Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| epileptogenic effect of mianserin. | 1999 | 91069 | |
| use of permanent hair dyes and cancer in registered nurses. | 1999 | 89538 | |
| inosine analogs as substrates for adenosine kinase--influence of ionization of the n-1 proton on the rate of phosphorylation. | 1999 | 23125 | |
| successful treatment of hepatic venoocclusive disease in a bone marrow transplant patient with orthotopic liver transplantation. | 1999 | 2326879 | |
| [effect of bromocriptine on the "prolactin-like cell" of nectophrynoides occidentalis (amphibia, viviparous anura): optical and electronic microscopical studies]. | bromocriptin has an effect on prolactin-like cells of nectophrynoïdes occidentalis. 16 days after delivery, these cells are fully active in normal animals. retention of secretory granules and myelinic formations have been observed in the prolactin-like cells of females treated by cb 154. | 1999 | 97001 |
| [effect on macrophages of acute stress induced in mice by local irradiation]. | in examining the role of radiation in inducing immunosuppression in tumor-bearing mice it has been shown that the experimental acute stress inadvertently generated by the procedure is profoundly immunosuppressive. it would appear that monocytes under the influence of glucocorticoïds are sequestered in the spleen. this monocyte immobilisation leads to rapid suppression of local inflammatory reactions including delayed hypersensitivity. the increased number of marcrophages in the spleen is co-inci ... | 1999 | 96964 |
| [identification of b-, t- and "zero" lymphocytes by staining for acid phosphatase]. | 1999 | 94125 | |
| cryocrystalglobulinemia in hairy cell leukemia. | a patient with hairy cell leukemia (leukemic reticuloendotheliosis) was noted to have a spurious leukocytosis caused by a spontaneously crystallizing cryoglobulin. the cryoprotein was identified as igg lambda. an intracytoplasmic immunoglobulin demonstrable within the hairy cell was also igg lambda. the cryoglobulin spontaneously disappeared over a four day period. a reliable automated count on the coulter model s could be obtained by prewarming the blood specimen to 37 c. | 1999 | 103614 |
| responsibility of the physician in the preservation of life. | 1999 | 88925 | |
| [the myocardiopathies of hereditary neuro-muscular diseases]. | this joint work has studied the cardiomyopathies occurring in hereditary neuro-muscular disorders (270 cases). the duchenne type of disorder (74 cases) was responsible for asystole (4 cases), for cardiomegaly, and especially for abnormalities of the ecg (59 cases)--q waves and large r waves in v1 and v6. the cardiomyopathy was of the hypokinetic type, with histological evidence of degeneration of the myocardial fibres. dystrophia myotonica of steinart (23 cases) caused conductive disorders (17 c ... | 1999 | 96758 |
| pancreatic islet cytology of the himalayan toad, bufo himalayanus. | 1999 | 94018 | |
| characterization of the in vitro reassembly of tubulin derived from stable strongylocentrotus purpuratus outer doublet microtubules. | 1999 | 33707 | |
| cryotherapy for palliation in rectal carcinoma. | 1999 | 91566 | |
| studies on phosphate-solubilizing bacteria in soil and rhizosphere of different plants. ii. selection of the most efficient phosphate-dissolvers and their morphological grouping. | two hundred colonies which showed positive reaction on the plates prepared for the phosphate-dissolving bacteria from control soil rhizosphere soils and rhizoplane samples of maize, peas, or cotton were isolated at random. fifty isolates were selected as the most efficient isolates according to their capability for increasing the amounts of available phosphorus in the media with corresponding decreases in ph values. the percentage of the most efficient isolates differed according to type of plan ... | 1999 | 22210 |
| effects of x-rays on the chloroplasts of acetabularia mediterranea [proceedings]. | 1999 | 93929 | |
| study of spectrin phosphorylation and polymerization in normal and spherocytic red blood-cells [proceedings]. | 1999 | 93915 | |
| phenylketonuria variants. | 1999 | 85126 | |
| grandmother's poisoned well: report of a case of methemoglobinemia in an infant in oklahoma. | 1999 | 4688467 | |
| neonatal back-transport. cost-effectiveness. | this study examines the cost-effectiveness of returning previously ill neonates to community hospitals after treatment in a tertiary center, a concept known as "back-transport." the authors compared the charges for medical care during convalescence of a group of back-transported infants (bt infants; n = 20) with a similar group of infants who remained in a tertiary center for convalescence (nt infants; n = 20). the total charges for convalescent care (inpatient plus transport charges) for 20 rep ... | 1999 | 3918219 |
| systematic multi-modal treatment of osteosarcoma, with special reference to the role of fast neutron radiotherapy. | sixty-one patients with osteosarcoma were treated. twenty-four of these patients were managed with our systematic multi-modal treatment. overall survival rate was markedly improved chiefly by intensive systemic chemotherapy with multiple drugs, especially adriamycin and high dose methotrexate. preoperative regional intra-arterial infusion of anti-tumor drugs and fast neutron radiotherapy were employed and it was suggested that fast neutron had a higher relative biological effectiveness and a gre ... | 1999 | 115950 |
| pattern reversal evoked cortical responses in normals. a study of different methods of stimulation and potential reproducibility. | using a commercially available television set for stimulation of 78 healthy subjects, the upper limit of normal for the latency of the major positive wave (p 100) of checkerboard pattern reversal evoked potentials is practically the same as that obtained by employing slide projector combined with a rotating mirror. potentials evoked by a small pattern, for purely foveal stimulation, were often difficult to evaluate exactly. fixation of a large pattern at the upper border of the stimulus field br ... | 1999 | 93635 |
| raised level of cholesterol esters in the brain of protein undernourished mice. | 1999 | 104008 | |
| ultrasonographic demonstration of glaucomatous optic nerve excavation. | 1999 | 103912 | |
| [therapy of class ii, division 1 malocclusion without extraction of premolars during the mixed dentition period]. | 1999 | 96393 | |
| spontaneous pneumothorax in labor: case report. | 1999 | 96378 | |
| the use of visual feedback to control vocal intensity and nasalization. | a device which provides visual feedback of vocal intensity, nasalization, or a ratio of nasalization to vocal intensity, is described. the device was used in several experiments in which control of vocal intensity was a necessary component. normal and hypernasal speakers were able to control their vocal intensities successfully when using the visual display. the display also was used in an experiment designed to determine how well normal speakers can manipulate their nasalization without visual ... | 1999 | 89126 |
| analysis of the yolk proteins in drosophila melanogaster. translation in a cell free system and peptide analysis. | 1999 | 103753 | |
| [palliative chemotherapy with 5 fu and cpm in cancer of the prostate with bone metastases resistant to oestrogens. a clinical trial (author's transl)]. | the efficacy of an association of cyclophosphamide (cpm) and 5-fluorouracil was studied in 15 patients with prostate cancer not responding to oestrogen therapy, and more particularly its effect on pain due to bone metastases. no objective improvement was noted with this association, but there was a definitite reduction in bone metastases pain in 5 of the patients, with an average remission time of 4 months. half of the patients had nausea and vomiting, but in spite of this digestive intolerance ... | 1999 | 94108 |
| monoclonal antibodies against defined determinants of acetylcholine receptor. | 1999 | 91535 | |
| [evaluation of the restoration of micturition after transvesical adenomectomy of the prostate using a urodynamic flow measurement method]. | 1999 | 93823 | |
| the mesencephalic exterolateral posterior nucleus of the mormyrid fish bryenomyrus niger: efferent connections studied by the hrp method. | 1999 | 91414 | |
| haemodilution and blood-flow. | 1999 | 88643 | |
| modulatory effects of oestrogen on immunological responsiveness. ii. suppression of tumour-associated immunity in patients with prostatic cancer. | the effect of oestrogen (diethylstilboesterol diphosphate, des-p) on immunity to tumour-associated antigens in patients with prostatic cancer was evaluated by leucocyte adherence inhibition, a suggested in vitro correlate of cellular immunity. significant (p smaller than 0.05) suppression of immunity to malignant prostate was observed in thirty out of thirty-one patients following pre-incubation of their leucocytes with therapeutically significant levels of des-p. suppression of tumour-associate ... | 1999 | 93529 |
| applied teaching concepts of animated motion slides in otolaryngology. | motion is an essential part of otolaryngologic function, and an understanding of concepts of motion is critical in teaching otolaryngology. standard movie projection devices have intrinsic defects, such as considerable expense to make, complexity of operation, and a lack of flexibility. slide projection transparencies (2x2) offer instructional flexibility but could not be used to project motion until recently. using applications of gelatin films of images with polarizing light, we have been able ... | 1999 | 91151 |
| diffuse cutaneous mastocytosis: a report of neonatal onset. | a child developed diffuse cutaneous mastocytosis when 20 days old. his prognosis appears good, compared with other reports of similar neonatal onset. | 1999 | 94218 |
| [renewal of noradrenaline in different zones of the central nervous system of inbred mice strains and their recombinants]. | a good correlation exists between the learning capacity and norepinephrine metabolism in the neocortex of c57 and balb inbred mice strains, as well as their f1 hybrids and seven recombinant inbred strains derived from their cross. the animals with a better learning performance are characterised by low levels of norepinephrine, as well as a slow metabolic rate of this neurotransmitter in the cortex. such a correlation has not been found to exist in the other cerebral regions studied. | 1999 | 96976 |
| group systems of serum proteins and erythrocyte enzymes in malignant lymphoma. | four group systems of serum proteins (hp, gc, gm, km) and five group systems of erythrocyte enzymes (ap, pgm1, gpt, ak, esd) were determined in 63 patients with malignant lymphoma. statistical analysis of the distribution of the above mentioned systems in patients and polish population samples did not reveal any significant differences, which points to the lack of any correlation between the disease and the group systems under examination. | 1999 | 85572 |
| fine-needle aspiration cytology. | 1999 | 89365 | |
| development of an endosseous dental implant (iii). | 1999 | 96476 | |
| erythemal and pigmentary phototest reactions in polymorphic light eruptions. | the erythemal and pigmentary skin reactions in 110 patients with chronic polymorphic light eruptions (pmle) and in 154 control persons were investigated by phototesting with a medium pressure mercury lamp. recording the erythemal threshold (med) at 2-h intervals for up to 24 h after irradition disclosed no differences between pmle patients and controls. there was, however, a tendency for the erythemal reaction to subside more slowly in the pmle patients; this tendency reached a statistically ver ... | 1999 | 94208 |
| chemotherapy of hodgkin's disease. | during the past 10 to 15 years, there have been dramatic improvements in the prognosis of patients with advanced hodgkin's disease resulting from the development of combination chemotherapy. the long-term follow-up of patients with advanced disease treated with mopp indicates that 66 per cent of those achieving complete remissions have not relapsed and remain off therapy at five and 10 years follow-up. attempts to make additions, subtractions or modifications in the mopp regimen have not general ... | 1999 | 91466 |
| trh test in routine diagnosis and postoperative control of patients with primary thyroid diseases. | 1999 | 96643 | |
| ct of the esophagus: i. normal appearance. | the thoracic esophagus is easily demonstrable throughout its entire length on thoracic computed tomography (ct). the soft tissue planes separating the esophagus from its adjacent mediastinal structures are normally distinct. blurring or distortion of these tissue interfaces is a reliable indicator of disease. the normal ct anatomy of the esophagus and mediastinal relationships are described. air in the esophagus is considered a normal finding. | 1999 | 116493 |
| [choroidal neovascularization in the photocoagulated retina with diabetic retinopahy--a light and electron microscopic study (author's transl)]. | 1999 | 93415 | |
| [improvement in the prognosis of rapid progressive glomerulonephritis by plasmapheresis treatment]. | 1999 | 2860543 | |
| palliative embolization of arterial renal tumour supply. results in 10 cases. | palliative occlusion of the arterial renal tumour supply was performed in 10 patients and the follow up is reported. nine of the patients had no subsequent nephrectomy. spongostan (99% gelatin) was used as the embolic material in 4 patients with the addition of steel coils in 2. bucrylate was used in 6 cases. six patients are alive with survival rates presently ranging from 3 to 24 months after embolization. improvement of the survival time cannot be estimated but local symptoms such as hematuri ... | 1999 | 91307 |
| [cerebrospinal fluid monoamine metabolites and cyclic nucleotides in the patients with epilepsy (author's transl)]. | 1999 | 93481 | |
| the relationship between intracellular and extracellular potassium in normal and malnourished subjects as studied in leukocytes. | leukocyte potassium has been measured in normal subjects and in malnourished children with and without edema both on admission and during recovery. in nonedematous malnourished children the potassium content was low (328 mmol/kg dry solids) and took 2-3 weeks of rehabilitation to return to normal (374 mmol/kg dry solids). leukocytes from edematous children had normal intracellular potassium values. however, 5 days on a maintenance diet reduced the intracellular potassium in the children with kwa ... | 1999 | 99717 |
| [surgical management of thoracic and thoraco-abdominal injuries]. | from 1970 to 1974 407 patients with thoracic injuries have been treated by the authors in the 2nd surgical and thoracic surgical department of the "jános" hospital, budapest--out of them 190 patients were classified as severely injured. the material and the operative technique in isolated thoracal and combined thoracal-abdominal injuries are discussed. the importance of the operative stabilization of the thoracic wall is emphasized and fixation of the thoracic wall by means of pericostal sutures ... | 1999 | 17790 |
| captopril in a hyponatraemic hypertensive: need for caution in initiating therapy. | 1999 | 85145 | |
| determination of the quaternary structure of alpha-d-mannosidase from medicago sativa l. [proceedings]. | 1999 | 93904 | |
| bacterial infections of the small intestine and colon. | enterotoxigenic escherichia coli, vibrio cholerae o1, campylobacter jejuni, salmonella species, and shigella species are major causes of morbidity and death in diarrheal disease. more recently recognized pathogens are v. cholerae o139 and enterohemorrhagic e. coli. in addition to this, several presumptive virulence factors have been identified in diarrheagenic e. coli and in other species. to confirm these as virulence factors we need good diagnostic tools and good epidemiological studies. these ... | 1999 | 17023917 |
| [obtention of monoclonal antibodies against human secretory iga]. | in order to determine the iga levels in secretions, the authors engaged themselves to obtain monoclonal antibodies against this immunoglobulin and to show their possible utilization in the development of quantitation methods. 6 monoclonal antibodies were obtained: one against the secretory component (sc) and five against the heavy-chain (hc) of iga. iga purified from human colostrum was used as immunogen (hc). the immunoglobulin isotypes (igg2a and igg1) were determined in 2 monoclonal antibodie ... | 1999 | 10887553 |
| [standardization and evaluation of the modified vibriocidal assay]. | the vibriocidal antibody test is a reliable and well-documented method to determine bacterial antibodies to vibrio cholerae 01 antigens. it consists of mixing serum dilutions and a steady quantity of bacteria and supplement to cause cell lysis. titer is determined by visual observation. in this paper, we implemented a change in the presented method where a ph and glucose indicator was added to the culture medium used to stop the reaction, which allowed a quicker reading by any person who are not ... | 1999 | 10887580 |
| investigation of the stability of thiosialosides toward hydrolysis by sialidases using nmr spectroscopy. | [formula: see text] 1h nmr spectroscopy has been used to investigate whether the alpha(2-->6)-linked thiosialoside 3 and the alpha(2-->3)-linked thiosialoside 9 are hydrolyzed in the presence of vibrio cholerae sialidase. similarly, the hydrolysis of the o-ketosides neu5ac-2-o-alpha-(2-->3)-gal beta me (4) and the alpha-(2-->6)-sialyllactoside 7, representing natural alpha(2-->3)- and alpha(2-->6)-linked sialosides, respectively, was investigated. the results of the 1h nmr experiments clearly de ... | 1999 | 10822584 |
| [the capacity to lyse human erythrocytes as a factor in the pathogenicity of vibrio cholerae]. | 1999 | 10876862 | |
| [the determination of ribulose diphosphate carboxylase activity in inactive variants of vibrio cholerae eltor]. | 1999 | 10876865 | |
| [sensitivity to cotrimoxazole of bacteria isolated at the central university hospital of fann, dakar]. | this study concern a survey of bacterial resistance to cotrimoxazole; 510 strains of enterobacteria (167), vibrio cholerae(206) and staphylococcus aureus(137) were tested by disc diffusion and agar dilution methods. an interview was conducted with 86 health personals to appreciate the influence of prescription. staphylococcus aureus were the most susceptible bacteria (13% of resistance), and vibrio cholerae the most resistant (95%). related to the gender, enterobacteria present 43 to 72% of resi ... | 1999 | 10797980 |
| [monitoring of life-threatening infection pathogens in relation to the problem of prediction of critical situations]. | the epidemic situation in the context of many infectious diseases caused by bacteria is presently assessed as being poor in russia and other countries. the spectrum of the pathogens that can deteriorate epidemic well-being is highly wide. the epidemic situation in terms of many infectious diseases, including those caused by such causative agents as bacillus anthracis, vibrio cholerae, yersinia pestis, francisella tularensis and others may deteriorate due to the emergence of their modified forms ... | 1999 | 10709458 |
| inorganic polyphosphate: a molecule of many functions. | inorganic polyphosphate (poly p) is a chain of tens or many hundreds of phosphate (pi) residues linked by high-energy phosphoanhydride bonds. despite inorganic polyphosphate's ubiquity--found in every cell in nature and likely conserved from prebiotic times--this polymer has been given scant attention. among the reasons for this neglect of poly p have been the lack of sensitive, definitive, and facile analytical methods to assess its concentration in biological sources and the consequent lack of ... | 1999 | 10872445 |
| [the comparative characteristics of the pathogenic action of vibrio cholerae of the non-o1 group o139 serovar and of the o1 group on the intestinal apud system of suckling rabbits]. | the comparative study of the enteropathogenic action of v. cholerae strains of group non-o1, serovar o139, and group o1 with different virulence on the apud system of the intestine of suckling rabbits after intraenteral infection revealed that v. cholerae of group non-o1 induced inflammatory changes in the intestine and the pronounced toxic lesion of parenchymal organs. this was accompanied by a decrease in the number of apudocytes and an increase in the functional tension of the apud system. af ... | 1999 | 10851981 |
| [the hemo-cytolysin of vibrio cholerae: its mechanisms of action in vitro and in vivo]. | 1999 | 10852004 | |
| [the current status of the problem of vibrio cholerae variability]. | 1999 | 10852073 | |
| an el tor cholera outbreak in maldah district, west bengal. | an outbreak of cholera occurred in maldah district, west bengal during july-august 1998. attack rate was 34/1000. cases were more (59.3%) amongst adults (> 15 years.). v. cholerae 01 biotype e1 tor serotype ogawa was isolated as a single pathogen from 52.9% (9/17 samples examined). all v. cholerae strains belonged to phage type 2 (basu and mukherjee scheme) and type 27 (new phage type scheme). the strains were resistant to co-trimoxazole, furazolidone, ampicillin, streptomycin and nalidixic acid ... | 1999 | 10810587 |
| some emerging food and water borne pathogens. | emerging pathogens are those infective organisms whose incidence has recently increased or is likely to increase during the next two decades due to changes in demography, food habits, food technology, commerce, water sources and environmental factors. some important emerging food and water borne bacterial pathogens include listeria monocytogenes, campylobacter jejuni, yersinia enterocolitica, salmonella enteritidis, escherichia coli o157: h7, vibrio cholerae biotype e1 tor serotype 0139, vibrio ... | 1999 | 10810592 |
| the seasonal variation in the microbial agents implicated in the etiology of diarrheal diseases among children in lao people's democratic republic. | during 1994-1995, the etiological structure of children's diarrhea was monitored in outpatients at mahosot hospital, vientiane, lao pdr. of the 191 children studied, 42% had stool specimens positive for enteropathogens; 22% had rotavirus, 21.5% enteropathogenic e. coli, 4.7% shigella flexneri, 2.9% campylobacter jejuni, 2.1% shigella sonnei, and 0.5% each of giardia intestinalis and entamoeba histolytica. no vibrio cholerae and salmonella spp. was detected in this monitoring. in children under f ... | 1999 | 10774703 |
| incidence of vibrio cholerae in different age groups and sex in aurangabad province isolated during january 1994 to december 1994. | in our study age group 1-10 years was mostly affected as also in 1992 and 1993 because of low health consciousness and poor personal hygiene at this age. | 1999 | 10776515 |
| [in vitro activities of levofloxacin and other antibiotics against fresh clinical isolates]. | in this study, the in vitro activity of levofloxacin (lvfx) against 1,020 fresh bacterial clinical isolates was compared with the activities of a range of ofloxacin, ciprofloxacin (cpfx), ampicillin (abpc), cefaclor, cefpodoxime, methicillin and benzylpenicillin. the clinical isolates except vibrio cholerae were collected in japan during 1998 from patients with infectious diseases. mics were determined using the agar dilution method according to the recommendations by the japan society of chemot ... | 1999 | 10746192 |
| the vellore vibrio watch 1996-98. | 1999 | 10717740 | |
| [isolation of vibrio cholerae non 01 from patients with acute gastroenteritis]. | it was assay by biochemical and immunological tests, strains of v. cholerae non-01 non-0139 isolated from two patients of the province of córdoba, argentina. they showed episodes of acute gastroenteritis. strains non-01 non-0139 were isolated from water samples ingested by patients. we conclude that strains identified from patients would have the source from contaminated environmental water by v. cholerae. | 1999 | 10668268 |
| super-integrons. | integrons represent the primary mechanism for antibiotic resistance gene capture and dissemination among gram-negative bacteria. the recent finding of super-integron (si) structures in the genomes of several bacterial species has expanded their role in genome evolution. the vibrio cholerae superintegron is gathered in a single chromosomal super-structure harbouring hundreds of gene cassettes. the encoded functions, when identifiable, are linked to adaptations extending beyond antibiotic resistan ... | 1999 | 10673003 |
| safety, immunogenicity, and lot stability of the whole cell/recombinant b subunit (wc/rctb) cholera vaccine in peruvian adults and children. | to assess the safety, immunogenicity, and lot stability of the whole cell/recombinant b subunit cholera vaccine, 2 lots manufactured in june 1991 and february 1992 were tested in january 1995. two oral doses of vaccine or placebo given 2 weeks apart were given with buffer to 216 peruvian adults and children. symptoms were elicited for 3 days after each dose. serum and plasma specimens obtained from each volunteer before vaccination and 10-14 days after the second dose were tested for vibriocidal ... | 1999 | 10674661 |
| application of pcr for detection of toxigenic vibrio cholerae o1 in water samples during an outbreak of cholera. | 1999 | 10696413 | |
| [cholera in guinea: the 1994-1995 epidemic]. | since the first outbreak in 1970, cholera epidemics have occurred regularly in guinea. until 1994, epidemics recurred every 8 years and were confined to the capital and coastal areas. the first cases in every epidemic were observed in coastal lagoons near the sierra leone border. in 1994, the disease demonstrated its migratory ability as the first cases were reported in towns located in far eastern inland areas. spread of the disease from war-torn sierra leone and liberia where epidemics have al ... | 1999 | 10701212 |
| cytotoxicity of non o1, non o139 vibrios isolated from fresh water bodies in vellore, south india. | the samples of plankton, soil sediment and water from a pond, a lake and a moat respectively in and around vellore were studied for environmental vibrios. vibrios were isolated from all these specimens after enrichment in alkaline peptone water and subculture on selective media. non o1, non o139 vibrio cholerae, aeromonas spp. and plesiomonas spp. were isolated. there were no isolates of v. cholerae serogroup o1 and o139. representative strains of non o1 and non o139 v. cholerae from environment ... | 1999 | 10680299 |
| cholera vaccine inactivated oral. | 1999 | 10641223 | |
| outbreak of cholera in arid zone of bikaner. | bikaner being an arid zone was more or less unaffected by cholera until 1994 when an outbreak of clinical cholera occurred. we isolated 64 vibrio cholerae strains out of 475 stool samples received (isolation rate 13.47%). all the vibrio strains belonged to biotype el tor serotype ogawa. low isolation rate was probably related to the poor transportation and medical facilities available at remote areas and indiscriminate and irrational use of antibiotics. the antimicrobial susceptibility testing o ... | 1999 | 10645099 |
| emergence of nalidixic acid resistant vibrio cholerae o-1 in karachi. | 1999 | 10647244 | |
| the synthesis and evaluation of novel sialic acid analogues bound to matrices for the purification of sialic acid-recognising proteins. | a novel n-acetylneuraminic acid analogue, 2-s-(5'-aminopentyl) 5-acetamido-3,5-dideoxy-2-thio-d-glycero-alpha-d-galacto-2- nonulopyranosidonic acid, as well as the thiosialoside 2-s-(2'-aminoethyl) 5-acetamido-3,5-dideoxy-2-thio-d-glycero-alpha-d-galacto-2- nonulopyranosidonic acid, have been synthesised and successfully coupled to cnbr-activated sepharose 4b through the terminal amino group. the resultant affinity resins have proved efficient in purifying a number of sialic acid-recognising pro ... | 1999 | 10637984 |
| [genetic control of vibrio cholerae pathogenicity: the temperate filamentous phage ctx, coding for cholera toxin and the "island of pathogenicity"]. | reviews modern data on the genetic control of the key factors of vibrio cholerae pathogenicity: cholera toxin and toxin-coregulated adhesion pili. pays special attention to the temperate filamentous ctx bacteriophage, whose genome contains structural genes of cholera toxin, and the "pathogenicity island" carrying tcp genes responsible for the most important factor of the human small intestine colonization with v. cholerae. discusses the mechanism of coordinated regulation of the activity of the ... | 1999 | 10621931 |
| studies on vaccines against cholera. synthesis of neoglycoconjugates from the hexasaccharide determinant of vibrio cholerae o:1, serotype ogawa, by single-point attachment or by attachment of the hapten in the form of clusters. | the terminal hexasaccharide of the o-antigen of vibrio cholerae o:1, serotype ogawa, has been synthesized in the form of a glycoside whose aglycon (linker) allows conjugation to carrier proteins by reductive amination. the conjugate obtained from direct, single-point attachment of the linker-equipped hapten to chicken serum albumin (csa) contained seven hapten residues/csa. a neoglycoconjugate containing the carbohydrate antigen in the form of clusters was obtained using, as a hapten subcarrier, ... | 1999 | 10614066 |
| a multifunctional atp-binding cassette transporter system from vibrio cholerae transports vibriobactin and enterobactin. | vibrio cholerae uses the catechol siderophore vibriobactin for iron transport under iron-limiting conditions. we have identified genes for vibriobactin transport and mapped them within the vibriobactin biosynthetic gene cluster. within this genetic region we have identified four genes, viup, viud, viug and viuc, whose protein products have homology to the periplasmic binding protein, the two integral cytoplasmic membrane proteins, and the atpase component, respectively, of other iron transport s ... | 1999 | 10601218 |
| molecular approaches for safer and stronger vaccines. | progress in molecular biology and biotechnology is making possible the development of new vaccines or the improvement of already existing ones. recombinant dna technology, genetic attenuation of bacterial and viral pathogens and their use as vectors for heterologous proteins, expression of microbial antigens in transgenic edible plants, and naked nucleic acid technology represent the most popular approaches hitherto adopted. a successful biotechnological approach to the development of new and im ... | 1999 | 10603647 |
| aeromonas-associated diarrhoea in bangladeshi children: clinical and epidemiological characteristics. | we studied the clinical and epidemiological features associated with aeromonas diarrhoea by a hospital survey of 7,398 children under 5 years of age presenting with diarrhoea. the data were actually based upon two cohorts from this survey, the majority of the data being identified from 405 (5.5%) in whom aeromonas was the sole enteric pathogen. aeromonas caviae was the most prevalent species, accounting for 32% (129/405) of all isolates. eighty-three per cent of children with aeromonas-associate ... | 1999 | 10605516 |
| n-terminus of mature heat-labile enterotoxin chain b is critical for its extracellular secretion in vibrio cholerae. | the effects of addition of a few amino acids to the amino- and carboxy-terminal regions of the mature portion of the heat-labile enterotoxin chain b (ltb) of escherichia coli on protein export, secretion and assembly were investigated. in e. coli, ltb (secretory protein) with or without the extension at the n- or c-terminus accumulated in the periplasmic fraction. for vibrio cholerae, ltb with the extension at the c-terminus was exported to the periplasm followed by secretion to the extracellula ... | 1999 | 10606749 |
| solution conformation and dynamics of the trisaccharide fragments of the o-antigen of vibrio cholerae o1, serotypes inaba and ogawa. | the conformational behavior of the trisaccharide fragments of the ogawa and inaba vibrio cholera serotypes has been studied using nmr and molecular dynamics (md). the obtained results indicate that there are no significant differences in the major conformation and in the extent of motion of the glycosidic torsions of these molecules. the differences in biological activity are probably not due to conformational effects but to van der waals and/or hydrogen bonding interactions between the antigens ... | 1999 | 10612004 |
| regulation and temporal expression patterns of vibrio cholerae virulence genes during infection. | the temporal expression patterns of the critical vibrio cholerae virulence genes, tcpa and ctxa, were determined during infection using a recombinase reporter. tcpa was induced biphasically in two temporally and spatially separable events in the small intestine, whereas ctxa was induced monophasically only after, and remarkably, dependent upon, tcpa expression; however, this dependence was not observed during in vitro growth. the requirements of the virulence regulators, toxr, tcpp, and toxt, fo ... | 1999 | 10612398 |
| direct interaction of the epsl and epsm proteins of the general secretion apparatus in vibrio cholerae. | the general secretion pathway of gram-negative bacteria is responsible for extracellular secretion of a number of different proteins, including proteases and toxins. this pathway supports secretion of proteins across the cell envelope in two distinct steps, in which the second step, involving translocation through the outer membrane, is assisted by at least 13 different gene products. two of these components, the cytoplasmic membrane proteins epsl and epsm of vibrio cholerae, have been purified ... | 1999 | 10322014 |
| uptake and retention of vibrio cholerae non-o1, salmonella typhi, escherichia coli and vibrio harvey by mussels in seawater. | vibrio cholerae non o1 is known to persist in estuarine and freshwater environments. experiments evaluated the amount of microorganisms accumulated in mussels maintained in static seawater, contaminated with 10(4) to 10(6) cells/ml and the depuration time required in circulating water. accumulation and retention times were compared with those for escherichia coli, salmonella typhi and vibrio harvey. e. coli and s. typhi accumulated to a greater extent and were released from mussels more quickly ... | 1999 | 10322612 |
| on the structural stability of a small bioactive peptide of potential use in biotechnology. | a tridecapeptide with the sequence cceiccnpacfgc has been synthesized to reproduce the active moiety of a heat stable enterotoxin from vibrio cholerae. the proton nmr analysis indicates, for the active synthetic fragment, a rigid secondary structure stabilised by three disulfide bridges. such a rigid peptide, suitably detoxified and activated, could be a good candidate to be used as a carrier for linear bioactive peptides or other functional groups. | 1999 | 10333175 |
| disappearance of glyceraldehyde 3-phosphate dehydrogenase from erythrocyte membrane by hemolysis with thermostable direct hemolysin of vibrio parahaemolyticus or vibrio cholerae el tor hemolysin. | it is believed that the thermostable direct hemolysin (tdh) of vibrio parahaemolyticus and el tor hemolysin (eth) of vibrio cholerae damage erythrocytes and other cells by acting as pore-forming toxins. in this study, we found that a protein band with a molecular weight of 37,000 daltons specifically disappeared from erythrocyte membrane after hemolysis by tdh and eth, but not after hypotonic hemolysis. the 37 kda band was identified as glyceraldehyde 3-phosphate dehydrogenase (g3pd), a glycolyt ... | 1999 | 10338202 |
| antibody responses in the lower respiratory tract and male urogenital tract in humans after nasal and oral vaccination with cholera toxin b subunit. | nasal vaccine delivery is superior to oral delivery in inducing specific immunoglobulin a (iga) and igg antibody responses in the upper respiratory tract. although an antibody response in the nasal passages is important in protecting against primary colonization with lung pathogens, antibodies in the lungs are usually required as well. we immunized 15 male volunteers twice nasally or orally with cholera toxin b subunit (ctb) and determined the specific antibody levels in serum, bronchoalveolar l ... | 1999 | 10338495 |
| characterization of the antiseptic-resistance gene qace delta 1 isolated from clinical and environmental isolates of vibrio parahaemolyticus and vibrio cholerae non-o1. | the nucleotide sequence and mechanism of action were examined on the antiseptic-resistance gene qace delta 1 that had been isolated from pseudomonas aeruginosa, vibrio parahaemolyticus and vibrio cholerae non-o1. the nucleotide sequences of qace delta 1 genes isolated from environmental isolates of v. cholerae non-o1 and v. parahaemolyticus differed by one base from that of the gene from p. aeruginosa. escherichia coli c600 that harbored qace delta 1 genes from several strains of vibrio spp. exh ... | 1999 | 10339831 |
| isolation of vibrio cholerae from diseased farm animals and surface water in the netherlands. | 1999 | 10343379 | |
| occurrence, diversity, and pathogenicity of halophilic vibrio spp. and non-o1 vibrio cholerae from estuarine waters along the italian adriatic coast. | the occurrence, diversity, and pathogenicity of vibrio spp. were investigated in two estuaries along the italian adriatic coast. vibrio alginolyticus was the predominant species, followed by vibrio parahaemolyticus, non-o1 vibrio cholerae, and vibrio vulnificus. by using a biochemical fingerprinting method, all isolates were grouped into nine phenotypes with similarity levels of 75 to 97.5%. the production of toxins capable of causing cytoskeleton-dependent changes was detected in a large number ... | 1999 | 10347072 |
| response of pathogenic vibrio species to high hydrostatic pressure. | vibrio parahaemolyticus atcc 17802, vibrio vulnificus atcc 27562, vibrio cholerae o:1 atcc 14035, vibrio cholerae non-o:1 atcc 14547, vibrio hollisae atcc 33564, and vibrio mimicus atcc 33653 were treated with 200 to 300 mpa for 5 to 15 min at 25 degrees c. high hydrostatic pressure inactivated all strains of pathogenic vibrio without triggering a viable but nonculturable (vbnc) state; however, cells already existing in a vbnc state appeared to possess greater pressure resistance. | 1999 | 10347079 |
| the serodiagnosis of infections caused by verocytotoxin-producing escherichia coli. | patients with haemolytic uraemic syndrome (hus) and haemorrhagic colitis (hc) produce serum antibodies to the lipopolysaccharides (lps) of escherichia coli o157 and certain other e. coli serogroups. patients may also make salivary antibodies to the lps of e. coli o157. serological tests based on these antibodies can be used to provide evidence of infection in the absence of culturable vtec or the toxins they produce. serum antibodies to lps persist for several months following onset of disease, ... | 1999 | 10347867 |
| comparison of the heme iron utilization systems of pathogenic vibrios. | vibrio alginolyticus, vibrio fluvialis, and vibrio parahaemolyticus utilized heme and hemoglobin as iron sources and contained chromosomal dna similar to several vibrio cholerae heme iron utilization genes. a v. parahaemolyticus gene that performed the function of v. cholerae huta was isolated. a portion of the tonb1 locus of v. parahaemolyticus was sequenced and found to encode proteins similar in amino acid sequence to v. cholerae hutw, tonb1, and exbb1. a recombinant plasmid containing the v. ... | 1999 | 10348876 |
| a role for the mannose-sensitive hemagglutinin in biofilm formation by vibrio cholerae el tor. | while much has been learned regarding the genetic basis of host-pathogen interactions, less is known about the molecular basis of a pathogen's survival in the environment. biofilm formation on abiotic surfaces represents a survival strategy utilized by many microbes. here it is shown that vibrio cholerae el tor does not use the virulence-associated toxin-coregulated pilus to form biofilms on borosilicate but rather uses the mannose-sensitive hemagglutinin (msha) pilus, which plays no role in pat ... | 1999 | 10348878 |
| antibodies to the truncated (short) form of 'o' polysaccharides (tfop) of vibrio cholerae o139 lipopolysaccharides protect mice against experimental cholera induced by encapsulated o139 strains and such protection is mediated by inhibition of intestinal colonization of vibrios. | an antiserum raised against the lipopolysaccharides (lps) of an encapsulated vibrio cholerae o139 strain was shown to induce passive protection against challenge with o139, but not o1, organisms. subsequent experiments, however, revealed that the purified lps, obtained by the conventional phenol-water extraction method, contained capsular polysaccharide (cps) material. therefore, another antiserum was raised by immunization with electrophoresed gel-cut material containing only the truncated (sho ... | 1999 | 10438055 |