| pseudomonas aeruginosa facilitates campylobacter jejuni growth in biofilms under oxic flow conditions. | we investigated the growth of campylobacter jejuni in biofilms with pseudomonas aeruginosa under oxic flow conditions. we observed the growth of c. jejuni in mono-culture, deposited on pre-established p. aeruginosa biofilms, and co-inoculated with p. aeruginosa. in mono-culture, c. jejuni was unable to form biofilms. however, deposited c. jejuni continuously grew on pre-established p. aeruginosa biofilms for a period of 3 days. the growth of scattered c. jejuni clusters was strictly limited to t ... | 2015 | 26610432 |
| seroprevalence in chickens against campylobacter jejuni flagellar capping protein (flid) in selected areas of the united states. | campylobacter jejuni is a causative pathogen of human acute bacterial gastroenteritis. infected poultry products are regarded as a major source for human c. jejuni infection. the flagellar capping protein (flid) is highly conserved among c. jejuni strains/isolates and is antigenic as analysed by immunoblot. in this study, we used the flid protein as a probe to survey the prevalence of c. jejuni antibodies in chickens from two areas in the united states. a total of 394 samples were tested. sera f ... | 2016 | 26603949 |
| conservation and covariance in small bacterial phosphoglycosyltransferases identify the functional catalytic core. | phosphoglycosyltransferases (pgts) catalyze the transfer of a c1'-phosphosugar from a soluble sugar nucleotide diphosphate to a polyprenol phosphate. these enzymes act at the membrane interface, forming the first membrane-associated intermediates in the biosynthesis of cell-surface glycans and glycoconjugates, including glycoproteins, glycolipids, and the peptidoglycan in bacteria. pgts vary greatly in both their membrane topologies and their substrate preferences. pgts, such as mray and weca, a ... | 2015 | 26600273 |
| a seventeen-year observation of the antimicrobial susceptibility of clinical campylobacter jejuni and the molecular mechanisms of erythromycin-resistant isolates in beijing, china. | to investigate the dynamic development of the antimicrobial resistance of campylobacter jejuni isolated from human diarrhea in beijing, china, between 1994 and 2010, and to further analyze the molecular mechanisms of erythromycin-resistant strains. | 2016 | 26594011 |
| diversity and relatedness of shiga toxin-producing escherichia coli and campylobacter jejuni between farms in a dairy catchment. | the aim of this study was to examine the population structure, transmission and spatial relationship between genotypes of shiga toxin-producing escherichia coli (stec) and campylobacter jejuni, on 20 dairy farms in a defined catchment. pooled faecal samples (n = 72) obtained from 288 calves were analysed by real-time polymerase chain reaction (rtpcr) for e. coli serotypes o26, o103, o111, o145 and o157. the number of samples positive for e. coli o26 (30/72) was high compared to e. coli o103 (7/7 ... | 2016 | 26593403 |
| eric-pcr genotyping of some campylobacter jejuni isolates of chicken and human origin in egypt. | the public health importance of the genus campylobacter is attributed to several species causing diarrhea in consumers. poultry and their meat are considered the most important sources of human campylobacteriosis. in this study, 287 samples from chicken (131 cloacal swabs, 39 chicken skin, 78 chicken meat, and 39 cecal parts) obtained from retail outlets as well as 246 stool swabs from gastroenteritis patients were examined. a representative number of the biochemically identified campylobacter j ... | 2015 | 26579615 |
| phenotypic characters and molecular epidemiology of campylobacter jejuni in east china. | in this study, we investigated the distribution, phenotypic and molecular typing characters of campylobacter jejuni in domestic fowl, and livestock populations in east china, to provide some reference for researches on its molecular epidemiology. a total of 1250 samples were collected from different animal sources, and c. jejuni strains were then isolated and tested for antibiotic sensitivity. antibiotics-resistance gene and pathogenic genes were detected by polymerase chain reaction. phylogenic ... | 2016 | 26565657 |
| campylobacter species isolated from poultry and humans, and their analysis using pfge in southern brazil. | campylobacteriosis is a bacterial disease transmitted to humans through ingestion of contaminated food. six hundred samples were collected, 200 from human stool samples, 200 from poultry products and 200 from poultry feces in southern brazil, and then inoculated on blood agar plates. a total of 58% of the poultry feces, 17% of the poultry meat, and 2% the of human stools tested positive for campylobacter. positive campylobacter colonies were identified as campylobacter jejuni or campylobactercol ... | 2016 | 26561789 |
| [molecular mimicry and guillain-barré syndrome]. | one-thirds of patients develop guillain-barré syndrome subsequent to campylobacter jejuni enteritis. molecular mimicry exists between c. jejuni lipo-oligosaccharides and human peripheral nerve gangliosides gm1 and gd1a. igg antibodies against gm1 or gd1a are produced in one out of 5,000 patients with c. jejuni enteritis. the autoantibodies bind to gangliosides at the nodes of ranvier in the peripheral motor nerves and activate complement in situ. this is followed by the disappearance of the volt ... | 2015 | 26560949 |
| [clinical phenotypes in guillain-barré syndrome]. | guillain-barré syndrome can be classified into several phenotypes according to the type of predominantly disturbed nerve fibers, distribution of muscular weakness, and electrophysiological and pathological findings. although several regional variants including fisher syndrome and pharyngeal-cervical-brachial weakness were initially reported in western researchers, many labor-intensive studies by japanese researchers have significantly contributed to defining and highlighting these variants. this ... | 2015 | 26560946 |
| antibiotic susceptibility and molecular characterization of campylobacter jejuni strain isolated from a guillain barré syndrome child. | | 2016 | 26558915 |
| conserved histidine residues at the ferroxidase centre of the campylobacter jejuni dps protein are not strictly required for metal binding and oxidation. | iron is an essential micronutrient for living organisms as it is involved in a broad variety of important biological processes. however, free iron inside the cell could be potentially toxic, generating hydroxyl radicals through the fenton reaction. dps (dna-binding protein from starved cells) belongs to a subfamily of ferritins and can store iron atoms inside the dodecamer. the presence of a ferroxidase centre, composed of highly conserved residues, is a signature of this protein family. in this ... | 2016 | 26555736 |
| control strategies against campylobacter at the poultry production level: biosecurity measures, feed additives and vaccination. | campylobacteriosis is the most prevalent bacterial foodborne gastroenteritis affecting humans in the european union, and ranks second in the united states only behind salmonellosis. in europe, there are about nine million cases of campylobacteriosis every year, making the disease a major public health issue. human cases are mainly caused by the zoonotic pathogen campylobacter jejuni. the main source of contamination is handling or consumption of poultry meat. poultry constitutes the main reservo ... | 2016 | 26541243 |
| susceptibility of bacteria isolated from acute gastrointestinal infections to rifaximin and other antimicrobial agents in mexico. | bacterial resistance may hamper the antimicrobial management of acute gastroenteritis. bacterial susceptibility to rifaximin, an antibiotic that achieves high fecal concentrations (up to 8,000μg/g), has not been evaluated in mexico. | 2017 | 26525276 |
| synthesis and immunodetection of 6-o-methyl-phosphoramidyl-α-d-galactose: a campylobacter jejuni antigenic determinant. | campylobacter jejuni is a leading cause of traveler's diarrhea. previously, we have shown that a c. jejuni capsule polysaccharide (cps) conjugate vaccine can fully prevent c.jejuni diarrhea in non-human primates. c.jejuni cpss are decorated with non-stoichiometric amounts of o-methyl phosphoramidate (meopn) units that are key serospecific markers. in the case of c.jejuni serotype complex hs23/36, the meopn are at positions 2 and 6 of the cps galactose (gal). we describe here the synthesis of the ... | 2015 | 26513759 |
| ex vivo proteomics of campylobacter jejuni 81-176 reveal that fabg affects fatty acid composition to alter bacterial growth fitness in the chicken gut. | campylobacter jejuni is one of the leading causes of foodborne gastrointestinal illness worldwide. here we performed ex vivo proteomic analysis of c. jejuni 81-176 in chicken, a main reservoir for human infection. at 0, 1 and 4 weeks post-infection (p.i.) with the gfp-expressing 81-176 strain, inocula were recovered from chicken ceca by cell sorting using flow cytometry. itraq-coupled 2d-lc-ms/ms analyses that detected 55 c. jejuni proteins, among which either 3 (fabg, hydb, cjj81176_0876) or 7 ... | 2016 | 26499093 |
| major contribution of the type ii beta carbonic anhydrase canb (cj0237) to the capnophilic growth phenotype of campylobacter jejuni. | campylobacter jejuni, the leading cause of human bacterial gastroenteritis, requires low environmental oxygen and high carbon dioxide for optimum growth, but the molecular basis for the carbon dioxide requirement is unclear. one factor may be inefficient conversion of gaseous co2 to bicarbonate, the required substrate of various carboxylases. two putative carbonic anhydrases (cas) are encoded in the genome of c. jejuni strain nctc 11168 (cj0229 and cj0237). here, we show that the deletion of the ... | 2016 | 26470757 |
| celery seed and related extracts with antiarthritic, antiulcer, and antimicrobial activities. | celery preparations have been used extensively for several millennia as natural therapies for acute and chronic painful or inflammatory conditions. this chapter reviews some of the biological and chemical properties of various celery preparations that have been used as natural remedies. many of these have varying activities and product qualities. a fully standardized celery preparation has been prepared known as an alcoholic extract of the seeds of a plant source derived from northern india. thi ... | 2015 | 26462366 |
| structural basis for amino-acid recognition and transmembrane signalling by tandem per-arnt-sim (tandem pas) chemoreceptor sensory domains. | chemotaxis, mediated by methyl-accepting chemotaxis protein (mcp) receptors, plays an important role in the ecology of bacterial populations. this paper presents the first crystallographic analysis of the structure and ligand-induced conformational changes of the periplasmic tandem per-arnt-sim (pas) sensing domain (ptpsd) of a characterized mcp chemoreceptor. analysis of the complex of the campylobacter jejuni tlp3 ptpsd with isoleucine (a chemoattractant) revealed that the ptpsd is a dimer in ... | 2015 | 26457436 |
| campylobacter jejuni outer membrane vesicle-associated proteolytic activity promotes bacterial invasion by mediating cleavage of intestinal epithelial cell e-cadherin and occludin. | outer membrane vesicles (omvs) play an important role in the pathogenicity of gram-negative bacteria. campylobacter jejuni produces omvs that trigger il-8, il-6, hbd-3 and tnf-α responses from t84 intestinal epithelial cells and are cytotoxic to caco-2 iecs and galleria mellonella larvae. proteomic analysis of 11168h omvs identified the presence of three proteases, htra, cj0511 and cj1365c. in this study, 11168h omvs were shown to possess proteolytic activity that was reduced by pretreatment wit ... | 2016 | 26451973 |
| molecular typing of fluoroquinolone-resistant campylobacter jejuni isolated from broilers in japan using multilocus sequence typing and pulsed-field gel electrophoresis. | fluoroquinolone-resistant campylobacter jejuni isolates from broilers in japan were characterized using multilocus sequence typing and pulsed-field gel electrophoresis (pfge) in order to elucidate the genetic relationship between these strains. forty-three of the isolates were classified into 20 sequence types and were clustered into 21 pfge types with 70% similarity. the most dominant clonal complex (cc) was cc-21 (41.9%). diverse pfge patterns were observed within the same cc, but the combined ... | 2016 | 26447604 |
| sweet talk: protein glycosylation in bacterial interaction with the host. | pathogenic bacteria encode virulent glycosyltransferases that conjugate various glycans onto substrate proteins via the n- or o-linkage. the hmw system in nontypeable haemophilus influenzae and the pgl system in campylobacter jejuni glycosylate bacterial surface or periplasmic proteins at the eukaryotic-like asn-x-ser/thr motif. the nleb effector from enterobacteria mediates arginine glcnacylation of host death-domain proteins to block inflammation, representing an atypical n-glycosylation. the ... | 2015 | 26433695 |
| campylobacter jejuni pflb is required for motility and colonisation of the chicken gastrointestinal tract. | campylobacter jejuni is the leading cause of foodborne bacterial gastroenteritis worldwide. although the mechanisms by which c. jejuni causes disease are not completely understood, the presence of functional flagella appears to be required for colonisation of the gastrointestinal tract of humans and animals. therefore much attention has been given to understanding the synthesis and role of flagella in c. jejuni. in this study we report insights into the function of pflb that is essential for cam ... | 2015 | 26423554 |
| pathogen loading from canada geese faeces in freshwater: potential risks to human health through recreational water exposure. | canada geese (branta canadensis) faeces have been shown to contain pathogenic protozoa and bacteria in numerous studies over the past 15 years. further, increases in both the canada geese populations and their ideal habitat requirements in the united states (us) translate to a greater presence of these human pathogens in public areas, such as recreational freshwater beaches. combining these factors, the potential health risk posed by canada geese faeces at freshwater beaches presents an emerging ... | 2016 | 26414207 |
| distribution of antimicrobial resistance in campylobacter strains isolated from poultry at a slaughterhouse and supermarkets in japan. | fifty strains of campylobacter jejuni/coli were detected in 108 specimens of chicken meat and organs sampled at six supermarkets and one poultry slaughterhouse (large scale) between april and october 2013 (isolation rates: 84.8% from the slaughterhouse, 29.3% from the supermarkets). 46/50 strains were successfully recovered and subjected to the e-test to examine their susceptibility to three fluoroquinolone antibacterial agents authorized for use in poultry in japan: enrofloxacin (erfx), ofloxac ... | 2015 | 26412697 |
| three transcription regulators of the nss family mediate the adaptive response induced by nitrate, nitric oxide or nitrous oxide in wolinella succinogenes. | sensing potential nitrogen-containing respiratory substrates such as nitrate, nitrite, hydroxylamine, nitric oxide (no) or nitrous oxide (n2 o) in the environment and subsequent upregulation of corresponding catabolic enzymes is essential for many microbial cells. the molecular mechanisms of such adaptive responses are, however, highly diverse in different species. here, induction of periplasmic nitrate reductase (nap), cytochrome c nitrite reductase (nrf) and cytochrome c n2 o reductase (cnos) ... | 2016 | 26395430 |
| antibacterial activities of ankaferd hemostat (abs) on shiga toxin-producing escherichia coli and other pathogens significant in foodborne diseases. | ankaferd hemostat (ankaferd blood stopper®, abs)-induced pharmacological modulation of essential erythroid proteins can cause vital erythroid aggregation via acting on fibrinogen gamma. topical endoscopic abs application is effective in the controlling of gastrointestinal (gi) system hemorrhages and/or infected gi wounds. escherichia coli o157:h7, the predominant serotype of enterohemorrhagic e. coli, is a cause of both outbreaks and sporadic cases of hemorrhagic colitis. the aim of this study i ... | 2017 | 26377624 |
| composition of human faecal microbiota in resistance to campylobacter infection. | in mice, specific species composition of gut microbiota enhances susceptibility to campylobacter jejuni but little is known about the specific composition of the human gut microbiota in providing protection from infections caused by enteropathogens. healthy adult individuals, who travelled in groups from sweden to destinations with an estimated high risk for acquisition of campylobacter infection, were enrolled. faecal samples, collected before travelling and after returning home, were cultured ... | 2016 | 26369602 |
| a novel dna-binding protein from campylobacter jejuni bacteriophage nctc12673. | we previously suggested that the double-stranded genomic dna of campylobacter jejuni bacteriophage nctc12673 was complexed with proteins. mass spectrometry of peptides obtained from tryptic digests of purified phage dna indicated that phage protein gp001 co-purified with the dna. gp001 is an acidic protein that lacks any obvious homology or conserved domains found in known dna-binding proteins. the dna-binding ability of recombinant gp001 was examined using an electrophoretic mobility shift assa ... | 2015 | 26363017 |
| seasonality of campylobacter jejuni isolates associated with human campylobacteriosis in the manawatu region, new zealand. | a 9-year time-series of genotyped human campylobacteriosis cases from the manawatu region of new zealand was used to investigate strain-type seasonality. the data were collected from 2005 to 2013 and the samples were multi-locus sequence-typed (mlst). the four most prevalent clonal complexes (ccs), consisting of 1215 isolates, were cc48, cc21, cc45 and cc61. seasonal decomposition and poisson regression with autocorrelated errors, were used to display and test for seasonality of the most prevale ... | 2016 | 26344515 |
| a probe-free four-tube real-time pcr assay for simultaneous detection of twelve enteric viruses and bacteria. | we aim to develop a multiplex real-time pcr assay to detect the most common pathogens causing community outbreaks of diarrhea. | 2015 | 26342434 |
| anti-adhesion activity of thyme (thymus vulgaris l.) extract, thyme post-distillation waste, and olive (olea europea l.) leaf extract against campylobacter jejuni on polystyrene and intestine epithelial cells. | in order to survive in food-processing environments and cause disease, campylobacter jejuni requires specific survival mechanisms, such as biofilms, which contribute to its transmission through the food chain to the human host and present a critical form of resistance to a wide variety of antimicrobials. | 2016 | 26304165 |
| structure and mechanism of an active lipid-linked oligosaccharide flippase. | the flipping of membrane-embedded lipids containing large, polar head groups is slow and energetically unfavourable, and is therefore catalysed by flippases, the mechanisms of which are unknown. a prominent example of a flipping reaction is the translocation of lipid-linked oligosaccharides that serve as donors in n-linked protein glycosylation. in campylobacter jejuni, this process is catalysed by the abc transporter pglk. here we present a mechanism of pglk-catalysed lipid-linked oligosacchari ... | 2015 | 26266984 |
| a cytolethal distending toxin gene-based multiplex pcr assay for campylobacter jejuni, c. fetus, c. coli, c. upsaliensis, c. hyointestinalis, and c. lari. | in this study, we devised a multiplex pcr assay based on the gene of cytolethal distending toxin (cdt) b subunit to simultaneously detect and discriminate campylobacter jejuni, c. fetus, c. coli, c. upsaliensis, c. hyointestinalis, and c. lari. species-specific pcr products were successfully obtained from all 38 c. jejuni, 12 c. fetus, 39 c. coli, 22 c. upsaliensis, 24 c. hyointestinalis, and 7 c. lari strains tested. on the other hand, no specific pcr products were obtained from other campyloba ... | 2016 | 26255737 |
| effects of dietary fats rich in lauric and myristic acid on performance, intestinal morphology, gut microbes, and meat quality in broilers. | this study investigated the hypothesis that dietary fats rich in lauric (c12) and myristic acid (c14) increase broiler performance and that the underlying mechanism involves antimicrobial effects on gut bacteria and changes in gut morphology. one hundred eighty 1-day-old cobb-500 broilers were allotted to 3 groups. all groups received a basal diet consisting of maize, wheat, soybean meal, and a fat source (4.5, 7.0, 7.6, and 8.0% of fat product in the diet during d 1 to 9, 10 to 17, 18 to 27, an ... | 2015 | 26240391 |
| cell surface-associated aggregation-promoting factor from lactobacillus gasseri sbt2055 facilitates host colonization and competitive exclusion of campylobacter jejuni. | campylobacter jejuni, one of the most common causes of gastroenteritis worldwide, is transmitted to humans through poultry. we previously reported that lactobacillus gasseri sbt2055 (lg2055) reduced c. jejuni infection in human epithelial cells in vitro and inhibited pathogen colonization of chickens in vivo. this suggested that the lg2055 adhesion and/or co-aggregation phenotype mediated by cell-surface aggregation-promoting factors (apfs) may be important for the competitive exclusion of c. je ... | 2015 | 26239091 |
| [detection of a disease cluster by the health authorities of stendal district due to campylobacter jejuni in a nursery after the consumption of raw milk]. | a notification from a laboratory concerning the detection of campylobacter jejuni in a 6-year-old boy initiated the investigation carried out by the health authorities from stendal district, in order to explain the morbidity rate caused by the germ. the day after the boy's pediatrician informed about a visit to a farm, which was confirmed by the head of the nursery. she told the health authorities about the consumption of raw milk during their visit at the farm.the following days more children f ... | 2015 | 26227383 |
| messenger rna expression of chicken clock gene in the response to campylobacter jejuni inoculation. | campylobacter jejuni (c. jejuni) is a leading cause of human bacterial gastroenteritis worldwide. previous research has shown that circadian rhythm plays a critical role in host response to c. jejuni colonization. the clock gene is one of the core genes regulating circadian rhythms and shows significant expression on 7 d post-c. jejuni inoculation. the objective of this study was to investigate temporal and spatial expression of chicken clock gene post-c. jejuni inoculation. cecal and splenic rn ... | 2015 | 26223235 |
| the effects of high-pressure treatments on campylobacter jejuni in ground poultry products containing polyphosphate additives. | marinades containing polyphosphates have been previously implicated in the enhanced survival of campylobacter spp. in poultry product exudates. the enhanced campylobacter survival has been attributed primarily to the ability of some polyphosphates to change the ph of the exudate to one more amenable to campylobacter. in this study a ground poultry product contaminated with a 6 strain campylobacter jejuni cocktail was utilized to determine if the efficiency of high-hydrostatic-pressure treatments ... | 2015 | 26217028 |
| role of flga for flagellar biosynthesis and biofilm formation of campylobacter jejuni nctc11168. | the complex roles of flagella in the pathogenesis of campylobacter jejuni, a major cause of worldwide foodborne diarrheal disease, are important. compared with the wild-type, an insertional mutation of the flga gene (cj0769c) demonstrated significant decrease in the biofilm formation of c. jejuni nctc11168 on major food contact surfaces, such as polystyrene, stainless steel, and borosilicate glass. the flga mutant was completely devoid of flagella and non-motile whereas the wild-type displayed t ... | 2015 | 26215271 |
| monomerization alters the dynamics of the lid region in campylobacter jejuni cstii: an md simulation study. | cstii, a bifunctional (α2,3/8) sialyltransferase from campylobacter jejuni, is a homotetramer. it has been reported that mutation of the interface residues phe121 (f121d) or tyr125 (y125q) leads to monomerization and partial loss of enzyme activity, without any change in the secondary or tertiary structures. md simulations of both tetramer and monomer, with and without bound donor substrate, were performed for the two mutants and wt to understand the reasons for partial loss of activity due to m ... | 2016 | 26208676 |
| [the significance of fucosylated glycoconjugates of human milk in nutrition of newborns and infants]. | human milk is extremely complex secretion rich in biologically active glycoconjugates including free oligosaccharides, glycoproteins, glycolipids, and glycosaminoglycans. alpha1-2-fucosylated glycoconjugates of human milk are component of the innate immune system and provide an additional defense for infants. participation of fucosylated glycotopes in the inhibition of infections caused by some bacteria and/or viruses rely on blocking of lectin-receptors of pathogen. free fucosylated glycoconjug ... | 2015 | 26206995 |
| evaluation of propidium monoazide and quantitative pcr to quantify viable campylobacter jejuni biofilm and planktonic cells in log phase and in a viable but nonculturable state. | despite being considered fragile and fastidious, campylobacter jejuni remains the leading cause of bacterial gastroenteritis in the developed world. c. jejuni survives stresses by forming biofilms or entering a viable but nonculturable (vbnc) state. to investigate the number of viable cells in samples exposed to low nutrient and temperature stress, a novel method, propidium monoazide quantitative pcr (pmaqpcr), was compared with bac light biovolume analysis and conventional plate counting for th ... | 2015 | 26197281 |
| long-lasting outbreak of erythromycin- and ciprofloxacin-resistant campylobacter jejuni subspecies jejuni from 2003 to 2013 in men who have sex with men, quebec, canada. | from january 2003 to december 2013, sexual transmission of 2 clades of campylobacter jejuni subspecies jejuni isolates resulted in a prolonged outbreak among men who have sex with men living in quebec, canada. the outbreak isolates were acquired locally and were resistant to erythromycin and ciprofloxacin. | 2015 | 26187024 |
| effects of high hydrostatic pressure on campylobacter jejuni in poultry meat. | campylobacter jejuni inactivation by high pressure processing (hpp) in poultry meat (chicken breast) was investigated. the pressure was created by high hydrostatic pressure piston-cylinder food processor. contaminated with c. jejuni (108 cfu g-1) samples of ground poultry meat were hermetically sealed in a polyamide-polyethylene bags and exposed to hpp for 9 different combinations of pressure (200 mpa, 300 mpa and 400 mpa) and time (5 min, 10 min and 15 min). quantitative bacteriological analysi ... | 2015 | 26172174 |
| an improved multiplex real-time sybr green pcr assay for analysis of 24 target genes from 16 bacterial species in fecal dna samples from patients with foodborne illnesses. | here, we developed a new version of our original screening system (rapid foodborne bacterial screening 24; rfbs24), which can simultaneously detect 24 genes of foodborne pathogens in fecal dna samples. this new version (rfbs24 ver. 5) detected all known stx2 subtypes, enterotoxigenic escherichia coli (sth genotype), and vibrio parahaemolyticus (trh2), which were not detected by the original rfbs24 assay. the detection limits of rfbs24 ver. 5 were approximately 5.6 × 10(-2)-5.6 × 10(-5) (ng dna)/ ... | 2016 | 26166499 |
| antibiotic resistance of campylobacter jejuni and c. coli isolated from children with diarrhea in thailand and japan. | a total of 29 campylobacter jejuni and c. coli strains were isolated from thai and japanese children with diarrhea using the loop-mediated isothermal amplification method. the samples were evaluated for mutations in gyra and 23s rrna in order to assess resistance against fluoroquinolones and macrolides, respectively. among the isolated strains, 9 (8 c. jejuni and 1 c. coli) were from thai children, and the other 20 (c. jejuni) were isolated from japanese children. high fluoroquinolone resistance ... | 2016 | 26166497 |
| guillain-barré syndrome. | over the past three decades much has been elucidated about the pathogenesis and clinical manifestations of guillain-barré syndrome, the most common cause of acute flaccid paralysis worldwide. cross-reactivity between surface epitopes on the bacterium campylobacter jejuni and peripheral nerve gangliosides has been shown to induce antibody-mediated axonal-type neuropathy in some patients. understanding the molecular mechanisms that cause nerve damage in these patients has led to the development of ... | 2015 | 26154996 |
| campylobacter jejuni-related cardiomyopathy: unknown entity or yet underreported? | | 2015 | 26149333 |
| human campylobacteriosis related to the consumption of raw milk sold by vending machines in italy: quantitative risk assessment based on official controls over four years. | a quantitative risk assessment (ra) model was developed to describe the risk of campylobacteriosis linked to consumption of raw milk sold in vending machines in italy. exposure assessment was based on the official microbiological records of raw milk samples from vending machines monitored by the regional veterinary authorities from 2008 to 2011, microbial growth during storage, destruction experiments, consumption frequency of raw milk, serving size, consumption preference and age of consumers. ... | 2015 | 26142145 |
| use of caprylic acid in broiler chickens: effect on campylobacter jejuni. | the effect of caprylic acid (ca) on campylobacter jejuni in chickens was evaluated using two approaches: dietary supplementation or surface treatment of chilled chicken carcasses. to analyze the dietary effect of ca, individually housed broiler chickens (n = 48) were artificially infected with c. jejuni vfu612 (10(6) colony-forming units [cfu]/bird) on the 21st and 35th days of life. dietary ca (2.5 and 5 g/kg of feed, fed throughout the entire experiment) significantly decreased c. jejuni shedd ... | 2015 | 26114373 |
| potential impact of food safety vaccines on health care costs. | foodborne pathogens continue to cause several outbreaks every year in many parts of the world. among the bacterial pathogens involved, shiga toxin-producing escherichia coli, campylobacter jejuni, and nontyphoidal salmonella species cause a significant number of human infections worldwide, resulting in a huge annual economic burden that amounts to millions of dollars in health care costs. human infections are primarily caused by the consumption of contaminated food. vaccination of food-producing ... | 2015 | 26111256 |
| multilocus sequence types of campylobacter jejuni isolates from different sources in eastern china. | campylobacter jejuni is a major food-borne pathogen that causes human gastroenteritis in many developed countries. in our study, we applied multilocus sequence typing (mlst) technology to 167 c. jejuni isolates from diverse sources in eastern china to examine their genetic diversity. mlst defined 94 sequence types (sts) belonging to 18 clonal complexes (ccs). forty-five sts from 60 isolates (36%) and 22 alleles have not been previously documented in an international database. one hundred and two ... | 2015 | 26100240 |
| characterization of campylobacter jejuni dna gyrase as the target of quinolones. | quinolones have long been used as the first-line treatment for campylobacter infections. however, an increased resistance to quinolones has raised public health concerns. the development of new quinolone-based antibiotics with high activity is critical for effective, as dna gyrase, the target of quinolones, is an essential enzyme for bacterial growth in several mechanisms. the evaluation of antibiotic activity against campylobacter jejuni largely relies on drug susceptibility tests, which requir ... | 2015 | 26096494 |
| glycosnap: a high-throughput screening methodology for engineering designer glycosylation enzymes. | the campylobacter jejuni protein glycosylation locus (pgl) encodes enzymes for asparagine-linked (n-linked) glycosylation and serves as the prototype for n-glycosylation in bacteria. this pathway has been functionally transferred into escherichia coli, thereby enabling efficient n-linked glycosylation of acceptor proteins with the c. jejuni heptasaccharide in this genetically tractable host. however, expansion of this bacterial system to encompass the vast biological diversity of potential glyca ... | 2015 | 26082213 |
| psychological comorbidity increases the risk for postinfectious ibs partly by enhanced susceptibility to develop infectious gastroenteritis. | psychological factors increase the risk to develop postinfectious ibs (pi-ibs), but the mechanisms involved are unclear. as stress affects the immune system, we investigated the potential interaction between psychological factors, the immune response against infectious gastroenteritis (ige) and the development of ige and pi-ibs in a large cohort exposed to contaminated drinking water. | 2016 | 26071133 |
| campylobacter jejuni capsular genotypes are related to guillain-barré syndrome. | in about one in a thousand cases, a campylobacter jejuni infection results in the severe polyneuropathy guillain-barré syndrome (gbs). it is established that sialylated lipo-oligosaccharides (los) of c. jejuni are a crucial virulence factor in gbs development. frequent detection of c. jejuni with sialylated los in stools derived from patients with uncomplicated enteritis implies that additional bacterial factors should be involved. to assess whether the polysaccharide capsule is a marker for gbs ... | 2015 | 26070960 |
| statistical approaches to developing a multiplex immunoassay for determining human exposure to environmental pathogens. | there are numerous pathogens that can be transmitted through water. identifying and understanding the routes and magnitude of exposure or infection to these microbial contaminants are critical to assessing and mitigating risk. conventional approaches of studying immunological responses to exposure or infection such as enzyme-linked immunosorbent assays (elisas) and other monoplex antibody-based immunoassays can be very costly, laborious, and consume large quantities of patient sample. a major li ... | 2015 | 26070441 |
| alpinia katsumadai extracts inhibit adhesion and invasion of campylobacter jejuni in animal and human foetal small intestine cell lines. | alpinia katsumadai is used in traditional chinese medicine for abdominal distention, pain, and diarrhoea. campylobacter jejuni is the most common cause of bacterial food-borne diarrhoeal illnesses worldwide. adhesion to gut epithelium is a prerequisite in its pathogenesis. the antimicrobial, cytotoxic, and anti-adhesive activities of a chemically characterised extract (see) and its residual material of hydrodistillation (hdsee-r) from a. katsumadai seeds were evaluated against c. jejuni. minimal ... | 2015 | 26058384 |
| ambiguous value of anti-ganglioside igm autoantibodies in guillain-barré syndrome and its variants. | anti-ganglioside autoantibodies of the igg type are detected in a half of patients with guillain-barré syndrome (gbs), and their detection strongly supports the diagnosis of gbs. in contrast, anti-ganglioside igm antibodies are also often detected in gbs patients, but it remains unclear whether igm antibodies indicate a diagnosis of gbs. we identified four gbs patients (3.3%) who tested positive for igm antibodies but negative for igg antibodies among 122 patients with gbs and its variants. thes ... | 2015 | 26050638 |
| constitutive expression of campylobacter jejuni truncated hemoglobin ctrhb improves the growth of escherichia coli cell under aerobic and anaerobic conditions. | bacteria hemoglobin could bind to the oxygen, transfer it from the intracellular microenvironment to the respiration process and sustain the energy for the metabolism and reproduction of cells. heterologous expression of bacteria hemoglobin gene could improve the capacity of the host on oxygen-capturing and allow it to grow even under microaerophilic condition. to develop a system based on hemoglobin to help bacteria cells overcome the oxygen shortage in fermentation, in this study, campylobacte ... | 2015 | 26047918 |
| impact of a drug-free program on broiler chicken growth performances, gut health, clostridium perfringens and campylobacter jejuni occurrences at the farm level. | the use of antimicrobial agents as feed additives in poultry production is a public health concern due to the overall increase in antimicrobial resistance. although some alternative products are commercially available, little is known on their potential impact on flock health and productivity. a prospective study involving 1.55 million birds was conducted on eight commercial broiler farms in québec, canada, to evaluate the impact of replacing antibiotic growth promoters and anticoccidial drugs b ... | 2015 | 26047674 |
| application of bacteriophages in post-harvest control of human pathogenic and food spoiling bacteria. | bacteriophages have attracted great attention for application in food biopreservation. lytic bacteriophages specific for human pathogenic bacteria can be isolated from natural sources such as animal feces or industrial wastes where the target bacteria inhabit. lytic bacteriophages have been tested in different food systems for inactivation of main food-borne pathogens including listeria monocytogenes, staphylococcus aureus, escherichia coli o157:h7, salmonella enterica, shigella spp., campylobac ... | 2016 | 26042353 |
| hyperimmunised bovine milk and whey: influence of ph and enzymatic treatments on the antigen-binding capacity of immunoglobulin g. | hyperimmunised bovine milk and whey (whole and defatted) were submitted at 37°c to different ph values (between 1 and 10) and enzymes (pepsin, trypsin and chymotrypsin) at their optimum ph and the igg immunoactivity against campylobacter jejuni was measured by means of elisa assays. | 2016 | 26041450 |
| isolation of a point mutation associated with altered expression of the cmeabc efflux pump in a multidrug-resistant campylobacter jejuni population of poultry origin. | the objective of this study was to investigate the antibiotic resistance phenotype of campylobacter jejuni isolates from a poultry flock of broiler production in spain. isolates were characterised by rflp-pcr of the flaa gene and multilocus sequence typing. minimum inhibitory concentrations of quinolones, aminoglycosides, β-lactams, tetracyclines, phenicols, macrolides and lincosamides were determined by etest. determinants of resistance and the regulatory region of the cmeabc operon were invest ... | 2015 | 27873659 |
| innate immunity to campylobacter jejuni in guillain-barré syndrome. | guillain-barré syndrome (gbs) is a postinfectious neuropathy most frequently caused by campylobacter jejuni. lipo-oligosaccharides (los), expressed by c. jejuni induce antibodies that cross-react with self-glycolipids in peripheral nerves, causing neuropathy. less than 1 in 1,000 persons infected with c. jejuni develop gbs, and the factors that determine gbs susceptibility are poorly understood. we hypothesized that these persons have a high intrinsic dendritic cell (dc) response to c. jejuni lo ... | 2015 | 26017721 |
| campylobacter. | campylobacter continues to be one of the most common bacterial causes of diarrheal illness in the united states and worldwide. infection with campylobacter causes a spectrum of diseases including acute enteritis, extraintestinal infections, and postinfectious complications. the most common species of campylobacter associated with human illness is campylobacter jejuni, but other campylobacter species can also cause human infections. this comprehensive review includes discussion of the taxonomy, c ... | 2015 | 26004643 |
| effectiveness of inactivation of foodborne pathogens during simulated home pan frying of steak, hamburger or meat strips. | in order to evaluate the effect of simulated home pan frying of raw meat and meat preparations of different animal species on the thermal inactivation of pathogens, the heat resistance (d-value) of three strains of campylobacter jejuni, escherichia coli o157:h7, salmonella spp., listeria monocytogenes and two strains of generic e. coli was validated in bhi and adjusted bhi (i.e. ph5.6 and 1.5% nacl) at 60°c. the d-values were obtained of the linear phase of the survivor curves created in ginafit ... | 2015 | 26004267 |
| campylobacter-associated hemolytic uremic syndrome associated with pulmonary-renal syndrome. | common causes of pulmonary-renal syndrome include anti-glomerular basement membrane (anti-gbm) disease anti-neutrophil cytoplasmic antibody (anca) positive vasculitis, and systemic lupus erythematosus. we describe a case of life-threatening pulmonary hemorrhage associated with campylobacter hemolytic uremic syndrome (hus), which we believe is a new disease entity. we hypothesize that the cause of this pulmonary-renal syndrome was an immunological reaction to campylobacter; and that the initiatio ... | 2016 | 26001543 |
| claudin-related intestinal diseases. | with up to 200 m(2) the human intestine is the organ with the largest absorptive surface of the body. it is lined by a single layer of epithelial cells that separates the host from the environment. the intestinal epithelium provides both, selective absorption of nutrients, ions, and water but also a highly effective barrier function which includes the first line of defense against environmental antigens. the paracellular part of this barrier function is provided by tight junction (tj) proteins, ... | 2015 | 25999319 |
| campylobacter jejuni contamination of broiler carcasses: population dynamics and genetic profiles at slaughterhouse level. | six slaughter batches deriving from six typical industrial broiler flocks were examined for the presence, quantity and genetic characteristics of contaminating campylobacter jejuni (c. jejuni) during various stages of slaughtering and carcass processing. to assess the contamination dynamics of the carcasses, the analyses were always conducted on neck-skin samples from the same pre-selected and carefully marked carcasses in each batch. the skin samples were taken sequentially at three successive ... | 2015 | 25998821 |
| perr controls oxidative stress defence and aerotolerance but not motility-associated phenotypes of campylobacter jejuni. | the foodborne bacterial pathogen campylobacter jejuni is an obligate microaerophile that is exposed to atmospheric oxygen during transmission through the food chain. survival under aerobic conditions requires the concerted control of oxidative stress systems, which in c. jejuni are intimately connected with iron metabolism via the perr and fur regulatory proteins. here, we have characterized the roles of c. jejuni perr in oxidative stress and motility phenotypes, and its regulon at the level of ... | 2015 | 25968890 |
| genetic diversity and host associations in campylobacter jejuni from human cases and broilers in 2000 and 2008. | campylobacter jejuni is an important food-borne pathogen, with a global distribution. it can colonize numerous host species, including both domestic and wild animals, but is particularly associated with birds (poultry and wild birds). for human campylobacteriosis, poultry products are deemed the most significant risk factor for acquiring infection. we conducted a genotyping and host attribution study of a large representative collection of c. jejuni isolated from humans and broilers in sweden in ... | 2015 | 25960333 |
| studies on tridecaptin b(1), a lipopeptide with activity against multidrug resistant gram-negative bacteria. | previously other groups had reported that paenibacillus polymyxa nrrl b-30507 produces srcam 37, a type iia bacteriocin with antimicrobial activity against campylobacter jejuni. genome sequencing and isolation of antimicrobial compounds from this p. polymyxa strain show that the antimicrobial activity is due to polymyxins and tridecaptin b1. the complete structural assignment, synthesis, and antimicrobial profile of tridecaptin b1 is reported, as well as the putative gene cluster responsible for ... | 2015 | 25959079 |
| reactive arthritis in relation to internal derangements of the temporomandibular joint: a case control study. | the aim of this study was to find out if reactive arthritis was involved in the aetiology of chronic closed lock of the temporomandibular joint (tmj) by looking for bacterial antigens in the synovial membrane of the tmj, and by studying the antibody serology and carriage of human leucocyte antigen (hla) b27 in patients with chronic closed lock. patients with reciprocal clicking and healthy subjects acted as controls. we studied a total of 43 consecutive patients, 15 with chronic closed lock, 13 ... | 2015 | 25957137 |
| impact of disinfectant wipes on the risk of campylobacter jejuni infection during raw chicken preparation in domestic kitchens. | in the present study, we conducted a quantitative microbial risk assessment forecasting the exposure to campylobacter jejuni contaminated surfaces during preparation of chicken fillets and how using a disinfectant-wipe intervention to clean a contaminated work area decreases the risk of infection following the preparation of raw chicken fillet in a domestic kitchen. | 2015 | 25939813 |
| microbial food safety: potential of dna extraction methods for use in diagnostic metagenomics. | the efficiency of ten widely applied dna extraction protocols was evaluated for suitability for diagnostic metagenomics. the protocols were selected based on a thorough literature study. chicken fecal samples inoculated with about 1×10(3) and 1×10(6) cfu/g campylobacter jejuni were used as a model. the evaluation was performed based on total dna yield measured by fluorometry, and quality and quantity of c. jejuni dna measured by real-time pcr. there was up to a 25-fold variance between the lowes ... | 2015 | 25937085 |
| comparison of epidemiologically linked campylobacter jejuni isolated from human and poultry sources. | campylobacter jejuni is responsible for most foodborne bacterial infections worldwide including australia. the aim of this study was to investigate a combination of typing methods in the characterization of c. jejuni isolated from clinical diarrhoeal samples (n = 20) and chicken meat (n = 26) in order to identify the source of infection and rank isolates based on their relative risk to humans. sequencing of the flaa short variable region demonstrated that 86% of clinical isolates had genotypes t ... | 2015 | 25936829 |
| the role of probiotics in the inhibition of campylobacter jejuni colonization and virulence attenuation. | campylobacter jejuni is one of the most common bacterial causes of human gastroenterocolitis worldwide, leading to diarrhea and other serious post-infectious complications. probiotics form an attractive alternative intervention strategy for most of the enteric infections. however, the role of probiotics in c. jejuni infections requires detailed investigations in order to delineate the probiotic strains that are effective against c. jejuni. although there are several biological mechanisms involve ... | 2015 | 25934376 |
| antimicrobial resistance and multilocus sequence types of finnish campylobacter jejuni isolates from multiple sources. | antimicrobial susceptibility was determined for 805 domestic campylobacter jejuni isolates obtained from broilers (n = 459), bovines (n = 120), human patients (n = 95), natural waters (n = 80), wild birds (n = 35) and zoo animals/enclosures (n = 16) with known multilocus sequence types (mlst) for 450 isolates. the minimum inhibitory concentration (mic) values for erythromycin, tetracycline, streptomycin, gentamicin and the quinolones ciprofloxacin and nalidixic acid were determined with the vetm ... | 2016 | 25917650 |
| antibiotic susceptibility profiling and virulence potential of campylobacter jejuni isolates from different sources in pakistan. | to determine antibiotic resistance patterns and virulence potential of campylobacter jejuni (c. jejuni) isolates from clinical human diarrheal infections, cattle and healthy broilers. | 2015 | 25902160 |
| molecular survey of occurrence and quantity of legionella spp., mycobacterium spp., pseudomonas aeruginosa and amoeba hosts in municipal drinking water storage tank sediments. | to examine the occurrence and quantity of potential pathogens and an indicator of microbial contamination in the sediments of municipal drinking water storage tanks (mdwsts), given the absence of such data across the united states. | 2015 | 25891145 |
| notes from the field: campylobacteriosis outbreak associated with consuming undercooked chicken liver pâté - ohio and oregon, december 2013-january 2014. | on january 8, 2014, the ohio department of health notified the oregon public health division (ophd) of campylobacteriosis in two ohio residents recently returned from oregon. the travelers reported consuming chicken liver pâté* at an oregon restaurant. on january 10, ophd received additional reports of campylobacteriosis in two persons who had consumed chicken liver pâté at another oregon restaurant. campylobacter jejuni was isolated in cultures of fecal specimens from three patients. ophd inves ... | 2015 | 25879900 |
| [effects of the environment on health of feral pigeons (columba livia)]. | we examined 80 feral pigeons and their fecal samples from two feral pigeon lofts of the "pigeon action of basel" (switzerland) for different pathogens. the tested material harbored four pathogenic agents transmissible to humans (chlamydia spp., salmonella spec., campylobacter jejuni, cryptococcus neoformans) in addition several pathogens were found which are no zoonotic agents but potentially pathogenic for the pigeons themselves, such as trichomonas gallinae, coccidia, helminths, ectoparasites ... | 2015 | 25876285 |
| the signalling molecule autoinducer-2 is not internalised in campylobacter jejuni. | bacteria recognise and constantly adjust to changing situations by sensing environmental and self-produced signals. autoinducer-2 (ai-2) is a signal molecule, found in many bacterial species and thus proposed to enable interspecies com- munication. two classes of ai-2 receptors have been identified so far. one class of ai-2 receptors sense ai-2 by a two component signalling system.the other class, the lsrb family, internalises and phosphorylates ai-2. campylobacter (c.) jejuni is known to produc ... | 2015 | 25876270 |
| changes within the intestinal flora of broilers by colonisation with campylobacter jejuni. | in most european countries human campylobacteriosis is the most important bacterial zoonotic foodborne infection. chicken meat is considered the main source of infection. since most strategies assessed so far, in reducing campylobacter colonization in chickens or in the reduction of human disease, have not been very effective, new knowledge regarding campylobacter's interaction with the host is needed. in this study we analysed fecal and cecal samples of five chicken flocks of different austrian ... | 2015 | 25876269 |
| murine infection models for the investigation of campylobacter jejuni--host interactions and pathogenicity. | despite the socioeconomic burden of campylobacteriosis, our insights into the molecular mechanisms underlying campylobacter (c.) jejuni-induced intestinal immunopathogenesis are limited.the absence and the ban of convenient murine infection models caused fundamental restrictions in campylobacter research. the development of novel and modified murine infection models in the last years has greatly contributed to our knowledge in cjejuni--host interactions and pathogenicity. novel findings revealed ... | 2015 | 25876268 |
| campylobacter jejuni: components for adherence to and invasion of eukaryotic cells. | campylobacter (c.) jejuni is the most important reported cause for bacterial diarrhoea. the infection can be accompanied by fever and abdominal cramps and in rare cases the guillain-barré syndrome or reactive arthritis can develop as a post-infection complication. several biological properties of cjejuni, e. g. motility and chemotaxis, contribute to the biological fitness of the pathogen. for this, deficiencies in the function of these features clearly reduce the pathogenicity of c. jejuni witho ... | 2015 | 25876267 |
| septic abortion caused by campylobacter jejuni bacteraemia. | a 20-year-old female patient, 14 weeks pregnant, was admitted to hospital with anamnestic and clinical features of acute pyelonephritis. clinical signs of septic abortion developed and after obstetric examination the therapy was changed to ampicillin, gentamicin and clindamycin. campylobacter jejuni was isolated from blood cultures. pathohistological findings confirmed diagnosis of purulent chorioamnionitis. after 2 weeks of ciprofloxacin administration the patient fully recovered. campylobacter ... | 2016 | 25872616 |
| loop-mediated isothermal amplification (lamp) for detection of campylobacter jejuni and c. coli in thai children with diarrhea. | campylobacter species are common causes of bacterial diarrhea, and campylobacter jejuni and c. coli are known as the predominant causative agents in humans. recent studies suggested that loop-mediated isothermal amplification (lamp) is an efficient and practical tool for rapid detection of c. jejuni and c. coli in clinical samples. we used lamp to screen 151 stool samples for campylobacter; these samples were collected in 2012 from thai children with diarrhea. the pcr method discriminated c. jej ... | 2015 | 25866115 |
| involvement of cholesterol in campylobacter jejuni cytolethal distending toxin-induced pathogenesis. | the aim of this study was to investigate whether cholesterol plays a pivotal role in cytolethal distending toxin (cdt) mediated pathogenic effects in hosts. | 2015 | 25865189 |
| application of pulsed field gel electrophoresis to type campylobacter jejuni. | pulsed field gel electrophoresis (pfge) is generally accepted as one of the most discriminatory methods available for genotyping campylobacter jejuni. pfge has been extensively used in epidemiological studies, including outbreak investigation, persistence of genotypes in a human population, environmental diversity of sporadic infection isolates, dissemination of antibiotic-resistant strains, and comparison of genotypes within and between hosts. the main purpose of this chapter is to present a wo ... | 2015 | 25862055 |
| an unconventional anaerobic membrane protein production system based on wolinella succinogenes. | in cases where membrane protein production attempts in more conventional escherichia coli-based systems have failed, a solution is to resort to a system based on the nonpathogenic epsilon-proteobacterium wolinella succinogenes. this approach has been demonstrated to be successful for structural and mechanistic analyses not only for homologous production of w. succinogenes membrane proteins but also for the heterologous production of membrane protein complexes from the human pathogens helicobacte ... | 2015 | 25857779 |
| expression of stress-related proteins in sediminibacterium sp. growing under planktonic conditions. | aggregation is a common trait of bacteria in natural and engineered biological systems. microbial aggregates, such as flocs, granules, and biofilms, are spatially heterogeneous environments. it is generally observed that by growing under aggregated conditions bacteria respond and adapt to environmental stress better than free-swimming bacteria of the same species. we performed a proteomic analysis of a strain of sediminibacterium, isolated from activated sludge, which grew planktonically in dilu ... | 2015 | 25847231 |
| the combined efficacy of carvacrol and modified atmosphere packaging on the survival of salmonella, campylobacter jejuni and lactic acid bacteria on turkey breast cutlets. | the primary objective of this study was to determine the efficacy of carvacrol in combination with modified atmosphere packaging (map) in reducing salmonella on turkey breast cutlets stored at 4 °c. in experiment i, carvacrol (0.5, 1, and 2% v/v) was applied as surface treatment and samples were stored under aerobic condition or as surface and dip treatments followed by storage in an environment of 100% carbon dioxide. the findings of the experiment i revealed the synergistic activity of carvacr ... | 2015 | 25846923 |
| effect of oxygen stress on growth and survival of clostridium perfringens, campylobacter jejuni, and listeria monocytogenes under different storage conditions. | this study investigated the growth and survival of three foodborne pathogens (clostridium perfringens, campylobacter jejuni, and listeria monocytogenes) in beef (7% fat) and nutrient broth under different oxygen levels. samples were tested under anoxic (<0.5%), microoxic (6 to 8%), and oxic (20%) conditions during storage at 7 °c for 14 days and at 22 °c for 5 days. two initial inoculum concentrations were used (1 and 2 log cfu per g of beef or per ml of broth). the results show that c. perfring ... | 2015 | 25836393 |
| campylobacter jejuni infection and ige sensitization in up to 2-year-old infants. | the "hygiene hypothesis" addresses the correlation between the occurrence of atopy and the frequency of infections in the earliest age, explaining an increase in the incidence of atopic diseases by living in good, infection-free, hygienic conditions. the aim of our study was to determine the conection between atopy and camplobacter infection, and to analyze the association between serum concentrations of total ige and campyobacter infection in relation to atopy in children up to two years. | 2015 | 25831906 |
| increased intracellular calcium level and impaired nutrient absorption are important pathogenicity traits in the chicken intestinal epithelium during campylobacter jejuni colonization. | although a high number of chickens carry campylobacter jejuni, the mechanistic action of colonization in the intestine is still poorly understood. the current study was therefore designed to investigate the effects of c. jejuni on glucose uptake, amino acids availability in digesta, and intracellular calcium [ca(2+)]i signaling in the intestines of broiler chickens. for this, we compared: control birds (n = 60) and c. jejuni-infected birds (n = 60; infected orally with 1 × 10(8) cfu of c. jejuni ... | 2015 | 25825050 |
| cytochrome c biogenesis in campylobacter jejuni requires cytochrome c6 (ccca; cj1153) to maintain apocytochrome cysteine thiols in a reduced state for haem attachment. | the microaerophilic food-borne pathogen campylobacter jejuni uses complex cytochrome-rich respiratory chains for growth and host colonisation. cytochrome c biogenesis requires haem ligation to reduced apocytochrome cysteines, catalysed by the cytochrome c synthase, ccsba. while ccsba could not be deleted, we showed that the thiol reductase dsbd and the ccsx homologue cj1207 are involved in, but not essential for, cytochromes c biogenesis. mutant phenotypic analyses and biochemical studies with p ... | 2015 | 25825009 |
| antimicrobial activity and stability of weakly acidified chlorous acid water. | the antimicrobial activity of weakly acidified chlorous acid water (wacaw) against staphylococcus aureus, non-pathogenic escherichia coli, enterohemorrhagic e. coli (ehec o157:h7), candida albicans, and spore-forming bacillus and paenibacillus species was evaluated in vitro. the antiviral activity was also examined using feline calicivirus (fcv). diluted wacaw (>100 ppm) effectively reduced the number of non-spore-forming bacteria (>4 log10 cfu reductions) within 5 min. treatment with this sanit ... | 2015 | 25817812 |