Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| dna sequence of the simian varicella virus (svv) gh gene and analysis of the svv and varicella zoster virus gh transcripts. | the varicella zoster virus (vzv) glycoprotein h (gh) stimulates vzv-specific immune responses and may be involved in virus penetration. this study reports the genomic map position and the dna sequence of a simian varicella virus (svv) homologue of the vzv gh gene. a 32p-labeled vzv gh-specific dna probe hybridized to the hindiii b subclone of the svv bamhi b restriction endonuclease (re) fragment. the dna sequence of the svv hindiii b subclone was determined and analysis indicated a svv open rea ... | 1995 | 8546010 |
| computer modeling of prototypic and aberrant nucleocapsids of varicella-zoster virus. | 1995 | 8553532 | |
| [cns vasculitis after ophthalmic herpes zoster infection]. | we present a case of cerebral vasculitis secondary to infection with varicella zoster virus which appeared in a 27 year old patient after a latency period of eight weeks. we emphasize the usefulness of angioresonance as a fitting means of evolutive follow up without the need to carry out control cerebral angiography. we likewise point out the usefulness of acyclovir as the sole treatment necessary in non-complicated cases. | 1995 | 8556594 |
| genome differences among varicella-zoster viruses isolated in thailand. | the dnas of 17 isolates of varicella-zoster virus (vzv) obtained from 17 thai individuals with normal varicella or zoster infections (no underlying diseases) were compared by restriction endonuclease analysis using bgli, psti, ecori, smai and bamhi. the dna of the japanese strain, kawaguchi, was also conducted as a reference dna. all of virus isolates were confirmed for existence of vzv and vzv-dna by immunofluorescent test and dna-hybridization, respectively. almost all of the thai epidemiologi ... | 1995 | 9139375 |
| recurrent brainstem encephalitis associated with herpes simplex virus type 1 dna in cerebrospinal fluid. | a 47-year-old man had recurrent signs and symptoms of brainstem encephalitis over a 4-year period. although csf viral cultures were repeatedly negative, herpes simplex virus type 1 (hsv-1) dna was detected in csf by polymerase chain reaction (pcr). hsv-1-specific antibodies were absent at the time of the first positive pcr test, but csf seroconversion to high hsv-1-specific antibody titer subsequently occurred. csf antibody to cytomegalovirus (cmv) and varicella-zoster virus (vzv) was not detect ... | 1995 | 8848201 |
| simian varicella virus antibody response in experimental infection of african green monkeys. | the humoral immune response to simian varicella virus (svv) was investigated following primary and secondary experimental infection of african green monkeys. neutralization and immunoprecipitation assays were used to determine antibody titers to svv throughout the course of infection. the immune response to specific viral polypeptides was analyzed by immunoprecipitation analysis. the results demonstrate that the simian varicella model offers a useful approach to investigate immune mechanisms in ... | 1995 | 8750501 |
| development of live attenuated varicella vaccine. | 1995 | 8608424 | |
| herpesvirus infections. | some of the more common herpesviruses, human herpesvirus types 1, 2, and 6; varicella zoster virus; epstein-barr virus; and cytomegalovirus, are discussed. discussions include the incidence, pathogenesis, clinical manifestations, histopathology, and differential diagnosis of these herpesviruses. treatment options are also reviewed. | 1995 | 11362484 |
| role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis. | the herpes simplex virus type 1 (hsv-1) ul41 gene product, virion host shutoff (vhs), has homologs among five alphaherpesviruses (hsv-1, hsv-2, pseudorabies virus, varicella-zoster virus, and equine herpesvirus 1), suggesting a role for this protein in neurotropism. a mutant virus, termed ul41nhb, which carries a nonsense linker in the ul41 open reading frame at amino acid position 238 was generated. ul41nhb and a marker-rescued virus, ul41nhb-r, were characterized in vitro and tested for their ... | 1995 | 7474089 |
| mode of action of (r)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine against herpesviruses. | the activity, metabolism, and mode of action of (r)-9-[4-hydroxy-2-(hydroxymethyl)butyl]guanine (h2g) against herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) and varicella-zoster virus (vzv) were studied. compared to acyclovir (acv), h2g has superior activity against vzv (50% inhibitory concentration of 2.3 microm) and epstein-barr virus (50% inhibitory concentration of 0.9 microm), comparable activity against hsv-1, and weaker activity against hsv-2. the antiviral effect on hsv-1 showed ... | 1995 | 7486922 |
| detecting varicella-zoster virus dna in iridocyclitis using polymerase chain reaction: a case of zoster sine herpete. | 1995 | 7487588 | |
| delayed herpes zoster pseudodendrites. polymerase chain reaction detection of viral dna and a role for antiviral therapy. | the late-onset pseudodendrites, delayed corneal mucous plaques, of herpes zoster ophthalmicus are reported to be of mechanical or immune origin and to be worsened by antiviral therapy. | 1995 | 7487598 |
| detection of latent varicella zoster virus dna and human gene sequences in human trigeminal ganglia by in situ amplification combined with in situ hybridization. | varicella zoster virus (vzv) establishes latency in sensory ganglia following primary infection (chickenpox) and may reactivate decades later to produce zoster (shingles). the presence of vzv dna in latently infected ganglia has been demonstrated by southern blot hybridization as well as by polymerase chain reaction of dna extracted from latently infected ganglia. conflicting results have been obtained by in situ hybridization studies to determine the cell type in the ganglia harboring the laten ... | 1995 | 7503701 |
| synthesis and antiproliferative and antiviral activity of 2'-deoxy-2'-fluoroarabinofuranosyl analogs of the nucleoside antibiotics toyocamycin and sangivamycin. | the glycosylation of 3,4-dicyano-2-[(ethoxymethylene)amino]pyrrole (7) with 2-deoxy-2-fluoro-alpha-d-erythro-pentofuranosyl bromide (2) furnished an anomeric mixture of nucleosides (8a,b). this mixture was separated, and the individual anomers were treated with methanolic ammonia to effect a concomitant deblocking and ring closure. this furnished both anomers of 2'-deoxy-2'-fluoro-ara-toyocamycin (9a,b). the cyano moiety of 9b was converted to the carboxamide moiety to furnish 2'-deoxy-2'-fluoro ... | 1995 | 7562946 |
| interpretation of intraocular and serum antibody levels in necrotizing retinitis. | intraocular antibodies have been measured as a diagnostic aid in necrotizing retinitis but interpretation of results may be difficult. | 1995 | 7569351 |
| varicella-zoster virus hepatitis and a suggested management plan for prevention of vzv infection in adult liver transplant recipients. | 1995 | 7570963 | |
| assays for antiviral drug resistance. | 1995 | 7574542 | |
| site-directed mutagenesis of varicella-zoster virus thymidylate synthase. analysis of two highly conserved regions of the enzyme. | we have constructed a series of mutants to study the role of two structurally and functionally important regions of thymidylate synthase (ts) from varicella-zoster virus (vzv). the first centres on a conserved glycine residue in the beta-kink of beta-strand i, a partially buried region of the protein that is important for dimer interactions and the formation of the active site. we show that the glycine residue located in beta-strand i is not essential for enzyme activity and that beta-strand i c ... | 1995 | 7607223 |
| brain stem encephalitis due to varicella-zoster virus in a patient with aids. | we describe a patient infected with human immunodeficiency virus (hiv) who had localized brain stem encephalitis due to varicella-zoster virus (vzv) and no cutaneous eruption. diagnosis of the infection was based on the presence of cowdry type a intranuclear inclusions in neurons, astrocytes, and oligodendrocytes positive for vzv (as shown by immunochemical staining). although this infection is rare, we demonstrate the need for clinicians to include vzv infection in the differential diagnosis of ... | 1995 | 7620026 |
| varicella vaccine guidelines. | 1995 | 7630706 | |
| new method for the extraction of viral rna and dna from cerebrospinal fluid for use in the polymerase chain reaction assay. | a new, rapid, and simple method for the isolation of either rna or dna from cerebrospinal fluid samples for subsequent amplification by specific polymerase chain reaction (pcr) assays is described. the technique involves a single extraction with a guanidinium thiocyanate acid (guscn) buffer, and does not require the use of organic solvents. applied to the recovery of enteroviral rna, herpes simplex virus (hsv) and varicella-zoster virus (vzv) dnas the method has proved to be of equivalent or bet ... | 1995 | 7635925 |
| differential in situ hybridization for herpes simplex virus typing in routine skin biopsies. | a herpes simplex virus (hsv) type 2 specific recombinant plasmid probe designated ph2s3 was constructed from non-hsv-1 crossreactive regions of the hsv-2 genome. dna in situ hybridization on in vitro reconstructed tissue samples of sheep collagen matrix impregnated with herpes virus-infected human cells was used to demonstrate absence of crossreactivity of the ph2s3 probe with hsv-1 and varicella zoster virus under stringent posthybridization washing conditions. it was demonstrated that dna in s ... | 1995 | 7635926 |
| construction of a herpes simplex virus/varicella-zoster virus (hsv/vzv) thymidine kinase recombinant with the pathogenic potential of hsv and a drug sensitivity profile resembling that of vzv. | a recombinant of herpes simplex virus (hsv) was constructed in which the hsv thymidine kinase (tk) gene was deleted and the varicella-zoster virus (vzv) tk gene was introduced into the us5 region under the control of the human cytomegalovirus ie promoter. infection with the recombinant (r18) led to the induction of tk although the kinetics of synthesis resembled those of a 'late' gene product. the recombinant was virulent in the zosteriform mouse model with the pattern of pathogenesis similar to ... | 1995 | 7636473 |
| tropism of varicella-zoster virus for human cd4+ and cd8+ t lymphocytes and epidermal cells in scid-hu mice. | to investigate the cell tropism and pathogenicity of varicella-zoster virus (vzv) strains, we analyzed vzv replication by using scid-hu mice that carry human fetal thymus/liver implants under the kidney capsule or as subcutaneous fetal skin implants. mrc-5 cells infected with wild-type vzv or the oka strain, used in the live attenuated varicella vaccine, were injected into the implants. the implants were surgically removed 2, 7, 14, and 21 days postinfection. the vzv titer from infected thymus/l ... | 1995 | 7636965 |
| fulminant hepatitis revealing primary varicella in a renal graft recipient. | 1995 | 7652908 | |
| [herpes zoster: a herpes non-simplex disease]. | 1995 | 7656839 | |
| impaired expression of interleukin 2 receptor and cd45ro antigen on lymphocytes from children with acute lymphoblastic leukemia in response to cytomegalovirus and varicella-zoster virus. | the expression of cd25 and cd45ro in lymphocytes from patients with acute lymphoblastic leukemia in response to cytomegalovirus and varicella-zoster virus antigens was analyzed by flow cytometry. it is suggested that the defects in the immune response to cytomegalovirus and varicella-zoster virus in patients with acute lymphoblastic leukemia on chemotherapy are in the expression of cd25 and in the switching of cd45ra cells to cd45ro cells. | 1995 | 7664187 |
| visceral varicella zoster infection after bone marrow transplantation without skin involvement and the use of pcr for diagnosis. | a 41-year-old patient with acute myeloid leukemia was transplanted from an hla-identical but abo-incompatible sibling. the post-transplant course was complicated by pure erythrocyte aplasia and mild chronic graft-versus-host disease. eleven months after transplant while on steroid therapy she developed abdominal pain rapidly followed by fatal fulminant hepatic failure. varicella zoster virus (vzv) was detected using the polymerase chain reaction from blood and liver obtained at necropsy even tho ... | 1995 | 7670413 |
| [meeting report of the 2nd international conference on varicella zoster virus, 7-8 july 1994 in paris]. | 1995 | 7672996 | |
| combined use of complement and anti-immunoglobulin in an enhanced neutralization assay for antibodies to varicella-zoster virus. | an enhanced neutralization assay was developed to permit the sensitive, specific, and reproducible measurement of antibodies to varicella-zoster virus (vzv). optimal neutralization was achieved using a combination of guinea pig complement (c') and rabbit anti-human igg. this provided 625-, 160- and 13- to 64-fold increases in dilution endpoints of human post-zoster serum, varicella-zoster immune globulin and representative sera from recipients of live attenuated varicella vaccine, respectively, ... | 1995 | 7673386 |
| vaccines released. | 1995 | 7677269 | |
| varicella-zoster virus gene 63 encodes an immediate-early protein that is abundantly expressed during latency. | varicella-zoster virus (vzv) gene 63 encodes a protein with a predicted molecular mass of 30.5 kda which has amino acid similarities with the immediate-early (ie) protein 22 (icp-22) of herpes simplex virus type 1. in order to study the expression of this protein during lytic and latent infection, gene 63 was cloned in frame and downstream from the glutathione-s-transferase gene, expressed as a fusion protein, and purified. in vzv-infected vero cells, antibodies directed against this protein det ... | 1995 | 7707559 |
| [acute retinal necrosis caused by varicella zoster virus]. | 1995 | 7711128 | |
| recommendations for the use of live attenuated varicella vaccine. american academy of pediatrics committee on infectious diseases. | 1995 | 7724330 | |
| from the food and drug administration. | 1995 | 7745758 | |
| varicella-zoster virus gene 63: transcript mapping and regulatory activity. | the varicella-zoster virus (vzv) putative immediate-early (ie) protein encoded by orf63 is the homolog of hsv-1 icp-22. to further characterize orf63 and its function, northern analysis, primer extension, and s1 nuclease assays were used to map its transcripts, and transient transfection assays were performed with constructs containing orf63 or its promoter region. two transcripts were identified: a 0.9-kb transcript spans orf63 alone, and a 1.8-kb transcript reads through orf64. two prominent t ... | 1995 | 7747473 |
| pseudorabies virus epo is functionally homologous to varicella-zoster virus orf61 protein and herpes simplex virus type 1 icpo. | pseudorabies virus (prv) early protein o (epo) is the homolog of varicella-zoster virus (vzv) open reading frame 61 (orf61) protein and herpes simplex virus type 1 (hsv-1) icpo. a prv epo deletion mutant grows poorly in cell culture, suggesting that epo plays a critical role in the viral replicative cycle. in this study, we have shown that the growth defect of an epo deletion mutant was complemented in vero cells expressing vzv orf61 protein or hsv-1 icpo. in transient expression assays prv epo, ... | 1995 | 7747481 |
| mutational analysis of varicella-zoster virus major immediate-early protein ie62. | the varicella-zoster virus (vzv) open reading frame 62 encodes an immediate-early protein (ie62) that transactivates expression of various vzv promoters and autoregulates its own expression in transient expression assays. in vero cells, ie62 was shown to transactivate the expression of all putative immediate-early (ie) and early (e) genes of vzv with an up-regulating effect at low intracellular concentrations. to define the functional domains involved in the regulatory properties of ie62, a larg ... | 1995 | 7753624 |
| the transcriptional regulatory proteins encoded by varicella-zoster virus open reading frames (orfs) 4 and 63, but not orf 61, are associated with purified virus particles. | of the five varicella-zoster virus (vzv) open reading frames (orfs) known to encode proteins which influence viral transcriptional events, two (orfs 10 and 62) encode proteins associated with the tegument of virus particles, where they may function during the immediate-early events of infection. in this study, antibodies which recognize the products of the three additional vzv orfs, orfs 4, 61, and 63, were made and used to characterize their association with virus particles. orf 4 encoded a 52- ... | 1995 | 7769688 |
| famciclovir for the treatment of acute herpes zoster: effects on acute disease and postherpetic neuralgia. a randomized, double-blind, placebo-controlled trial. collaborative famciclovir herpes zoster study group. | to document the effects of treatment with famciclovir on the acute signs and symptoms of herpes zoster and postherpetic neuralgia. | 1995 | 7778840 |
| safety and cellular and humoral immune responses of a booster dose of varicella vaccine 6 years after primary immunization. | four hundred nineteen children and adolescents immunized with live varicella vaccine 4-6 years earlier were enrolled in a study to evaluate the safety and immune response to a booster dose containing approximately 3300 pfu of virus. of the subjects, 99% (414/419) maintained antibody to varicella zoster virus (vzv) with a geometric mean titer of 25.7 and mean stimulation index (si) for vzv-specific lymphoproliferation response of 40.3 +/- 5.3 (se). some 7-10 days after the booster immunization, s ... | 1995 | 7797914 |
| varicella: pediatric genital/rectal vesicular lesions of unclear origin. | three children who presented with localized vesicular rash in the diaper area were found to have primary varicella-zoster infections. primary varicella can closely mimic genital herpes simplex virus (hsv 1 or 2), which may be an indicator of sexual abuse. to avoid unfounded investigation for sexual abuse, primary varicella-zoster infection must be included in the differential diagnosis. direct fluorescent antibody testing is a sensitive and specific diagnostic test that can be used to distinguis ... | 1995 | 7802360 |
| importance of concomitant viral infection during late acute liver allograft rejection. | we have determined accompanying events and reviewed the management and outcome of late acute cellular rejection episodes in 384 consecutive liver recipients. a significant proportion of patients experienced concomitant viral infection (group 1, n = 15 [41%]), with cmv infection comprising the largest group and smaller contributions from other viruses (cmv, 30%; hsv, 5%; ebv, 3%; varicella zoster virus, 3%). thirteen (35%) patients (group 2) developed late rejection associated with low maintenanc ... | 1995 | 7839426 |
| licensure of varicella virus vaccine, live. | 1995 | 7898423 | |
| fulminant hepatic failure following varicella-zoster infection in a child. a case report of successful treatment with liver transplantation and perioperative acyclovir. | 1995 | 7491682 | |
| [detection of serum iga antibodies to varicella zoster virus (vzv)--differential etiology of peripheral facial paralysis. a case report]. | during the early manifestation of peripheral facial paralysis, vzv specific iga antibodies were detected in the serum in a single case. in similar cases, this serological parameter should be tested for causal diagnosis in connection with early aciclovir therapy. | 1995 | 7495437 |
| severe chickenpox during treatment with corticosteroids. at what age should varicella-zoster immunoglobulin be given? | 1995 | 7866184 | |
| pharmacology and clinical use of foscarnet. | foscarnet, licenced by astra pharmaceutical products, is a pyrophosphate analogue that selectively inhibits replication of viruses in infected cells. it inhibits in vitro the replication of all herpes viruses, including human cytomegalovirus (hcmv) at concentrations of 100 to 300 mumol/l and has a dose-related inhibitory effect on hiv-1 virus, influenza virus and hepatitis b virus. it does not require intra-cellular phosphorylation for antiviral activity. oral bioavailability of foscarnet is low ... | 1995 | 18611671 |
| diagnosis of varicella-zoster and evaluation of varicella vaccine by passive haemagglutination by non-invasive procedures. | 1994 | 7887029 | |
| comparison of three commercially available assays for detection of varicella-zoster virus antibody. | three commercially available diagnostic assays for the detection of antibodies to varicella-zoster virus were evaluated to determine which would be the most suitable for our clinical laboratory. three different methods were examined: an enzyme-linked immunosorbent assay (elisa), latex agglutination (la), and an indirect fluorescent-antibody technique. for the 141 serum specimens tested, the elisa had an agreement of 90.1% and la had an agreement of 92.2% with the indirect immunofluorescent-antib ... | 1994 | 7496943 |
| characterization of the putative protein kinases specified by varicella-zoster virus genes 47 and 66. | the proteins predicted to be encoded by varicella-zoster virus (vzv) genes 47 and 66 display sequence similarity to the serine/threonine family of protein kinases. homologues of gene 47 exist in alpha-, beta- and gamma-herpesviruses but homologues of gene 66 are specific to the alpha-herpesviruses. monospecific rabbit antisera were raised against two separate fusion proteins constructed from a portion of each protein fused to the carboxy terminus of beta-galactosidase. these antisera were used t ... | 1994 | 8113753 |
| outcome after maternal varicella infection in the first 20 weeks of pregnancy. | infection with the varicella-zoster virus during pregnancy can produce an embryopathy characterized by limb hypoplasia, eye and brain damage, and skin lesions. the risk is greatest when infection occurs during the first 20 weeks of pregnancy, but the magnitude of the risk is uncertain. | 1994 | 8114861 |
| acyclovir resistant varicella zoster and hiv infection. | a child infected with hiv who developed chronic varicella zoster virus infection resistant to acyclovir is presented. the clinical course of the infection, treatment, virological investigations, and relationship of the infection to the child's immunodeficient state are discussed. | 1994 | 8129436 |
| a novel immunosensor for herpes viruses. | we have developed a reusable piezoelectric immunosensor for the detection of the following human herpes viruses: herpes simplex viruses types 1 and 2, varicella-zoster virus, epstein-barr virus, and cytomegalovirus. synthetic peptides, representing different surface antigens of the five viruses, were used to generate virus-specific monoclonal antibodies. apply an antibody layer via protein a immobilization onto a 10-mhz at-cut crystal resulted in 5 x 10(4)-1 x 10(9) viruses on the electrode surf ... | 1994 | 8135375 |
| clinical application of responder cell frequency estimates with four years of follow up. | limiting dilution cultures have been used to estimate the frequency of t cells which respond to antigen stimulation in vitro, but nothing is known of the reproducibility of this assay when applied to human blood. we developed a simplified form of limiting dilution culture in which blood mononuclear cells were diluted in round bottom 96-well plates and cultured for 10 days. the assay was used to estimate the frequency of blood mononuclear cells proliferating in response to varicella-zoster virus ... | 1994 | 8157986 |
| varicella-zoster virus (vzv) reactivation is related to the low response of vzv-specific immunity after chickenpox in infancy. | to clarify the cause of herpes zoster in immunocompetent children, specific humoral and cellular immunity was determined using an elisa and a lymphoproliferative assay, respectively, in infants < 1 year of age and children > or = 1 year of age who had chickenpox. thirteen (59.1%) of 22 infants, 17 (81.0%) of 21 children > or = 1 year of age (p < .02), and 13 (86.7%) of 15 children > or = 2 years of age (p < .001) had positive varicella-zoster virus (vzv)-specific cellular immunity. vzv-specific ... | 1994 | 8158043 |
| a rapid simple in situ hybridization method for herpes simplex virus employing a synthetic biotin-labeled oligonucleotide probe: a comparison with immunohistochemical methods for hsv detection. | we examined 28 paraffin-embedded tissue specimens with histologic evidence of herpes virus infection by in situ hybridization (ish) utilizing manual capillary action technology (microprobe staining system) and a 21 base synthetic multibiotinylated oligonucleotide probe from the hsv glycoprotein c region. the results were compared to a rapid simple immunohistochemical (ihc) protocol for detection of hsv proteins. hsv was detected by ish and ihc in all but one specimen which was shown to be positi ... | 1994 | 8189321 |
| transcriptional mapping of the varicella-zoster virus regulatory genes encoding open reading frames 4 and 63. | four of the 68 varicella-zoster virus (vzv) unique open reading frames (orfs), i.e., orfs 4, 61, 62, and 63, encode proteins that influence viral transcription and are considered to be positional homologs of herpes simplex virus type 1 (hsv-1) immediate-early (ie) proteins. in order to identify the elements that regulate transcription of vzv orfs 4 and 63, the encoded mrnas were mapped in detail. for orf 4, a major 1.8-kb and a minor 3.0-kb polyadenylated [poly(a)+] rna were identified, whereas ... | 1994 | 8189496 |
| helicase-primase complex of herpes simplex virus type 1: a mutation in the ul52 subunit abolishes primase activity. | the ul52 gene product of herpes simplex virus type 1 (hsv-1) comprises one subunit of a 3-protein helicase-primase complex that is essential for replication of viral dna. the functions of the individual subunits of the complex are not known with certainty, although it is clear that the ul8 subunit is not required for either helicase or primase activity. examination of the predicted amino acid sequence of the ul5 gene reveals the existence of conserved helicase motifs; it seems likely, therefore, ... | 1994 | 8189507 |
| mutation of a single lysine residue severely impairs the dna recognition and regulatory functions of the vzv gene 62 transactivator protein. | the product of varicella-zoster virus gene 62 (vzv 140k) is a potent transactivator protein. we have identified a region within the dna binding domain of vzv 140k that shows a striking similarity to the dna recognition helix of the homeodomain, with an especially highly conserved quartet of residues, wlqn. the 140k protein has functional counterparts within the other alphaherpesviruses, which include the major transcriptional regulatory protein of hsv-1, (icp4), and the wlqn region is highly con ... | 1994 | 7907417 |
| varicella-zoster vaccination for health care workers. | 1994 | 7910351 | |
| severe pneumonia in pregnancy three months after resolution of cutaneous zoster. | a 22 weeks pregnant women was affected by a life-threatening pneumonia and a paresis of the proximal muscles with cerebrospinal fluid pleocytosis. her past medical history had been unremarkable except for recurrent episodes of paraumbilical herpes zoster. the clinical findings suggested a dissemination of varicella-zoster virus without skin lesions. acyclovir was added to the therapy, and the clinical picture began to improve. varicella-zoster virus dna was detected in placental tissue by dna-hy ... | 1994 | 7927822 |
| occurrence of chickenpox during pregnancy in women seropositive for varicella-zoster virus. | in a group of 21 women counseled after exposure to chickenpox during pregnancy, 4 developed varicella despite initial studies showing that preinfection sera were varicella-zoster virus (vzv)-seropositive by fluorescent anti-membrane antibody, latex agglutination, elisa, and vzv glycoprotein immunoblot assay. further investigations showed that 2 of the 4 had low-titer (1/100), low-avidity, vzv-reactive igg3 antibodies by elisas of preinfection sera. after chickenpox, these women developed primary ... | 1994 | 7930746 |
| characterization and immunogenicity of a candidate subunit vaccine against varicella-zoster virus. | this study describes the properties of an inactivated subunit antigen preparation from varicella-zoster virus (vzv)-infected mrc-5 cells by treatment with detergent and formaldehyde, ultracentrifugation over sucrose and acetone precipitation. the method preserved the antigenicity of vzv proteins and several vzv-specific glycoproteins, while virus dna was less than 20 pg/250 micrograms protein--a putative vaccine dose. the vaccine was immunogenic in rabbits and stimulated antibodies to the major ... | 1994 | 7935160 |
| varicella-zoster virus myelitis: an expanding spectrum. | we report four cases of varicella-zoster virus (vzv)-associated myelopathy in adults. myelopathy was remitting-exacerbating in two remarkable instances, once acute and once chronic. vzv myelopathy was diagnosed based on the close temporal relationship between rash and onset of myelopathy, and for the first time, by polymerase chain reaction, which revealed vzv dna in the cerebral spinal fluid of three patients with pleocytosis weeks to months later. magnetic resonance imaging was abnormal in thr ... | 1994 | 7936229 |
| effect of varicella zoster virus antigen-antibody complexes on hydrogen peroxide generation by human polymorphonuclear leukocytes. | hydrogen peroxide (h2o2) generation by human polymorphonuclear leukocytes (pmn) incubated with varicella zoster virus (vzv) antigen was studied by cytofluorography. hydrogen peroxide generation was detected in the presence of vzv-seropositive sera. when seropositive sera were heat-inactivated, h2o2 generation was reduced. when pmn were pre-incubated with leu-11b, a monoclonal antibody to the fc receptor on pmn, h2o2 generation was also reduced. these results suggest that vzv antigen-antibody-com ... | 1994 | 7941996 |
| chronic verrucous varicella-zoster infection in a patient with aids. | the expression of herpes varicella zoster virus in patients already infected with the human immunodeficiency virus (hiv) provides the perfect illustration of an opportunist microbe at work. there is an increased incidence of herpes zoster which may be more severe, recurrent or disseminated. likewise, varicella may be more severe and recurrent. in both patterns of infection atypical lesions in the form of persistent ulcerative or verrucous lesions have been seen. an hiv-positive patient is descri ... | 1994 | 7955476 |
| mapping, cloning and sequencing of a glycoprotein-encoding gene from bovine herpesvirus type 1 homologous to the ge gene from hsv-1. | in order to map and identify the glycoprotein-encoding gene from bovine herpesvirus type 1 (bhv-1), homologous to the ge glycoprotein from herpes simplex virus type 1 (hsv-1), a region of the unique short sequence from the bhv-1 genome has been sequenced. the sequenced region contains an orf coding for a polypeptide of 575 amino acids (aa). the aa sequence presents substantial similarity to that of the glycoprotein ge from hsv-1 and to homologous proteins of related viruses such as pseudorabies ... | 1994 | 7958994 |
| delineation of a sequence required for nuclear localization of the protein encoded by varicella-zoster virus gene 61. | all characterized alphaherpesviruses encode a protein whose n-terminal region contains a novel zinc-binding motif, the c3hc4 domain. homology between the different proteins is in general limited to key residues in this domain. in order to identify a separate landmark site in the c3hc4 protein encoded by varicella-zoster virus gene 61, namely the region required for nuclear localization, we have analysed a range of mutants in transient expression and immunofluorescence experiments. a basic region ... | 1994 | 7964633 |
| vitreous fluid sampling and viral genome detection for the diagnosis of viral retinitis in patients with aids. | cytomegalovirus (cmv) causes severe necrotizing retinitis in patients with the acquired immune deficiency syndrome (aids) and other herpesviruses have been implicated in the acute retinal necrosis syndrome (arn), seen in both the immunocompetent and the immunosuppressed. at present the diagnosis of viral retinitis relies solely on clinical appearances. in order to assess whether the detection of herpesvirus-specific dna in cell-free vitreous biopsy samples could be useful in the early diagnosis ... | 1994 | 7964643 |
| varicella-zoster virus (vzv) open reading frame 10 protein, the homolog of the essential herpes simplex virus protein vp16, is dispensable for vzv replication in vitro. | varicella-zoster virus (vzv) open reading frame 10 (orf10) protein in the homolog of the herpes simplex virus type 1 (hsv-1) protein vp16. vzv orf10 transactivates the vzv ie62 gene and is a tegument protein present in the virion. hsv-1 vp16, a potent transactivator of hsv-1 immediate-early genes and tegument protein, is essential for hsv-1 replication in vitro. to determine whether vzv orf10 is required for viral replication in vitro, we constructed two vzv mutants which were unable to express ... | 1994 | 7966575 |
| the ring finger domain of the varicella-zoster virus open reading frame 61 protein is required for its transregulatory functions. | varicella-zoster virus (vzv) open reading frame 61 (orf61) protein transactivates expression of vzv promoters. vzv orf61 is functionally homologous to herpes simplex virus type 1 (hsv-1)-infected cell protein 0 (icp0); however, amino acid sequence homology of these two proteins is limited to the ring finger domains, a recently identified sequence motif composed of cysteine and histidine residues, located in their amino-terminal regions. a carboxy-terminal truncation mutant of icp0 (which retains ... | 1994 | 7975220 |
| down-regulation of glial fibrillary acidic protein expression during acute lytic varicella-zoster virus infection of cultured human astrocytes. | the effects of the varicella-zoster virus (vzv) oka vaccine strain in producing morphologic and antigenic changes in dissociated cultures of human fetal brain was investigated. cultures containing 80% glial acidic fibrillary protein (gfap), gfap+ (positive) astrocytes and 20% gfap- (negative) fibroblastic-like cells were infected with cell-free vzv oka at a multiplicity of infection of 0.1 plaque-forming units per cell. cytopathic effects and significant viral antigen labeling with antibodies ag ... | 1994 | 7975257 |
| [asymptomatic varicella infections in pregnancy: detection and problems]. | two cases of asymptomatic varicella infection during pregnancy will be presented. the diagnosis was made by the evidence of varicella-zoster-virus (vzv)-specific igm or by the significant increase in antibody titer. the incidence of such subclinical vzv infections must be the issue of further investigations. because of the rare occurrence of an embryopathy no general screening is usually made. on the other hand serological testing is recommended in cases of vzv infection in the surroundings of a ... | 1994 | 7987086 |
| [bilateral acute retinal artery necrosis--healing of the second affected eye]. | the acute retinal necrosis syndrome (arn) is caused by the varicella zoster virus or the herpes simplex virus. however the dosage and duration of the antiviral therapy for prevention of an infection in the second eye or treatment of an infection on an affected fellow eye is still not known. we discuss the timing of a possible steroid treatment and demonstrate in a case report how an acute retinal necrosis syndrome in a fellow eye was successfully treated. | 1994 | 8022154 |
| acute retinal necrosis caused by reactivation of herpes simplex virus type 2. | acute retinal necrosis is a severe form of necrotizing retinitis. acute retinal necrosis has been demonstrated to be caused by varicella-zoster virus and herpes simplex virus type 1. we treated three patients with acute retinal necrosis apparently caused by recrudescence of latent herpes simplex virus type 2. primary viral infection was probably congenital, with documented perinatal herpes simplex virus type 2 infection in two patients. bilateral chorioretinal scars were present in two patients, ... | 1994 | 8053466 |
| controversies in pediatric dermatology. | 1994 | 8060737 | |
| vaccines for varicella-zoster virus and cytomegalovirus: recent progress. | 1994 | 8073277 | |
| effect of glycyrrhizin in children with liver dysfunction associated with cytomegalovirus infection. | glycyrrhizin (gl) has an inhibitory effect on several viruses including human immunodeficiency virus type 1 (hiv-1) and varicella-zoster virus (vzv). in addition, some therapeutic and prophylactic effects on chronic active viral hepatitis have been claimed for gl. in this study, 0.2% gl dissolved in saline (2 mg/ml gl), supplemented with 2% glycine and 0.1% cysteine (stronger neo-minophagen c, snmc) was administered intravenously in a dose of 50 ml/day for a period of more than one week to three ... | 1994 | 8073426 |
| development of myelopathy before herpes zoster rash in a patient with aids. | we describe the case of a patient with aids who developed progressive myelopathy due to varicella-zoster virus 2 months before the appearance of skin lesions typical of herpes zoster. varicella-zoster virus was isolated from his csf. therapy with acyclovir failed to control his neurological complications despite its in vitro efficacy against the isolates. | 1994 | 8075278 |
| varicella-zoster virus antibody avidity and igg-subclass patterns in children with recurrent chickenpox. | the normal immune response after primary varicella-zoster virus (vzv) infection includes igg subclass evolution to predominantly igg1-type antibodies, and maturation from low to high avidity antibodies which are maintained for life. twenty-three healthy and apparently immunocompetent children with a history of 2-5 episodes of chickenpox were studied after repeat disease. serial sera were tested for vzv-igg subclass patterns and vzv igg and g-subclass antibody avidity by urea elution enzyme linke ... | 1994 | 8083659 |
| identification and characterization of marek's disease virus genes homologous to icp27 and glycoprotein k of herpes simplex virus-1. | we have identified two marek's disease virus (mdv) genes within the ecori-b fragment of mdv-ga genomic dna. ecori-b is 11.3-kb long and maps within the long unique region of mdv genomes. a 3.2-kb fragment of ecori-b has been sequenced and contains two open reading frames, orf53 and orf54. orf53 (mdv gk), a homolog to hsv-1 glycoprotein k (gk), is 1062 nucleotides long and encodes 354 amino acids (39.5 kda). orf54, designated mdv icp27, based on significant similarity to hsv-1 icp27, is 1419 nucl ... | 1994 | 8091655 |
| molecular evolution of herpesviruses: genomic and protein sequence comparisons. | phylogenetic reconstruction of herpesvirus evolution is generally founded on amino acid sequence comparisons of specific proteins. these are relevant to the evolution of the specific gene (or set of genes), but the resulting phylogeny may vary depending on the particular sequence chosen for analysis (or comparison). in the first part of this report, we compare 13 herpesvirus genomes by using a new multidimensional methodology based on distance measures and partial orderings of dinucleotide relat ... | 1994 | 8107249 |
| effects of human alpha, beta and gamma interferons on varicella zoster virus in vitro. | the antiviral effects of interferon (ifn) on varicella zoster virus (vzv) and herpes simplex virus (hsv) in vitro were examined. the values for the 50% inhibitory dose (id50) of ifn-alpha, beta and gamma determined by plaque reduction assay, were 0.813, 0.650 and 13.750 iu/ml, respectively, against vzv and 18.00, 10.38 and 115.0 iu/ml, respectively, against hsv. thus ifn-alpha and beta were more effective than ifn-gamma against both vzv and hsv and vzv was more sensitive than hsv to the ifns. fi ... | 1994 | 7855636 |
| the n-7-substituted acyclic nucleoside analog 2-amino-7-[(1,3-dihydroxy-2-propoxy)methyl]purine is a potent and selective inhibitor of herpesvirus replication. | 2-amino-7-[(1,3-dihydroxy-2-propoxy)methyl]purine (compound s2242) represents the first antivirally active nucleoside analog with the side chain attached to the n-7 position of the purine ring. compound s2242 strongly inhibits the in vitro replication of both herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) (50% effective concentration [ec50], 0.1 to 0.2 microgram/ml), varicella-zoster virus (ec50, 0.01 to 0.02 microgram/ml) and thymidine kinase (tk)-deficient strains of hsv (ec50, 0.4 mic ... | 1994 | 7695251 |
| wegener's granulomatosis, pituitary adenoma and barn. | a 53-year-old man with wegener's granulomatosis and a co-existing pituitary adenoma developed bilateral acute retinal necrosis (barn), probably secondary to varicella-zoster virus (vzv) infection as igm antibodies were detected in the serum. intravenous acyclovir and ganciclovir limited the spread of necrosis, but to prevent recurrence he was maintained on oral acyclovir. a left cataract developed 17 months later which was extracted and replaced with a heparin surface modified intraocular lens. ... | 1994 | 7642338 |
| decreased incidence of viral infections in liver transplant recipients. possible effects of fk506? | cytomegalovirus (cmv) is a major infectious complication of organ transplantation and its incidence is influenced by the type and intensity of immunosuppressive therapy employed. using a new immunosuppressive agent fk506, cmv infection was observed in 30% and cmv disease in 15% of the 26 liver transplant recipients. delayed onset of cmv disease was noted; the mean time to the occurrence of cmv disease being 137 days posttransplantation. no graft loss or mortality could be attributed to cmv infec ... | 1994 | 7506641 |
| aciclovir. a reappraisal of its antiviral activity, pharmacokinetic properties and therapeutic efficacy. | aciclovir (acyclovir) is a nucleoside analogue with antiviral activity in vitro against the herpes simplex viruses (hsv), varicella zoster virus (vzv), epstein-barr virus (ebv), cytomegalovirus (cmv) and human herpesvirus 6 (hhv-6). topical, oral or intravenous aciclovir is well established in the treatment of ophthalmic, mucocutaneous and other hsv infections, with intravenous aciclovir the accepted treatment of choice in herpes simplex encephalitis. the efficacy of aciclovir is increased with ... | 1994 | 7510619 |
| antibody-binding sites on truncated forms of varicella-zoster virus gpi(ge) glycoprotein. | varicella-zoster virus (vzv)-seropositive human sera were shown to be reactive with the truncated vzv gpi(ge) candidate subunit vaccine (tgpi-511). to identify the location of antibody-binding sites (epitopes) on tgpi-511, three truncated forms of tgpi-511 glycoprotein (tgpi-124, tgpi-160, tgpi-316) dna encoding the n-terminal region of this glycoprotein with amino acid residues of 124, 160 and 360, respectively, were inserted into the vaccinia virus genome. infection of cells with recombinant v ... | 1994 | 7531921 |
| human herpesviruses: research, and threats to health professionals. | 1994 | 7489873 | |
| detection of varicella zoster virus dna by polymerase chain reaction in clinical samples from patients with hunt's syndrome. | 1994 | 10774415 | |
| inflammatory diseases of the mucous membranes. part 1. viral and fungal infections. | with the advent of modern molecular biology and advances in immunology, the pathogenesis of oral mucosal disease processes has become significantly more clear, allowing reliable diagnostic criteria and more precisely targeted management strategies. this paper reviews current findings relative to pathogenesis, diagnosis and treatment of viral and fungal infections of the oral mucous membranes. | 1994 | 8598526 |
| detection of varicella-zoster virus dna in conjunctivas of patients with herpes zoster. | detection of varicella-zoster virus (vzv) dna was carried out in the conjunctivas of 18 patients with herpes zoster by polymerase chain reaction. the rate of vzv dna detection in conjunctivas was 3/4 in herpes zoster ophthalmicus associated with eruption on the dorsum nasi, 2/5 in herpes zoster ophthalmicus without eruption on the dorsum nasi, 1/2 in herpes zoster with eruption on the cheek and lower jaw, 1/2 in generalized herpes zoster and 0/5 in herpes zoster associated with eruption on the t ... | 1994 | 8145984 |
| regio- and stereoselective synthesis of carbocyclic 2',3'-dideoxy-3'-fluoro nucleosides as potential antiviral agents. | the synthesis and antiviral activity of racemic carbocyclic 2',3'-dideoxy-3'-fluoro nucleosides are reported. carbocyclic 2',3'-dideoxy-3'-fluoro nucleosides were obtained from the 3-fluoro cyclopentane derivative 4, which was prepared by two methods. the sn2-displacement of the hydroxyl group of (+/-)-(1 beta, 2 alpha, 3 beta, 4 beta)-4-acetamido-2-fluoro-3-hydroxycyclopentylmethyl acetate (1) with ph3p-i2 followed by tin hydride reduction afforded the 3-fluoroamino alcohol derivative 3. altern ... | 1994 | 8149453 |
| photodynamic inactivation of herpes viruses with phthalocyanine derivatives. | the antiviral photosensitization capacity of 11 different phthalocyanine (pc) derivatives was examined using herpes simplex virus-1, herpes simplex virus-2 and varicella zoster virus in the search for the most potent sensitizers for viral decontamination of blood. the kinetics of viral photoinactivation were resolved during the stages of viral adsorption and penetration into the host cells. the capacity of pc in the photodynamic inactivation of viruses was compared with that of merocyanine 540 ( ... | 1994 | 8151454 |
| deletion of the varicella-zoster virus large subunit of ribonucleotide reductase impairs growth of virus in vitro. | cells infected with varicella-zoster virus (vzv) express a viral ribonucleotide reductase which is distinct from that present in uninfected cells. vzv open reading frames 18 and 19 (orf18 and orf19) are homologous to the herpes simplex virus type 1 genes encoding the small and large subunits of ribonucleotide reductase, respectively. we generated recombinant vzv by transfecting cultured cells with four overlapping cosmid dnas. to construct a virus lacking ribonucleotide reductase, we deleted 97% ... | 1994 | 8151792 |
| varicella-zoster virus retinitis as the initial manifestation of the acquired immunodeficiency syndrome. | 1994 | 8154542 | |
| functional roles of terminal glycomoieties in varicella-zoster virus infection. | terminal glycomoieties of varicella-zoster virus glycoproteins were characterized by their reactivity with lectins and glycosidases, and the functional roles of terminal sugars were analyzed by cell-free virus infectivity. terminal glycan structures of gpl possessed sialic acid linked alpha (2-3) to galactose of o-linked glycan and galactose-beta (1-4)-n-acetylglucosamine of n-linked glycan. those of the putative gpiv possessed galactose-beta (1-4)-n-acetylglucosamine of n-linked glycan. both gl ... | 1994 | 8259682 |
| varicella in children with perinatally acquired human immunodeficiency virus infection. | thirteen children with human immunodeficiency virus infection acquired perinatally and with varicella were identified. clinical and epidemiologic information, including the use of varicella immune globulin and acyclovir, was obtained and testing for antibodies to varicella-zoster virus was done. the 13 children infected with human immunodeficiency virus had an uncomplicated clinical course, and many had a significant antibody response to varicella-zoster virus. | 1994 | 8301436 |
| cloning and sequencing of genomic dna extracted from archival human temporal bone sections. | cloning techniques allow the engineering and production of highly purified dna. further advances in molecular biology have provided the means to identify dna sequences in a rapid fashion. sequencing methods can identify mutations, deletions, polymorphisms, or confirm a known genetic sequence. the use of these techniques in clinical medicine has made it possible to accurately diagnose infectious diseases and determine the molecular etiology of many genetic disorders and malignancies. in this stud ... | 1994 | 8302113 |