Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| deep scopulariopsosis: a case report and sensitivity studies. | a 36-year-old female was admitted to hospital for debridement of chronically inflamed tendon sheaths and adjacent tissues near the left ankle. despite antibiotic therapy and initial surgical interventions, the inflammation had progressed slowly over 16 months. histopathological examination of excised tissues in september 1973 revealed a chronic granulomatous inflammation of tendon sheaths and muscle. many branched hyphal segments, intercalary swollen cells, and a few conidia-like bodies were see ... | 1974 | 4139173 |
| the amino acid sequence of the trimethoprim-resistant dihydrofolate reductase specified in escherichia coli by r-plasmid r67. | the amino acid sequence of a trimethoprim-resistant dihydrofolate reductase (ec 1.5.1.3) specified by the r-plasmid r67 is described. the sequence was deduced from automatic and manual sequence analysis of the intact protein, the fragments produced by cyanogen bromide cleavage, and peptides derived from the largest cyanogen bromide fragment by digestion with trypsin, staphylococcus aureus v8 proteus, chymotrypsin, and lysobacter enzymogenes alpha-lytic protease. the complete sequence comprises 7 ... | 1979 | 387758 |
| extracellular nucleases of lysobacter enzymogenes: production of the enzymes and purification and characterization of an endonuclease. | lysobacter enzymogenes produced a nonspecific extracellular nuclease and an extracellular rnaase when grown in tryptone broth. both enzyme activities appeared after the exponential growth phase of the organism. the addition of rna to the medium specifically inhibited the production of the nuclease and the addition of phosphate prevented the synthesis of the rnaase. dna had no effect on the enzyme production. the lysobacter nuclease was purified 274-fold and its molecular weight was estimated to ... | 1980 | 6257361 |
| isolation and characterization of the plasma membrane and the outer membrane of deinococcus radiodurans strain sark. | deinococcus radiodurans strain sark, although gram-positive, has a complex cell wall profile that includes an outer membrane-like structure. the outer cell envelope layers formed blebs throughout the growth cycle, which were shed as large vesicles (0.5-3.5 micron m in diameter) from approximately 5% of the cell population. instability was accentuated by treatment with 10% nacl, which released the outer membrane from all cells without disrupting the peptidoglycan layer, and provided an outer memb ... | 1981 | 7296407 |
| catalytic mechanism of serine proteases: reexamination of the ph dependence of the histidyl 1j13c2-h coupling constant in the catalytic triad of alpha-lytic protease. | l-histidine, 90% 13c enriched at the c2 position, was incorporated into the catalytic triad of alpha-lytic protease (ec 3.4.21.12) with the aid of histidine-requiring mutant of lysobacter enzymogenes (atc 29487), and the ph dependence of the coupling constant between this carbon atom and its directly bonded proton was reinvestigated. the high degree of specific 13c isotopic enrichment attainable with the auxotroph permits direct observation and measurement of this coupling constant in proton-cou ... | 1981 | 7038675 |
| p-hydroxybenzoate hydroxylase from pseudomonas fluorescens. 1. completion of the elucidation of the primary structure. | as a final step in the elucidation of the primary structure of p-hydroxybenzoate hydroxylase from pseudomonas fluorescens, the amino acid sequences of a cnbr peptide (cb1, positions 111-276), that accounts for the middle part of the sequence, and the c-terminal cnbr peptide (cb2, positions 277-394) from the enzyme were determined. important sequence information was obtained from two subfragments that were formed by the cleavage with cnbr of the met-thr sequence (positions 346-347) in peptide cb2 ... | 1983 | 6406229 |
| use of endoproteinase lys-c from lysobacter enzymogenes in protein sequence analysis. | endoproteinase lys-c from lysobacter enzymogenes, which is commercially available, proved to be useful in the determination of primary structures of proteins. the enzyme preferentially cleaves at the carboxyl side of lysine residues. | 1983 | 6359954 |
| structure of mouse submaxillary gland renin. | to determine the structural basis for the highly specific action of renin, structural features of the active site and the complete amino acid sequence of mouse submaxillary gland renin were determined. a rapid method was developed for a large scale purification of renin from mouse submaxillary gland. the active site of renin was shown to consist of 2 aspartyl residues, 2 tyrosyl residues and one arginyl residue, the structures analogous to the active site of pepsin and other acid proteases. reni ... | 1983 | 6357566 |
| unusual sulfonolipids are characteristic of the cytophaga-flexibacter group. | capnocytophaga spp. contain a group of unusual sulfonolipids, called capnoids (w. godchaux iii and e. r. leadbetter, j. bacteriol. 144:592-602, 1980). one of these lipids, capnine, is 2-amino-3-hydroxy-15-methylhexadecane-1-sulfonic acid; the others are, apparently, n-acylated versions of capnine. the lipids were found, in amounts ranging from 2.5 to 16 mumol of capnoid sulfur per g of cells (wet weight), in two cytophaga spp. and also in several closely related organisms: several capnocytophaga ... | 1983 | 6298180 |
| cephabacins, new cephem antibiotics of bacterial origin. i. discovery and taxonomy of the producing organisms and fermentation. | three gram-negative bacteria produce new cephem antibiotics, named cephabacins, with unique 3-side chains. cephabacins include f group antibiotics with a 7-formylamino substituent and h group antibiotics without the substituent. the producing bacteria were taxonomically characterized and designated as lysobacter lactamgenus sp. nov. yk-90 and xanthomonas lactamgena sp. nov. yk-278 and yk-280. | 1984 | 6526722 |
| production of two phosphatases by lysobacter enzymogenes and purification and characterization of the extracellular enzyme. | lysobacter enzymogenes produces an extracellular phosphatase (ec. 3.1.3.1) during the stationary phase of growth. the cells also produce a cell-associated alkaline phosphatase. this enzyme is found in the particulate fraction of cell extracts and may be membrane bound. the production of both phosphatases, especially the extracellular enzyme, is reduced by inorganic phosphate. the extracellular phosphatase was purified to a specific activity of 270 u/mg primarily by chromatography on carboxymethy ... | 1984 | 6426386 |
| cephabacins, new cephem antibiotics of bacterial origin. ii. isolation and characterization. | fifteen components of new antibiotics, cephabacins, were isolated from the culture filtrates of lysobacter lactamgenus yk-90, xanthomonas lactamgena yk-280 and x. lactamgena yk-278. they were purified by column chromatography using cation-exchange resins, activated carbon, high porous resins and cation-exchange sephadex and by preparative reverse-phase hplc. the basic, water-soluble antibiotics were characterized as having a cephem skeleton and oligopeptide(s) as a side chain constituent from th ... | 1984 | 6526723 |
| refined structure of alpha-lytic protease at 1.7 a resolution. analysis of hydrogen bonding and solvent structure. | the structure of alpha-lytic protease, a serine protease produced by the bacterium lysobacter enzymogenes, has been refined at 1.7 a resolution. the conventional r-factor is 0.131 for the 14,996 reflections between 8 and 1.7 a resolution with i greater than or equal to 2 sigma (i). the model consists of 1391 protein atoms, two sulfate ions and 156 water molecules. the overall root-meansquare error is estimated to be about 0.14 a. the refined structure was compared with homologous enzymes alpha-c ... | 1985 | 3900416 |
| distribution of multicopy single-stranded dna among myxobacteria and related species. | multicopy single-stranded dna (msdna) is a short single-stranded linear dna originally discovered in myxococcus xanthus and subsequently found in stigmatella aurantiaca. it exists at an estimated 500 to 700 copies per chromosome (t. yee, t. furuichi, s. inouye, and m. inouye, cell 38:203-209, 1984). we found msdna in other myxobacteria, including myxococcus coralloides, cystobacter violaceus, cystobacter ferrugineus (cbfe17), nannocystis exedens, and nine independently isolated strains of m. xan ... | 1985 | 3932332 |
| catacandins, novel anticandidal antibiotics of bacterial origin. | two novel antibiotics, catacandin a and catacandin b, were isolated from the fermentation broth of the bacterium, lysobacter gummosus, by extraction and adsorption, reverse-phase and gel filtration chromatography. on the basis of their physico-chemical properties, they are acyltetramic acids that are easily distinguishable from others in this class. catacandin a and catacandin b possess good anticandidal activity. | 1985 | 4093330 |
| evolution of the regulatory isozymes of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase present in the escherichia coli genealogy. | the evolutionary history of isozymes for 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) synthase has been constructed in a phylogenetic cluster of procaryotes (superfamily b) that includes escherichia coli. members of superfamily b that have been positioned on a phylogenetic tree by oligonucleotide cataloging possess one or more of four distinct isozymes of dahp synthase. dahp synthase-0 is insensitive to feedback inhibition, while dahp synthase-tyr, dahp synthase-trp, and dahp synthase-phe ... | 1986 | 2867085 |
| comparison of the phosphatases of lysobacter enzymogenes with those of related bacteria. | lysobacter enzymogenes atcc 29487 (uasm 495) produces an outer-membrane-associated phosphatase and an excreted phosphatase. the cell-associated enzyme was compared to phosphatases of nine other gram-negative gliding bacteria and to that of escherichia coli. the other three species of the genus lysobacter also produce a particulate, cell-associated phosphatase. antiserum prepared against the phosphatase from the outer membrane of l. enzymogenes effectively precipitated the phosphatases of two oth ... | 1987 | 2833562 |
| serine protease mechanism: structure of an inhibitory complex of alpha-lytic protease and a tightly bound peptide boronic acid. | the structure of the complex formed between alpha-lytic protease, a serine protease secreted by lysobacter enzymogenes, and n-tert-butyloxycarbonylalanylprolylvaline boronic acid (ki = 0.35 nm) has been studied by x-ray crystallography to a resolution of 2.0 a. the active-site serine forms a covalent, nearly tetrahedral adduct with the boronic acid moiety of the inhibitor. the complex is stabilized by seven hydrogen bonds between the enzyme and inhibitor with additional stabilization arising fro ... | 1987 | 3122831 |
| the alpha-lytic protease gene of lysobacter enzymogenes. the nucleotide sequence predicts a large prepro-peptide with homology to pro-peptides of other chymotrypsin-like enzymes. | alpha-lytic protease is a 19.8-kda protein secreted from the gram-negative bacterium lysobacter enzymogenes. we have cloned and sequenced the gene for this serine protease. the nucleotide sequence contains an open reading frame which codes for the 198-residue mature enzyme and a potential prepro-peptide, also of 198 residues. the cooh-terminal 49 residues of the pro-peptide are significantly homologous to the propeptides of streptomyces griseus proteases a and b. we suggest that this pro-peptide ... | 1988 | 3053694 |
| lysobactin, a novel antibacterial agent produced by lysobacter sp. ii. biological properties. | lysobactin, an antibiotic isolated from a strain of lysobacter, is 2 to 4-fold more active than vancomycin against aerobic and anaerobic gram-positive bacteria. included in the spectrum of lysobactin are staphylococci, streptococci, corynebacteria, clostridia and various other gram-positive anaerobic bacteria. the activity of lysobactin against aerobic and anaerobic gram-negative bacteria is poor. when given parenterally the compound was efficacious in systemic staphylococcal and streptococcal i ... | 1988 | 3209466 |
| lysobactin, a novel antibacterial agent produced by lysobacter sp. i. taxonomy, isolation and partial characterization. | a new antibacterial agent, lysobactin, has been isolated from a species of lysobacter (atcc 53042). the antibiotic was recovered from the lysobacter cell mass by extraction and reversed phase chromatography. lysobactin is a dibasic peptide with marked activity against gram-positive aerobic and anaerobic bacteria. | 1988 | 3209465 |
| molecular analysis of the gene encoding alpha-lytic protease: evidence for a preproenzyme. | a 1.7-kb ecori fragment containing the structural gene for alpha-lytic protease has been cloned from lysobacter enzymogenes 495 chromosomal dna: the first example of a gene cloned from this organism. the protein sequence deduced from the nucleotide sequence encoding this serine protease matches the published amino acid sequence [olson et al., nature 228 (1970) 438-442] precisely. sequence analysis and s1 mapping indicate that, like subtilisin [e.g., wells et al., nucleic acids res. 11 (1983) 791 ... | 1988 | 3234766 |
| structural analysis of specificity: alpha-lytic protease complexes with analogues of reaction intermediates. | to better understand the structural basis of enzyme specificity, the structures of complexes formed between alpha-lytic protease, an extracellular serine protease of lysobacter enzymogenes, and five inhibitory peptide boronic acids (r2-borox, where r2 is methoxysuccinyl-ala-ala-pro- and borox is the alpha-aminoboronic acid analogue of ala, val, ile, norleu, or phe) have been studied at high resolution by x-ray crystallography. the enzyme has primary specificity for ala in the p1 position of pept ... | 1989 | 2611204 |
| analysis of prepro-alpha-lytic protease expression in escherichia coli reveals that the pro region is required for activity. | the alpha-lytic protease of lysobacter enzymogenes was successfully expressed in escherichia coli by fusing the promoter and signal sequence of the e. coli phoa gene to the proenzyme portion of the alpha-lytic protease gene. following induction, active enzyme was found both within cells and in the extracellular medium, where it slowly accumulated to high levels. use of a similar gene fusion to express the protease domain alone produced inactive enzyme, indicating that the large amino-terminal pr ... | 1989 | 2646278 |
| an immunologically related family of apolipoproteins associated with triacylglycerol storage in the cruciferae. | the major apolipoproteins associated with oil-storage bodies have been isolated from the mature seeds of six different species of the family cruciferae. the apolipoproteins were all of molecular mass 19-20 kda. they were highly abundant in mature seed tissue, accounting for up to 20% total seed proteins, and were localized exclusively on the membranes of oil-storage bodies. antibodies were raised in rabbits and mice against the six purified apolipoproteins. in each case, the antibodies specifica ... | 1989 | 2774566 |
| the alpha-lytic protease pro-region does not require a physical linkage to activate the protease domain in vivo. | alpha-lytic protease, an extracellular serine protease of lysobacter enzymogenes 495, is synthesized as a pre-pro-protein. previously it has been shown that when expressed in escherichia coli, the protein is autocatalytically processed in the periplasmic space, and that the functional protease domain accumulates extracellularly. engineered proteins lacking the 166 amino-acid pro-region were enzymatically inactive and remained cell-associated. by independently expressing the pro- and protease dom ... | 1989 | 2507926 |
| the use of tween 20 in a sensitive turbidimetric assay of lipolytic enzymes. | a turbidimetric esterase assay was developed using a tween 20 solution in the presence of cacl2 and lysobacter enzymogenes esterase (ec 3.1.1.1) as the enzyme source. the reaction was followed by measuring the increase in the optical density at 500 nm (od500) due to the hydrolytic release of the fatty acids from tween 20 and their precipitation as the calcium salts. concentrations of 1.8% tween and 3 mm cacl2 were found to be optimal for the assay of 0.036 to 0.15 esterase units in a 4-ml reacti ... | 1989 | 2501015 |
| molecular cloning and nucleotide sequence of the beta-lytic protease gene from achromobacter lyticus. | two bacteriolytic enzymes secreted by achromobacter lyticus m497-1 were purified and identified as being very similar (considering their amino acid composition and n-terminal sequence) to alpha- and beta-lytic proteases from lysobacter enzymogenes. a 1.8-kb ecori fragment containing the structural gene for beta-lytic protease was cloned from a. lyticus chromosomal dna. the protein sequence deduced from the nucleotide sequence was identical to the known sequence of beta-lytic protease, except for ... | 1990 | 2228973 |
| beta-lactamase of lysobacter enzymogenes: induction, purification and characterization. | lysobacter enzymogenes produces an inducible beta-lactamase and induction with 100 micrograms ampicillin ml-1 resulted in an increase of more than 100-fold in enzyme activity. various other beta-lactam antibiotics also served as effective inducers. the enzyme was obtained from cells by osmotic shocking to release periplasmic components and it was purified primarily by ion-exchange chromatography and page. the beta-lactamase consists of one polypeptide with a molecular mass of about 28 kda and an ... | 1990 | 2118167 |
| the ostrich pituitary contains a major peptide homologous to mammalian chromogranin a(1-76). | a major peptide related to the nh2-terminal fragment (position 1 to 76) of mammalian chromogranin a was isolated from ostrich adenohypophyses following acid-acetone extraction. the complete amino acid sequence of the homogenous peptide was deduced following automatic edman degradation of the native peptide as well as of cnbr-, tryptic- and lysobacter-derived peptides. the 76 amino acid sequence is strikingly homologous to bovine (80.3% sequence identity), porcine (79.0%), human (79.0%) and rat ( ... | 1990 | 2188233 |
| the complete amino acid sequence of the major kunitz trypsin inhibitor from the seeds of prosopsis juliflora. | the major inhibitor of trypsin in seeds of prosopsis juliflora was purified by precipitation with ammonium sulphate, ion-exchange column chromatography on deae- and cm-sepharose and preparative reverse phase hplc on a vydac c-18 column. the protein inhibited trypsin in the stoichiometric ratio of 1:1, but had only weak activity against chymotrypsin and did not inhibit human salivary or porcine pancreatic alpha-amylases. sds-page indicated that the inhibitor has a mr of ca 20,000, and ief-page sh ... | 1991 | 1367792 |
| characterization of rarobacter faecitabidus protease i, a yeast-lytic serine protease having mannose-binding activity. | rarobacter faecitabidus protease i, a yeast-lytic serine protease, was characterized in order to elucidate the mechanism of lysis of yeast cells by this enzyme. the n-terminal amino acid sequence of the enzyme was found to be homologous to those of lysobacter enzymogenes alpha-lytic protease and streptomyces griseus proteases a and b around the catalytic his residue, showing that it is a mammalian type serine protease. in a study of its substrate specificity, it preferentially hydrolyzed the est ... | 1991 | 1778983 |
| nucleotide sequence and characterization of the gene for secreted alkaline phosphatase from lysobacter enzymogenes. | lysobacter enzymogenes produces an alkaline phosphatase which is secreted into the medium. the gene for the enzyme (phoa) was isolated from a recombinant lambda library. it was identified within a 4.4-kb ecori-bamh1 fragment, and its sequence was determined by the chain termination method. the structural gene consists of an open reading frame which encodes a 539-amino-acid protein with a 29-residue signal sequence, followed by a 119-residue propeptide, the 281-residue mature phosphatase, and a 1 ... | 1991 | 1856159 |
| correct folding of alpha-lytic protease is required for its extracellular secretion from escherichia coli. | alpha-lytic protease is a bacterial serine protease of the trypsin family that is synthesized as a 39-kd preproenzyme (silen, j. l., c. n. mcgrath, k. r. smith, and d. a. agard. 1988. gene (amst.). 69: 237-244). the 198-amino acid mature protease is secreted into the culture medium by the native host, lysobacter enzymogenes (whitaker, d. r. 1970. methods enzymol. 19:599-613). expression experiments in escherichia coli revealed that the 166-amino acid pro region is transiently required either in ... | 1992 | 1618906 |
| characterization of the gene encoding the glutamic-acid-specific protease of streptomyces griseus. | the complete gene sequence (spre) for the glutamic-acid-specific serine protease (sgpe) of the gram-positive bacterium streptomyces griseus is reported. the spre gene encodes a 355 amino acid pre-pro-mature enzyme. the presence of a glutamic acid residue at the junction of the pro and mature segments of the protein suggests that the enzyme is self-processing. sgpe was found to have extensive homology with the s. griseus proteases a and b. however, there is an additional segment to the pro region ... | 1993 | 7910748 |
| phosphate-starvation induced changes in thiobacillus ferrooxidans. | we have analysed the response of the acidophilic chemolithotroph thiobacillus ferrooxidans to phosphate starvation. cultivation of the bacteria in the absence of added phosphate induced a remarkable filamentation of the cells. polyacrylamide gel electrophoresis revealed several proteins whose levels increased upon phosphate limitation, as well as some polypeptides that were exclusively synthesized under this growth limitation. one of the proteins whose level increased by the lack of phosphate wa ... | 1993 | 8472923 |
| beta-lactamase of lysobacter enzymogenes: cloning, characterization and expression of the gene and comparison of the enzyme to other lactamases. | the gene for the periplasmic beta-lactamase of lysobacter enzymogenes was isolated as part of a 1017 bp ecori fragment and the nucleotide sequence of the gene was determined. it has a g+c content of 71.5% and encodes a 27 amino acid signal sequence and the mature beta-lactamase of 276 amino acids which has a mass of 29,146 da. the enzyme appears to be unique to l. enzymogenes but its amino acid sequence shows a high degree of homology with the amino acid sequences of the lactamase from citrobact ... | 1993 | 8360618 |
| purification and characterization of a novel zinc-proteinase from cultures of aeromonas hydrophila. | while searching for an enzyme capable of breaking epsilon-(gamma-glu)-lys isopeptide bonds cross-linking protein chains, we purified a metallo-proteinase which mimics the action of an isopeptidase on the gamma-chain dimers of cross-linked fibrin. the enzyme is present in the growth medium of the bacterium aeromonas hydrophila, isolated from the intestinal tract of the leech hirudo medicinalis. it is a 19-kda protein which specifically hydrolyzes the gly-ala peptide bond within the gly-gly-ala se ... | 1993 | 8473348 |
| purification and characterization of alkaline serine protease from an alkalophilic streptomyces sp. | sap, an extracellular alkaline serine protease produced by streptomyces sp. ysa-130, was purified to homogeneity by cm-sephadex column chromatography and crystallization. the enzyme was a monomeric protein with a molecular weight of 19,000 as estimated by sds-page and gel filtration. the amino acid composition and amino-terminal sequence of sap were similar to those of other bacterial serine proteases, i.e., streptomyces griseus proteases a and b, lysobacter enzymogenes alpha-lytic protease and ... | 1994 | 7764689 |
| streptomyces griseus protease c. a novel enzyme of the chymotrypsin superfamily. | in this report we describe a novel chymotrypsin-like serine protease produced by streptomyces griseus. the enzyme has been tentatively named s. griseus protease c (sgpc). the gene encoding the enzyme (sprc) was identified and isolated on the basis of its homology to the previously characterized s. griseus protease b (sgpb). the sprc gene encodes a 457-amino acid prepro-mature protein of which only the 255 carboxyl-terminal amino acids are present in the mature enzyme. mature sgpc contains two di ... | 1994 | 8051104 |
| protease evolution in streptomyces griseus. discovery of a novel dimeric enzymes. | this report describes the cloning and sequencing of a novel protease gene derived from streptomyces griseus. also described is the heterologous expression of the gene in bacillus subtilis and characterization of the gene product. the sprd gene encodes a prepro mature protease of 392 amino acids tentatively named s. griseus protease d (sgpd). a significant component of the enzyme preregion was found to be homologous with the mitochondrial import signal of hsp60. the sprd gene was subcloned into a ... | 1995 | 7706307 |
| identification of genes encoding for peptide synthetases in the gram-negative bacterium lysobacter sp. atcc 53042 and the fungus cylindrotrichum oligospermum. | genes encoding for the multifunctional peptide synthetases lysobactin synthetase and peptolide sdz 214-103 synthetase were identified by hybridization of genomic libraries with oligonucleotides derived from consensus motifs of various genes encoding for delta-(l-alpha amino-adipoyl)-l-cysteinyl-d-valine (acv) synthetases and gramicidin s synthetase. the sequence of subcloned gene fragments revealed core motifs and a modular structure typical for the family of peptide synthetase genes. a fragment ... | 1996 | 8988370 |
| organization and expression in pseudomonas putida of the gene cluster involved in cephalosporin biosynthesis from lysobacter lactamgenus yk90. | the lysobacter lactamgenus yk90 pcbab gene encoding delta-(l-alpha-aminoadipyl)-l-cysteinyl-d-valine (acv) synthetase is located immediately upstream of the pcbc gene in the same orientation in the gene cluster involved in cephalosporin biosynthesis. the pcbab gene encodes a large polypeptide composed of 3722 amino acid residues with a molecular mass of 411593 da. the predicted amino acid sequence has a high degree of similarity with those of known acv synthetases from fungi and actinomycetes. w ... | 1996 | 8737573 |
| development of techniques for the genetic manipulation of the gliding bacteria lysobacter enzymogenes and lysobacter brunescens. | lysobacter enzymogenes and lysobacter brunescens are gram-negative gliding bacteria that belong to the gamma subgroup of the proteobacteria. as a first step toward a molecular analysis of lysobacter gliding motility, we developed techniques to genetically manipulate these bacteria. cosmid psup106 of the broad host range incompatibility group q (inc q) was introduced into l. enzymogenes and l. brunescens by conjugation and electroporation. psup106 replicated stably in both organisms and conferred ... | 1996 | 8864212 |
| molecular analysis of the gene cluster involved in cephalosporin biosynthesis from lysobacter lactamgenus yk90. | determination of the nucleotide sequence downstream from the lysobacter lactamgenus pcbc gene encoding isopenicillin n synthase revealed that five open-reading frames (orf) including the pcbc gene were tightly linked in the same orientation. each orf has the remarkable feature of the protein-coding frame in the dna sequence with a high g+c content. expression in escherichia coli and a comparison of the deduced amino acid sequences with published sequences showed that the gene cluster contained a ... | 1996 | 8703429 |
| cloning of a lysobacter enzymogenes gene that encodes an arginyl endopeptidase (endoproteinase arg-c). | screening an expression library of lysobacter enzymogenes dna allowed us to clone a gene encoding a serine protease that cleaves synthetic substrates c-terminal to arg and, to a lesser extent, lys residues. the gene product, which shares sequence homology with the lysyl endopeptidases from l. enzymogenes and achromobacter lyticus, consists of a signal sequence (24 residues), pro-region ( approximately 195 residues), and catalytic domain ( approximately 244 residues). downstream of this gene is a ... | 1998 | 9878833 |
| a new anti-mrsa antibiotic complex, wap-8294a. i. taxonomy, isolation and biological activities. | wap-8294a, produced by lysobacter sp., is a complex consisting of water soluble depsipeptide antibiotics. it was further purified by column chromatographies and hplc, and 19 components were obtained. wap-8294a2, a major component, and minor components a1, a4, ax8, ax9 and ax13 were active against gram-positive bacteria, in particular, methicillin-resistant staphylococcus aureus (mrsa) in vitro. wap-8294a2 was highly active in vivo in mice against the systemic infection of mrsa. | 1998 | 9917006 |
| biosynthesis and molecular genetics of cephamycins. cephamycins produced by actinomycetes. | cephamycin c is produced in a nine steps pathway by the actinomycetes s. clavuligerus and n. lactamdurans. the genes encoding the biosynthesis enzymes are clustered in both microorganisms as well as in the cephabacin producer lysobacter lactamgenus, a gram negative bacterium. the clusters of genes include genes encoding enzymes common to the biosynthesis of penicillin and cephalosporin c by the eukaryotic producers penicillium chrysogenum and cephalosporium acremonium and genes for steps specifi ... | 1999 | 10422584 |
| molecular cloning and cell-cycle-dependent expression of the acetyl-coa synthetase gene in tetrahymena cells. | to identify transcriptionally regulated mediators associated with the cell cycle, we adopted the differential mrna display technique for cell cultures of tetrahymena pyriformis synchronized by cyclic heat treatment. one cdna fragment that was expressed differently during synchronous cell division had a greatly decreased expression at 30 min after the end of heat treatment (eht). using this fragment as a probe, we isolated the full-length cdna for t. pyriformis acetyl-coa synthetase (tpacs) which ... | 1999 | 10510317 |
| two energetically disparate folding pathways of alpha-lytic protease share a single transition state. | the lysobacter enzymogenes alpha-lytic protease (alphalp) is synthesized with a 166 amino acid pro region (pro) that catalyzes the folding of the 198 amino acid protease into its native conformation. an extraordinary feature of this system is the very high energy barrier (deltag = 30 kcal mol-1) that effectively prevents alphalp from folding in the absence of pro (t1/2 = 1800 years). a pair of mutations has been isolated in the protease that completely suppresses the catalytic defect incurred in ... | 2000 | 10802737 |
| separation of wap-8294a components, a novel anti-methicillin-resistant staphylococcus aureus antibiotic, using high-speed counter-current chromatography. | the wap-8294a complex was isolated from the fermentation broth of lysobacter sp. wap-8294, whose major component, wap-8294a2, showed a strong activity against gram-positive bacteria including methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococci in vitro, and also exhibited a potent activity against mrsa in vivo. the previous separation procedure using the conventional chromatographic methods was laborious and time-consuming, and the recovery of the desired comp ... | 2001 | 11695870 |
| identification of precursor forms of free prostate-specific antigen in serum of prostate cancer patients by immunosorption and mass spectrometry. | the structural features of the free prostate-specific antigen (f-psa) present in human blood have not been clarified up to now, and it is, therefore, not known why f-psa is not complexed by the protease inhibitors that are present in human blood in large amounts. this lack of information is mainly attributable to the low amount of f-psa in serum, which makes the isolation and structural characterization very difficult, especially when only limited amounts of individual sera are available. it has ... | 2001 | 11221890 |
| characterization of an endoprotease (prpl) encoded by a pvds-regulated gene in pseudomonas aeruginosa. | the expression of many virulence factors in pseudomonas aeruginosa is dependent upon environmental conditions, including iron levels, oxygen, temperature, and osmolarity. the virulence of p. aeruginosa pao1 is influenced by the iron- and oxygen-regulated gene encoding the alternative sigma factor pvds, which is regulated through the ferric uptake regulator (fur). we observed that overexpression of pvds in strain pao1 and a deltapvds::gm mutant resulted in increased pyoverdine production and prot ... | 2001 | 11500408 |
| [structure and functions of bacterial proteinase precursors]. | the data on the precursors of bacterial proteases were generalized. the structure and special features of processing of the precursors of bacillary subtilisins, the alpha-lytic protease from lysobacter enzymogenes and the related chymotrypsin-like proteases from streptomyces griseus, and the metalloproteases from bacilli and pseudomonas aeruginosa were discussed. the approaches to producing the precursors and the protease propeptides and to in vitro characterizing them were particularly analyzed ... | 2001 | 11641907 |
| biosynthetic pathway of cephabacins in lysobacter lactamgenus: molecular and biochemical characterization of the upstream region of the gene clusters for engineering of novel antibiotics. | the cephabacins, one of the beta-lactam antibiotics, are produced by lysobacter lactamgenus. the previous studies the cephabacin biosynthesis were limited to a gene cluster that encodes the gene products responsible for the biosynthesis of the cephem nucleus. the long-term goal of this research is to elucidate the metabolic diversity and biosynthetic pathway of cephabacins and to design and/or discover new pharmacologically active compounds by engineering the cephabacin biosynthetic pathway in l ... | 2001 | 11676571 |
| tripropeptins, novel antimicrobial agents produced by lysobacter sp. i. taxonomy, isolation and biological activities. | peptide antibiotics tripropeptins a, b, c, d and z were isolated from cultured cells and broth of lysobacter sp. the differences among these components are in the lengths of the alkyl side chain. tripropeptins are active against gram-positive bacteria including mrsa in vitro. bactericidal activity of tripropeptin c disappeared in the simultaneous presence of chloramphenicol, a bacteriostatic agent. | 2001 | 11858660 |
| lysobacter strain with high lysyl endopeptidase production. | a new lysyl endopeptidase producing strain, lysobacter sp. ib-9374, was isolated from soil. this strain secreted the endopeptidase to culture medium at 6-12-fold higher levels relative to achromobacter lyticus and lysobacter enzymogenes. the mature lysobacter sp. enzyme was enzymatically identical to achromobacter lysyl endopeptidase bearing lysyl bond specificity, a high peptidase activity, a wide ph optimum, and stability against denaturants. nucleotide sequence analysis of the lysobacter sp. ... | 2002 | 12127482 |
| production, purification and characterization of an extracellular keratinase from lysobacter ncimb 9497. | the aim of this study was to determine the keratinolytic ability of a range of bacteria and subsequently, to characterize the keratinase showing the greatest biotechnological potential. | 2002 | 11967055 |
| bacteria associated with cysts of the soybean cyst nematode (heterodera glycines). | the soybean cyst nematode (scn), heterodera glycines, causes economically significant damage to soybeans (glycine max) in many parts of the world. the cysts of this nematode can remain quiescent in soils for many years as a reservoir of infection for future crops. to investigate bacterial communities associated with scn cysts, cysts were obtained from eight scn-infested farms in southern ontario, canada, and analyzed by culture-dependent and -independent means. confocal laser scanning microscopy ... | 2003 | 12514048 |
| [the structure of peptidoglycan from lysobacter sp.--a producer of extracellular bacteriolytic enzymes]. | 2003 | 12698805 | |
| taxonomic positioning of two biological control agents for plant diseases as lysobacter enzymogenes based on phylogenetic analysis of 16s rdna, fatty acid composition and phenotypic characteristics. | to taxonomically position two bacterial strains conferring biological control activity towards plant diseases. | 2003 | 12752818 |
| molecular characterization and expression in escherichia coli of three beta-1,3-glucanase genes from lysobacter enzymogenes strain n4-7. | lysobacter enzymogenes strain n4-7 produces multiple biochemically distinct extracellular beta-1,3-glucanase activities. the glua, glub, and gluc genes, encoding enzymes with beta-1,3-glucanase activity, were identified by a reverse-genetics approach following internal amino acid sequence determination of beta-1,3-glucanase-active proteins partially purified from culture filtrates of strain n4-7. analysis of glua and gluc gene products indicates that they are members of family 16 glycoside hydro ... | 2003 | 12867444 |
| characterisation of lysobacter enzymogenes (christensen and cook 1978) strain 3.1t8, a powerful antagonist of fungal diseases of cucumber. | isolate 3.1t8 of lysobacter enzymogenes (christensen and cook 1978), originating from the rhizosphere of cucumber and shown to have the potential to control pythium aphanidermatum, is described. the strain produces extracellular proteases and lipases and shows high levels of resistance against streptomycin, kanamycin and tetracycline, but not to chloramphenicol. it shows strong in vitro antibiosis against p. aphanidermatum and several other phytopathogenic fungi. in order to identify the isolate ... | 2003 | 12906383 |
| purification, bacteriolytic activity, and specificity of beta-lytic protease from lysobacter sp. ib-9374. | lysobacter sp. ib-9374, which was isolated from soil as a high lysyl endopeptidase-producing strain (chohnanet al., fems microbiol. lett., 213, 13-20, 2002), was found to produce a beta-lytic protease capable of lysing gram-positive bacteria such as staphylococcus aureus, microccocuseus, and bacillus subtilis. the lysobacter strain secreted the beta-lytic protease into the culture medium at a 2.4-fold higher level than achromobacter lyticus. the enzyme was highly purified through a series of six ... | 2003 | 16233362 |
| induced resistance as a mechanism of biological control by lysobacter enzymogenes strain c3. | abstract induced resistance was found to be a mechanism for biological control of leaf spot, caused by bipolaris sorokiniana, in tall fescue (festuca arundinacea) using the bacterium lysobacter enzymogenes strain c3. resistance elicited by c3 suppressed germination of b. sorokiniana conidia on the phylloplane in addition to reducing the severity of leaf spot. the pathogen-inhibitory effect could be separated from antibiosis by using heat-inactivated cells of c3 that retained no antifungal activi ... | 2003 | 18944093 |
| [mechanism of action of the extracellular bacteriolytic enzymes of lysobacter sp. on gram-positive bacteria: role of the cell wall anionic polymers of the target bacteria]. | the study of the extracellular bacteriolytic enzymes of lysobacter sp. showed that they can efficiently hydrolyze the peptidoglycan of gram-positive bacteria provided that there is an electrostatic interaction of these enzymes with the cell wall anionic polymers, teichoic and teichuronic acids in particular. the hydrolytic action of bacteriolytic enzymes on the cell wall largely depends on the negative charge of teichoic and teichuronic acids, rather than on their chemical composition. | 2004 | 15521173 |
| a second lysine-specific serine protease from lysobacter sp. strain ib-9374. | a second lysyl endopeptidase gene (lepb) was found immediately upstream of the previously isolated lepa gene encoding a highly active lysyl endopeptidase in lysobacter genomic dna. the lepb gene consists of 2,034 nucleotides coding for a protein of 678 amino acids. amino acid sequence alignment between the lepa and lepb gene products (lepa and lepb) revealed that the lepb precursor protein is composed of a prepeptide (20 amino acids [aa]), a propeptide (184 aa), a mature enzyme (274 aa), and a c ... | 2004 | 15262946 |
| structural investigations and identification of the extracellular bacteriolytic endopeptidase l1 from lysobacter sp. xl1. | the n-terminal amino acid sequence (23 amino acid residues) and the amino acid composition of the extracellular bacteriolytic enzyme l1 of 21 kd from the bacterium lysobacter sp. xl1 have been determined. the enzyme was hydrolyzed by trypsin, the resulting peptides were isolated, and their primary structures were determined. a high extent of homology (92%) of the n-terminal amino acid sequence and the primary structure of isolated peptides of the enzyme l1 (62 amino acid residues or 31% of prote ... | 2004 | 15193123 |
| [the effect of the extracellular bacteriolytic enzymes of lysobacter sp. on gram-negative bacteria]. | the effect of the extracellular bacteriolytic enzymes of lysobacter sp. on gram-negative bacteria was studied. these enzymes were found to be able to hydrolyze the peptidoglycan that was isolated from the gram-negative bacteria, the hydrolysis being completely inhibited by the cell wall lipopolysaccharide of these bacteria. the native cells of the gram-negative bacteria became susceptible to the bacteriolytic enzymes after the permeability of the outer membrane of the cells had been altered by t ... | 2004 | 15315224 |
| tripropeptin e, a new tripropeptin group antibiotic produced by lysobacter sp. bmk333-48f3. | 2004 | 15323129 | |
| tripropeptins, novel antimicrobial agents produced by lysobacter sp. | planar structures of tripropeptins (tpps) were elucidated by spectroscopic studies including various nmr measurements. stereochemistry of constituent amino acids of tripropeptin c (tppc) (3) was identified by marfey's method except hydroxyproline which was determined by studies of nmr and cd spectra. the absolute structure of 3 was determined by analyses of the fragments obtained by birch reduction and libh4 reduction of 3. the configuration of the fatty acid, isolated from acid hydrolysate of 3 ... | 2004 | 15032486 |
| suppression of damping-off disease in host plants by the rhizoplane bacterium lysobacter sp. strain sb-k88 is linked to plant colonization and antibiosis against soilborne peronosporomycetes. | we previously demonstrated that xanthobaccin a from the rhizoplane bacterium lysobacter sp. strain sb-k88 suppresses damping-off disease caused by pythium sp. in sugar beet. in this study we focused on modes of lysobacter sp. strain sb-k88 root colonization and antibiosis of the bacterium against aphanomyces cochlioides, a pathogen of damping-off disease. scanning electron microscopic analysis of 2-week-old sugar beet seedlings from seeds previously inoculated with sb-k88 revealed dense coloniza ... | 2005 | 16000790 |
| a clp gene homologue belonging to the crp gene family globally regulates lytic enzyme production, antimicrobial activity, and biological control activity expressed by lysobacter enzymogenes strain c3. | lysobacter enzymogenes strain c3, a biological control agent for plant diseases, produces multiple extracellular hydrolytic enzymes and displays antimicrobial activity against various fungal and oomycetous species. however, little is known about the regulation of these enzymes or their roles in antimicrobial activity and biocontrol. a study was undertaken to identify mutants of strain c3 affected in extracellular enzyme production and to evaluate their biocontrol efficacy. a single mini-tn5-lacz ... | 2005 | 15640196 |
| the role of clp-regulated factors in antagonism against magnaporthe poae and biological control of summer patch disease of kentucky bluegrass by lysobacter enzymogenes c3. | a global regulator was previously identified in lysobacter enzymogenes c3, which when mutated, resulted in strains that were greatly reduced in the expression of traits associated with fungal antagonism and devoid of biocontrol activity towards bipolaris leaf-spot of tall fescue and pythium damping-off of sugarbeet. a clp gene homologue belonging to the crp gene family was found to globally regulate enzyme production, antimicrobial activity, and biological control activity expressed by lysobacte ... | 2005 | 16234871 |
| [effect of the proteolytic enzymes of bacillus licheniformis and the lysoamidase of lysobacter sp. xl1 on proteus vulgaris and proteus mirabilis]. | preparations of culture liquid of three bacullus licheniformis strains (s, 103, and 60.4) and the enzymatic preparation lysoamidase from culture liquid of lysobacter sp. strain xl1 actively lysed preliminarily autoclaved cells of gram-negative bacteria proteus vulgaris and p. mirabilis. living proteus cells treated with these enzymatic preparations were lysed during their subsequent autoclaving. inoculation of enzyme-treated proteus cells, taken either separately or in combination with one anoth ... | 2005 | 16240656 |
| isolation and characterization of a new extracellular bacteriolytic endopeptidase of lysobacter sp. xl1. | the previously unstudied bacteriolytic enzyme l(4) was isolated from the culture liquid of the bacterium lysobacter sp. xl1 in electrophoretically homogeneous state. the enzyme l(4) is a diaminopimelinoyl-alanine endopeptidase relative to peptidoglycan of lysobacter sp. xl1. the enzyme is an alkaline protein of approximately 21 kd. the n-terminal amino acid sequence of the enzyme has been determined - a v v n g v n y v gx t t a ... the maximal activity of the enzyme was observed in 0.05 m tris-h ... | 2005 | 16266276 |
| bacteria associated with spores of the arbuscular mycorrhizal fungi glomus geosporum and glomus constrictum. | spores of the arbuscular mycorrhizal fungi (amf) glomus geosporum and glomus constrictum were harvested from single-spore-derived pot cultures with either plantago lanceolata or hieracium pilosella as host plants. pcr-denaturing gradient gel electrophoresis analysis revealed that the bacterial communities associated with the spores depended more on amf than host plant identity. the composition of the bacterial populations linked to the spores could be predominantly influenced by a specific spore ... | 2005 | 16269696 |
| lysobacter concretionis sp. nov., isolated from anaerobic granules in an upflow anaerobic sludge blanket reactor. | the taxonomic positions of lysobacter species with validly published names and a novel strain ko07(t), which was newly isolated from an upflow anaerobic sludge blanket reactor treating wastewater from a brewery, were (re)estimated on the basis of results obtained by using a polyphasic taxonomy approach. phylogenetic inference based on 16s rrna gene sequences showed that strain ko07(t) and all lysobacter species with validly published names clustered together in a phylogenetic branch within the c ... | 2005 | 15879248 |
| evolution of beta-lactam biosynthesis genes and recruitment of trans-acting factors. | penicillins and cephalosporins belong chemically to the group of beta-lactam antibiotics. the formation of hydrophobic penicillins has been reported in fungi only, notably penicillium chrysogenum and emericella nidulans, whereas the hydrophilic cephalosporins are produced by both fungi, e.g., acremonium chrysogenum (cephalosporin c), and bacteria. the producing bacteria include gram-negatives and gram-positives, e.g. lysobacter lactamdurans (cephabacins) and streptomyces clavuligerus (cephamycin ... | 2005 | 15950251 |
| mutagenesis of beta-1,3-glucanase genes in lysobacter enzymogenes strain c3 results in reduced biological control activity toward bipolaris leaf spot of tall fescue and pythium damping-off of sugar beet. | abstract lysobacter enzymogenes produces extracellular lytic enzymes capable of degrading the cell walls of fungi and oomycetes. many of these enzymes, including beta-1,3-glucanases, are thought to contribute to the biological control activity expressed by several strains of the species. l. enzymogenes strain c3 produces multiple extracellular beta-1,3-glucanases encoded by the glua, glub, and gluc genes. analysis of the genes indicates they are homologous to previously characterized genes in th ... | 2005 | 18943787 |
| influence of lysobacter enzymogenes strain c3 on nematodes. | chitinolytic microflora may contribute to biological control of plant-parasitic nematodes by causing decreased egg viability through degradation of egg shells. here, the influence of lysobacter enzymogenes strain c3 on caenorhabditis elegans, heterodera schachtii, meloidogyne javanica, pratylenchus penetrans, and aphelenchoides fragariae is described. exposure of c. elegans to l. enzymogenes strain c3 on agar resulted in almost complete elimination of egg production and death of 94% of hatched j ... | 2006 | 19259452 |
| lysobacter enzymogenes subsp. enzymogenes christensen and cook 1978, l. enzymogenes subsp. cookii christensen 1978 and streptococcus casseliflavus (mundt and graham 1968) vaughan et al. 1978 should have been cited in the approved lists of bacterial names. request for an opinion. | lysobacter enzymogenes subsp. enzymogenes christensen and cook 1978, l. enzymogenes subsp. cookii christensen 1978 and streptococcus casseliflavus (mundt and graham 1968) vaughan et al. 1978 were inadvertently omitted from the approved lists of bacterial names. according to rule 24a, note 1, the authors request that these names be considered as included in these lists. | 2006 | 17082416 |
| substrate specificity of nonribosomal peptide synthetase modules responsible for the biosynthesis of the oligopeptide moiety of cephabacin in lysobacter lactamgenus. | lysobacter lactamgenus produces cephabacins, a class of beta-lactam antibiotics which have an oligopeptide moiety attached to the cephem ring at the c-3 position. the nonribosomal peptide synthetase (nrps) system, which comprises four distinct modules, is required for the biosynthesis of this short oligopeptide, when one takes the chemical structure of these antibiotics into consideration. the cpbi gene, which has been identified in a region upstream of the pcbab gene, encodes the nrps - polyket ... | 2006 | 16436071 |
| two novel species, lysobacter daejeonensis sp. nov. and lysobacter yangpyeongensis sp. nov., isolated from korean greenhouse soils. | two bacterial strains were isolated from greenhouse soils of daejeon and yangpyeong regions in korea. the strains, designated gh1-9t and gh19-3t, were gram-negative and aerobic, with rod-shaped cells. their dna g+c contents were 61.7 and 67.3 mol%, respectively. the major fatty acids of strain gh1-9t were iso-c16 : 0, iso-c15 : 0, iso-c14 : 0, iso-c17 : 1omega9c and iso-c11 : 0 3-oh and the major components of strain gh19-3t were iso-c16 : 0, iso-c15 : 0, c16 : 1omega7c alcohol, iso-c17 : 1omega ... | 2006 | 16627636 |
| identification of bacterial micropredators distinctively active in a soil microbial food web. | the understanding of microbial interactions and trophic networks is a prerequisite for the elucidation of the turnover and transformation of organic materials in soils. to elucidate the incorporation of biomass carbon into a soil microbial food web, we added 13c-labeled escherichia coli biomass to an agricultural soil and identified those indigenous microbes that were specifically active in its mineralization and carbon sequestration. rrna stable isotope probing (sip) revealed that uncultivated ... | 2006 | 16885285 |
| purification, characterization, and gene analysis of cellulase (cel8a) from lysobacter sp. ib-9374. | an enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium lysobacter sp. ib-9374, a high lysyl endopeptidase-producing strain. the enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated cel8a. the purified cel8a had a molecular mass of 41 kda, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. a ph optimum of 5.0 was found for the beta-1,4-glucanase activit ... | 2006 | 17031054 |
| dominance of lysobacter sp. in the rhizosphere of two coastal sand dune plant species, calystegia soldanella and elymus mollis. | bacterial diversity in the rhizosphere of beach morning glory (calystegia soldanella) and wild rye (elymus mollis), two of the major plant species inhabiting the coastal sane dune in tae-an, korea, was studied by the analysis of community 16s rrna gene clones. the amplified rdna restriction analysis (ardra) of the clones using haeiii exhibited significant differences in the community composition between the two plant species as well as regional differences, but also identified a specific ardra p ... | 2006 | 16652206 |
| mapping of an internal protease cleavage site in the ssy5p component of the amino acid sensor of saccharomyces cerevisiae and functional characterization of the resulting pro- and protease domains by gain-of-function genetics. | ssy5p is a 77-kda protein believed to be a component of the sps amino acid sensor complex in the plasma membrane of saccharomyces cerevisiae. ssy5p has been suggested to be a chymotrypsin-like serine protease that activates the transcription factor stp1p upon exposure of the yeast to extracellular amino acid. here we overexpressed and partially purified ssy5p to improve our understanding of its structure and function. antibodies against ssy5p expressed in escherichia coli were isolated and used ... | 2006 | 16524914 |
| lysobacter koreensis sp. nov., isolated from a ginseng field. | strain dae16t, a gram-negative, non-spore-forming, rod-shaped bacterium, was isolated from the soil of a ginseng field in south korea and characterized in order to determine its taxonomic position. 16s rrna gene sequence analysis revealed that strain dae16t belongs to the gammaproteobacteria and had the highest degree of sequence similarity to lysobacter gummosus atcc 29489t (97.1 %), lysobacter antibioticus dsm 2044t (96.6 %), lysobacter enzymogenes dsm 2043t (96.2 %), lysobacter concretionis k ... | 2006 | 16403891 |
| identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16s rrna gene sequencing and by microbiological culture. | it has been postulated that bacteria attached to the surface of prosthetic hip joints can cause localised inflammation, resulting in failure of the replacement joint. however, diagnosis of infection is difficult with traditional microbiological culture methods, and evidence exists that highly fastidious or non-cultivable organisms have a role in implant infections. the purpose of this study was to use culture and culture-independent methods to detect the bacteria present on the surface of prosth ... | 2007 | 17501992 |
| substrate specificity and some physicochemical properties of autolytic enzymes of the bacterium lysobacter sp. xl 1. | the substrate specificity of autolytic enzymes of the bacterium lysobacter sp. xl 1 has been established. the periplasmic enzyme a8, the cytosolic enzyme a1, and the enzyme a10 solubilized from the cell walls and membranes with triton x-100 exhibit glucosaminidase activity; the cytosolic enzyme a4 and the enzyme a9 solubilized from the cell walls and membranes with licl exhibit the muramidase activity. the cytosolic enzymes a3 and a6 have n-acetylmuramoyl-l-alanine amidase activity, and the enzy ... | 2007 | 17680768 |
| structure and biosynthesis of heat-stable antifungal factor (hsaf), a broad-spectrum antimycotic with a novel mode of action. | a screen for antifungal compounds from lysobacter enzymogenes strain c3, a bacterial biological control agent of fungal diseases, has previously led to the isolation of heat-stable antifungal factor (hsaf). hsaf exhibits inhibitory activities against a wide range of fungal species and shows a novel mode of antifungal action by disrupting the biosynthesis of a distinct group of sphingolipids. we have now determined the chemical structure of hsaf, which is identical to that of dihydromaltophilin, ... | 2007 | 17074795 |
| lysobacter niabensis sp. nov. and lysobacter niastensis sp. nov., isolated from greenhouse soils in korea. | two bacterial strains, designated gh34-4(t) and gh41-7(t), were isolated from greenhouse soil cultivated with cucumber. the bacteria were strictly aerobic, gram-negative, rod-shaped and oxidase- and catalase-positive. 16s rrna gene sequence analysis indicated that these strains belong to the genus lysobacter within the gammaproteobacteria. strain gh34-4(t) showed highest sequence similarity to lysobacter yangpyeongensis gh19-3(t) (97.5 %) and lysobacter koreensis dae16(t) (96.4 %), and strain gh ... | 2007 | 17329782 |
| lysobacter defluvii sp. nov., isolated from municipal solid waste. | a bacterial isolate obtained from soil from a municipal landfill site in india was characterized using a polyphasic taxonomic approach. the colonies of the isolate were found to be yellow and highly mucoid. comparative analysis of the 16s rrna gene sequence showed that this isolate constitutes a distinct phyletic line within the genus lysobacter, displaying >3 % sequence divergence with respect to recognized lysobacter species. the generic assignment was confirmed by chemotaxonomic data, which r ... | 2007 | 17473271 |
| use of plate-wash samples to monitor the fates of culturable bacteria in mercury- and trichloroethylene-contaminated soils. | with the ultimate aim of developing bioremediation technology that use the optimum bacterial community for each pollutant, we performed polymerase chain reaction (pcr)-denaturing gradient gel electrophoresis (dgge) and phylogenetic analysis and identified communities of culturable bacteria in hgcl(2)- and trichloroethylene (tce)-contaminated soil microcosms. pcr-dgge band patterns were similar at 0 and 1 ppm hgcl(2), but changes in specific bands occurred at 10 ppm hgcl(2). band patterns appeari ... | 2007 | 17940764 |
| arenimonas malthae sp. nov., a gammaproteobacterium isolated from an oil-contaminated site. | a gram-negative, rod-shaped bacterium (cc-jy-1(t)) was isolated on nutrient agar from a soil sample collected from an oil-contaminated site located in chyai county, taiwan. 16s rrna gene sequence analysis demonstrated that this isolate is unique, showing 96.7 % sequence similarity to the type strain of arenimonas donghaensis and similarities of 93.0-93.8 % to species of the genera thermomonas, lysobacter and silanimonas. the presence of ubiquinone q-8, a polar lipid profile consisting of the maj ... | 2007 | 18048725 |
| molecular identification of bacteria in bronchoalveolar lavage fluid from children with cystic fibrosis. | culture of bronchoalveolar lavage fluid (balf) is the gold standard for detection of pathogens in the lower airways in cystic fibrosis (cf). however, current culture results do not explain all clinical observations in cf, including negative culture results during pulmonary exacerbation and inflammation in the absence of pathogens. we hypothesize that organisms not routinely identified by culture occur in the cf airway and may contribute to disease. to test this hypothesis we used a culture-indep ... | 2007 | 18077362 |
| lysobacter spongiicola sp. nov., isolated from a deep-sea sponge. | an aerobic, gram-negative bacterium, strain kmm 329(t), was isolated from a deep-sea sponge specimen from the philippine sea and subjected to a polyphasic taxonomic investigation. comparative 16s rrna gene sequence analysis showed that strain kmm 329(t) clustered with the species of the genus lysobacter. the highest level of 16s rrna gene sequence similarity (97.0 %) was found with respect to lysobacter concretionis kctc 12205(t); lower values (96.4-95.2 %) were obtained with respect to the othe ... | 2008 | 18218933 |
| lysobacter ginsengisoli sp. nov., a novel species isolated from soil in pocheon province, south korea. | a gram-negative, aerobic, rod-shaped, nonspore-forming bacterial strain, designated gsoil 357t, was isolated from soil sample of a ginseng field in pocheon province (south korea). the isolate contained q-8 as the predominant ubiquinone and iso-c16:0, iso-c17:1 9c, and iso-c15:0 as the major fatty acids. the g+c content of the genomic dna was 69.3mol%. a phylogenetic analysis based on 16s rrna gene sequences revealed that strain gsoil 357t was most closely related to lysobacter gummosus (97.6%) a ... | 2008 | 18852503 |
| expression and characterization of polyketide synthase module involved in the late step of cephabacin biosynthesis from lysobacter lactamgenus. | the cephabacins produced by lysobacter lactamgenus are beta-lactam antibiotics composed of a cephem nucleus, an acetate residue, and an oligopeptide side chain. in order to understand the precise implication of the polyketide synthase (pks) module in the biosynthesis of cephabacin, the genes for its core domains, beta-ketoacyl synthase (ks), acyltransferase (at), and acyl carrier protein (acp), were amplified and cloned into the pet-32b(+) expression vector. the sfp gene encoding a protein that ... | 2008 | 18388458 |