Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
molecular phylogenetic diversity of bacillus community and its temporal-spatial distribution during the swine manure of composting.in order to obtain the diversity and temporal-spatial distribution of bacillus community during the swine manure composting, we utilized traditional culture methods and the modern molecular biology techniques of polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and -denaturing gradient gel electrophoresis (pcr-dgge). bacillus species were firstly isolated from the composting. based on temperature changes, the temporal-spatial characteristics of total culturable bacill ...201121701982
composition and in-ávitro antibacterial activity of essential oils from four satureja species growing in iran.the composition and in-ávitro antibacterial activity of essential oils from four satureja species (s. bachtiarica, s. khuzistanica, s. mutica and s. rechingeri) growing in iran were determined. according to the results of gc-fid and gc-ms analysis, all oil samples were principally composed of phenolic constituents (carvacrol and/or thymol) with the percentages ranging from 41.2% (s. bachtiarica) to 77.7% (s. rechingeri). determining antibacterial activities by the disc diffusion method against t ...201121827283
role of the five rna helicases in the adaptive response of bacillus cereus atcc 14579 cells to temperature, ph, and oxidative stresses.in this study, growth rates and lag times of the five rna helicase-deleted mutants of bacillus cereus atcc 14579 were compared to those of the wild-type strain under thermal, oxidative, and ph stresses. deletion of cshd and cshe had no impact under any of the tested conditions. deletion of csha, cshb, and cshc abolished growth at 12┬░c, confirming previous results. in addition, we found that each rna helicase had a role in a specific temperature range: deletion of csha reduced growth at all the ...201121705526
controlled delivery of gentamicin using poly(3-hydroxybutyrate) microspheres.poly(3-hydroxybutyrate), p(3hb), produced from bacillus cereus spv using a simple glucose feeding strategy was used to fabricate p(3hb) microspheres using a solid-in-oil-water (s/o/w) technique. for this study, several parameters such as polymer concentration, surfactant and stirring rates were varied in order to determine their effect on microsphere characteristics. the average size of the microspheres was in the range of 2 ++m to 1.54 ++m with specific surface areas varying between 9.60 m(2)/g ...201121845079
compact dry(r) x-bc for the enumeration of bacillus cereus in food samples.we evaluated the effectiveness of using compact dry(r) x-bc (cd-xbc), a ready-to-use and self-diffusing dry medium sheet culture system based on a novel detection principle, for the detection and enumeration of bacillus cereus. all 13 b. cereus strains, which were studied for the inclusivity study, grew as blue/green colonies on the cd-xbc. when 3 yeast strains and 103 bacterial strains other than b. cereus were tested for the exclusivity study, 5 strains formed white colonies, and 4 strains for ...201121719993
chemical constituents and antibacterial activity of melastoma malabathricum l.the aqueous methanolic extracts of melastoma malabathricum l. exhibited antibacterial activity when assayed against seven microorganisms by the agar diffusion method. solvent fractionation afforded active chloroform and ethyl acetate fractions from the leaves and the flowers, respectively. a phytochemical study resulted in the identification of ursolic acid (1), 2+¦-hydroxyursolic acid (2), asiatic acid (3), +¦-sitosterol 3-o-+¦-d-glucopyranoside (4) and the glycolipid glycerol 1,2-dilinolenyl-3 ...201121834640
investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.).the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ...201121822040
Biochemical and Mutational Studies of the Bacillus cereus CECT 5050T Formamidase Support the Existence of a C-E-E-K Tetrad in Several Members of the Nitrilase Superfamily.Formamidases (EC 3.5.1.49) are poorly characterized proteins. In spite of this scarce knowledge, ammonia has been described as playing a central role in the pathogenesis of human pathogens such as Helicobacter pylori, for which formamidase has been shown to participate in the nitrogen metabolic pathway. Sequence analysis has revealed that at least two different groups of formamidases are classified as EC 3.5.1.49: on the one hand, the derivatives of the FmdA-AmdA superfamily, which are the best ...201121705545
Rapidly Progressive, Fatal, Inhalation Anthraxlike Infection in a Human: Case Report, Pathogen Genome Sequencing, Pathology, and Coordinated Response.Context.-Ten years ago a bioterrorism event involving Bacillus anthracis spores captured the nation's interest, stimulated extensive new research on this pathogen, and heightened concern about illegitimate release of infectious agents. Sporadic reports have described rare, fulminant, and sometimes fatal cases of pneumonia in humans and nonhuman primates caused by strains of Bacillus cereus , a species closely related to Bacillus anthracis . Objectives.-To describe and investigate a case of rapid ...201121827220
molecular characterization of bacillus cereus toxigenic strains isolated from different food matrices in jordan.bacillus cereus is increasingly recognized as one of the causative agents of food poisoning in different parts of the world.201121714637
heat stress leads to superoxide formation in bacillus cereus detected using the fluorescent probe mitosox.bacillus cereus is a food-borne human pathogen and food spoilage organism. spores and vegetative cells of b. cereus can be found almost everywhere and therefore often end up in food processing equipment and food products. to remove spores and vegetative cells from food or equipment, harsh treatments such as high temperatures are applied. the heat stress response of b. cereus and other organisms has been studied and it has been shown that reactive oxygen species may be involved in inactivating th ...201121872960
effect of povidone-iodine concentration and exposure time on bacteria isolated from endophthalmitis cases.to determine the in vitro susceptibility of post-cataract surgery endophthalmitis bacterial isolates to different concentrations of povidone-iodine at different exposure times.201121983301
2,4,6-trinitrophenol degradation by bacillus cereus isolated from a firing range.bacillus cereus strain pu, isolated from soil contaminated with explosive waste, tolerated up to 1.3 mm 2,4,6 trinitrophenol (tnp) and utilize it aerobically as sole nitrogen and carbon source. degradation of tnp was accompanied by stoichiometric release of 2.1 ± 0.15 mol nitrite/mol tnp at 539 μmol/h g dry cell wt. metabolism of tnp was accompanied by transient accumulation of an orange-red metabolite, hydride meisenheimer complex (h-tnp), indicating a metabolic pathway involving complete reduc ...201121858669
removal of hexavalent chromium in tannery wastewater by bacillus cereus.bacillus cereus was used to remove chromium (cr(vi)) from medium containing tannery wastewater under different conditions. the maximum rate of cr(vi) removal was attained at a temperature of 37 °c, ph of 7.0-9.0, and biomass of 20 g/l when the initial cr(vi) concentration was less than 50 mg/l. under the optimum conditions, the cr(vi) in tannery wastewater was treated with each cellular component of b. cereus to detect its ability to reduce cr(vi). the results showed that the removal rate of cr ...201122149215
isolation and identification of soil bacteria growing at the expense of arbuscular mycorrhizal fungi.soil-microorganism symbioses are of fundamental importance for plant adaptation to the environment. research in microbial ecology has revealed that some soil bacteria are associated with arbuscular mycorrhizal fungi (amf). however, these interactions may be much more complex than originally thought. to assess the type of bacteria associated with amf, we initially isolated spores of glomus irregulare from an agrostis stolonifera rhizosphere. the spores were washed with sterile water and plated on ...201121219415
synergistic antibacterial activity of the essential oil of cuminum cyminum l. seed and nisin in a food model.aims:  to investigate effects of various concentrations of the essential oil of cuminum cyminum l. seed alone and in combination with nisin on survival of vegetative forms of bacillus cereus and bacillus subtilis in a food model (commercial barley soup) and their ultrastructure. methods and results:  gas chromatography-mass spectrometry analysis indicated that cumin aldehyde (29·02%) and α-terpinen-7-al (20·70%) constituted the highest amount of the essential oil. the lowest concentration of the ...201121226797
the effects of korean propolis against foodborne pathogens and transmission electron microscopic examination.this study was performed to evaluate the effects of korean propolis against foodborne pathogens and spores of bacillus cereus and to investigate the antimicrobial activity against b. cereus structure by transmission electron microscopy (tem). the antimicrobial effects of the korean propolis were tested against foodborne pathogens including gram-positive (b. cereus, listeria monocytogenes and staphylococcus aureus) and gram-negative (salmonella typhimurium, escherichia coli and pseudomonas fluore ...201121232643
carijodienone from the octocoral carijoa multiflora. a spiropregnane-based steroid.two new steroids (1 and 2) and the known pregna-1,4,20-trien-3-one (3) have been isolated from the pacific octocoral carijoa multiflora. compound 1 possesses a novel spiropregnane-based steroidal skeleton. the photochemical transformation of 3 into 1 allowed the assignment of the absolute configuration at c-10 of 1. the antibacterial activities of compounds 1 and 3 were evaluated against a panel of bacterial strains.201121235217
the effect of lemongrass oil and its major components on clinical isolate mastitis pathogens and their mechanisms of action on staphylococcus aureus dmst 4745.the aims of this study were to investigate the antibacterial activity of lemongrass oil (lg) and its major components which were citral, geraniol and myrcene, against four strains of clinically isolated bovine mastitis pathogens, including staphylococcus aureus, streptococcus agalactiae, bacillus cereus and escherichia coli by the broth microdilution method, as well as their activity on s. aureus biofilm formation. attempts to clarify their mechanisms of action by investigation of the effects on ...201121316719
inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (mangifera indica l.).this study investigated the antimicrobial activities and modes of action of penta-, hexa-, hepta-, octa-, nona-, and deca-o-galloylglucose (gallotannins) isolated from mango kernels. the mics and minimum bactericidal concentrations (mbcs) against food-borne bacteria and fungi were determined using a critical dilution assay. gram-positive bacteria were generally more susceptible to gallotannins than were gram-negative bacteria. the mics of gallotannins against bacillus subtilis, bacillus cereus, ...201121317249
isolation and characterization of cold-active family viii esterases from an arctic soil metagenome.functional screening for lipolytic enzymes at low temperatures resulted in the isolation of the novel cold-active esterases, estm-n1 and estm-n2, from a metagenomic dna library of arctic soil samples. estm-n1 and estm-n2 were 395 and 407 amino acids in length, respectively, and showed the highest similarity to class c β-lactamases. however, they shared a relatively low level of sequence similarity (30%) with each other. phylogenetic analysis of bacterial lipolytic enzymes confirmed that estm-n1 ...201121318360
phenotypic & genetic characterization of bacillus cereus isolated from the acute diarrhoeal patients.bacillus cereus is one of the pathogens responsible for human diarrhoea, mainly due to consumption of contaminated food. the present study was undertaken to determine the occurrence of b. cereus among diarrhoeal patients and its phenotypic and genetic characteristics that determine the virulence and clonal features.201121321425
a flagellin-producing lactococcus strain: interactions with mucin and enteropathogens.bacillus cereus ch is a probiotic strain used in human nutrition whose adhesion to mucin is dependent on its surface-associated flagellin. flagellins from the surface of several probiotic bacillus strains were efficiently extracted with 5 m licl and identified by peptide fingerprinting. based on the proteomic analysis, cloning of the gene coding for the flagellin of b. cereus ch was performed in the lactococcal vector pnz8110 under the control of a nisin-inducible promoter. the resulting strain, ...201121323981
[fatal bacillus cereus sepsis and rhabdomyolysis in a patient with hodgkin's disease]. 201121324561
meta-analysis of endophthalmitis after intravitreal injection of anti-vascular endothelial growth factor agents: causative organisms and possible prevention strategies.: to report the rates of endophthalmitis and the spectrum of causative organisms after intravitreal injection of anti-vascular endothelial growth factor agents and possible prevention strategies.201121330939
current physical and sds extraction methods do not efficiently remove exosporium proteins from bacillus anthracis spores.biochemical studies of the outermost spore layers of the bacillus cereus family are hindered by difficulties in efficient dispersal of the external spore layers and difficulties in dissociating protein complexes that comprise the exosporium layer. detergent and physical methods have been utilized to disrupt the exosporium layer. herein we compare commonly used sds extraction buffers used to extract spore proteins and demonstrate the incomplete extractability of the exosporium layer by these meth ...201121338631
hot water surface pasteurisation of lamb carcasses: microbial effects and cost-benefit considerations.although hot water pasteurisation of carcasses is accepted as a general intervention in usa, this is not the case in europe. the aims of this study were (i) to evaluate the microbiological effects of hot water pasteurisation of lamb carcasses, both after slaughtering and dressing and following subsequent chilling and storage; (ii) to discuss hot water pasteurisation from a public health and cost-benefit perspective; (iii) to discuss the benefits of hot water pasteurisation compared with use of s ...201121356564
comparative analysis of antimicrobial activities of valinomycin and cereulide, the bacillus cereus emetic toxin.cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. cereulide, produced by members of the bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. a comparative analysis of the antimicrobial activity of valinomycin produced by streptomyces spp. and cereulide was performed at a ph range of ph 5.5 to ph 9.5, under anaerobic and aerobic conditions. both compounds display ph-dependent activity agai ...201121357430
pathogen detection using a liquid array technology.low concentrations of microbial pathogens in pure and mixed samples were detected using a bead-based, liquid array technology. a 20-bp sequence in the 23s rrna gene, rrl, was amplified in four microorganisms: bacillus cereus, escherichia coli, salmonella enterica and staphylococcus aureus. pcr products were positively identified with the luminex(®) 100™ system. the system could detect very low amounts of dna and the instrument response was proportional to the input concentration. the lower limit ...201121361936
substrate specificity of spoiiga, a signal-transducing aspartic protease in bacilli.spoiiga is a novel type of membrane-associated aspartic protease that responds to a signal from the forespore by cleaving pro-s(e) in the mother cell during sporulation of bacillus subtilis. very little is known about how spoiiga recognizes pro-s(e). by co-expressing proteins in escherichia coli, it was shown that charge reversal substitutions for acidic residues 24 and 25 of pro-s(e), and for basic residues 245 and 284 of spoiiga, impaired cleavage. these results are consistent with a model pre ...201121362630
multi-parameter flow cytometry and cell sorting reveal extensive physiological heterogeneity in bacillus cereus batch cultures.based on two staining protocols, dioc(6)(3)/propidium iodide (pi) and redoxsensor green (an indicator of bacterial reductase activity)/pi, multi-parameter flow cytometry and cell sorting has identified at least four distinguishable physiological states during batch cultures of bacillus cereus. furthermore, dependent on the position in the growth curve, single cells gave rise to varying numbers of colonies when sorted individually onto nutrient agar plates. these growing colonies derived from a s ...201121365289
osmoadaptative accumulation of n?-acetyl-ß-lysine in green sulfur bacteria and bacillus cereus cect 148t.the compatible solute n(?)-acetyl-ß-lysine (neabl), thus far considered unique to methanogenic archaea, has been found to accumulate in several strains of green sulfur bacteria (gsb) and bacillus cereus cect 148(t) under salt stress. a similar mixture of compatible solutes including trehalose, a-glutamate, ß-glutamate and neabl has been detected in salt-tolerant gsb strains of different phylogenetic branches. the ability of b. cereus to synthesize this compound was predicted from available genom ...201121371089
two capsular polysaccharides enable bacillus cereus g9241 to cause anthrax-like disease.bacillus cereus g9241 causes an anthrax-like respiratory illness in humans; however, the molecular mechanisms of disease pathogenesis are not known. genome sequencing identified two putative virulence plasmids proposed to provide for anthrax toxin (pbcxo1) and/or capsule expression (pbc218). we report here that b. cereus g9241 causes anthrax-like disease in immune-competent mice, which is dependent on each of the two virulence plasmids. pbcxo1 encodes paga1, the homologue of anthrax protective a ...201121371137
comparing the mannitol-egg yolk-polymyxin agar plating method with the three-tube most-probable-number method for enumeration of bacillus cereus spores in raw and high-temperature, short-time pasteurized milk.the u.s. food and drug administration's bacteriological analytical manual recommends two enumeration methods for bacillus cereus: (i) standard plate count method with mannitol-egg yolk-polymyxin (myp) agar and (ii) a most-probable-number (mpn) method with tryptic soy broth (tsb) supplemented with 0.1% polymyxin sulfate. this study compared the effectiveness of myp and mpn methods for detecting and enumerating b. cereus in raw and high-temperature, short-time pasteurized skim (0.5%), 2%, and whol ...201121375885
effectiveness of chitosan against mature biofilms formed by food related bacteria.chitosan has proven antimicrobial properties against planktonic cell growth. little is known, however, about its effects on already established biofilms. oriented for application in food industry disinfection, the effectiveness of both medium molecular weight (mmw) chitosan and its enzymatically hydrolyzed product was tested against mature biofilms of four pathogenic strains, listeria monocytogenes, bacillus cereus, staphylococcus aureus and salmonella enterica, and a food spoilage species, pseu ...201121340015
synthesis and structure of [(?(6)-p-cymene)ru(2-anthracen-9-ylmethylene-n-ethylhydrazinecarbothioamide)cl]cl; biological evaluation, topoisomerase ii inhibition and reaction with dna and human serum albumin.we have synthesized and evaluated the biological properties of a compound of the type [?(6)-p-cymene)ru(etatsc)cl]cl (1) where etatsc = 2-anthracen-9-ylmethylene-n-ethylhydrazinecarbothioamide, a thiosemicarbazone. the complex has been characterized by elemental analysis, spectroscopically (nmr, uv-vis, and ir) and structurally by xrd. the in vitro anticancer activity of 1 has been evaluated against two human colon cancer cell lines. the ic(50) value for activity against hct-116 was 224 ± 7 µm a ...201121347491
characterization of a novel temperate phage originating from a cereulide-producing bacillus cereus strain.a novel temperate bacteriophage was isolated from a bacillus cereus cereulide-producing strain and named vb_bces-iebh. vb_bces-iebh belongs to the siphoviridae family. the complete genome sequence (53 kb) was determined and annotated. eighty-seven orfs were detected and for 28, a putative function was assigned using the aclame database. vb_bces-iebh replicates as a plasmid in the prophage state. accordingly, a 9-kb plasmid-like region composed of 13 orfs was identified. a fragment of around 2000 ...201121349326
effect of olive powder and high hydrostatic pressure on the inactivation of bacillus cereus spores in a reference medium.the aim of this work was to study the effect of olive powder combined with high hydrostatic pressure (hhp) on the inactivation of bacillus cereus spores, to use it as an additional control hurdle in beverages pasteurised by this technology. with this purpose, reference medium prepared at different concentrations of olive powder was inoculated with b. cereus spores and subjected to different pressure treatments. the outgrowth capacity of the treated spores was then determined at 20-¦c and 32-¦c. ...201121381901
emetic disease caused by bacillus cereus after consumption of tuna fish in a beach club.several vomiting episodes were reported in december 2007 by the management of a beach club in calvi+á. the subsequent case investigation confirmed tuna fish consumption a few hours before onset of emesis in all cases. microbiological analyses detected high bacterial levels in ready-to-eat fish samples, indicating inappropriate cooking procedures. more important, elevated levels of bacillus cereus were present both in raw and cooked fish. no other pathogens were detected, indicating b. cereus as ...201121381943
maklamicin, an antibacterial polyketide from an endophytic micromonospora sp.a new spirotetronate-class polyketide, maklamicin (1), was isolated from the culture extract of an endophytic actinomycete of the genus micromonospora. the structure and relative configuration of 1 were elucidated by interpretation of nmr and other spectroscopic data, and the absolute configuration was determined using the modified mosher method. maklamicin (1) showed strong to modest antimicrobial activity against gram-positive bacteria.201121388191
partial characterization of bacteriocins produced by two lactobacilus strains with probiotic properties.the probiotic characteristics of lactobacillus brevis bg18 and lb. plantarum bg33, isolated from traditional turkish tulum cheese were assessed. these two bacteriocinproducer strains exhibited good probiotic characteristics such as resistance in media containing 0.3% bile salt, pepsin (3 mg mlôü+-¦), and pancreatine (1 mg mlôü+-¦) as well as acid resistance at ph 2. they were also adhered to caco-2 epithelial cells in a manner comparable to escherichia coli lmg3083 (etec) and salmonella typhimur ...201121388923
improving the ethanol yield by reducing glycerol formation using cofactor regulation in saccharomyces cerevisiae.to increase ethanol yield and decrease glycerol production in saccharomyces cerevisiae, the strategies of direct cofactor-regulation were explored. during anaerobic batch fermentations, the yeast expressing bacillus cereus gapn gene, encoding non-phosphorylating nadp(+)-dependent glyceraldehyde-3-phosphate dehydrognease, produced 73.8 g ethanol l(-1), corresponding to 96% of theoretical maximum yield compared to 92% for the wild type. the yeast expressing escherichia coli frda gene encoding the ...201121400237
toxin genes profiles and toxin production ability of bacillus cereus isolated from clinical and food samples.bacillus cereus can cause diarrheal and emetic type of food poisoning but little study has been done on the main toxins of food poisoning caused by b. cereus in korea. the objective of this study is to characterize the toxin gene profiles and toxin-producing ability of 120 b. cereus isolates from clinical and food samples in korea. the detection rate of nheabc, hblcda, entfm, and cytk enterotoxin gene among all b. cereus strains was 94.2, 90.0, 65.8, and 52.5%, respectively. the ces gene encodin ...201121535727
gastrointestinal passage of bacillus cereus. 201121539184
synthesis and antimicrobial activities of hexahydroimidazo[1,5-a]pyridinium bromides with varying benzyl substituents.variously substituted benzyl bromides were employed to quaternize hexahydrobenzylimidazo[1,5-a]pyridine (a) and the resulting bromides (1-11) were evaluated for their in vitro antimicrobial activity against 10 pathogenic microorganisms: staphylococcus aureus, staphylococcus epidermidis, bacillus cereus, micrococcus luteus, proteus vulgaris, escherichia coli, salmonella typhimurium, klebsiella pneumonia, candida albicans and candida krusei. antimicrobial activities were surprisingly high (mic: 0. ...201121543140
growth inhibition and induction of stress protein, groel, of bacillus cereus exposed to antibacterial peptide isolated from bacillus subtilis sc-8.this study was conducted to investigate the antibacterial effect of bsap-254 on bacillus cereus with the induced stress proteins. the bsap-254 is an antimicrobial peptide isolated from soybean-fermenting bacteria, bacillus subtilis sc-8. it had a narrow spectrum of activity against b. cereus group. the growth inhibitory effect of bsap-254 (50 μg/ml) reduced the population of b. cereus from >10(8) to 10(4) colony-forming units per milliliter within 30 min. in b. cereus exposed to bsap-254, 14 int ...201121544555
occurrence of microorganisms of public health and spoilage significance in fruit juices sold in retail markets in greece.fruit juices are an important part of the modern diet in many countries. however, few data are available concerning the microbiological quality of the fruit juices sold in greece. using standard microbiological procedures, we conducted a bacteriological survey of commercially sold, pasteurized, shelf-stable fruit juices from retail markets. a total of 120 samples of fruit juices sold in various retail markets were examined for their bacteriological quality. the ph of the tested juices was 2.4-4. ...201121545842
production and properties of two collagenases from bacteria and their application for collagen extraction.two collagenolytic protease (collagenase) producing bacteria, a gram positive bacillus cereus cna1 and a gram negative klebsiella pneumoniae cnl3, were isolated under alkaline and acidic conditions, respectively. the production of collagenase by these two bacteria was optimized. glycerol was the suitable carbon source for collagenase production by both strains. the optimal initial ph values for collagenase production by cna1 and cnl3 were 7.5 and 6.0, respectively, and the optimal temperature wa ...201121549225
potential application of the nisin z preparation of lactococcus lactis w8 in preservation of milk.aims:  the aim of the study is to evaluate the effectiveness of the preparation of nisin z from lactococcus lactis w8-fermented milk in controlling the growth of spoilage bacteria in pasteurized milk. methods and results:  spoilage bacteria isolated from pasteurized milk at 8 and 15°c were identified as enterococcus italicus, enterococcus mundtii, enterococcus faecalis, bacillus thuringiensis, bacillus cereus, lactobacillus paracasei, acinetobacter sp., pseudomonas fluorescens and enterobacter a ...201121554339
bacillus cereus responses to acid stress.coping with acid environments is one of the prerequisites for the soil saprophytic and human pathogenic lifestyle of bacillus cereus. this minireview highlights novel insights in the responses displayed by vegetative cells and germinating spores of b. cereus upon exposure to low ph as well as organic acids, including acetic acid, lactic acid and sorbic acid. insights regarding the possible acid-inflicted damage, physiological responses and protective mechanisms have been compiled based on single ...201121554514
antibacterial peptides derived from caprine whey proteins, by digestion with human gastrointestinal juice.peptides in caprine whey were identified after in vitro digestion with human gastrointestinal enzymes in order to determine their antibacterial effect. the digestion was performed in two continuing steps using human gastric juice (ph 2·5) and human duodenal juice (ph 8) at 37°c. after digestion the hydrolysate was fractionated and 106 peptides were identified. from these results, twenty-two peptides, located in the protein molecules, were synthesised and antibacterial activity examined. strong a ...201121554806
antioxidant, antihypertensive and antimicrobial properties of ovine milk caseinate hydrolyzed with a microbial protease.background: bioactive peptides might be released from precursor proteins through enzymatic hydrolysis. these molecules could be potentially employed in health and food products. in this investigation, ovine milk caseinate hydrolysates obtained with a novel microbial protease derived from bacillus sp. p7 were evaluated for antioxidant, antimicrobial, and angiotensin i-converting enzyme (ace)-inhibitory activities. results: antioxidant activity measured by the 2,2'-azino-bis-(3-ethylbenzothiazolin ...201121560133
characterization of bacilli isolated from the confined environments of the antarctic concordia station and the international space station.abstract bacillus and related genera comprise opportunist and pathogen species that can threaten the health of a crew in confined stations required for long-term missions. in this study, 43 bacilli from confined environments, that is, the antarctic concordia station and the international space station, were characterized in terms of virulence and plasmid exchange potentials. no specific virulence feature, such as the production of toxins or unusual antibiotic resistance, was detected. most of th ...201121563959
follow-up of the bacillus cereus emetic toxin production in penne pasta under household conditions using liquid chromatography coupled with mass spectrometry.two outbreak-related bacillus cereus emetic strains were investigated for their growth and cereulide production potential in penne pasta at 4, 8 and 25 °c during 7-day storage. cereulide production was detected and quantified by lc-ms method (lod of 1 ng/ml, loq of 5 ng/ml) and growth was determined by culture-based enumeration. inoculated b. cereus strains (10(5) cfu/g) were able to reach counts of more than 10(8) cfu/g and cereulide production of about 500 ng/g already after 3 days of storage ...201121569959
isolation of glucosidase and phospholipase positive bacillus circulans on aloa medium.aim:  to report the growth of glucosidase and phospholipase positive bacteria on agar listeria according to ottaviani and agosti (aloa) different from listeria monocytogenes, listeria ivanovii and bacillus cereus. methods and results:  raw water-buffalo milk was analysed according to en iso 11290. streaking of fraser broth on aloa resulted in green colonies with an opaque halo after 48 h at 30°c. colonies were transferred onto tryptone soya yeast extract agar and showed cultural characteristics ...201121575023
bacillus cereus g9241 makes anthrax toxin and capsule like highly virulent b. anthracis ames but behaves like attenuated toxigenic nonencapsulated b. anthracis sterne in rabbits and mice.bacillus cereus g9241 was isolated from a welder with a pulmonary anthrax-like illness. the organism contains two megaplasmids, pbcxo1 and pbc218. these plasmids are analogous to the b. anthracis ames plasmids pxo1 and pxo2 that encode anthrax toxins and capsule, respectively. here we evaluated the virulence of b. cereus g9241 as well as the contributions of pbcxo1 and pbc218 to virulence. b. cereus g9241 was avirulent in new zealand rabbits after subcutaneous inoculation and attenuated 100-fold ...201121576337
successful surgical drainage and aggressive medical therapy in a preterm neonate with bacillus cereus meningitis.bacillus cereus meningitis is a rare disease with a very high mortality rate in neonates. the authors present the rare case of a premature infant with b. cereus bacteremia and subsequent intracranial abscesses. in addition to aggressive medical therapy, surgical drainage was performed via a left frontal mini-craniotomy. at 15 months of age, the patient had mild developmental delay, cortical blindness, and sensorineural hearing loss. the clinical case is described and difficulties in the manageme ...201121577046
physiological parameters of bacillus cereus marking the end of acid-induced lag phases.during lag phases microbial cells adapt to their environment and prepare to proliferate. physiological parameters of b. cereus cells upon exposure to near-growth-boundary acid stress were investigated and markers for the transition between lag phase and growth were identified using fluorescent probes combined with flow cytometry. determination of cell counts and optical density revealed lag phases of 1h, 2h and 5h, in cultures shifted to ph 7, ph 5.3 (set with lactic acid) and ph 4.9 (set with s ...201121592605
synthesis of gold and silver nanoparticles using purified urak.this study aims at developing a new eco-friendly process for the synthesis of silver nanoparticles (agnps) and gold nanoparticles (aunps) using purified urak. urak is a fibrinolytic enzyme produced by bacillus cereus nk1. the enzyme was purified and used for the synthesis of aunps and agnps. the enzyme produced agnps when incubated with 1mm agno3 for 24h and aunps when incubated with 1mm haucl4 for 60h. but when naoh was added, the synthesis was rapid and occurred within 5min for agnps and 12h f ...201121592748
sulfamethoxazole biodegradation and biotransformation in the water-sediment system of a natural river.in this study, the biodegradation of sulfamethoxazole (smx) as affected by temperature, humic acid (ha) and smx concentrations was investigated by hplc-ms/ms analysis based on water-sediment batch experiments. the first order decay model (c=c(0)×exp (-kt)) was best fitted for smx biodegradation. smx degradation significantly increased with elevated temperature (degradation rate was 82.9% at 25°c vs. 40.5% at 4°c in sediment), ha contents (30mg/l of ha facilitated smx degradation rate at 90.1% vs ...201121596556
monitoring the wet-heat inactivation dynamics of single spores of bacillus species using raman tweezers, differential interference contrast and nucleic acid dye fluorescence microscopy.dynamic processes during wet heat treatment at 80-90°c of individual spores of bacillus cereus, bacillus megaterium and bacillus subtilis were investigated using dual-trap raman spectroscopy, differential interference contrast (dic) microscopy and nucleic acid stain (syto 16) fluorescence microscopy. during spore wet heat treatment, while spores' 1:1 chelate of ca(2+) with dipicolinic acid (cadpa) was released rapidly at a highly variable time t(lag), the levels of spore nucleic acids remained n ...201121602365
enzymatic synthesis of isopropyl acetate by immobilized bacillus cereus lipase in organic medium.selective production of fragrance fatty acid ester from isopropanol and acetic acid has been achieved using silica-immobilized lipase of bacillus cereus mtcc 8372. a purified thermoalkalophilic extracellular lipase was immobilized by adsorption onto the silica. the effects of various parameters like molar ratio of substrates (isopropanol and acetic acid; 25 to 100 mm), concentration of biocatalyst (25-125 mg/ml), reaction time, reaction temperature, organic solvents, molecular sieves, and initia ...201121603222
screening of australian plants for antimicrobial activity against campylobacter jejuni.campylobacter jejuni is the most common cause of acute enteritis in humans, with symptoms such as diarrhoea, fever and abdominal cramps. in this study, 115 extracts from 109 australian plant species were investigated for their antimicrobial activities against two c. jejuni strains using an in vitro broth microdilution assay. among the plants tested, 107 (93%) extracts showed activity at a concentration between 32 and 1024 µg/ml against at least one c. jejuni strain. seventeen plant extracts were ...201121604309
[screening endophytic bacteria against plant-parasitic nematodes].plant-parasite nematode is one of the most important pathogens in plant. our objective is to screen endophytic bacteria against plant-parasitic nematodes from plant.201121604551
antibacterial activity of chemically defined chitosans: influence of molecular weight, degree of acetylation and test organism.chitosans, polysaccharides obtained from the exoskeleton of crustaceans, have been shown to exert antibacterial activity in vitro and their use as a food preservative is of growing interest. however, beyond a consensus that chitosan appears to disrupt the bacterial cell membrane, published data are inconsistent on the chemical characteristics that confer the antibacterial activity of chitosan. while most authors agree that the net charge density of the polymer (reflected in the fraction of posit ...201121605923
efficacy of vitrectomy in improving the outcome of bacillus cereus endophthalmitis.purpose:: to evaluate the efficacy of vitrectomy with vancomycin for the treatment of experimental bacillus cereus endophthalmitis. methods:: endophthalmitis was initiated in rabbits via intravitreal injection of 100 colony-forming unit b. cereus. treatment groups included 25-gauge transconjunctival sutureless vitrectomy with intravitreal vancomycin (1 mg) or vancomycin alone. groups were treated at 4, 5, or 6 hours after infection. at 48 hours (for 4-hour and 5-hour groups) or 36 hours (for the ...201121606892
mutually exclusive distribution of the sap and eag s-layer genes and the lytb/lyta cell wall hydrolase genes in bacillus thuringiensis.recently, two bacillus thuringiensis strains were reported to synthesize parasporal inclusion bodies made not of the expected crystal (cry) proteins but rather of the surface layer proteins (slp) sap (encoded by sap) and ea1 (encoded by eag), respectively. whether the presence of the sap and eag genes is restricted to these two b. thuringiensis strains or ubiquitous in b. thuringiensis is unknown. we report here the distribution of the sap and eag genes in b. thuringiensis. strains in the bacill ...201121611767
bacterial load of fresh vegetables and their resistance to the currently used antibiotics in saudi arabia.abstract this study was carried out to describe the bacterial load and the occurrence of some disease-causing enteric bacteria on raw vegetables sold in saudi markets. the study further aimed to analyze antibiotic resistance rates, production of extended-spectrum beta lactamase, and plasmid carriage among bacterial population of raw vegetables. results revealed that none of them contained bacillus cereus, salmonella, and escherichia coli o157:h7. however, staphylococcus aureus and shigella were ...201121612423
antifungal quinazolinones from marine-derived bacillus cereus and their preparation.two new quinazolinones alkaloids, r(+)-2-(heptan-3-yl)quinazolin-4(3h)-one (1) and (2r,3'r)+(2s,3'r)-2-(heptan-3-yl)-2,3-dihydroquinazolin-4(1h)-one (2) (a pair of epimers), as well as seven known analogues, 2-methylquinazolin-4(3h)-one (3), 2-benzylquinazolin-4(3h)-one (4), cyclo-(pro-ile), cyclo-(pro-leu), cyclo-(pro-val), cyclo-(pro-phe), and cyclo-(tyr-pro) were isolated from the n-butyl alcohol extract of the marine-derived bacterium bacillus cereus 041381. the new compounds were identified ...201121612927
some like it hot: the effects of climate change on reproduction, immune function and disease resistance in the cricket gryllus texensis.in many parts of the world, climate change is increasing the frequency and severity of heat waves. how do heat waves impact short-lived poikilotherms such as insects? in the cricket, gryllus texensis, 6 days of elevated temperatures (i.e. 7°c above the average field temperature and 5°c above their preferred temperature) resulted in increased egg laying, faster egg development and greater mass gain. the increased temperature also increased activity of phenoloxidase and lysozyme-like enzymes, two ...201121613515
natural resistance of rose petals to microbial attack.petals of red, yellow and white roses (rosa damascene mill.) of the family rosaceae were extracted with (1:1) methylene chloride/methanol and tested for their antimicrobial activities against four species of gram-positive bacteria (bacillus cereus, bacillus subtilis, micrococcus luteus and staphylococcus aureus), five species of gram-negative bacteria (enterobacter aerogenes, escherichia coli, klebsiella pneumonia, pseudomonas aeruginosa and serratia marcescens) and five species of fungi (penici ...201121614712
preparation of mucoadhesive oral patches containing tetracycline hydrochloride and carvacrol for treatment of local mouth bacterial infections and candidiasis.the specific aim of this work was to prepare mucoadhesive patches containing tetracycline hydrochloride and carvacrol in an attempt to develop a novel oral drug delivery system for the treatment of mouth infections. the bilayered patches were prepared using ethyl cellulose as a backing layer and carbopol 934 as a matrix mucoadhesive layer. patches were prepared with different loading amounts of tetracycline hydrochloride and carvacrol. the antimicrobial activity was assessed for the prepared pat ...201121617783
molecular weight change of polyhydroxyalkanoate (pha) caused by the phac subunit of pha synthase from bacillus cereus yb-4 in recombinant escherichia coli.polyhydroxyalkanoate (pha)-producing bacillus strains possess class iv pha synthases composed of two subunit types, namely, phar and phac. in the present study, pha synthases from bacillus megaterium nbrc15308(t) (pharc(bm)), b. cereus yb-4 (pharc(yb4)), and hybrids (phar(bm)c(yb4) and phar(yb4)c(bm)) were expressed in escherichia coli jm109 to characterize the molecular weight of the synthesized poly(3-hydroxybutyrate) [p(3hb)]. pharc(bm) synthesized p(3hb) with a relatively high molecular weig ...201121618968
reference genes for quantitative, reverse-transcription pcr in bacillus cereus group strains throughout the bacterial life cycle.quantitative reverse-transcription pcr (rt-qpcr) has become a major tool to better understand the biology and pathogenesis of bacteria. one prerequisite of valid rt-qpcr data is their proper normalization to stably expressed reference genes. to identify and evaluate reference genes suitable for normalization of gene expression data in bacillus cereus group strains, mrna levels of eleven candidate reference genes (rpsu, nifu, udp (udp-n-acetylglucosamine 2-epimerase), bt9727_5154/bc_5475, bt9727_ ...201121620905
role played by exosporium glycoproteins in the surface properties of bacillus cereus spores and in their adhesion properties to stainless steel.b. cereus spores are surrounded by a loose fitting layer, called the exosporium, whose distal part is mainly formed of glycoproteins. the role played by the exosporium glycoproteins of b. cereus atcc 14579 (bcla, exsh) was investigated considering hydrophobicity and charge, as well as adhesion properties of spores to stainless steel. the absence of bcla increased both the isoelectric point (iep) and hydrophobicity of whole spores, whilst simultaneously reducing the interaction between spores and ...201121622795
phytochemical analysis and antimicrobial activities of methanolic extracts of leaf, stem and root from different varieties of labisa pumila benth.a local herb, kacip fatimah, is famous amongst malay women for its uses in parturition; however, its phytochemical contents have not been fully documented. therefore, a study was performed to evaluate the phenolics, flavonoids, and total saponin contents, and antibacterial and antifungal properties of the leaf, stem and root of three varieties of labisia pumila benth. total saponins were found to be higher in the leaves of all three varieties, compared to the roots and stems. leaves of var. pumi ...201121623314
examining the genetic variation of reference microbial cultures used within food and environmental laboratories using fluorescent amplified fragment length polymorphism analysis.fluorescent amplified fragment length polymorphism (faflp) analysis was applied to genetically fingerprint 'working culture control strains' used by accredited food microbiology laboratories. a working culture control strain is defined as a sub-culture from a strain initially obtained from an authenticated source (such as the national collection of type cultures, nctc), that is maintained for use with routine testing within the laboratory. working culture control strains from eight food examinat ...201121623896
automated spectral smoothing with spatially adaptive penalized least squares.a variety of data smoothing techniques exist to address the issue of noise in spectroscopic data. the vast majority, however, require parameter specification by a knowledgeable user, which is typically accomplished by trial and error. in most situations, optimized parameters represent a compromise between noise reduction and signal preservation. in this work, we demonstrate a nonparametric regression approach to spectral smoothing using a spatially adaptive penalized least squares (sapls) approa ...201121639989
bacillus anthracis: molecular taxonomy, population genetics, phylogeny and patho-evolution.bacillus anthracis, the etiological agent of anthrax, manifests a particular bimodal lifestyle. this bacterial species alternates between short replication phases of 20-40 generations that strictly require infection of the host, normally causing death, interrupted by relatively long, mostly dormant phases as spores in the environment. hence, the b. anthracis genome is highly homogeneous. this feature and the fact that strains from nearly all parts of the world have been analysed for canonical si ...201121640849
global gene expression profile of swarming bacillus cereus.bacillus cereus can use swarming to move over and colonize solid surfaces in different environments. this kind of motility is a collective behavior accompanied by the production of long and hyperflagellate swarm cells. in this study, the genome-wide transcriptional response of b. cereus atcc 14579 during swarming was analyzed. swarming was shown to trigger the differential expression (>2 fold change) of 118 genes. down-regulated genes included those required for basic cellular metabolism. in acc ...201121642396
lysozyme as a barrier to growth of bacillus anthracis strain sterne in liquid egg white, milk and beef.in this study, we investigated the role of lysozyme on the viability of bacillus cereus, bacillus subtilis, bacillus pumilus and bacillus anthracis (sterne) in egg white (ew), ground beef and milk. at 35 °c in ew, growth rates (gr) for b. cereus, b. subtilis, b. pumilus and b. anthracis were 0.005, -0.018, -0.028 and -0.029 od(600)/h, respectively. heat-treating ew at 55 and 60 °c reduced the inactivating effect of ew by 3.1 and 10.5-fold, respectively. addition of lysozyme (2 mg/ml) to 60 °c-tr ...201121645824
aerobic degradation of 2,4-dinitrotoluene by individual bacterial strains and defined mixed population in submerged cultures.the degradation efficiencies of isomeric mononitrotoluenes (2- and 4-nts) and dinitrotoluenes (2,4-dnt and 2,6-dnt) by either individual bacterial strains (bacillus cereus ndt4, pseudomonas putida ndt1, pseudomonas fluorescens ndt2, and achromobacter sp. ndt3) or their mixture were compared in submerged batch cultivations. the mixed culture degraded 2,4-dnt nearly 50 times faster than any of the individual strains. the mixed culture also demonstrated significantly shorter lag periods in 2,4-dnt ...201121665364
anti-proliferative activity of meroditerpenoids isolated from the brown alga stypopodium flabelliforme against several cancer cell lines.the sea constitutes one of the most promising sources of novel compounds with potential application in human therapeutics. in particular, algae have proved to be an interesting source of new bioactive compounds. in this work, six meroditerpenoids (epitaondiol, epitaondiol diacetate, epitaondiol monoacetate, stypotriol triacetate, 14-ketostypodiol diacetate and stypodiol) isolated from the brown alga stypopodium flabelliforme were tested for their cell proliferation inhibitory activity in five ce ...201121673894
prevalence of bacillus cereus bacteriophages in fermented foods and characterization of phage jbp901.bacillus cereus is widely distributed in the environment and is responsible for foodborne illnesses, causing diarrhea and vomiting. in this study, the prevalence of b. cereus bacteriophages in korean fermented foods was studied using 6 reference strains, and jbp901 was further characterized. in a total of 47 samples, 19 (40.4%) contained more than one kind of b. cereus phage. fourteen phages were purified and determined for host lysis profile. among them, jbp901 was specific to b. cereus group s ...201121810470
microbial control of food-related surfaces: na-chlorophyllin-based photosensitization.the aim of this study was to evaluate efficiency of photosensitization as surface sanitation alternative using model systems when food pathogens, their spores and biofilms were attached to the food-related surface (polyolefine). in addition it was important to compare antibacterial efficiency of na-chlorophyllin (na-chl)-based photosensitization with conventional sanitizers. obtained results indicate that bacilluscereus atcc 12826 and listeriamonocytogenes atcc 7644 as well as their thermoresist ...201121807530
antimicrobial activity of mangrove plant (lumnitzera littorea).to investigate the antimicrobial activities of n-hexane, ethyl acetate and methanol extracts of the leaves of lumnitzera littorea (l. littorea) against six human pathogenic microbes.201121803301
structural analysis of papain-like nlpc/p60 superfamily enzymes with a circularly permuted topology reveals potential lipid binding sites.nlpc/p60 superfamily papain-like enzymes play important roles in all kingdoms of life. two members of this superfamily, lrat-like and yaef/yiix-like families, were predicted to contain a catalytic domain that is circularly permuted such that the catalytic cysteine is located near the c-terminus, instead of at the n-terminus. these permuted enzymes are widespread in virus, pathogenic bacteria, and eukaryotes. we determined the crystal structure of a member of the yaef/yiix-like family from bacill ...201121799766
inhibition of bacillus cereus spore outgrowth and multiplication by chitosan.bacillus cereus is an endospore-forming bacterium able to cause food-associated illness. different treatment processes are used in the food industry to reduce the number of spores and thereby the potential of foodborne disease. chitosan is a polysaccharide with well-documented antibacterial activity towards vegetative cells. the activity against bacterial spores, spore germination and subsequent outgrowth and growth (the latter two events hereafter denoted (out)growth), however, is poorly docume ...201121798612
[isolation, identification and characterization of cypermethrin-degrading strain l12].to isolate and characterize bacteria to degrade cypermethrin (cp).201121796986
in vitro antimicrobial and antioxidant activity of acetone and methanol extracts from thymus leucotrichius (lamiaceae).thymus species has been used as tonic and herbal tea, antiseptic, antitussive, carminative, antioxidant, anti-inflammatory and antimicrobial activities. the acetone and methanol extracts of thymus (t.) leucotrichius (labiatae/lamiaceae) was examined for antimicrobial and antioxidant properties.201121796869
synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents.a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ...201121794959
design and synthesis of novel antimicrobial acyclic and heterocyclic dyes and their precursors for dyeing and/or textile finishing based on 2-n-acylamino-4,5,6,7-tetrahydro-benzo[b]thiophene systems.a series of novel polyfunctionalized acyclic and heterocyclic dye precursors and their respective azo (hydrazone) counterpart dyes and dye precursors based on conjugate enaminones and/or enaminonitrile moieties were synthesized. the dyes and their precursors are based on 2-cyano-n-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-acetamide, 2-ethoxycarbonyl-n-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-acetamide or 2-phenylcarbamoyl-n-(3-cyano-4,5,6,7-tetrahydrobenzo[b]thiophen-2-yl)-aceta ...201121792147
effects of photoactivated titanium dioxide nanopowders and coating on planktonic and biofilm growth of pseudomonas aeruginosa.we exploited the ability of photocatalytic titanium dioxide (tio(2) ) as an agent for the biofilm control. two photocatalytic systems were investigated: a 3g/l suspension of tio(2) nanopowder in demineralised water and glass slides coated with a tio(2) thin film, achieved by sol-gel deposition. a running protocol for the photoactivation of tio(2) was set up using the dye rhodamine b. the microorganisms studied were pseudomonas stutzeri, pseudomonas aeruginosa and a bacillus cereus-group as plank ...201121790617
purification and characterization of an endoxylanase from the culture broth of bacillus cereus bsa1.an extracellular xylanase from the fermented broth of bacillus cereus bsa1 was purified and characterized. the enzyme was purified to 3.43 fold through ammonium sulphate precipitation, deae-cellulose chromatography and followed by gel filtration through sephadex g-100 column. the molecular mass of the purified xylanse was about 33 kda. the enzyme was an endoxylanase as it initially degraded xylan to xylooligomers. the purified enzyme showed optimum activity at 55 degrees c and at ph 7.0 and rema ...201121790026
environmental and biofilm-dependent changes in a bacillus cereus secondary cell wall polysaccharide.bacterial species from the bacillus genus, including bacillus cereus and bacillus anthracis, synthesize secondary cell wall polymers (scwp) covalently associated to the peptidoglycan through a phospho-diester linkage. although such components were observed in a wide panel of b. cereus and b. anthracis strains, the effect of culture conditions or of bacterial growth state on their synthesis has never been addressed. herein we show that b. cereus atcc 14579 can synthesize not only one, as previous ...201121784857
narrow antagonistic activity of antimicrobial peptide from bacillus subtilis sck-2 against bacillus cereus.bacillus subtilis sck-2, producing an antimicrobial peptide of this study, was isolated from kyeopjang, the korean traditional fermented-soybean paste. this strain showed a narrow antagonistic activity as it inhibited bacillus cereus causing food poisoning in human. the antimicrobial peptide, tentatively named amp ic-1, was purified, characterized, and compared to bsap-254, another peptide which was previously recovered from traditionally fermented-soybean paste. amp ic-1 was found to be more th ...201121783410
domains of bcla, the major surface glycoprotein of the b. cereus exosporium: glycosylation patterns and role in spore surface properties.the role of the bcla domains of b. cereus atcc 14579 was investigated in order to understand the phenomena involved in the interfacial processes occurring between spores and inert surfaces. this was done by (i) creating deletions in the collagen-like region (clr) and the c-terminal domain (ctd) of bcla, (ii) building bcla proteins with various lengths in the clr and (iii) modifying the hydrophobic upper surface in the ctd. first, it was demonstrated that the clr was substituted by three residues ...201121777114
the analysis of the immobilization mechanism of ni(ii) on bacillus cereus.this work focused on the identification of biosorption mechanism of ni(ii) by living bacillus cereus (b. cereus) based on batch experiments and a variety of microscopic equipments. the adsorption equilibrium reached rapidly in 2 h and the maximum nickel adsorption capability of b. cereus was 17.7 mg x g(-1) (dry weight). atomic force microscopy (afm) analysis showed that the bacterial surface roughness increased from 7.9 +/- 0.5 nm to 12.6 +/- 1.6 nm during this process. scanning electron micros ...201121776742
l-asparaginase production by mangrove derived bacillus cereus mab5: optimization by response surface methodology.to isolate marine bacteria, statistically optimize them for maximum asparaginase production.201121771705
evaluation of four microbial class ii fructose 1,6-bisphosphate aldolase enzymes for use as biocatalysts.fructose 1,6-bisphosphate (fbp) aldolase has been used as biocatalyst in the synthesis of several pharmaceutical compounds such as monosaccharides and analogs. is has been suggested that microbial metal-dependant class ii aldolases could be better industrial catalysts than mammalian class i enzyme because of their greater stability. the class ii aldolases from four microbes were subcloned into the escherichia coli vector pt7-7, expressed and purified to near homogeneity. the kinetic parameters, ...201121763425
Refresh
firstprev
...21222324252627282930...
nextlast
Displaying items 2001 - 2100 of 8187