Publications

immunochemical identification of antigens of brucella melitensis by means of crie.	a crude extract of brucella melitensis was obtained by sonication, centrifugation and dialysis, and analyzed by quantitative immunoelectrophoresis. cie analysis, using a high titer purified rabbit antibody anti-brucella fraction, showed that the brucella melitensis extract contained at least 22 antigens that all moved towards the anode. the antigens of the crude extract able to interact with igg of patients affected by brucellosis, were identified by means of crie using sheep 125i-anti human igg ...	1987	3587065
prosthetic valve endocarditis caused by brucella melitensis. a report of four cases successfully treated with tetracycline, streptomycin, and sulfamethoxazole and trimethoprim plus valve replacement.	optimal treatment of prosthetic valve endocarditis due to brucella melitensis is unknown. the presence of ring abscess makes extensive surgical débridement and valve replacement essential steps of management. antimicrobial therapy with tetracycline hydrochloride plus streptomycin sulfate or sulfamethoxazole and trimethoprim can achieve the sterilization of infected cardiac tissue. late bland periprosthetic leaks occur frequently. reoperation can be safely performed after a period of antimicrobia ...	1987	3592879
sacroiliitis as a sole manifestation of brucella melitensis infection in a child.	a case of a 12-year-old boy with sacroiliitis documented by positive tc-99m mdp and ga-67 scans is described. isolation of brucella melitensis from the blood and bone marrow established the diagnosis. he responded promptly to docycycline therapy. throughout the course of his disease this boy had neither fever nor other signs of brucellosis, and x-ray was normal.	1987	3595029
brucella melitensis meningitis following head trauma.		1987	3596810
brucellar arthritis: a study of 39 peruvian families.	a study was conducted to characterise the articular manifestation of brucella melitensis within a family in peru. from january 1981 to june 1986, 39 families with 232 individuals were evaluated. brucellosis was diagnosed in 118 family members (attack rate of 50.9%). a lower attack rate was observed in children less than 10 years' old compared with other age groups (p less than 0.02). complete clinical data were available in 92 of the 118 affected members. moderate and severe forms of the disease ...	1987	3662637
[a study of identification of two atypical strains of brucella melitensis].		1987	3664616
infective aortitis due to brucella melitensis.	infective aneurysms caused by brucella sp. are extremely rare. b. suis and b. abortus have been implicated in a few cases but to our knowledge, b. melitensis has not been reported as a cause of mycotic aneurysm of the abdominal aorta. we here report the first case of this described in the english literature. the patient was successfully treated with resection of the infected aneurysm, placement of an axillo-bifemoral graft, and prolonged antibiotic treatment (doxycycline and streptomycin). exten ...	1987	3672053
immunization with brucella melitensis rev 1 against brucella ovis infection of rams.	the efficacy of brucella melitensis rev 1 vaccine (rev 1) for the prophylaxis of brucella ovis ram epididymitis was evaluated. twenty-nine 3-month-old rams were vaccinated with 2 x 10(9) rev 1 and 14 were revaccinated with 5 x 10(8) at 14 months of age. six rams remained unvaccinated as a control group. all rams were challenged with 5 x 10(8) b. ovis at 21 months of age. before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams deve ...	1987	3686824
cryoglobulinemia and cutaneous vasculitis in human brucellosis.	we report the occurrence of cryoglobulinemia and cutaneous vasculitis in three patients with brucellosis caused by brucella melitensis. the isolated cryoglobulins were characterized as mixed polyclonal or type iii. brucella agglutinin activity was not detected in any of the cryoglobulins analyzed. however, the same agglutinin titer and the presence of precipitin lines of identity in immunodiffusion gels were observed in the supernatant of the cryoglobulins and the serum. rheumatoid-factor activi ...	1987	3693520
structural elucidation of the brucella melitensis m antigen by high-resolution nmr at 500 mhz.	the brucella m antigen from the species type strain brucella melitensis 16m has been identified as a component of the cell wall lipopolysaccharide (lps). o polysaccharide liberated from this lps by mild acid hydrolysis exhibited m activity in serological tests and was shown to be a homopolymer of 4-formamido-4,6-dideoxy-alpha-d-mannopyranosyl residues arranged in an oligosaccharide repeating unit as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the native lipopolysacchar ...	1987	3442684
serological screenings of various infectious diseases on the cape verde islands (west africa).	general screening investigations with various antigens were carried out with a view to further specific investigations being carried out on the cape verde islands concerning infectious diseases. serological positive reactions were found in mumps, adeno, plt, cytomegaly, herpes, para-influenza 1, 2, 3, influenza a and b, mycoplasmosis, rs-virus, gonorrhoea, hepatitis a and b, r. conori, malaria, syphilis, brucella abortus, brucella melitensis, varicella, legionella, picornavirus, measles, german ...	1987	3443744
serological investigations in nigeria for anthropozoonoses in human sera: brucellosis, echinococcosis, toxoplasmosis, chlamydial diseases, listeriosis, rickettsiosis (coxiella burneti and rickettsia conori).	176 blood sera taken from patients in the hospital of minna and abeokuta (nigeria) were examined for anthropozoonoses. the following positive reactions could be found: brucella abortus 9%, brucella melitensis 11.7%, echinococcosis 0.53%, toxoplasmosis 79.2% (cf-test 6.9%), chlamydial diseases 45%, listeriosis 28.7% (typ 1h) and 19.7% (typ 4bh), rickettsiosis--rickettsia conori 18.6% and coxiella burneti 63.3%.	1987	3443751
conjunctival vaccination of young goats with brucella melitensis strain rev 1.	three groups each of 15 four-month old female kids were vaccinated with brucella melitensis strain rev 1 either conjunctivally (1.1 x 10(7) or 1.1 x 10(9) cfu) or subcutaneously (1.1 x 10(9) cfu). one group of 15 kids remained unvaccinated as control. all kids were mated when 9.5 month old. three months later, they were challenged conjunctivally with b melitensis strain h38 at a dose of 5.1 x 10(6) cfu. blood samples collected before vaccination and throughout the experiment were subjected to ro ...	1987	3451685
brucella meningitis.	infection with brucella melitensis is endemic in saudi arabia but involvement of the central nervous system (cns) is rare. we report on three patients with acute brucella meningitis, all of whom had a history of exposure to a possible source of infection. diagnosis was confirmed by isolation of brucella species from blood cultures. examination of cerebrospinal fluid revealed lymphocytic pleocytosis with a high concentration of protein and low concentration of glucose. the patients were treated b ...	1987	3494790
osteoarticular complications of brucellosis: a study of 169 cases.	of 452 patients with brucellosis, 169 (111 male and 58 female) had osteoarticular complications. brucella melitensis was isolated from the blood in 7.7% of the cases. fever, chills, arthralgia, backache, high levels of c-reactive protein, positive rheumatoid factor, and splenomegaly were more frequent in osteoarticular brucellosis than in nonosteoarticular disease. arthritis occurred in the hip joint in 90 cases (53%), knees in 61 (36%), sacroiliacs in 33 (20%), ankles in 25 (15%), elbows in nin ...	1987	3496650
influence of autoclaved brucella melitensis on the proliferation of human lymphocytes.	brucella melitensis is a facultative intracellular bacterium which binds spontaneously to human b-lymphocytes. in order to find out whether there exists a relation between bacterial adherence and lymphocyte reactivity, the proliferation response of normal adult peripheral blood lymphocytes to autoclaved b. melitensis has been studied. our results indicate that autoclaved b. melitensis induce only a slight increase in proliferation. this low lymphocyte response is due to proliferating b-cells. in ...	1987	3499149
control of brucella melitensis brucellosis in developing countries.		1987	3300717
control of brucella melitensis infection in sheep and goats--a review.		1987	3307078
risk of infection by brucella melitensis for people living near infected goats.	taking advantage of a local outbreak of brucellosis in a herd of goats, we have assessed the risk of infection for people living nearby. by means of serological tests we have shown that such a risk is small provided that direct contact with contaminated tissues or secretions of goats is avoided.	1987	3312421
[endocarditis with unusual causative agents].	in the period 1947-1985, 601 patients with infective endocarditis were seen at the university hospital zurich and the kantonsspital lucerne. streptococci, enterococci and staphylococci were the predominant causative organisms in two-thirds of all cases. in more than 25% of the patients blood cultures remained negative. in 6 patients endocarditis was caused by very rare organisms, viz. coxiella burnetii (2 cases), hemophilus parainfluenzae, corynebacterium bovis (diphtheroids), brucella melitensi ...	1987	3324331
release of outer membrane fragments by exponentially growing brucella melitensis cells.	rough and smooth strains of brucella melitensis released a membranous material that was devoid of detectable nadh oxidase and succinic dehydrogenase activity (cytoplasmic membrane markers) but that contained lipopolysaccharide, proteins, and phospholipids. this material was composed of two fractions that had similar chemical compositions but that were of different sizes which were separated by differential ultracentrifugation. electron microscopy showed that both fractions are made of unit membr ...	1987	3818086
the spectrum of pneumonia in 1983 at the riyadh armed forces hospital.	the agents causing pneumonia have been assessed in 112 adult patients admitted to the armed forces hospital in riyadh during a period of one year: pathogens were identified in 78 patients (69.6%). sputum culture produced a significant isolate in 60 patients (53.5%), and in 17 (15.2%) the causative agent was suggested by serological tests. streptococcus pneumonia was the commonest infecting agent (21.4%). pneumonia due to mycobacterium tuberculosis was diagnosed in eight patients, to mycoplasma p ...	1987	3819456
an evaluation of diagnostic methods for brucellosis--the value of bone marrow culture.	fifty patients, eventually diagnosed as having brucellosis, were studied prospectively for comparison of a simple culture of bone marrow aspirate from the iliac crest (0.5-1.0 ml) with two cultures of blood (5-10 ml) taken 30-60 min apart and with a tube-agglutination test. cultures of bone marrow and blood were positive in 92% and 70% of the patients, respectively (p less than .001). bacteria multiplied significantly faster in bone marrow cultures (4.32 vs. 6.65 days; p less than .001). all iso ...	1986	3941276
[lumbosacral meningoradiculitis as a late manifestation of brucellosis (brucella melitensis)].	neurologic complications can occur during the course of brucellosis. in a 25-year-old patient admitted july 3, 1984 because of loss of weight (10 kg) brucellosis (brucella melitensis) was diagnosed on the basis of splenomegaly, positive agglutination and positive blood and bone marrow cultures. therapy with cotrimazol was performed for eight weeks. on july 31, 1984 the patient was readmitted because of weakness in both legs. neurologic examination revealed slight weakness of the hip extensors an ...	1986	3952482
inhibition of in vitro immunoglobulin synthesis by brucella melitensis.		1986	3502547
[in vitro erythrophagocytosis in detecting opsonizing antibodies in a patient with malta fever. a hypothesis on the explanation of in vivo hemophagocytosis in other patients with malta fever].	in vitro agglutination and phagocytosis of erythrocytes by homologous neutrophil granulocytes and monocytes could be observed using the blood of a healthy person (blood group a, rh+) to detect opsonising antibodies in the serum of a patient with malta fever (blood group o, rh+). agglutination and erythrophagocytosis were independent of complement or the presence of brucella melitensis. in patients with malta fever and blood groups o and b, respectively, antibodies could be detected on the surfac ...	1986	3519436
brucellosis--cause of abortion in sheep and its public health significance.	present study was undertaken to determine the association of brucellosis with abortions occurring naturally in sheep at an organized local sheep breeding farm. a total of 15 strains of brucella melitensis biovar i were isolated from the abortion material. serologically the aborted ewes were positive for brucellosis by one or more tests. during acute infection (abortion), standard tube agglutination test (sat) detected more positive reactors (70.7%) while counter immunoelectrophoresis (cie) detec ...	1986	3557828
intracellular killing of brucella melitensis within mouse peritoneal macrophages: influence of treatment with rifampicin. an ultrastructural study.	the aggregation and condensation of ribosomes and the disjunction of the cell-wall membranous system are the ultrastructural alterations caused by rifampicin on b. melitensis cultured in brucella-broth medium. our ultrastructural researches carried out on mouse peritoneal macrophages infected with b. melitensis and treated with rifampicin (1 microgram/ml) have demonstrated that vacuoles containing b. melitensis which had been damaged by the drug fuse with lysosomes. on the contrary vacuoles cont ...	1986	3713542
demonstration of antibodies against brucella melitensis 16m lipopolysaccharide and native hapten in human sera by enzyme-linked immunosorbent assay.	an enzyme-linked immunosorbent assay (elisa) for the detection and quantification of human immunoglobulin g (igg), iga, and igm antibodies against brucella melitensis 16m by using lipopolysaccharide (lps) and native hapten (nh) as antigens is described. the results obtained with the lps elisa were compared with the results of the nh elisa. a good statistically significant correlation was established between the antibody titers of the igg class against both antigens. a total of 104 (99%) of the 1 ...	1986	3722367
in vitro susceptibility of brucella melitensis to new cephalosporins crossing the blood-brain barrier.	the in vitro susceptibilities of 83 clinical isolates of brucella melitensis to seven cephalosporins and a monobactam were determined. ceftizoxime, ceftriaxone, and cefotaxime were the most effective agents tested, with mics ranging from 0.25 to 2 micrograms/ml. moxalactam, cefoperazone, cefuroxime, and ceftazidime showed mics between 4 and 64 micrograms/ml, with moxalactam being the most active agent in this group. aztreonam showed poor activity, with mics higher than 64 micrograms/ml.	1986	3729331
cardiac manifestations in brucellosis.	cardiac involvement in childhood brucellosis is rare and when present mimics findings usually noted in acute rheumatic fever with carditis. we report five children aged 6 to 11 years. echocardiography showed mitral valve vegetations in one, functional mitral valve incompetence in two, and sluggish myocardial function in one. all the patients presented with fever, arthralgia, and malaise, four of them had leucopenia, and all five showed relative lymphocytosis. blood cultures grew brucella meliten ...	1986	3729526
brucella arthritis in children.	a study in 50 children suffering from acute brucellosis demonstrated that acute arthritis is a common and often predominant manifestation of the disease. the patients lived in an area where brucellosis is endemic: their ages ranged from six months to 12 years. all patients had a brucella agglutination titer of greater than or equal to 1:320 on admission. the knee and hip joints were most commonly affected with symptoms and findings from only one joint predominating. associated findings of only m ...	1986	3793238
evaluation of elisa in the diagnosis of acute and chronic brucellosis in human beings.	an enzyme-linked immunosorbent assay (elisa) was used to determine the levels of brucella-specific igg, igm and iga in 173 patients with acute brucellosis, 22 patients with chronic brucellosis and in 281 controls consisting of 98 patients with other infectious etiologies, 20 patients with non-infectious diseases and 163 normal healthy adults. the elisa results were compared with culture findings, the results of slide agglutination tests with brucella melitensis (m), b. abortus (a) and ross benga ...	1986	3794323
urban outbreak of goat cheese brucellosis.	in 1983 an outbreak of human brucellosis caused by brucella melitensis occurred among residents of a predominantly hispanic neighborhood in houston, tx. the source of the infections was traced to unpasteurized goats' milk cheese imported from mexico. nineteen of the 31 patients who contracted the disease were members of 5 families, which included 11 children ranging in age from 2 to 17 years. infection was generally associated with protein complaints and a paucity of physical findings. both immu ...	1986	3797296
rifampicin resistance in a strain of brucella melitensis after treatment with doxycycline and rifampicin.		1986	3804888
relevance of in vitro antimicrobial susceptibility of brucella melitensis to relapse rate in human brucellosis.	the in vitro susceptibility of brucella melitensis was examined vis-a-vis the clinical outcome in 75 patients with brucellosis. the initial mics for brucella isolates from patients who relapsed and from those who did not were similar. furthermore, the mics for isolates from patients whose infections relapsed were no different from those for original isolates. our results clearly showed that neither initial nor subsequent antibiotic susceptibility plays a role in the likelihood of relapse of pati ...	1986	3813520
evaluation of blood clot cultures for isolation of salmonella typhi, salmonella paratyphi-a, and brucella melitensis.	two types of clot culture, one with taurocholate-streptokinase and the other with bile as a culture medium, and two conventional cultures of whole blood were evaluated in parallel in an area where typhoid fever and brucellosis are endemic. each of the four systems contained 5 ml of blood or the clot derived from 5 ml of blood and sufficient broth to yield a 1:11 dilution of the specimen. of 542 patients studied, salmonella paratyphi-a was isolated from 61, s. typhi from 46, and brucella melitens ...	1986	3093527
enzyme-linked immunosorbent assay with major outer membrane proteins of brucella melitensis to measure immune response to brucella species.	we developed an enzyme-linked immunosorbent assay (elisa) system to measure human immunoglobulin g (igg) and igm response to the major outer membrane proteins of brucella melitensis. the elisa was more sensitive in detecting antibody than a standard microagglutination (ma) test with b. abortus antigen. of 101 sera from persons with suspected brucellosis, 79 (78.2%) gave elisa igm titers greater than or equal to the b. abortus ma titer without 2-mercaptoethanol (2me), which measures both igm and ...	1986	3095364
antigenic differences between brucella abortus and brucella melitensis recognized by a monoclonal antibody.	by means of somatic hybridization of spleen cells from balb/c mice inoculated with brucella abortus and of a line of murine myeloma, a monoclone was obtained producing an antibody which specifically recognizes an epitope of brucella abortus by means of the agglutination reaction. this epitope is not only on the surface of the homologous strains but on all the brucella abortus biotype i, on the brucella melitensis biotype ii and on the brucella suis biotype ii tested. on the contrary it is not pr ...	1986	2423851
in-vitro activity of ciprofloxacin, ceftriaxone and five other antimicrobial agents against 95 strains of brucella melitensis.	we studied the in-vitro activity of seven antibiotics against 95 strains of brucella melitensis isolated in blood cultures of 95 patients with brucellosis. the minimum inhibitory concentration (mic) was measured by the agar dilution method. all strains of b. melitensis were inhibited by doxycycline at 0.25 mg/l, tetracycline at 0.5 mg/l, ciprofloxacin at 0.5 mg/l, streptomycin at 1 mg/l, ceftriaxone at 1 mg/l, rifampicin at 4 mg/l and by co-trimoxazole at 0.5/9.5 mg/l. we did not find strains re ...	1986	2940209
alimentary tract presentations of brucellosis.	a retrospective study was conducted to assess alimentary tract involvement in 140 saudi patients with acute or chronic brucellosis. a brucella standard agglutination titer of 160 or over was considered to be positive evidence of brucella infection. the commonest symptoms were anorexia, weight loss, right hypochondrial pain, and constipation. a history of raw milk ingestion was obtained in 38 patients. thirty-nine patients had hepatomegaly, 21 had splenomegaly, and six had signs of cholecystitis. ...	1986	21164236
[somatic antigens of the brucella genus. the structure of the o-specific polysaccharide chain of brucella melitensis lipopolysaccharide].	the phenol-phase soluble antigenic lipopolysaccharide was isolated from brucella melitensis, strain 565, by the routine phenol/water procedure followed by chromatography on sepharose 4b. after mild acid hydrolysis and chromatography on sephadex g-50, the lipopolysaccharide yielded a linear o-specific polysaccharide built up from 1,2-linked 4,6-dideoxy-4-formamido-alpha-d-mannopyranosyl units. the structure of the polysaccharide was deduced mainly from the nuclear magnetic resonance and methylati ...	1985	2413867
neurobrucellosis in an eight-year-old child.	an 8-year-old boy, presenting with signs of chronic meningitis, and involvement of cranial nerves, was found to have neurobrucellosis. the diagnosis was rapidly established by an enzyme-linked immunosorbent assay (elisa) that detected high titres of igg, iga and igm brucella antibodies in csf and in serum. brucella melitensis was subsequently isolated both from blood and csf. neurobrucellosis, which has been considered to be very rare in childhood, should be suspected in cases of meningitis of o ...	1985	2418766
brucella melitensis pericarditis.		1985	3993521
survey of brucellosis in goats and sheep in the yemen arab republic: comparison of tests for brucella melitensis infection in sheep.	sera from 538 yemeni goats and 690 yemeni sheep were screened for brucellosis by the rose bengal plate test (rbpt) and reactors confirmed by the complement fixation test (cft) and the serum agglutination test (sat). the prevalence among goats was 0.4% and among sheep 0.6%. the prevalence among 183 imported goats and sheep was 4.4%. the sensitivity and specificity of three serological tests available for the diagnosis of brucellosis -cft, rbpt and sat - were compared using ovine sera obtained thr ...	1985	4024267
isolation of brucella melitensis from sheep in syria.		1985	4060527
comparative trial of rifampin-doxycycline versus tetracycline-streptomycin in the therapy of human brucellosis.	in an attempt to compare the efficacy of rifampin-doxycycline with tetracycline-streptomycin for the treatment of human brucellosis, we administered both combinations for a 30-day period, similar to the period recommended by the world health organization in a prospective, randomized trial. forty-six patients were included in the final study (36 men and 10 women); 41 had blood cultures positive for brucella melitensis. the 28 patients in group a received tetracycline hydrochloride at doses of 0.5 ...	1985	4073878
humoral immune abnormalities in human brucellosis.	humoral immune responses were evaluated in a group of patients with acute brucellosis due to brucella melitensis biotype i. levels of immunoglobulins, c3 and c4, and the presence of antinuclear antibodies, rheumatoid factor and circulating immune complexes were sequentially studied in all brucellosis patients during the acute phase of the disease and following specific therapy. elevated levels of all immunoglobulin classes, igg, igm and iga and c3, c4, were found in the majority of patients. ant ...	1985	4083235
vaccination of ewes by a single conjunctival administration of brucella melitensis rev. 1 vaccine.	in a lot of 99 ewes, the efficacy of a single vaccination by the conjunctival route (vc) with either 1.4 x 10(8) or 1.4 x 10(9) cfu brucella melitensis rev. 1 was compared with that of normal subcutaneous (sc) vaccination with 1.6 x 10(9) cfu. the ewes were challenged seven months after vaccination, when 85 days pregnant, with 5 x 10(7) cfu brucella melitensis strain h38 administered by conjunctival route. complement fixation, rose bengal and allergic tests were performed at intervals between va ...	1985	4091489
brucellosis: serological methods compared.	at least 12 persons contracted clinical, and 4 persons subclinical brucella melitensis infection during a brucellosis epidemic in the spring and summer of 1983 in southern germany, a region which had been free of this disease for the past 20 years. all cases of illness were traced to one infected herd of sheep. the presence of antibodies against b. melitensis was examined in 72 sera of infected patients using the following tests: agglutination, coomb's test, two complement fixation tests with di ...	1985	4093607
ovine brucellosis: repeated vaccination with rev 1 vaccine and the prevalence of the disease in the winburg district.	the effect of repeated vaccination of ram lambs with brucella melitensis rev 1 vaccine was studied in relation to complement fixation titres. the presence of brucella organisms in semen and changes in neutrophil densities of the semen were also studied. a survey of the incidence of ovine brucellosis in the winburg district on the basis of a clinical examination of rams and the complement fixation test is also reported on. repeated vaccination of ram lambs with rev 1 vaccine resulted in high comp ...	1985	3836306
brucella fractions behave as nonspecific mitogens and polyclonal b-cell activators for human lymphocytes.	two lipid-a-free fractions which were extracted from brucella melitensis and were designated pi and sf stimulated human unsensitized mononuclear cells to proliferate and to secrete immunoglobulins. both of these effects were observed in cultures of peripheral blood, tonsils, and cord blood lymphocytes. neither b cells nor t cells alone proliferated in the presence of these fractions, whereas the proliferative response of t cells plus b cells was largely independent of accessory cells. polyclonal ...	1985	3876286
[general mathematical methodology for analyzing bacterial biotope-biotype correspondence: application to the epidemiology of brucella melitensis in france].		1985	3879674
brucellar spondylitis: a detailed analysis based on current findings.	three hundred thirty-one cases of brucellosis included in a 10-year prospective protocol were reviewed to identify and follow up patients with spondylitis. of 20 patients (17 male and three female; mean age, 54 years), spondylitis was diagnosed soon after onset of the brucellosis in 15, there were significant systemic symptoms in 17, and blood cultures were positive for brucella melitensis in 14. the main symptom was vertebral pain. the commonest radiographic changes were narrowing of the disk a ...	1985	3903944
characterization of monoclonal antibodies against brucella melitensis.		1985	3907209
phagocytosis and killing of brucella by human polymorphonuclear leukocytes.	although cellular immunity involving activated macrophages is important in resistance to brucella, serum factors and polymorphonuclear leukocytes (pmnls) play some role in the initial response to infection. the interaction between human pmnls and virulent and attenuated strains of brucella abortus and brucella melitensis was studied by in vitro techniques. virulent and attenuated strains of both species were rapidly phagocytosed after opsonization with normal human serum (nhs); nonopsonized bact ...	1985	3919118
brucellosis in a group of travelers to spain.	an epidemiologic investigation, initiated when brucella melitensis infection was detected in a high school student, identified five unrecognized cases in classmates. before the investigation, four infected students had symptoms of brucellosis for one to ten weeks, made nine visits to physicians, and were confined to the school infirmary or hospitals for 27 days. the other two students were asymptomatic when brucella agglutination testing demonstrated elevated titers, and treatment was quickly in ...	1984	6690819
[rifampin in osteoarthritis due to brucella melitensis].		1984	6714826
[brucellosis: a varied clinical presentation in 9 patients].	from 1973 to 1983 nine cases of brucella melitensis infection were hospitalised at the centre hospitalier universitaire vaudois (chuv), lausanne. in each case, the infection was acquired in a mediterranean country (4 cases in italy, 2 in spain, 2 in portugal and 1 in greece). in 6 of the cases the disease was acquired by ingestion of dairy products and in 2 cases by direct animal contact. despite classical initial symptomatology (fever, rigors, weakness), the time from first symptoms to diagnosi ...	1984	6335295
non-specific stimulation of human b lymphocytes by a brucella fraction.	pi, a lipid a-free fraction (15) extracted from the cell wall of brucella melitensis type i m.15 behaves as a non specific mitogen and a polyclonal b-cell activator (pba) for human unsensitized lymphocytes from subjects with no previous history and no biological stigmata of brucellosis. both effects may be evidenced, indifferently, in cultures of peripheral blood, tonsils or cord blood lymphocytes. mitogenicity, estimated by the incorporation of tritiated thymidine is maximal on the 5th and 6th ...	1984	6208072
[identification and taxonomy of brucella melitensis by factor analysis of the relations and automatic classification].		1984	6386576
[discrimination of taxa of the species brucella melitensis and the determination of strain types].		1984	6386577
comparison of phenol- and heat-killed antigens in the indirect immunofluorescence test for serodiagnosis of legionella pneumophila group 1 infections.	an antigen prepared with agar-grown legionella pneumophila group 1 killed by 0.5% phenol and suspended in 0.5% yolk sac was examined for use in the indirect immunofluorescence test for legionellosis and compared with a heat-killed antigen. the serological results of the two antigens for single and paired sera agreed well. morphological and staining characteristics were better for phenol-treated organisms. electron microscopy observation showed an apparently well-preserved cell surface. the backg ...	1984	6386881
immunochemical characterization of rough brucella lipopolysaccharides.	lipopolysaccharides (lps) were extracted from rough strains of brucella abortus and brucella melitensis and from strains of the naturally occurring rough species brucella ovis and brucella canis. brucella rough lipopolysaccharides (r-lps) were readily distinguished from brucella smooth lipopolysaccharides (s-lps) and enterobacterial r-lps, by their chemical, physical, and serological characteristics. b. ovis r-lps was differentiated from b. abortus, b. melitensis, and b. canis r-lps by its react ...	1984	6421737
evaluation of a radial immunodiffusion test for diagnosing brucellosis in sheep and its possible value for differentiating infected from brucella melitensis rev 1 vaccinated sheep.	the efficiency of 4 serological test: rose bengal (rb), complement fixation (cf), gel diffusion (gd) and radial immunodiffusion (rid) for diagnosing brucellosis in sheep was compared. rid and cf were also used to evaluate sheep serological response following rev 1 vaccination. crude smooth lipopolysaccharide (lps-s) and polysaccharide b antigens obtained from b. melitensis 16 m were used in gd and rid tests respectively. in experiment 1, two hundred and sixty five sera from adult unvaccinated sh ...	1984	6436109
brucellosis in a farming community in saudi arabia.	this communication describes a study of the prevalence of brucellosis in a farming community of saudi arabia. standard agglutination tests of sera from 21 patients were tested for brucella abortus and brucella melitensis. the results show that 8 (38%) were positive with titres of 160 or over 20 480 and with titre of complement fixation tests ranging from 8 to 64. agglutination titres of 20 to 40 were present in only 3 cases. those patients with positive agglutination titre of 160 or higher admit ...	1984	6436116
lymphocyte bacterial rosette test: methodological details and effect of metabolic inhibitors and divalent cations.	lymphocytes bind certain bacteria. this property has been utilized in the lymphocyte bacterial rosette assay to identify t and b cell subsets. here we determined the optimum conditions for the assay, studied the effect of metabolic inhibitors and divalent cations and compared bacterial rosette-forming lymphocyte subpopulations with those defined with monoclonal antibodies. the strains used were brucella melitensis, bacillus subtilis, staphylococcus aureus and escherichia coli. optimum attachment ...	1984	6440915
in vitro activity of ciprofloxacin against brucella melitensis.		1984	6489329
[practical value of examining cellular immunity in human brucellosis].		1984	6489630
[immunization of ewes and she-goats against experimental brucella melitensis infection. a comparison of rev 1 and h 38 vaccines].		1984	6489637
control of ovine brucellosis in najdi sheep in saudi arabia.	a practical programme for control of ovine brucellosis in a najdi sheep flock at haradh and protection of sheep handlers from infection was implemented. haradh sheep were serologically tested three times at six-month intervals. of 2,721 sheep subjected to the first tests, 387 (14.2%) were positive reactors. at the second test 2,072 sheep were tested and 31 (1.5%) were positive. the third test was performed on 2,963 sheep and only nine (0.3%) positive reactors were found. of eight sheep handlers ...	1984	6523581
a note on adult sheep vaccination with reduced dose of brucella melitensis rev 1.	this work describes results obtained by vaccinating pregnant ewes from a brucella melitensis-infected flock, with 5 x 10(8) cfu (half dose) rev 1. only five abortions were recorded after vaccinating 600 pregnant ewes. the serological response was followed by rose bengal, complement fixation and radial immunodiffusion tests. a short-termed, low humoral immunoresponse was produced. the radial immunodiffusion test could be more useful than the other tests for differentiating infected from vaccinate ...	1984	6529126
infection with brucella melitensis apparently acquired in the united kingdom.	a patient with brucella melitensis presented to a clinic for sexually transmitted diseases. she had unusual clinical signs including polyarthropathy and a tender enlarged spleen. her disease was apparently acquired in the united kingdom. the clinical features and immunological aspects of brucellosis are discussed.	1984	6542121
transient hemophagocytosis in brucella melitensis infection.	bone marrow phagocytic activity was studied in 40 patients with brucella melitensis infection and 35 patients with a variety of medical conditions. evidence of erythrophagocytosis was found in 14 of the 40 patients with b melitensis infection and in 15 of the 35 control cases; this was taken as baseline bone marrow phagocytic activity. considerably increased erythrophagocytosis or phagocytosis of all blood elements by increased number of histiocytes was found in one and seven patients, respectiv ...	1984	6546508
induction of autoantibodies and circulating immune complexes in mice after injection of brucella fraction "pi" or inoculation with live brucella suis.	"pi", a vaccinating fraction with b mitogenic and polyclonal activator (pca) activity extracted from brucella melitensis, was i. p. injected into b6 and db mice. in b6 mice, this led to an important but transient increase in the level of anti-ssdna antibodies, the induction of rheumatoid factor (rf) and the appearance of circulating immune complexes (cic). in db mice, on the other hand, the only significant effect consisted of a slight and ephemeral peak of cic in sera from day 7. similarly, whi ...	1983	6602586
the use of allergic test in the diagnosis of brucella melitensis infection in sheep.		1983	6626935
[stimulating action of brucella melitensis lipopolysaccharide on hemopoiesis in mice].	the bacterial mass, brucellar protective antigen and lipopolysaccharide (lps) obtained from b. melitensis stimulated the formation of endogenous colonies in the spleen of mice belonging to different strains, subjected to irradiation in sublethal doses. the maximum stimulating effect was observed when the antigens were introduced 24 hours prior to irradiation. lps introduced in the optimal dose induced an increase in the number of hemopoietic stem cells (hsc) in the s-phase of the cell cycle, thu ...	1983	6637272
serological response of sheep to brucella melitensis rev. 1 vaccine.		1983	6650024
brucellosis in riyadh, saudi arabia. a microbiological and clinical study.	during a period of two years, 30 cases of brucellosis were positively diagnosed from a total of 209 patients who reported with prolonged fever for investigation. diagnosis was made both by blood culture and serological tests. the latter included slide and tube agglutination in all cases and an enzyme linked immunosorbent assay (elisa) in 16. 11 cases (36.7%) gave negative results by the slide-agglutination screening test used at the recommended single serum dilution of 1:80. this was due to the ...	1983	6665836
brucellosis: difficulties in diagnosis and a report on 38 cases.	we have reviewed 38 patients with brucellosis who were admitted to the chaim sheba medical center between 1955 and 1977, i.e. during a 23-year period. the clinical manifestations varied greatly. diagnosis was easy when brucellosis was suspected, as in cases presenting with fever of unknown origin. in nine of the 38 cases diagnosis was difficult and delayed for several weeks since brucellosis was not suspected. some of these cases will be described in detail. the diagnosis was established in 16 p ...	1983	6668070
brucellosis in spouses and the possibility of interhuman infection.	this paper deals with a brucella infection in a laboratory worker and his wife. the diagnosis of brucellosis was proved by isolating brucella melitensis, biotype 2, from blood cultures and by the standard serum agglutination test. in the absence of any alternative epidemiologic information, the authors consider this case to be an example of interhuman infection.	1983	6668071
elevated risk of osteoarticular complications in children with acute brucella melitensis infection.	infection with brucella microorganisms is considered uncommon in the paediatric age group. we report nine paediatric patients between the ages of 8 to 17 years with acute brucella melitensis infection, who presented with spiking fever, night sweats, anorexia and malaise for 5 to 60 days prior to diagnosis. four patients developed various osteoarticular complications: migratory arthralgia, hydroarthrosis of the knees, arthritis and osteomyelitis. therapy with tetracyclines alone or in combination ...	1983	6671069
brucella melitensis: person-to-person transmission?		1983	6132121
serological response of cattle, sheep and goats in kenya vaccinated with killed brucella melitensis strain h38 adjuvant vaccine.	the antibody responses of cattle, sheep and goats to the brucella melitensis h38 adjuvant vaccine were monitored by serum (tube) agglutination, complement fixation and rose bengal plate tests. high and persisting antibody titres were induced by a single dose of this vaccine in all three species. it would be difficult to classify reactors by commonly used diagnostic tests in a control or an eradication programme using the h38 adjuvant vaccine.	1983	6346660
the use of mutants of escherichia coli for the identification of human lymphocyte subpopulations in blood smears.	in previous work we have shown that some bacteria can bind to human lymphocytes and can be used to identify lymphocyte subpopulations in conventionally stained blood smears. these bacteria are of different species or genera, which makes it difficult to study the binding mechanism. also, the main marker for b cells, brucella melitensis, is of very small size and highly pathogenic. here we show that b cells as well as some of the t cell subpopulations can be identified by different mutants obtaine ...	1983	6350450
pancytopenia due to hemophagocytosis in patients with brucellosis: a report of four cases.	in 42 patients with brucellosis who had bone-marrow cultures performed, five had hemophagocytosis, four of whom demonstrated peripheral hemocytopenia. all five patients with hemophagocytosis had brucella melitensis isolated from both blood and bone-marrow cultures. infectious diseases such as brucellosis should be considered in patients whose clinical picture suggests malignant histiocytosis, especially in geographic areas where brucellar infections are common.	1983	6833793
the presence of granulomas due to brucella melitensis in hepatitis.		1983	6833806
in vitro activity of n-formimidoyl thienamycin against 98 clinical isolates of brucella melitensis compared with those of cefoxitin, rifampin, tetracycline, and co-trimoxazole.	in vitro susceptibilities of 98 isolates of brucella melitensis to n-formimidoyl thienamycin, tetracycline, co-trimoxazole, rifampin, and cefoxitin were determined. n-formimidoyl thienamycin showed good activity which was similar to those of tetracycline and rifampin and different from that of the other beta-lactam antibiotic tested (cefoxitin), which showed poor activity. co-trimoxazole showed good activity.	1982	6980624
[enteral yersiniosis--a serious disease? current knowledge of clinical aspects, diagnosis and therapy].	enteral yersiniosis is caused either by y. enterocolitica 0-group i (syn. serotype 0:3) and 0-group v (syn. serotype 0:9) or y. pseudotuberculosis type i-vi. the clinical symptoms are mostly like enteritis, enterocolitis, acute abdomen, mesenteric lymphadenitis, or ileitis terminalis. post-infection reactions are possible like septicemia, arthritis and erythema nodosum. only cultural and serological examinations confirm the diagnosis of enteral yersiniosis. in the judgement of serological result ...	1982	7095694
the immune response of goats vaccinated with low and high doses of brucella melitensis rev 1.	the serological response, lymphocyte reactivity, and dermal hypersensitivity reactions of goats vaccinated with high and low doses of brucella melitensis rev 1 organisms to brucella antigens were studied. antibodies to soluble antigen a2 were detectable by immunoelectrophoresis (ie), appeared later than agglutinating antibodies but disappeared faster in most vaccinated animals. anti-a2 antibodies appeared earlier in goats which received the high dose. antibodies to polysaccharide b antigen were ...	1982	7112914
[development of brucella abortus, brucella melitensis and brucella and brucella suis infection infection in mice to a chronic stage and cure].		1982	7168538
efficacy of brucella melitensis rev. 1 vaccine against brucella ovis infection in rams.	the efficacy of brucella melitensis strain rev. 1 vaccine against a brucella ovis experimental infection was judged on 30 rams, 15 of which had received 8.5 x 10(8) rev. 1 at the age of 4 months. all were challenged 9 months later by conjunctival route with 5 x 10(8) b. ovis. the 30 rams were killed three months after challenge and were autopsied. genital and other organs and lymph-nodes were searched for b. ovis. only a few organs and lymph-nodes of three vaccinated rams shed b. ovis, contrary ...	1982	7168540
frequency of brucella melitensis in southern iran.		1982	7183780
comparison between subcutaneous and conjunctival route of vaccination with rev. 1 strain against brucella melitensis infection in ewes.	in areas where the prevalence of brucella melitensis infection in sheep is high, control of the disease cannot be led without the help of vaccination. the use of brucella melitensis strain rev. 1 vaccine affords a very good protection. the drawback is that this vaccine, giving rise to long-lasting antibody titers, may disturb serological screening when a return is made to a test and slaughter policy, after several years of vaccination. there is a great need for another mode of vaccination which ...	1982	7185320
[brucella melitensis endocarditis. clinical and immunological features (author's transl)].	five cases of brucella melitensis endocarditis are reported. common features included subacute course, enhanced humoral immunity, and deficient cellular immunity. appropriate antibiotic therapy led to recovery in all cases. in two patients, valvular replacement was necessary.	1982	6277006
binding of bacteria from the genus brucella to human b lymphocytes.	in previous studies, we have shown that various lymphocyte subpopulations bind different strains of bacteria of different genera and species. among these bacteria was a strain of brucella melitensis which bound to all human b lymphocytes. to determine whether the binding of b. melitensis to human b lymphocytes was strain, species, or genus characteristic, we tested the binding of b. melitensis, brucella abortus, brucella ovis, brucella suis, brucella canis and brucella neotomae to human normal a ...	1981	6783546
rapid identification of human b-lymphocytes and monocytes with rhodamine-labeled brucella melitensis.	the bacterium brucella melitensis has been shown to bind selectively to human b-lymphocytes. the specificity of this binding can be exploited in a rapid technique for the determination of human b-lymphocytes and monocytes by use of rhodamine-labeled brucella melitensis. this fluorescent labeled regent is simple to use and provides highly specific identification of human b-lymphocytes and monocytes, as demonstrated in a series of experiments characterizing known cellular markers on t- and b-lymph ...	1981	6972976
the use of salmonella schottmulleri for mapping and separation of human lymphocyte subpopulations.	human lymphocyte subpopulations (b cells, b1, b2, t1, t2, t3, and t4 cells; our denomination) have been previously identified and isolated by bacterial adherence and functional differences between them have been demonstrated. here we examined the binding properties of salmonella schottmulleri to human lymphocytes in peripheral blood smears and found that it binds to more lymphocyte subpopulations, namely b, t1, t2 and t3 cells, than any bacteria previously tested. thus, using only four bacteria: ...	1981	6976306
[isolation of brucella melitensis from pleural fluid].		1981	7253750
causes of abortion in khorassan province--mashad.	the aim of this paper is to analyse the cause of abortion and postnatal deaths in sheep on the basis of laboratory examinations. for serological examination against brucellosis and salmonellosis 899 blood samples were collected. from all tested sera 411 (45.7%) were positive to brucella melitensis antigen and only 10 (1.11%) to salmonella abortus ovis antigens. bacteriological examination were done with 50 lambs, 143 aborted foetuses and 46 vaginal swabs. brucella melitensis was stated in 7.6% i ...	1981	7301632