Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| purification and characterization of helicobacter mustelae urease. | helicobacter mustelae is a urease-rich bacterium associated with gastritis in ferrets. the ureases of h. mustelae and helicobacter pylori, a bacterium implicated in human gastritis, share many characteristics. helicobacter sp. ureases appear to be unique among bacterial enzymes in exhibiting submillimolar km values and in being composed of two subunits. | 1991 | 1879950 |
| animal models of bacterial gastritis: the role of host, bacterial species and duration of infection on severity of gastritis. | gastric bacteria from cheetahs with gastritis were used to inoculate specific-pathogen free kittens and conventional mice. helicobacter sp. and gastrospirillum sp. colonized kittens, while only gastrospirillum sp. colonized mice. in kittens, both bacterial species induced mild lymphofolliclar gastritis which did not change over the course of the 11 months observation period. in mice, gastrospirillum sp. induced lymphoplasmacytic and follicular gastritis which increased in severity over 6 months ... | 1993 | 8280953 |
| epizootic gastritis associated with gastric spiral bacilli in cheetahs (acinonyx jubatus). | an outbreak of vomiting in a group of captive cheetahs (acinonyx jubatus) was investigated, and histologic examination revealed chronic gastritis characterized by infiltration of lymphocytes and numerous plasma cells and epithelial erosions. lymphoid follicles, globule leukocytes, scattered neutrophils, and (in one animal) abscessed gastric glands were inconsistent findings. in addition, necropsy of three cheetahs revealed gastric mucosal hyperplasia. two kinds of bacteria were identified in the ... | 1993 | 8442328 |
| phylogeny of helicobacter isolates from bird and swine feces and description of helicobacter pametensis sp. nov. | previously, nine fecal isolates from wild birds and a domestic swine were identified as helicobacters by phenotypic characterization and reaction with a helicobacter genus-specific dna probe. these isolates fell into three biotypes by analysis of phenotypic traits. to further characterize these isolates, full 16s rrna sequences were determined for strains representing each biotype, and sequence comparison indicated that the strains represented three novel, phylogenetically defined helicobacter s ... | 1994 | 7520743 |
| the use of a mouse model in the study of helicobacter sp.-associated gastric cancer. | hypothesis: long-term infection with helicobacter pylori and the associated gastritis is now thought to cause a predisposition to gastric cancer through cellular changes resulting from inflammatory damage or because of direct effects of the bacterium. mice as models for h. pylori-associated gastric cancer: long-term infection of conventional swiss mice with either h. felis or h. heilmannii results in atrophic gastritis. infection of specific pathogen-free balb/c mice results in the development o ... | 1994 | 7735939 |
| helicobacter sp. strain mainz isolated from an aids patient with septic arthritis: case report and nonradioactive analysis of 16s rrna sequence. | a campylobacter-like organism was isolated from an effusion of the left knee joint of an aids patient 2 weeks after bacteremia with a morphologically identical organism. amplified genomic 16s rrna sequences were analyzed by a nonradioactive blotting technique. the closest match was found with helicobacter fenelliae (97.7% homology). sequence data and phenotype suggest that the isolate may represent a so far unrecognized species of the genus helicobacter. | 1994 | 7883895 |
| recombinant antigens prepared from the urease subunits of helicobacter spp.: evidence of protection in a mouse model of gastric infection. | urease is an important virulence factor for gastric helicobacter spp. to elucidate the efficacy of individual urease subunits to act as mucosal immunogens, the genes encoding the respective urease subunits (urea and ureb) of helicobacter pylori and helicobacter felis were cloned in an expression vector (pmal) and expressed in escherichia coli cells as translational fusion proteins. the recombinant urea and ureb proteins were purified by affinity and anion-exchange chromatography techniques and h ... | 1994 | 7927778 |
| characterization of cilia-associated respiratory bacillus in rabbits and analysis of the 16s rrna gene sequence. | the cilia-associated respiratory (car) bacillus is an unclassified, gram-negative bacterium that has been implicated as an etiologic agent of respiratory tract disease in laboratory rodents. a morphologically and antigenically similar organism has been identified in rabbits and is thought to be a related bacterium, although clinical signs of disease and histologic lesions are absent in infected rabbits. to compare the pathogenicity of rat- and rabbit-origin car bacillus isolates in rabbits, neon ... | 1995 | 7538614 |
| culture and characteristics of helicobacter bizzozeronii, a new canine gastric helicobacter sp. | organisms whose cells were large, tight spirals were isolated from gastric biopsies of dogs. touch cytology samples from all of the dogs contained large spiral organisms. characteristics of 10 strains are described. these organisms were 5 to 10 microns long by 0.3 microns wide, and each cell had 10 to 20 sheathed flagella at both ends of the cell. the cells did not have periplasmic fibrils. these organisms were microaerophilic and grew at 37 and 42 degrees c but not at 25 degrees c on brain hear ... | 1996 | 8573490 |
| lipid profile of helicobacter spp.: presence of cholesteryl glucoside as a characteristic feature. | the lipid and fatty acid profiles of eight helicobacter spp. (h. nemestrinae, h. acinonyx, h. canis, helicobacter sp. strain clo-3, "h. rappini" [flexispira rappini], h. pametensis, helicobacter sp. strain bird-b, and helicobacter sp. strain bird-c) and the fatty acid profiles of five additional species (h. pylori, h. felis, h. muridarum, h. mustelae, and h. fennelliae) were analyzed and compared. a heterologous fatty acid profile was observed among the helicobacter spp., and on that basis the s ... | 1996 | 8606185 |
| production of a monoclonal antibody against the 128 kda (caga) protein of helicobacter pylori. | helicobacter pylori is recognised as an important factor in gastroduodenal pathology. the 128 kda caga protein has been established as a useful marker of h. pylori strains associated with more severe forms of disease. a mouse monoclonal antibody raised against the caga protein has been produced and characterised as belonging to the igg1 subtype. it identified the protein in all clinical isolates (10/10) from this laboratory and in two nctc reference strains (nctc 11637 and nctc 11961). no cross- ... | 1996 | 8621951 |
| hemorrhagic gastritis in free-living rodents in idaho. | between february 1992 and march 1994, four species of rodent from the snake river birds of prey area near boise, idaho (usa) were necropsied. hemorrhagic gastritis was observed in 16 of 131 townsend's ground squirrels (spermophilus townsendii), one of 11 ord's kangaroo rats (dipodomys ordii) and the one great basin pocket mouse (perognathus parvus) evaluated. no lesions were observed in 14 white-footed deer mice (peromyscus maniculatus). tissue from one townsend's ground squirrel was negative fo ... | 1996 | 9359067 |
| helicobacter mustelae-associated gastric adenocarcinoma in ferrets (mustela putorius furo). | helicobacter pylori in humans is associated with active, chronic gastritis, peptic ulcer disease, and most recently has been linked epidemiologically to gastric adenocarcinoma. a related organism, helicobacter mustelae, naturally infects ferrets and also causes a persistent gastritis, a precancerous lesion, and focal glandular atrophy of the proximal antrum. in this report, we document the clinical presentation and histopathologic confirmation of h. mustelae-associated gastric adenocarcinoma in ... | 1997 | 9163879 |
| pathogenic role of gastric helicobacter sp in domestic carnivores. | as a result of phylogenic studies using new molecular biology techniques and fundamental experimental studies, we now know more about helicobacteria in domestic carnivores, their morphologic characteristics, their taxonomia and more important we know more about their ecological niche. few clinical studies have been carried out, but the ones that have been undertaken are interesting in that they confirm the extensive prevalence of helicobacter infections in domestic carnivores and underline their ... | 1997 | 9208441 |
| helicobacter salomonis sp. nov., a canine gastric helicobacter sp. related to helicobacter felis and helicobacter bizzozeronii. | during a study of the prevalence and distribution of gastric helicobacters in domestic pets, a novel group of helicobacter-like organisms were identified. these "helicobacter group 2" strains were initially distinguished from the species helicobacter felis and helicobacter bizzozeronii by their cellular morphology and the type of motility exhibited. bacterial cells were only slightly spiral, 5 to 7 microns long, and 0.8 to 1.2 microns wide and showed an unusual slow wavelike motion. each cell ha ... | 1997 | 9336895 |
| effect of potent urease inhibitor, fluorofamide, on helicobacter sp. in vivo and in vitro. | the therapeutic potential of urease inhibition of helicobacter pylori has been studied by examining the effect of the potent urease inhibitor, fluorofamide (n-(diaminophosphinyl)-4-fluorobenzenamide), on urease activity and bacterial survival in vivo and in vitro. in culture, acid protection in h. pylori was shown to be due to changes in the ph of the medium brought about by the release of ammonia. both the acid protection and the ammonia release were completely blocked by fluorofamide at low do ... | 1998 | 9508511 |
| hepatic helicobacter species identified in bile and gallbladder tissue from chileans with chronic cholecystitis. | cancer of the gallbladder is the number one cause of cancer mortality in chilean women. incidence rates for this tumor vary widely on a worldwide basis, being approximately 30 times higher in high-risk than in low-risk populations, suggesting that environmental factors such as infectious microorganisms, carcinogens, and nutrition play a role in its pathogenesis. because several helicobacter sp. colonize the livers of animals and induce hepatitis, the aim of this study was to determine whether he ... | 1998 | 9516396 |
| identification of a novel enteric helicobacter species in a kitten with severe diarrhea. | a previously undescribed helicobacter sp. was recovered from a cat with severe diarrhea. based upon the absence of any other identifiable cause of diarrhea, this helicobacter may be involved in the development of the disease signs. the organism could not be cultured but was described on the basis of 16s rrna gene sequence analysis and morphology and appeared to be a new species, with helicobacter canis being the most genetically similar species. the presence of a diarrhea-inducing helicobacter i ... | 1998 | 9542907 |
| green fluorescent protein as a novel marker and reporter system in helicobacter sp. | in order to be able to study gene regulation in single, live helicobacter pylori bacteria in vitro or in contact with host cells, we established the green fluorescent protein gene gfp from aequorea victoria as a reporter gene for use with helicobacter species. we describe here the construction of genomic transcriptional fusions of the promoterless gfp gene with the flaa and flab promoters of h. pylori. we have also constructed a mini-tn3-km-gfp transposon to be used for shuttle transposon mutage ... | 1998 | 9570118 |
| novel intestinal helicobacter species isolated from cotton-top tamarins (saguinus oedipus) with chronic colitis. | a disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (ctts) in captivity. the clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. approximately 25 to 40% of these animals develop colon cancer after 2 to 5 years of captivity. an infectious etiology has been proposed; however, no microbial agent to date has been identified. helicobacter spp. have been associated ... | 1999 | 9854080 |
| characterization of a culturable "gastrospirillum hominis" (helicobacter heilmannii) strain isolated from human gastric mucosa. | spiral organisms were isolated from an antral gastric mucosal biopsy specimen from a dyspeptic patient with gastritis. only corkscrew-shaped organisms resembling "gastrospirillum hominis" ("helicobacter heilmannii") but no helicobacter pylori-like organisms were seen in histological sections. h. pylori was not cultured from specimens from this patient. on the basis of biochemical reactions, morphology, ultrastructure, and 16s dna sequencing, the isolated "g. hominis" was shown to be a true helic ... | 1999 | 10074528 |
| a novel urease-negative helicobacter species associated with colitis and typhlitis in il-10-deficient mice. | a spiral-shaped bacterium with bipolar, single-sheathed flagella was isolated from the intestines of il-10 (interleukin-10)-deficient (il-10(-/-)) mice with inflammatory bowel disease. the organism was microaerobic, grew at 37 and 42 degrees c, and was oxidase and catalase positive but urease negative. on the basis of 16s rrna gene sequence analysis and biochemical and phenotypic criteria, the organism is classified as a novel helicobacter. cesarean section-rederived il-10(-/-) mice without heli ... | 1999 | 10085015 |
| helicobacter sp. are not detectable in bile from german patients with biliary disease. | 1999 | 10092334 | |
| pathology, diagnosis and epidemiology of the rodent helicobacter infection. | since the first isolation of helicobacter pylori from humans in 1983, 18 helicobacter species have been identified during the last decade in domestic and laboratory animals. several helicobacter species have been isolated from the gastrointestinal tracts of various mammalian species and birds. helicobacter hepaticus, h. muridarum, h. bilis, h. rodentium and flexispira rappini have been isolated from mice. among these species, only h. hepaticos has been clearly recognized as a pathogen. indeed, i ... | 1999 | 10099028 |
| an uncommon helicobacter isolate from blood: evidence of a group of helicobacter spp. pathogenic in aids patients. | an unusual helicobacter sp. was isolated from the blood of a human immunodeficiency virus (hiv)-infected patient. this organism had spiral morphology, with single amphitrichous flagella, and was negative for hippurate hydrolysis, production of urease, and reduction of nitrate. 16s rrna gene sequence analysis verified that the isolate was a species of helicobacter, most closely related to an undescribed helicobacter-like isolate from vancouver, british columbia, canada, and to helicobacter westme ... | 1999 | 10405434 |
| colonization and tissue tropism of helicobacter pylori and a novel urease-negative helicobacter species in icr mice are independent of route of exposure. | in humans, helicobacter pylori is known to colonize the stomach and to induce persistent gastritis; selected reports also suggest it causes extragastric disease, including hepatitis. h. pylori and a novel urease-negative helicobacter sp. induce gastritis and typhlocolitis, respectively, when inoculated orally into mice. experimental typhlocolitis and hepatitis have been caused by intraperitoneal (i.p.) injection of h. hepaticus, h. bilis, and the novel helicobacter spp. however, the route by whi ... | 1999 | 10597395 |
| porcine gastric ulcer. | ulceration of the pars esophagea in swine develops from a complex interaction of dietary particle size, gastric fluidity, dietary carbohydrate content, and presence of certain species of commensal gastric organisms capable of fermenting dietary carbohydrates. unlike in humans, the significance of the role of helicobacter sp. in development of porcine gastric ulcers is yet undefined. management practices that limit the incidence and severity of gastric ulceration without interfering with growth p ... | 2000 | 10707418 |
| evidence that colitis is initiated by environmental stress and sustained by fecal factors in the cotton-top tamarin (saguinus oedipus). | parallel changes in spontaneously occurring inflammation in colonic thiry-vella loops and the in-line colon of cotton-top tamarins were studied in a colitis-inducing environment at 8 and 15 months following surgical preparation of the loops. gross disease severity and numbers of inflammatory/immune cells per unit area of lamina propria in histological sections from endoscopic biopsies were analyzed. cell counts and severity of colitis declined over time in the thiry-villa loops while the disease ... | 2000 | 10711456 |
| misidentifying helicobacters: the helicobacter cinaedi example. | whole-cell protein electrophoresis and biochemical examination by means of a panel of 64 tests were used to identify 14 putative helicobacters to the species level. the results were confirmed by means of dna-dna hybridization experiments and were used to discuss misidentification of helicobacters based on 16s rrna gene sequence data. the data indicated that comparison of near-complete 16s ribosomal dna sequences does not always provide conclusive evidence for species level identification and may ... | 2000 | 10834986 |
| identification of a unique helicobacter species by 16s rrna gene analysis in an abdominal abscess from a patient with x-linked hypogammaglobulinemia. | a unique helicobacter species, mz640285, was isolated from a patient with x-linked hypogammaglobulinemia suffering from recurrent abdominal abscesses and was identified by 16s rrna gene sequence analysis. in the phylogenetic tree, the isolate fell into a cluster which included flexispira rappini, helicobacter bilis, and helicobacter sp. strain mainz. helicobacters are being increasingly recognized as pathogens in immunocompromised hosts. these fastidious bacteria are not easily cultured in the r ... | 2000 | 10878075 |
| identification of enterohepatic helicobacter species by restriction fragment-length polymorphism analysis of the 16s rrna gene. | restriction fragment-length polymorphism (rflp) analysis of a 1,200-bp polymerase chain reaction-amplified dna fragment of gene coding for 16s rrna was used to generate restriction profiles of 11 enterohepatic helicobacter spp. isolated from various animals and humans. | 2000 | 10971675 |
| helicobacter aurati sp. nov., a urease-positive helicobacter species cultured from gastrointestinal tissues of syrian hamsters. | a novel helicobacter with the proposed name helicobacter aurati (type strain mit 97-5075c) has been isolated from the inflamed stomachs and ceca of adult syrian hamsters. the new species is fusiform with multiple bipolar sheathed flagella and periplasmic fibers; it contains urease and gamma-glutamyl transpeptidase. by 16s rrna sequencing and repetitive element pcr-based dna fingerprinting, it was found that h. aurati represents a distinct taxon and clusters with helicobacter muridarum, helicobac ... | 2000 | 11015391 |
| isolation and characterization of a helicobacter sp. from the gastric mucosa of dolphins, lagenorhynchus acutus and delphinus delphis. | gastric ulcerations in dolphins have been reported for decades. some of these lesions were associated with parasitic infections. however, cases of nonparasitic gastric ulcers with no clearly defined etiology also have been reported in wild and captive dolphins. considerable speculation exists as to whether dolphins have helicobacter-associated gastritis and peptic ulcer disease. the stomachs of seven stranded atlantic white-sided dolphins, lagenorhynchus acutus, and 1 common dolphin, delphinus d ... | 2000 | 11055919 |
| gastritis and intestinal metaplasia in syrian hamsters infected with helicobacter aurati and two other microaerobes. | chronic gastritis and intestinal metaplasia associated with naturally occurring colonization by helicobacter aurati and two other microaerobic species were observed in syrian hamsters. thirty-five hamsters, between 7 and 12 months of age, were evaluated from two research and three commercial facilities. microaerobic bacteria were cultured from the hamster stomachs. these bacteria included h. aurati, a fusiform, urease-positive species; a second novel helical, urease-negative helicobacter sp.; as ... | 2000 | 11105948 |
| lack of association between helicobacter sp colonization and gallstone disease. | recently, helicobacter sp has been identified in resected gallbladder tissue and in collected bile from chilean patients with chronic cholecystitis. therefore, it an association between bile helicobacter sp and gallbladder cancer has been proposed. interestingly, both helicobacter colonization and gallstone disease (gd) happen very frequently in chile. however, whether there is an association between helicobacter colonization and gd has not been completely studied. the aim of this study was to d ... | 2001 | 11205649 |
| helicobacter sp. recovered from drinking water biofilm sampled from a water distribution system. | workers examining the transmission route(s) and reservoir(s) of infection for helicobacter pylori have postulated several environmental reservoirs for the organism, including water. such work has, to date, concentrated on the bulk liquid in drinking water systems rather than on biofilms. previous investigations by the authors have suggested biofilms in water distribution systems are a possible reservoir of infection. this current study comprised of an analysis of a section of cast iron mains dis ... | 2001 | 11317912 |
| helicobacter sp. flexispira bacteremia in an immunocompetent young adult. | a young immunocompetent patient was admitted for a febrile illness with malaise, arthralgias, painful leg swelling, and polyserositis. shortly prior to becoming ill, the patient had traveled to the northern african desert. the symptoms disappeared during treatment with antibiotics (doxycycline and ceftriaxone) but recurred twice after stopping therapy. a motile gram-negative fusiform rod was isolated from a blood culture taken on the first admission. analysis of the 16s rrna gene of the blood cu ... | 2001 | 11325980 |
| helicobacter-induced expression of bcl-x(l) in b lymphocytes in the mouse model: a possible step in the development of gastric mucosa-associated lymphoid tissue (malt) lymphoma. | primary gastric mucosa-associated lymphoid tissue (malt) lymphoma may develop from chronic infection with helicobacter sp. in the mouse model. the mechanisms of pathogenesis remain unclear. regulation of b-cell proliferation and death are important features to investigate. proteins encoded by bcl-2 family genes, e.g., bcl-x(l), regulate apoptosis; and alterations in the expression of these genes can contribute to the development of cancer. our aim was to determine the role of bcl-x(l) in b lymph ... | 2001 | 11340565 |
| coinfection of enteric helicobacter spp. and campylobacter spp. in cats. | during a 6-year period, 64 of 227 commercially reared cats had microaerobic bacteria isolated from their feces. all the isolates were initially identified as campylobacter-like organisms based on biochemical and phenotypic characteristics. dna extractions from 51 of these isolates were subjected to pcr using primers specific for helicobacter spp. and campylobacter spp. of the isolates, 92% (47 of 51 isolates) were positive for campylobacter spp., 41% (21 of 51 isolates) were positive for helicob ... | 2001 | 11376052 |
| cholangiohepatitis and inflammatory bowel disease induced by a novel urease-negative helicobacter species in a/j and tac:icr:hascidfrf mice. | helicobacter bilis and h. hepaticus, both urease-positive intestinal helicobacters of mice, have been shown experimentally to induce proliferative typhlocolitis in scid mice. we recently isolated a urease-negative helicobacter sp. (h. sp.) that also induced proliferative typhlocolitis in pilot studies in scid mice. to determine the pathogenic potential of h. sp. in immunocompromised and immunocompetent mice, 5-week old male a/j or tac:icr:ha(icr)-scidfrf mice were inoculated by intraperitoneal ( ... | 2001 | 11393169 |
| helicobacter winghamensis sp. nov., a novel helicobacter sp. isolated from patients with gastroenteritis. | from 1997 to 1999 seven isolates of campylobacter-like organisms from five patients that were exhibiting symptoms of gastroenteritis, including fever, stomach malaise, and diarrhea, were investigated. the organisms were isolated from stool samples and found to exhibit a diverse colony morphology; hence multiple isolates were submitted from one of the patients. all isolates were found to be identical. the organisms were catalase, urease, alkaline phosphatase, and nitrate negative but oxidase and ... | 2001 | 11427547 |
| identification of novel helicobacter species in pig stomachs by pcr and partial sequencing. | evidence of infection with helicobacter species in pig stomach was investigated by the use of a pcr with helicobacter genus-specific primers. forty pig stomachs, each of four different ulcer lesion grades, 0, 1, 2, and 3 in the pars esophagea area, were collected from a slaughterhouse in minnesota. of 160 stomach samples examined, 102 (63.8%) were positive by the pcr assay. the 40 samples each of lesion grades 0, 1, 2, and 3 showed 22.5, 52.5, 85.0, and 95.0% pcr-positive results, respectively. ... | 2001 | 11526168 |
| identification of helicobacter pylori dna in human cholesterol gallstones. | the gallbladder mucosa secretes hydrogen ions and is covered by mucus. the environmental conditions for bacterial colonization are similar to those in the stomach. gallbladder stones often contain dna from enteric bacteria, but no compelling evidence demonstrates that helicobacter spp. have been present. the aim of this study was to establish bacterial dna profiles in cholesterol gallstones with special reference to helicobacter pylori. | 2002 | 11843027 |
| hepatobiliary inflammation, neoplasia, and argyrophilic bacteria in a ferret colony. | hepatobiliary disease was diagnosed in eight of 34 genetically unrelated cohabitating pet ferrets (mustela putorios furo) during a 7-year period. the eight ferrets ranged in age from 5 to 8 years and exhibited chronic cholangiohepatitis coupled with cellular proliferation ranging from hyperplasia to frank neoplasia. spiral-shaped argyrophilic bacteria were demonstrated in livers of three ferrets, including two with carcinoma. sequence analysis of a 400-base pair polymerase chain reaction product ... | 2002 | 12009055 |
| isolation and characterization of campylobacter, helicobacter, and anaerobiospirillum strains from a puppy with bloody diarrhea. | we carried out a microscopic examination of stools from a 2-month-old female puppy with bloody diarrhea, and this revealed large numbers of different spiral-shaped bacteria. to isolate these organisms, a rectal swab specimen was inoculated onto plates of skirrow's agar and incubated at 37 degrees c for 6 days in a microaerobic atmosphere. finally, a total of six different spiral-shaped bacteria (strains g1104, 94105, fr106, b0101, 3j102, and j2103) were isolated. based on their morphology, bioch ... | 2002 | 12069772 |
| helicobacter marmotae sp. nov. isolated from livers of woodchucks and intestines of cats. | woodchucks (marmota monax) have a high incidence of hepatocellular carcinoma (hcc) associated with chronic infection with woodchuck hepatitis virus (whv) and serve as a model of hepatitis b virus-associated hcc in humans. helicobacter hepaticus, an enterohepatic helicobacter in mice, is known to cause hepatocellular adenomas and carcinomas in susceptible mouse strains. in long-term chemical bioassays conducted with b6c3f(1) mice, h. hepaticus has been regarded as a confounding factor because of ... | 2002 | 12089272 |
| unique case of helicobacter sp. osteomyelitis in an immunocompetent child diagnosed by broad-range 16s pcr. | we report the first case of helicobacter sp. osteomyelitis in an immunocompetent child. the infection was diagnosed by broad-range 16s pcr followed by sequencing of the resulting amplicon. all other microbiological investigations proved negative. this case highlights the importance of molecular methods in the diagnosis of unsuspected etiological agents and the potential role of helicobacter sp. in human infection. | 2002 | 12149392 |
| evidence of helicobacter sp. in dental plaque of captive dolphins (tursiops gephyreus). | gastrointestinal lesions have been extensively reported in wild and captive marine mammals. however, their etiology remains unclear. in humans and other animals, chronic gastritis and peptic ulcers have been associated with helicobacter sp. therefore, the aim of our study was to investigate the presence of helicobacter sp. in the gastric juice, dental plaque, and saliva of marine mammals living in a controlled environment. five dolphins (tursiops gephyreus), one killer whale (orcinus orca), one ... | 2002 | 12238390 |
| evaluation of direct fluorescent antibody test for the diagnosis of bovine genital campylobacteriosis. | the direct fluorescent antibody test (dfat) for the diagnosis of bovine genital campylobacteriosis was assessed for its detection limit, observer effect, sensitivity and specificity. in addition, the specificity of the fluorescent conjugate was tested against campylobacter sp, arcobacter sp, helicobacter sp, e. coli and other bacteria from the preputial flora. ten - fold dilutions of c. fetus subsp. venerealis nctc 10354 in pbs or preputial washings with or without centrifugation were used. all ... | 2002 | 17063594 |
| cytolethal distending toxin b gene (cdtb) homologues in taxa 2, 3 and 8 and in six canine isolates of helicobacter sp. flexispira. | the presence of the cytolethal distending toxin b gene (cdtb) was examined in eight helicobacter sp. flexispira reference strains, helicobacter trogontum atcc 700114(t) and 12 finnish porcine h. trogontum strains and canine flexispira isolates. part of the cdtb gene was amplified by pcr with degenerate primers vat2 and dhf1, cloned and sequenced. the presence/absence of the cdtb gene as determined by pcr was confirmed by southern hybridization and toxin production by hela cell-line experiments. ... | 2003 | 12543914 |
| the rapid detection of low molecular mass proteins differentially expressed under biological stress for four helicobacter spp. using proteinchip technology. | helicobacter pylori is one of the most prevalent human pathogens in the world and is the aetiological agent of gastritis, peptic ulcer disease and gastric malignancies. in addition h. pylori and other novel members of the genus are capable of successfully colonising the bile-rich niche of the upper intestine and are associated with a diverse range of intestinal pathologies. surface-enhanced laser desorption/ionisation-time of flight mass spectrometry was used to analyse surface extracts from h. ... | 2003 | 12627380 |
| helicobacter sp. flexispira 16s rdna taxa 1, 4 and 5 and finnish porcine helicobacter isolates are members of the species helicobacter trogontum (taxon 6). | the term 'flexispira' refers to micro-organisms with a particular morphology: fusiform-shaped with helical periplasmic fibrils and bipolar tufts of sheathed flagella. two flexispira taxa have been formally named, helicobacter bilis and helicobacter trogontum, a third named species is helicobacter aurati and eight additional 16s rrna sequence-based flexispira taxa have been described by dewhirst et al. (int j syst evol microbiol 50, 1781-1787, 2000) and given the provisional designation helicobac ... | 2003 | 12710608 |
| potential for proteomic profiling of helicobacter pylori and other helicobacter spp. using a proteinchip array. | the helicobacter genus is associated with a wide spectrum of pathologies in the gastrointestinal tract. however, in contrast to helicobacter pylori, few data are available regarding proteomic characteristics of enterohepatic helicobacters. proteomic analysis of this genus has predominantly utilised two-dimensional gel electrophoresis methodology. in the present study we applied an innovative technique using proteinchip arrays coupled with surface-enhanced laser desorption/ionisation time of flig ... | 2003 | 12738385 |
| pathogenesis of mouse hepatitis virus infection in gamma interferon-deficient mice is modulated by co-infection with helicobacter hepaticus. | gamma interferon-deficient (ifn-gamma ko) mice developed a wasting syndrome and were found to be co-infected with helicobacter sp., and a new isolate of mouse hepatitis virus (mhv) designated mhv-g. the disease was characterized by pleuritis, peritonitis, hepatitis, pneumonia, and meningitis. initial experiments used a cecal homogenate inoculum from the clinical cases that contained h. hepaticus and mhv-g to reproduce the development of peritonitis and pleuritis in ifn-gamma ko mice. in contrast ... | 2003 | 12784855 |
| comparison of diagnostic techniques for helicobacter cetorum infection in wild atlantic bottlenose dolphins (tursiops truncatus). | helicobacter cetorum sp. nov. has been cultured from the stomach of atlantic white-sided dolphins (lagenorhynchus acutus) and the feces of pacific white-sided (l. obliquidens) and atlantic bottlenose (tursiops truncatus) dolphins and a beluga whale (delphinapterus leucas). h. cetorum has high homology to helicobacter pylori as shown by 16s rrna sequencing, and h. cetorum infection has been associated with gastritis and clinical signs in cetaceans. because the prevalence of h. cetorum in wild pop ... | 2003 | 12843010 |
| the presence of helicobacter-like microorganisms in the gastric mucosa in dogs. | the aim of this study was the evaluation of the occurrence of helicobacter-like organisms in the gastric mucosa of dogs. the study was performed on samples of gastric mucosa obtained during necropsies from 94 dogs euthanasized for various reasons. histopathological examination and also urease test was performed. helicobacter sp. was found in 85.10% of examined dogs. in 36% cases the presence of bacteria was accompanied by chronic inflammation of gastric mucosa localized mainly in pyloric region. ... | 2003 | 14703868 |
| comparative chemical and biological characterization of the lipopolysaccharides of gastric and enterohepatic helicobacters. | the lipopolysaccharide of helicobacter pylori plays an important role in colonization and pathogenicity. the present study sought to compare structural and biological features of lipopolysaccharides from gastric and enterohepatic helicobacter spp. not previously characterized. | 2004 | 15270745 |
| high prevalence of helicobacter species detected in laboratory mouse strains by multiplex pcr-denaturing gradient gel electrophoresis and pyrosequencing. | rodent models have been developed to study the pathogenesis of diseases caused by helicobacter pylori, as well as by other gastric and intestinal helicobacter spp., but some murine enteric helicobacter spp. cause hepatobiliary and intestinal tract diseases in specific inbred strains of laboratory mice. to identify these murine helicobacter spp., we developed an assay based on pcr-denaturing gradient gel electrophoresis and pyrosequencing. nine strains of mice, maintained in four conventional lab ... | 2004 | 15297530 |
| validation of the 13c-urea breath test for use in cheetahs (acinonyx jubatus) with helicobacter. | historically, therapeutic monitoring for prescribed eradication treatment of helicobacter in cheetahs (acinonyx jubatus) with associated gastritis has been accomplished only through endoscopic biopsies. the 13c-urea breath test (ubt) can offer an alternative to repeated biopsies for therapeutic monitoring. five male and five female cheetahs and one male sumatran tiger (panthera tigris) were studied. all were clinically healthy before and after this investigation. breath samples of end-tidal expi ... | 2004 | 15305506 |
| detection of helicobacter ganmani-like 16s rdna in pediatric liver tissue. | to determine the presence of helicobacter species in the liver biopsy specimens from children with various chronic liver diseases as data in adult literature suggests a possible role of these bacteria in their pathogenesis. | 2004 | 15361086 |
| the high prevalence of helicobacter sp. in porcine pyloric mucosa and its histopathological and molecular characteristics. | this study examined the prevalence of helicobacter infection in the pyloric mucosa of pigs and its histopathological and molecular characteristics. forty porcine pyloric samples were examined for helicobacter infection by silver staining and pcr assay. the pcr product (376 bp) was digested with ndeii to differentiate between helicobacter heilmannii and helicobacter pylori. another pcr assay run to produce an 1157 bp fragment was performed using a primer set designed from the 16s rrna gene of can ... | 2004 | 15564031 |
| simple duplex fecal pcr assay that allows identification of false-negative results in helicobacter sp.-infected mice. | we designed a simple and sensitive duplex polymerase chain reaction (pcr) assay for detection of false-negative results during routine helicobacter sp. feces analysis. we took advantage of the various lactobacillus species that form part of the normal intestinal flora of laboratory rodents to improve our pcr diagnostic assays. using this one-step pcr assay, we were able to rule out false-negative results without the need of adding internal standard molecules. this is an important quality control ... | 2004 | 15575366 |
| extension of the species helicobacter bilis to include the reference strains of helicobacter sp. flexispira taxa 2, 3 and 8 and finnish canine and feline flexispira strains. | the evolution and taxonomy of enterohepatic helicobacter species with flexispira morphology were studied by a polyphasic approach including phenotypic characterization, analysis of 16s rrna and ureb gene sequences and dot-blot dna-dna hybridization of whole genomic dna. in addition, available phylogenetic data on the hsp60 gene were used in the analysis. the study included 14 finnish canine and feline flexispira strains, the reference strains of helicobacter sp. flexispira taxa 2, 3 and 8 and he ... | 2005 | 15774681 |
| dual infection with helicobacter bilis and helicobacter hepaticus in p-glycoprotein-deficient mdr1a-/- mice results in colitis that progresses to dysplasia. | patients with inflammatory bowel disease (ibd) are at increased risk for developing high-grade dysplasia and colorectal cancer. animal ibd models that develop dysplasia and neoplasia may help elucidate the link between inflammation and colorectal cancer. mdr1a-/- mice lack the membrane efflux pump p-glycoprotein and spontaneously develop ibd that can be modulated by infection with helicobacter sp: h. bilis accelerates development of colitis while h. hepaticus delays disease. in this study, we de ... | 2005 | 15920164 |
| identification of helicobacter sp. in gastric mucosa from captive marmosets (callithrix sp.; callitrichidae, primates). | the aim of this study was to identify the presence of helicobacter sp. in the gastric mucosa of captive marmosets (callithrix sp.). histologic specimens from the fundic, corpus, and antral gastric regions of six callithrix jacchus, 12 c. kuhli, and 12 c. geoffroyi specimens were evaluated. the sections were stained with hematoxylin-eosin (h&e) and the warthin-starry silver impregnation method, and immunostained with rabbit anti-h. pylori polyclonal antibody. helicobacter-like organisms (hlos) an ... | 2005 | 15940705 |
| isolation and preliminary characterization of a novel helicobacter species from swine. | to determine whether a helicobacter sp similar to helicobacter pylori in the stomachs of humans could be isolated from the stomachs of pigs. | 2005 | 16008213 |
| helicobacter pylori and extragastric diseases--other helicobacters. | the involvement of helicobacter pylori in the pathogenesis of extragastric diseases continues to be an interesting topic in the field of helicobacter-related pathology. although conflicting findings have been reported for most of the disorders, a role of h. pylori seems to be important especially for the development of cardiovascular and hematologic disorders. previously isolated human and animal helicobacter sp. flexispira and "helicobacter heilmannii" strains have been validated using polyphas ... | 2005 | 16178972 |
| role of surfactant protein d (sp-d) in innate immunity in the gastric mucosa: evidence of interaction with helicobacter pylori lipopolysaccharide. | surfactant protein d (sp-d) is a collagenous glycoprotein, a collectin, which functions as a pathogen-associated molecular pattern (pamp) recognition receptor in the innate immune response. although originally identified in the lung as a component of surfactant, sp-d also occurs in the gastric mucosa at the luminal surface and within gastric pits of mucus-secreting cells. infection with the gastroduodenal pathogen helicobacter pylori up-regulates expression of sp-d in human patients with gastrit ... | 2005 | 16303091 |
| helicobacter anseris sp. nov. and helicobacter brantae sp. nov., isolated from feces of resident canada geese in the greater boston area. | numbers of nonmigratory canada geese have increased substantially in the past decade, and they have become a nuisance in some urban areas. because of their close contact with humans in parks and areas adjacent to surface waterways, contact with their feces poses a zoonotic risk. a total of 97 geese from 10 separate geographic locales in the greater boston area had their feces sampled for detection of helicobacter spp. identification of helicobacter spp. based on 16s rrna genus-specific helicobac ... | 2006 | 16820454 |
| enterohepatic helicobacter species are prevalent in mice from commercial and academic institutions in asia, europe, and north america. | the discovery of helicobacter hepaticus and its role in hepatitis, hepatocellular carcinoma, typhlocolitis, and lower-bowel carcinoma in murine colonies was followed by the isolation and characterization of other helicobacter spp. involved in enterohepatic disease. colonization of mouse colonies with members of the family helicobacteriaceae has become an increasing concern for the research community. from 2001 to 2005, shipments of selected gift mice from other institutions and mice received fro ... | 2007 | 17507523 |
| helicobacter pylori isolated from patients with tonsillar cancer or tonsillitis chronica could be of different genotype compared to isolates from gastrointestinal tract. | helicobacter pylori from patients with different diseases, including so-called autoimmune thyroiditis, chronic tonsillitis and tonsillar cancer, was isolated and cultured. it was identified according to the genotype using labeled hybridization probes complementary to six sequences of caga and vaca genes. different types of strains were found in isolates from gastrointestinal tract and patients suffering from thyroiditis. six out of seven genotyped isolates from patients in our department of otor ... | 2007 | 17571803 |
| effect of helicobacter sp. infection on the number of antral gastric endocrine cells in swine. | the aim of the present study was to evaluate the effect of candidatus helicobacter suis (chs) and other helicobacter sp. different from candidatus helicobacter suis (non-candidatus helicobacter suis, non-chs) infection on the number of endocrine g and d cells and g/d cells ratio in antral gastric mucosa in swine. twenty nine stomachs were obtained from clinical healthy pigs about 6 months old and weighing approximately 100-120 kg after slaughter at abattoir located in central poland. from each s ... | 2007 | 17882928 |
| adherence, internalization, and persistence of helicobacter pylori in hepatocytes. | although helicobacter pylori have been identified in the liver, the role of helicobacter sp. in human liver diseases remains unclear. this study explored whether h. pylori were internalized and could persist in hepatocytes. the majority of an inoculum of h. pylori (1 x 10(7) colony forming units) adhered to hepatocytes. using the gentamicin invasion assay we found that approximately 2% were internalized and persisted following passage for more than 2 months. electron microscopy confirmed the pre ... | 2008 | 18320323 |
| specific and sensitive detection of h. pylori in biological specimens by real-time rt-pcr and in situ hybridization. | pcr detection of h. pylori in biological specimens is rendered difficult by the extensive polymorphism of h. pylori genes and the suppressed expression of some genes in many strains. the goal of the present study was to (1) define a domain of the 16s rrna sequence that is both highly conserved among h. pylori strains and also specific to the species, and (2) to develop and validate specific and sensitive molecular methods for the detection of h. pylori. we used a combination of in silico and mol ... | 2008 | 18648543 |
| helicobacter species are associated with possible increase in risk of hepatobiliary tract cancers. | attempts at early identification of hepatobiliary cancers have failed due to non-availability of a sensitive test. prevention offers the best chance to reduce the morbidity and mortality from these. identification of an etiological agent and especially if the potentially identified cause can be eradicated like helicobacter sp. infection, will not only reduce incidence but also the mortality. | 2009 | 18715780 |
| a commensal helicobacter sp. of the rodent intestinal flora activates tlr2 and nod1 responses in epithelial cells. | helicobacter spp. represent a proportionately small but significant component of the normal intestinal microflora of animal hosts. several of these intestinal helicobacter spp. are known to induce colitis in mouse models, yet the mechanisms by which these bacteria induce intestinal inflammation are poorly understood. to address this question, we performed in vitro co-culture experiments with mouse and human epithelial cell lines stimulated with a selection of helicobacter spp., including known p ... | 2009 | 19401779 |
| the dif/xer recombination systems in proteobacteria. | in e. coli, 10 to 15% of growing bacteria produce dimeric chromosomes during dna replication. these dimers are resolved by xerc and xerd, two tyrosine recombinases that target the 28-nucleotide motif (dif) associated with the chromosome's replication terminus. in streptococci and lactococci, an alternative system is composed of a unique, xer-like recombinase (xers) genetically linked to a dif-like motif (dif(sl)) located at the replication terminus. preliminary observations have suggested that t ... | 2009 | 19727445 |
| chronic hepatitis, hepatic dysplasia, fibrosis, and biliary hyperplasia in hamsters naturally infected with a novel helicobacter classified in the h. bilis cluster. | we recently described helicobacter-associated progressive, proliferative, and dysplastic typhlocolitis in aging (18- to 24-month-old) syrian hamsters. other pathogens associated with typhlocolitis in hamsters, clostridium difficile, lawsonia intracellularis, and giardia spp., were not indentified. the presence of helicobacter genus-specific dna was noted by pcr in cecal and paraffin-embedded liver samples from aged hamsters by the use of helicobacter-specific pcr primers. by 16s rrna analysis, t ... | 2009 | 19759229 |
| delineation of two helicobacter bilis genomospecies: implications for systematics and evolution. | the evolution and taxonomy of helicobacter bilis strains isolated in italy and finland were studied by phylogenetic analysis of different genes, comparative analysis of small rrna gene intervening sequence (ivs), amplified fragment length polymorphism analysis and dna-dna hybridization. the results of this study divided the h. bilis strains into two distinct and divergent genomic groups. in the absence of a specific phenotype or pathotype to distinguish these groups, however, they may be referre ... | 2010 | 19946059 |
| current understanding of spem and its standing in the preneoplastic process. | gastric cancer is the second leading cause of cancer-related death worldwide, but the details of gastric carcinogenesis remain unclear. in humans, two preneoplastic metaplasias are associated with the precancerous stomach: intestinal metaplasia and spasmolytic polypeptide-expressing metaplasia (spem). while mouse models of helicobacter sp. infection have not shown intestinal metaplasia, a number of mouse models lead to the evolution of spem. in this review, we summarize increasing data that indi ... | 2010 | 20047123 |
| could helicobacter organisms cause inflammatory bowel disease? | the discovery of helicobacter pylori sparked a revolution in the understanding and management of peptic ulcer disease and gastric cancer. other helicobacter species are recognized as important pathogenic agents in colitic diseases of rodents and primates, in particular helicobacter bilis, helicobacter fennelliae, helicobacter hepaticus and helicobacter trogontum. helicobacter bilis and h. hepaticus are now routinely used to initiate rodent models of inflammatory bowel disease (ibd), particularly ... | 2010 | 20955468 |
| helicobacter marmotae, novel helicobacter sp. and campylobacter sp. isolated from livers and intestines of prairie dogs. | prairie dogs (cynomys ludovicianus) are used to study the etiology and prevention of gallstones because of the similarities of prairie dog and human bile gallstone composition. epidemiologic and experimental studies have suggested a connection between helicobacter spp. infection and cholesterol cholelithiasis, cholecystis and gallbladder cancer. ten of thirty four prairie dogs in our study had positive helicobacter spp. identified by pcr with helicobacter genus-specific primers (c97, co5). fourt ... | 2011 | 21546560 |
| Comparative Genomics of Helicobacter pylori and the human-derived Helicobacter bizzozeronii CIII-1 strain reveal the molecular basis of the zoonotic nature of non-pylori gastric Helicobacter infections in humans. | ABSTRACT: | 2011 | 22039924 |
| immunodetection of helicobacter sp. and the associated expression of abo blood group antigens in the gastric mucosa of captive and free-living new world primates in the amazon region. | the histo-blood group abh antigens were first described in humans. these antigens are only present on erythrocytes from great apes and humans, while in more primitive animals they are found in tissues and body fluids. the abh antigens are mainly distributed in tissues exposed to the external environment and potentially serve as ligands for pathogens or inhibitors of tissue connections. the objective of this paper was two-fold: (i) to determine the presence of helicobacter sp. in the gastric muco ... | 2011 | 22241114 |