Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| evidence for thyroid hormone receptors in uterine nuclei. | nuclear preparations from the rat uterus contain specific, high affinity binding sites for t3, with the properties expected of a thyroid hormone receptor. the kd value for the binding of t3 to these sites is 9.3 +/- 0.1 x 10(-10) m and the maximum number of binding sites is 0.11 +/- 0.02 pmoles t3 bound per mg of dna. competition experiments with triac, d-t3, t4, and rt3 compared to l-t3 revealed relative affinities of 87%, 22%, 15%, and 1.6% respectively. t3 binding sites solubilized from uteri ... | 1983 | 6298566 |
| chronic effect of tsh on human thyroid tissue in organ culture. | the chronic effect of tsh on thyroidal camp concentrations and release of thyroid hormones was investigated using human thyroid tissue in organ culture. normal human thyroid slices were placed in ham's f-10 synthetic culture medium in falcon organ tissue culture dishes, and incubated at 37 degrees in a humidified atmosphere of 5% co2 in air. medium was changed everyday and daily t3 or t4 release was determined using concentration of t3 or t4 in the medium. after incubation, slices were transferr ... | 1983 | 6298795 |
| effects of a polybrominated biphenyl mixture in the rat and mouse. i. six-month exposure. | a 1973 environmental accident in michigan resulted in exposure of humans via the food chain to polybrominated biphenyl (pbb). to better characterize the toxicity of the halogenated polycyclic aromatic hydrocarbon class of chemicals, rodents were dosed with pbb and their target organs examined for morphological, histological, biochemical, and selected endocrine changes. male and female rats and mice were given 125 po doses of pbb over a 6-month period at 0.1, 0.3, 1.0, 3.0, and 10.0 mg/kg of body ... | 1983 | 6302948 |
| structural features of very fast sedimenting dna formed by gene 49 defective t4. | very fast sedimenting dna (vfs dna) of t4 phage, which is formed by infection with a mutant in gene 49, was examined by electron microscopy after mild treatment with dnase i. it showed y-shaped, branched strands in addition to linear strands. each branch contained a single-stranded interruption about 60 nucleotides long at its proximal end. the average number of branches per t4 dna unit was close to the average number of sites susceptible to gene 49 nuclease. both numbers were consistently chang ... | 1983 | 6302980 |
| binding of thyroid hormones to isolated hepatic nuclei from rana catesbeiana tadpoles. | this study is concerned with the characteristics of thyroid hormone binding to isolated hepatic nuclei from premetamorphic tadpoles. conditions essential for nuclear stability and/or demonstration of saturable binding included 220-mosmol buffers containing 0.1 mm zncl2 and removal of most of the melanin granules; binding of t4 and t3 to melanin was significant, but unsaturable. scatchard analysis of [125i]t3 binding to nuclei in the presence of increasing concentrations of t3 revealed the presen ... | 1983 | 6303753 |
| tsh secretion and regulation in endemic goiter and endemic cretinism. | in endemic goiter patients living in a chronic iodide deficiency area (less than 50ug/day) tsh basal levels and the tsh response to trh is significantly higher (31 +/- 8uu/ml) than in normal controls (10 +/- 4uu/ml). there is a negative correlation between levels of iodine intake and serum tsh concentration and thyrotropin is thus increased when iodine decreased. also it was shown that serum tsh levels correlate inversely with the serum concentration of t4 (r=0.69) but not with the usually norma ... | 1983 | 6304776 |
| effects of kclo4 in propylthiouracil-hypothyroid rats. | in this study we investigated further the antigoitrogenic effect of clo4 in rats on a low iodine diet (lid) and 6-propyl-2-thiouracil (ptu). the thyroid weight of rats on the mixed goitrogen was initially similar to that of animals on ptu, decreasing to values obtained in rats treated with clo4 alone by 10 days. despite the differences in thyroid weight, rats treated during an identical period with ptu or mixed goitrogen develop hypothyroidism to a comparable degree as far as can be assessed by ... | 1983 | 6305078 |
| hypothyroidism associated with positive results of the perchlorate discharge test in elderly patients. | in 172 patients over the age of 55, determinations were made of thyrotropin concentrations in plasma. although they showed such symptoms or signs of hypothyroidism as puffy face, dry skin, general malaise, cold intolerance, or constipation, there were no clinical indications of overt hypothyroidism. all the patients were apparently in a normal nutritional state, without any specific medication that might cause thyroid abnormalities. seventeen (six men and 11 women, 9.9 percent) of the 172 patien ... | 1983 | 6305191 |
| familial resistance to thyroid hormone associated with decreased transport across the plasma membrane. | resistance to thyroid hormone associated with a defect in hormone transport across the plasma membrane occurred in a 74-year-old eumetabolic woman. she had marked elevations in the serum levels of total thyroxine (t4), free t4, and reverse triiodothyronine (rt3). levels of t3 were only minimally increased. the hyperthyroxinemia was thyrotrophin (tsh) dependent, but was not due to an alteration in the binding of t4 to serum proteins. refractoriness to thyroxine was only partial, as indicated by t ... | 1983 | 6305243 |
| thyroxine action on the rat liver nuclear thyroid-hormone receptors. binding of thyroxine to the nuclear non-histone protein and induction of mitochondrial alpha-glycerophosphate dehydrogenase activity. | the possibility that thyroxine (t4) itself exerts the hormonal effect in vivo on the rat liver nuclear receptor was studied with the aid of iopanoic acid (iop), an inhibitor of the conversion of t4 into tri-iodothyronine (t3). after administration of 2.4 micrograms of t4/100 g body weight to hypothyroid rats for 7 days, t4 and t3 concentrations in serum and in the liver nuclear non-histone protein (nhp) were all increased to the hyperthyroid range. hepatic mitochondrial alpha-glycerophosphate de ... | 1983 | 6305340 |
| studies on dna replication in the bacteriophage t4 in vitro system. | 1983 | 6305581 | |
| age affects the generation of serum thyronine-binding protein by rats fed a low protein-high carbohydrate diet. | the feeding of a low protein-high carbohydrate (pm) diet to young (8-week-old) rats induces the production of serum thyronine-binding globulin. the effect of age on this process was studied in older (5-month-old) rats. in contrast to the findings in 8-week-old rats, no significant differences in the serum concentrations of t3 and free t3 and the percentage of free t3 (equilibrium dialysis) were observed between the 5-month-old rats fed the control diet and those fed the pm diet. there were also ... | 1983 | 6305635 |
| improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication. | improved expression vectors have been constructed which are derived from runaway-replication mutants of plasmid r1 and carry the strong leftward promoter (pl) of bacteriophage lambda. the activity of this promoter is controlled by a temperature-sensitive repressor, product of the phage gene ci cloned on a compatible plasmid. heat induction leads to amplification of the plasmid copy number and at the same time turns on the promoter. at a short distance downstream from the promoter, unique ecori, ... | 1983 | 6305768 |
| the role of 99mtc pertechnetate uptake in the evaluation of thyroid function. | to investigate the usefulness of the 20 min 99mtc-pertechnetate uptake test, the records of 246 consecutive patients were reviewed. of these, 192 patients (151 females, 41 males; 10 weeks to 78 years) had at least one year clinical follow-up or a confirmed diagnosis by biopsy or surgery and were included in our study. in these patients, the 99mtc pertechnetate uptake and hormonal values (t3 resin uptake, t4 ria, t-index) were obtained. these results were then compared to the clinical diagnosis a ... | 1983 | 6305864 |
| a substrate radical intermediate in the reaction between ribonucleotide reductase from escherichia coli and 2'-azido-2'-deoxynucleoside diphosphates. | the b2 subunit of ribonucleotide reductase from escherichia coli contains a tyrosine radical which is essential for enzyme activity. in the reaction between ribonucleotide reductase and the substrate analogue 2'-azido-2'-deoxycytidine 5'-diphosphate a new transient radical is formed. the epr characteristics of this new radical species are consistent with a localization of the unpaired electron at the sugar moiety of the nucleotide. the radical shows hyperfine couplings to a hydrogen and a nitrog ... | 1983 | 6305969 |
| knotting of the circular duplex dna by type ii dna topoisomerase from drosophila melanogaster. | dna molecules with topological knots can be detected in a reaction mixture of circular dna and type ii dna topoisomerase purified from the embryos of drosophila melanogaster. similar to the dna knotting reaction by bacteriophage t4-induced topoisomerase (liu, l. f., liu, c. c., and alberts, b. m. (1980) cell 19, 697-707), the knotting reaction by the drosophila topoisomerase ii requires a high ratio of enzyme/dna. however, they differ in the following aspects: 1) the knotting reaction by the dro ... | 1983 | 6305983 |
| double strand dna cleavage by type ii dna topoisomerase from drosophila melanogaster. | the purified type ii dna topoisomerase from the embryos of drosophila melanogaster exists in its native form as a dimer of 170,000-dalton polypeptides. in addition to the 170,000-dalton polypeptides, 3 polypeptides with molecular weights of 151,000, 141,000, and 132,000 were resolved when the enzyme was analyzed by electrophoresis under denaturing conditions. all four polypeptides can participate in the topoisomerase cleavage reaction and form covalent complexes with the cleaved dna. furthermore ... | 1983 | 6305984 |
| site-specific cleavage of bacteriophage t4 dna associated with the absence of gene 46 product function. | a plasmid containing a copy of the late gene 23 was cleaved at two specific locations after bacteriophage t4 infection. cleavage at the major site, which is at the 3' end of gene 23, was detected only in the absence of gene 46 product function and was independent of the state of modification of cytosine residues. cutting of plasmid (cytosine-containing) dna at this site was independent of phage dna replication and late transcription functions. a second cleavage site, in vector dna, was also mapp ... | 1983 | 6306283 |
| pituitary nuclear triiodothyronine receptors during development in the rat. | studies were undertaken to measure the pituitary nuclear triiodothyronine (t3) binding capacity (bc) during development in the rat. bc was measured in 0.4 m nacl-solubilized receptors in 5-, 14-, 20-, 27-, 30-, 40-, 50-day-old rats and in adult animals. results indicate that bc is lower in 5-day-old rats than in adult animals (0.397 +/- 0.02 vs. 0.797 +/- 0.06 pmol t3/mg protein) (mean +/- se) (p less than 0.01). hypothyroidism, induced during the neonatal period or in the adult animal, results ... | 1983 | 6307060 |
| mononuclear cells in malignant and benign human breast tissue. | the mononuclear cell infiltrate in benign and malignant breast tissue was studied using monoclonal antibodies with an immunoenzymatic technique. infiltrating duct carcinomas contained a mononuclear cell infiltrate made up mostly of t lymphocytes, with minor b-cell and macrophage populations. the most preponderant t-cell subset was the t8, cytotoxic-suppressor cell. some tumors contained t6-positive cells, possibly immature t cells. in contrast, stromal lymphocytes in benign fibrous mastopathy we ... | 1983 | 6307213 |
| normal ontogeny of alpha 1-adrenergic receptor in rat liver is thyroid hormone dependent. | postnatal ontogeny of rat liver alpha 1-adrenergic receptor was examined using alpha 1-specific radioligand [3h]prazosin in control and propylthiouracil-treated congenital hypothyroid rats at various ages. partially purified rat liver membranes prepared by the neville method had 8-fold purification of 5'-nucleotidase from the crude homogenates from postnatal day 5 to adulthood. [3h]prazosin binding was typical of an alpha 1-adrenergic receptor, and (-)epinephrine affinity for the [3h]prazosin-bi ... | 1983 | 6307646 |
| molecular cloning of a functional dam+ gene coding for phage t4 dna adenine methylase. | phages t2 and t4 induce synthesis of a dna-adenine methylase which is coded for by a phage gene, dam+. these enzymes methylate adenine residues in specific sequences which include g-a-t-c, the methylation site of the host escherichia coli dam+ methylase. methylation of g-a-t-c to g-m6a-t-c protects the site against cleavage by the mboi restriction nuclease. we have taken advantage of this property to enrich and screen for transformants which contain a cloned, functional t4 dam+ gene. these recom ... | 1983 | 6307815 |
| effects of glucocorticoids on circulating concentrations of thyroxine (t4) and triiodothyronine (t3) and on peripheral monodeiodination in pre- and post-hatching chickens. | the administration of either glucocorticoids (dexamethasone or corticosterone) or adrenocorticotropic hormone (acth) to chicken embryos was followed by increase in the circulating concentration of triiodothyronine (t3), the t3 to thyroxine (t4) ratio and the activity of liver t4-5' monodeiodinase. no consistent changes in plasma concentrations of t4 or gh were observed. in post-hatching chicks, corticosterone and dexamethasone depressed the circulating concentrations of both t4 and t3. iopanoc a ... | 1983 | 6307846 |
| [behavior of the principal protein hormones in normal and leukemic leukocytes]. | gh, lh, insulin and glucagon patterns were studied in the peripheral leukocytes of normal subjects (granulocytes and lymphocytes separated on a ficoll-hypaque gradient) and leukaemic patients (cml, aml, cll, and all), using a double antibody ria on whole cells. the uptake of 125i-labelled insulin and gh by these cells was also assessed. the results showed that in leukaemia, particularly cll, all and aml, though not in cml, there was a constant reduction in hormone values, plus depressed gh and i ... | 1983 | 6309118 |
| nuclear thyroxine and triiodothyronine receptors in human mononuclear cells in diabetes mellitus. | the number of nuclear thyroxine (t4) or triiodothyronine (t3) receptors and the serum values of thyroxine, triiodothyronine, reverse triiodothyronine and tsh were investigated in 13 patients with type 1 (insulin-dependent) diabetes (group 1), in 10 patients with type 2 (non-insulin-dependent) diabetes (group 2) and in age and weight matched non-diabetic subjects. all patients were clinically euthyroid, although serum t3 was low and reverse t3 high in group 1 compared with the non-diabetic subjec ... | 1983 | 6309586 |
| thyroid hormone and immunological studies in endemic goiter. | iodized oil (1 ml im) was given to 58 goitrous patients from a mildly iodine-deficient area in greece. goiter size, urinary iodine, and serum t4, t3ru, t3, rt3, tsh, thyroxine-binding globulin (tbg), and thyroid autoantibodies were measured before and 1, 3, and 6 months after the injection. goiter size decreased. serum t4 remained relatively constant, but tbg decreased and therefore t3ru and fti increased. serum t3 and rt3 initially decreased (p less than 0.001) and then increased at the sixth m ... | 1983 | 6309889 |
| immunoregulatory human t lymphocytes triggered as a consequence of viral infection: clonal analysis of helper, suppressor inducer and suppressor effector cell populations. | the regulatory functions of a series of human t cell clones specific for an autologous epstein-barr virus transformed b lymphoblastoid cell line were examined. two t4+ t cell clones, termed at4ii and at4iv, and one t8+ clone, at8iii, were maintained in culture for greater than or equal to 9 months and were characterized in detail. both t4+ clones provided helper function for autologous b cell immunoglobulin production when added to unstimulated peripheral blood mononuclear cells. in addition, th ... | 1983 | 6309959 |
| detection and mapping of homologous, repeated and amplified dna sequences by dna renaturation in agarose gels. | a new molecular hybridization approach to the analysis of complex genomes has been developed. tracer and driver dnas were digested with the same restriction enzyme(s), and tracer dna was labeled with 32p using t4 dna polymerase. tracer dna was mixed with an excess amount of driver, and the mixture was electrophoresed in an agarose gel. following electrophoresis, dna was alkali-denatured in situ and allowed to reanneal in the gel, so that tracer dna fragments could hybridize to the driver only wh ... | 1983 | 6310499 |
| malignant salivary gland tumors of the paranasal sinuses and nasal cavity. | of a total of 122 patients with minor salivary gland tumors of the nose and paranasal sinuses, 66 patients were identified who had had their therapy at the university of texas m.d. anderson hospital and tumor institute, houston. adenoid cystic carcinomas and adenocarcinomas were the most frequent tumors. eighty percent of the patients were treated for relatively advanced (t3 and t4) cancers. low-grade and high-grade carcinomas could be identified in the two groups studied, and the pathologic gra ... | 1983 | 6311147 |
| nuclear thyroid hormone receptor in the rat uterus. | the uterus may be a target organ for t3 action. the present study was done to determine whether uterine nuclei contain receptors for t3 (t3r). methods were established for the measurement of t3r concentrations in preparations of rat uterine nuclei. the liver was studied concomitantly as a tissue known to contain nuclear t3r. nuclear fractions were prepared by established procedures and extracted in buffer containing 0.5 m kcl. specific [125i]t3 binding in the nuclear extract was analyzed by scat ... | 1983 | 6311523 |
| thyroxine binding and cellular metabolism of thyroxine in mononuclear blood cells from patients with anorexia nervosa. | nuclear binding of thyroxine (t4), cellular deiodination of t4 and nuclear accumulation of endogenous tri-iodothyronine (t3) were investigated in mononuclear blood cells from eight patients with anorexia nervosa. although the patients were euthyroid, serum t3 was depressed and serum reverse t3 increased. the nuclear maximal specific binding capacity (msbc) for t4 in cells from patients with anorexia nervosa was increased (2.4 x 10(-16) mol t4/10 micrograms dna, p less than 0.01) as compared to t ... | 1983 | 6311930 |
| specific 3,3',5'-triiodothyronine (reverse t3) binding sites on rat liver plasma membranes: comparison with thyroxine (t4) binding sites. | the binding characteristics of thyroxine (t4), triiodothyronine (t3), and reverse t3 (rt3) to rat liver plasma membranes (rlpm) were examined to explore the interactions of thyroid hormones with cell surface receptors. scatchard analysis suggested that all three ligands bound to two classes of binding sites. the high affinity rt3 binding sites appeared to be distinct from the high affinity t4 sites, on the basis of differing optimum physicochemical conditions for binding, and analog displacement ... | 1983 | 6312037 |
| structural analysis of t4 dna helix destabilizing protein (gp32 i) crystal by electron microscopy. | low dose electron diffraction and imaging techniques have been applied to the study of the crystalline structure of gp32*i, a dna helix destabilizing protein derived from bacteriophage t4 gene 32 protein. a quantitative analysis of intensities from electron diffraction patterns from tilted, multilayered gp32*i crystal has provided the unit cell thickness of the crystal. the three-dimensional phases indicate that the space group p2(1)2(1)2. by taking into account the unit cell volume and the solv ... | 1983 | 6312050 |
| alteration of peripheral beta-adrenergic responsiveness in fasted rats. | total food deprivation for 72 hrs (3 day fast) in female rats resulted in a reduction in serum thyroid hormones as well as a reduced peripheral beta-adrenergic responsiveness to isoproterenol. food deprivation for 48 or 72 hrs significantly decreased both serum t3 and t4 values as compared to non-fasted controls. there were no significant differences in either t3 or t4 levels as a result of a 24 hr fast. rats deprived of food for 72 hr had significantly smaller increases in oxygen consumption, c ... | 1983 | 6312234 |
| escherichia coli phage t4 topoisomerase. | 1983 | 6312256 | |
| viability of lambda phages carrying a perfect palindrome in the absence of recombination nucleases. | in escherichia coli in vitro constructions of perfect palindromes larger than 30 base pairs (bp) long have in general been unstable. a perfect palindrome has the unique possibility of forming a cruciform structure, and it is this feature which probably results in its instability. negative supercoiling favours the formation of the cruciform conformation, which in turn causes the molecule to relax. this relaxation may render replicons containing large perfect palindromes inviable. an alternative h ... | 1983 | 6312322 |
| thyroid hormone binding to adult rat alveolar type ii cells. an autoradiographic study. | hypothyroid adult rats were injected intravenously with [3, 5, 3'-125i]triiodothyronine (t3) or [125i]thyroxine (t4). others were similarly treated except for the addition of a 500-fold excess of the appropriate unlabeled hormone. light microscopic autoradiography of semithin sections of lung from these animals demonstrated labeling localized over alveolar parenchymal tissue. analysis of the labeling studies of type ii cells revealed high affinity, low capacity binding of t3 and, to a much lesse ... | 1983 | 6313340 |
| characterization of nuclear triiodothyronine (t3) and tetraiodothyronine (t4) binding in developing brain tissue. | the main objective of this study was to characterize nuclear t3 and t4 binding in the developing rat brain. more specifically, we sought to determine (a) whether t3 and t4 bind to the same nuclear receptor, (b) whether there are multiple forms of nuclear t3 or t4 receptors, and (c) whether the above parameters are similar in nuclei of cerebral hemispheres and cerebellum of developing rat brain. from in vivo and in vitro binding experiments utilizing gel filtration techniques, we have shown that ... | 1983 | 6313450 |
| recombination between bacteriophage t4 and plasmid pbr322 molecules containing cloned t4 dna. | reciprocal recombination between t4 dna cloned in plasmid pbr322 and homologous sequences in bacteriophage t4 genomes leads to integration of complete plasmid molecules into phage genomes. indirect evidence of this integration comes from two kinds of experiments. packaging of pbr322 dna into mature phage particles can be detected by a dna--dna hybridization assay only when a t4 restriction fragment is cloned in the plasmid. the density of the pbr322 dna synthesized after phage infection is also ... | 1983 | 6313944 |
| primary structure and genetic organization of phage t4 dna ligase. | the primary structure of phage t4 dna ligase has been determined by dna sequencing of a cloned restriction fragment containing its gene, and partial amino acid sequence analysis of the protein. the molecule has a mr of 55,230, and contains 487 amino acids. the dna sequence may also encode all of one and parts of two other, hitherto unidentified, t4 proteins. the four genes are closely packed, with overlaps between terminator and initiator codons of adjacent genes. potential terminator and promot ... | 1983 | 6314278 |
| neostigmine antagonism of succinylcholine phase ii block: a comparison with pancuronium. | to assess the efficacy of neostigmine antagonism of succinylcholine phase ii block, succinylcholine infusions were given to 17 patients for durations varying from 44 to 192 minutes. a control group (17 patients) received a pancuronium infusion for similar times. ninety per cent neuromuscular block was maintained in these two groups by adjustment of the infusion rates and, in a third group, with intermittent doses of pancuronium. neuromuscular transmission was monitored with train-of-four stimula ... | 1983 | 6315193 |
| the effects of food deprivation on beta-adrenergic responsiveness in male rats. | male rats were deprived of food for varying lengths of time (0-96 h) and their responses to beta-adrenergic stimulation with isoproterenol were tested. food deprivation for 48 or 96 h attenuated the increase in tail skin temperature normally seen following administration of isoproterenol. the degree of attenuation was dependent on the duration of the deprivation period. rats deprived of food for 96 h and then refed for 48, 96, or 144 h showed a return of tail skin temperature response to near no ... | 1983 | 6315202 |
| identification of bacteriophage t4 gene 60 product and a role for this protein in dna topoisomerase. | bacteriophage t4 dna topoisomerase has been isolated and shown to contain the proteins coded by the dna-delay genes 39 and 52 (liu, l. f., liu, c.-c., and alberts, b. m. (1979) nature (lond.) 281, 456-461 and stetler, g. l., king, g. j., and huang, w. m. (1979) proc. natl. acad. sci. u. s. a. 76, 3737-3741). from complementation measurements in vitro and from earlier genetic evidence, these workers suggested that the product of gene 60 (p60) was also a component of the dna topoisomerase complex. ... | 1983 | 6296073 |
| purification and properties of t4 phage thymidylate synthetase produced by the cloned gene in an amplification vector. | we have introduced the t4 thymidylate synthetase gene, resident in a 2.7-kilobase ecori restriction fragment, into an amplification plasmid, pkc30. by regulating expression of this gene from the phage lambda pl promoter within pkc30 in a thya host containing a temperature-sensitive lambda repressor, the t4 synthetase could be amplified about 200-fold over that after t4 infection. at this stage, a 20-fold purification was required to obtain homogeneous enzyme, mainly by an affinity column procedu ... | 1983 | 6296121 |
| temperature dependence of the electronic spin-lattice relaxation time in a 2-iron-2-sulphur model complex. | the complex [fe2s2(s2-o-xylyl)2]2- in dmf (dimethylformamide), dmso (dimethylsulphoxide) or a 1:1 dmf/dmso mixture, a model for the chromophore in the 2fe-2s proteins (ferredoxins), has been reduced and studied by conventional epr over a temperature range. the low-field feature of the spectrum, hz, has been computer simulated in order to analyse the lineshape in terms of a convolution product of lorentzian and gaussian distributions. the gaussian contribution to the linewidth and a fixed part of ... | 1983 | 6297591 |
| induction of suppressive cells within the t4 subset by a human t-t hybridoma-produced factor. | this study examines the target cell and the mode of action of a factor (tsf), produced by a human t-t hybridoma, which suppresses pokeweed mitogen-induced b cell differentiation as previously reported (j. immunol. 1982. 129: 1008.). the suppressive effect still exists when peripheral blood leukocytes are depleted of t8+ cells or when tsf is added to a mixture of purified t4+ and b cells. this suppressive effect disappears when the t4 cells are irradiated. the suppression, however, is restored wh ... | 1983 | 6229406 |
| functional differences of anti-t-cell antibody in patients with systemic lupus erythematosus and ulcerative colitis. | the loss of suppressor t-cell function results in an abundant production of autoantibodies in systemic lupus erythematosus (sle). as a cause of this suppressor t-cell defect, anti-t-cell antibody seems to be of prime importance. on the other hand, anti-t-cell antibodies can be detected in various other autoimmune diseases, but their functional characteristics have not been determined. in the present study, the functional characteristics of anti-t-cell antibody from a selected subgroup of patient ... | 1983 | 6229872 |
| persistent generalized lymphadenopathy in homosexual men: clinical, pathological and immunologic characteristics. | eighteen homosexual men who had had lymphadenopathy in two or more extrainguinal sites for more than 5 months but had no evidence of other illnesses or infections were studied. all had extreme malaise, and 90% had additional symptoms (fever, night sweats, weight loss or gastrointestinal dysfunction). they were compared with 10 healthy homosexual and 10 healthy heterosexual male controls. the mean numbers of circulating t8 (suppressor) lymphocytes were increased equally in the two homosexual grou ... | 1983 | 6231088 |
| biological functions of t-cell surface glycoproteins. | the okt4 monoclonal antibody reacts with a 62 kd cell surface glycoprotein present on a subset of human t cells with the capacity to help or induce b-cell differentiation. the okt8 monoclonal antibody reacts with a 76 kd cell surface glycoprotein present on a subset of human t cells with the capacity to suppress b-cell differentiation. the current studies were undertaken to determine whether the t4 and/or t8 antigens themselves play any role in the helper or suppressor function mediated by okt4+ ... | 1983 | 6238748 |
| eluted rheumatoid synovial tissue t cell subsets and hla-dr bearing cells at different stages of fractionation. | by using monoclonal antibodies of the ok series in the indirect immunofluorescence technique we wanted to enumerate t cell subsets and hla-dr-bearing cells of patients with classical rheumatoid arthritis after the cells were eluted from rheumatoid synovial tissue and at different stages of two cell fractionation procedures. the procedures were an overnight incubation on plastic flasks or a brief (7 min) fractionation on nylon wool columns, both followed by isopaque-ficoll gradient centrifugation ... | 1983 | 6236015 |
| [changes in serum levels of t4, t3 and tsh in trh stimulation test among normal adults]. | 1983 | 6234078 | |
| responder cells in the human autologous mixed lymphocyte reaction (amlr). characterization and interactions in healthy individuals and patients with systemic lupus erythematosus. | in the course of a primary amlr, purified t4 cells, but not purified t8 cells, proliferate to the non-t cell stimuli; however, in the presence of t4 cells or helper factors secreted by t4 cells, also t8 lymphocytes are able to proliferate in the amlr. these observations together with findings on immunoregulatory capacities of amlr-activated t cells suggest that the amlr represents a model inducer circuit. in patients with sle, our preliminary data indicate a deficient response of t4 cells upon s ... | 1983 | 6242332 |
| a cloned, antigen-specific, ia-restricted lyt-1+,2- t cell with suppressive activity. | the correlation between cell surface antigen phenotype and function is one of the cornerstones of modern cellular and clinical immunology. it is based on the collective experience of many investigators examining populations of t cells. the availability of cloned lines of t cells now allows us to ask whether all cells sharing cell surface antigen phenotype are functionally equivalent. we have examined a large number of antigen-specific, self-ia recognizing, cloned lyt-1+,2- t cell lines for their ... | 1983 | 6242849 |
| correlation between helper/suppressor ratio and iga levels in the peripheral blood from patients with liver diseases. | the correlation between serum immunoglobulin levels and "helper/suppressor" (t4/t8) ratio has been investigated in 45 patients with biopsy proven liver diseases (22 with ald, 16 with cah and 7 with vhr). in the ald and cah groups the t4/t8 ratio was significantly higher than in normal controls and a strong correlation was noticed with iga, but not igg and igm levels. the same correlation was found in the vhr group as well, even if the mean value for the t4/t8 ratio in these patients was not sign ... | 1983 | 6229288 |
| lymphocyte subsets studied with monoclonal antibodies in liver tissues of patients with alcoholic liver disease. | in order to determine the nature of t lymphocytes accumulating in hepatic tissues of patients with alcoholic liver disease, we phenotyped these cells in situ, using monoclonal antibodies to lymphocyte surface antigens and the avidin-biotin-peroxidase complex (abc) technique. in portal areas of cirrhotic livers, a significant increase (p less than 0.001) in t lymphocytes was observed as compared to livers showing only fatty changes and normal liver tissues. the t cells accumulating in cirrhotic l ... | 1983 | 6229193 |
| [relapse in basedow's disease after treatment with synthetic antithyroid drugs. prognostic value of analysis of the hla system]. | one hundred and eleven unselected patients with hyperthyroidism due to graves' disease received decreasing doses of carbimazole for 18 months. clinical examination and hormonal assays (serum t3, t4, free t4 index) were done at 4, 9 and 18 months of treatment. patients were typed for 35 hla antigens and were followed up for 2 years after withdrawal of treatment; 39 patients were excluded for various reasons and 72 were retained for study. of the 72 patients, 37 relapsed and 35 remained in remissi ... | 1983 | 6228849 |
| do anti-t4 (leu 3) and anti-t8 (leu 2) monoclonal antibodies delineate t-cell subsets with helper/inducer and suppressor/cytotoxic function, respectively? | 1983 | 6229066 | |
| depletion of lymphocytes with membrane markers of helper phenotype: a feature of acute and chronic drug-induced immunosuppression. | t cell subsets tested with markers for fc receptors for ig (tm, tg and eahu rosettes) or monoclonal antibodies (t4 and t8 lymphocytes) were investigated both in normal volunteers and in kidney transplant recipients with a well functioning graft and receiving low immunosuppressive therapy, before and 4 hr after administration of 100 mg of hydrocortisone. hydrocortisone induced a redistribution which was characterized by a decrease in the percentages of tm (38 +/- 2.4 before; 22 +/- 2.9 after; p l ... | 1983 | 6220849 |
| acquired immunodeficiency syndrome in a colony of macaque monkeys. | a naturally occurring immunodeficiency syndrome has been seen in a captive colony of macaque monkeys. this syndrome is seen primarily in the species macaca cyclopis. affected animals died with lymphomas (a rare disease in macaques) or such opportunistic infections as pneumocystis carinii and noma (necrotizing gingivitis). these m. cyclopis exhibited anemia, neutropenia, and a circulating bizarre immature monocyte. in addition, liver function tests suggested hepatitis. pokeweed mitogen-, concanav ... | 1983 | 6221343 |
| a physiological dose of triiodothyronine normalizes cardiac myosin adenosine triphosphatase activity and changes myosin isoenzyme distribution in semistarved rats. | the possibility that the lowering of thyroid hormone levels which occurs in the nonthyroidal illness syndrome results in a hypothyroid state at the cardiac tissue level was examined in semistarved rats. rats were fed 50% of their normal food intake in the form of a regular diet (r. diet) or low carbohydrate diet (l.c. diet) for 8 weeks. animals semistarved for 8 weeks on the r. diet lost 42% of their body weight, while plasma t3 and t4 levels decreased by 45-50%. semistarvation on the l.c. diet ... | 1983 | 6221921 |
| antigen-binding human t suppressor cells and their association with the hla-dr locus. | the ability of human lymphocytes to bind antigen was studied by direct binding of 125i-labeled streptococcal protein antigen, followed by autoradiography. t-enriched lymphocytes depleted of adherent cells and b cells showed specific binding of 125i-labeled streptococcal antigen (sa) at 4 degrees c and in the presence of sodium azide. further depletions of the t-enriched population by the monoclonal t4 or t8 antiserum and complement revealed that the antigen-binding t cell is t4-, t8+. this was c ... | 1983 | 6221933 |
| multiple sclerosis. distribution of t cells, t cell subsets and ia-positive macrophages in lesions of different ages. | using monoclonal antibodies in combination with the pap technique, total (t11+) t cells, helper-inducer (t4+) t cells, suppressor-cytotoxic (t8+) t cells and ia+ cells (macrophages and b cells) were localized in frozen sections of multiple sclerosis (ms) lesions with varied disease activity. in acute ms, t11+, t4+, t8+ cells and ia+ macrophages were found in large numbers throughout the lesion but were virtually absent from normal white matter. in active chronic ms lesions, the numbers of t11+, ... | 1983 | 6222066 |
| contribution of 3' leads to 5' exonuclease activity of dna polymerase iii holoenzyme from escherichia coli to specificity. | the effects of deoxynucleoside monophosphates on the 3' leads to 5' exonuclease activity of dna polymerase iii holoenzyme have been correlated with their effects on the fidelity of dna replication. in particular, dgmp inhibits the proofreading activity of the enzyme and decreases the fidelity in those cases where a "following nucleotide effect" is also noted. this is strong evidence for proofreading. however, the absence of the effects of proofreading inhibitors or following nucleotides need not ... | 1983 | 6222198 |
| peripheral blood lymphocyte subpopulations in polycythaemia and thrombocythaemia. | lymphocyte subpopulations from peripheral blood of normal subjects and patients with primary proliferative polycythaemia (ppp), idiopathic erythrocytosis (ie) and essential thrombocythaemia (et) were separated using antihuman immunoglobulin antiserum for b lymphocytes and the following monoclonal antibodies: okt3, directed against the general t-lymphocyte subpopulation, okt4 and okt8, detecting respectively t-helper and t-suppressor lymphocyte subpopulations, okm1 reacting mainly with monocytes. ... | 1983 | 6222468 |
| suppressive effect of human natural killer cells on pokeweed mitogen-induced b cell differentiation. | the suppressive effect of human natural killer (nk) cells on b cell differentiation induced by pokeweed mitogen (pwm) was investigated. by using percoll discontinuous density gradient centrifugation, peripheral blood nonphagocytic and nonadherent mononuclear cells were divided into low and high density fractions for which nk cells (large granular lymphocytes, lgl) and t cells were enriched, respectively. these fractionated mononuclear cells were co-cultured with purified autologous b cells in th ... | 1983 | 6223088 |
| modulation of surface antigens of a human monocyte cell line, u937, during incubation with t lymphocyte-conditioned medium: detection of t4 antigen and its presence on normal blood monocytes. | the human monocyte line, u937, derived from an individual with histiocytic lymphoma, undergoes morphological and functional changes when incubated with medium conditioned by lectin-stimulated cloned human t lymphocytes. using monoclonal antibodies and flow cytometry, we therefore analyzed alterations in surface components that might accompany these morphological changes, in comparison with components present on normal blood monocytes. the u937 cells possess three surface antigens in common with ... | 1983 | 6223092 |
| generalized lymphadenopathy and t cell abnormalities in hemophilia a. | two patients with hemophilia a had generalized lymphadenopathy, lymphopenia, elevated igg values, depressed t4 (helper) lymphocytes, elevated t8 (suppressor) lymphocytes, and abnormally low t4/t8 ratios. one of the patients, who also had hepatosplenomegaly, underwent cervical lymph node biopsy; the node contained 43% t8-lymphocytes, a marked elevation over the small fraction of t8 cells usually found in lymph nodes. these patients may have a form of the acquired immune deficiency syndrome descri ... | 1983 | 6223133 |
| selective effects of alpha interferon on human t-lymphocyte subsets during mixed lymphocyte cultures. | mixed lymphocyte reaction (mlr) cultures of human lymphocyte subsets with or without the addition of physiological doses of human alpha interferon (ifn-alpha) were compared with respect to surface marker phenotypes and proliferative capacities of the responder cells. a selective depression on the t4 (inducer) t-cell subset could be demonstrated as a sequence of events: decreased fluorescence intensity of the t4 inducer cells (day 2 of culture), decreased percentages of t4 cells as demonstrated b ... | 1983 | 6223362 |
| [a selective loss of t4 cells responding to autologous non-t cells in some patients with behçet's disease]. | 1983 | 6223384 | |
| heterogeneity of immunoregulatory t cells in human thyroid autoimmunity: influence of thyroid status. | monoclonal antibodies of the okt series were used to identify circulating t lymphocytes (okt3+), their helper-inducer (okt4+) and suppressor-cytotoxic (okt8+) subsets and cells bearing ia antigen (okia+) in 75 patients with thyroid autoimmune disorders, including 14 graves' disease, 21 myxoedema, 20 asymptomatic thyroiditis, 12 hashimoto's thyroiditis and eight simple goitre with superimposed thyroiditis. in the whole population of patients, a negative correlation was observed between the percen ... | 1983 | 6223729 |
| long-lasting impairment of immune and endocrine systems of offspring induced by injection of dexamethasone into pregnant mice. | dexamethasone was injected daily (2.5 and 5 micrograms/day) into pregnant mice from the 8th gestational day until delivery, and their offspring were examined in terms of body weight, organ histopathology, immunological function, and serum hormone level. two different postnatal patterns were observed among the treated offspring: one pattern showing a wasting appearance and death within 1 week, and the other showing retardation of growth without any wasting appearance. in the latter, the offspring ... | 1983 | 6223748 |
| [high dose medroxyprogesteroneacetate in metastasizing breast cancer: correlations between course of the disease and hormone profiles]. | based upon preliminary observations that tumor response to mpa was correlated to cortisol suppression 42 patients were treated with mpa at different dose levels. 1500 mg mpa p.o. almost completely suppressed endogenous cortisol production in 23 out of 23 patients. consequently, 51 patients with advanced stage metastatic breast cancer were treated with medroxyprogesteroneacetate (hd-map) at a dosage of 1500 mg p.o. daily or 500 mg i.m. on 5 days per week. there were 5 complete and 7 partical remi ... | 1983 | 6224046 |
| t8 chronic lymphocytic leukemia. a distinctive disorder related to t8 lymphocytosis. | a case of t chronic lymphocytic leukemia (cll) was first suspected on histologic and cytochemical examination of a splenectomy specimen. later the patient became frankly leukemic with an e+/t3+/t8+/t4- phenotype. from our case and other reported cases of t-cll studied with monoclonal antisera, the authors conclude that there are significant clinical and morphologic differences between t4 cll and t8 cll. in contrast to t4 cll, t8 cll characteristically presents with relatively low lymphocyte coun ... | 1983 | 6224415 |
| immunoregulation in glomerulonephritis, henoch--schonlein purpura and lupus nephritis. | immunoregulation was examined in normal controls and in patients with immune complex glomerulonephritis and lupus nephritis (sle) using okt monoclonal anti-bodies against helper (okt4) and suppressor (okt8) t cell subsets. functional studies assessed t cell control of in vitro immunoglobulin synthesis by cultured peripheral blood mononuclear cells (pbmc). igg and iga synthesis was measured in unstimulated, pokeweed mitogen (pwm) stimulated and pwm + concanavalin a (con a) stimulated cultures. pa ... | 1983 | 6224610 |
| epinephrine-induced changes in the distribution of lymphocyte subsets in peripheral blood of humans. | we have previously demonstrated that mitogen responsiveness of mononuclear cells (mnc) from peripheral blood is reduced after a single injection of epinephrine to human subjects. the purpose of the present study was to characterize the relative distributions of mnc subsets after epinephrine administration using monoclonal antibodies and conventional cell markers. the absolute number of circulating mnc increased 64% within 30 min after injection of epinephrine, and returned to baseline by 2 hr. a ... | 1983 | 6224849 |
| natural killer cell function and lymphocyte subpopulation profiles in inflammatory bowel disease. | peripheral blood natural killer (nk) cell activity and lymphocyte subpopulation profiles were studied in 23 patients with inflammatory bowel disease (ibd) and in 16 healthy control subjects. nk activity was assessed by a 4-hr 51cr release assay using k-562 erythroleukemia cell line targets. both monocyte depleted and nondepleted lymphocyte preparations were tested using 50:1 and 25:1 effector to target cell ratios. lymphocyte subpopulation profiles were determined by incubation of cell suspensio ... | 1983 | 6224936 |
| [antigenic markers of immunocompetent cells in rheumatoid synovial membrane]. | immunologic characteristics of cells present in rheumatoid synovium from 15 patients were analyzed either "in situ" on frozen sections or after elution. monoclonal antibodies directed against t cell subpopulation antigens and anti-ia-like were used. t cells bearing the phenotype t3+, t4+, ia+ are numerous, and they appeared gathered in lymphoid follicles and in perivascular areas. they are in close relationship with large ia+ cells probably macrophagic in nature. numerous dendritic cells as well ... | 1983 | 6225180 |
| [the thyroid and adrenal glands in rats chronically exposed to an intense electric field]. | two month-old male long-evans rats were exposed for 8 hours a day during 4 weeks to an ambient electric field (50 kv/m). the growth rate of electric field-exposed rats was identical to the controls' one. no significant effect of exposure was observed on the levels of various hormones: plasma and adrenal corticosterone; plasma tsh, acth, t3 and t4; adrenal epinephrine and norepinephrine. histological studies of thyroïd and adrenals showed a normal appearance. in adrenals, the levels of various co ... | 1983 | 6225485 |
| in vitro characterization of human intestinal intraepithelial lymphocytes. | enriched populations of human intestinal and colonic intraepithelial lymphocytes were isolated separate from lamina propria lymphocytes. these populations, and peripheral blood mononuclear cells, were characterized for membrane features, lymphocyte subsets, and proliferative potential to the nonspecific mitogenic lectins phytohemagglutinin, concanavalin a, and pokeweed mitogen. all three populations were predominantly t lymphocytes as measured by sheep red blood cell rosettes and monoclonal anti ... | 1983 | 6225690 |
| in situ characterization by monoclonal antibodies of the mononuclear cell infiltrate in chronic active hepatitis. | to characterize the inflammatory infiltrate of chronic active hepatitis we have examined 26 liver biopsy specimens [16 hepatitis b surface antigen-negative and 10 hepatitis b surface antigen-positive] with monoclonal antibodies and antiimmunoglobulins that react against the following mononuclear cell populations: t cells, t4 cells, t8 cells, monocytes-macrophages, ia-positive cells, surface ig-bearing cells, natural killer/killer cells. a biotin-avidin-fluorescein technique was used and the numb ... | 1983 | 6225691 |
| [t4 thyrotoxicosis: increase in the level of production of triiodothyronine (t3)]. | 1983 | 6226008 | |
| unexpected relationships between bacteriophage lambda hypothetical proteins and bacteriophage t4 tail-fiber proteins. | hypothetical lambda protein orf314 shows significant homology with the carboxyl end of phage t4 tail-fiber protein gp37. homology can also be demonstrated between hypothetical lambda protein orf194 and a fragment of bacteriophage t4 protein gp38. this sequence homology is also reflected in the genomic sequences of these two phages. | 1983 | 6226290 |
| immunoregulatory t cell subsets in chronic hepatitis b virus infection: the influence of homosexuality. | the purposes of this study were 2-fold: (i) to enumerate peripheral immunoregulatory t cell subsets in untreated patients with chronic hepatitis b virus (hbv) infection and (ii) to examine the relationship between disturbances in the balance of lymphocyte subsets with liver disease and the presence of homosexuality. circulating t lymphocyte subsets were evaluated by monoclonal antibodies to the following cell antigens: okt3 (total t cells), okt4 (helper/inducer t cells), and okt8 (suppressor/cyt ... | 1983 | 6226581 |
| relationship between human t cell functional heterogeneity and human t cell surface molecules. | our knowledge of human t cell differentiation and function has expotentially increased during recent years. with this growth in knowledge there has been an increase in our appreciation of the complexity of the t-t interactions which initiate and control immune responses. a great deal remains to be learned concerning the mechanisms of these complex cellular interactions. in particular, it will be important to precisely understand the clear heterogeneity of functions within isolated subsets of okt ... | 1983 | 6226584 |
| immunoregulatory t cell subpopulations in man: dissection by monoclonal antibodies and fc-receptors. | until now, most of the studies on regulatory t cells have been based on culture systems in which human peripheral blood cells are stimulated by polyclonal stimulators like pokeweed mitogen (pwm). our present contribution, however, deals with t cell-mediated regulation of the antigen-induced b cell activation, which exclusively leads to an antigen-specific igm production (heijnen et al. 1979a). some authors' reports on regulatory activities of t cells, as tested in systems using polyclonal stimul ... | 1983 | 6226587 |
| stimulation by thyroid hormone analogues of red blood cell ca2+-atpase activity in vitro. correlations between hormone structure and biological activity in a human cell system. | human red blood cell membrane ca2+-atpase activity is stimulated in vitro by physiological concentrations (10(-10) m) of l-thyroxine (l-t4) and 3,5,3'-triiodo-l-thyronine (l-t3). this human cell system has been utilized to examine a series of iodothyronine and iodotyrosine analogues for structure-activity relationships. analogue purity was verified by high pressure liquid chromatography. analogues were studied at a concentration of 10(-10) m and the stimulatory effect of each analogue was compar ... | 1983 | 6226658 |
| impairment of the autologous mixed lymphocyte reaction in atopic dermatitis. | the t cell proliferative response to autologous non-t cells is termed the autologous mixed lymphocyte reaction (amlr). recent studies have suggested that the amlr represents an inducer circuit for the activation of t8+ suppressor/cytotoxic effector cells. since atopic dermatitis (ad) patients are deficient in t8+ cytolytic t cell function, we investigated the amlr in ad. when sheep erythrocytes were used to separate t cells from non-t cells, the amlr was found to be significantly decreased (p le ... | 1983 | 6226679 |
| characterization of human k cells by surface antigens and morphology at the single cell level. | human lymphocytes killing bovine erythrocytes in vitro in antibody-mediated reactions were characterized at the effector cell level in the adcc plaque assay. five to 10% of highly purified peripheral blood lymphocytes are active k cells in this system. forty to 50% of these were t gamma cells expressing the t cell-associated surface antigens t3 and leu-1. these cells also expressed the t8/leu-2a antigens (approximately 20%) or the t4/leu-3a antigens. although approximately 30% of the k cells wer ... | 1983 | 6226741 |
| the regulatory roles of t4 and t8 subsets in tetanus toxoid-induced in vitro immunoglobulin production. | a new mitogenic system for in vitro immunoglobulin production induced by tetanus toxoid is presented and the role of t4 and t8 subsets in tetanus toxoid-induced in vitro immunoglobulin production is investigated. purified t, t4, t8, and b cells from normal individuals previously immunized but not boosted with tetanus toxoid were cultured in helper and suppressor assays and the number of immunoglobulin-secreting cells were enumerated after culture using a hemolytic plaque assay. the regulatory ro ... | 1983 | 6227396 |
| lymphocyte subpopulations in the skin of patients with chronic urticaria. | in order to characterize the nature of the mononuclear cells in the perivascular infiltrates in the skin of 11 patients with cu, skin biopsy specimens were analyzed in situ by an avidin-biotin immunoperoxidase technique. serial frozen sections were stained for total t cells, helper-inducer t cells, suppressor-cytotoxic t cells, b cells, monocytes/macrophages, and hla-dr antigen. the infiltrates were found to consist mainly of t cells, whereas b cells and macrophages were rarely seen. most of the ... | 1983 | 6227650 |
| monoclonal antibody-defined peripheral blood t lymphocyte subpopulations in patients with mycosis fungoides. | a panel of monoclonal antibodies has been used to identify peripheral blood lymphocyte subpopulations in 13 patients with mycosis fungoides and in 13 normal subjects. an increase in the t6+ population and a decrease in the t8+ population were noticed in our patients. their t4/t8 ratio was considerably augmented. in 4 patients reevaluated after treatment, marked changes in the relative proportions of t lymphocyte subsets had occurred. the possible pathogenetic significance of these immunological ... | 1983 | 6227856 |
| t4 dna ligase can seal a nick in double-stranded dna limited by a 5'-phosphorylated base-free deoxyribose residue. | the 5' ap endodeoxyribonucleases hydrolyze the phosphodiester bond 5' to ap (apurinic or apyrimidinic) sites in double-stranded dna leaving 3'-oh and 5'-phosphate ends. these nicks are sealed by t4 dna ligase although the 5'-phosphate end belongs to a base-free deoxyribose. | 1983 | 6227866 |
| [kaposi's sarcoma and opportunistic infections in young patients without antecedents liable to involve immunodepression]. | the acquired immune deficiency syndrome (aids) is characterized by the occurrence, in a subject under 60, of kaposi's sarcoma and/or severe opportunistic infection due to deficiency of the normal cell-mediated immune defence mechanism against the causative agents. in a number of patients, aids is preceded by fever, weight loss, profuse sweating, polyadenopathy or diarrhoea. in an epidemiological study conducted by a french multidisciplinary group from march to december, 1982, 25 cases were colle ... | 1983 | 6227886 |
| studies on the t3 suppression test by the 24-hour thyroidal 131i uptake in patients with graves' disease: comparison of the effects of propylthiouracil and methylmercaptoimidazole. | the t3 suppression test by the 24-hr thyroidal 131i uptake was reevaluated in patients with graves' disease before and after withdrawal of antithyroid drug. fifty patients had been treated with propylthiouracil (ptu) or methylmercaptoimidazole (mmi) for 12 to 70 months. they were prescribed a maintenance dose of antithyroid drug (ptu, 50 mg/day; mmi, 5 mg/day) at the time of investigation and regarded as euthyroid on the basis of serum t3, t4 and tsh levels. each patient was given 75 micrograms ... | 1983 | 6200318 |
| direct demonstration of the human suppressor inducer subset by anti-t cell antibodies. | prior studies indicated that sera of patients with active juvenile rheumatoid arthritis (jra) contain anti-t cell antibodies reactive with the t4+ inducer population. more important, depletion of this t cell subset with jra anti-t cell antibodies (jra+ t cells) and c abrogated t5/t8+ suppressor t cell function. in the present study, we utilized ig-coated plate techniques and jra anti-t cell antibodies to fractionate the t4+ population into t4+jra+ and t4+jra- subsets and characterize the individ ... | 1983 | 6216285 |
| multiple sclerosis: distribution of t cell subsets within active chronic lesions. | the distribution of t cells and t cell subsets was examined within the human central nervous system in active lesions from seven patients with chronic multiple sclerosis. the monoclonal antibodies anti-t11, anti-t4, and anti-t8 were used to detect total (whole) t cells, helper t cells, and suppressor-cytotoxic t cells, respectively, and a monoclonal antibody against human ia was used for macrophages and b cells. lesion progression was associated with large numbers of t4+ cells at the lesion marg ... | 1983 | 6217550 |
| identification of t lymphocytes in ocular adnexal neoplasms by hybridoma monoclonal antibodies. | we performed indirect immunofluorescence with the okt series of hybridoma monoclonal antibodies in order to determine the total number of t cells and their subset distribution, that is, the percentages of helper (okt3+t4+t8-) and suppressor/cytotoxic (okt3+t4-t8+) t cells, in 28 ocular adnexal lymphoid neoplasms. okt3+t4+ (helper) t cells vastly predominated in each of ten benign, polyclonal ocular lymphoid proliferations. the helper-suppressor t-cell ratio (t4-8) ranged from 2.5 to 8.2 (mean, 5 ... | 1983 | 6218756 |
| [quantitative evaluation of hormonal extraction performed by continuous plasma exchange in euthyroid patients. application to the treatment of severe hyperthyroidism]. | plasma exchanges (pe) have been successfully used in the treatment of thyroid storm. the dramatic results obtained (as in the case reported here) have prompted the authors to evaluate the effects of pe on plasma thyroid hormone (t4, t3 and unbound t4) levels and thyroxine-binding globulin (tbg) levels in 5 euthyroid patients. pe of one blood volume consistently produced a significant decrease in t4, t3 and tbg levels without changes in unbound t4 or tsh. hormone extraction was proportional to th ... | 1983 | 6219358 |
| distinctive expression of the t4 antigen in normal and stimulated lymphocytes. | correlated light scatter and fluorescence flow cytometric analysis of human peripheral blood lymphocytes showed that the expression of the t4 antigen was higher in the larger lymphocytes than in the smaller lymphocytes. a similar expression pattern was observed for hla class i antigens but not for t3 and t8, whose expression was independent of cell size. results with lymphocytes from spleen, lymph node, and tonsil were comparable to those of peripheral blood. thymocytes, however, were smaller an ... | 1983 | 6219752 |
| human normal ctl clones: generation and properties. | this study reports the production of clones of human killer t cells grown in the presence of tcgf4 following sensitization in mlc against (1) allogeneic cells, (2) autologous bebv+ lymphocytes, or (3) autologous lymphoma cells. sensitization against the tumor cells required the addition of macrophages. the expression of the cytotoxic activity of the cloned t lymphocytes required re-stimulation with the specific stimulator cells. the cytotoxic activity seemed to be mhc-restricted, since (1) clone ... | 1983 | 6219963 |