Publications

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biofilm-induced changes to the composite surface.composites may undergo biodegradation in the oral cavity. the objective was to investigate the effect of single- and multi-species biofilms on the surface roughness and topography of two composites.201728554609
effects of streptococcus sanguinis bacteriocin on deformation, adhesion ability, and young's modulus of candida albicans.in order to study the thallus changes on microscopic morphology and mechanical properties of candida albicans antagonized by streptococcus sanguinis bacteriocin, the adhesion ability and young's modulus of thalli and hypha of candida albicans were measured by the relative measurement method using atomic force microscope's (afm) tapping model. the results showed that the average adhesion ability and young's modulus of thalli were 7.35 ± 0.77 nn and 7.33 ± 1.29 mpa, respectively; the average adhes ...201728612025
characterization of non-dialyzable constituents from cranberry juice that inhibit adhesion, co-aggregation and biofilm formation by oral bacteria.an extract prepared from cranberry juice by dialysis known as nondialyzable material (ndm) has been shown previously to possess anti-adhesion activity toward microbial species including oral bacteria, uropathogenic escherichia coli and helicobacter pylori. bioassay-guided fractionation of cranberry ndm was therefore undertaken to identify the anti-adhesive constituents. an aqueous acetone-soluble fraction (ndmac) obtained from sephadex lh-20 inhibited adhesion-linked activities by oral bacteria, ...201728470309
microbiomes of site-specific dental plaques from children with different caries status.the oral microbiota associated with the initiation and progression of dental caries has yet to be fully characterized. the human oral microbe identification using next-generation sequencing (homings) approach was used to analyze the microbiomes of site-specific supragingival dental plaques from children with different caries status. fifty-five children (2 to 7 years of age) were assessed at baseline and at 12 months and grouped as caries free (cf), caries active with enamel lesions (cae), and ca ...201728507066
bacterial adherence to graft tissues in static and flow conditions.various conduits and stent-mounted valves are used as pulmonary valve graft tissues for right ventricular outflow tract reconstruction with good hemodynamic results. valve replacement carries an increased risk of infective endocarditis (ie). recent observations have increased awareness of the risk of ie after transcatheter implantation of a stent-mounted bovine jugular vein valve. this study focused on the susceptibility of graft tissue surfaces to bacterial adherence as a potential risk factor ...201728712577
the influence of surface texture and wettability on initial bacterial adhesion on titanium and zirconium oxide dental implants.this study aims to investigate bacterial adhesion on different titanium and ceramic implant surfaces, to correlate these findings with surface roughness and surface hydrophobicity, and to define the predominant factor for bacterial adhesion for each material.201728714053
impact of a 7-day field training on oral health condition in japan ground self-defense force personnel.in the japan ground self-defense force (jgsdf), personnel periodically perform intensive training that mimics the conditions seen in battle and during natural disasters. military training involves intensive, stressful conditions, and changes in immune responses have been found in personnel following training. good oral condition is important for military personnel to fulfill their duties; however, they have difficulty performing daily oral care under training conditions. in this study, we invest ...201728810984
anti-caries effects of dental adhesives containing quaternary ammonium methacrylates with different chain lengths.the objectives of this study were to investigate the effects of dental adhesives containing quaternary ammonium methacrylates (qams) with different alkyl chain lengths (cl) on ecological caries prevention in vitro. five qams were synthesized with a cl = 3, 6, 9, 12, and 16 and incorporated into adhesives. micro-tensile bond strength and surface charge density were used to measure the physical properties of the adhesives. the proportion change in three-species biofilms consisting of streptococcus ...201728773004
novel dental adhesive with biofilm-regulating and remineralization capabilities.the mechanical properties and anti-caries effect of a novel anti-caries adhesive containing poly (amidoamine) dendrimer (pamam) and dimethylaminododecyl methacrylate (dmaddm) were investigated for the first time. microtensile bond strength and surface charge density were measured for the novel anti-caries adhesives. streptococcus mutans, streptococcus sanguinis, and streptococcus gordonii were chosen to form three-species biofilms. lactic acid assay, mtt (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphen ...201728772391
microbiome associated with severe caries in canadian first nations children.young indigenous children in north america suffer from a higher degree of severe early childhood caries (s-ecc) than the general population, leading to speculation that the etiology and characteristics of the disease may be distinct in this population. to address this knowledge gap, we conducted the first microbiome analysis of an indigenous population using modern molecular techniques. we investigated the caries-associated microbiome among canadian first nations children with s-ecc. thirty firs ...201728709393
effect of arginine on the growth and biofilm formation of oral bacteria.alkali production via arginine deiminase system (ads) of oral bacteria plays a significant role in oral ecology, ph homeostasis and inhibition of dental caries. ads activity in dental plaque varies greatly between individuals, which may profoundly affect their susceptibility to caries.201728668766
distinct biological potential of streptococcus gordonii and streptococcus sanguinis revealed by comparative genome analysis.streptococcus gordonii and streptococcus sanguinis are pioneer colonizers of dental plaque and important agents of bacterial infective endocarditis (ie). to gain a greater understanding of these two closely related species, we performed comparative analyses on 14 new s. gordonii and 5 s. sanguinis strains using various bioinformatics approaches. we revealed s. gordonii and s. sanguinis harbor open pan-genomes and share generally high sequence homology and number of core genes including virulence ...201728592797
targeting of streptococcus mutans biofilms by a novel small molecule prevents dental caries and preserves the oral microbiome.dental caries is a costly and prevalent disease characterized by the demineralization of the tooth's enamel. disease outcome is influenced by host factors, dietary intake, cariogenic bacteria, and other microbes. the cariogenic bacterial species streptococcus mutans metabolizes sucrose to initiate biofilm formation on the tooth surface and consequently produces lactic acid to degrade the tooth's enamel. persistence of s. mutans biofilms in the oral cavity can lead to tooth decay. to date, no ant ...201728571487
alternative sweeteners influence the biomass of oral biofilm.compact-structured oral biofilm accumulates acids that upon prolonged exposure to tooth surface, causes demineralisation of enamel. this study aimed to assess the effect of alternative sweeteners equal stevia(®), tropicana slim(®), pal sweet(®) and xylitol on the matrix-forming activity of plaque biofilm at both the early and established stages of formation.201728448807
fluoride depletes acidogenic taxa in oral but not gut microbial communities in mice.fluoridation of drinking water and dental products prevents dental caries primarily by inhibiting energy harvest in oral cariogenic bacteria (such as streptococcus mutans and streptococcus sanguinis), thus leading to their depletion. however, the extent to which oral and gut microbial communities are affected by host fluoride exposure has been underexplored. in this study, we modeled human fluoride exposures to municipal water and dental products by treating mice with low or high levels of fluor ...201728808691
ecological effect of arginine on oral microbiota.dental caries is closely associated with the microbial dybiosis between acidogenic/aciduric pathogens and alkali-generating commensal bacteria colonized in the oral cavity. our recent studies have shown that arginine may represent a promising anti-caries agent by modulating microbial composition in an in vitro consortium. however, the effect of arginine on the oral microbiota has yet to be comprehensively delineated in either clinical cohort or in vitro biofilm models that better represent the m ...201728775282
comparative effect of photodynamic therapy on separated or mixed cultures of streptococcus mutans and streptococcus sanguinis.antimicrobial photodynamic therapy (apdt) has shown to exert a bactericidal effect against streptococcus sanguinis and streptococcus mutans. however, this efficacy has been reported for either type of bacteria separately. bacterial suspensions of both strains, separately or together, were treated with concentrations of methylene blue (mb) and rose bengal (rb). suspensions were irradiated with a light-emitting diode lamp (λ center at 625nm for mb and λ center at 515nm for rb) using a fluence of 1 ...201728546111
cilostazol inhibits the expression of hnrnp a2/b1 and cytokines in human dermal microvascular endothelial cells.hnrnp a2/b1 has been identified as a target antigen of anti-endothelial cell iga antibody in patients with behçet's disease (bd). in addition, increased expression of cellular hnrnp a2/b1 is stimulated by streptococcus sanguinis or the sera from patients with bd. we aimed to investigate the effects of cilostazol on the expression of hnrnp a2/b1 and chemokines in human dermal microvascular endothelial cells (hdmecs).201728850024
behçet disease serum is immunoreactive to neurofilament medium which share common epitopes to bacterial hsp-65, a putative trigger.autoimmune and dysimmune inflammatory mechanisms on a genetically susceptible background are implicated in the etiology of behçet's disease (bd). heat-shock protein-65 (hsp-65) derived from streptococcus sanguinis was proposed as a triggering factor based on its homology with human hsp-60. however, none of the autoantigens identified so far in sera from bd share common epitopes with bacterial hsp-65 or has a high prevalence. here, we report that sera from bd patients are immunoreactive against f ...201728844827
bacterial attachment on titanium surfaces is dependent on topography and chemical changes induced by nonthermal atmospheric pressure plasma.here, we investigated the antibacterial effects of chemical changes induced by nonthermal atmospheric pressure plasma (ntapp) on smooth and rough ti. the morphologies of smooth and rough surfaces of ti were examined using scanning electron microscopy (sem). both ti specimens were then treated for 10 min by ntapp with nitrogen gas. the surface roughness, chemistry, and wettability were examined by optical profilometry, x-ray photoelectron spectroscopy, and water contact angle analysis, respective ...201728746053
is spxa2 involved in hydrogen peroxide production and competence development in streptococcus sanguinis?the objective of the present study was to investigate whether streptococcus sanguinis spxa2 plays a role in competence development and endogenous h2o2 generation, and whether the spxa2 cys10-xx-cys13 (cxxc) motif is involved in competence development.201728693663
air-abrasive disinfection of implant surfaces in a simulated model of periimplantitis.this in vitro study aimed to evaluate the ability of air-powder abrasion to decontaminate dental implants.201728486355
a rare case of streptococcus sanguinis mycotic popliteal aneurysm.mycotic popliteal aneurysms are not a common phenomenon. they can initially be easily confused with other more trivial conditions such as a baker's cyst. we present a case of a patient presenting with a progressively worsening leg swelling which was initially misdiagnosed. only until symptoms rapidly progressed was a popliteal aneurysm diagnosed. to our knowledge this is the only identified case of a streptococcus sanguinis mycotic popliteal aneurysm.201428663804
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