Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| study of the degradation activity and the strategies to promote the bioavailability of phenanthrene by sphingomonas paucimobilis strain 20006fa. | the present study describes the phenanthrene-degrading activity of sphingomonas paucimobilis 20006fa and its ability to promote the bioavailability of phenanthrene. s. paucimobilis 20006fa was isolated from a phenanthrene-contaminated soil microcosm. the strain was able to grow in liquid mineral medium saturated with phenanthrene as the sole carbon source, showing high phenanthrene elimination (52.9% of the supplied phenanthrene within 20 days). the accumulation of 1-hydroxy-2-naphthoic acid and ... | 2010 | 19609598 |
| gellan gum: a new biomaterial for cartilage tissue engineering applications. | gellan gum is a polysaccharide manufactured by microbial fermentation of the sphingomonas paucimobilis microorganism, being commonly used in the food and pharmaceutical industry. it can be dissolved in water, and when heated and mixed with mono or divalent cations, forms a gel upon lowering the temperature under mild conditions. in this work, gellan gum hydrogels were analyzed as cells supports in the context of cartilage regeneration. gellan gum hydrogel discs were characterized in terms of mec ... | 2010 | 19658177 |
| anticoagulating activities of low-molecular weight fuco-oligosaccharides prepared by enzymatic digestion of fucoidan from the sporophyll of korean undaria pinnatifida. | in spite of their potential as biologically active compounds, the high molecular mass and viscous natures of fucoidans have hampered their applications especially as a therapeutic agent. herein the fucoidan-degrading enzyme activities were partially purified from the cultured cells of sphingomonas paucimobilis pf-1 mainly by ammonium sulfate precipitation. this enzyme preparation degraded fucoidans from the korean undaria pinnatifida sporophyll into several low-molecular weight fuco-oligosacchar ... | 2010 | 20191353 |
| sphingomonas paucimobilis bacteremia in humans: 16 case reports and a literature review. | sphingomonas paucimobilis is a glucose-nonfermenting gram-negative bacillus that is widely distributed in both natural environment and hospitals. various infections in humans have been reported, but most have been limited to sporadic case reports. the aim of this study was to describe the clinical characteristics and manifestations of s. paucimobilis bacteremia. we also reviewed the literature on s. paucimobilis bacteremia. | 2010 | 20434121 |
| sphingomonas paucimobilis: a persistent gram-negative nosocomial infectious organism. | non-fermenting gram-negative bacilli create a significant problem in clinical settings, being the most widespread cause of nosocomial infections. they are opportunistic pathogens that take advantage of underlying conditions and diseases. sphingomonas paucimobilis, a non-fermenting gram-negative bacillus, is regarded as of minor clinical significance; however, many instances of infections with this organism can be found in the literature. infections include bacteraemia/septicaemia caused by conta ... | 2010 | 20434794 |
| inhibition of the plp-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation. | cycloserine (cs, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (plp)-dependent enzymes. two cs enantiomers are known; d-cycloserine (dcs, also known as seromycin) is a natural product that is used to treat resistant mycobacterium tuberculosis infections as well as neurological disorders since it is a potent nmda receptor agonist, and l-cycloserine (lcs) is a synthetic enantiomer whose usefulness as a drug has been hampered by ... | 2010 | 20445930 |
| detecting variants with metabolic design, a new software tool to design probes for explorative functional dna microarray development. | microorganisms display vast diversity, and each one has its own set of genes, cell components and metabolic reactions. to assess their huge unexploited metabolic potential in different ecosystems, we need high throughput tools, such as functional microarrays, that allow the simultaneous analysis of thousands of genes. however, most classical functional microarrays use specific probes that monitor only known sequences, and so fail to cover the full microbial gene diversity present in complex envi ... | 2010 | 20860850 |
| comparison of maldi tof with conventional identification of clinically relevant bacteria. | a completely new approach to diagnose microbial agents at least one day earlier based on mass spectrometric analysis becomes possible in the microbiology laboratory: maldi tof: matrix-assisted laser desorption/ionisation time-of-flight. comparison between results of the new procedure with those obtained by conventional testing is mandatory. | 2010 | 20924806 |
| use of reporter-gene based bacteria to quantify phenanthrene biodegradation and toxicity in soil. | a phenanthrene-degrading bacterium, sphingomonas paucimobilis epa505 was used to construct two fluorescence-based reporter strains. strain d harboring gfp gene was constructed to generate green fluorescence when the strain started to biodegrade phenanthrene. strain s possessing gef gene was designed to die once phenanthrene biodegradation was initiated and thus to lose green fluorescence when visualized by a live/dead cell staining. confocal laser scanning microscopic observation followed by ima ... | 2010 | 21093134 |
| application of the mixture design to optimise the formulation of active consortia to decolorize azo-dye methyl red. | with the aid of analysis software (minitab 14.0), the formulation of pure culture in mineral salts medium (msm) can be optimized for several responses and the best formulation can be obtained. the influence of the different mixtures of three strains in the pure culture in msm on the flavor components in decolorization of methyl red (with initial total cell density fixed at od600 = 1 and in addition of 750 ppm of dye) was studied using equilateral triangle diagram and mixture experimental design ... | 2010 | 21123913 |
| usefulness of platelet (plt) pathogen inactivation technology: a case of sphingomonas paucimobilis inactivation in an apheresis plt unit. | 2010 | 21182632 | |
| bromhidrosis induced by sphingomonas paucimobilis: a case report. | bromhidrosis is a clinical disorder characterized by excessive or abnormal foul axillary odour due to the interaction of apocrine glands with micro-organisms which causes a serious personal and social handicap for affected people. we present the case of a 50-year-old caucasian female with bromhidrosis. the patient referred that this symptom had begun two months previously. her past treatments included antibacterial soap, topical antibacterial agents and perfumes, but none of these relieved the p ... | 2009 | 19822101 |
| [effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium]. | the effect of the inoculant strain sphingomonas paucimobilis 20006fa on the bacterial composition of a phenanthrene-degrading consortium obtained from a pristine soil in sequencing batch cultures was studied. inoculated (f200+1) and non-inoculated (f200) phenanthrene-degrading consortia, were obtained. bacterial diversity of consortia was studied at cultivable (phenotype and genotype characterization) and non-cultivable (pcr-dgge) levels. during the successive cultures, a loss in the phenanthren ... | 2009 | 19623894 |
| microbial conversion of glucose to a novel chemical building block, 2-pyrone-4,6-dicarboxylic acid. | 2-pyrone-4,6-dicarboxylic acid (pdc) is a catabolic intermediate in sphingobium sp. syk-6 (previously characterized as sphingomonas paucimobilis syk-6), which is a degrader of lignin-derived aromatic compounds. recently, pdc has been also characterized as a novel starting material for several potentially useful synthetic polymers. in a previous study, we constructed a biosynthetic system in which pdc was generated efficiently from a chemically synthesized compound, protocatechuate. in order to d ... | 2009 | 19281857 |
| the external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and hsan1 mutant mimics. | sphingolipid biosynthesis begins with the condensation of l-serine and palmitoyl-coa catalyzed by the plp-dependent enzyme serine palmitoyltransferase (spt). mutations in human spt cause hereditary sensory autonomic neuropathy type 1, a disease characterized by loss of feeling in extremities and severe pain. the human enzyme is a membrane-bound hetereodimer, and the most common mutations are located in the enzymatically incompetent monomer, suggesting a "dominant" or regulatory effect. the molec ... | 2009 | 19376777 |
| distribution and biodegradation of polycyclic aromatic hydrocarbons in contaminated sites of hisar (india). | fifty-two soil samples were collected from various location of the hisar city. these samples were analysed for six polycyclic aromatic hydrocarbons (naphthalene, acenaphthene, phenanthrene, anthracene, fluoranthene, and pyrene). total mean concentration of six pahs varied from 51.79 to 148.82 mg kg(-1) dry weight of the soil, pah concentration was higher in soil samples from local auto market while lower concentration was recorded in agricultural soil. effect ofph (5.0 to 9.0), temperature (20 d ... | 2009 | 19405388 |
| sphingomonas paucimobilis bacteraemia and septic arthritis in a diabetic patient presenting with septic pulmonary emboli. | sphingomonas paucimobilis, a yellow-pigmented, aerobic, glucose non-fermenting, gram-negative bacillus, is a rare cause of human infection normally associated with immunocompromised hosts. we report a case of bacteraemia and septic arthritis in a 47-year-old diabetic man who presented with septic pulmonary emboli due to s. paucimobilis. the patient had an initial presentation of fever, right knee pain, coughing, dyspnoea and chest pain. the infection was treated successfully by surgical debridem ... | 2009 | 19528166 |
| evaluation of antibacterial activity, phenol and flavonoid contents of thespesia populnea flower extracts. | flavonoids which were reported as having many pharmacological activities, antimicrobial, antioxidant, cytotoxic, chemoprevention activities and they possess strong antiproliferative effects related to inhibition of cell cycle progression and apoptosis induction. on the basis of this thespesia populnea (l.) sol. ex correa (family-malvaceae) was selected and it is having the major composition of flavonoids and the antibacterial activity of methanolic extract of thespesia populnea flowers was inves ... | 2009 | 19553175 |
| evaluation of the airborne bacterial population in the periodically confined antarctic base concordia. | the environmental airborne bacterial population in relation to human confinement was investigated over a period of 1 year in the concordia research station, which is located on the eastern antarctic plateau. the unique location of the station makes it suitable for different research domains such as glaciology, atmospheric sciences, astronomy, etc. furthermore, it is used as a test bed for long-duration spaceflights to study the physiologic and psychological adaptation to isolated environments. a ... | 2009 | 18972149 |
| sphingomonas paucimobilis bloodstream infections associated with contaminated intravenous fentanyl. | nationally distributed medications from compounding pharmacies, which typically adhere to less stringent quality-control standards than pharmaceutical manufacturers, can lead to multistate outbreaks. we investigated a cluster of 6 patients in a maryland hospital who had sphingomonas paucimobilis bloodstream infections in november 2007. of the 6 case-patients, 5 (83%) had received intravenous fentanyl within 48 hours before bacteremia developed. cultures of unopened samples of fentanyl grew s. pa ... | 2009 | 19116043 |
| water-borne sphingomonas paucimobilis epidemic in an intensive care unit. | 2009 | 19232740 | |
| intestinal obstruction associated with chronic peritonitis caused by sphingomonas paucimobilis. | we describe a very rare case of chronic peritonitis with secondary adhesive intestinal obstruction caused by sphingomonas paucimobilis in a healthy 28-year-old chinese man. this bacillus has not been described as a cause of spontaneous peritonitis in healthy people. it was an asymptomatic, generalized, and slow-growing peritonitis causing peritoneal adherens and at the end intestinal occlusion that needed surgical adhesiolysis. | 2009 | 26192291 |
| arbuscular mycorrhiza and petroleum-degrading microorganisms enhance phytoremediation of petroleum-contaminated soil. | while plants can phytoremediate soils that are contaminated with petroleum hydrocarbons, adding microbes to remediate contaminated sites with petroleum-degrading microorganisms and arbuscular mycorrhizal fungi (amf) is not well understood. the phytoremediation of arabian medium crude oil (aco) was done with a lolium multiflorum system inoculated with an amf (glomus intraradices) and a mixture of petroleum-degrading microorganisms--the bacterium, sphingomonas paucimobilis (sp) and the filamentous ... | 2008 | 19260211 |
| [a rare cause of nosocomial bacteremia: sphingomonas paucimobilis]. | sphingomonas paucimobilis, is a yellow-pigmented, aerobic, non-fermentative, non-spore-forming, gram-negative bacillus. infections by s. paucimobilis which is widely found in nature and hospital environments are rarely serious or life threatening. in this report we present a case of hospital acquired bloodstream infection due to s. paucimobilis. the patient had a history of hydrocephalus diagnosed at sixth months of his birth and had experienced two ventriculoperitoneal shunt surgery. he was hos ... | 2008 | 19149092 |
| assessment of inhibitory effects of fluoride-coated tubes on biofilm formation by using the in vitro dental unit waterline biofilm model. | this study aimed to establish an in vitro model to simulate biofilms formed in dental unit waterlines (duwls) and to investigate the ability of polyvinylidene fluoride (pvdf)-coated tubes to inhibit biofilm formation using this model. the water and biofilm samples were obtained from duwls which had been clinically used for 2.5 years, and the predominant bacteria were identified. a conventional polyurethane tube was incubated for 24 to 96 h in the mixed flora of isolated bacteria, and the optimal ... | 2008 | 18676694 |
| sphingomonas paucimobilis peritonitis: a case report and literature review. | 2008 | 18708553 | |
| sphingomonas paucimobilis associated with localised calf myositis. | 2008 | 18796597 | |
| clinical features and treatment outcomes of infections caused by sphingomonas paucimobilis. | 2008 | 18808348 | |
| acclimated biomass that degrades sulfonated naphthalene formaldehyde condensate. | a number of aerobic species were isolated from textile industry activated sludge wastewater. the bacterial consortium was acclimated during seven days before testing its capacity of sulfonated naphthalene-formaldehyde condensate (snfc) recalcitrant compound degradation. snfc's degradation was evaluated by using different techniques including: vapour pressure osmometry, spectroscopy uv-visible and chemical oxygen demand (cod). the degradation of snfc by acclimated bacterial consortium was determi ... | 2008 | 18819646 |
| biodegradation kinetics of select polycyclic aromatic hydrocarbon (pah) mixtures by sphingomonas paucimobilis epa505. | many contaminated sites commonly have complex mixtures of polycyclic aromatic hydrocarbons (pahs) whose individual microbial biodegradation may be altered in mixtures. biodegradation kinetics for fluorene, naphthalene, 1,5-dimethylnaphthalene and 1-methylfluorene were evaluated in sole substrate, binary and ternary systems using sphingomonas paucimobilis epa505. the first order rate constants for fluorene, naphthalene, 1,5-dimethylnaphthalene, and 1-methylfluorene were comparable; yet monod para ... | 2008 | 17534722 |
| septic shock caused by sphingomonas paucimobilis bacteremia in a patient with hematopoietic stem cell transplantation. | sphingomonas paucimobilis is an aerobic gram-negative bacillus that causes a variety of infections in healthy as well as in immunocompromised individuals. the organism is usually susceptible to tetracycline, chloramphenicol, aminoglycosides, trimethoprim-sulfamethoxazole, and carbapenems. however, resistance to penicillins and the first-generation cephalosporins is commonly encountered. reported here is a patient with acute myeloid leukemia who developed s. paucimobilis bacteremia complicated by ... | 2008 | 17605729 |
| interaction of higher plant (jute), electrofused bacteria and mycorrhiza on anthracene biodegradation. | the interaction of bacteria, mycorrhiza and jute (corchotus capsulari, a higher plant) to reduce anthracene in different concentrations of spiked soils was investigated. dominant indigenous bacterium (pseudomonas sp.) isolated in the rhizosphere of jute was electrofused with anthracene degraders (sphingomonas paucimobilis and pseudomonas aeruginosa) which were able to produce different types of biosurfactants. the highest population (56 x 10(5)cfu/g) was found in the planted soil with the inocul ... | 2008 | 17662599 |
| effects of the inoculant strain sphingomonas paucimobilis 20006fa on soil bacterial community and biodegradation in phenanthrene-contaminated soil. | the effects of the inoculant strain sphingomonas paucimobilis 20006fa (isolated from a phenanthrene-contaminated soil) on the dynamics and structure of microbial communities and phenanthrene elimination rate were studied in soil microcosms artificially contaminated with phenanthrene. the inoculant managed to be established from the first inoculation as it was evidenced by denaturing gradient gel electrophoresis analysis, increasing the number of cultivable heterotrophic and pah-degrading cells a ... | 2008 | 17694405 |
| second step of hydrolytic dehalogenation in haloalkane dehalogenase investigated by qm/mm methods. | mechanistic studies on the hydrolytic dehalogenation catalyzed by haloalkane dehalogenases are of importance for environmental and industrial applications. here, car-parrinello (cp) and oniom hybrid quantum-mechanical/molecular mechanics (qm/mm) are used investigate the second reaction step of the catalytic cycle, which comprises a general base-catalyzed hydrolysis of an ester intermediate (ei) to alcohol and free enzyme. we focus on the enzyme linb from sphingomonas paucimobilis ut26, for which ... | 2008 | 17729274 |
| the negative interaction between the degradation of phenanthrene and tricyclazole in medium, soil and soil/compost mixture. | to assess the co-catabolism of phenanthrene and tricyclazole in different samples, the interaction during the degradation of phenanthrene and tricyclazole were investigated in medium, soil and soil/spent mushroom compost (smc) mixture. generally, tricyclazole showed a negative influence on the activity of phenanthrene dioxygenase and it inhibited the degradation of phenanthrene prominently, both in cultures of phenanthrene catabolic isolates (sphingomonas paucimobilis zx4 and the mixed flora m1) ... | 2008 | 18189158 |
| extracellular polysaccharides produced by cooling water tower biofilm bacteria and their possible degradation. | the extracellular polymers (eps) of biofilm bacteria that can cause heat and mass transfer problems in cooling water towers in the petrochemical industry were investigated. in addition, these microorganisms were screened for their ability to grow and degrade their own eps and the eps of other species. twelve bacteria producing the most eps were isolated from cooling water towers and characterized biochemically by classic and commercial systems. these were species of pseudomonas, burkholderia, ae ... | 2008 | 18256966 |
| a case of postoperative sphingomonas paucimobilis endophthalmitis after cataract extraction. | to report a case of an acute onset of delayed postoperative endophthalmitis that was caused by sphingomonas paucimobilis. | 2008 | 18323709 |
| quantitative structure-activity relationships for kinetic parameters of polycyclic aromatic hydrocarbon biotransformation. | quantitative structure-activity relationships (qsars) were developed for three monod-type parameters--qmax, ks, and qmax/ks--that express the kinetics of polycyclic aromatic hydrocarbon (pah) biotransformation by sphingomonas paucimobilis strain epa505. the training sets contained high-quality experimental values of the kinetic parameters for 20 unsubstituted and methylated pahs as well as values of 41 meaningful molecular descriptors. a genetic function approximation algorithm was used to devel ... | 2008 | 18366261 |
| optimization of gellan gum production by sphingomonas paucimobilis atcc 31461 with nonionic surfactants using central composite design. | the effect of nonionic surfactants on gellan production by sphingomonas paucimobilis was studied by the addition of 0.5, 0.75, 1.0, 1.25 and 1.5 g/l surfactants to shake flask culture. the nonionic surfactants tween 80, tween 40 and triton x-100 improved gellan production by s. paucimobilis, and the maximum yield (10.44 g/l) was obtained with triton x-100 at 0.75 g/l compared with that of the control fermentation (8.63 g/l) without surfactant. the do profiles associated with gellan production in ... | 2008 | 18397769 |
| isolation and characterization of marine bacterial strain degrading fucoidan from korean undaria pinnatifida sporophylls. | in spite of an increasing interest in fucoidans as biologically active compounds, no convenient commercial sources with fucoidanase activity are yet available. a marine bacterial strain that showed confluent growth on a minimal medium containing fucoidan, prepared from korean undaria pinnatifida sporophylls, as the sole carbon source was isolated and identified based on a 16s rdna sequence analysis as a strain of sphingomonas paucimobilis, and named sphingomonas paucimobilis pf-1. the strain dep ... | 2008 | 18467852 |
| characterization of ligv essential for catabolism of vanillin by sphingomonas paucimobilis syk-6. | the vanillin dehydrogenase gene (ligv), which conferred the ability to transform vanillin into vanillate on escherichia coli, was isolated from sphingomonas paucimobilis syk-6. the ligv gene consists of a 1,440-bp open reading frame encoding a polypeptide with a molecular mass of 50,301 da. the deduced amino acid sequence of ligv showed about 50% identity with the known vanillin dehydrogenases of pseudomonas vanillin degraders. the gene product of ligv (ligv) produced in e. coli preferred nad+ t ... | 2007 | 17928721 |
| nosocomial outbreak of sphingomonas paucimobilis bacteremia in a hemato/oncology unit. | nosocomial sphingomonas paucimobilis infections can arise from contaminated water and the contaminated hands of hospital staff. within a 1-month period, we isolated six s. paucimobilis strains, including four from blood cultures of four patients and two from hospital environment specimens including tap water and a bathtub in a hemato/oncology unit. we described here these strains' molecular epidemiological analyses by pulsed-field gel electrophoresis (pfge) and antibiotic susceptibilities by e-t ... | 2007 | 18032843 |
| aerobic degradation of lindane (gamma-hexachlorocyclohexane) in bacteria and its biochemical and molecular basis. | gamma-hexachlorocyclohexane (gamma-hch, also called gamma-bhc and lindane) is a halogenated organic insecticide that causes serious environmental problems. the aerobic degradation pathway of gamma-hch was extensively revealed in bacterial strain sphingobium japonicum (formerly sphingomonas paucimobilis) ut26. gamma-hch is transformed to 2,5-dichlorohydroquinone through sequential reactions catalyzed by lina, linb, and linc, and then 2,5-dichlorohydroquinone is further metabolized by lind, line, ... | 2007 | 17634937 |
| degradation of 3-o-methylgallate in sphingomonas paucimobilis syk-6 by pathways involving protocatechuate 4,5-dioxygenase. | sphingomonas paucimobilis syk-6 converts vanillate and syringate to protocatechuate and 3-o-methylgallate (3mga), respectively. 3mga is metabolized via multiple pathways involving 3mga 3,4-dioxygenase, protocatechuate 4,5-dioxygenase (ligab), and gallate dioxygenase whereas protocatechuate is degraded via the protocatechuate 4,5-cleavage pathway. here the secondary role of ligab in syringate metabolism is investigated. the reaction product of 3mga catalyzed by his-tagged ligab was identified as ... | 2007 | 17645527 |
| bacterial contamination of water in dental unit reservoirs. | the aim of this study was bacteriological assessment of water in dental unit reservoirs--concentration and composition of the aerobe and facultative anaerobe bacterial microflora. reservoir water samples were taken from 25 units. bacterial flora were determined with the plate culture method. bacteria were identified with biochemical microtests: api 20e, api 20ne (biomérieux, france) and gp2 microplatetm (biolog, usa). the concentration of total bacteria isolated from one site was 201,039 cfu/ml, ... | 2007 | 17655191 |
| 4-sulfomuconolactone hydrolases from hydrogenophaga intermedia s1 and agrobacterium radiobacter s2. | the 4-carboxymethylen-4-sulfo-but-2-en-olide (4-sulfomuconolactone) hydrolases from hydrogenophaga intermedia strain s1 and agrobacterium radiobacter strain s2 are part of a modified protocatechuate pathway responsible for the degradation of 4-sulfocatechol. in both strains, the hydrolase-encoding genes occur downstream of those encoding the enzymes that catalyze the lactonization of 3-sulfomuconate. the deduced amino acid sequences of the 4-sulfomuconolactone hydrolases demonstrated the highest ... | 2007 | 17660282 |
| molecular characterization of membrane-associated soluble serine palmitoyltransferases from sphingobacterium multivorum and bdellovibrio stolpii. | serine palmitoyltransferase (spt) is a key enzyme in sphingolipid biosynthesis and catalyzes the decarboxylative condensation of l-serine and palmitoyl coenzyme a (coa) to form 3-ketodihydrosphingosine (kds). eukaryotic spts comprise tightly membrane-associated heterodimers belonging to the pyridoxal 5'-phosphate (plp)-dependent alpha-oxamine synthase family. sphingomonas paucimobilis, a sphingolipid-containing bacterium, contains an abundant water-soluble homodimeric spt of the same family (h. ... | 2007 | 17557831 |
| the structure of serine palmitoyltransferase; gateway to sphingolipid biosynthesis. | sphingolipid biosynthesis commences with the condensation of l-serine and palmitoyl-coa to produce 3-ketodihydrosphingosine (kds). this reaction is catalysed by the plp-dependent enzyme serine palmitoyltransferase (spt; ec 2.3.1.50), which is a membrane-bound heterodimer (spt1/spt2) in eukaryotes such as humans and yeast and a cytoplasmic homodimer in the gram-negative bacterium sphingomonas paucimobilis. unusually, the outer membrane of s. paucimobilis contains glycosphingolipid (gsl) instead o ... | 2007 | 17559874 |
| a novel pathway for the biodegradation of gamma-hexachlorocyclohexane by a xanthomonas sp. strain ich12. | to isolate gamma-hexachlorocyclohexane (hch)-degrading bacteria from contaminated soil and characterize the metabolites formed and the genes involved in the degradation pathway. | 2007 | 17578411 |
| [contamination with sphingomonas paucimobilis: about seven cases isolated in conservation and transport mediums of corneal grafts]. | from september to december 2004, contaminations were found in fifteen conservation and transport mediums of corneal grafts at the tissue bank of brest, including seven by sphingomonas paucimobilis. the pulsed-field gel electrophoresis made it possible to establish the genotypic profiles of each strain and to compare them. similarities were found between certain strains of the contaminated mediums and those of the thermostated double boiler of the tissue bank. the link between the contamination a ... | 2007 | 16631318 |
| optimization of nutrients for gellan gum production by sphingomonas paucimobilis atcc-31461 in molasses based medium using response surface methodology. | a molasses based medium for the production of gellan by sphingomonas paucimobilis atcc-31461 was developed. placket-burman design criterion was applied to study the effect of various nutrient supplements on gellan production using molasses. among the 20 variables tested, molasses, tryptone, casaminoacid, disodium hydrogen orthophosphate and manganese chloride showed significant effect on gellan production. a central composite design was applied to determine the optimum concentrations of the sign ... | 2007 | 16707262 |
| enhanced degradation of a mixture of polycyclic aromatic hydrocarbons by a defined microbial consortium in a two-phase partitioning bioreactor. | biological treatment methods are effective at destroying polycyclic aromatic hydrocarbons (pahs), and some of the highest rates of pah degradation have been achieved using two-phase-partitioning bioreactors (tppbs). tppbs consist of a cell-containing aqueous phase and a biocompatible and immiscible organic phase that partitions toxic and/or recalcitrant substrates to the cells based on their metabolic demand and on maintaining the thermodynamic equilibrium of the system. in this study, the degra ... | 2007 | 16758271 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| deproteinization of gellan gum produced by sphingomonas paucimobilis atcc 31461. | deproteinization is a technical bottleneck in the purification of viscous water-soluble polysaccharides. the aim of this work is to provide an appropriate approach to deproteinize crude gellan gum. several methods of deproteinization were investigated, including sevag method, alkaline protease, papain and neutral protease. the results revealed that sevag method had high deproteinization efficiency (87.9%), but it showed dissatisfactory recovery efficiency of gellan gum (28.6%), which made it les ... | 2007 | 17069918 |
| measurement of biodegradability parameters for single unsubstituted and methylated polycyclic aromatic hydrocarbons in liquid bacterial suspensions. | substrate depletion experiments were conducted to characterize aerobic biodegradation of 20 single polycyclic aromatic hydrocarbons (pahs) by induced sphingomonas paucimobilis strain epa505 in liquid suspensions. pahs consisted of low molecular weight, unsubstituted, and methyl-substituted homologs. a material balance equation containing the andrews kinetic model, an extension of the monod model accounting for substrate inhibition, was numerically fitted to batch depletion data to estimate extan ... | 2007 | 17115446 |
| kinetics of biodegradation of binary and ternary mixtures of pahs. | the kinetics of biodegradation of mixtures of polycyclic aromatic hydrocarbons (pahs) by sphingomonas paucimobilis strain epa505 were investigated. the investigation focused on three- and four-ring pahs, specifically 2-methylphenanthrene, fluoranthene, and pyrene. uptake rates in aerobic batch suspended cultivations were measured for the individual pahs and their binary and ternary mixtures. it was observed that kinetics were influenced by the mixture composition and the kinetic properties of th ... | 2007 | 17115447 |
| genetic and biochemical investigations on bacterial catabolic pathways for lignin-derived aromatic compounds. | lignins are the most abundant aromatic compounds in nature, and their decomposition is essential to the terrestrial carbon cycle. white rot fungi secreting phenol oxidases are assumed to be involved in the initial degradation of native lignin, whereas bacteria play a main role in the mineralization of lignin-derived low-molecular-weight compounds in soil. there are a number of reports on the degradation pathways for lignin-derived aromatic compounds, but their catabolism has not been enzymatical ... | 2007 | 17213657 |
| biological pretreatment with two bacterial strains for enzymatic hydrolysis of office paper. | the cellulose-hydrolyzing strains, sphingomonas paucimobilis mk1 and bacillus circulans mk2, were separated from soil and were grown together in a single culture plate. growth b. circulans mk2 in liquid culture required symbiosis with s. paucimobilis mk1. biological pretreatment with the combined strain suspension after the liquid culture improved enzymatic hydrolysis of office paper from municipal wastes. sugar recovery by s. paucimobilis mk1 (51%) was 1.4 times higher than that of the untreate ... | 2007 | 17487532 |
| periplasmically located α-santonin binding factor in sphingomonas paucimobilis strain s atcc 43388. | a marked reduction in uptake of α-santonin, accompanied by loss of ability of cells to transform the substrate, is observed on shocking sphingomonas paucimobilis strain s atcc 43388 cells by freeze - thaw method. the shock fluid shows a 26% quench in fluorescence at 350nm on incubation with the substrate. addition of shock fluid to the freeze thawed cells restores both uptake as well as transformation of α-santonin to near normal. | 2007 | 23100690 |
| bacterial decontamination of duwl biofilm using oxygenal 6. | the aim of this study was bacteriological assessment of the dental unit waterlines (duwl) biofilm - concentration and composition of the aerobe and facultative anaerobe bacterial microflora, and evaluation of the influence of a disinfecting product, oxygenal 6, on the biofilm composition. tubing fragments were taken from 25 units twice, before and after disinfection, and bacterial suspension of the biofilm was obtained from the samples. the bacterial flora was determined with the plate culture m ... | 2006 | 16841887 |
| postoperative endophthalmitis caused by sphingomonas paucimobilis. | we present a case in which a new organism, sphingomonas paucimobilis, caused endophthalmitis after phacoemulsification in a 73-year-old woman. the case shows a recurrent acute endophthalmitis with complete resolution only after vitrectomy. this organism has not been described as a cause of endophthalmitis and was resistant to initial medical management. we also describe an interaction between this organism and a co-infective organism that may account for the unusual clinical course. | 2006 | 16857516 |
| effects of a small electric current on sterilization of a dental unit water line. | in the present study, water was circulated in a simulated dental unit water line with electrifying a small current. the morphology of the biofilm developed on inner surface of the water line and the number of heterotrophic bacteria were investigated to elucidate the effect of a low level electric current on the biofilms formation associated with bacteria reproduction. destruction and malconformation of biofilms by electrification was observed using sem, in addition to deformation and hypertrophy ... | 2006 | 16913572 |
| statistical approach to optimization of fermentative production of gellan gum from sphingomonas paucimobilis atcc 31461. | gellan gum, a high-molecular-weight anionic linear polysaccharide produced by pure-culture fermentation from sphingomonas paucimobilis atcc 31461, has elicited industrial interest in recent years as a high-viscosity biogum, a suspending agent, a gelling agent, and an agar substitute in microbial media. in this paper we report on the optimization of gellan gum production using a statistical approach. in the first step, the one factor-at-a-time method was used to investigate the effect of medium c ... | 2006 | 17046526 |
| gram-negative bacteria from patients seeking medical advice in stockholm after the tsunami catastrophe. | microbiological cultures from 229 patients seeking medical advice in stockholm after the tsunami catastrophe of december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative bacilli were the most common findings from wound cultures. common human pathogens such as escherichia coli, proteus species, klebsiella spp., and pseudomonas aeruginosa were isolated. more rare species of gram-negative bacilli, e.g. myroides odoratus, sp ... | 2006 | 16798691 |
| improvement in production and quality of gellan gum by sphingomonas paucimobilis under high dissolved oxygen tension levels. | the effect of agitation rate and dissolved oxygen tension (dot) on growth and gellan production by sphingomonas paucimobilis was studied. higher cell growth of 5.4 g l(-1) was obtained at 700 rpm but maximum gellan (15 g l(-1)) was produced at 500 rpm. dot levels above 20% had no effect on cell growth but gellan yield was increased to 23 g l(-1 )with increase in dot level to 100%. higher dot levels improved the viscosity and molecular weight of the polymer with change in acetate and glycerate co ... | 2006 | 16820976 |
| growth inhibition by metabolites of gamma-hexachlorocyclohexane in sphingobium japonicum ut26. | the growth of a gamma-hexachlorocyclohexane (gamma-hch)-degrading bacterium sphingobium japonicum (formerly sphingomonas paucimobilis) ut26 in rich medium was inhibited by gamma-hch. this growth inhibition was not observed in a mutant that lacked the initial or second step enzymatic activity for gamma-hch degradation, suggesting that metabolites of gamma-hch are toxic to ut26. two metabolites of gamma-hch, 2,5-dichlorophenol (2,5-dcp) and 2,5-dichlorohydroquinone (2,5-dchq), showed a direct toxi ... | 2006 | 16636477 |
| modeling for gellan gum production by sphingomonas paucimobilis atcc 31461 in a simplified medium. | gellan gum production was carried out by sphingomonas paucimobilis atcc 31461 in a simplified medium with a short incubation time, and a kinetic model for understanding, controlling, and optimizing the fermentation process was proposed. the results revealed that glucose was the best carbon source and that the optimal concentration was 30 g liter(-1). as for the fermenting parameters, considerably large amounts of gellan gum were yielded by an 8-h-old culture and a 4% inoculum at 200 rpm on a rot ... | 2006 | 16672479 |
| plasmid-encoded gamma-hexachlorocyclohexane degradation genes and insertion sequences in sphingobium francense (ex-sphingomonas paucimobilis sp+). | the lin genes encode the gamma-hexachlorocyclohexane (gamma-hch or lindane) catabolic pathway in lindane-degrading strains. the location and stability of these genes have been explored in the lindane-degrading sphingobium francense strain sp+, and in two non-lindane-degrading mutants (sp1- and sp2-). the lin genes, lina, linb, line and linx were localized by hybridization on three of the six plasmids of the s. francense strain sp+ showing dispersal within the genome. the linc gene was detected b ... | 2006 | 16553860 |
| expression of glycosylated haloalkane dehalogenase linb in pichia pastoris. | heterologous expression of the bacterial enzyme haloalkane dehalogenase linb from sphingomonas paucimobilis ut26 in methylotrophic yeast pichia pastoris is reported. the haloalkane dehalogenase gene linb was subcloned into the ppiczalphaa vector and integrated into the genome of p. pastoris. the recombinant linb secreted from the yeast was purified to homogeneity and biochemically characterized. the deglycosylation experiment and mass spectrometry measurements showed that the recombinant linb ex ... | 2006 | 16216524 |
| characterization of the novel hch-degrading strain, microbacterium sp. itrc1. | a gram-positive microbacterium sp. strain, itrc1, that was able to degrade the persistent and toxic hexachlorocyclohexane (hch) isomers was isolated and characterized. the itrc1 strain has the capacity to degrade all four major isomers of hch present in both liquid cultures and aged contaminated soil. dna fragments corresponding to the two initial genes involved in gamma-hch degradative pathway, encoding enzymes for gamma-pentachlorocyclohexene hydrolytic dehalogenase (linb) and a 2,5-dichloro-2 ... | 2006 | 16315057 |
| efficient production of 2-pyrone 4,6-dicarboxylic acid as a novel polymer-based material from protocatechuate by microbial function. | sphingomonas paucimobilis syk-6, which can degrade various low molecular weight compounds derived from plant polyphenols such as lignin, lignan, and tannin, metabolizes these substances via 2-pyrone-4,6-dicarboxylic acid (pdc). we focused on this metabolic intermediate as a potential raw material for novel, bio-based polymers. we cloned the ligab and ligc genes of syk-6, which respectively encode protocatechuate 4,5-dioxygenase and 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase, into a ... | 2006 | 16322989 |
| suspected ventilator-associated pneumonia in cardiac patients admitted to the coronary care unit. | to determine the incidence, risk factors, associated pathogens, and outcome of ventilator-associated pneumonia (vap) in patients admitted to a coronary care unit (ccu). | 2006 | 16438476 |
| surveying biotransformations with à la carte genetic traps: translating dehydrochlorination of lindane (gamma-hexachlorocyclohexane) into lacz-based phenotypes. | the ability of the product of a desired reaction to activate a bacterial transcriptional regulator was exploited to develop genetic traps that render the catalytic activity born by a dna clone into a selectable/scorable phenotype. we established this strategy with a system to expose the activity of dehydrochlorinases acting upon gamma-hexachlorocyclohexane (gamma-hch or lindane). to this end, the effector-binding protein, xylr, was evolved by gene shuffling plus mutagenic polymerase chain reacti ... | 2006 | 16478460 |
| distribution of gamma-hexachlorocyclohexane-degrading genes on three replicons in sphingobium japonicum ut26. | sphingobium japonicum (formerly sphingomonas paucimobilis) ut26 utilizes the important insecticide gamma-hexachlorocyclohexane as a sole source of carbon and energy. in previous studies, we isolated and characterized six structural genes (lina to linf) and one regulatory gene (linr) of ut26 for the degradation of gamma-hexachlorocyclohexane to beta-ketoadipate. our analysis in this study indicated that the ut26 genome consists of three large circular replicons of 3.6 mb, 670 kb, and 185 kb. the ... | 2006 | 16487327 |
| microbial contamination of nebulization solution and its measures. | we evaluated the microbial contamination of nebulization solutions in medication cups from a total of 76 ultrasonic nebulizers in use in 10 hospitals. in addition, an interview survey was given to nurses to evaluate the disinfection methods of these ultrasonic nebulizers. of a total of 76 nebulization solution samples, 11 (14.5%) were contaminated with 10-10(2) colony-forming units (cfu)/ml and 9 (11.8%) with 10(3)-10(5) cfu/ml. the major contaminants were glucose non-fermentative bacilli such a ... | 2006 | 16508154 |
| non-specific biodegradation of the organophosphorus pesticides, cadusafos and ethoprophos, by two bacterial isolates. | an enrichment culture technique was used for the isolation of microorganisms responsible for the enhanced biodegradation of the nematicide cadusafos in soils from a potato monoculture area in northern greece. mineral salts medium supplemented with nitrogen (msmn), where cadusafos (10 mg l(-1)) was the sole carbon source, and soil extract medium (sem) were used for the isolation of cadusafos-degrading bacteria. two pure bacterial cultures, named cadi and cadii, were isolated and subsequently char ... | 2005 | 16329956 |
| enantioselective transformation of alpha-hexachlorocyclohexane by the dehydrochlorinases lina1 and lina2 from the soil bacterium sphingomonas paucimobilis b90a. | sphingomonas paucimobilis b90a contains two variants, lina1 and lina2, of a dehydrochlorinase that catalyzes the first and second steps in the metabolism of hexachlorocyclohexanes (r. kumari, s. subudhi, m. suar, g. dhingra, v. raina, c. dogra, s. lal, j. r. van der meer, c. holliger, and r. lal, appl. environ. microbiol. 68:6021-6028, 2002). on the amino acid level, lina1 and lina2 were 88% identical to each other, and lina2 was 100% identical to lina of s. paucimobilis ut26. incubation of chir ... | 2005 | 16332842 |
| [isolation and characterization of a hch degradation sphingomanas sp. stain bhc-a]. | an aerobic bacterium was isolated successfully from a long-term contaminated upland field, which was named bhc-a. the bacterium can utilize hexachlorocycloexane as a sole carbon source and decompose this substance rapidly and completely. according to its physiological & biochemical characters and the homology analysis of its 16s rdna sequence, this strain was identified as sphingomonas sp. stain bhc-a canmineralize not only alpha, gamma and delta-hch rapidly, but also 5 mg/l beta-hch in 12h comp ... | 2005 | 16342765 |
| [metabolic pathway, genes, and enzymes for the degradation of chlorinated pesticide, gamma-hexachlorocyclohexane]. | 2005 | 16218450 | |
| identification and characterization of genes involved in the downstream degradation pathway of gamma-hexachlorocyclohexane in sphingomonas paucimobilis ut26. | sphingomonas paucimobilis ut26 utilizes gamma-hexachlorocyclohexane (gamma-hch) as a sole source of carbon and energy. in our previous study, we cloned and characterized genes that are involved in the conversion of gamma-hch to maleylacetate (ma) via chlorohydroquinone (chq) in ut26. in this study, we identified and characterized an ma reductase gene, designated linf, that is essential for the utilization of gamma-hch in ut26. a gene named lineb, whose deduced product showed significant identity ... | 2005 | 15659662 |
| identification of insertion sequence from a gamma-hexachlorocyclohexane degrading bacterium, sphingomonas paucimobilis ut26. | tn5-derived mutants of the gamma-hexachlorocyclohexane-degrading bacterium sphingomonas paucimobilis ut26 were genetically characterized, and an endogenous insertion sequence (is) which belongs to the is1380 family was identified. the is, named issp1, existed as multi copies in ut26, and its transposition appeared to be activated during the process of tn5-mutagenesis. it was found that transposon mutagenesis can cause endogenous mutations. | 2005 | 15665490 |
| quantitative analysis of substrate specificity of haloalkane dehalogenase linb from sphingomonas paucimobilis ut26. | haloalkane dehalogenases are microbial enzymes that cleave a carbon-halogen bond in halogenated compounds. the haloalkane dehalogenase linb, isolated from sphingomonas paucimobilis ut26, is a broad-specificity enzyme. fifty-five halogenated aliphatic and cyclic hydrocarbons were tested for dehalogenation with the linb enzyme. the compounds for testing were systematically selected using a statistical experimental design. steady-state kinetic constants k(m) and k(cat) were determined for 25 substr ... | 2005 | 15736949 |
| a tetrahydrofolate-dependent o-demethylase, ligm, is crucial for catabolism of vanillate and syringate in sphingomonas paucimobilis syk-6. | vanillate and syringate are converted into protocatechuate (pca) and 3-o-methylgallate (3mga), respectively, by o-demethylases in sphingomonas paucimobilis syk-6. pca is further degraded via the pca 4,5-cleavage pathway, while 3mga is degraded through multiple pathways in which pca 4,5-dioxygenase (ligab), 3mga 3,4-dioxygenase (desz), and an unidentified 3mga o-demethylase and gallate dioxygenase are participants. for this study, we isolated a 4.7-kb smai fragment that conferred on escherichia c ... | 2005 | 15743951 |
| degradation of beta-hexachlorocyclohexane by haloalkane dehalogenase linb from sphingomonas paucimobilis ut26. | beta-hexachlorocyclohexane (beta-hch) is the most recalcitrant among the alpha-, beta-, gamma-, and delta-isomers of hch and causes serious environmental pollution problems. we demonstrate here that the haloalkane dehalogenase linb, reported earlier to mediate the second step in the degradation of gamma-hch in sphingomonas paucimobilis ut26, metabolizes beta-hch to produce 2,3,4,5,6-pentachlorocyclohexanol. | 2005 | 15812056 |
| osteomyelitis and secondary septic arthritis caused by sphingomonas paucimobilis. | we describe a case of osteomyelitis and secondary septic arthritis caused by sphingomonas paucimobilis in an immunosupressed adolescent which did not present typically, leading to a delay in diagnosis. this case highlights the need to thoroughly investigate musculoskeletal pain in the immunocompromised patient in order to exclude an infection as a cause. | 2005 | 15827879 |
| isolation and characterization of phenanthrene-degrading sphingomonas paucimobilis strain zx4. | phenanthrene-degrading bacterium strain zx4 was isolated from an oil-contaminated soil, and identified as sphingomonas paucimobilis based on 16s rdna sequence, cellular fatty acid composition, mol% g + c and biolog-gn tests. besides phenanthrene, strain zx4 could also utilize naphthalene, fluorene and other aromatic compounds. the growth on salicylic acid and catechol showed that the strain degraded phenanthrene via salicylate pathway, while the assay of catechol 2,3-dioxygenase revealed catecho ... | 2005 | 15865153 |
| cell wall glycosphingolipids of sphingomonas paucimobilis are cd1d-specific ligands for nkt cells. | the current consensus on characterization of nkt cells is based on their reactivity to the synthetic glycolipid, alpha-galactosylceramide (alpha-galcer) in a cd1d-dependent manner. because of the limited availability of alpha-galcer, there is a constant search for cd1d-presented ligands that activate nkt cells. the alpha-anomericity of the carbohydrate is considered to be an important requisite for the cd1d-specific activation of nkt cells. the gram-negative, lipopolysaccharide-free bacterium sp ... | 2005 | 15915536 |
| cloning, sequence analysis, and expression of the gene encoding sphingomonas paucimobilis fp2001 alpha-l -rhamnosidase. | the gene (rham) encoding the alpha-l-rhamnosidase of sphingomonas paucimobilis fp2001 was cloned, sequenced, and expressed in escherichia coli. the rham consisted of 3,354 nucleotides and had a promoter and shine-dalgarno sequences typical in bacteria. the rham encoding a protein (rham) deducted from the sequence consisted of 1,117 amino acids and had a putative signal peptide of 25 amino acids. rham has no similarity to other known rhamnosidases. rham has a sugar-binding domain of glycoside hyd ... | 2005 | 15991055 |
| ['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp]. | gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ... | 2005 | 15991478 |
| molecular characterization of the gallate dioxygenase from pseudomonas putida kt2440. the prototype of a new subgroup of extradiol dioxygenases. | in this work we have characterized the gala gene product from pseudomonas putida kt2440, a ring-cleavage dioxygenase that acts specifically on gallate to produce 4-oxalomesaconate. the protein is a trimer composed by three identical subunits of 47.6 kda (419 amino acids) that uses fe2+ as the main cofactor. the gallate dioxygenase showed maximum activity at ph 7.0, and the km and vmax values for gallate were 144 microm and 53.2 micromol/min/mg of protein, respectively. a phylogenetic study sugge ... | 2005 | 16030014 |
| characterization of the gallate dioxygenase gene: three distinct ring cleavage dioxygenases are involved in syringate degradation by sphingomonas paucimobilis syk-6. | sphingomonas paucimobilis syk-6 converts vanillate and syringate to protocatechuate (pca) and 3-o-methylgallate (3mga) in reactions with the tetrahydrofolate-dependent o-demethylases ligm and desa, respectively. pca is further degraded via the pca 4,5-cleavage pathway, whereas 3mga is metabolized via three distinct pathways in which pca 4,5-dioxygenase (ligab), 3mga 3,4-dioxygenase (desz), and 3mga o-demethylase (ligm) are involved. in the 3mga o-demethylation pathway, ligm converts 3mga to gall ... | 2005 | 16030198 |
| 16s rdna phylogeny and distribution of lin genes in novel hexachlorocyclohexane-degrading sphingomonas strains. | hexachlorocyclohexane (hch) is a highly recalcitrant pesticide that persists in soils. three novel hch-degrading strains (ds2, ds2-2 and ds3-1) were isolated after enrichment from hch-contaminated soil from germany. these strains efficiently degraded the alpha-, gamma- and delta-isomers of hch, while strain ds3-1 also degraded beta-hch. based on 16s rdna analysis, strain ds3-1 was closely related to sphingomonas taejonensis, while strains ds2 and ds2-2 were closely related to sphingomonas flava ... | 2005 | 16104856 |
| a second 5-carboxyvanillate decarboxylase gene, ligw2, is important for lignin-related biphenyl catabolism in sphingomonas paucimobilis syk-6. | a lignin-related biphenyl compound, 5,5'-dehydrodivanillate (ddva), is degraded to 5-carboxyvanillate (5cva) by the enzyme reactions catalyzed by ddva o-demethylase (ligx), meta-cleavage oxygenase (ligz), and meta-cleavage compound hydrolase (ligy) in sphingomonas paucimobilis syk-6. 5cva is then transformed to vanillate by a nonoxidative 5cva decarboxylase and is further degraded through the protocatechuate 4,5-cleavage pathway. a 5cva decarboxylase gene, ligw, was isolated from syk-6 (x. peng, ... | 2005 | 16151081 |
| hexachlorocyclohexane-degrading bacterial strains sphingomonas paucimobilis b90a, ut26 and sp+, having similar lin genes, represent three distinct species, sphingobium indicum sp. nov., sphingobium japonicum sp. nov. and sphingobium francense sp. nov., and reclassification of [sphingomonas] chungbukensis as sphingobium chungbukense comb. nov. | three strains of sphingomonas paucimobilis, b90a, ut26 and sp+, isolated from different geographical locations, were found to degrade hexachlorocyclohexane. phylogenetic analysis based on 16s rrna gene sequences indicated that these strains do not fall in a clade that includes the type strain, sphingomonas paucimobilis atcc 29837(t), but form a coherent cluster with [sphingomonas] chungbukensis imsnu 11152(t) followed by sphingobium chlorophenolicum atcc 33790(t). the three strains showed low dn ... | 2005 | 16166696 |
| [screening methods for detection of metallo-beta-lactamase producing gram negative rods]. | modified hodge test and a method using a disk with imipenem plus 1000 mg of edta were used to determine the presence of metallo-beta-lactamase producing gram-negative rods among 166 clinical isolates from hospitals in iaşi and galaţi. of 9 imipenem resistant strains found, only one pseudomonas aeruginosa gave positive results with both tests and other two p. aeruginosa clinical isolates gave negative results with both tests. the rest of the strains (2 p. aeruginosa, 2 acinetobacter baumanii, 1 s ... | 2005 | 16607806 |
| [rare bacteria species found in wounds of tsunami patients. predominance of gram-negative rods, increased antibiotic resistance]. | microbiological cultures from 229 patients seeking medical advise in stockholm after the tsunami catastrophe december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative rods were the most common findings from wound cultures. common human pathogens as escherichia coli, proteus species, klebsiella spp, and pseudomonas aeruginosa were isolated. however, more rare species of gram-negative rods were also isolated, e.g. myroides ... | 2005 | 16416946 |
| characteristics of bacterial strains inhabiting the wood of coniferous trees. | the presented studies embraced samples of wood chips from coniferous trees which contained layers of duramen, alburnum and bark. microbiological analysis involved qualitative and quantitative determination of bacterial flora inhabiting the studied wood material. the wood chips were found to contain primarily species belonging to the genera bacillus and pseudomonas. the presence of the potentially pathogenic species bacillus cereus 1, sphingomonas paucimobilis, aeromonas salmonicida and chryseomo ... | 2004 | 15790079 |
| three-block bi-focal pls (3bif-pls) and its application in qsar. | when x and y are multivariate, the two-block partial least squares (pls) method is often used. in this paper, we outline an extension addressing a special case of the three-block (x/y/z) problem, where z sits "under" y. we have called this approach three-block bi-focal pls (3bif-pls). it views the x/y relationship as the dominant problem, and seeks to use the additional information in z in order to improve the interpretation of the y-part of the x/y association. two data sets are used to illustr ... | 2004 | 15669704 |
| crystal structure of haloalkane dehalogenase linb from sphingomonas paucimobilis ut26 at 0.95 a resolution: dynamics of catalytic residues. | we present the structure of linb, a 33-kda haloalkane dehalogenase from sphingomonas paucimobilis ut26, at 0.95 a resolution. the data have allowed us to directly observe the anisotropic motions of the catalytic residues. in particular, the side-chain of the catalytic nucleophile, asp108, displays a high degree of disorder. it has been modeled in two conformations, one similar to that observed previously (conformation a) and one strained (conformation b) that approached the catalytic base (his27 ... | 2004 | 14744129 |
| reactions of serine palmitoyltransferase with serine and molecular mechanisms of the actions of serine derivatives as inhibitors. | serine palmitoyltransferase (spt) is a key enzyme in sphingolipid biosynthesis and catalyzes the decarboxylative condensation of l-serine and palmitoyl coenzyme a to 3-ketodihydrosphingosine. we have succeeded in the overproduction of a water-soluble homodimeric spt from sphingomonas paucimobilis ey2395(t) in escherichia coli. the recombinant spt showed the characteristic absorption and circular dichroism spectra derived from its coenzyme pyridoxal 5'-phosphate. on the basis of the spectral chan ... | 2004 | 14744154 |