Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| hypothyroidism of hypothalamic origin in pyridoxine-deficient rats. | pyridoxine-deficient young rats (3 weeks old) had significantly reduced levels of pituitary tsh, serum thyroxine (t4) and tri-iodothyronine (t3) compared with pyridoxine-supplemented rats. the status of the pituitary-thyroid axis of normal, pyridoxine-supplemented and pyridoxine-deficient rats was evaluated by studying the binding parameters of [3h](3-methyl-histidine2)trh in the pituitary of these rats. the effects of trh and t4 injections on pituitary tsh and serum tsh, t4 and t3 of these two ... | 1986 | 3090182 |
| in vitro restoration by interleukin-2 (il-2) of the impaired natural killer cell activities, il-2 receptor expression, and t cell proliferation in hemophilia. | five of 22 hemophiliacs who were seropositive for human t cell leukemia virus iii (htlv iii) and manifested severe impairment of immune parameters (both in vivo and in vitro) similar to those observed in patients with clinical symptoms of acquired immune deficiency syndrome (aids) were chosen for this study. profound lymphopenia was observed in four of five patients with decreased and qualitatively impaired helper/inducer (t4) cells and increased t suppressor/cytotoxic (t8) cells. observed in al ... | 1986 | 3090209 |
| thyroid hormones and experimental cancer cachexia. | the relationship between circulating thyroid hormones and nutritional status was studied in sarcoma-bearing inbred c57bl/6j mice and control mice. supplementation with exogenous thyroxine (t4) was also evaluated. tumor-bearing animals had depressed levels of circulating thyroid hormones. this was also found in food-restricted (pair-fed and pair-weighed) controls. plasma levels of thyroid hormones decreased with increased tumor burden. thyrotropin-releasing hormone caused an increased response of ... | 1986 | 3090341 |
| [lactotropic and thyrotropic functions of the hypophysis in polycystic ovary syndrome]. | an increase in the prolactin basal level was detected in 8 out of 50 patients with ovarian polycystosis. a test with trh and a simultaneous determination of the levels of prolactin, tth, t3, t4 were used in 25 patients, of them a hyperergic reaction of prolactin to trh was detected in 5. a correlation analysis of function of the thyroid was performed. positive correlation between the levels of prolactin and tth in patients with hyperprolactinemia and between a maximum increment in percentage to ... | 1986 | 3090541 |
| long-term effects of trh administration on food intake and body weight in the rat. | the effect of long-term trh administration through drinking water (0.2 mg/ml) on food and water intake and body weight has been studied in three groups of female rats: (1) thyroidectomized, (2) thyroidectomized receiving daily 250 micrograms/kg of l-t4, (3) sham-operated. treatment with oral trh for 30 days decreased body weight and increased food intake in sham-operated rats. no trh effects on body weight or food consumption were observed in either of the other groups of thyroidectomized rats. ... | 1986 | 3090567 |
| effect of short-term lithium carbonate administration in hyperthyroidism, with or without associated ophthalmopathy. | the study was carried out on 21 female hyperthyroid patients aged 22-53 years, with or without associated ophthalmopathy, admitted to the endocrinology ward of the 1st medical clinic of the craiova county hospital, romania, between 1983 and 1985. administration of lithium carbonate was proceeded and followed by radioimmunoassay (ria) determinations of t3 and t4 and measurement of achilles' reflex (ar). two regimens were employed. the first comprised ten patients receiving doses of 500 mg/day lit ... | 1986 | 3090679 |
| serum concentrations of 3,5,3',5'-tetraiodothyroacetate (t4a) in subjects with hypo-, hyper- and euthyroidism. | some factors influencing serum 3,5,3',5'-tetradiodothyroacetate (t4a) concentrations were examined in hypothyroid, euthyroid and hyperthyroid human subjects. serum t4a concentration was shown to be correlated with parameters such as serum, total and free thyroxine (t4) concentrations and thyroxine: thyroxine binding globulin ratio, which are indicative of the availability of t4 for peripheral metabolism. the relationship between t4a and these parameters was not a simple linear one; serum levels ... | 1986 | 3090812 |
| different effects of il-2 addition or antibody crosslinking on t-cell subset stimulation by cd3 antibodies. | the effect of exogenous recombinant interleukin-2 (il-2) or of antibody crosslinking on the activation of human t-cell subsets by igg2a (okt3/bma030), igg1 (leu4 and ucht1), or igg2b (bma031) anti-t3 antibodies (cd3) was investigated. in so-called nonresponder cultures as well as in monocyte-depleted cell cultures addition of il-2 increased the cd3-induced activation and proliferation of t4 and t8 cell subsets. relatively more t8 than t4 cells were stimulated by antibody binding and il-2. crossl ... | 1986 | 3091264 |
| an improved approach to thyroid function testing in patients with non-thyroidal illness. | we have compared the results of serum thyrotrophin (tsh) measurements using a sensitive immunoradiometric assay (irma) with those of conventional thyroid function tests in 299 hospital inpatients with a range of non-thyroidal illnesses. levels of total thyroxine (t4), free t4, total tri-iodothyronine (t3) and free t3 in the hypothyroid range were recorded in 8%, 15%, 19% and 49% of patients, respectively, whereas tsh (irma) was abnormally low in 1%. furthermore, basal tsh (irma) accurately predi ... | 1986 | 3091294 |
| cultured human thymus epithelial monolayer cells induce cd4 expression on mononuclear cells of aids patients in vitro. | we investigated the in vitro effect of cultured human thymic epithelial monolayer cells on mononuclear cells (mnc) from patients with acquired immune deficiency syndrome (aids) and aids-related complex (arc). patients having undergone bone-marrow transplantation (bmt), who showed a similar deficiency of t-lymphocytes expressing cd4 (leu-3, t4), and healthy blood donors served as controls. most epithelial monolayer cells were of thymic medulla origin, as documented using a panel of monoclonal ant ... | 1986 | 3091303 |
| effects of in vivo bolus versus continuous trh administration on tsh secretion, biosynthesis, and glycosylation in normal and hypothyroid rats. | the effects of in vivo trh administered either as bolus or continuous doses on tsh secretion, synthesis, and glycosylation were studied in normal and hypothyroid rats. nine-week-old normal or 3-week postthyroidectomy rats were administered bolus doses of saline or trh (0.5 mg/kg) twice daily or continuous saline or trh (1 mg/kg/day) via an osmotic pump. after 5 days, pituitaries were removed and incubated with [35s]methionine (met) and [3h]glucosamine (glcn), with or without 10(-8) m trh, for 6 ... | 1986 | 3091425 |
| time-dependent effects of oral thyrotropin-releasing hormone in hyperthyroid female rats. | we have studied the thyrotropin (tsh) and thyroid hormone (t3) response to acute thyrotropin-releasing hormone (trh) administration in rats previously rendered hyperthyroid by daily ip injection of 25 micrograms l-t4/100 g bw for 30 days. animals were thereafter put on distilled water or trh (2 mg/10 ml) as drinking solution for another 18 days, continuing t4 administration. on days 3 and 10 on oral trh, the serum tsh and t3 response to trh was studied by administering 2 micrograms of the tripep ... | 1986 | 3091472 |
| sustained rises in serum thyrotropin, thyroxine, and triiodothyronine during long term, continuous thyrotropin-releasing hormone treatment in patients with amyotrophic lateral sclerosis. | in a pilot therapeutic trial, four patients with amyotrophic lateral sclerosis (als) were treated with long term, continuous infusions of trh, three intrathecally and one epidurally. they had prompt increases in serum tsh and thyroid hormone concentrations, averaging 120% for tsh, 49% for serum t4, 68% for the serum free t4 index, 49% for serum t3, and 67% for the serum free t3 index. these elevations were statistically significant for all but serum t3 and persisted for the duration of treatment ... | 1986 | 3091628 |
| leu-3/t4 expression on epidermal langerhans cells in normal and diseased skin. | recent evidence exists that the expression of the leu-3/t4 antigen is not restricted to thymus-derived lymphocytes but can also be detected on mononuclear phagocytes and epidermal langerhans cells (lc). when searching for the presence of leu-3/t4 antigen-bearing cells in tissue sections of a variety of inflammatory and neoplastic skin disorders, we observed quantitative and qualitative differences in the intensity of anti-leu-3a labeling of epidermal dendritic cells. reasoning that leu-3/t4 expr ... | 1986 | 3091703 |
| a case of total thyroxine-binding globulin deficiency with graves' disease: fluctuations of plasma triiodothyronine/thyroxine ratio. | a 37-year-old male with total thyroxine-binding globulin (tbg) deficiency associated with graves' disease is described. both tbg immunoreactivity and tbg capacity were not detectable in his serum. serum concentrations of thyroxine-binding prealbumin and albumin were normal. he was initially hyperthyroid. during methimazole-treatment he was maintained in an euthyroid state except for two short hypothyroid periods. his plasma triiodothyronine/thyroxine (t3/t4) ratios during both the untreated hype ... | 1986 | 3091890 |
| sigma factors from e. coli, b. subtilis, phage sp01, and phage t4 are homologous proteins. | we show, using dot matrix comparisons and statistical analysis of sequence alignments, that seven sequenced sigma factors, e. coli sigma-70 and sigma-32, b. subtilis sigma-43 and sigma-29, phage sp01 gene products 28 and 34, and phage t4 gene product 55, comprise a homologous family of proteins. sigma-70, sigma-32, and sigma-43 each have two copies of a sequence similar to the helix-turn-helix dna binding motif seen in crp, and lambda repressor and cro proteins. b. subtilis sigma-29, sp01 gp28, ... | 1986 | 3092189 |
| a role for t3+4-6-8- transitional thymocytes in the differentiation of mature and functional t cells from human prothymocytes. | in vivo, immunocompetent t lymphocytes are only detected late in ontogeny, among mature thymocytes expressing either t4 (l3t4 in mouse) or t8 (lyt-2) surface glycoproteins. we have previously shown, however, that there are functional precursors among t3+4-6-8- human thymocytes in vivo. here we report on the in vitro differentiation of prothymocytes into t3+4-6-8- and mature t cells. t11+3-4-6-8- prothymocytes (0.5% of total thymocytes, greater than 98% pure) were obtained after treatment of thym ... | 1986 | 3092222 |
| distinction of two cd3-monoclonal antibodies in respect to the effectivity to modulate the induction of igm- and gamma-interferon synthesis. | monoclonal antibodies which specifically bind to epitopes within the t3-antigen complex (cd3 mab) are excellent tools to analyse mechanisms of t-cell activation and of the interactions between t-cells, b-cells, accessory cells and intermediating immunefactors. in the present study we could show, that the cd-3 mabs bma 030 and bma 031 are highly effective in modulating immunereactions, similar to antibodies directed to the t4-antigen (cd4-mab) or the t8-antigen (cd8-mabs) respectively. the data s ... | 1986 | 3092794 |
| immunoblastic lymphadenopathy, angioimmunoblastic lymphadenopathy, and ibl-like t-cell lymphoma. a spectrum of t-cell neoplasia. | thirty cases of immunologically determined and histologically diagnosed immunoblastic lymphadenopathy (ibl), angioimmunoblastic lymphadenopathy (aild), and ibl-like t-cell lymphoma were clinicopathologically reviewed. clinical manifestations and laboratory findings did not reveal significant differences in these three groups. ibl, aild, and ibl-like t-cell lymphoma showed a spectrum of histologic changes, in which proliferation of pale cells was a critical diagnostic point for the histologic mal ... | 1986 | 3093047 |
| the role of il-2 and t4+ cells in the generation of human influenza virus-specific ctl activity. | stimulation of human peripheral blood lymphocytes (pbl) with influenza a virus leads to the generation of virus-specific cytotoxic t lymphocyte (ctl) activity as well as natural killer (nk)-like activity. in this study, we show that exogenous il-2 augments the in vitro generation of virus-specific ctl activity, only when added some days after the initiation of the culture. apparently, the endogenously produced il-2 can be a limiting factor in the in vitro generation of ctl activity. the increase ... | 1986 | 3093087 |
| human thymocyte maturation in vitro: a flow cytometric analysis. | using an in vitro culture system, light scatter analyses, and two-color flow cytometry, we provide evidence that the interleukin-2 (il-2) and transferrin receptors can be induced within 48 hr on nonproliferating immature thymocytes. the thymocytes (greater than 35%) that expressed the transferrin and il-2 receptors demonstrated nuclear activation as measured by log 90 degrees light scatter analysis. increases in antigen-receptor-associated t3-antigen expression followed transferrin and il-2-rece ... | 1986 | 3093098 |
| effect of glibenclamide on thyroid hormone metabolism in rats. | this study was undertaken to elucidate the effect of glibenclamide, one of sulfonylurea drugs, on thyroid hormone metabolism in vivo and on the conversion of thyroxine (t4) to 3,5,3'-triiodothyronine (t3) in the isolated perfused rat liver and kidney. glibenclamide (0.2 mg/kg body weight) was intraperitoneally administered to normal and streptozotocin-induced (50 mg/kg) diabetic rats for 14 days. the liver and kidney of normal rats were perfused for 30 minutes with a synthetic medium containing ... | 1986 | 3093349 |
| the influence of hemoglobin level on the regression and long term local control of transitional cell carcinoma of the bladder following photon irradiation. | patients with transitional cell carcinoma of the bladder treated by radical megavoltage x ray therapy are analyzed. there was a significant correlation between tumor t stage classification and the hemoglobin level at the start of radical radiotherapy. a hemoglobin level of 12g/dl or more was associated with a significant improvement in complete local tumor regression at 6 months and durable local tumor control for patients with t3 cancer or a cancer of grade 3 histology. hemoglobin level did not ... | 1986 | 3093414 |
| inherited heat-stable variant thyroxine-binding globulin (tbg-chicago). | the five known types of inherited variant t4-binding globulin (tbg) have in common increased sensitivity to heat denaturation compared to the common type tbg (tbg-c). in the course of studies to screen for and characterize variant tbgs in population groups, we detected a unique tbg with marked resistance to heat denaturation. the propositus was a 22-year-old black man without personal or family history of thyroid disease. his native tbg (ntbg) in serum had a t1/2 of denaturation at 56 c of 90 mi ... | 1986 | 3093522 |
| phenotypic identification of mononuclear cells in oral premalignant lesions and cancer by monoclonal antibodies. | to explore the nature and importance of mononuclear cells of different phenotypes in oral premalignant lesions and oral cancer, we studied biopsy specimens from 21 oral red and/or white lesions (6 hyperkeratosis, 3 mild dysplasia, 4 severe dysplasia and 8 squamous cell carcinoma), using monoclonal antibodies and avidin-biotin-peroxidase complex staining. peripheral blood samples (pb) from 4 normal subjects and 5 reactive lymph nodes (ln) were used as controls for the technique. t11-positive cell ... | 1986 | 3093654 |
| immune responses during human schistosomiasis mansoni. xiii. immunological status of spleen cells from hospital patients with hepatosplenic disease. | splenocytes from 25 patients with severe hepatosplenic schistosomiasis mansoni were obtained after therapeutic splenectomy. spleen cells were phenotyped and analysed for responsiveness to mitogens or heterogeneous schistosome-derived antigenic preparations (eggs, sea; adult worms, swap; cercariae, cerc) in blastogenesis assays and lymphokine production systems, and were compared with peripheral blood mononuclear cells (pbmn). splenic lymphocytes were 55% t lymphocytes (sheep erythrocyte rosette- ... | 1986 | 3094136 |
| [prealbumin-associated hyperthyroxinemia]. | prealbumin associated hyperthyroxinaemia represents a rare but important protein binding anomaly, causing difficulties in differential diagnostics. this anomaly is based on a variant prealbumin showing an increased affinity to t4 and rt3 and in some cases also when using t4 analogue tracers. the condition of the patient is clinically euthyroid which is confirmed by a normal btsh level, a normal response to trh, and peripheral parameters (bmr, asr, sti, shbg) in the euthyroid range. this paper de ... | 1986 | 3094315 |
| pituitary-thyroid function and thyrotropin, prolactin and growth hormone responses to trh in patients with chronic alcoholism. | basal plasma concentrations of thyroxine (t4), 3,3',5-triiodothyronine (t3), free t4 index (tf4i), free t3 index (ft3i) reverse t3, 3,3',5-triiodothyronine (rt3), resin t3 uptake (tr3u), thyroxine-binding globulin (tbg), thyrotropin (tsh), prolactin (prl) and growth hormone (gh) as well as thyrotropin releasing hormone (trh) stimulated tsh, prl and gh were investigated in 31 consecutive male patients (mean age 41 years) with chronic alcoholism. according to the histology of their liver biopsies ... | 1986 | 3094323 |
| [value of the triiodothyronine suppression test with serum t4 and thyroid 131i uptake as indexes in judging the prognosis of graves' disease]. | 1986 | 3094869 | |
| the t4 gene encodes the aids virus receptor and is expressed in the immune system and the brain. | the isolation of clones encoding the human surface protein t4, and the expression of the t4 gene in new cellular environments, have enabled us to examine the role of this protein in the pathogenesis of aids. our studies support a mechanism of aids virus infection that initially involves the specific interaction of the aids virus with t4 molecules on the cell surface. this association can be demonstrated on t4+ transformed t and b lymphocytes as well as epithelial cells. furthermore, the presence ... | 1986 | 3094962 |
| inhibition of follicle-stimulating hormone induction of aromatase activity in porcine granulosa cells by thyroxine and triiodothyronine. | we examined the effects of t3 and t4 on basal and fsh-induced aromatase activity in granulosa cells isolated from medium-sized follicles (4-6 mm) of prepubertal pigs. treatment of cells with t3 or t4 alone during an initial 48-h induction period did not result in any significant change in aromatase activity, as measured by 17 beta-estradiol accumulation during the subsequent 6-h test period, when testosterone (0.5 microm) was added as substrate. however, when cells were cultured with fsh and t3 ... | 1986 | 3095107 |
| accessory cell-t cell interactions involved in anti-cd3-induced t4 and t8 cell proliferation: analysis with monoclonal antibodies. | the effect of monoclonal antibodies (mab) directed at t cell and accessory cell (ac) surface molecules on okt3-induced t4 and t8 cell proliferation was examined. mab directed at nonpolymorphic class i (w6/32, mb40.5) and class ii (l243) major histocompatibility complex (mhc)-encoded gene products, an epitope common to lfa-1, cr3, and the p150, 95 molecule (60.3), and a heterodimer present on monocytes (m phi) and activated t cells (4f2) inhibited m phi-supported okt3-induced proliferation of bot ... | 1986 | 3095421 |
| the immunoregulatory nature of iron. ii. lymphocyte surface marker expression. | previously, we presented preliminary evidence that supported our hypothesis for the immunoregulatory nature of iron [7]. the objective of the present work was to test that hypothesis in greater detail. our approach was to examine the effect that iron had on the expression of the surface markers on lymphocytes that had been activated by pokeweed mitogen (pwm). the two categories of lymphoid surface molecules were enumerated on those cells; first were those that identify t lymphocytes and second, ... | 1986 | 3095475 |
| diagnostic value of free triiodothyronine in serum. | serum free t3 concentration has been assessed in various thyroid conditions by a t3 analog method and the results compared with those obtained by equilibrium dialysis in the same individuals. the methodology is easy to perform and reproducible. ft3 determination appears to be especially valuable in detecting borderline thyrotoxicosis as in cases previously cured from thyrotoxicosis but suspected of relapse, or in nontoxic goitrous patients overtreated with t4. | 1986 | 3095510 |
| the effect of double filtration plasmapheresis on thyroid hormone economy and thyroid function. | five cases of rheumatoid arthritis (ra), two cases of systemic lupus erythematosus (sle), a case of mixed connective tissue disease (mctd), and a case of cold urticaria were treated with double filtration plasmapheresis (dfp). each aliquot of plasma was obtained at three different points of the dep circuit during the treatment and concentrations of thyroid hormones as well as thyroxine binding globulin (tbg) were measured. despite the removal of considerable amounts of triiodothyronine (t3), thy ... | 1986 | 3095574 |
| alterations in t4 (cd4) protein and mrna synthesis in cells infected with hiv. | cells infected with the human immunodeficiency virus (hiv) show decreased expression of the 58-kilodalton t4 (cd4) antigen on their surface. in this study, the effect of hiv infection on the synthesis of t4 messenger rna (mrna) and protein products was evaluated in t-cell lines. metabolically labeled lysates from the t4+ cell line sup-t1 were immunoprecipitated with monoclonal antibodies to t4. compared with uninfected cells, hiv-infected sup-t1 cells showed decreased amounts of t4 that coprecip ... | 1986 | 3095925 |
| thyroid function in prematures with respiratory distress syndrome (rds). | reports on thyroid function in newborns with respiratory distress syndrome (rds) are controversial, the significance of obtained results is not clear. therefore we conducted a longitudinal study of thyroid function in 35 infants with rds (gestational age 24-36 weeks, birth weights 650-2770 g). 43 well prematures, matched for gestational age, served as controls. no significant differences were observed in cord blood tsh, t3, t4, tbg values and t4:tbg ratios between infants with and without rds. p ... | 1986 | 3096040 |
| juvenile thyrotoxicosis. | the incidence of juvenile thyrotoxicosis has not exceeded one case per 100,000 population per year in hungary during the past decades. from more than 200 simultaneous determinations of serum thyroid hormones (t3, t4, ft4) it was concluded that increased ft4 concentrations are the most frequent findings in thyrotoxicosis, even when both or one of the other thyroid hormone concentrations (t3, t4) are normal. tsh and prolactin responses to trh were studied in 18 patients; tsh remained always undete ... | 1986 | 3096041 |
| single-strand binding proteins from phage t4 and e. coli form higher order structures with poly(dt). | complexes of poly(dt) with gene 32 protein from phage t4 or e. coli single-strand binding protein were digested by nuclease p1 from penicillum citrinum. protected fragments were analyzed by gel electrophoresis. in both cases, a series of bands was obtained corresponding to multiples of a repeat unit whose size was about 80 nucleotides. such protected fragments could not be detected under the same experimental conditions when poly(da) was used instead of poly(dt). the formation of nucleosome-like ... | 1986 | 3096391 |
| human gamma interferon enhances release from phytohemagglutinin-stimulated t4+ lymphocytes of activities that stimulate colony formation by granulocyte-macrophage, erythroid, and multipotential progenitor cells. | human gamma interferon (huifn gamma) was evaluated for its effects on the release from human peripheral blood t lymphocytes (greater than 98% pure) stimulated by phytohemagglutinin (pha) of activities that can stimulate granulocyte-macrophage (cfu-gm) colonies and clusters, erythroid (bfu-e) bursts, and mixed (cfu-gemm) colonies. t lymphocytes did not release these activities in the absence of pha with or without huifn gamma. in the presence of pha, pure natural huifn gamma at concentrations of ... | 1986 | 3096401 |
| discrepancy between uptake of serum triiodothyronine as measured by albumin and charcoal methods in nonthyroidal illnesses. | triiodothyronine (t3) uptake as determined with albumin (at3u) and with charcoal (ct3u) as binders were compared for patients with low-t3 syndrome. both t3u values were identical in normal subjects and in patients with thyroidal disorders, pregnancy, low thyroxin-binding globulin (tbg), or acute hepatitis with high concentration of tbg. in the low-t3 syndrome, at3u was significantly higher than ct3u (38.5 +/- 7.2% vs 29.4 +/- 4.9%, mean +/- sd, n = 37, p less than 0.001), and this difference inc ... | 1986 | 3096613 |
| phenotypic characterization of individual interferon-gamma-producing cells after okt3 antibody activation. | this is the first study using a direct approach to determine the phenotype of interferon gamma (ifn-gamma)-producing cells after polyclonal t cell activation. blood mononuclear cells (mnc) were cultured and stimulated by okt3 antibody to produce ifn-gamma. they were then stained with a panel of monoclonal antibodies with specificity for cell surface antigens, fixed and permeabilized in suspension and stained for cytoplasmic ifn-gamma using monoclonal antibodies and indirect immunofluorescence te ... | 1986 | 3096747 |
| genetic control of the production of anti-thyroid hormone antibodies in mice immunized with human thyroglobulin. | various strains of mice with different h-2 and igh-i allotypes were immunized with human thyroglobulin (htg) and genetic control of the production of anti-thyroid hormone antibodies was examined. anti-htg antisera obtained from different strains of mice were examined for the presence of anti-thyroid hormone antibodies. at least one ir-gene which controls the production of anti-t4 antibodies by immunization with htg was identified in the i-a subregion. the presence of ir-genes outside the h-2 was ... | 1986 | 3096882 |
| reduced affinity for thyroxine in two of three structural thyroxine-binding prealbumin variants associated with familial amyloidotic polyneuropathy. | t4-binding prealbumin (tbpa), a protein synthesized by the liver, circulates as a tetramer and transports 15-20% of t4. we studied 3 variants of the tbpa monomer recently identified in serum and amyloid fibrils of patients affected by familial amyloidotic polyneuropathy (fap). they represent single amino acid substitutions at positions 30 (type i), 60 (appalachian), and 84 (type ii). tests of thyroid function and the apparent association constant (ka) of t4 binding to tbpa were measured in whole ... | 1986 | 3097057 |
| effects of intravenous trh on growth hormone and cortisol serum levels in children and adolescents with insulin dependent diabetes mellitus. | the effects of an iv thyrotropin releasing hormone (trh) bolus on serum growth hormone (gh) and cortisol levels were evaluated in 59 children and adolescents with insulin dependent diabetes mellitus (iddm) and in 24 healthy, age-matched control subjects. in the iddm group gh baseline levels sharply rose within 30 min after trh and successively normalized. on the contrary, trh injection failed to affect gh serum concentrations in the control group. the gh increase after trh in iddm patients was p ... | 1986 | 3097114 |
| human interferon alpha and gamma production by lymphocytes during the generation of influenza virus-specific cytotoxic t lymphocytes. | we analysed the production of interferons (ifn)-alpha and -gamma during the generation of human influenza-virus specific cytotoxic t lymphocyte (ctl) responses using monoclonal antibodies in a specific radioimmunoassay. the results showed that the peripheral blood mononuclear cells (pbm) of all donors tested produced ifn-gamma and had influenza a virus-specific ctl activity after stimulation. the amount of ifn-gamma produced and the level of ctl activity were significantly correlated. the pbm of ... | 1986 | 3097247 |
| octapeptides deduced from the neuropeptide receptor-like pattern of antigen t4 in brain potently inhibit human immunodeficiency virus receptor binding and t-cell infectivity. | the differentiation antigen t4, present on the helper/inducer subset of t lymphocytes, is thought to serve as the receptor for the human immunodeficiency virus (hiv). we find that a 60-kda protein, immunoprecipitable by monoclonal antibody (mab) okt4, is present on membranes from human brain as well as human t cells. furthermore, the radioiodinated hiv envelope glycoprotein [125i-labeled gp120 (125i-gp120)] can be specifically covalently affixed to a molecule present on rat, monkey, and human br ... | 1986 | 3097649 |
| [biochemical, hematologic, immunologic and cytogenetic study of papillary dermatitis in fattened pigs]. | examinations were performed of 31 fattened pigs suffering from papular dermatitis (pd) and 33 fattened pigs without dermal changes (c) coming from the d. large pig-fattening farm and the production breeding herd of pigs. the weight of the pigs was from 95 to 105 kg. both farms are sited in a mining area (intensive lignite extraction). the pd pigs, compared with the controls (c), had less total protein, cholesterol and calcium in their blood serum and increased concentrations of thyroxine (t4), n ... | 1986 | 3097911 |
| decreased serum thyroglobulin levels in the late stage of pregnancy. | serum thyroglobulin levels were serially measured in 25 normal pregnant women to evaluate thyroidal activity during normal pregnancy. measurements included serum t3, t4, free t4, tbg, and tsh. tg and ft4 levels were found to be decreased in the third trimester when compared with those of the first trimester and with those of normal non-pregnant individuals (p less than 0.01). tsh levels were higher than normal in pregnant women at all stages of pregnancy, with a significant rise at the third tri ... | 1986 | 3098016 |
| changes in blood glucose, liver glycogen, ketone bodies and plasma insulin in suckling rats treated with a single high cortisol dose one day after birth. | rats treated with cortisol, 0.5 mg one day after birth, show a decrease in body and organ weight and a decrease in pituitary tsh and gh when compared to controls together with a lack of response of tsh to trh. these alterations have been previously found in animals treated with large doses of thyroxine, neo-t4 syndrome, in both populations a brain underdevelopment has been observed. plasma insulin in cortisol-treated animals was higher (p less than 0.01) than in the controls at 8 and 12 days of ... | 1986 | 3098019 |
| serum thyroid hormones and thyroid hormone binding proteins in patients with completed stroke. | thirty euthyroid patients hospitalized for completed stroke had serum thyroxine, triiodothyronine, thyroxine-binding globulin and thyroxine-binding prealbumin levels significantly decreased (p less than 0.01 to p less than 0.001) compared with 70 age- and sex-matched healthy controls. admission serum thyroxine levels gave the best correlation with mortality (t4 less than 65 nmol/l = 100% mortality; 65-77 nmol/l = 38%; 78-90 nmol/l = 9%; greater than 90 nmol/l = 0%). in general, during hospitaliz ... | 1986 | 3098157 |
| 3,5-diiodotyrosine and thyronine in the urine of patients with chronic renal disease. | urinary 3,5-diiodotyrosine (dit) and thyronine (t0) excretion was investigated in 18 patients with chronic renal disease. in accord with previous findings serum t4 and thyroid hormone binding proteins measured in 17 patients were in the low or normal range. urinary albumin excretion was elevated in all 18 and t4 binding prealbumin (tbpa) in 15 of the 18. urinary t0 excretion measured in 12 patients was also significantly lower than normal (mean +/- sd 4.4 +/- 2.6 vs 15.8 +/- 5.8 nmol/24 h renal ... | 1986 | 3098453 |
| hypothalamic hypopituitarism following cranial irradiation for nasopharyngeal carcinoma. | eight patients, one male and seven females, with no pre-existing hypothalamic-pituitary disease, who developed symptoms of hypopituitarism following cranial irradiation for nasopharyngeal carcinoma were studied 5 years or more after radiotherapy. all were gh deficient. four of the patients with no gh response during insulin tolerance tests (itt) showed increased gh in response to synthetic human growth hormone releasing factor (grf-44). four patients had impaired cortisol responses to itt, and g ... | 1986 | 3098456 |
| growth hormone responses to grf 1-29 in patients with primary hypothyroidism before and during replacement therapy with thyroxine. | it is well known that hypothyroidism is frequently associated with impaired gh responses to different stimuli. in the present study we have evaluated gh responses to gh-releasing factor (grf) in patients with primary hypothyroidism before and during t4 replacement therapy. fourteen patients (age range 26-60 years) underwent two grf tests (1 microgram/kg) before and during replacement therapy (150 micrograms/d). administration of t4 increased peak gh responses to grf in 9 patients and in the grou ... | 1986 | 3098458 |
| alterations in the hypothalamic-pituitary-thyroid axis after prolonged residence in antarctica. | the human population which lives and works in polar environments has been increasing steadily over the last 15 years. very little is known about how these residents adjust to their environment. cold adaptation in man is a poorly understood phenomenon. euthermic mammals maintain body temperature during cold exposure via non-shivering thermogenesis, a process which is hormonally mediated. we studied prospectively the response of the hypothalamic-pituitary-thyroid axis in 17 euthyroid men before, d ... | 1986 | 3098460 |
| effects of six weeks' neuroleptic treatment on the pituitary-thyroid axis in schizophrenic patients. | in order to investigate the effects of 6 weeks' neuroleptic treatment on the pituitary-thyroid axis in 25 male schizophrenic patients, and the diurnal variation in the thyrotropin (tsh) and prolactin (prl) responses to thyrotropin-releasing hormone (trh) in these patients, the trh stimulation test was performed in each of them at 14.00 and 24.00 h of the same day, both before and after 6 weeks' treatment with neuroleptics (chlorpromazine or fluspirilene). also, serum thyroxine (t4), in vitro rad ... | 1986 | 3108695 |
| coronal sections of larynges from radiation-therapy failures: a clinical-pathologic study. | serial coronal sections of 89 wide-field laryngectomies were examined. twenty were specimens obtained from laryngectomies to treat patients for whom primary radiation therapy failed to cure early laryngeal cancer. these specimens were compared to 69 specimens from laryngectomies for t3 and t4 laryngeal cancers. the irradiation-failure group showed a significantly greater invasion of cartilage and extension into subglottic areas. the extension of tumors along blood vessels and mucous glands appea ... | 1986 | 3108762 |
| postmortem endocrine levels in the vitreous humor. | the vitreous humor and sera from 51 autopsy cases were assayed for progesterone, estradiol, thyroxine, triiodothyronine, tsh, fsh, lh and prolactin. the results indicate that the blood retinal barrier has unique properties of its own. all hormones were measurable in the sera. progesterone, estradiol, t3 and t4 were not detected despite their lipid solubility and their small molecular size while the larger glycoprotein hormones were easily measureable. | 1986 | 3105417 |
| grades 1 and 2 hypothyroidism in a state mental hospital: risk factors and clinical findings. | thirty-six chronically psychotic patients (nine men and 27 women, mean age 56.7 +/- s.d. 13.4 years) were found to have elevated thyroid stimulating hormone (tsh) levels during review of thyroid function screening tests of 1150 patients over a 15 month period in a 700 bed state mental hospital. this study population of 36 patients was more likely to be female and older than the general hospital population. the spectrum and frequency of psychiatric diagnoses included dementia (3); schizoaffective ... | 1986 | 3104944 |
| binding stoichiometry of the gene 32 protein of phage t4 in the complex with single stranded dna deduced from boundary sedimentation. | short 145 base dna fragments in complex with the helix destabilizing protein of bacteriophage t4, gp32, have been studied with boundary sedimentation. the sedimentation coefficient was determined as a function of concentration, protein-nucleic acid ratio, temperature and salt concentration. it can be concluded that the measured values reflect the properties of the saturated dna-gp32 complex. a combination of the earlier obtained translational diffusion coefficient of the complex with the sedimen ... | 1986 | 3271416 |
| extrathyroidal conversion of thyroxine to 3,5,3'-triiodothyronine in cold-acclimated thyroxine-maintained thyroidectomized rats. | chronic exposure of rats to cold (4 degrees c) leads to thyroid gland hyperactivity as a compensatory mechanism for activating body heat production. there is increased extrathyroidal production of t3 from t4 in parallel to thyroid hormone hypersecretion. since the 5'-deiodination (5'-d) of t4 can be modulated by thyroid hormones, it has been suggested that the increased thyroid hormone secretion may activate the 5'-d enzymatic pathway leading to increased extrathyroidal t3 production. in an atte ... | 1986 | 3828577 |
| toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin in cold-adapted rats. | the toxicity of 60 micrograms/kg 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) given ip in corn oil/5% acetone was examined in male sprague-dawley rats adapted to 25 degrees c or 4 degrees c ambient temperature. cold exposure significantly reduced mean time to death and tended to increase mortality. body weight at the time of death was reduced at both ambient temperatures to about the same extent. thus, the rate of body weight loss was about twice as fast in non-survivors at 4 degrees c than at 25 ... | 1986 | 3827590 |
| regulation of ca2+-dependent protein turnover in skeletal muscle by thyroxine. | dantrolene, an agent that inhibits ca2+ mobilization, improved protein balance in skeletal muscle, as thyroid status was increased, by altering rates of protein synthesis and degradation. thyroxine (t4) caused increases in protein degradation that were blocked by leupeptin, a proteinase inhibitor previously shown to inhibit ca2+-dependent non-lysosomal proteolysis in these muscles. in addition, t4 abolished sensitivity to the lysosomotropic agent methylamine and the autophagy inhibitor 3-methyla ... | 1986 | 3827846 |
| [carcinomas of the posterior pharyngeal wall. experience of the institut curie. analysis of the results of radiotherapy]. | between 1960 to 1982, 98 patients with squamous cell carcinomas of the posterior pharyngeal wall were treated at the curie institute by radiotherapy alone. the absolute survival is 30% and 18% at three and five years. the major cause of death is loco-regional failure. 95% of the local failures arrived within the 18 first months. the 2 years control rate ranged from 80% for t1, 64% for t2, to 36% for t3 and 22% for t4. the local control is so depending on the radiation induced regression of the p ... | 1986 | 3827078 |
| combined phenytoin and salicylate effects on thyroid function tests. | to evaluate the effects of salicylates (asa) on thyroid function tests (tft's) in patients already taking phenytoin (dph), 6 adults received a daily dose of dph to achieve a steady state serum dph. asa was then added stepwise (325, 650, 975 mg q 4hr) at 2-day intervals. tfts, total serum and free salivary dph were measured. therapeutic steady state phenytoin levels caused a significant decrease in serum free thyroxine (ft4), total t4, total t3 (tt3) and a significant but slight increase in t3ru. ... | 1986 | 3827407 |
| influence of a single neonatal melatonin treatment on the basal and thyrotropin or melatonin modified blood thyroxine level of adult rats. | neonatal melatonin treatment caused a significant increase in thyroxine (t4) level at one month of age. preexposure to melatonin in neonatal age and reexposure after one month accounted for a still greater increase in t4 production. rats neonatally exposed to melatonin did not respond to tsh at one month of age. | 1986 | 3825556 |
| the helmstein bladder distension treatment for tumours and severe bleeding. | the helmstein bladder distension treatment was applied to 26 patients, who received a total of 70 treatments (52 for bladder tumour and 18 for severe bleeding; in 7 cases for both tumour and bleeding). in the t2 tumours total necrosis occurred in 27% of the cases, partial necrosis in 50% and no necrosis in 23%. of the patients with total necrosis 64% had recurrences within 12 months. in the t3-t4 tumours only 33% had a temporary slight reduction of the tumour mass. in patients with severe bleedi ... | 1986 | 3818218 |
| plasma free and total iodothyronine levels in the newborn lamb. physiological considerations. | neonatal changes in plasma total and free iodothyronine levels were monitored in 18 limousin x romanov suckling lambs (experiment 1) and 24 limousin x romanov animals bottle-fed in standardized conditions (experiment 2). in the two experiments, plasma free t3 levels were closely related to total t3 levels, whereas some differences could be observed between total and free plasma t4 levels. the neonatal rise in free t4 in particular was higher than that observed for total t4 levels. consequently, ... | 1986 | 3823603 |
| [relationship of the value of excess body weight to the blood concentration of cholesterol and triglycerides and the level of the basic hormones regulating lipid metabolism in healthy men 30 to 50 years old]. | the basal levels of cholesterol (cs), triglycerides (tg), sugar, immunoreactive insulin (iri), cortisol, testosterone, triiodothyronine (t3) and thyroxine (t4) were compared in 116 healthy males with risk factors of developing coronary heart disease (chd), aged 45, engaged in administrative activities associated with stress and hypokinesia, with relation to normo-, hyperlipoproteinemia (nlp and hlp) and percentage of excessive body mass (ibm). in nlp as well as in hlp with an increase in ibm a t ... | 1986 | 3824209 |
| effects of intraamniotically administered thyroxin on acceleration of fetal pulmonary maturity in preeclamptic toxemia. | in 18 cases of serious and fairly serious toxemic pregnancy, the authors gave 500 micrograms l-thyroxin "henning" intraamniotically after the laboratory evaluation demonstrated negative pulmonary maturity from an amniotic fluid sample. maternal and fetal complications due to the drug could not be observed. despite a 39% premature incidence, irds and hyaline membrane disease were not observed. the administered t4 caused a positive change in the direction of the l/s ratio and the clements test. th ... | 1986 | 3820031 |
| adaptation of male and female rats to iodine deficiency. | the response of the hypothalamic pituitary axis to chronic iodine deficiency was compared in male and female sprague-dawley rats. the animals were kept on a low iodine diet for 12 weeks. blood samples as well as thyroid and pituitary weights were obtained every two weeks. baseline values of thyroid weight and serum thyroxine (t4) were similar in both sexes. however, females had lower serum tsh and higher serum triiodothyronine (t3), pituitary weight and pituitary tsh content. after initiation of ... | 1986 | 3817747 |
| congenital goiter sustaining normal level of serum triiodothyronine. | we attempted to elucidate the deficient site of thyroid hormone biosynthesis in the thyroid gland and the mechanism of sustaining normal t3 level in sera of a patient with congenital goiter. ty, a 8-yr-old boy, first noted the onset of a diffuse goiter at the age of 2. there was no clinical evidence of hypothyroidism except for the slight impairment of intellectual development and the awkward physical activity. bmr, t3-rsu and t4 showed low values (-13%, 20.8% and 2.2 micrograms/dl), but serum t ... | 1986 | 3817751 |
| non-steroid hormones and tissue polypeptide antigen (tpa) in emetic and non-emetic pregnancy. | in order to investigate further the endocrine and metabolic features of the common condition emesis gravidarum, serum concentrations of some non-steroid hormones and tissue polypeptide antigen (tpa) were determined in 102 healthy pregnant women. 62 complained of nausea and vomiting in early pregnancy. significantly higher and lower levels of human chorionic gonadotropin were noted in early and late pregnancy, respectively, in women with emesis gravidarum. a significant rise in serum prolactin an ... | 1986 | 3811848 |
| thyroid hormone receptors in human breast cancer: effect of thyroxine administration. | the present study was designed to establish the presence and pharmacological properties of the nuclear triiodothyronine receptors (t3r) (binding capacity, affinity and number of sites) in human breast cancer samples as well as the effects on them of thyroid hormone administration (t4). a computerized scatchard plot analysis was designed to resolve the exponential curves in order to estimate the real number of t3r. t3r was present in 83% of the patients studied. in the thyroxine treated group, th ... | 1986 | 3813491 |
| [treatment of neonatal hypothyroidism. comparative effects of levothyroxine and thyroid extracts]. | the authors compare the effects of treatment with thyroid extracts and levothyroxine on t3, t4 and tsh levels in 2 groups of 8 hypothyroid infants. serum tsh levels were lower in infants receiving thyroid extracts on and after the 15th day. the interest of a treatment associating t4 and t3 and of adapted doses of lt4 is discussed. | 1986 | 3813782 |
| autologous rosette forming cells in subjects at risk for the acquired immunodeficiency syndrome. | we investigated the distribution of autologous rosette forming cells (arfc) in the peripheral blood from subjects at risk for the acquired immunodeficiency syndrome (aids). the mean percentage of arfc with autologous plasma from 35 male homosexual individuals was significantly lower than that of 31 normal controls. the mean percentage of arfc of sara had a direct linear correlation with the percentage of t4+ cells (p less than 0.01). within the sara group, those with antibodies against htlv-iii/ ... | 1986 | 3814262 |
| plasma concentrations of corticosterone and thyroid hormones in laying fowls from different housing systems. | plasma concentrations of corticosterone, thyroxine (t4) and triiodothyronine (t3) were measured in representative mature female domestic fowls from three housing systems: cages, covered strawyard and range. there were no between-system differences for t4 but t3 was lower in birds from range than in those from cages. corticosterone concentrations were lower in birds from strawyards than in those from range and cages. in the strawyards there were no differences in corticosterone concentrations bet ... | 1986 | 3815130 |
| hepatic damage in the rat following administration of thyroxine or triiodothyronine, assessed by measurement of plasma glutathione s-transferase yaya concentrations. | a specific radioimmunoassay (ria) has been developed that has sufficient sensitivity to allow measurement of the changes in plasma and tissue glutathione s-transferase (gst) yaya concentrations which occur following thyroid hormone administration in the rat. using the ria it was demonstrated that the only tissues that had significant amounts of gst yaya were liver, small gut and kidney. administration of triiodothyronine (t3) or thyroxine (t4) resulted in increases in plasma gst yaya concentrati ... | 1986 | 3815855 |
| serum t3 and reverse t3 concentrations: indices of metabolic control in diabetes mellitus. | several previous studies have reported that serum t3 and reverse t3 (rt3) concentrations are altered in uncontrolled diabetes mellitus and their normalization occurs when euglycemia is achieved. therefore, this study was undertaken to examine the usefulness of serum t3 and rt3 levels as indices of metabolic control in diabetes mellitus. serum t3, rt3, t4, free t4, tsh and t3 resin uptake were determined in 18 normal subjects and 35 patients with newly discovered diabetes mellitus before initiati ... | 1986 | 3816044 |
| abnormal in vitro immunoglobulin synthesis of lymphocytes and increased circulating immune complex in patients with ankylosing spondylitis. | to explore the immunopathogenetic mechanisms of hypergammaglobulinemia in ankylosing spondylitis (as), the functions of b cell, helper t (okt4) cell and suppressor t (okt8) cell were examined, by using the co-cultivation technique for in vitro immunoglobulin biosynthesis, in 7 patients with active as, 7 with inactive as and 14 normal controls. circulating immune complexes (cic) in those subjects were also measured by a solid phase c1q binding assay. after b, okt4 and okt8 cells were separated fr ... | 1986 | 3816353 |
| [value of free, microvascular anastomosis of a small intestine transplant in reconstructive surgery following resection of t4 tumors of the upper aerodigestive tract]. | the jejunal graft with microsurgical vascular anastomoses is now recognised as one of the most reliable methods for reconstructing large and various defects of the upper aero-digestive tract. after resection of t4-tumours and reconstruction of phonation, deglutition and aesthetics 50% of our patients could be resocialized within the first postoperative month. | 1986 | 3807600 |
| [study of atp-independent stages of reaction catalyzed by phage t4 rna-ligase]. | the isotope exchange between [5'-32p]pap and a(5')ppap catalyzed by enzyme was shown not to take place in the absence of the acceptor; i. e. the necessity of the acceptor presence during the second step of the process was demonstrated. the isotope exchange reaction between [5'32p]pap and (pa)5p was studied. it was demonstrated that acceptor (pa)4, slightly whereas the acceptor (pu)4 completely inhibits the isotope reaction. the isotope reaction exchange between [5'-32p]pap and (pu)4pap does not ... | 1986 | 3807908 |
| primary hypothyroidism with paradoxically elevated plasma thyroxine due to thyroxine-binding antibody. | investigation of an elderly patient with clinical hypothyroidism revealed a low serum triiodothyronine (t3) but markedly raised levels of total thyroxine (tt4) and free thyroxine (ft4) when measured by radio-immunoassay (ria). primary hypothyroidism was suggested by an elevated basal thyroid stimulating hormone (tsh) and confirmed by a low ft4 when measured by a commercial microencapsulated antibody method. the paradoxically elevated levels of ft4 and tt4 were due to the presence of an antibody ... | 1986 | 3809074 |
| dna sequence of the tail fiber genes 37, encoding the receptor recognizing part of the fiber, of bacteriophages t2 and k3. | the dna sequences of genes 37 of bacteriophages t2 and k3 are presented and compared with that of phage t4. the corresponding proteins constitute, as dimers, the part of the long tail fibers that recognizes the bacterial receptor. the co2h termini of the polypeptides are located at the free ends of the fibers. morphologically, the three phages are essentially identical, but they use different receptors. the genes from phages t4, t2 and k3 encode proteins consisting of 1026, 1341 and 1243 amino a ... | 1986 | 3806672 |
| spectrum of spontaneous frameshift mutations. sequences of bacteriophage t4 rii gene frameshifts. | the dna sequences of 185 independent spontaneous frameshift mutations in the riib gene of bacteriophage t4 are described. approximately half of the frameshifts, including those at hot spot sites, are fully consistent with classical proposals that frameshift mutations are produced by a mechanism involving the misaligned pairing of repeated dna sequences. however, the remaining frameshifts are inconsistent with this model. correlations between the positions of two base-pair frameshifts and the bas ... | 1986 | 3806675 |
| management of the undescended thyroid. | ectopic thyroid can occur throughout the neck but is found most commonly at the tongue base. management includes tc99m radionuclide scanning to confirm the presence and location of the thyroid tissue. dysfunction should be assessed by t4, tsh, and thyroid antibody testing. hormonal therapy is the primary treatment but surgery may be necessary in cases of airway obstruction. six children with undescended thyroid are presented and a management protocol based on the presence or lack of symptomatolo ... | 1986 | 3806772 |
| thyroid hormone metabolism by glial cells in primary culture. | the metabolism of thyroxine (t4) and triiodothyronine (t3) in cultured glial cells was studied in situ. cultures were prepared from fetal rat brain and grown for the last 4 days in a chemically defined medium (cdm). they contained astrocytes and oligodendrocytes as shown by the enzyme markers, glutamine synthetase and 2',3'-cyclic nucleotide phosphohydrolase. these cells contained high affinity (22-33 pm), limited capacity (120-230 fmol/mg dna) nuclear receptors for t3. cells incubated in situ w ... | 1986 | 3803706 |
| exploratory behavior, learning ability, and thyroid hormonal responses to stress in female rats rehabilitating from postnatal hypothyroidism. | newborn female long-evans rats were divided into groups of normal, hypothyroid [0.1% propylthiouracil (ptu) a reversible antithyroid goitrogen in the litter's drinking water], and hypothyroid rehabilitated (ptu water from birth to day 25, normal water thereafter). the rats were tested for several adaptive behavioral tasks between 40 and 90 days of age. at day 50, serum concentration of tsh and thyroid hormones revealed no detectable amounts of t4 and a 10-fold increase in tsh in the hypothyroid ... | 1986 | 3803723 |
| the region of phage t4 genes 34, 33 and 59: primary structures and organization on the genome. | the product of gene 33 is essential for the regulation of late transcription and gene product 59 is required in recombination, dna repair and replication. the exact functions of both proteins are not known. restriction fragments spanning the genomic area of genes 33 and 59 have been cloned into phage m13 and a 4.9 kb nucleotide sequence has been determined. translation of the dna sequence predicted that gp33 contains 112 amino acids with a mol.wt. of 12.816 kd while gp59 is composed of 217 amino ... | 1986 | 3797242 |
| triiodothyronine (t3) nuclear binding sites in human placenta and decidua. | we studied the binding of triiodothyronine (t3) to human placenta and decidua. although the placenta effectively blocks transfer of thyroxine (t4) and t3 it may itself be a thyroid hormone-dependent tissue and may specifically bind t3. a single class of high-affinity binding sites was found (ka 3.8 +/- 0.31 s.e.m., and 3.3 +/- 10(9) m-1) for both placenta and decidua. limited capacity was also observed, viz., 0.117 +/- 0.01 and 0.102 +/- 0.018 fmoles/microgram deoxyribonucleic acid (dna), respec ... | 1986 | 3797387 |
| 3,5,3'-triiodothyronine (t3) and 3,3',5'-triiodothyronine (rt3) synthesis in rats hosting the r3230ac mammary tumour. | generation of t3 and rt3 from t4 was studied in r3230ac mammary tumours grown in fischer 344 rats as well as in the liver and kidney of these tumour-bearing hosts. the primary objective of this study was to determine if reversion of t3 to rt3 synthesis occurs in this experimental tumour model and in organs remote from the tumour site. tumours, hepatic and renal homogenates were analyzed 14-16 days following tumour implantation for 5- and 5'-iodothyronine deiodinase activity using thyroxine as su ... | 1986 | 3797959 |
| ligation of single-stranded oligodeoxyribonucleotides by t4 rna ligase. | despite its unique ability to ligate single-stranded dna molecules, t4 rna ligase has so far seen little use in molecular biology due to long reaction times, modest yields, and apparent inability to promote ligation of long oligodeoxyribonucleotides. we describe here a set of reaction conditions which dramatically shorten the reaction time and give reproducible 40 to 60% ligation of dna fragments of up to 40 bases in length. these improvements open promising new fields of application to t4 rna l ... | 1986 | 3799962 |
| effect of fasting on thyroxine, 3,5,3'-triiodothyronine, and cortisol concentrations in serum of dogs. | the present study was designed to compare basal and stimulated concentrations of 3,5,3'-triiodothyronine (t3), thyroxine (t4), and cortisol in serum of dogs fasted 12 or 18 hours (to represent overnight fasting) or 24 or 36 hours (to represent prolonged inappetence) with those of dogs that were not fasted. twenty-five adult beagle bitches were allotted to 5 experimental fasting groups (0, 12, 18, 24, and 36 hours). blood samples for hormonal analyses were obtained 4, 3, 2, and 1 hour before food ... | 1986 | 3800108 |
| histopathological characteristics predictive for growth of squamous cell carcinomas of the head and neck heterotransplanted to nude mice. | human tumours heterotransplanted to nude mice vary both with respect to take rate and the rate of tumour line establishment, according to their histological type and between primary and recurrent tumours. the acceptance rate of squamous cell carcinomas of the head and neck is also thought to vary, according to their degree of differentiation and their site of origin. this provided the basis of the present analysis of different tumour characteristics predicting growth of squamous cell carcinomas ... | 1986 | 3800324 |
| a time-course study of hypothyroidism-induced hypotension: its relation to hypothermia. | dietary administration of propylthiouracil (0.2%) to male sprague-dawley rats for a period of 8 weeks induced hypothyroidism and hypotension in these animals. resting heart rate, body weight, colonic temperature and serum thyroxine (t4) were significantly lower in the treated hypothyroid rats than in the controls. time-course study indicates that the maximal depression of these parameters occurred about the eighth week of ptu treatment; except t4 level which declined abruptly about the second we ... | 1986 | 3800508 |
| induction chemotherapy with cis-platinum and 5-fluorouracil for squamous cell carcinoma of the head and neck. | one hundred and eight patients with squamous cell carcinoma of the upper aerodigestive tract (uadt) (t3, t4, no-n3; 17% stage ii, 54% stage iii, 27% stage iv) were given three courses of chemotherapy before any local treatment. the regimen consisted of cis-platinum 100 mg m-2 on day 1 and 5-fluorouracil 1000 mg m-2 on days 2-6; drugs were administered by continuous infusion. the toxicity of this protocol was acceptable, as 82% of the patients were able to receive the initially scheduled drug dos ... | 1986 | 3801272 |
| cell-mediated immunity in brazilian hemophilia patients. | the purpose of this study was to correlate the immunological features of healthy hemophiliac a patients treated with commercial cryoprecipitates with those who received factor viii concentrates associated or not with cryoprecipitates. the absolute number of total lymphocytes, t3, t4 and b lymphocytes did not differ for either group of patients or the controls. the number of t8 lymphocytes was higher in the group treated only with cryoprecipitates than for the controls. the t4/t8 lymphocyte ratio ... | 1986 | 3801725 |
| a rat model of the 131i-induced changes in thyroid function. | thyrotoxic patients treated with iodine-131 (131i) often present with low thyroxine (t4), normal triiodothyronine (t3) and raised thyrotropin (tsh) concentrations in serum. we have developed a rat model of this low t4, raised tsh state. rats were injected with 50, 150 or 450 mu ci 131i. a dose of 50 mu ci 131i caused no significant effect on thyroid function, as assessed by serum parameters whereas both 150 mu ci and 450 mu ci 131i caused a significant fall in serum t4 concentration accompanied ... | 1986 | 3794182 |
| thyroid hormone metabolism in thyroid disease as reflected by the ratio of serum triiodothyronine to thyroxine. | in 77 euthyroid subjects the mean ratio between serum triiodothyronine (s-t3) in nmol/l x 10(3) and serum thyroxine (s-t4) in nmol/l was 22.4 with a standard deviation of 4.3. the distribution of the ratio was close to gaussian. it was not changed in subjects under estrogen effect. the ratio was increased in hypothyroid subjects suggesting a mechanism preserving s-t3 better than s-t4 in thyroid failure. this mechanism seemed independent of tsh as the increasing s-t3/s-t4 ratio in primary hypothy ... | 1986 | 3794185 |
| the electroencephalographic pattern during electroconvulsive therapy. iv. spectral energy distributions with methohexital, innovar and ketamine anesthesias. | during phase iii of nondominant unilateral ect seizures, total energy, total peak energy, total delta energy, and 1/2-power duration of total delta energy are all less (shorter) in the unstimulated, dominant hemisphere than they are in the stimulated hemisphere. interhemispheric differences in total energy are greatest in the lateral frontotemporal cortex, where they average 40 percent with both methohexital and ketamine anesthesias in f8 greater than f7 and t4 greater than t3. this circumstance ... | 1986 | 3791648 |