Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| highly sensitive detection of dna phosphorylation by counting single nanoparticles. | dna phosphorylation is a vital process in the repair, replication, and recombination of nucleic acids. traditionally, it is assayed by denaturing gel electrophoresis and autoradiography, which are tedious and not sensitive. we report on the development of a sensitive, simple, and economical method for dna phosphorylation detection and t4 polynucleotide kinase (t4 pnk) activity assay based on marking dna phosphorylation/biotinylation events by the attachment of fluorescent nanoparticles. enzyme a ... | 2010 | 20512316 |
| squamous cell carcinoma of the temporal bone. | to study the survival outcomes of patients with squamous cell carcinoma (scc) of the temporal bone. a secondary purpose was to evaluate the university of pittsburgh staging system as a predictor of survival. | 2010 | 20513031 |
| integrated positron emission tomography-computed tomography does not accurately stage intrathoracic disease of patients undergoing trimodality therapy for malignant pleural mesothelioma. | the results of trimodality therapy for malignant pleural mesothelioma (mpm) are related to stage. staging can be carried out by computed tomography (ct), magnetic resonance imaging and fluorodeoxyglucose positron emission tomography (fdg-pet), but all modalities suffer from limitations. we investigated whether integrated pet-ct improved the intrathoracic staging of patients undergoing trimodality therapy for mpm. | 2010 | 20514576 |
| effect of intrapleural povidone-iodine lavage on thyroid hormones in thoracic surgery. | povidone-iodine (pi) is an effective disinfectant used in many surgical operations. it is known that pi is absorbed by the skin after application. absorption is even greater in infants, as their skin is thinner and more permeable. iodine absorption in the orbita, neck and during abdominal surgery has been previously investigated, but such a study has not been performed for the pleura, which has a greater area and capacity for absorption. in this study, we investigated the effect of intraoperativ ... | 2010 | 20514578 |
| menstrual irregularity: a possible clinical marker of metabolic dysfunction in women with class iii obesity. | to evaluate whether menstrual irregularity in morbidly obese women is indicative of metabolic dysfunction. | 2010 | 20515256 |
| phase ii study of pre-operative radiotherapy with capecitabine and oxaliplatin for rectal cancer and carcinoembryonic antigen as a predictor of pathological tumour response. | this study investigated the efficacy and tolerability of pre-operative radiotherapy with concurrent capecitabine and oxaliplatin in patients with rectal cancer. forty-seven patients with rectal adenocarcinoma (stages t3 - t4; node-positive) were enrolled and received radiotherapy (46 gy in 23 fractions) in combination with capecitabine (1000 mg/m(2) twice daily on days 1 - 14 and 22 - 35) and oxaliplatin (130 mg/m(2) on days 1 and 22) (xelox regimen). the main endpoints were safety and efficacy, ... | 2010 | 20515578 |
| subclinical thyroid disease in elderly subjects. | subclinical thyroid disease (std) is defined as circulating concentrations of free t4 and free t3 within their respective reference ranges in the presence of abnormal circulating concentrations of tsh. scd is being diagnosed more frequently in clinical practice and is reported to be more prevalent in elderly as compared to young or adult subjects. the clinical impact of subclinical thyroid dysfunction is still a matter of debate, although it has been associated with various negative clinical out ... | 2010 | 20518189 |
| assessment of low-density lipoprotein oxidation, paraoxonase activity, and arterial distensibility in epileptic children who were treated with anti-epileptic drugs. | studies show that anti-epileptic drugs increase oxidative stress. thus, low-density lipoprotein oxidation increases and atherogenesis is induced. paraoxonase-associated high-density lipoprotein protects low-density lipoprotein and high-density lipoprotein oxidation. the effects of anti-epileptic drugs on paraoxonase activity has not been investigated yet. the aim of this study is to investigate the effect of anti-epileptic drugs on paraoxonase activity, lipid profiles, folat, vitamin b12, homocy ... | 2010 | 20519057 |
| a randomized simultaneous comparison of acceleromyography with a peripheral nerve stimulator for assessing reversal of rocuronium-induced neuromuscular blockade with sugammadex. | we investigated the relationship between acceleromyography and a peripheral nerve stimulator for measuring reversal in patients administered sugammadex following rocuronium. | 2010 | 20523215 |
| salvage surgery for local recurrence after chemoradiotherapy or radiotherapy in hypopharyngeal cancer patients. | this retrospective study aimed to assess the role of salvage surgery for local recurrence in hypopharyngeal cancer (hpc) patients who had received radiotherapy (rt) or concomitant chemoradiotherapy (crt) as an initial treatment. the local recurrence rate, salvage rate after local recurrence and overall survival rate were investigated in 104 hpc patients who received treatment between 1991 and 2005. local recurrence in the primary site was observed in 41 patients (rate, 39.4%) of whom only 12 cou ... | 2010 | 20495926 |
| a combined rt-pcr and dot-blot hybridization method reveals the coexistence of sjnnv and rgnnv betanodavirus genotypes in wild meagre (argyrosomus regius). | to detect the possible coexistence of striped jack nervous necrosis virus (sjnnv) and red-spotted grouper nervous necrosis virus (rgnnv) genotypes in a single fish, a methodology based on the combination of pcr amplification and blot hybridization has been developed and applied in this study. | 2010 | 20497277 |
| functional analysis of the highly antigenic outer capsid protein, hoc, a virus decoration protein from t4-like bacteriophages. | bacteriophage t4 is decorated with 155 copies of the highly antigenic outer capsid protein, hoc. the hoc molecule (40 kda) is present at the centre of each hexameric capsomer and provides a good platform for surface display of pathogen antigens. biochemical and modelling studies show that hoc consists of a string of four domains, three immunoglobulin (ig)-like and one non-ig domain at the c-terminus. biochemical data suggest that the hoc protein has two functional modules, a capsid binding modul ... | 2010 | 20497329 |
| better late than early: delayed translation of intron-encoded endonuclease i-tevi is required for efficient splicing of its host group i intron. | the td group i intron interrupting the thymidylate synthase (ts) gene of phage t4 is a mobile intron that encodes the homing endonuclease i-tevi. efficient rna splicing of the intron is required to restore function of the ts gene, while expression of i-tevi from within the intron is required to initiate intron mobility. three distinct layers of regulation temporally limit i-tevi expression to late in the t4 infective cycle, yet the biological rationale for stringent regulation has not been teste ... | 2010 | 20497330 |
| cord blood thyroid-stimulating hormone and free t4 levels in turkish neonates: is iodine deficiency still a continuing problem? | the objectives of this study were to determine the cord blood thyroid-stimulating hormone (tsh) and free t(4) (ft(4) ) levels in turkish neonates and to determine whether these variables reveal iodine deficiency. | 2010 | 20497362 |
| paclitaxel and carboplatin as neoadjuvant chemotherapy in patients with locally advanced breast cancer: a phase ii trial of the hellenic cooperative oncology group. | this phase ii study sought to evaluate the efficacy of the paclitaxel-carboplatin combination as neoadjuvant chemotherapy in patients with locally advanced breast cancer (labc). | 2010 | 20497922 |
| [bacteriophage t4: molecular aspects of bacterial cell infection and the role of capsid proteins]. | bacteriophage t4 of the myoviridae family is ubiquitous in the environment and living organisms. in microbiology it has become a universal research model for the mechanisms of many biological processes, including bacteriophage infection. t4 phage is a tailed phage, the most frequent bacteriophage group. it is made up of a head, a contractile tail, and dsdna. its tail is a complex structure composed of a collar and its whiskers, a tail tube, a base plate, short fibers, and tail fibers. all these ... | 2010 | 20498502 |
| preoperative local staging of esophageal carcinoma using dual-phase contrast-enhanced imaging with multi-detector row computed tomography: value of the arterial phase images. | to evaluate whether the accuracy of local extension (t-staging) of esophageal carcinoma could be improved with addition of arterial phase images by use of multi-detector row computed tomography. | 2010 | 20498545 |
| comprehensive effects of supplemented essential amino acids in patients with severe copd and sarcopenia. | aim of the study was to investigate whether or not oral supplementation of essential amino acids (eaas) may improve body composition, muscle metabolism, physical activity, cognitive function, and health status in a population of subjects with severe chronic obstructive pulmonary disease (copd) and sarcopenia. | 2010 | 20499791 |
| haemostasis in head and neck surgical procedures: valsalva manoeuvre versus trendelenburg tilt. | the aim of the study was to identify whether trendelenburg position helps detect any further bleeding points following valsalva manoeuvre in order to achieve adequate haemostasis in head and neck surgery. | 2010 | 20501015 |
| a randomized controlled trial of the effect of thyroxine replacement on cognitive function in community-living elderly subjects with subclinical hypothyroidism: the birmingham elderly thyroid study. | subclinical hypothyroidism (sch) and cognitive dysfunction are both common in the elderly and have been linked. it is important to determine whether t4 replacement therapy in sch confers cognitive benefit. | 2010 | 20501682 |
| intense therapy in patients with locally advanced esophageal cancer beyond hope for surgical cure: a prospective, multicenter phase ii trial of the swiss group for clinical cancer research (sakk 76/02). | there is no standard treatment for patients with locally advanced esophageal carcinoma without systemic metastasis in whom surgery is no longer considered a reasonable option. | 2010 | 20502056 |
| effect of sex, age, and anthropometric parameters on the size and shape of vertebrae in densitometric morphometry: results of the epolos study. | morphometric densitometry (morphometric x-ray absorptiometry - mxa) allows to identify vertebral fractures, based on the objective measurement of vertebral dimensions. | 2010 | 20502404 |
| [the "aktion t4" : in remembrance of victims of the healing and nursing institution in günzburg.] | objective: the aim of this article was to achieve a commemoration of patients of the healing and nursing institute in günzburg who were victims of "aktion t4". methods: on the basis of pre-defined criteria several individual patient documents were selected for this study and analyzed historically. most items of information concerning patient histories and diagnoses were obtained from the stock r 179 of the branch office of the federal archives in berlin lichterfelde. further supplementary inform ... | 2010 | 20502857 |
| comparison of testis structure, function and thyroid hormone levels in control c57bl/6 mice and anti-mullerian hormone over expressing mice. | anti-mullerian hormone (amh) is considered as a negative regulator of postnatal leydig cell (lc) differentiation, because amh over expressing mice (mt-hamh mice) testes are deficient in lc. therefore, in the present study mt-hamh mice was used as a model to examine the process of postnatal lc differentiation. testis structure-function studies were performed in age-matching mt-hamh and c57bl/6 (controls) mice; testicular components were quantified and circulating testosterone and thyroid hormone ... | 2010 | 20503178 |
| inflammatory breast cancer in italy: epidemiological and clinical aspects. | breast cancer represents the most common cancer among women in italy. in the last decade, an increase in incidence and a decrease in mortality from breast cancer have been observed in italy. these findings may be explained at least in part by the implementation of organized screening programs (smps). the screening programs are not diffused homogeneously throughout italy, where approximately 60% of the population in covered, which explains in part the different outcomes observed across italy. on ... | 2010 | 20503402 |
| quantification of uv-induced cyclobutane pyrimidine dimers using an oligonucleotide chip assay. | a lesion-specific enzyme-induced dna strand break assay was developed for an oligonucleotide chip for the determination of uvb-induced cyclobutane pyrimidine dimers (cpds). a 20-mer of fluorophore-labeled and biotinylated oligonucleotide was immobilized on the chip. cpds in dna on the chip were formed by uvb irradiation (312 nm). t4 endonuclease v (t4n5) was used to excise the cpd site as t4n5 sensitively and specifically detects cpds. the fluorophore-labeled dna fragments were detected by a las ... | 2010 | 20473657 |
| [feasibility to use of recombinant domains of immunoglobulin-like protein liga as a promising marker for serological testing of patients with leptospirosis]. | to clone the dna fragment encoding conservative domain of liga protein of leptospira interrogans into escherichia coli and to investigate antigenic properties of constructed chimeric protein. | 2010 | 20465007 |
| vaginal obliteration in a woman with a history of cutaneous t-cell lymphoma: the results of combined chemotherapy-induced gonadal toxicity and lymphoma relapse. | although ovarian failure commonly occurs following chemotherapy, the relation between menopause or related sexual activity in this failure is often overlooked. we report a case that emphasizes a clinically rare complication, the complete obliteration of the vagina. | 2010 | 20466296 |
| oncofetal fibronectin mrna is highly abundant in the blood of patients with papillary thyroid carcinoma and correlates with high-serum thyroid-stimulating hormone levels. | papillary thyroid carcinoma (ptc) cells express oncofetal fibronectin (onffn) mrna, which may be useful to detect circulating tumor cells. the objective of this study was to determine the fraction of ptc patients having onffn mrna in their peripheral blood and to determine if onffn mrna levels are correlated with the status of the disease or with thyroid-stimulating hormone (tsh) serum concentrations. | 2010 | 20470202 |
| management of gallbladder cancer. | resection is a means of improving survival in patients with gallbladder cancer. a more aggressive surgical approach, including resection of the gallbladder, liver, and regional lymph nodes, is advisable for patients with t1b to t4 tumors. aggressive resection is necessary because a patient's gallbladder cancer stage determines the outcome, not the surgery itself. therefore, major resections should be offered to appropriately selected patients. patients with advanced tumors or metastatic disease ... | 2010 | 20478489 |
| pattern and amount of change after orthodontic correction of upper front teeth 7 years postretention. | to investigate the amount and pattern of changes of maxillary front teeth 7 years postretention, which previously were retained with a bonded retainer. | 2010 | 20482345 |
| early headgear effect on the eruption pattern of maxillary second molars. | to test the hypothesis that the use of a combination headgear (hg) during the first phase of orthodontic treatment has no effect on the eruption pattern of the maxillary second permanent molars. | 2010 | 20482348 |
| age and the thyrotropin response to hypothyroxinemia. | some studies suggest altered pituitary functioning and tsh production with aging. | 2010 | 20484476 |
| sorafenib-induced hypothyroidism is associated with increased type 3 deiodination. | therapy with tyrosine kinase inhibitors is associated with thyroid dysfunction. decreased serum thyroid hormone levels during tyrosine kinase inhibitors are also observed in athyreotic patients with thyroid carcinoma. we therefore hypothesized that tyrosine kinase inhibitors may influence thyroid hormone metabolism. | 2010 | 20484486 |
| supplementation of farta sheep fed hay with graded levels of concentrate mix consisting of noug seed meal and rice bran. | the study was carried out at woreta, ethiopia, to determine feed intake, digestibility, body weight (bw) change, and profitability of farta sheep fed pasture hay alone or supplemented with graded levels of concentrate mix (cm) consisting of noug seed meal (nsm) and rice bran in 2:1 ratio. twenty yearling intact male farta sheep with bw of 16.9 +/- 1.68 kg (mean +/- sd) were used in randomized complete block design arranged into five blocks of four animals. the dietary treatments consisting of so ... | 2010 | 20490664 |
| identification of a new motif in family b dna polymerases by mutational analyses of the bacteriophage t4 dna polymerase. | structure-based protein sequence alignments of family b dna polymerases revealed a conserved motif that is formed from interacting residues between loops from the n-terminal and palm domains and between the n-terminal loop and a conserved proline residue. the importance of the motif for function of the bacteriophage t4 dna polymerase was revealed by suppressor analysis. t4 dna polymerases that form weak replicating complexes cannot replicate dna when the dgtp pool is reduced. the conditional let ... | 2010 | 20493878 |
| the folding cooperativity of a protein is controlled by its chain topology. | the three-dimensional structures of proteins often show a modular architecture comprised of discrete structural regions or domains. cooperative communication between these regions is important for catalysis, regulation and efficient folding; lack of coupling has been implicated in the formation of fibrils and other misfolding pathologies. how different structural regions of a protein communicate and contribute to a protein's overall energetics and folding, however, is still poorly understood. he ... | 2010 | 20495548 |
| paracrine signaling by glial cell-derived triiodothyronine activates neuronal gene expression in the rodent brain and human cells. | hypothyroidism in humans is characterized by severe neurological consequences that are often irreversible, highlighting the critical role of thyroid hormone (th) in the brain. despite this, not much is known about the signaling pathways that control th action in the brain. what is known is that the prohormone thyroxine (t4) is converted to the active hormone triiodothyronine (t3) by type 2 deiodinase (d2) and that this occurs in astrocytes, while th receptors and type 3 deiodinase (d3), which in ... | 2010 | 20458138 |
| [classification and characters of physical parameters of lifting-thrusting and twirling manipulations of acupuncture]. | by using "acupuncture manipulation information analyzing system", the traditional reinforcing and reducing manipulations of acupuncture described in classical chinese medical books were imitated and their parameters analyzed. it was found that in the oscillogram, the differences between the reinforcing and reducing manipulations (rapid-slow reinforcing-reducing skill, lifting-thrusting reinforcing-reducing skill) are the bigger duration of descending branch (t1) and smaller duration of ascending ... | 2010 | 20458910 |
| incorporation of gemcitabine and cytarabine into dna by dna polymerase beta and ligase iii/xrcc1. | 1-beta-d-arabinofuranosylcytosine (cytarabine, arac) and 2',2'-difluoro-2'-deoxycytidine (gemcitabine, dfdc), are effective cancer chemotherapeutic agents due to their ability to become incorporated into dna and then subsequently inhibit dna synthesis by replicative dna polymerases. however, the impact of these 3'-modified nucleotides on the activity of specialized dna polymerases has not been investigated. the role of polymerase beta and base excision repair may be of particular importance due ... | 2010 | 20459144 |
| partial pressure of carbon dioxide in the venous blood of young birds as a predictor of ascites susceptibility in broiler chickens. | in order to investigate the relationship between carbon dioxide tensions in the venous blood of young chickens and ascites susceptibility, one hundred day-old chickens from two pure broiler breeder lines differing in susceptibility to ascites syndrome were obtained and reared at low environmental temperature. weekly, blood samples were taken for the determination of blood gas parameters and plasma thyroid hormone levels. dead birds were examined for lesions of ascites daily and all survived bird ... | 2010 | 20460221 |
| detrusor activity is impaired during thoracic epidural analgesia after open renal surgery. | there are no data on lower urinary tract function during postoperative thoracic epidural analgesia (tea). because selected segmental blockade can be achieved with epidural analgesia, we hypothesized that lower urinary tract function remains unchanged during tea within segments t4-t11 after open renal surgery. | 2010 | 20461004 |
| cervical epidural injection complicated by syrinx formation: a case report. | case report and review of literature. | 2010 | 20461029 |
| xanthohumol, a prenylated chalcone from hops, modulates hepatic expression of genes involved in thyroid hormone distribution and metabolism. | in the present study, we analyzed the influence of xanthohumol (xn) on thyroid hormone (th) distribution and metabolism in rats. a potent and selective competition of xn for thyroxine (t4) binding to transthyretin (ic(50)=1 microm at 1.7 nm [(125)i]t4) was found in human and rat sera in vitro. female rats treated orally with xn showed increased hepatic expression of t4-binding globulin and decreased transthyretin and albumin. thyrotropin levels and hepatic type 1 deiodinase activity were moderat ... | 2010 | 20461738 |
| independent changes of thyroid hormones in blood plasma and cerebrospinal fluid after melatonin treatment in ewes. | the authors measured the effects of exogenous melatonin treatment on the concentrations of total (t) and free (f) fractions of thyroxine (t4) and triiodothyronine (t3) in cerebrospinal fluid (csf) and blood plasma as well as the expression of their binding/transporter protein, transthyretin (ttr), in the choroid plexus of ewes from may to august. melatonin implantation in may and july mainly prevented the decrease in plasma for ft3 and tt3 exhibited in untreated group, and induced a limited decr ... | 2010 | 20452012 |
| effects of magnesium on central arterial compliance in preeclampsia. | the objective of the study was to investigate the effect of mgso4 infusion on central arterial compliance, using radial artery applanation tonometry in women with preeclampsia. | 2010 | 20452485 |
| the effects of hypothyroidism on strength-duration properties of peripheral nerve. | axonal injury and/or demyelination commonly result in peripheral mononeuropathy, polyneuropathy or entrapment neuropathies in hypothyroidism. in this study, we aimed to measure the strength-duration time constants (sdtc) of motor and sensory fibres in patients with primary hypothyroidism before and after hormone replacement treatment. the motor and sensory sdtc and rheobase of 14 patients with overt hypothyroidism (tsh>10mg/ml, free t4<normal range, with positive thyroid autoantibodies) and 10 a ... | 2010 | 20452625 |
| impact of radiation dose on locoregional control and survival on squamous cell carcinoma of anal canal. | to perform a systematic analysis of clinical data of presentation, treatment, outcome, toxicity, survival and other associated prognostic factors of the patients of anal canal who received treatment at our hospital. | 2010 | 20452695 |
| in vivo assessment and potential diagnosis of xenobiotics that perturb the thyroid pathway: proteomic analysis of xenopus laevis brain tissue following exposure to model t4 inhibitors. | as part of a multi-endpoint systems approach to develop comprehensive methods for assessing endocrine stressors in vertebrates, differential protein profiling was used to investigate expression patterns in the brain of the amphibian model (xenopus laevis) following in vivo exposure to a suite of t4 synthesis inhibitors. we specifically address the application of two dimensional polyacrylamide gel electrophoresis (2d page), isobaric tags for relative and absolute quantitation (itraq) and lc-ms/ms ... | 2010 | 20452843 |
| t cell immunoglobulin- and mucin-domain-containing molecule-4 attenuates concanavalin a-induced hepatitis by regulating macrophage. | tim-4 is expressed primarily on apcs, including macrophages, and has been shown to play a critical role in t cell regulation. however, it remains unclear whether tim-4 also plays a role in the regulation of macrophage functions. in the present study, we investigated the effects of tim-4 on macrophage activity in con a-induced hepatitis in mice. we found that high levels of tim-4 expression were associated with a diminished serum level of alt in con a-induced hepatitis. in addition, adoptive tran ... | 2010 | 20453106 |
| elevated preoperative c-reactive protein predicts poor cancer specific survival in patients undergoing resection for non-small cell lung cancer. | although only the minority of patients with non-small cell lung cancer (nsclc) are suitable for surgical resection, it offers the best possibility of cure. the aim of this study was to examine the relationship between the clinicopathological status, the preoperative systemic inflammatory response, and survival in patients undergoing potentially curative resection for nsclc. | 2010 | 20453690 |
| coping with the unexpected at surgery. | in lung cancer surgery, it is best to avoid surprises; this requires knowledge of the reliability of preoperative assessment and careful planning. if this has been done, most of the more common situations should be manageable. if there is limited chest wall, mediastinal or n2 node involvement, one should proceed with resection. unanticipated t4 tumors or bulky pn2 disease should not come as a surprise, and such patients should be sent to a more experienced center. one has to be careful to practi ... | 2010 | 20387297 |
| [clinical studies on renal cell carcinoma]. | we reviewed 193 patients of renal cell carcinoma treated at osaka police hospital between 1990 and 2006. | 2010 | 20387516 |
| the center for alternatives to animal testing - europe (caat-eu): a transatlantic bridge for the paradigm shift in toxicology. | the center for alternatives to animal testing - europe (caat-eu) was founded based collaboration between the johns hopkins bloomberg school of public health and the university of konstanz. caat-eu, housed at the university of konstanz, will coordinate transatlantic activities to promote humane science in research and education, and participate, as partner or coordinator, in publicly and privately funded european projects. thomas hartung will serve as program liaison representing johns hopkins un ... | 2010 | 20390240 |
| isolation and characterization of differentially expressed transcripts from the suspension cells of oil palm (elaeis guineensis jacq.) in response to different concentration of auxins. | oil palm suspension cultures were initiated by transferring the gel-like friable embryogenic tissue onto liquid medium supplemented with auxins. in this study, transcripts that were differentially expressed in oil palm suspension cells cultured at different auxin concentrations were examined using suppression subtractive hybridization. total rna was first isolated from oil palm suspension cells proliferated in liquid medium with different hormone concentrations for 6 months. four different hormo ... | 2010 | 20390382 |
| laparoscopic rectal surgery: does immediate outcome differ in respect to sex? | this study analyzed the immediate postoperative outcome of the laparoscopic approach to the rectum in relation to the sex of the patient. | 2010 | 20305444 |
| accuracy of endoscopic ultrasound in preoperative staging of esophageal cancer: results from a referral center for early esophageal cancer. | endoscopic ultrasound (eus) has been regarded as the most accurate staging tool in esophageal cancer. staging results have a strong impact on the decision as to whether a patient should undergo endoscopic treatment, surgery alone, or neoadjuvant therapy. this retrospective study was conducted to analyze the accuracy of esophageal cancer staging using eus. | 2010 | 20306385 |
| effects of preoperative immunochemoradiotherapy and chemoradiotherapy on immune responses in patients with rectal adenocarcinoma. | we previously reported that preoperative chemoradiotherapy (crt) combined with intraoperative electron irradiation for ct3/t4 adenocarcinoma of the rectum reduced the local recurrence, with significant improvement of survival. radiotherapy has been reported to reduce immune function. we examined the effects of psk, a protein-bound polysaccharide, concomitant with preoperative crt on immune responses. | 2010 | 20393026 |
| biomechanical analysis of derotation of the thoracic spine using pedicle screws. | biomechanical analysis of derotational load-to-failure of pedicle screw (ps) instrumentation in cadaveric thoracic spinal segments. | 2010 | 20393385 |
| one-step, zero-background ligation-independent cloning intron-containing hairpin rna constructs for rnai in plants. | *the hairpin-based rna interference (rnai) technique plays an important role in exploring gene function in plants. although there are several methods for making hairpin rna (hprna) constructs, these methods usually need multiple relatively laborious, time-consuming or high-cost cloning steps. here we describe a one-step, zero-background ligation-independent cloning (oz-lic) method for making intron-containing hprna (ihprna) constructs by our vector prnai-lic. *to generate the ihprna constructs w ... | 2010 | 20406406 |
| primary antiphospholipid syndrome and thyroid involvement. | data on thyroid involvement in primary antiphospholipid syndrome are scarce and inconclusive. | 2010 | 20407389 |
| development of a high-throughput screening platform for dna 3'-phosphatases and their inhibitors based on a universal molecular beacon and quantitative real-time pcr. | dna 3'-phosphatases play a unique role in the repair of strand breaks induced by dna damaging agents, such as ionizing radiation or oxidative stress. in this paper, we present an efficient detection system for rapid screening of dna 3'-phosphatases and their inhibitors. a unique template substrate has been designed to hybridize with the universal molecular beacon (u-mb), and the detection process is carried out in a quantitative real-time pcr. the method is successfully applied to monitor the ac ... | 2010 | 20408165 |
| [disorders in thyroid hormone metabolism]. | the levels of the serum thyroid hormone (free t4 and free t3) are determined not only by thyroid hormone synthesis/secretion but also by their metabolism. thyroid hormone metabolism is mediated by three selenoproteins, selenodeiodinase type 1, 2, and 3 (d1, d2, and d3), the expression and function of which are tightly regulated in a tissue-specific manner. among them, d2 increases and d3 decreases the intracellular thyroid hormone levels, whereas d1 seems to play a role as a housekeeping/scaveng ... | 2010 | 20408442 |
| four different ways of alternative transcripts generation mechanism in adra1a gene. | the adra1a (alpha-1-adrenergic receptor) gene is one of the members of g protein-coupled receptor superfamily. alternative splicing of this gene was known to generate four transcript variants which code four isoforms with various c-terminal regions. in this study, we conducted expression analysis and evolutionary characterization of alternative transcripts of the adra1a gene. in total, 10 alternative transcripts were identified using experimental approaches and in silico analysis. among them, 6 ... | 2010 | 20410666 |
| the impact of speech therapy on asthma and allergic rhinitis control in mouth breathing children and adolescents. | to determine the impact of speech therapy on asthma and allergic rhinitis control in mouth breathing children and adolescents. | 2010 | 20449526 |
| prolactin inhibition at mid-lactation influences adiposity and thyroid function in adult rats. | maternal hypoprolactinemia at the end of lactation (a precocious weaning model) increases milk leptin transfer and results in overweight, leptin resistance, and secondary hypothyroidism at adulthood. we studied the effects of prolactin (prl) inhibition during mid-lactation (a partial malnutrition model) on milk leptin transfer, leptinemia, body composition, and thyroid function. lactating rats were treated with bromocryptine (bro, 1 mg/twice daily) or saline on days 7, 8, and 9 of lactation. off ... | 2010 | 20449792 |
| [progress in tripterygium wilfordiiand its bioactive components in the field of pharmacodynamics and pharmacology]. | in the trials of multi-glycoside of tripterygium wilfordii (gtw) in the field of pharmacodynamics, some clinical characteristics and symptoms, such as proteinuria, hematuria,joint pain, and skin damage, could be improved in the patients with various diseases including proliferative glomerulonephritis, lupus nephritis, rheumatoid arthritis, psoriasis and other immune-related diseases. in this review, it has been also reported to discuss the effects of gtw and triptolide (t4), which is a bioactive ... | 2010 | 20450057 |
| [atp-sensitive potassium channel function during the progression of pressure overload heart failure in rats]. | to investigate k(atp) channel function of cardiomyocytes isolated from the left ventricular wall of rats with or without abdominal aortic constriction at different time points under normal or simulated ischemic conditions. | 2010 | 20450563 |
| discrepancy between clinical staging through bimanual palpation and pathological staging after cystectomy. | objectives: in muscle invasive bladder cancer (mibc), careful clinical staging is essential for patient counseling and decision-making. bimanual palpation (bp) is an integral part and guideline advice of clinical staging. until now, however, the value of bp has never been studied. with this study, we aim to determine the accuracy of clinical staging through bp. methods: detailed clinical data were collected from a population-based series of 1,409 patients with mibc, diagnosed between 1989 and 20 ... | 2010 | 20451418 |
| peripheral blood dendritic cells and vascular endothelial growth factor in oral squamous cell carcinoma: correlation analysis and in vitro study. | vascular endothelial growth factor (vegf) may cause functional deficiency in dendritic cells (dcs) in vitro. the roles of peripheral blood dendritic cells (pbdcs) and vegf in patients with oral squamous cell carcinoma (oscc) are not well understood. the authors analysed the correlation between vegf and pbdc in 81 oscc patients. they assessed the effect of vegf on dc function in vitro. vegf levels were significantly increased in oscc patients compared with control subjects (p < 0.05), but pbdc le ... | 2010 | 20395112 |
| male breast cancer: a survey at the helsinki university central hospital during 1981-2006. | the purpose was to analyze the behavior of male breast cancer. | 2010 | 20397767 |
| the burden of comorbidity and the c-reactive protein levels in nonthyroidal illness syndrome with metabolic syndrome and atherosclerosis-related cardiovascular complications. | thyroid hormones undergo significant modifications during severe illnesses, and the low t3 levels are the hallmark of nonthyoidal illness syndrome (ntis), due to a reduced extrathyroidal conversion from t4. we examined 41 patients with ntis by a modified cumulative illness rating scale (cirs) and the measurement of ft3, ft4, tsh, and c-reactive protein (crp) levels. fifty-seven control subjects were enrolled. we observed reduced ft3 and increased ft4 levels in ntis patients (p < 0.05). the cirs ... | 2010 | 20398024 |
| comparison of uncalibrated arterial pressure waveform analysis with continuous thermodilution cardiac output measurements in patients undergoing elective off-pump coronary artery bypass surgery. | monitoring of cardiac output is required during anesthesia for off-pump coronary artery bypass (opcab) surgery. recently, flotrac, a new device for arterial pressure waveform analysis for cardiac output (apco) monitoring without external calibration, was developed. the authors have compared apco with stat-mode continuous cardiac output (scco) in patients undergoing opcab surgery. | 2010 | 20399114 |
| radioiodine plus recombinant human thyrotropin do not cause acute airway compression and are effective in reducing multinodular goiter. | recombinant human thyrotropin (rhtsh) reduces the activity of radioiodine required to treat multinodular goiter (mng), but acute airway compression can be a life-threatening complication. in this prospective, randomized, double-blind, placebo-controlled study, we assessed the efficacy and safety (including airway compression) of different doses of rhtsh associated with a fixed activity of 131i for treating mng. euthyroid patients with mng (69.3 +/- 62.0 ml, 20 females, 2 males, 64 +/- 7 years) r ... | 2010 | 20401438 |
| indirect reference intervals estimated from hospitalized population for thyrotropin and free thyroxine. | to establish indirect reference intervals from patient results obtained during routine laboratory work as an alternative to laborious and expensive producing of their own reference range values according to international instructions. | 2010 | 20401954 |
| the c-terminal domain of yeast high mobility group protein hmo1 mediates lateral protein accretion and in-phase dna bending. | the saccharomyces cerevisiae high mobility group protein hmo1 has two dna binding domains, box a and box b, and a lysine-rich c-terminal extension. among other functions, hmo1 has been implicated as a component of the rna polymerase i transcription machinery. we report here that hmo1 promotes dna apposition as evidenced by its stimulation of end-joining in the presence of t4 dna ligase. analysis of truncated hmo1 variants shows that enhanced dna end-joining requires the c-terminal domain but tha ... | 2010 | 20402481 |
| metabolic effects of second-generation antipsychotics in bipolar youth: comparison with other psychotic and nonpsychotic diagnoses. | despite known metabolic effects of second-generation antipsychotics (sgas) on children and adolescents, comparative effects in youth with different diagnoses remain underreported. we compared differences in metabolic changes three months after starting treatment with sgas in youth with bipolar disorder and with other psychotic and nonpsychotic disorders. | 2010 | 20402710 |
| [hand-assisted laparoscopic nephrectomy in challenging cases]. | to assess the outcome of hand-assisted laparoscopic nephrectomy in patients with significant complicating clinical factors. | 2010 | 20403284 |
| absent cd44v6 expression is an independent predictor of poor urothelial bladder cancer outcome. | cd44v6 is a cell surface protein involved in cell migration, cell adhesion, tumor progression and metastatic spread. we evaluated its role as a molecular marker for urothelial bladder cancer. | 2010 | 20403620 |
| ion-trap mass spectrometry for determination of 3,5,3'-triiodo-l-thyronine and 3,5,3',5'-tetraiodo-l-thyronine in neonatal rat cardiomyocytes. | our short report describes a method employing electrospray ion-trap mass spectrometry (ms) connected to a reversed phase (c(8)) hplc system for monitoring of 3,5,3'-triiodo-l-thyronine (t3) and its precursor 3,5,3',5'-tetraiodo-l-thyronine (t4) in neonatal rat cardiomyocytes. the experimental protocol allows simultaneous analysis of the free thyroid hormones in nanomolar concentration range and enables observation of their distribution in cultivation medium over time. the method is a useful tool ... | 2010 | 20403674 |
| cryoem structure of hsp104 and its mechanistic implication for protein disaggregation. | hsp104 is a ring-forming aaa+ machine that recognizes both aggregated proteins and prion-fibrils as substrates and, together with the hsp70 system, remodels substrates in an atp-dependent manner. whereas the ability to disaggregate proteins is dependent on the hsp104 m-domain, the location of the m-domain is controversial and its exact function remains unknown. here we present cryoem structures of two hsp104 variants in both crosslinked and noncrosslinked form, in addition to the structure of a ... | 2010 | 20404203 |
| use of the proposals of the international association for the study of lung cancer in the forthcoming edition of lung cancer staging system to predict long-term prognosis of operated patients. | to evaluate the utility of the proposals of the international association for the study of lung cancer (iaslc) in the forthcoming 7th edition of lung cancer staging system to classify patients submitted to radical surgical resection of non-small cell lung cancer and to compare their value in predicting long-term prognosis with the existing 6th edition of the american joint committee on cancer (ajcc)/union internationale contre le cancer (uicc) tnm classification. | 2010 | 20404615 |
| the role of adjuvant chemotherapy in patients with locally advanced (pt3, pt4a) and/or lymph node-positive bladder cancer. | to report the long-term follow up of patients with locally advanced bladder cancer treated with either adjuvant chemotherapy with gemcitabine/cisplatin (gc) or methotrexate, vinblastine, epirubicin, and cisplatin (mvec) or no additional treatment after radical cystectomy, to examine various survival endpoints and factors associated with long-term survival. | 2010 | 20405206 |
| prognostic factors in node-negative colorectal cancer: a retrospective study from a prospective database. | there is a need to identify a subgroup of high-risk patients with node-negative colorectal cancer who have a poor long-term prognosis and may benefit from adjuvant therapies. the aim of this study was to evaluate the prognostic impact of clinical and pathological parameters in a retrospective study from a prospective, continuous database of homogenously treated patients. | 2010 | 20405293 |
| superficial siderosis of the central nervous system: secondary progression despite successful surgical treatment, mimicking amyotrophic lateral sclerosis. case report and review. | superficial siderosis of the central nervous system is a rare disorder with hemosiderin deposition in the spinal and cranial leptomeninges and subpial layer, mostly from repetitive subarachnoid hemorrhage. progressive sensorineural deafness, cerebellar ataxia, and pyramidal signs comprise the typical clinical presentation. we describe a 47-year-old patient, who showed initial 2-year improvement after successful occlusion of an intradural bleeding source at t4. secondary progression of symptoms w ... | 2010 | 20414791 |
| [cloning of id4 gene expression regulation promoter and subcloning of recombinant id4 promoter luciferase reporter]. | the present study was aimed to clone id4 gene promoter and upstream regulatory region, and to construct a series of recombinant promoter-luciferase reporter for exploring the mechanism of id4 gene expression regulation. methods and results: the upstream 5' flanking sequence of 2242 bp from transcriptional start site (tss) and downstream 5' non-coding region of 212 bp on id4 gene were searched out and downloaded from human genome databank of ncbi using whole length of id4 gene cdna as a probe; on ... | 2010 | 20416180 |
| weight-of-evidence analysis of human exposures to dioxins and dioxin-like compounds and associations with thyroid hormone levels during early development. | thyroid hormones play a critical role in the proper development of brain function and cell growth. several epidemiological studies have been conducted to assess potential associations between pre- and post-natal exposure to dioxins or dioxin-like compounds (dlcs) and the levels of circulating thyroid hormones during early development. dioxins and dlcs include chlorinated dibenzo-p-dioxins, chlorinated dibenzofurans, and mono- and non-ortho polychlorinated biphenyls (pcbs). we identified a total ... | 2010 | 20416351 |
| transverse maxillary distraction in patients with periodontal pathology or insufficient tooth anchorage using custom-made devices. | we present our experience using modified hyrax devices for treating transverse deficiencies of the maxilla in adult patients with periodontal pathology or insufficient tooth anchorage. the surgical technique, clinical indications, and results are discussed. | 2010 | 20417007 |
| developmental regulation of gene expression in the thyroid gland of xenopus laevis tadpoles. | thyroid hormones (th) are the primary morphogen regulating amphibian metamorphosis. however, knowledge about molecular mechanisms regulating thyroid gland activity in anuran tadpoles is very scarce. in this study, we characterized gene expression profiles in thyroids of xenopus laevis tadpoles during spontaneous metamorphosis. using real-time pcr, elevated expression of slc5a5, tpo, tshr, and sar1a mrnas was detected at late prometamorphic and climax stages. for dio2 and dio3 but not dio1, devel ... | 2010 | 20417211 |
| ampullary tumours (ampullomas) in the elderly--an interdisciplinary problem. | ampullary cancer is one of the periampullary tumours with better prognosis, but relapses occur early in some patients. this study was carried out to assess whether pancreatoduodenectomy (pde, whipple operation) could be a safe therapeutic procedure for elderly patients with periampullary tumours. | 2010 | 20418556 |
| [excessive shivering after remifentanil infusion in a muscular karate player for tracheal resection and construction surgery]. | a 22-year-old muscular karate player was diagnosed to have a tracheal tumor with a diameter of 2.8 cm that existed 2 cm under the glottis and occupied 60% of his trachea. he was scheduled for trachea resection and construction surgery. after awake-fiber intubation, anesthesia was maintained by continuous infusion of propofol and remifentanil, together with thoracic epidural anesthesia (t4-5). after 431 minutes of surgery, 50 mm of trachea was rejected. to prevent him from extending his neck, the ... | 2010 | 20420146 |
| iscr regulates rnase ls activity by repressing rnla transcription. | the escherichia coli endoribonuclease ls was originally identified as a potential antagonist of bacteriophage t4. when the t4 dmd gene is defective, rnase ls cleaves t4 mrnas and antagonizes t4 reproduction. this rnase also plays an important role in rna metabolisms in e. coli. rnla is an essential gene for rnase ls activity, but the transcriptional regulation of this gene remains to be elucidated. an fe-s cluster protein, iscr, acts as a transcription factor and controls the expression of genes ... | 2010 | 20421606 |
| active and passive mechanisms of helicases. | in this work, we discuss the active or passive character of helicases. in the past years, several studies have used the theoretical framework proposed by betterton and julicher [betterton, m.d. and julicher, f. (2005) opening of nucleic-acid double strands by helicases: active versus passive opening. phys. rev. e, 71, 11904-11911.] to analyse the unwinding data and assess the mechanism of the helicase under study (active versus passive). however, this procedure has given rise to apparently contr ... | 2010 | 20423906 |
| link between pericardial effusion and attenuation of qrs voltage in patients with hypothyroidism. | to determine the clinical correlates of pericardial effusion (pe) and a low-voltage electrocardiogram (ecg) in patients with primary hypothyroidism. | 2010 | 20424451 |
| immediate post-mastectomy breast reconstruction followed by radiotherapy: risk factors for complications. | the objective is to prospectively determine the factors responsible for reconstruction failure and capsular contracture in mastectomized breast cancer patients who underwent immediate two-stage breast reconstruction with a tissue expander and implant, followed by radiotherapy. this is a multicenter, prospective, non-randomized study. between february 1998 and september 2006, we prospectively examined 141 consecutive patients, each of which received an implant after mastectomy, followed by chest ... | 2010 | 20424909 |
| simultaneous determination of l- and d-t4 using a sequential injection analysis/sensors system. | a sequential injection analysis system was selected for the simultaneous determination of l- and d-t4 in raw material and pharmaceutical products, because of the highest precision and accuracy, the lower consumption of sample and buffer, and the flexibility of selecting different buffers accordingly with the type of sensors utilized as detectors. an amperometric biosensor based on d-amino acid oxidase was used as detector for d-t4 and an immunosensor based on anti-l-t4 was used as detector for l ... | 2010 | 20426752 |
| carbocyclic potential dna minor groove binders and their biological evaluation. | the biological evaluation of carbocyclic minor groove binders 1-6 is described. the cytotoxicity of the obtained compounds was tested on mda-mb-231 breast cancer cells. the mechanism of action of compounds 1-6 was studied employing the topoisomerase i/ii inhibition assay and ethidium displacement assay using pbr322. determination of association constants was done using calf thymus dna, t4 coliphage dna, poly(da-dt)(2), and poly(dg-dc)(2). the effect of compounds 1-6 on the amidolytic activity of ... | 2010 | 20429779 |
| assessment of oral toxicity and safety of pentamethylchromanol (pmcol), a potential chemopreventative agent, in rats and dogs. | 2,2,5,7,8-pentamethyl-6-chromanol (pmcol) was administered by gavage in rats for 28 days at dose levels of 0, 100, 500, and 2000mg/kg/day. pmcol administration induced decreases in body weight gains and food consumption, hepatotoxicity (increased tbili, alb, alt, tp; increased relative liver weights; increased t4 and tsh), nephrotoxicity (increased bun and bun/creat, histopathology lesions), effect on lipid metabolism (increased chol), anemia, increase in wbc counts (total and differential), coa ... | 2010 | 20430063 |
| neonatal screening for congenital hypothyroidism: a retrospective hospital based study from bahrain. | neonatal screening programs for congenital hypothyroidism (ch) are now widespread in developed countries. | 2010 | 20432804 |
| late-onset circulatory dysfunction after thyroid hormone treatment in an extremely low birth weight infant. | late-onset circulatory dysfunction (lcd) is a phenomenon specific to premature infants and is characterized by sudden onset of hyponatremia, hypotension, oliguria and non-physiological weight gain, without an obvious cause, in premature infants after stabilization of circulation and respiration. the cause of lcd is not clear, but adrenal insufficiency in premature infants is a severe syndrome because steroid replacement therapy is often essential to treat the symptoms. we report a rare case of a ... | 2010 | 20432818 |