Publications

rna splicing in prokaryotes: bacteriophage t4 leads the way.		1985	2580641
characterization of the human newborn response to herpesvirus antigen.	an investigation was made into the human newborn cellular response to herpes simplex virus type 1 (hsv), cytomegalovirus (cmv), and varicella zoster virus (vzv) to understand more about the nature of the neonate's susceptibility to overwhelming infection by these viruses. newborn mononuclear cells sustained the proliferation in culture of maternal (i.e., haplotype-matched) t cell blasts with specificity for hsv, cmv, or vzv (p less than 0.05). this is evidence for intact antigen-processing capab ...	1985	2580908
human t cell leukemia/lymphoma virus-infected antigen-specific t cell clones: indiscriminant helper function and lymphokine production.	the ability of human t cell leukemia/lymphoma virus (htlv)-i to alter the function of infected t lymphocytes was examined directly by investigating the properties of an antigen-specific t cell clone before and after transformation with htlv-i. following infection, the t4 antigen-specific clone manifested a tenfold increase in its surface interleukin 2 (il 2) receptor (tac) density and acquired the viral determinants p19, p24, and 4d12 not present in the uninfected clone. prior to infection, the ...	1985	2580910
changes in cardiac function following administration of thyroid hormones in thyroidectomised rats: assessment using the isolated working rat heart preparation.	we investigated the changes in cardiac function following alterations in thyroid state of rats, using the isolated working heart preparation. this technique allows heart rate, contractility, and pump function to be studied under controlled conditions. surgical thyroidectomy resulted in significant impairment of cardiac function. single doses of 3,5,3'-triiodo-l-thyronine (t3) or l-thyroxine (t4) were given to thyroidectomised rats. cardiac function was restored to normal within 48 h of administr ...	1985	2581083
combination chemotherapy with high-dose methotrexate, bleomycin, and cisplatin in management of head and neck squamous cell carcinoma.	fifty-nine patients with stage iv head and neck squamous cell cancer were treated with an intensive induction chemotherapy consisting of high-dose methotrexate-leucovorin, bleomycin, and cisplatin. forty-five patients had recurrent disease following surgery and/or radiation therapy. the response rate in this group was 22%, with a median response duration of 10 weeks and a median survival of 19 weeks. the median survival in responders was 20 weeks and in nonresponders 18 weeks. fourteen previousl ...	1985	2581436
effects of antithyroid drug therapy on blood glucose, serum insulin, and insulin binding to red blood cells in hyperthyroid patients of different ages.	the mechanism of glucose intolerance in thyrotoxicosis was investigated in 119 patients with graves's disease with careful consideration of the age-related deterioration of glucose tolerance. before and after treatment of thyrotoxicosis with antithyroid drug, changes of blood glucose (bg) and serum immunoreactive insulin (iri) in response to 50 g oral glucose tolerance test (ogtt) and insulin binding to red blood cell (rbc) were evaluated. in control subjects, the sigma iri/sigma bg ratio after ...	1985	2581747
properties of a dna repair endonuclease from mouse plasmacytoma cells.	the properties of a dna-repair endonuclease isolated from mouse plasmacytoma cells have been further studied. it acted on ultraviolet-light-irradiated supercoiled dna, and the requirement for a supercoiled substrate was absolute at ultraviolet light doses below 1.5 kj m-2. at higher doses relaxed dna could also serve as a substrate, but the activity on this dna was due mostly to hydrolysis of ultraviolet-light-induced apurinic/apyrimidinic (ap) sites by the ap-endonuclease activity associated wi ...	1985	2581776
polyadenylated, noncapped rna from the archaebacterium methanococcus vannielii.	polyadenylated [poly(a)+] rna molecules have been isolated from methanococcus vannielii by oligodeoxythymidylate-cellulose affinity chromatography at 4 degrees c. approximately 16% of the label in rna isolated from cultures allowed to incorporate [3h]uridine for 3 min at 37 degrees c was poly(a)+ rna. in contrast, less than 1% of the radioactivity in rna labeled over a period of several generations was contained in poly(a)+ rna molecules. electrophoretic separation of poly(a)+ rna molecules show ...	1985	2581934
dna primase-dna polymerase alpha from simian cells. modulation of rna primer synthesis by ribonucleoside triphosphates.	dna primase-dna polymerase alpha, purified 53,000-fold from cv-1 cells, synthesized predominantly (p)ppa(pa)6-primed dna on a poly(dt) template. about 80% of the rna primers synthesized on an m13 dna template were (p)ppa/g(pn)5-7, and 20% were (p)ppa/g(pn)0-4. rna primer size was determined by gel electrophoresis after removing nascent dna with phage t4 dna polymerase 3'-5' exonuclease, leaving a single dnmp at the 3'-end of the rna primer, and the terminal 5'-(p)ppn residue was determined by "c ...	1985	2581949
t3 monoclonal antibody activation of nonspecific cytolysis: a mechanism of ctl inhibition.	the t3 antigen is expressed on all cytotoxic t lymphocytes (ctl). monoclonal antibodies (mab) to the t3 antigen previously have been shown to inhibit ctl-mediated killing of cells expressing the relevant target antigens. the mechanism of t3 mab inhibition, however, remains undefined. in this report, we describe a novel effect of the t3 mab: the stimulation of allospecific ctl clones to kill target cells that do not express the relevant hla antigens. the stimulation of nonspecific killing was see ...	1985	2582036
systemic lupus erythematosus with deficiency of the t4 epitope on t helper/inducer cells.	three black jamaicans with systemic lupus erythematosus (sle) were identified whose t helper/inducer cells lacked the t4 epitope (t4 epitope-deficient phenotype). all three patients had lymphadenopathy as part of their syndromes. the asymptomatic and otherwise healthy t4 epitope-deficient brother of one of these patients also had lymphadenopathy in a distribution identical to that of his sister with sle. family studies pointed to an autosomal codominant mode of inheritance not linked to the hla ...	1985	2582253
chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.	biotin has been converted to 2-(biotinylamido)ethanol and condensed to phosphorylated oligonucleotides in a solid phase synthesis. the 5'-biotinylated oligonucleotides were enzymatically coupled to other dna fragments by t4 dna ligase or t4 rna ligase. the hybridization properties of such biotin-labeled oligonucleotide probes were studied.	1985	2582352
translesion dna synthesis: polymerase response to altered nucleotides.	a system for the determination of the specificity of incorporation opposite lesions during dna synthesis past damaged bases has been used as a model of mutation. the system, based on the dideoxynucleotide sequence method, uses lesions in the template strand as chain terminators. as a first approximation, such lesions constitute non-instructive sites in the dna. dna synthesis catalysed by bacteriophage t4 dna polymerase terminates one nucleotide 3' to the lesion on the template strand. with other ...	1985	2825983
serum thyroid hormone levels in male buffalo calves as related to age and sexual development.	a total of 155 male buffalo calves were classified into 17 groups according to chronological age. the body weight was recorded on a balance or computed. sera samples were analysed for thyroxine (t(4)) and triiodothyronine (t(3)) by radioimmunoassay. highest concentration of thyroid hormones (t(4), 87.6+/-17.6; t(3), 3.1+/-0.07 ng/ml) were seen during the first week after birth followed by a gradual decline until two months of age. later, the mean t(4) and t(3) levels fluctuated between 30 and 40 ...	1985	16726106
pituitary-thyroid hormone economy in healthy aging men: basal indices of thyroid function and thyrotropin responses to constant infusions of thyrotropin releasing hormone.	in order to assess the effects of aging, as distinct from those of thyroid disease or extrathyroidal illness, on certain indices of thyroid function, we studied 74 healthy, ambulatory men recruited from the baltimore longitudinal study on aging. we determined basal serum values of t4, t3, rt3, thyroxine-binding globulin (tbg), and t3 resin uptake (t3ru) and calculated the free t4 index (ft4i = t4 x t3ru/100), free t3 index (ft3i = t3 x t3ru/100), and t4/tbg ratio for each subject. we used an ult ...	1984	6420436
evidence for dopaminergic stimulation of growth velocity in some hypopituitary children.	the purpose of this study was to determine if endogenous hgh release, hence growth, in hypopituitary children could be potentiated by therapy with dopaminergic (da) drugs namely, l-dopa or bromocriptine. the effect of da therapy on other endocrine function was also examined. subjects were nine prepubertal children (four girls and five boys) with bone ages (ba) ranging from 1.5-9.5 yr. they were diagnosed as having idiopathic gh deficiency on the basis of: 1) failure to grow at normal rates 2) la ...	1984	6420440
delineation of lymphocyte subsets in lymph nodes of nonhuman primates.	monoclonal antibodies which recognize specific surface antigens on human lymphocytes were used with an avidin-biotin immunoperoxidase technique to stain lymph nodes from nonhuman primates. cells in lymph nodes from three macaque species and two new-world primate species could be stained to define t11, t4, t8, and b1 lymphocyte subsets. this approach will facilitate the study of many important spontaneous and experimentally induced diseases of nonhuman primates.	1984	6421525
changes in membrane-associated proteins during sporulation in bacillus subtilis.	membrane proteins from vegetative and sporulating cells of bacillus subtilis were separated by the two-dimensional gel electrophoresis system using isoelectric focusing and sodium dodecyl sulfate/polyacrylamide gel electrophoresis (o'farrell technique). membrane proteins were isolated according to published procedures. the gels were stained with coomassie blue. three different concentrations of proteins were analyzed to detect even minor constituents. over two hundred different membrane proteins ...	1984	6421583
plasma thyroxine levels in freshwater perch: influence of season, gonadotropins, and gonadal hormones.	plasma thyroxine (t4) of a freshwater female perch anabas testudineus (bloch) has been measured by specific radioimmunoassay (ria). monitoring of plasma t4 throughout the year showed that july to early september (spawning stage) had the highest t4 and late september to january (postspawning stage) had the lowest t4. the plasma t4 cycle was coincident with the ovarian cycle. treatment of intact perch with salmon gonadotropin (sg-g100) or ovine lh (500 ng/100 g body wt) markedly enhanced plasma t4 ...	1984	6421653
tsh response to trh in hemodialysis and capd patients.	the thyroid status was studied in 19 uremic patients (11 on hd and 8 on capd) and in a group of 17 healthy adult patients. in uremic patients t3 and t4 were significantly reduced (p less than 0.001) and rt3 was increased (p less than 0.001). the time course tsh response to trh showed a lower peak response (in 5 patients it did not exceed 5 microu/ml) which on turn was also delayed (the peak was observed at 60 minutes in 63% of them). tsh concentrations in uremics were increased at 0 time (p less ...	1984	6421756
involvement of envelope-bound calcium in the transient depolarization of the escherichia coli cytoplasmic membrane induced by bacteriophage t4 and t5 adsorption.	we previously showed that adsorption of bacteriophages t4 and t5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. as these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal which must be transmitted between the two membranes. we show here that these two phages use different mechanisms of transmission of this stimulation signal. in the case of t4, but not o ...	1984	6421800
a competitive ligand binding assay for measurement of thyroid hormone-binding inhibitor in serum and tissues.	a competitive ligand-binding assay (clba) is described for measurement of an inhibitor(s) of serum binding of t4 in ether extracts of serum and in homogenates and extracts of tissues. the clba is based on the effect of thyroid hormone binding inhibitor (thbi) on partition of a constant amount of radiolabeled ligand [(125i]t4) between fixed amounts of serum and an anti-t4 antibody. the method is convenient, rapid, sensitive, and reproducible. the coefficient of variation averaged 8.9% within an a ...	1984	6421865
reappraisal of the 3,5,3'-triiodothyronine-suppression test in the prediction of long term outcome of antithyroid drug therapy in patients with hyperthyroid graves' disease.	thyroidal suppressibility by exogenous t3 in terms of both radioiodine uptake (raiu) and serum t4 was evaluated in 115 hyperthyroid patients treated with methimazole for 2 yr and followed for an additional 2 yr to study the rate of recurrence. various other serum parameters including serum thyroglobulin concentrations, thyroid autoantibody, and tsh receptor antibody titers, and thyroidal responses to trh-induced tsh elevation were also determined. after 2 yr of methimazole therapy, thyroidal rai ...	1984	6421866
clonal heterogeneity in the requirement for t3, t4, and t8 molecules in human cytolytic t lymphocyte function.	in an attempt to define the requirement of t8, t4, and t3 surface molecules in functional interactions occurring between human cytolytic t lymphocytes (ctl) and specific target cells, we have analyzed a large number of ctl clones derived from primary mixed lymphocyte culture (mlc) t cell populations for their susceptibility to inhibition by monoclonal antibodies (mab) directed against these surface antigens. in most experiments, mlc t cells were stained with b9.4 (anti-t8) or okt4 (anti-t4) mab, ...	1984	6421984
isolation of the prohead core of bacteriophage t4 after cross-linking and determination of protein composition.	the naked core of bacteriophage t4 was isolated ex vivo after cross-linking with either glutaraldehyde or dithiobis(succinimidyl propionate). the isolated particles appeared to be morphologically identical to the cores found in thin sections, to those demonstrated in in situ lysis preparations, and to core structures assembled in vitro. treatment with glutaraldehyde provided core particles which were morphologically well preserved, whereas dithiobis(succinimidyl propionate)-induced cross-linking ...	1984	6422052
screening for congenital hypothyroidism: a three year experience.	experience with a nationwide screening programme for congenital hypothyroidism in new zealand is reported. using radio-immune assays for thyroxine (t4) on all newborns, and thyroid stimulating hormone (tsh) determinations on samples with t4 levels below the 5th percentile, 160 899 babies were screened and 33 cases of primary hypothyroidism were detected (incidence 1: 4875). screening resulted in treatment beginning within the first three weeks of life.	1984	6422361
modulation of human t cell production of migration inhibitory lymphokines by cytokines derived from human leukocyte dialysates.	human leukocyte dialysates contain components capable of amplifying cutaneous delayed-type hypersensitivity (dth) reactions. in the present study, two such amplifiers, both less than 3500 m.w., were partially purified from human leukocyte dialysates by gel filtration on sephadex g-10 followed by high pressure reverse-phase liquid chromatography. these amplifiers of dth were examined for their effects on production of the migration inhibitory lymphokines leukocyte migration inhibition factor (lif ...	1984	6361122
the dosage dependency of growth and maturity in growth hormone deficiency treated with human growth hormone.	a group of 11 pre-pubertal growth hormone deficient patients were treated with human growth hormone over a period of 4 years. in 6 of the patients the dosage was 4 iu 3 times a week and in 5, 8 iu 3 times a week. changes in height demonstrated that the "catch up" was significantly greater and of longer duration in the second group. in spite of a more rapid increase of bone age in the second group, the prognosis of final height had improved significantly at the end of the study period. a comparat ...	1984	6422697
treatment experience with 15 mev fast neutrons in the oral cavity and oropharynx.	all 86 patients with squamous cell carcinoma of the oral cavity and oropharynx treated with fast neutrons at the mid-atlantic neutron therapy facility at the naval research laboratory (manta) from its inception in 1976 until closing in 1979, are reported. patients generally had advanced disease or have failed or were failing conventional treatment prior to being treated at manta. the fixed horizontal beam parameters were suboptimal. patients were treated by either neutrons alone or various combi ...	1984	6423262
completion of excision repair patches in human cell preparations: identification of a probable mode of excision and resynthesis.	excision repair of u.v. damage in human fibroblasts is more sensitive to inhibitors of dna polymerase alpha (cytosine arabinoside, aphidicolin) than to an inhibitor of polymerase beta (dideoxythymidine), which indicates a greater role in repair for polymerase alpha than for polymerase beta. these inhibitors all generate shortened patches with free 3' termini; the detailed structure of these patches was investigated in permeable cells or isolated nuclei by degradation of dna with exonuclease iii ...	1984	6423305
responses to thyrotropin during development in japanese quail.	japanese quail, a species with a precocial pattern of development, were used as a model for studying the ontogeny of thyroid responses to tsh. thiourea was administered to embryos early in incubation, and the effects were assessed by measuring thyroid 32p uptake, serum thyroid hormones, thyroid weights, and body weights. thyroid stimulation after thiourea treatment indicated that maturation of thyroid-pituitary negative feedback occurs between days 9 and 10 of the 16.5-day incubation period. sin ...	1984	6423370
thyrotropin-releasing hormone stimulates growth hormone release from the anterior pituitary of hypothyroid rats in vitro.	we have examined the interaction of thyroid hormone and trh on gh release from rat pituitary monolayer cultures and perifused rat pituitary fragments. trh (10(-9) and 10(-8)m) consistently stimulated the release of tsh and prl, but not gh, in pituitary cell cultures of euthyroid male rats. basal and trh-stimulated tsh secretion were significantly increased in cells from thyroidectomized rats cultured in medium supplemented with hypothyroid serum, and a dose-related stimulation of gh release by 1 ...	1984	6423373
effect of amiodarone on thyroid hormone economy.	serum thyroxine (t4), triiodothyronine (t3), resin uptake of t3 (rt3u), thyroid stimulating hormone (tsh) and tsh response to thyrotropin releasing hormone (trh) were measured in 92 patients treated with amiodarone for up to 4 years. two patients developed thyrotoxicosis, while euthyroid hyperthyroxinemia occurred in 29 (32%). hypothyroidism was diagnosed in 11 patients (12%), and a further 11 had tests consistent with a "failing thyroid." of 39 patients with normal values of t4, 15 had abnormal ...	1984	6423565
effects of hysterectomy and uterine extracts on the endocrine system of adult, nonpregnant rats.	plasma concentration and anterior pituitary content of growth hormone (rgh), thyroid stimulating hormone (rtsh), prolactin, follicle stimulating hormone (rfsh), luteinizing hormone (rlh) as well as the plasma concentrations of triiodothyronine (t3), thyroxine (t4), corticosterone and immunoreactive somatomedin a (sm-a) have been determined in intact, ovariectomized or ovo-hysterectomized rats with and without treatment with steroid-free uterine extracts. the endocrine organs were weighed. two we ...	1984	6423899
[calcitonin and parathyroid hormone secretion and calcium metabolism in patients with diffuse toxic goiter during treatment with lithium carbonate].	seventy-six patients (6 males and 70 females) with diffuse toxic goiter, stages i-ii, received lithium carbonate as a thyrostatic drug. the drug dose ranged from 900 to 1500 g depending on the degree of the disease clinical symptoms. the treatment with lithium lasted 45 days. before drug administration and on days 7, 15, 30 and 45 of treatment the content of triiodothyronine (t3), thyroxine (t4) and calcitonin was measured in the thyroid, that of parathyroid hormone (pth) in the parathyroid glan ...	1984	6424108
thyroxine and thyroxine-binding globulin: discriminant formulae for the separation of the diagnostic classes of thyroid function.	the biochemical classification of thyroid functional status is often accomplished by the use of the thyroxine to thyroxine-binding globulin (t4:tbg) ratio. it has recently been shown, however, that the reference range of the t4:tbg ratio varies with the concentration of tbg. this makes the t4:tbg ratio an unwieldy diagnostic index. a more accurate and economical way to define thyroid function using t4 and tbg measurements is by linear discriminant formulae. this is so because t4 varies linearly ...	1984	6424542
effects of dextro-thyroxine, a preparation almost free of levo-thyroxine, on thyroid function, serum lipids and apolipoprotein a-i in "double pre-beta lipoproteinemia".	eight subjects, belonging to a large family kindred repeatedly showing the electrophoretic pattern of the "double pre-beta lipoproteinemia", were studied. in seven of them thyroid function, serum lipids and apolipoprotein a-i were determined before and after treatment with dextro-thyroxine, preparation almost free of levo-thyroxine. in most of the patients, total-t4 levels and free-t4 index were in the lower normal range, but basal tsh levels and the tsh response to trh were normal. dextro-thyro ...	1984	6424687
[thyrotrophin releasing hormone (trh) stimulates the secretion of t4 by perfused thyroid fragments from rats].	evidence of a direct stimulating effect of trh on t4 secretion was found by using the perifusion technique on rat thyroid fragments. a dose-response curve was plotted and the kinetic pattern of the response was studied.	1984	6424894
interrelationships among body mass, thyrotropin, thyroid hormones, and thyroid-hormone binding proteins in healthy 70-year-old men.	in a previous study of a representative population of 460 men of age 70, we found that the free thyroxin (free t4) concentration was negatively correlated to body mass. in the present study we analyzed other indicators of thyroid function in relation to body mass and body mass index in 181 individuals without any disease or medication influencing thyroidal homeostasis or body mass. there was a significant negative relationship between body mass and body mass index and free t4, total t4, and 3,3' ...	1984	6424961
discrepancies between serum free triiodothyronine and free thyroxin as measured by equilibrium dialysis and analog radioimmunoassay in nonthyroidal illnesses.	we determined free triiodothyronine (ft3) and free thyroxin (ft4) in serum of patients with various nonthyroidal illnesses (nti), by both commercial analog-type radioimmunoassays (a) and new equilibrium dialysis procedures (d). the mean ft3 was significantly lower in nti by both techniques, but the decreases were of different magnitudes. the ft3(d):ft3(a) ratio was 1.96, vs 1.11 for healthy controls. in nti the mean ft4(d) was somewhat increased, whereas the mean ft4(a) was much lower than norma ...	1984	6424964
thyroid function in patients with homozygous beta-thalassaemia.	thyroid function was evaluated in 20 patients aged 8-30 years who were suffering from homozygous beta-thalassaemia. all patients have been receiving frequent blood transfusions and treated for the resulting transfusional iron overload with intramuscular injections of desferrioxamine. total thyroxine (t4), t3-uptake, total triiodothyronine (t3), and reverse triiodothyronine (rt3) were measured. in addition a standard trh stimulation test was performed and blood samples were checked for the presen ...	1984	6424976
prostaglandin i2 stimulates thyrotropin secretion in vivo and in vitro in rats.	effects of prostaglandin i2 (pg i2) on secretion of thyrotropin (tsh) in vivo and in vitro in rats have been studied. ten micrograms per kg of pg i2 were injected i. v. and the animals were serially decapitated. thyrotropin releasing hormone (trh), thyroxine (t4) and 3,3',5-triiodothyronine (t3) were determined by radioimmunoassay. effects of pg i2 from anterior pituitary were also investigated by means of an in vitro experiment. plasma and hypothalamic concentrations of immunoreactive (ir-trh) ...	1984	6425038
low serum thyroxine and high serum triiodothyronine in nephrotic rats: etiology and implications for bioavailability of protein-bound hormone.	traditionally, it has been thought that the bioavailable fraction of circulating serum hormones, i.e. that which is available for cellular uptake and is physiologically active, is limited to the free (nonprotein bound) hormone. however, recent evidence, based on acute organ uptake of labeled hormone, suggests that the amount of hormone which is bioavailable in vivo may exceed that which is calculated to be free in vitro. to explore the bioavailability of circulating protein-bound thyroid hormone ...	1984	6425040
thyroid hormones and carbohydrate metabolism of brain in the teleost, channa punctatus. i. effect of t4 and thiourea on succinic dehydrogenase (sdh) activity and protein content.	the brain of the fishes (channa punctatus) subjected to cold acclimation (15 +/- 1 degree) in darkness exhibited low succinic dehydrogenase (sdh) activity after 3 and 7 days and high protein content after 7 days when compared with their warm acclimated (32 +/- 1 degree) counterparts. in warm acclimated fishes maintained in complete darkness, t4 (0.5 micrograms/g body weight/day) depressed the enzyme activity after 5 and 7 days of treatment and reduced the protein content after 3 days. but neithe ...	1984	6425114
[function of the pituitary-thyroid axis after partial resection of the thyroid].	the tsh, t4 and t3 responses to trh were measured in 48 patients who underwent selective goitre resection (hemithyroidectomy, ablation of single nodule); moreover 3 groups of healthy, hypothyroid and hyperthyroid persons served as reference groups. the main object was to detect eventual onset of preclinical hypothyroidism. this condition was observed after hemithyroidectomy only in 12.5%, and never after ablation of single nodule. were detected too, among operated patients, 2 subjects with light ...	1984	6425739
antigenic stimulation regulates the level of expression of interleukin 2 receptor on human t cells.	antigen-specific, interleukin 2 (il-2)-dependent human t-cell lines and clones were utilized to study the relationship between il-2 receptor expression and antigenic stimulation. t cells that had not been exposed to antigen for 2 wk or more expressed a stable low level of the il-2 receptor. after reexposure to antigen, a 10- to 30-fold increase in the level of the il-2 receptor was rapidly induced, with the peak level of il-2 receptor expression occurring at 15-30 hr. this peak preceded the peak ...	1984	6425828
[free thyroxin as a determinant for thyroid function. evaluation of direct determination of ft4 compared to the oral trh test].	the diagnostic value of a direct assay of free thyroxine has been evaluated by comparing the concentration of free t4 with the results of an oral trh test. 147 patients were referred to an endocrine outpatient department because of suspected thyroid dysfunction. based on clinical investigation and on the results of an oral trh test, 75 patients were euthyroid, 12 hyperthyroid and 12 hypothyroid. preclinical hypothyroidism (3 h- tsh value above 35 me/l and euthyroid peripheral thyroid hormone lev ...	1984	6426055
[temporal dynamics of spectral eeg parameters during nocturnal sleep in healthy adults].	we recorded all-night sleep eeg's of six healthy male volunteers (age 23 to 29 years) from f3, f4, p3, p4, 01, 02, t3, t4 to cz as reference electrode. power and coherence spectra were calculated for ten frequency bands from 0-30 cps. we examined their changes through the different cycles of all sleep stages. in general there was a decrease in power as well as in coherence from the 1st to the 4th cycle of the different sleep stages with some variations depending on frequency and derivation. the ...	1984	6426930
red blood cell thyroxine in nonthyroid illness and in heparin-treated patients.	red blood cell t4 concentrations (rbc t4) were measured in 15 normal subjects, 13 patients with hypo- or hyperthyroidism, and 10 patients with elevated or decreased serum thyroid hormone binding. in each case, rbc t4 was compared with the serum concentration of free t4 measured by equilibrium dialysis ( ft4d ). rbc t4 correlated significantly with ft4d in these subjects (r = 0.90; p less than 0.001). the normal range for rbc t4 was 0.27-0.83 ng/ml. rbc t4 was below the normal range in all 8 pati ...	1984	6427259
reverse triiodothyronine does not alter pituitary-thyroid function in normal subjects.	serum rt3 concentrations are often increased in patients with nonthyroid illness. such elevations could be responsible for some of the alterations in pituitary-thyroid function that occur in such patients, particularly since rt3 is a potent inhibitor of extrathyroidal t3 production in vitro. to evaluate the role of serum rt3 elevations in the regulation of the hypothalamic-pituitary-thyroid axis, 10 normal subjects were given 3 mg rt3, orally, in divided doses for 4 days. serum rt3 concentration ...	1984	6427263
patterns of prolactin secretion in the syndrome of general resistance to thyroid hormones.	the dynamics of tsh and prl secretion were studied in three patients with incomplete generalized resistance to thyroid hormones. results in the first patient differed from those in the other two. the first patient had been treated previously with radioiodine but was clinically euthyroid when subsequently treated with supraphysiological quantities of t3 and t4. increasing doses of t4 from 0.2-0.3 mg/day caused a decline in serum tsh levels and lesser increments in tsh to injected trh in this pati ...	1984	6427264
evaluation of an immunoextraction procedure for the estimation of free thyroxine concentration.	we have examined the performance of a commercial free-thyroxine assay in which a radiolabeled t4 derivative permits the competitive quantitation of extracted t4 in the presence of serum proteins. after the total t4 pool had been radiolabeled with either i-125 t4 or i-131 t4, the solid-phase antibody was found to be associated with 4-8% of the total t4 present in the assay tube. of this, 15-60% was displaceable (antibody-bound). the assay estimated free t4 to be 0.6-1.8 ng/dl in euthyroid patient ...	1984	6427430
physiopathological aspects of body overweight in the female climacteric.	the correlation between overweight and the climacteric was studied in 550 menopause clinic patients by investigating certain clinical and sociocultural parameters (age, marital status, educational level, occupation and type of work, calorie intake, smoking habits, parity, blood pressure, previous hormonal therapy and climacteric symptoms), evaluating plasma levels of various hormones (17 beta-oestradiol, follicle-stimulating hormone (fsh), luteinizing (lh), testosterone, hydrocortisone, adrenoco ...	1984	6427551
transitory subclinical and permanent hypothyroidism in the course of subacute thyroiditis (de quervain).	sixty-two patients affected with subacute thyroiditis (sat) were followed for a mean period of 14 months (range 1-40), by monitoring thyroid hormone levels in basal condition, pituitary tsh reserve, antithyroglobulin (tgab) and antimicrosomal antibodies ( msab ), in order to study the natural course of the disease and to characterize its intermediate phase. in the first phase the mean serum iodothyronine levels were within normal limits, nevertheless elevated t3 and t4 levels were detected in 34 ...	1984	6428116
evidence of altered dopaminergic modulation of prl, lh and tsh secretion in patients with normoprolactinaemic amenorrhoea.	basal plasma concentrations of prl, lh, fsh, gh, tsh, t3, t4, resin t3 uptake ( rt3u ), and oestradiol as well as hormone responses to iv metoclopramide (mtc) were investigated in 16 consecutive patients with normoprolactinaemic, normogonadotrophic amenorrhoea. the control group consisted of 17 normal menstruating women between day 3 and 6 of the menstrual cycle. the mean age of the amenorrhoeic patients was 24.0 years (range 19 to 34) and the mean duration of amenorrhoea was 31 months (range 12 ...	1984	6428118
the hypothalamic-pituitary-thyroid axis in type 1 diabetes: influence of diabetic metabolic control.	the influence of diabetic metabolic control on indices of thyroid function was studied in 9 euthyroid, insulin-dependent (type 1) diabetics. during chronic poor metabolic control (mean fasting blood glucose 13 mmol/l and hba1 concentrations 14.7%) serum t3 concentrations were low (p less than 0.01) while serum t4 and basal tsh concentrations were normal. after 6-8 weeks of improved metabolic control, mean hba1 concentrations had fallen to 10.7% (p less than 0.01) and serum t3 concentrations had ...	1984	6428119
sex related goitrogenic effect of lithium carbonate in healthy young subjects.	eight healthy males and 8 healthy females without a family history of thyroid disease or detectable thyroid autoantibodies were given 300 mg of lithium carbonate twice a day for 28 days. using a precise and accurate ultrasonic technique we demonstrated an increase in median thyroid volume from 18 ml on day 0 to 34 ml on day 28 (p less than 0.005) in the female group, whereas the median thyroid volume was unchanged in the male group. a significant increase in basal serum tsh level and serum tsh l ...	1984	6428121
changes in circulating thyroid hormones during short-term hepatic enzyme induction with carbamazepine.	the effect of short-term hepatic enzyme induction with carbamazepine (cbz) on circulating thyroid hormone concentrations was studied in 10 healthy male subjects. cbz 400 mg per day was given for 21 days in 6 subjects and for 14 days in a further 4. in the former group the effect of therapy on the pituitary/thyroid axis was also assessed by measuring thyroid stimulating hormone (tsh) response to thyrotrophin-releasing hormone. cbz therapy resulted in induction of hepatic monooxygenase activity, e ...	1984	6428915
hypothalamo-adenohypophyseal-thyroid interrelationships in the chick embryo. v. the effects of thyroidectomy on t4 levels in blood plasma.		1984	6428972
thyroid status and nephron loss--a study in patients with chronic renal failure, end stage renal disease and/or on hemodialysis.	thyroid function was measured in 30 healthy subjects and 84 patients with various degrees of nephron loss (grf: 70 +/- 15 m/min, 30 +/- 16 ml/min, 10 +/- 7 ml/min and 2.1 +/- 1.3 ml/min). a low t3 and t4 syndrome is evident when grf is reduced to 30 +/- 16 ml/min while a blunted tsh response is detected earlier in the course of nephron loss. t3 response to trh was normal and ft4 was not affected by renal dysfunction. the data indicate that in nephron loss hypothalamic-pituitary abnormalities occ ...	1984	6429059
radioimmunoassays specific for the tertiary and primary structures of thyroxine-binding globulin (tbg): measurement of denatured tbg in serum.	antisera prepared by immunization of rabbits with human t4-binding globulin (tbg) contained two populations of antibodies: one directed against determinants of the native molecule, and the other directed against antigenic sites present only in denatured tbg. these two populations of antibodies were present in all nine antisera prepared in this or other laboratories that were tested. exploiting this property of anti-tbg sera and using radioiodinated denatured tbg as a tracer, a ria was developed ...	1984	6429183
hyperthyroidism due to inappropriate tsh secretion with associated hyperprolactinaemia--a case report and review of the literature.	a patient with inappropriate thyrotrophin (tsh) secretion is described. she initially presented with classical hyperthyroidism during pregnancy, responded to propylthiouracil and, subsequently, had a normal delivery. hyperthyroidism persisted and 7.5 months later a subtotal thyroidectomy was performed. after a further 16 months, mild symptoms of hyperthyroidism recurred. she again responded to propylthiouracil, but developed galactorrhoea. at that stage, it was noted that she had persistently el ...	1984	6429655
influence of pimozide on hypothalamo-pituitary function in children with behavioral disorders.	hypothalamo-pituitary functions were examined in thirteen children with behavioral disorders (six with hyperkinesia, four with autism, two with tic and one with schizophrenia) before and during treatment with pimozide, an antidopaminergic drug. the mean (+/- s.e.m.) basal serum prl level (24.5 +/- 4.2 ng/ml) during pimozide treatment was significantly higher than that (12.4 +/- 3.2 ng/ml) before treatment. hyperresponse of prl to tsh releasing hormone (trh) was observed in five (three with hyper ...	1984	6429690
responses of thoracic spinoreticular and spinothalamic cells to intracardiac bradykinin.	the response characteristics of spinoreticular and spinothalamic cells to intracardiac injection of bradykinin were determined in cats anesthetized with chloralose. extracellular potentials of individual spinoreticular and spinothalamic cells were recorded from the t2-t4 segments of the spinal cord. only those cells with cardiopulmonary sympathetic afferent input were tested for responses to intracardiac bradykinin (1-6 micrograms/kg). thirty-seven of the 64 cells studied responded to bradykinin ...	1984	6426311
effects of injections and/or chronic implants of melatonin and 5-methoxytryptamine on plasma thyroid hormones in male and female syrian hamsters.	plasma thyroid hormone concentrations in male and female syrian hamsters were evaluated in five experiments after two pineal indoles, melatonin (mel) and 5-methoxytryptamine (5-mt), were administered as chronic s.c. implants and/or daily afternoon injections. circulating concentrations of thyroxine (t4), ordinarily maintained by the long photoperiod (ld 14:10), were inhibited by daily afternoon injections of 25 micrograms mel but not 5-mt in male (experiment 1) and female (experiment 2) syrian h ...	1984	6593601
[wide excision in cancer of the tongue: the choice of approach, modalities of reconstruction].	surgical treatment of extensive carcinoma of the tongue requires reconsideration at the present time: firstly, because the efficacy of other therapeutic measures is rapidly suppressed by the size of the tumors, and there is not theoretical reason for excluding extensive surgery, and secondly, because esthetic and functional sequelae of these bloody treatments have been totally modified by the introduction of new reconstructive techniques. based on a series of 16 cases treated during a one-year p ...	1984	6593793
does glutathione regulate thyroxine deiodinase activity in cells?	since iodothyronine monodeiodinase require reduced sulfhydryl groups to be active, and since hepatic nonprotein sulfhydryls are low in states associated with decreased formation of t3 from t4, a regulatory role for glutathione (gsh) has been postulated in thyroid hormone deiodination. studies with cultured rat hepatocytes showed that a 90% decrease of total gsh, achieved by culturing cells in cys, met-deficient medium, did not alter 5'- or 5-deiodinase activity. the glutathione disulfide (gssg) ...	1984	6595189
biochemical characteristics of iodothyronine monodeiodination by rat liver microsomes: the interaction between iodothyronine substrate analogs and the ligand binding site of the iodothyronine deiodinase resembles that of the tbpa-iodothyronine ligand binding.	t4-analogs modified at the aliphatic side chain r are substrates for rat liver microsomal iodothyronine-5'(3')-deiodinase (ith-d). the variation of the substrate constitution allows a mapping of the ith-d substrate ligand-binding site. highest affinity for the ith-5'(3')-d was presented by tetrac among a homologous series of carboxylic acid side chain analogs. a net negative charge of the side chain and/or the blockage of the amino function increase the affinity of the enzyme-ligand interaction. ...	1984	6595191
peripheral autoregulation of thyroxine to triiodothyronine conversion in man.	this study describes the existence of a peripheral tissue mechanism for maintaining serum t3 concentrations in states of t4 deficiency or excess in man. serum t3 and t4 levels were determined in 242 primary hypothyroid subjects who had been maintained on varying doses of oral t4 therapy. subjects treated with sub-maintenance t4 doses were supplemented by additional oral t3 therapy to maintain eumetabolic status as assessed by suppression of serum tsh values. this t3 supplementation was withdrawn ...	1984	6595193
conversion of thyroxine to triiodothyronine in the anterior pituitary gland and the influence of this process on thyroid status.	there are two iodothyronine 5'-deiodinases in the rat anterior pituitary gland. the type ii 5'-deiodinase is responsible for the observable t4 to t3 conversion in pituitary tissue, both in vivo and in vitro. this type ii enzyme has kinetic characteristics which distinguish it from type i activity, and it is also insensitive to inhibition by propylthiouracil. iopanoic acid is both a substrate for, and an inhibitor of, the pituitary type ii 5'-deiodinase. studies in the rat and in man using iopano ...	1984	6595194
t3-hyperthyroidism caused by enhanced and shifted t4-conversion.	radioactivities of endogenously labelled thyroid hormones following in vivo application of 131 i and extraction from serial blood samples, show that t4 secretion is enhanced in t3-hyperthyroidism as it is in t4-t3-hyperthyroidism. in an extreme case of t3-hyperthyroidism with serum concentrations (sc) of t3 nearly equal to t4 (1000 ng/dl and 1800 ng/dl, respectively) tracer studies revealed a very short half life of t4 when compared to t3 (21.8 and 20.2 hrs., respectively). in 110 cases with bot ...	1984	6595195
malignancies in the aids patient: natural history, treatment strategies, and preliminary results.	almost 40% of the 3000 us patients with acquired immunodeficiency syndrome (aids) have a malignancy at the time of reporting, and the incidence of aids patients who develop a malignancy during the course of the disease may be 65-70%. 2 types of malignancies have been noted with increased frequency in aids victims: kaposi's sarcoma and malignant lymphomas. about 35% of all aids patients and 50% of homosexual or bisexual victims have developed kaposi's sarcoma, and those with coincident opportu ...	1984	6598311
internal quality control of hormone determinations by ria. detection of clinically significant analytical errors of serum thyroxine determination.	an internal quality control system by which clinically significant analytical errors can be detected with high probability was developed for serum thyroxine (t4) determination by ria. the mean concentration of the quality control samples (c1, c2, and c3) were 40.0, 101.8, and 156.7 nmol/l, and their total standard deviations (st) 2.5, 4.1, and 7.1 nmol/l, respectively, in stable analytical performance of the t4 assay. from the clinical point of view it was accepted that the results of c1 varied ...	1984	6599535
circadian and circannual rhythms of hormonal variables in elderly men and women.	a group of fourteen men (73 +/- 5 yr of age), and eighteen women (77 +/- 7 yr of age) institutionalized at the berceni clinical hospital, bucharest, romania, were studied over a 24-hr span once during each season (winter, spring, summer and fall). all subjects followed a diurnal activity pattern with rest at night and ate three meals per day with breakfast at about 0830, lunch at about 1300 and dinner at about 1830. the meals were similar, although not identical for all subjects during all seaso ...	1984	6600031
immunospecific binding of trna precursors containing n6-(delta 2-isopentenyl) adenosine.	antibodies specific for n6-(delta 2-isopentenyl) adenosine (i6a) were immobilized on sepharose and this adsorbent (sepharose-anti-i6a) was used to selectively isolate bacteriophage t4 trna precursors containing i6a/ms2i6a from an unfractionated population of 32p-labeled t4 rnas. the results showed that antibodies to i6a selectively bound only those trna precursors containing i6a/ms2i6a. binding of trna precursors by antibody and specificity of the binding was assessed by membrane binding using 3 ...	1984	6566571
thymus function in patients with resectable lung carcinoma.	a series of 43 patients (median age 57 years, range 39-73) underwent biopsy of the thymic area during resection for primary lung cancer (16 with negative nodes and 27 with positive nodes). in 22 cases in which study of lymphocyte subpopulations was possible t6 precursor thymocytes were found (mean 57%). t4 and t8 cells were present in proportions of 53% and 47%, respectively, equally distributed between t6 and t3 cells. a well-defined lobular structure was found in 11 cases and active thymic tis ...	1984	6567476
goitre and thyroid dysfunction during chronic amiodarone treatment.	we measured thyroid function in a cross-sectional survey of 37 unselected patients receiving chronic amiodarone treatment. palpable goitre was presented in 17 patients and was a new finding in ten. despite frequent elevations of serum free t4 (67%) or free t4 index (43%), all 37 patients were clinically euthyroid with a normal or decreased serum free t3 or free t3 index. mean urine iodide/creatinine excretion was increased 13-fold. three patterns of thyroid function were seen; in 21 patients wit ...	1984	6585712
t-lymphocyte t4 molecule behaves as the receptor for human retrovirus lav.	many viruses, including retroviruses, are characterized by their specific cell tropism. lymphadenopathy-associated virus (lav) is a human lymphotropic retrovirus isolated from patients with acquired immune deficiency syndrome (aids) or related syndromes, that displays selective tropism for a subset of t lymphocytes defined by the expression of a surface glycoprotein of relative molecular mass 62,000 (62k) termed t4 (refs 6-8). this glycoprotein delineates a subset of t lymphocytes with mainly he ...	1984	6083454
prophylaxis and treatment of endemic goitre in western sudan with intramuscular iodized oil.	a single injection of iodized oil was administered intramuscularly in a dose of 0.2-2 ml (475 mg iodine per ml) to 383 children from the villages of kas and dibbis of the darfur region in western sudan, where goitre is prevalent. after one year the prevalence of goitre had declined from 76% to 49.6%. of the goitrous children, 81.1% showed either complete resolution of goitre or definite regression of its size. none of the nongoitrous children developed goitre and none of the existing goitres bec ...	1984	6084463
studies on the regulation of rna labeling and on the biochemical constituents of normal and abnormal human thyroid.	the present studies were performed in order to analyze the incorporation of 3h-uridine into rna and its hormonal regulation as well as the content of total iodine, t3, t4, rna, dna and protein in normal and abnormal human thyroid obtained at surgery. the content of protein, rna and dna is not significantly changed in follicular adenoma and in adenomatous goiter, rna labeling from 3h-uridine showed similar values in slices from normal and abnormal glands. tsh and cyclic amp increased the labeling ...	1984	6085710
specific labeling of protein domains with antibody fragments.	monovalent antibody fab fragments, prepared from antisera raised against two different types of crystalline arrays made of either intact, or a proteolytic fragment of bacteriophage t4 major capsid protein, gp23, were employed to stoichiometrically label different gp23 protein domains on the outer surface of a tubular variant (i.e., "polyheads") of bacteriophage t4 capsids. computer filtrations of both negatively stained and freeze-dried/metal-shadowed specimens permitted approximate mapping of ...	1984	6085811
the effect of thyroid hormone on the high affinity ca2+-atpase in rat liver plasma membrane.	the effect of thyroid hormone on the high affinity ca2+-atpase activity in rat liver plasma membrane was studied. the high affinity ca2+-atpase activity in plasma membrane was activated by 10(-7)-10(-5) m of ca2+ and was inhibited by 70 microm trifluoperazine. thyroidectomy of rats was associated with an increase in the activity of high affinity ca2+-atpase. the increased enzyme activity was normalized by t4 administration to the animals. on the other hand, na+-k+-atpase activity in the membrane ...	1984	6086294
identification of the breakage-reunion subunit of t4 dna topoisomerase.	the antitumor drug 4'-(9-acridinylamino)methanesulfon-m-anisidide which stimulates the cleavable complex formation between mammalian dna topoisomerase ii and dna also stimulates the cleavable complex formation between bacteriophage t4-induced dna topoisomerase and dna. in the presence of 4'-(9-acridinylamino)methanesulfon-m-anisidide, t4 dna topoisomerase and dna form a "cleavable complex" which is characterized by its sensitivity to protein-denaturant treatment. upon protein-denaturant treatmen ...	1984	6086624
changed promoter specificity and antitermination properties displayed in vitro by bacteriophage t4-modified rna polymerase.	a 5.5 x 10(3) base-pair fragment of bacteriophage t4 dna carrying genes 1, 3, 57, ipi and a cluster of transfer rna genes was used as template for rna polymerase isolated from uninfected escherichia coli and from t4-infected bacteria. rna transcripts were fractionated by gel electrophoresis and mapped by using as transcription template the 5.5 x 10(3) base fragment cleaved with different restriction enzymes. the comparison of the transcripts synthesized by the two rna polymerases revealed a dram ...	1984	6086941
genetic and physiological studies of an escherichia coli locus that restricts polynucleotide kinase- and rna ligase-deficient mutants of bacteriophage t4.	the rna ligase and polynucleotide kinase of bacteriophage t4 are nonessential enzymes in most laboratory escherichia coli strains. however, t4 mutants which do not induce the enzymes are severely restricted in e. coli ctr5x, a strain derived from a clinical e. coli isolate. we have mapped the restricting locus in e. coli ctr5x and have transduced it into other e. coli strains. the restrictive locus seems to be a gene, or genes, unique to ctr5x or to be an altered form of a nonessential gene, sin ...	1984	6086961
amniotic fluid endocrine changes during maternal hyperalimentation.	ten women with low estriol excretion received hyperalimentation prior to induction of labor. six received an amino acid mixture (5% aminofusin) and 25% dextrose, two received the amino acid mixture, and two received 25% dextrose. amniotic fluid obtained before and after hyperalimentation was assayed for fetal surfactant production, thyroid, pituitary, and carbohydrate regulating hormones. in the combined amino acid/dextrose infusion group the amniotic fluid palmitic acid levels increased signifi ...	1984	6086968
the effect of the 3',5' thiophosphoryl linkage on the exonuclease activities of t4 polymerase and the klenow fragment.	the 3'----5' exonuclease activities of t4 dna polymerase and the klenow fragment of polymerase i towards the phosphoryl and thiophosphoryl 3',5' linkage were examined under comparable conditions of idling-turnover, duplex hydrolysis and turnover during polymerization. with the t4 enzyme there is a negligible effect of thiosubstitution on these activities; with the klenow fragment there is a greater than one hundred-fold reduction in rate with the thiolinkage for the exonuclease but not polymeriz ...	1984	6087297
effects of reproductive state on concentrations of thyroxine, 3,5,3'-triiodothyronine and cortisol in serum of dogs.	reproductive state of animals frequently is overlooked when examining endocrine functions of the thyroid gland and adrenal cortex. this experiment was done to determine effects of reproductive state on basal and stimulated concentrations of thyroxine (total t4), 3,5,3'-triiodothyronine (total t3) and cortisol in serum of adult beagle dogs. five male, 5 anestrous, 5 proestrous, 5 diestrous, 5 pregnant and 5 lactating dogs were fasted for 18 h before each dog received 5 iu of thyrotropic hormone ( ...	1984	6087944
pharmacokinetic and pharmacodynamic basis for the treatment of metastatic breast cancer with high-dose medroxyprogesterone acetate.	postmenopausal patients with metastatic breast cancer were treated with medroxyprogesterone acetate (mpa) (clinovir) in dosages between 500 and 1500 mg orally per day. the relation of mpa plasma concentrations and endocrine effects were studied in a longitudinal fashion. mpa exerted suppressive effects on the basal and gonadotropin-releasing hormone (gnrh) stimulated gonadotropin secretion, cortisol, dehydroepiandrosterone (dhea), and estradiol (e2) in a dose-dependent manner leading to a comple ...	1984	6088020
stimulation of rat renal na+, k+-atpase activity by thyroid hormones.	the effect of thyroid hormones (t4, t3 and reverse t3) on rat renal na+,k+-atpase activity was investigated by a cytochemical technique. t3 caused stimulation of na+,k+-atpase activity in the renal medulla but not in the renal cortex. there was a peak in enzyme activity after cultured renal segments had been exposed to t3 for 11 min and this time of maximal stimulation did not vary with the concentration of t3. a rectilinear response in na+,k+-atpase activity was observed over t3 concentration r ...	1984	6088111
excision repair of pyrimidine dimers from simian virus 40 minichromosomes in vitro.	the ability of dna repair enzymes to carry out excision repair of pyrimidine dimers in sv40 minichromosomes irradiated with 16 to 64 j/m2 of uv light was examined. half of the dimers were substrate for the dna glycosylase activity of phage t4 uv endonuclease immediately after irradiation, but this limit decreased to 27% after 2 h at 0 degrees c. moreover, the apyrimidinic (ap) endonuclease activity of the enzyme did not incise all of the ap sites created by glycosylase activity, although all ap ...	1984	6088490
interactions of the nuclear thyroid hormone receptor with core histones.	these studies concern the interactions of the rat liver thyroid hormone nuclear receptor with histones and factors influencing the receptor's assay and stability. heating certain crude receptor preparations at 50 degrees c produces a selective loss of triiodothyronine (t3) but not thyroxine (t4) binding activity, whereas, with more purified preparations, such heating decreases both t3 and t4 binding. the selective t3-binding loss in crude preparations was found to be due to the simultaneous dena ...	1984	6088522
the biochemical basis of 5-bromouracil-induced mutagenesis. heteroduplex base mispairs involving bromouracil in g x c----a x t and a x t----g x c mutational pathways.	we have investigated the mechanism of bromouracil-induced transition mutations in vitro using synthetic dna templates and purified t4 dna polymerase. evidence is presented for the occurrence of bromouracil-guanine base pairs in product dna in the g x c----a x t pathway where guanine is present in the dna template and bromouracil is present as the deoxynucleoside triphosphate substrate 5-bromodeoxyuridine triphosphate. this finding supports a widely known but as yet untested model proposed by fre ...	1984	6088545
site specific mutagenesis: insertion of single noncomplementary nucleotides at specified sites by error-directed dna polymerization.	we have utilized infidelity of dna synthesis as a basis for site-directed mutagenesis. both an endonuclease restriction fragment and a synthetic oligonucleotide were used as primers. dna polymerase from bacteriophage t4 was used to elongate primer termini to a position immediately adjacent to two different preselected positions on phix174 dna templates. then, the error-prone dna polymerase from avian myeloblastosis virus was used to insert single non-complementary nucleotides at the designated p ...	1984	6089123
demonstration of the production of frameshift and base-substitution mutations by quasipalindromic dna sequences.	the in vivo production of frameshift and base-substitution mutations predicted as a consequence of the metabolic processing of misaligned quasipalindromic dna sequences has been confirmed. spontaneous frameshift mutations of the t4 rii gene that had been genetically mapped to quasipalindromic dna sequences were sequenced. some of the mutant sequences are exactly those predicted by a mutational model based on misaligned quasipalindromes. furthermore, these sequences are distinct from those predic ...	1984	6089210
hormonal influence on endothelial cell angiotensin-converting enzyme activity.	the influence of various hormones on angiotensin-converting enzyme (ace) production and release by bovine endothelial cells in culture was studied. dexamethasone, thyroxine (t4), and triiodothyronine (t3) stimulated ace activity in cells and their culture supernatants without affecting cell number or protein content. the stimulating effects of dexamethasone and thyroid hormones were additive, suggesting that these hormones may have different sites of action. in addition, their stimulating effect ...	1984	6089573
monoclonal antibody analysis of mononuclear cells in myopathies. ii: phenotypes of autoinvasive cells in polymyositis and inclusion body myositis.	in 6 cases of polymyositis and 6 of inclusion body myositis, phenotypes of mononuclear cells focally surrounding and invading muscle fibers were analyzed. by localizing the t8, t4, and ia markers with direct immunofluorescence and acid phosphatase enzyme cytochemically in the same sections, five different phenotypes were simultaneously identified in a given section: t8+ and t4+ cells that were either activated (ia+) or not activated (ia-), and acid phosphatase--reactive and ia+ macrophages. this ...	1984	6089646
hormonal regulation of prolactin receptors in male rat target tissues: the effect of hypothyroidism and adrenalectomy.	the effects of hypothyroidism and adrenalectomy (adx) on prl receptors as well as the subcellular distribution of prl-binding sites in adult male rat target tissues were investigated. in ventral prostate, kidney, and adrenal, prl-binding activity was enriched in the 15,000 x g pellet (mitochondrial/golgi fraction). the zona fasiculata/reticularis plus medulla of the adrenal had higher prl-binding activity than the zona glomerulosa (plus capsule). propylthiouracil-induced hypothyroidism significa ...	1984	6090097
photoaffinity labeling of thyroid hormone nuclear receptors. influence of n-butyrate and analysis of the half-lives of the 57,000 and 47,000 molecular weight receptor forms.	the thyroid hormone receptor is a nuclear-associated protein which appears to mediate the actions of 3,5,3'-triiodo-l-thyronine (l-t3) and 3,5,3',5'-tetraiodo-l-thyronine (l-t4) in mammalian cells. in a previous study we reported that n-2-diazo-3,3,3-trifluoropropionyl-3,5,3'-triiodo-l-thyronine (l-t3-pal) serves as an effective photoaffinity label probe of the receptor in gh1 cells, a growth hormone producting rat pituitary cell line. irradiation of cells at 254 nm covalently cross-links l-[125 ...	1984	6090453
phenotype and function of engrafted maternal t cells in patients with severe combined immunodeficiency.	engrafted maternal t cells from two patients with severe combined immunodeficiency (scid) and graft-vs-host disease (gvhd) were characterized for surface phenotype, function, and ecto-5'-nucleotidase (ecto-5'-nt) activity. the majority of engrafted t cells from both patients were t6-, t3+, and ia+; the ratio of t4+:t8+ cells varied from 0.89 to 3.1 for patient 1 and was 0.17 for patient 2. the sum of t4+ + t8+ cells was greater than the number of t3+ cells, and approximately one-third of the pat ...	1984	6090535