Publications

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an investigation of duck circovirus and co-infection in cherry valley ducks in shandong province, china.the co-infection of duck circovirus (ducv) with riemerella anatipestifer (ra) or/and escherichia coli (e. coli) or/and duck hepatitis virus i (dhv-i) in cherry valley ducks in china's shandong province was investigated by using polymerase-chain-reaction (pcr)-based methods. for this study, 742 ducks sampled at random from 70 duck farms during 2006-2007 were examined using pcr and dot-blot hybridisation (dbh) tests. overall the ducv infection rate was 33.29%. compared with those at 2 weeks of age ...200918760549
prokaryotic expression and potential application of the truncated pcv-2 capsid protein.three pairs of specific primers were designed to amplify the f2-1, f2-2 and xf2-2 truncated sequences of orf2 which encodes the capsid protein of porcine circovirus type 2 (pcv-2). the f2-1 sequence had most of the nls region of orf2, but the f2-2 and xf2-2 genes had the nls region deleted. truncated genes were subcloned into pet-32a(+) vectors to construct recombinant fusion expression vectors. the vectors were then transformed into rosetta(de3) e. coli and expressed by induction of iptg. expre ...201020960305
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