due to lower k(m) values for these substrates. the highest specificity constant ... | 2010 | 21183649 |
| catalytic mechanism and three-dimensional structure of adenine deaminase. | adenine deaminase (ade) catalyzes the conversion of adenine to hypoxanthine and ammonia. the enzyme isolated from escherichia coli using standard expression conditions was low for the deamination of adenine (k(cat) = 2.0 s(-1); k(cat)/k(m) = 2.5 × 10(3) m(-1) s(-1)). however, when iron was sequestered with a metal chelator and the growth medium was supplemented with mn(2+) prior to induction, the purified enzyme was substantially more active for the deamination of adenine with k(cat) and k(cat)/ ... | 2011 | 21247091 |
| prevalence and significance of fluoroquinolone resistant escherichia coli in patients undergoing transrectal ultrasound guided prostate needle biopsy. | we estimated the prevalence of fluoroquinolone resistant escherichia coli in patients undergoing repeat transrectal ultrasound guided prostate needle biopsy and identified high risk groups. | 2011 | 21334021 |
| indigenous organophosphate-degrading (opd) plasmid pcms1 of brevundimonasdiminuta is self-transmissible and plays a key role in horizontal mobility of the opd gene. | a fosmid library of the 66kb indigenous organophosphate-degrading (opd) plasmid pcms1 of brevundimonas diminuta was tagged with mini-transposon eztn5 <r6k?ori/kan-2>, to determine its sequence using transposon-specific primers. the sequence revealed the presence of a number of tra genes suggesting their role in conjugal transfer of pcms1. consistent with the presence of the tra genes, the b. diminuta plasmid, pcms1::tet, generated by replacing the opd gene with opd::tet, served as a donor for tr ... | 2011 | 21354204 |
| crystallization and preliminary x-ray diffraction analysis of the hyperthermophilic sulfolobus islandicus lactonase. | phosphotriesterase-like lactonases (plls) constitute an interesting family of enzymes that are of paramount interest in biotechnology with respect to their catalytic functions. as natural lactonases, they may act against pathogens such as-ápseudomonas aeruginosa by shutting down their quorum-sensing system (quorum quenching) and thus decreasing pathogen virulence. owing to-átheir promiscuous phosphotriesterase activity, which can inactivate toxic organophosphorus compounds such as pesticides and ... | 2011 | 21393842 |
| identification of an n-terminal formylated, two-peptide bacteriocin from enterococcus faecalis 710c. | enterococcus faecalis 710c, isolated from beef product, has a broad antimicrobial activity spectrum against foodborne pathogens. two bacteriocins, enterocin 7a (ent7a) and enterocin 7b (ent7b), were purified from the culture supernatant of e. faecalis 710c and characterized using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and electrospray infusion tandem mass spectrometry analyses. these data and subsequent genetic analysis showed that ent7a and ent7b are produc ... | 2011 | 21469734 |
| first case of fulminant sepsis due to wohlfahrtiimonas chitiniclastica. | we report the first case of fulminant sepsis due to wohlfahrtiimonas chitiniclastica. this case is also the first one reported in south america. we emphasize the importance of recognizing bacteria that live in the larvae of a parasitic fly as the causative agent of severe infections in homeless patients. | 2011 | 21471333 |
| microbiology of animal bite wound infections. | the microbiology of animal bite wound infections in humans is often polymicrobial, with a broad mixture of aerobic and anaerobic microorganisms. bacteria recovered from infected bite wounds are most often reflective of the oral flora of the biting animal, which can also be influenced by the microbiome of their ingested prey and other foods. bacteria may also originate from the victim's own skin or the physical environment at the time of injury. our review has focused on bite wound infections in ... | 2011 | 21482724 |
| the importance of accurate microorganism identification in microbial challenge tests of membrane filters--part i. | microbial challenge testing is a common procedure to determine the retention efficiency, performance, and validity of a sterilizing-grade filter. the astm 838-05 standard describes a bacteria challenge test procedure based on brevundimonas diminuta (atcc 19146), routinely used to verify a 0.2 μm rated sterilizing-grade filter. process validation procedures most often also utilize b. diminuta (atcc 19146), but instead of the standard procedures and fluids, process, and product parameters are empl ... | 2011 | 21502070 |
| the n-terminal nucleophile serine of cephalosporin acylase executes the second autoproteolytic cleavage and acyl-peptide hydrolysis. | cephalosporin acylase precursor is translated as a single polypeptide chain and folds into a self-activating pre-protein. activation requires two peptide bond cleavages that excise an internal spacer to form the mature αβ heterodimer. using q-tof lc/ms, we located the second cleavage site between e159 and g160, and detected the corresponding 10 aa spacer g160dppdladqg169 of ca mutants. the site of the second cleavage depended on e159: moving e into the spacer or removing 5-10 residues from the s ... | 2011 | 21576250 |
| brevundimonas diminuta keratitis. | purpose:: to report the first documented case of keratitis secondary to the gram-negative bacillus brevundimonas diminuta. methods:: a 66-year-old woman with an ocular history of bilateral soft contact lens wear presented with a large, 6×9-mm, culture-positive pseudomonas aeruginosa-induced corneal ulcer. after weeks of therapy, she developed secondary keratitis. repeat cultures grew a rare, gram-negative bacillus, b. diminuta. results:: targeted therapy followed by adjustments based on repeat b ... | 2011 | 21617535 |
| crystallization and preliminary crystallographic analysis of the phosphotriesterase-like lactonase from geobacillus kaustophilus. | gk1506 from the thermophilic bacterium geobacillus kaustophilus is a member of the phosphotriesterase-like lactonases, which can catalyze the hydrolysis of a broad range of compounds with different chemical properties. it is of particular interest because of its high thermostability and its dual activity towards organophosphate compounds and some lactones. these properties make gk1506 an attractive target for future enzyme engineering and use in practical applications. in order to resolve the cr ... | 2011 | 21795796 |
| synthesis and exploration of qsar model of 2-methyl-3-[2-(2-methylprop-1-en-1-yl)-1h-benzimidazol-1-yl]pyrimido[1,2-a]benzimidazol-4(3h)-one as potential antibacterial agents. | present communication deals with the synthesis of novel 2-methyl-3-[2-(2-methylprop-1-en-1-yl)-1h-benzimidazol-1-yl]pyrimido[1,2-a]benzimidazol-4(3h)-one derivatives under phase transfer catalysis (ptc) conditions using benzyl triethyl ammonium chloride (bteac) as ptc. it also elicits the studies on in vitro antimicrobial evaluation of synthesized compounds against a representative genera of gram-negative and gram-positive bacteria i.e., bacillus subtilis, staphylococcus aureus, pseudomonas dimi ... | 2011 | 21699375 |
| genome sequences of eight morphologically diverse alphaproteobacteria. | the alphaproteobacteriacomprise morphologically diverse bacteria, including many species of stalked bacteria. here we announce the genome sequences of eight alphaproteobacteria, including the first genome sequences of species belonging to the genera asticcacaulis, hirschia, hyphomicrobium, and rhodomicrobium. | 2011 | 21705585 |
| differential radial capillary action of ligand assay for high-throughput detection of protein-metabolite interactions. | interactions of proteins with low-molecular-weight ligands, such as metabolites, cofactors, and allosteric regulators, are important determinants of metabolism, gene regulation, and cellular homeostasis. pharmaceuticals often target these interactions to interfere with regulatory pathways. we have developed a rapid, precise, and high-throughput method for quantitatively measuring protein-ligand interactions without the need to purify the protein when performed in cells with low background activi ... | 2011 | 21876132 |
| factors influencing the microbial composition of metalworking fluids and potential implications for machine operator's lung. | hypersensitivity pneumonitis, also known as "machine operator's lung" (mol), has been related to microorganisms growing in metalworking fluids (mwfs), especially mycobacterium immunogenum. we aimed to (i) describe the microbiological contamination of mwfs and (ii) look for chemical, physical, and environmental parameters associated with variations in microbiological profiles. we microbiologically analyzed 180 mwf samples from nonautomotive plants (e.g., screw-machining or metal-cutting plants) i ... | 2012 | 22057869 |