Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| bacillus volatiles adversely affect the physiology and ultra-structure of ralstonia solanacearum and induce systemic resistance in tobacco against bacterial wilt. | volatile organic compounds (vocs) produced by various bacteria have significant potential to enhance plant growth and to control phytopathogens. six of the most effective antagonistic bacillus spp. were used in this study against ralstonia solanacearum (rsc) tbbs1, the causal agent of bacterial wilt disease in tobacco. bacillus amyloliquefaciens fzb42 and bacillus artrophaeus lssc22 had the strongest inhibitory effect against rsc. thirteen vocs produced by fzb42 and 10 by lssc22 were identified ... | 2017 | 28091587 |
| development of a sensitive and specific polyclonal antibody for serological detection of clavibacter michiganensis subsp. sepedonicus. | the quarantine bacterium clavibacter michiganensis subsp. sepedonicus (cms) causes bacterial ring rot (brr) in potato but is difficult to detect, hampering the diagnosis of this disease. elisa immunoassays have not been widely used to detect cms because commercially available anti-cms antibodies detect mainly eps-producing bacteria and can fail to detect strains that do not produce eps. in the current study, we developed a new type of polyclonal antibody that specifically detects clavibacter mic ... | 2017 | 28068400 |
| characterization of novel acidobacteria exopolysaccharides with potential industrial and ecological applications. | acidobacteria have been described as one of the most abundant and ubiquitous bacterial phyla in soil. however, factors contributing to this ecological success are not well elucidated mainly due to difficulties in bacterial isolation. acidobacteria may be able to survive for long periods in soil due to protection provided by secreted extracellular polymeric substances that include exopolysaccharides (epss). here we present the first study to characterize epss derived from two strains of acidobact ... | 2017 | 28117455 |
| molecular evidence for the involvement of a polygalacturonase-inhibiting protein, ghpgip1, in enhanced resistance to verticillium and fusarium wilts in cotton. | polygalacturonase-inhibiting protein (pgip), belonging to a group of plant defence proteins, specifically inhibits endopolygalacturonases secreted by pathogens. herein, we showed that purified ghpgip1 is a functional inhibitor of verticillium dahliae and fusarium oxysporum f. sp. vasinfectum, the two fungal pathogens causing cotton wilt. transcription of ghpgip1 was increased in cotton upon infection, wounding, and treatment with defence hormone and h2o2. resistance by ghpgip1 was examined by it ... | 2017 | 28079053 |
| foliar application of the leaf-colonizing yeast pseudozyma churashimaensis elicits systemic defense of pepper against bacterial and viral pathogens. | yeast associates with many plant parts including the phyllosphere, where it is subject to harsh environmental conditions. few studies have reported on biological control of foliar pathogens by yeast. here, we newly isolated leaf-colonizing yeasts from leaves of field-grown pepper plants in a major pepper production area of south korea. the yeast was isolated using semi-selective medium supplemented with rifampicin to inhibit bacterial growth and its disease control capacity against xanthomonas a ... | 2017 | 28071648 |
| ralstonia picketti neonatal sepsis: a case report. | ralstonia genus are gram negative bacillus and includes four bacteria namely ralstonia picketti, ralstonia solanacearum, ralstonia insidiosa and ralstonia mannitolilytica. these are opportunistic pathogens and cause infections in immunocompromised host. the sources of infection are usually contaminated solutions and water. the majority of the reported cases are caused by r. picketti. it is very rare cause of neonatal sepsis with less than twenty cases reported in literature till date. | 2017 | 28061799 |
| comparative genomics of host adaptive traits in xanthomonas translucens pv. graminis. | xanthomonas translucens pathovars differ in their individual host ranges among poaceae. as the causal agent of bacterial wilt in italian ryegrass (lolium multiflorum lam.), x. translucens pv. graminis (xtg) is one of the most important bacterial pathogens in temperate grassland regions. the genomes of six xtg strains from switzerland, norway, and new zealand were sequenced in order to gain insight into conserved genomic traits from organisms covering a wide geographical range. subsequent compara ... | 2017 | 28056815 |
| microbial taxa and functional genes shift in degraded soil with bacterial wilt. | soil degradation is a serious global problem, but little is known about how soil microbial communities respond to soil degradation as well as their feedback to ecosystem functioning. in this study, we found the microbial community composition, structure and functional potential significantly altered in the degraded soils with bacterial wilt (termed as degraded soils). compared with healthy soils, otu richness of beneficial microorganisms were significantly decreased, but otu richness of pathogen ... | 2017 | 28051173 |
| the expanding role of type ii secretion in bacterial pathogenesis and beyond. | type ii secretion (t2s) is one means by which gram-negative pathogens secrete proteins into the extracellular milieu and/or host organisms. based upon recent genome sequencing, it is clear that t2s is largely restricted to the proteobacteria, occurring in many, but not all genera in the α-, β-, γ-, and δ- classes. prominent human and/or animal pathogens that express a t2s system(s) include acinetobacter baumannii, burkholderia pseudomallei, chlamydia trachomatis, escherichia coli, klebsiella pne ... | 2017 | 28264910 |
| parasites and competitors suppress bacterial pathogen synergistically due to evolutionary trade-offs. | parasites and competitors are important for regulating pathogen densities and subsequent disease dynamics. it is, however, unclear to what extent this is driven by ecological and evolutionary processes. here, we used experimental evolution to study the eco-evolutionary feedbacks among ralstonia solanacearum bacterial pathogen, ralstonia-specific phage parasite, and bacillus amyloliquefaciens competitor bacterium in the laboratory and plant rhizosphere. we found that while the phage had a small e ... | 2017 | 27925169 |
| psc-afp from psoralea corylifolia l. overexpressed in pichia pastoris increases antimicrobial activity and enhances disease resistance of transgenic tobacco. | psc-afp, isolated from the seeds of psoralea corylifolia l., is an antimicrobial protein with trypsin inhibitor activity. its encoding gene was cloned by 3'- rapid amplification of cdna ends (race) combined with y-shaped adaptor-dependent extension (yade) method. the gene psc-afp encodes a protein of 203 amino acids with a deduced signal peptide of 24 residues. the growth inhibition effect exerted by the heterologously expressed psc-afp in pichia pastoris revealed that the recombinant psc-afp in ... | 2017 | 27587300 |
| physicochemical and antimicrobial properties of copaiba oil: implications on product quality control. | the copaiba oil is a common natural product used in cosmetic industry and as a nutraceutical product. however, lack of quality control and scarce knowledge about its antimicrobial activity is a point of concern. the proposal of this study was to investigate the physicochemical properties and the antimicrobial activity of five commercial brands of copaiba oil. | 2017 | 28068029 |
| growth of bacterial phytopathogens in animal manures. | animal manures are routinely applied to agricultural lands to improve crop yield, but the possibility to spread bacterial phytopathogens through field fertilization has not been considered yet. we monitored 49 cattle, horse, swine, sheep or chicken manure samples collected in 14 polish voivodeships for the most important plant pathogenic bacteria - ralstonia solanacearum (rsol), xanthomonas campestris pv. campestris (xcc), pectobacterium carotovorum subsp. carotovorum (pcc), pectobacterium atros ... | 2017 | 28319994 |
| native and foreign proteins secreted by the cupriavidus metallidurans type ii system and an alternative mechanism. | the type ii secretion system (t2ss), which transports selected periplasmic proteins across the outer membrane, has rarely been studied in nonpathogens or in organisms classified as betaproteobacteria. therefore, we studied cupriavidus metallidurans (cme), a facultative chemilithoautotroph. gel analysis of extracellular proteins revealed no remarkable differences between wild type and t2ss mutants. however, enzyme assays revealed that native extracellular alkaline phosphatase is a t2ss substrate, ... | 2017 | 28119513 |
| analyses of natural variation indicates that the absence of rps4/rrs1 and amino acid change in rps4 cause loss of their functions and resistance to pathogens. | a pair of arabidopsis thaliana resistance proteins, rps4 and rrs1, recognizes the cognate avr effector from the bacterial pathogens pseudomonas syringae pv. tomato expressing avrrps4 (pst-avrrps4), ralstonia solanacearum, and the fungal pathogen colletotrichum higginsianum and leads to defense signaling activation against the pathogens. in the present study, we analyzed 14 a. thaliana accessions for natural variation in pst-avrrps4 and c. higginsianum susceptibility, and found new compatible and ... | 2017 | 28277970 |
| complete genome sequence of ralstonia solanacearum fjat-1458, a potential biocontrol agent for tomato wilt. | an avirulent strain of ralstonia solanacearum fjat-1458 was isolated from a living tomato. here, we report the complete r. solanacearum fjat-1458 genome sequence of 6,059,899 bp and 5,241 genes. this bacterial strain is a potential candidate as a biocontrol agent in the form of a plant vaccine for bacterial wilt. | 2017 | 28385834 |
| concurrent drought stress and vascular pathogen infection induce common and distinct transcriptomic responses in chickpea. | chickpea (cicer arietinum); the second largest legume grown worldwide is prone to drought and various pathogen infections. these drought and pathogen stresses often occur concurrently in the field conditions. however, the molecular events in response to that are largely unknown. the present study examines the transcriptome dynamics in chickpea plants exposed to a combination of water-deficit stress and ralstonia solanacearum infection. r. solanacearum is a potential wilt disease causing pathogen ... | 2017 | 28382041 |
| transcriptomes of ralstonia solanacearum during root colonization of solanum commersonii. | bacterial wilt of potatoes-also called brown rot-is a devastating disease caused by the vascular pathogen ralstonia solanacearum that leads to significant yield loss. as in other plant-pathogen interactions, the first contacts established between the bacterium and the plant largely condition the disease outcome. here, we studied the transcriptome of r. solanacearum uy031 early after infection in two accessions of the wild potato solanum commersonii showing contrasting resistance to bacterial wil ... | 2017 | 28373879 |
| microbial community composition is related to soil biological and chemical properties and bacterial wilt outbreak. | soil microbes play important roles in plant growth and health. little is known about the differences of soil microbes between healthy and bacterial wilt infected soils with ralstonia solanacearum. by illumina-miseq sequencing of 16s rrna and 18s rrna gene amplicons, we found the soil microbial composition and diversity were distinct between healthy and bacterial wilt infected soils. soil microbial community varied at different plant growth stages due to changes of root exudates composition and s ... | 2017 | 28336973 |
| corrigendum: loop-mediated isothermal amplification method for the rapid detection of ralstonia solanacearum phylotype i mulberry strains in china. | [this corrects the article on p. 76 in vol. 8, pmid: 28197157.]. | 2017 | 28280502 |
| the ralstonia solanacearum effector ripak suppresses plant hypersensitive response by inhibiting the activity of host catalases. | the destructive bacterial pathogen ralstonia solanacearum delivers effector proteins via a type-iii secretion system for its pathogenesis of plant hosts. however, the biochemical functions of most of these effectors remain unclear. ripak of r. solanacearum gmi1000 is a type-iii effector with unknown functions. functional analysis demonstrated that in tobacco leaves, ripak knockout bacteria produced an obvious hypersensitive response; also, infected tissues accumulated reactive oxygen species in ... | 2017 | 28252830 |
| resource availability modulates biodiversity-invasion relationships by altering competitive interactions. | community diversity affects the survival of newly introduced species via resource competition. competitive interactions can be modulated by resource availability and we hypothesized that this may alter biodiversity-invasion relationships. to study this, we assessed the growth of a bacterial invader, ralstonia solanacearum, when introduced into communities comprised of one to five closely related resident species under different resource concentrations. the invader growth was then examined as a f ... | 2017 | 28229529 |
| loop-mediated isothermal amplification method for the rapid detection of ralstonia solanacearum phylotype i mulberry strains in china. | ralstonia solanacearum phylotype i mulberry strains are causative agent of bacterial wilt of mulberry. current diagnostic methods are not adopted to the mulberry wilt disease. in this study, we developed a rapid method, loop-mediated isothermal amplification (lamp), to detect r. solanacearum phylotype i mulberry strains. a set of six primers was designed to target the clone mg67 sequence in this lamp detection which can be completed in 20 min at 64°c. the results of the lamp reaction could be ob ... | 2017 | 28197157 |
| diverse microbial communities in non-aerated compost teas suppress bacterial wilt. | non-aerated compost teas (ncts) are water extracts of composted organic materials and are used to suppress soil borne and foliar disease in many pathosystems. greenhouse trials were used to test the effectiveness of ncts to suppress potato bacterial wilt caused by ralstonia solanacearum on plants grown in soils inoculated with a virulent isolate of the pathogen (biovar ii). ncts prepared from matured compost sources: agricultural waste (awct), vermicompost (vct) and solid municipal waste (smwct) ... | 2017 | 28181116 |
| overexpression of a novel peanut nbs-lrr gene ahrrs5 enhances disease resistance to ralstonia solanacearum in tobacco. | bacterial wilt caused by ralstonia solanacearum is a ruinous soilborne disease affecting more than 450 plant species. efficient control methods for this disease remain unavailable to date. this study characterized a novel nucleotide-binding site-leucine-rich repeat resistance gene ahrrs5 from peanut, which was up-regulated in both resistant and susceptible peanut cultivars in response to r. solanacearum. the product of ahrrs5 was localized in the nucleus. furthermore, treatment with phytohormone ... | 2017 | 27311738 |
| genome sequencing of four strains of phylotype i, ii and iv of ralstonia solanacearum that cause potato bacterial wilt in india. | ralstonia solanacearum is a heterogeneous species complex causing bacterial wilts in more than 450 plant species distributed in 54 families. the complexity of the genome and the wide diversity existing within the species has led to the concept of r. solanacearum species complex (rssc). here we report the genome sequence of the four strains (rs2, rs25, rs48 and rs75) belonging to three of the four phylotypes of r. solanacearum that cause potato bacterial wilt in india. the genome sequence data wo ... | 2017 | 28041840 |
| new multilocus variable-number tandem-repeat analysis (mlva) scheme for fine-scale monitoring and microevolution-related study of ralstonia pseudosolanacearum phylotype i populations. | bacterial wilt caused by the ralstonia solanacearum species complex (rssc) is considered one of the most harmful plant diseases in the world. special attention should be paid to r. pseudosolanacearum phylotype i due to its large host range, its worldwide distribution, and its high evolutionary potential. so far, the molecular epidemiology and population genetics of this bacterium are poorly understood. until now, the genetic structure of the rssc has been analyzed on the worldwide and regional s ... | 2017 | 28003195 |
| involvement of ralfuranones in the quorum sensing signalling pathway and virulence of ralstonia solanacearum strain oe1-1. | the soil-borne, plant-pathogenic ralstonia solanacearum strain oe1-1 produces and secretes methyl 3-hydroxymyristate (3-oh mame) as a quorum sensing (qs) signal, which contributes to its virulence. a global virulence regulator, phca, functioning through the qs system, positively regulates the expression of rala, which encodes furanone synthase, to produce aryl-furanone secondary metabolites, ralfuranones. a ralfuranone-deficient mutant (δrala) is weakly virulent when directly inoculated into tom ... | 2017 | 28116815 |
| ralstonia solanacearum differentially colonizes roots of resistant and susceptible tomato plants. | ralstonia solanacearum is the causal agent of bacterial wilt and infects over 200 plant species in 50 families. the soilborne bacterium is lethal to many solanaceous species, including tomato. although resistant plants can carry high pathogen loads (between 10(5) and 10(8) cfu/g fresh weight), the disease is best controlled by the use of resistant cultivars, particularly resistant rootstocks. how these plants have latent infections yet maintain resistance is not clear. r. solanacearum first infe ... | 2017 | 28112595 |
| disruption of coma homolog in ralstonia solanacearum does not impair its twitching motility. | ralstonia solanacearum is an important phyto-pathogenic bacterium. the bacterium exhibits type iv pili meditated twitching motility that has been implicated in the process of natural transformation in it. a coma gene homolog, alike in several other naturally competent bacteria, has been already reported in this bacterium. however, there are no report of direct link between coma and twitching motility during the natural transformation process in this pathogen. in order to figure out any connectio ... | 2017 | 28083961 |
| draft genome sequence of highly virulent race 4/biovar 3 of ralstonia solanacearum cars_mep causing bacterial wilt in zingiberaceae plants in india. | the genome of ralstonia solanacearum cars_mep, a race 4/biovar 3/phylotype i bacterium causing wilt in small cardamom and other zingiberaceae plants, was sequenced. analysis of the 5.7-mb genome sequence will aid in better understanding of the genetic determinants of host range, host jump, survival, pathogenicity, and virulence of race 4 of r. solanacearum. | 2017 | 28057749 |
| cooperative reinforcement of ionic liquid and reactive solvent on enzymatic synthesis of caffeic acid phenethyl ester as an in vitro inhibitor of plant pathogenic bacteria. | it is widely believed that lipases in ionic liquids (ils) possess higher enzyme activity, stability and selectivity; however, reaction equilibrium is always limited by product inhibition, and the product is difficult to separate from non-volatile ils using distillation. to solve this problem, using trialkylphosphine oxide (topo) as a complexing agent, a novel biphase of reactive solvent and il was firstly reported for caffeic acid phenethyl ester (cape) production from methyl caffeate (mc) and 2 ... | 2017 | 28045451 |
| corrigendum: loop-mediated isothermal amplification method for the rapid detection of ralstonia solanacearum phylotype i mulberry strains in china. | [this corrects the article on p. 76 in vol. 8, pmid: 28197157.]. | 2017 | 28405200 |
| comparative genomics identifies a novel conserved protein, hpat, in proteobacterial type iii secretion systems that do not possess the putative translocon protein hrpf. | xanthomonas translucens is the causal agent of bacterial leaf streak, the most common bacterial disease of wheat and barley. to cause disease, most xanthomonads depend on a highly conserved type iii secretion system, which translocates type iii effectors into host plant cells. mutagenesis of the conserved type iii secretion gene hrct confirmed that the x. translucens type iii secretion system is required to cause disease on the host plant barley and to trigger a non-host hypersensitive response ... | 2017 | 28694803 |
| general phytoplasma detection by a q-pcr method using mycoplasma primers. | phytoplasmas and mycoplasmas are bacteria belonging to the class mollicutes. in this study, a fine tuning of quantitative polymerase chain reaction (qpcr) with a universal mycoplasma primer pair (gpo3f/mgso) targeting the 16s rrna gene was carried out on phytoplasmas. the dissociation curves of dnas from catharanthus roseus phytoplasma-infected micropropagated shoots and from phytoplasma field-infected plant samples showed a single peak at 82.5 °c (±0.5) specifically detecting phytoplasmas belon ... | 2017 | 28558918 |
| soil acidification aggravates the occurrence of bacterial wilt in south china. | soil acidification is a major problem in modern agricultural systems and is an important factor affecting the soil microbial community and soil health. however, little is known about the effect of soil acidification on soil-borne plant diseases. we performed a 4-year investigation in south china to evaluate the correlation between soil acidification and the occurrence of bacterial wilt. the results showed that the average soil ph in fields infected by bacterial wilt disease was much lower than t ... | 2017 | 28487678 |
| effect of volatile compounds produced by ralstonia solanacearum on plant growth promoting and systemic resistance inducing potential of bacillus volatiles. | microbial volatiles play an expedient role in the agricultural ecological system by enhancing plant growth and inducing systemic resistance against plant pathogens, without causing hazardous effects on the environment. to explore the effects of vocs of ralstonia solanacearum tbbs1 (rs) on tobacco plant growth and on plant growth promoting efficiency of vocs produced by bacillus subtilis syst2, experiments were conducted both in vitro and in planta. | 2017 | 28768498 |
| bacterial diseases of bananas and enset: current state of knowledge and integrated approaches toward sustainable management. | bacterial diseases of bananas and enset have not received, until recently, an equal amount of attention compared to other major threats to banana production such as the fungal diseases black leaf streak (mycosphaerella fijiensis) and fusarium wilt (fusarium oxysporum f. sp. cubense). however, bacteria cause significant impacts on bananas globally and management practices are not always well known or adopted by farmers. bacterial diseases in bananas and enset can be divided into three groups: (1) ... | 2017 | 28785275 |
| modulation of inter-kingdom communication by phcbsr quorum sensing system in ralstonia solanacearum phylotype i strain gmi1000. | ralstonia solanacearum is a ubiquitous soil-borne plant pathogenic bacterium, which frequently encounters and interacts with other soil cohabitants in competition for environmental niches. ralsolamycin, which is encoded by the rmy genes, has been characterized as a novel inter-kingdom interaction signal that induces chlamydospore development in fungi. in this study, we provide the first genetic evidence that the rmy gene expression is controlled by the phcbsr quorum sensing (qs) system in strain ... | 2017 | 28690607 |
| scmed7, a sugarcane mediator subunit gene, acts as a regulator of plant immunity and is responsive to diverse stress and hormone treatments. | the mediator complex, is an essential component of the rna polymerase ii general transcriptional machinery in eukaryotes. mediator subunit 7 (med7), a key subunit in the central module of this complex, plays an important role in gene transcriptional regulation. the present study isolated the full-length cdna of the med7 gene of sugarcane, hereby designated as scmed7, which was characterized to harbor a 525-bp open reading frame that is predicted to encode a 174-amino acid protein with a molecula ... | 2017 | 28785867 |
| a sugarcane pathogenesis-related protein, scpr10, plays a positive role in defense responses under sporisorium scitamineum, srmv, sa, and meja stresses. | a pathogenesis-related gene, scpr10 , was isolated from sugarcane and its bio-function was characterized, demonstrating that scpr10 was involved in plant defense responses to sporisorium scitamineum , srmv, sa, and meja stresses. plant fungal and viral diseases are the major concerns in sugarcane industry. many anti-fungal and antivirus components, including pathogenesis-related (pr) proteins, have been identified. the pathogenesis-related protein 10 (pr10) is the dominant group in pr families, ... | 2017 | 28634719 |
| wheat rop proteins modulate defense response through lignin metabolism. | rop is a subfamily of small gtp-binding proteins that uniquely exist in plants. it acts as versatile molecular switches that regulate various developmental processes. some rop proteins are also reported to affect defense responses, although their exact mechanism is not fully understood. herein, rop members in wheat were mined; the functions of three wheat rop proteins were studied. rt-pcr results showed that the expression of tarac1 was rapidly and strongly induced after leaf rust infection. tar ... | 2017 | 28716418 |
| isolated pseudomonas aeruginosa strain vih2 and antagonistic properties against ralstonia solanacearum. | the aim of this study was to isolates with antagonist activity against r. solanacearum. thirty-two bacterial isolates were obtained from samples, and they were screened for potential antagonistic activity against r. solanacearum. using the agar spot method, ten out of the 21 tested bacteria showed antilisterial activity. vih2 had the highest inhibitory effect on the growth of r. solanacearum. based on 16s rdna and biolog test analysis, the strain vih2 was identified as pseudomonas aeruginosa. si ... | 2017 | 28847494 |
| a conserved ear motif is required for avirulence and stability of the ralstonia solanacearum effector popp2 in planta. | ralstonia solanacearum is the causal agent of the devastating bacterial wilt disease in many high value solanaceae crops. r. solanacearum secretes around 70 effectors into host cells in order to promote infection. plants have, however, evolved specialized immune receptors that recognize corresponding effectors and confer qualitative disease resistance. in the model species arabidopsis thaliana, the paired immune receptors rrs1 (resistance to ralstonia solanacearum 1) and rps4 (resistance to pseu ... | 2017 | 28824668 |
| direct and indirect visualization of bacterial effector delivery into diverse plant cell types during infection. | to cause disease, diverse pathogens deliver effector proteins into host cells. pathogen effectors can inhibit defense responses, alter host physiology, and represent important cellular probes to investigate plant biology. however, effector function and localization have primarily been investigated after overexpression in planta. visualizing effector delivery during infection is challenging due to the plant cell wall, autofluorescence, and low effector abundance. here, we used a gfp strand system ... | 2017 | 28600390 |
| antibacterial activity of pharbitin, isolated from the seeds of pharbitis nil, against various plant pathogenic bacteria. | this study aimed to isolate and characterize an antibacterial metabolite from pharbitis nil seeds and investigate antibacterial activity against various plant pathogenic bacteria. the methanol extract of p. nil seeds showed the strongest activity against xanthomonas arboricola pv. pruni (xap) with minimum inhibition concentration (mic) value of 250 μg/ml. among the three solvent layers obtained from the methanol extract of p. nil seeds, only butanol layer displayed the activity with mic value of ... | 2017 | 28851207 |
| structure of ralsolamycin, the interkingdom morphogen from the crop plant pathogen ralstonia solanacearum. | ralsolamycin, an inducer of chlamydospore formation in fungi, was recently reported from the plant pathogenic bacterium ralstonia solanacearum. although interpretation of tandem mass data and bioinformatics enabled a preliminary chemical characterization, the full structure of ralsolamycin was not resolved. we now report the recovery of this secondary metabolite from an engineered r. solanacearum strain. the structure of ralsolamycin was elucidated by extensive spectroscopic analyses. chemical d ... | 2017 | 28846435 |
| synthesis and biological evaluation of pyridinium-functionalized carbazole derivatives as promising antibacterial agents. | various pyridinium-functionalized carbazole derivatives were constructed by coupling the key fragments of carbazole skeleton and pyridinium nucleus in a single molecular architecture. antibacterial bioassays revealed that some of the title compounds displayed impressive bioactivities against plant pathogens such as xanthomonas oryzae pv. oryzae, ralstonia solanacearum, and xanthomonas axonopodis pv. citri with minimal ec50 values of up to 0.4, 0.3, and 0.3mg/l, respectively. these bioactivities ... | 2017 | 28843708 |
| cahdz27, a homeodomain-leucine zipper i (hd-zip i) protein, positively regulates the resistance to ralstonia solanacearum infection in pepper. | homeodomain-leucine zipper class i (hd-zip i) transcription factors (tfs) have been functionally characterized in plant responses to abiotic stresses, but their roles in plant immunity are poorly understood. here, a hd-zip i gene, cahz27, was isolated from pepper (capsicum annum) and characterized for its role in pepper immunity. quantitative real-time pcr showed that cahdz27 was transcriptionally induced by ralstonia solanacearum inoculation and exogenous application of methyl jasmonate (meja), ... | 2017 | 28840788 |
| type iii secretion-dependent and -independent phenotypes caused by ralstonia solanacearum in arabidopsis roots. | the causing agent of bacterial wilt, ralstonia solanacearum, is a soilborne pathogen that invades plants through their roots, traversing many tissue layers until it reaches the xylem, where it multiplies and causes plant collapse. the effects of r. solanacearum infection are devastating and no effective approach to fight the disease is so far available. the early steps of infection, essential for colonization, as well as the early plant defense responses, remain mostly unknown. here, we have set ... | 2017 | 28840786 |
| diversity, pathogenicity, and current occurrence of bacterial wilt bacterium ralstonia solanacearum in peru. | the current bacterial wilt infestation level in the potato fields in the peruvian andes was investigated by collecting stem samples from wilted plants and detecting ralstonia solanacearum. in total 39 farmers' fields located in the central and northern peru between the altitudes 2111 and 3742 m above sea level were sampled. r. solanacearum was detected in 19 fields, and in 153 out of the 358 samples analyzed. phylogenetic analysis using the partial sequence of the endoglucanase gene on strains c ... | 2017 | 28769944 |
| bacteriophage-based bacterial wilt biocontrol for an environmentally sustainable agriculture. | bacterial wilt diseases caused by ralstonia solanacearum, r. pseudosolanacearum, and r. syzygii subsp. indonesiensis (former r. solanacearum species complex) are among the most important plant diseases worldwide, severely affecting a high number of crops and ornamentals. difficulties of bacterial wilt control by non-biological methods are related to effectiveness, bacterial resistance and environmental impact. alternatively, a great many biocontrol strategies have been carried out, with the adva ... | 2017 | 28769942 |
| ralstonins a and b, lipopeptides with chlamydospore-inducing and phytotoxic activities from the plant pathogen ralstonia solanacearum. | ralstonia solanacearum has an orphan hybrid polyketide synthase-nonribosomal peptide synthetase gene cluster. we herein isolate its products (named ralstonins a and b) from r. solanacearum and elucidate their structures and biological activities. ralstonins are unusual lipodepsipeptides composed of 11 amino acids (containing unique amino acids such as β-hydroxytyrosine and dehydroalanine) and a 3-amino-2-hydroxyoctadecanoic acid, and their production is controlled by quorum sensing, a mechanism ... | 2017 | 28753290 |
| identification of a gene involved in negative regulation for pyomelanin production in ralstonia solanacearum. | ralstonia solanacearum causes bacterial wilt in a wide variety of host plant species and produces a melanin-like blackish-brown pigment in stationary phase when grown in minimal medium supplemented with tyrosine. to study melanin production regulation in r. solanacearum, five mutants exhibiting over-production of melanin-like pigments were selected from a transposon (tn) insertion mutant library of r. solanacearum sl341. most of the mutants except one (sl341t) were not complemented by the origin ... | 2017 | 28746990 |
| antibacterial activity of tannins isolated from sapium baccatum extract and use for control of tomato bacterial wilt. | in the search for new antibacterial agents from natural sources, we revealed that a crude methanol extract of sapium baccatum was highly active against ralstonia solanacearum, a causal agent of a serious disease called bacterial wilt of tomato. the bioassay-guided fractionation of this extract resulted in the isolation of seven known active compounds, including gallic acid, methyl gallate, corilagin, tercatain, chebulagic acid, chebulinic acid, and quercetin 3-o-α-l-arabinopyranoside. their chem ... | 2017 | 28742863 |
| mechanism of host substrate acetylation by a yopj family effector. | the yersinia outer protein j (yopj) family of bacterial effectors depends on a novel acetyltransferase domain to acetylate signalling proteins from plant and animal hosts. however, the underlying mechanism is unclear. here, we report the crystal structures of popp2, a yopj effector produced by the plant pathogen ralstonia solanacearum, in complex with inositol hexaphosphate (insp6), acetyl-coenzyme a (accoa) and/or substrate resistance to ralstonia solanacearum 1 (rrs1-r)wrky. popp2 recognizes t ... | 2017 | 28737762 |
| ralfuranones contribute to mushroom-type biofilm formation by ralstonia solanacearum strain oe1-1. | after invasion into intercellular spaces of tomato plants, the soil-borne plant pathogenic ralstonia solanacearum strain oe1-1 forms mushroom-shaped biofilms (mushroom-type biofilms, mbfs) on tomato cells, leading to its virulence. the strain oe1-1 produces aryl-furanone secondary metabolites, ralfuranones (a, b, j, k and l), dependent on the quorum sensing (qs) system with methyl 3-hydroxymyristate (3-oh mame) synthesized by phcb as a qs signal. ralfuranones are associated with the feedback loo ... | 2017 | 28722830 |
| exposure to umbelliferone reduces ralstonia solanacearum biofilm formation, transcription of type iii secretion system regulators and effectors and virulence on tobacco. | ralstonia solanacearum is one of the most devastating phytopathogens and causes bacterial wilt, which leads to severe economic loss due to its worldwide distribution and broad host range. certain plant-derived compounds (pdcs) can impair bacterial virulence by suppressing pathogenic factors of r. solanacearum. however, the inhibitory mechanisms of pdcs in bacterial virulence remain largely unknown. in this study, we screened a library of coumarins and derivatives, natural pdcs with fused benzene ... | 2017 | 28713361 |
| ralstonia solanacearum novel e3 ubiquitin ligase (nel) effectors ripaw and ripar suppress pattern-triggered immunity in plants. | ralstonia solanacearum is the causal agent of bacterial wilt in solanaceous crops. this pathogen injects more than 70 effector proteins into host plant cells via the hrp type iii secretion system to cause a successful infection. however, the function of these effectors in plant cells, especially in the suppression of plant immunity, remains largely unknown. in this study, we characterized two ralstonia solanacearum effectors, ripaw and ripar, which share homology with the ipah family of effector ... | 2017 | 28708051 |
| complete factorial design to adjust ph and sugar concentrations in the inoculum phase of ralstonia solanacearum to optimize p(3hb) production. | poly(3-hydroxybutyrate) (p(3hb)) is a biodegradable plastic biopolymer that accumulates as lipophilic inclusions in the cytoplasm of some microorganisms. the biotechnological process by which p(3hb) is synthesized occurs in two phases. the first phase involves cell growth in a complex culture medium, while the second phase involves polymer accumulation in the presence of excess carbon sources. as such, the efficiency of the second phase depends on the first phase. the aim of this study was to ev ... | 2017 | 28704411 |
| hpab-dependent secretion of type iii effectors in the plant pathogens ralstonia solanacearum and xanthomonas campestris pv. vesicatoria. | plant pathogenic bacteria exerts their pathogenicity through the injection of large repertoires of type iii effectors (t3es) into plant cells, a mechanism controlled in part by type iii chaperones (t3cs). in ralstonia solanacearum, the causal agent of bacterial wilt, little is known about the control of type iii secretion at the post-translational level. here, we provide evidence that the hpab and hpad proteins do act as bona fide r. solanacearum class ib chaperones that associate with several t ... | 2017 | 28687734 |
| replications of two closely related groups of jumbo phages show different level of dependence on host-encoded rna polymerase. | ralstonia solanacearum phages φrp12 and φrp31 are jumbo phages isolated in thailand. here we show that they exhibit similar virion morphology, genome organization and host range. genome comparisons as well as phylogenetic and proteomic tree analyses support that they belong to the group of φkz-related phages, with their closest relatives being r. solanacearum phages φrsl2 and φrsf1. compared with φrsl2 and φrsf1, φrp12 and φrp31 possess larger genomes (ca. 280 kbp, 25% larger). the replication o ... | 2017 | 28659872 |
| regulation involved in colonization of intercellular spaces of host plants in ralstonia solanacearum. | a soil-borne bacterium ralstonia solanacearum invading plant roots first colonizes the intercellular spaces of the root, and eventually enters xylem vessels, where it replicates at high levels leading to wilting symptoms. after invasion into intercellular spaces, r. solanacearum strain oe1-1 attaches to host cells and expression of the hrp genes encoding components of the type iii secretion system (t3ss). oe1-1 then constructs t3ss and secrets effectors into host cells, inducing expression of th ... | 2017 | 28642776 |
| genome sequencing of ralstonia solanacearum cqps-1, a phylotype i strain collected from a highland area with continuous cropping of tobacco. | ralstonia solanacearum, an agent of bacterial wilt, is a highly variable species with a broad host range and wide geographic distribution. as a species complex, it has extensive genetic diversity and its living environment is polymorphic like the lowland and the highland area, so more genomes are needed for studying population evolution and environment adaptation. in this paper, we reported the genome sequencing of r. solanacearum strain cqps-1 isolated from wilted tobacco in pengshui, chongqing ... | 2017 | 28620361 |
| tube-wise diagnostic microarray for the multiplex characterization of the complex plant pathogen ralstonia solanacearum. | ralstonia solanacearum is a well-known agricultural and ecological threat worldwide. the complexity of the r. solanacearum species complex (rssc) represents a challenge for the accurate characterization of epidemiological strains by official services and research laboratories. the majority of protocols only focus on a narrow range of strains; however, this species complex includes strains that represent major constraints and are under strict regulation. the main drawback associated with the curr ... | 2017 | 28596774 |
| three draft genome sequences of the bacterial plant pathogen ralstonia solanacearum, isolated in georgia. | ralstonia solanacearum, the causative agent of bacterial wilt, is a devastating bacterial plant pathogen with a wide range of hosts. we report here the first draft genome sequences for three strains of ralstonia solanacearum isolated from infected potato, tomato, and pepper plants in georgia. | 2017 | 28596403 |
| rhizophagus irregularis mucl 41833 transitorily reduces tomato bacterial wilt incidence caused by ralstonia solanacearum under in vitro conditions. | bacterial wilt caused by ralstonia solanacearum is one of the world's most important soil-borne plant diseases. in martinique, french west indies, a highly virulent new pathogenic variant of this bacterium (phylotype iib/4npb) severely impacts tomato production. here we report on the effect of r. solanacearum cfbp 6783, classified in phytotype iib/4npb, on tomato plantlets grown under strict in vitro culture conditions in the presence or absence of the arbuscular mycorrhizal fungus rhizophagus i ... | 2017 | 28585092 |
| preparative separation of tl1-1 from daldinia eschscholzii extract by macroporous resin and evaluation of its antimicrobial activities. | 2,3-dihydro-5-hydroxy-2-methylchromen-4-one (tl1-1) has already been reported to exhibit significant activities such as cytotoxicity, antifungal activity and growth inhibitory activity. in order to simply and efficiently separate tl1-1 from crude extracts of daldinia eschscholzii on a large-preparative scale, xad-16 resin was selected from ten types of resin based on its superior adsorption and desorption performance. adsorption equilibrium data for this resin fitted well with pseudo-first order ... | 2017 | 28582661 |
| multiple disease resistance loci affect soilborne disease resistance in tobacco (nicotiana tabacum). | phytophthora nicotianae and ralstonia solanacearum are two of the most important pathogens affecting tobacco worldwide. greater insight regarding genetic systems controlling resistance to these two soilborne pathogens, as well as identification of dna markers associated with genomic regions controlling this resistance, could aid in variety development. an evaluation of 50 historical tobacco lines revealed a high positive correlation between resistances to the two pathogens, preliminarily suggest ... | 2017 | 28581342 |
| eggplant resistance to the ralstonia solanacearum species complex involves both broad-spectrum and strain-specific quantitative trait loci. | bacterial wilt (bw) is a major disease of solanaceous crops caused by the ralstonia solanacearum species complex (rssc). strains are grouped into five phylotypes (i, iia, iib, iii, and iv). varietal resistance is the most sustainable strategy for managing bw. nevertheless, breeding to improve cultivar resistance has been limited by the pathogen's extensive genetic diversity. identifying the genetic bases of specific and non-specific resistance is a prerequisite to breed improvement. a major gene ... | 2017 | 28580001 |
| ralstonia solanacearum and r. pseudosolanacearum on eucalyptus: opportunists or primary pathogens? | ralstonia solanacearum and r. pseudosolanacearum are well known primary pathogens of herbaceous crops. reports of wilt caused by these pathogens in tree species are limited other than on eucalyptus species. despite the widespread occurrence of so-called bacterial wilt on eucalypts in tropical and sub-tropical parts of africa, asia, and the americas, there remain many contradictions relating to the disease. our field observations over many years in most regions where the disease occurs on eucalyp ... | 2017 | 28553301 |
| recruitment of a lineage-specific virulence regulatory pathway promotes intracellular infection by a plant pathogen experimentally evolved into a legume symbiont. | ecological transitions between different lifestyles, such as pathogenicity, mutualism and saprophytism, have been very frequent in the course of microbial evolution, and often driven by horizontal gene transfer. yet, how genomes achieve the ecological transition initiated by the transfer of complex biological traits remains poorly known. here we used experimental evolution, genomics, transcriptomics and high-resolution phenotyping to analyze the evolution of the plant pathogen ralstonia solanace ... | 2017 | 28535261 |
| prokaryotic communities at different depths between soils with and without tomato bacterial wilt but pathogen-present in a single greenhouse. | the characterization of microbial communities that promote or suppress soil-borne pathogens is important for controlling plant diseases. we compared prokaryotic communities in soil with or without the signs of tomato bacterial wilt caused by ralstonia solanacearum. soil samples were collected from a greenhouse at two different depths because this pathogen is present in deep soil. we used samples from sites in which we detected phca, a key gene regulating r. solanacearum pathogenicity. the pyrose ... | 2017 | 28502968 |
| a practical guide to visualization and statistical analysis of r. solanacearum infection data using r. | this paper describes and summarizes approaches for visualization and statistical analysis using data from ralstonia solanacearum infection experiments based on methods and concepts that are broadly applicable. members of the r. solanacearum species complex cause bacterial wilt disease. bacterial wilt is a lethal plant disease and has been studied for over 100 years. during this time various methods to quantify disease and different ways to analyze the generated data have been employed. here, i a ... | 2017 | 28484483 |
| comparative analysis of ralstonia solanacearum methylomes. | ralstonia solanacearum is an important soil-borne plant pathogen with broad geographical distribution and the ability to cause wilt disease in many agriculturally important crops. genome sequencing of multiple r. solanacearum strains has identified both unique and shared genetic traits influencing their evolution and ability to colonize plant hosts. previous research has shown that dna methylation can drive speciation and modulate virulence in bacteria, but the impact of epigenetic modifications ... | 2017 | 28450872 |
| genome sequencing and analysis of ralstonia solanacearum phylotype i strains fjat-91, fjat-452 and fjat-462 isolated from tomato, eggplant, and chili pepper in china. | ralstonia solanacearum is an extremely destructive pathogen able to cause disease in a wide range of host plants. here we report the draft genome sequences of the strains fjat-91, fjat-452 and fjat-462, isolated from tomato, eggplant, and chili pepper, respectively, in china. in addition to the genome annotation, we performed a search for type-iii secreted effectors in these strains, providing a detailed annotation of their presence and distinctive features compared to the effector repertoire of ... | 2017 | 28428834 |
| volatile organic compounds produced by pseudomonas fluorescens wr-1 restrict the growth and virulence traits of ralstonia solanacearum. | the volatile organic compounds (vocs) produced by soil microbes have a significant role in the control of plant diseases and plant growth promotion. in this study, we examined the effect of vocs produced by pseudomonas fluorescens strain wr-1 on the growth and virulence traits of tomato wilt pathogen ralstonia solanacearum. the vocs produced by p. fluorescens wr-1 exhibited concentration dependent bacteriostatic effect on the growth of r. solanacearum on agar medium and in infested soil. the voc ... | 2016 | 27664728 |
| synthesis and antibacterial evaluation of new sulfone derivatives containing 2-aroxymethyl-1,3,4-oxadiazole/thiadiazole moiety. | sulfones are one of the most important classes of agricultural fungicides. to discover new lead compounds with high antibacterial activity, a series of new sulfone derivatives were designed and synthesized by introducing the aroxymethyl moiety into the scaffold of 1,3,4-oxadiazole/thiadiazole sulfones. antibacterial activities against three phytopathogens (xanthomonas oryzae pv. oryzae, ralstonia solanacearum, xanthomonas axonopodis pv. citri.) were assayed in vitro. as compared to the control o ... | 2016 | 28042864 |
| studies on the biosynthesis of ralfuranones in ralstonia solanacearum. | ralfuranones, aryl-furanone secondary metabolites, are involved in the virulence of ralstonia solanacearum in solanaceous plants. ralfuranone i (6) has been suggested as a biosynthetic precursor for other ralfuranones; however, this conversion has not been confirmed. we herein investigate the biosynthesis of ralfuranones using feeding experiments with ralfuranone i (6) and its putative metabolite, ralfuranone b (2). the results obtained demonstrated that the biosynthesis of ralfuranones proceede ... | 2016 | 26645956 |
| comparative secretome analysis of ralstonia solanacearum type 3 secretion-associated mutants reveals a fine control of effector delivery, essential for bacterial pathogenicity. | ralstonia solanacearum, the causal agent of bacterial wilt, exerts its pathogenicity through more than a hundred secreted proteins, many of them depending directly on the functionality of a type 3 secretion system. to date, only few type 3 effectors have been identified as required for bacterial pathogenicity, notably because of redundancy among the large r. solanacearum effector repertoire. in order to identify groups of effectors collectively promoting disease on susceptible hosts, we investig ... | 2016 | 26637540 |
| the vascular plant-pathogenic bacterium ralstonia solanacearum produces biofilms required for its virulence on the surfaces of tomato cells adjacent to intercellular spaces. | the mechanism of colonization of intercellular spaces by the soil-borne and vascular plant-pathogenic bacterium ralstonia solanacearum strain oe1-1 after invasion into host plants remains unclear. to analyse the behaviour of oe1-1 cells in intercellular spaces, tomato leaves with the lower epidermis layers excised after infiltration with oe1-1 were observed under a scanning electron microscope. oe1-1 cells formed microcolonies on the surfaces of tomato cells adjacent to intercellular spaces, and ... | 2016 | 26609568 |
| rapid differentiation of ralstonia solanacearum avirulent and virulent strains by cell fractioning of an isolate using high performance liquid chromatography. | ralstonia solanacearum is one of the most destructive plant bacterial pathogens worldwide. the population dynamics and genetic stability are important issues, especially when an avirulent strain is used for biocontrol. in this study, we developed a rapid method to differentiate the virulent and avirulent strains of r. solanacearum and to predict the biocontrol efficiency of an avirulent strain using high performance liquid chromatography (hplc). three chromatographic peaks p1, p2 and p3 were obs ... | 2016 | 26606869 |
| extract of syringa oblata: a new biocontrol agent against tobacco bacterial wilt caused by ralstonia solanacearum. | ralstonia solanacearum causes serious wilt disease in tobacco. to effectively control this disease, the antibacterial activity of 95% ethanol extracts from the flower buds of syringa oblata was examined. based on gc-ms analysis and an inhibition experiment against r. solanacearum, the main antibacterial component is eugenol. we further determined the effect of eugenol on the physiology, biochemistry, and cellular morphology of r. solanacearum. the results showed that eugenol can destroy wilt bac ... | 2016 | 27914543 |
| resveratrol and coumarin: novel agricultural antibacterial agent against ralstonia solanacearum in vitro and in vivo. | bacterial wilt is a destructive disease caused by the phytopathogen ralstonia solanacearum (r. solanacearum), which is widely found in various tobacco-growing areas all over the world. botanical bactericidal substances have gradually emerged as a hot topic in modern pesticide research. in this study, the antibacterial activities of two phytochemicals (resveratrol and coumarin) against r. solanacearum and their in vivo and in vitro efficacy for controlling tobacco bacterial wilt were evaluated. w ... | 2016 | 27834875 |
| extract from maize (zea mays l.): antibacterial activity of dimboa and its derivatives against ralstonia solanacearum. | many cereals accumulate hydroxamic acids involved in defense of plant against various fungi, bacteria, and insects. 2,4-dihydroxy-7-methoxy-1,4-benzoxazine-3-one, commonly known as dimboa, is one of the principal cyclic hydroxamic acids in aqueous extracts of maize. the aim of this study was to evaluate the antibacterial activity of the isolated dimboa and its derivatives 2-benzoxazolinone (boa), 6-chloro-2-benzoxazolinone (cdhb), and 2-mercaptobenzothiazole (mbt) against ralstonia solanacearum. ... | 2016 | 27775575 |
| the ralstonia solanacearum type iii effector ripay targets plant redox regulators to suppress immune responses. | the subversion of plant cellular functions is essential for bacterial pathogens to proliferate in host plants and cause disease. most bacterial plant pathogens employ a type iii secretion system to inject type iii effector (t3e) proteins inside plant cells, where they contribute to the pathogen-induced alteration of plant physiology. in this work, we found that the ralstonia solanacearum t3e ripay suppresses plant immune responses triggered by bacterial elicitors and by the phytohormone salicyli ... | 2016 | 27768829 |
| the phytopathogenic virulent effector protein ripi induces apoptosis in budding yeast saccharomyces cerevisiae. | virulent protein toxins secreted by the bacterial pathogens can cause cytotoxicity by various molecular mechanisms to combat host cell defense. on the other hand, these proteins can also be used as probes to investigate the defense pathway of host innate immunity. ralstonia solanacearum, one of the most virulent bacterial phytopathogens, translocates more than 70 effector proteins via type iii secretion system during infection. here, we characterized the cytotoxicity of effector ripi in budding ... | 2016 | 27616453 |
| three new sesquiterpenoids from agarwood of aquilaria crassna. | three new sesquiterpenoids (1-3), together with two known ones were isolated from the etoac extract of agarwood originating from aquilaria crassna. the new compounds were elucidated on the basis of spectroscopic techniques (uv, ir, ms, 1d and 2d nmr). compounds 1-5 were isolated from agarwood of a. crassna for the first time. in the acetylcholinesterase inhibition experiment of 2-5, compound 3 showed acetylcholinesterase inhibition activity (ir 42.9±0.6%). compound 5 expressed antibacterial acti ... | 2016 | 27502285 |
| a novel leucine-rich repeat protein, calrr51, acts as a positive regulator in the response of pepper to ralstonia solanacearum infection. | the leucine-rich repeat (lrr) proteins play important roles in the recognition of corresponding ligands and signal transduction networks in plant defence responses. herein, a novel lrr protein from capsicum annuum, calrr51, was identified and characterized. it was localized to the plasma membrane and transcriptionally up-regulated by ralstonia solanacearum infection (rsi), as well as the exogenous application of salicylic acid (sa), jasmonic acid (ja) and ethephon (eth). virus-induced gene silen ... | 2016 | 27438958 |
| development and comparison of taqman-based real-time pcr assays for detection and differentiation of ralstonia solanacearum strains. | bacterial wilt caused by ralstonia solanacearum is destructive to many plant species worldwide. the race 3 biovar 2 (r3b2) strains of r. solanacearum infect potatoes in temperate climates and are listed as select agents by the u.s. government. taqman-based real-time quantitative pcr (qpcr) is commonly used in federal and state diagnostic laboratories over conventional pcr due to its speed and sensitivity. we developed the rs16s primers and probe set and compared it with a widely used set (rs) fo ... | 2016 | 27402488 |
| extracellular dnases of ralstonia solanacearum modulate biofilms and facilitate bacterial wilt virulence. | ralstonia solanacearum is a soil-borne vascular pathogen that colonizes plant xylem vessels, a flowing, low-nutrient habitat where biofilms could be adaptive. ralstonia solanacearum forms biofilm in vitro, but it was not known if the pathogen benefits from biofilms during infection. scanning electron microscopy revealed that during tomato infection, r. solanacearum forms biofilm-like masses in xylem vessels. these aggregates contain bacteria embedded in a matrix including chromatin-like fibres c ... | 2016 | 27387368 |
| screening oligosaccharide libraries against lectins using the proxy protein electrospray ionization mass spectrometry assay. | an electrospray ionization mass spectrometry (esi-ms) assay for screening carbohydrate libraries against lectins is described. the assay is based on the proxy protein esi-ms method, which combines direct esi-ms protein-ligand binding measurements and competitive protein binding, to simultaneously detect and quantify protein-carbohydrate interactions. specific interactions between components of the library and the target protein (pt) are identified from changes in the relative abundances (as meas ... | 2016 | 27366913 |
| evaluation of the antibacterial effects and mechanism of action of protocatechualdehyde against ralstonia solanacearum. | protocatechualdehyde (pca) is an important plant-derived natural product that has been associated with a wide variety of biological activities and has been widely used in medicine as an antioxidant, anti-aging and an anti-inflammatory agent. however, fewer reports concerning its antibacterial effects on plant-pathogenic bacteria exist. therefore, in this study, protocatechualdehyde was evaluated for its antibacterial activity against plant pathogens along with the mechanism of its antibacterial ... | 2016 | 27294898 |
| root border cells and their role in plant defense. | root border cells separate from plant root tips and disperse into the soil environment. in most species, each root tip can produce thousands of metabolically active cells daily, with specialized patterns of gene expression. their function has been an enduring mystery. recent studies suggest that border cells operate in a manner similar to mammalian neutrophils: both cell types export a complex of extracellular dna (exdna) and antimicrobial proteins that neutralize threats by trapping pathogens a ... | 2016 | 27215971 |
| the hidden conformation of lewis x, a human histo-blood group antigen, is a determinant for recognition by pathogen lectins. | histo-blood group epitopes are fucosylated branched oligosaccharides with well-defined conformations in solution that are recognized by receptors, such as lectins from pathogens. we report here the results of a series of experimental and computational endeavors revealing the unusual distortion of histo-blood group antigens by bacterial and fungal lectins. the lewis x trisaccharide adopts a rigid closed conformation in solution, while crystallography and molecular dynamics reveal several higher e ... | 2016 | 27198630 |
| anaerobic digestion in mesophilic and room temperature conditions: digestion performance and soil-borne pathogen survival. | tomato plant waste (tpw) was used as the feedstock of a batch anaerobic reactor to evaluate the effect of anaerobic digestion on ralstonia solanacearum and phytophthora capsici survival. batch experiments were carried out for ts (total solid) concentrations of 2%, 4% and 6% respectively, at mesophilic (37±1°c) and room (20-25°c) temperatures. results showed that higher digestion performance was achieved under mesophilic digestion temperature and lower ts concentration conditions. the biogas prod ... | 2016 | 27155428 |
| two asian jumbo phages, ϕrsl2 and ϕrsf1, infect ralstonia solanacearum and show common features of ϕkz-related phages. | jumbo phages infecting ralstonia solanacearum were isolated in thailand (ϕrsl2) and japan (ϕrsf1). they were similar regarding virion morphology, genomic arrangement, and host range. phylogenetic and proteomic tree analyses demonstrate that the ϕrsl2 and ϕrsf1 belong to a group of evolutionary related phages, including pseudomonas phages ϕkz, 201ϕ2-1 and all previously described ϕkz-related phages. despite conserved genomic co-linearity between the ϕrsl2 and ϕrsf1, they differ in protein separat ... | 2016 | 27081857 |
| new insights into the antibacterial activity of hydroxycoumarins against ralstonia solanacearum. | coumarins are important plant-derived natural products with wide-ranging bioactivities and extensive applications. in this study, we evaluated for the first time the antibacterial activity and mechanisms of action of coumarins against the phytopathogen ralstonia solanacearum, and investigated the effect of functional group substitution. we first tested the antibacterial activity of 18 plant-derived coumarins with different substitution patterns, and found that daphnetin, esculetin, xanthotol, an ... | 2016 | 27070570 |
| overexpression of a harpin-encoding gene popw from ralstonia solanacearum primed antioxidant defenses with enhanced drought tolerance in tobacco plants. | the tobacco plants transformed with popw gene showed enhanced drought tolerance, and the mechanism was found with primed antioxidant defenses and reduced drought stress damages in the transgenic lines. harpin proteins are elicitors produced by several gram-negative plant pathogenic bacteria, triggering multiple beneficial responses in plants, such as induction of defense response against diverse pathogens and insects, growth promotion, and drought tolerance. in this study, the harpin-encoding ge ... | 2016 | 27053225 |
| enzymatic modification of chitosan by cinnamic acids: antibacterial activity against ralstonia solanacearum. | this study aimed to identify chitosan polymers that have antibacterial activity against the bacterial wilt pathogen. the chitosan polymers were enzymatically synthesized using chitosan and five cinnamic acids (cads): caffeic acid (ca), ferulic acid (fa), cinnamic acid (cia), p-coumaric acid (coa) and chlorogenic acid (cha), using laccase from pleurotus ostreatus as a catalyst. the reaction was performed in a phosphate buffered solution under heterogenous reaction conditions. the chitosan derivat ... | 2016 | 26993531 |