Publications

nitrogen assimilation in denitrifier bacillus azotoformans lmg 9581t.	until recently, it has not been generally known that some bacteria can contain the gene inventory for both denitrification and dissimilatory nitrate (no3-)/nitrite (no2-) reduction to ammonium (nh4+) (dnra). detailed studies of these microorganisms could shed light on the differentiating environmental drivers of both processes without interference of organism-specific variation. genome analysis of bacillus azotoformans lmg 9581t shows a remarkable redundancy of dissimilatory nitrogen reduction, ...	2017	28726125
erratum to: 'draft genome sequence of bacillus azotoformans mev2011, a (co-) denitrifying strain unable to grow with oxygen'.	[this corrects the article doi: 10.1186/1944-3277-10-4.].	2015	26430492
draft genome sequence of bacillus azotoformans mev2011, a (co-) denitrifying strain unable to grow with oxygen.	bacillus azotoformans mev2011, isolated from soil, is a microaerotolerant obligate denitrifier, which can also produce n2 by co-denitrification. oxygen is consumed but not growth-supportive. the draft genome has a size of 4.7 mb and contains key genes for both denitrification and dissimilatory nitrate reduction to ammonium.	2015	26413196
an electrogenic nitric oxide reductase.	nitric oxide reductases (nors) are members of the heme-copper oxidase superfamily that reduce nitric oxide (no) to nitrous oxide (n₂o). in contrast to the proton-pumping cytochrome oxidases, nors studied so far have neither been implicated in proton pumping nor have they been experimentally established as electrogenic. the copper-a-dependent nor from bacillus azotoformans uses cytochrome c₅₅₁ as electron donor but lacks menaquinol activity, in contrast to our earlier report (suharti et al., 2001 ...	2015	26149211
bacillus salitolerans sp. nov., a novel bacterium isolated from a salt mine in xinjiang province, china.	a novel aerobic bacterium, kc1(t), was isolated from a salt mine in kuche county, xinjiang province, china. cells were observed to be gram-positive, rod-shaped, endospore-forming and motile with flagella. strain kc1(t) was found to grow at 25-45 °c (optimum 37 °c), ph 6.5-9.0 (optimum 8.0) and nacl 0-10 % (v/v) (optimum 4 %). the major fatty acids were identified as anteiso-c15:0 and anteiso-c17:0. menaquinone-7 (mk-7) was found to be the predominant isoprenoid quinone. the cell-wall diamino aci ...	2015	26076748
draft genome sequence of bacillus azotoformans mev2011, a (co-) denitrifying strain unable to grow with oxygen.	bacillus azotoformans mev2011, isolated from soil, is a microaerotolerant obligate denitrifier, which can also produce n2 by co-denitrification. oxygen is consumed but not growth-supportive. the draft genome has a size of 4.7 mb and contains key genes for both denitrification and dissimilatory nitrate reduction to ammonium.	2014	25685261
experimental investigation of microbiologically influenced corrosion of selected steels in sugarcane juice environment.	in the current study, ferritic stainless grades aisi 439 and aisi 444 were investigated as possible construction materials for machinery and equipment in the cane-sugar industry. their performance in corrosive cane-sugar juice environment was compared with the presently used low carbon steel aisi 1010 and austenitic stainless steel aisi 304. the tafel plot electrochemical technique was used to evaluate general corrosion performance. microbiologically influenced corrosion (mic) behaviour in sugar ...	2013	23764955
structure of microbial communities performing the simultaneous reduction of fe(ii)edta.no2- and fe(iii)edta -.	biodenox is a combined physicochemical and biological process for the removal of nitrogen oxides (nox) from flue gas. in the present study, two anaerobic bioreactors performing biodenox were run consecutively (run-1 and run-2) at a dilution rate of 0.01 h(-1) with fe(ii)edta.no(2-) and fe(iii)edta(-) as electron acceptors and ethanol as electron donor. the measured protein concentration of the reactor biomass of both runs was 120 mg/l. different molecular methods were used to determine the ident ...	2006	16957895
identification of nif genes in n2-fixing bacterial strains isolated from rice fields along the yangtze river plain.	the aim of this research was to identify nifh and nifhdkye ' genes in twenty strains of n2-fixing heterotrophic bacteria isolated from rice fields in the yangtze river plain. southern hybridization of the total dna from each strain was performed with the klebsiella pneumoniae nifhdkye ' gene probe (6.2 kb eco ri fragment from psa30) and the azospirillum brasilense nifh gene probe (0.6 kb eco ri-hin diii fragment from phu8). we found that eco ri fragments of total dna from aeromonas hydrophila hy ...	2006	16463319
membrane-bound denitrification in the gram-positive bacterium bacillus azotoformans.	gram+ bacteria are capable of complete denitrification just like gram- (gram-negative) bacteria. however, gram+ (gram-positive) bacteria have a very small periplasmic-like space. this leads to the question of whether those enzymes and electron carriers involved in denitrification, which are normally located in the periplasmic space in gram- bacteria, are located in the periplasmic-like space in gram+ bacteria or have been modified as membrane-bound proteins. using bacillus azotoformans as a gram ...	2005	15667284
characterization of microbial communities removing nitrogen oxides from flue gas: the biodenox process.	biodenox is an integrated physicochemical and biological process for the removal of nitrogen oxides (nox) from flue gases. in this process, the flue gas is purged through a scrubber containing a solution of fe(ii)edta2-, which binds the nox to form an fe(ii)edta.no2- complex. subsequently, this complex is reduced in the bioreactor to dinitrogen by microbial denitrification. fe(ii)edta2-, which is oxidized to fe(iii)edta- by oxygen in the flue gas, is regenerated by microbial iron reduction. in t ...	2005	16204556
no reductase from bacillus azotoformans is a bifunctional enzyme accepting electrons from menaquinol and a specific endogenous membrane-bound cytochrome c551.	bacillus azotoformans is a gram-positive denitrifying soil bacterium, which is capable of respiring nitrate, nitrite, nitric oxide, and nitrous oxide under anaerobic conditions. it contains a unique menaquinol-dependent nitric oxide reductase (qcu(a)nor) with a cu(a) center in its small subunit. the qcu(a)nor exhibits menaquinol-dependent no reductase activity, whereas reduced horse heart cytochrome c was inactive. here we describe the purification of three membrane-bound c cytochromes from b. a ...	2004	15491156
two co molecules can bind concomitantly at the diiron site of no reductase from bacillus azotoformans.	co complexes formed in reduced nitric oxide reductase from bacillus azotoformans were investigated with resonance raman and ftir techniques. these experiments shows the presence of two nu(c-o) bands, one at approximately 1970 cm-1 assigned to the heme-co complex, and one at approximately 2070 cm-1 from the non-heme iron, febco. at cryogenic temperatures, the heme-co complex adopts a semi-bridging configuration with feb which decreases its stretching frequency to approximately 1910 cm-1 and decre ...	2004	15563131
a novel copper a containing menaquinol no reductase from bacillus azotoformans.	the molecular biology and biochemistry of denitrification in gram-negative bacteria has been studied extensively. however, little is known about this process in gram-positive bacteria. we have purified the no reductase from the cytoplasmic membrane of the gram-positive bacterium bacillus azotoformans. the purified enzyme consists of two subunits with apparent molecular masses of 16 and 40 kda based on sds-page. analytical and spectroscopic determinations revealed the presence of one non-heme iro ...	2001	11327887
mobilization of broad host range plasmid from pseudomonas putida to established biofilm of bacillus azotoformans. i. experiments.	a strain of pseudomonas putida harboring plasmids rk2 and pdlb101 was exposed to a pure culture biofilm of bacillus azotoformans grown in a rotating annular reactor under three different concentrations of the limiting nutrient, succinate. experimental results demonstrated that the broad host range rsf1010 derivative pdlb101 was transferred to and expressed by b. azotoformans. at the lower concentrations, donor mediated plasmid transfer increased with increasing nutrient levels, but the highest n ...	1998	10099203
mobilization of broad host range plasmid from pseudomonas putida to established biofilm of bacillus azotoformans. ii. modeling.	a strain of pseudomonas putida that harbors plasmids rk2 and pdlb101 was exposed to a pure culture biofilm of bacillus azotoformans grown in a rotating annular reactor. transfer of the rk2 mobilizable pdlb101 plasmid to b. azotoformans was monitored over a 4-day period. experimental results demonstrated that the broad host range, rsf1010 derivative pdlb101 was transferred to and expressed by b. azotoformans. in the companion article to this work, the rate of plasmid transfer was quantified as a ...	1998	10099204
criteria and methodology for identifying respiratory denitrifiers.	respiratory denitrification is not always adequately established when bacteria are characterized. we have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that n(inf2) or n(inf2)o is produced from nitrate or nitrite and that this reduction is coupled to a growth yield increase. microorganisms were cultured in sealed tubes under a helium headspace and in the presence of 0, 2, 4, 7, and 10 mm nitrate or nitrite. a ...	1995	16534960
two structurally different cytochromes c from bacillus azotoformans: on the evolution of gram-positive bacteria.	c-552 and split-alpha c-555 cytochromes from bacillus azotoformans are classified on the basis of partial sequence information. the haem-containing polypeptides are postulated to be structurally equivalent to small ic and id subclass cytochromes found in purple bacteria.	1991	1646020
immunological identification and distribution of dissimilatory heme cd1 and nonheme copper nitrite reductases in denitrifying bacteria.	polyclonal antibodies were used to identify heme or copper nitrite reductases in the following groups: 23 taxonomically diverse denitrifiers from culture collections, 100 numerically dominant denitrifiers from geographically diverse environments, and 51 denitrifiers from a culture collection not selected for denitrification. antisera were raised against heme nitrite reductases from pseudomonas aeruginosa and pseudomonas stutzeri and against copper nitrite reductase from achromobacter cycloclaste ...	1989	2624465
[morphological, physiological and taxonomic studies of bacillus azotoformans].	seventeen strains of the new species bacillus azotoformans were isolated by enrichment culture in peptone broth inoculated with pasteurized soil and then incubated under n2o at 32 degrees c. the bacterium is a gram-negative rod, motile with peritrichous flagella, which produces oval spores without exosporia in swollen sporangia. however, the cells have thick walls, mesosomes, and persistent septa characteristic of gram-positive bacteria. the bacterium lacks fermentative activity, does not attack ...	1978	657012
[a new, sporulating, denitrifying, mesophilic bacterium: bacillus azotoformans n. sp. (author's transl)].	the described bacterium was isolated by enrichment culture in peptone broth inoculated with garden soil, pasteurized and then put to incubate under n2o at 32 degrees. it is a gram-negative rod, motile with peritrichous flagella, and producing oval spores without exosporium in swollen sporangia. however, cells have the thick walls, mesosomes and persistant septa characteristic of gram-positive bacteria. it lacks fermentative activity, does not attack carbohydrates, has complex growth requirements ...	1976	1020872