Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| hypersensitivity to histamine and systemic anaphylaxis in mice with pharmacologic beta adrenergic blockade: protection by nucleotides. | the effects of exogenous nucleotides on the histamine hypersensitivity of pharmacologically beta-blocked mice were investigated. female hla-sw (icr) mice, 27-29 gm, were injected intraperitoneally with 20 to 100 mug of propranolol 45 min before intraperitoneal challenge with 1 mg histamine. these animals had a mortality which averaged approximately 80%. at various time intervals before histamine, doses of from 0.5 to 12 mumoles of nucleotides were administered intravenously. noncyclic nucleotide ... | 1976 | 184134 |
| prevalent serotypes of bordetella pertussis in non-vaccinated communities. | in many countries, the prevalent serotypes of bordetella pertussis have changed from a mixture of types 1,2,3 and 1,2 (organisms possessing antigen 2) to a predominance of type 1,3. the timing of the change in different countries is shown to be related to the introduction of mass-vaccination with material rich in antigens 1 and 2 but weak in, or devoid of, antigen 3. in several parts of the world, there have been outbreaks of type 1,3 infection in fully vaccinated children. non-vaccinated commun ... | 1976 | 185285 |
| [structure and biological activity of bordetella pertussis endotoxin (proceedings)]. | 1976 | 185848 | |
| [increase of recirculating immunocompetent lymphocytes induced by bordetella pertussis (proceedings)]. | 1976 | 185849 | |
| separation and characterization of two distinct hemagglutinins contained in purified leukocytosis-promoting factor from bordetella pertussis. | 1. the leukocytosis-promoting factor of bordetella pertussis was found to contain two hemagglutinins with different susceptibilities to papain and separable from each other by agarose gel filtration with tris - hcl buffer containing 1 m nacl. 2. one hemagglutinin, referred to as hemagglutinin ha, had a high hemagglutinating activity, but neither leukocytosis-promoting nor histamine-sensitizing activity. the other hemagglutinin, referred to as hemagglutinin lpf appeared to be identical with the l ... | 1976 | 186106 |
| immune response against hamster erythrocytes in the low-responder mouse strains. xi. strain difference in the effects of various microbial adjuvants. | enhancing and suppressing effects of microbial adjuvants were studied in female mice of the c3h/he, akr and sl strains. propionibacterium acnes, bordetella pertussis, bcg and yeast cell wall (ycw) were chosen as adjuvants. as antigens, we chose hamster erythrocytes (hrbc) which proved to be a weak antigen for mice. adjuvants were given on day --7, day 0 or day 3, and hrbc were injected on day 0. the results were as follows. 1) p. acnes facilitated igm and igg antibody production in akr mice and ... | 1976 | 186656 |
| crossed immunoelectrophoretic analysis of bordetella pertussis antigens and of corresponding antibodies in human sera. | fourty-four antigens were demonstrated in sonicated preparations of bordetella pertussis (b.p.), using crossed immunoelectrophoresis against antiserum obtained from rabbits. no qualititative differences between the four strains of the danish pertussis vaccine were found. in preparations of b.p. culture medium, purified with respect to lymphocytosis promoting factor (lpf) activity, one antigen was possibly related to lpf. in human sera, antibodies against five of the b.p. antigens were demonstrat ... | 1976 | 187009 |
| cross-reactions between bordetella pertussis and twenty-eight other bacterial species. | cross-reactions between b. pertussis and 28 other bacterial species were studied by various quantitative immunoelectrophoretic methods. a sonicated b. pertussis antigen preparation and a corresponding pooled rabbit antiserum were used as reference system. two of the b. pertussis antigens were cross-reactive with antigens from 17, respectively 19, other bacterial species mainly gram-negative species. as judged by absorption of antibodies, the degree of cross-reactivity of these b. pertussis antig ... | 1976 | 187010 |
| role of the genetics and physiology of bordetella pertussis in the production of vaccine and the study of host-parasite relationships in pertussis. | 1976 | 187020 | |
| [intracytoplasmic inclusions in bordetella pertussis]. | 1976 | 178493 | |
| the production of ige and igga antibodies in normal rats and rats infected with nippostrongylus brasiliensis. | the time courses of production of ige and igga homocytotropic antibodies were measured in wistar rats during a primary and secondary response to egg albumin with pertussis or freund's adjuvants. an anamnestic ige antibody response occurred in animals previously sensitized to antigen with killed bordetella pertussis as adjuvant. igga antibodies were formed in the primary response with freund's complete adjuvant only, but were found during the secondary response with all adjuvants used. the time ... | 1976 | 178591 |
| production of circulating reaginic (ige) antibodies by oral administration of ovalbumin to rats. | reaginic antibody synthesis following parenteral and/or oral administration of ovalbumin and bordetella pertussis organisms as adjuvant has been evaluated in lou/m/wsl inbred rats. these rats are able to produce high reaginic antibody serum levels after intraperitoneal injection of this antigen. primary oral administration of ovalbumin doses between 10 and 100 mg with bordetella pertussis organisms given as adjuvant by the intraperitoneal or the oral route led to characteristic reagnic responses ... | 1976 | 179939 |
| mouse or man? which are pertussis vaccines to protect? | type 1 strains of bordetella pertussis can infect mouse brain and have been recovered as type 1 organisms after death. when introduced into the naso-pharynx of the marmoset, they immediately acquired agglutinogen 2 or 3, and the resulting type 1,2 or 1,3 infection persisted for many weeks. as in the child, agglutinogens 2 and/or 3 appear to be essential for infection of the marmoset, whereas they are quite unnecessary in mouse brain. a vaccine (extract or whole cell) containing agglutinogen 1 ma ... | 1976 | 177701 |
| the importance of agglutinin production in mice in the determination of the definitive serotype of bordetella pertussis. | a schedule for the routine serotyping of strains of bordetella pertussis based on agglutinin production in mice to the k-antigens has been worked out. mice have been found as satisfactory as rabbits but far more economical for the production of the very small volumes of serum which are required. agglutinin production, used in conjunction with direct agglutination, provides definitive information about serotype. | 1976 | 177702 |
| the serotypes of bordetella pertussis isolated in great britain between 1941 and 1968 and a comparison with the serotypes observed in other countries over this period. | classification, by agglutinogens, of 634 isolates of bordetella pertussis collected from 1971 to 1968 in great britain demonstrated that a change from a predominantly 1,2,0,4 serotype (75% of those examined during 1941-4) to a predominantly 1,0,3,0 serotype (73% of those examined during 1966-8) occurred sometime after 1953. furthermore, evidence from the examination of isolates collected between 1941 and 1953 suggests that the change may have been gradual. isolates of serotype 1,2,3,4 made up 20 ... | 1976 | 177703 |
| the stability of the serotypes of bordetella pertussis with particular reference to serotype 1,2,3,4. | strains of bordetella pertussis in which all the organisms contain agglutinogens 1 and 3 or 1,2 and 4 are easy to identify as serotypes 1,0,3,0 and 1,2,0,4 respectively; and similarly, stable strains of serotype 1,0,3,4 are occasionally found. during repeated subcultures, passage in vivo, and lyophilization and preservation for many years, these serotypes do not change. mixing 1,0,3,0 and 1,2,0,4 serotypes and culturing them together in vivo and in vitro produces cultures from which organisms of ... | 1976 | 177704 |
| the effect of bordetella pertussis on rosette-forming cells present in the peritoneal fluids of mice. | 1976 | 177778 | |
| loss of protective antigen, histamine-sensitising factor and envelope polypeptides in cultural variants of bordetella pertussis. | five bordetella pertussis strains of phase i were grown in conventional casamino-acid medium and in media modified by adding high concentrations of mgso4 or nicotinic acid. cells grown in high-magnesium media (in the c-mode) had only about 4% of the protective antigen (pa) and 6% of the histamine-sensitising factor (hsf) of cells from the normal medium. envelopes from c-mode organisms when examined by sds-page showed a loss of 28k and 30k polypeptide bands. similar parallel losses of pa, hsf and ... | 1976 | 57240 |
| isolation and properties of the leukocytosis- and lymphocytosis-promoting factor of bordetella pertussis. | the leukocytosis- and lymphocytosis-promoting factor (lpf) of bordetella pertussis has been isolated to near homogeneity by physical, chemical, and electron microscopical criteria. lpf contains 14.5% nitrogen and is lipid and carbohydrate free. it is apparently composed of four polypeptide subunits. lpf caused leukocytosis and lymphocytosis in "nude" as well as in normal mice. in addition, purified lpf also induced histamine sensitization and hypoglycemia and refractoriness to the hyperglycemic ... | 1976 | 58054 |
| letter: protective activity of whooping-cough convalescent serum and serum-iga level in mice infected with bordetella pertussis. | 1976 | 59233 | |
| [vaccine from the cell fragments of bordetella pertussis. i. protective, sensitizing properties and morphological characteristics of the vaccine]. | the authors present the results of studying the protective and sensitizing properties of a new preparation made of a ultrasonic disintegrate of pertussis microbes treated by ethyl ether. as shown by electron microscopy, the preparation consisted of the cell wall elements (the membrane), remnants of the cytoplasm and protectosome, i.e. it represented a vaccine consisting of cell fragments. in crude and sorbed condition it possessed marked protective properties (a test on mice). the content of pro ... | 1976 | 64095 |
| enhancement of reaginic and hemagglutinating antibody production by an extract of bordetella pertussis containing histamine sensitizing factor. | the effect of an extract containing the histamine-sensitizing factor (hsf) of bordetella pertussis on the immune response of mice to ovalbumin was investigated with respect to dose of antigen and adjuvant. of particular interest was the enhancement of reaginic antibody production. in comparison to the al(oh)3 induced production of reaginic antibody where low doses of antigen and adjuvant yield high titers of reagin, the hsf extract demonstrated optimal adjuvant activity at high doses of both adj ... | 1976 | 173756 |
| characteristics of cells present in peritoneal fluids of mice injected intraperitoneally with bordetella pertussis. | peritoneal fluids obtained from mice after the intraperitoneal administration of bordetella pertussis vaccine, heated vaccine, an extract of the organisms, killed escherichia coli, or thioglycolate medium were examined in terms of total cells and percentage that adhered to glass cover slips during 2-h incubation period. all these substances were found to increase the number of leukocytes in peritoneal fluid within 1 to 2 days after the injection. this increase appeared to be due to an influx of ... | 1976 | 175017 |
| the effect of bordetella pertussis on the antibody response in mice to type iii pneumococcal polysaccharide. | the effect of an i.p. injection of bordetella pertussis on the primary humoral immune response in mice to the thymus-independent antigen siii has been studied. suppression of the antibody response occurred when pertussis cells were injected at the same time as an optimal immunizing dose of siii. in contrast, the antibody response to high doses of siii was enhanced by b. pertussis. when siii alone was injected, only 19s antibody was detected. however, when b. pertussis was administered with eithe ... | 1976 | 3610 |
| the influence of growth medium on the interactions between bordetella pertussis and staphylococcus aureus. | previous observers showed that many strains of staphylococcus aureus stimulated the growth of bordetella pertussis but we have found the reverse: the growth of all available strains of b. pertussis on charcoal-agar medium was inhibited by a standard strain of s. aureus; and 17 of 18 strains of s. aureus (as well as several other organisms) inhibited the growth of a standard strain of b. pertussis. all inhibiting colonies had an unusual brown colouration on the charcoal agar used in the investiga ... | 1976 | 6798 |
| histamine hypersensitivity in mice induced by bordetella pertussis or pharmacologic beta adrenergic blockade. effects of adrenergic, cholinergic, and other drugs. | the effects of prostaglandin e1, e2, f2alpha (pge2 pgf2alpha), isoproterenol, epinephrine, norepinephrine, salbutamol, practolol, atropine, aminophylline, and corticosterone on the hypersensitivity to anaphylaxis, histamine, and serotonin in bordetella pertussis-treated mice and propranolol-treated mice were investigated. female hla-sw (icr) mice, 27-29 gm, were injected with pertussis vaccine intravenously 4 days before challenge with antigen, histamine, or serotonin. alternatively, instead of ... | 1976 | 9437 |
| biologically active components and properties of bordetella pertussis. | 1976 | 11644 | |
| the effects of bordetella pertussis vaccine on cerebral vascular permeability. | the effect of bordetella pertussis vaccine on the cerebral vascular permeability in the mouse was studied by a radio-isotope method (131i-labelled hsa). intravenous injection of 4 x 1010 heat-killed pertussis organisms caused a measurable increase in permeability in normal mice. cryoinjury to the cerebral hemispheres resulted in a striking increase in vascular permeability at 24 h. this declined within 48 h and stabilized at a level fractionally higher than normal at 7 days ("healed lesion"). wh ... | 1976 | 12780 |
| adenyl cyclase in bordetella pertussis vaccines. | brodetella pertussis organisms have a large amount of extracytoplasmic adenyl cyclase, part of which is found in the supernatant culture medium during exponential growth. the enzyme differs from previously studied bacterial adenyl cyclases in biochemical characteristics as well as in location. several commercial pertussis vaccines were found to contain adenyl cyclase activity; this activity is probably due to the extracytoplasmic enzyme associated with and released from the whole cells in the va ... | 1977 | 19542 |
| the insulin-like action of bordetella pertussis vaccine in rats. | treatment of rats with bordetella pertussis vaccine significantly lowered the blood sugar level 4 days later but the vaccine did not alter the level in diabetic rats. the vaccine, like insulin, raised glycogen levels in liver, skeletal muscle and heart and reduced the plasma free fatty acid concentration. the action of a beta-adrenoceptor blocker was potentiated by the vaccine but not by insulin. part of the hypoglycaemic action of the vaccine is probably due to beta-adrenoceptor blockade. | 1977 | 22497 |
| rosette-forming mast cells in rat anaphylaxis. immunological characteristics of mast cell rosettes. | peritoneal mast cells from immunized rats can form rosettes with antigen-coated sheep red blood cells. the receptor responsible for this active rosette formation is shown to be ige cytophilic antibody: rosettes are inhibited by previous contact of mast cells with antigen, or with anti-ige antiserum; the kinetics of mast cell rosettes following a primary immunization with ovalbumin and bordetella pertussis vaccine is similar to the kinetics of reaginic antibody response. furthermore, a reaginic s ... | 1977 | 73505 |
| the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. i. proliferative response. | the lymphocytosis-promoting factor of bordetella pertussis is a potent mitogen for murine lymphocytes in vitro. the stimulatory response was not the result of specific antigen stimulation. spleen and lymph node cells were responsive, whereas normal thymocytes were unresponsive. however, dna replication was induced in cortisone-resistant thymocytes by lymphocytosis-promoting factor (lpf). bone marrow cells were not stimulated by lpf. | 1977 | 187715 |
| the in vitro effects of bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes: ii. nature of the responding cells. | the mitogenic response of murine lymphocytes to the lymphocytosis-promoting factor of bordetella pertussis has been shown to be due to activation of t cells. the selectivity of responsiveness to lpf with respect to the population of t cells which is stimllated, differs from that of pha as well as con a, and the surface receptors are different. a population of adherent cells, which does not appear to consist of macrophages or other phagocytic cells, is required for the t-cell response. | 1977 | 187716 |
| preferential enhancement of ige antibody formation by bordetella pertussis. | preferential enhancement of ige antibody response was observed in balf/c mice by the administration of bordetella pertussis with antigen (dnp-salmonella). correlation between b cell mitogenic activity and adjuvant action among b. pertussis, salmonella, lipopolysaccharide of escherichia coli and ficoll was examined but was not found. thymus-derived cells seemed necessary to develop adjuvant action of b. pertussis since antibody response in athymic nude mice was not influenced by b. pertussis. hel ... | 1977 | 194843 |
| 2-o-(beta-d-glucuronyl)-7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose: a constituent of the bordetella pertussis endotoxin. | the presence of bound d-glucuronic acid in the endotoxin of bordetella pertussis was demonstrated. the branched chain trisaccharide named in the title was isolated after hydrolysis of the endotoxin with 3 m hcl for 2 h at 100 degrees c. its structure was established by chemical and enzymic degradation. | 1977 | 196849 |
| identification of 2-methyl-3-hydroxydecanoic and 2-methyl-3-hydroxytetradecanoic acids in the 'lipid x' fraction of the bordetella pertussis endotoxin. | the 'lipid x' fraction, released from the bordetella pertussis endotoxin upon treatment with trifluoroacetic acid of ph 3 at 50 degrees c, was shown to contain, in addition to 3-hydroxydecanoic, 3-hydroxydodecanoic, 3-hydroxytetradecanoic, and tetradec-2-enoic acids, 2-methyl-3-hydroxydecanoic-, and 2-methyl-3-hydroxytetradecanoic acids. the structure of these was established by gas-liquid chromatography/mass spectrometry. | 1977 | 196857 |
| pertussis outbreak in austin and travis county, texas, 1975. | an outbreak of bacteriologically proven pertussis occurred in austin and travis county, texas, over a 7-month period in 1975. eighty persons were cultured for pertussis in our laboratory. a total of 62% of specimens from 34 individuals with suspected pertussis was positive for bordetella pertussis. diagnosis of acute cases by both culture and fluorescent antibody was attempted, and the correlation of the methods is given. analyses of cases by age, sex, immunization status, and antibiotic treatme ... | 1977 | 197117 |
| the mitogenic effect of the lymphocytosis promoting factor from bordetella pertussis on human lymphocytes. | the purified lymphocytosis promoting factor (lpf) from bordetella pertussis was found to be a potent mitogen for peripheral blood lymphocytes (pbl) from normal adults as well as for cord blood lymphocytes. proliferation occurred in autologous plasma or fetal calf serum, regardless of previous exposure to pertussis infection or immunization. only one adult human serum, from a physician constantly working with b. pertussis, inhibited the mitogenic response to lpf and this serum was shown to contai ... | 1977 | 197121 |
| pathogenesis of infection with bordetella pertussis in hamster tracheal organ culture. | hamster tracheal organ culture was employed as a model for study of the pathogenesis of infection due to bordetella pertussis. infected tracheal explants were examined with light, immunofluorescence, and electrom microscopy. b. pertussis organisms preferentially attached to the ciliated cells, producing ciliostasis and marked destruction of the subcellular organelles followed by expulsion of these cells from the epithelial layer. other nonciliated respiratory epithelial cells appeared to be unaf ... | 1977 | 197174 |
| lymphocytosis-promoting factor of bordetella pertussis: isolation, characterization, and biological activity. | the leukocytosis and lymphocytosis-promoting factor (lpf) of bordetella pertussis has been isolated in apparently pure form. lpf is a protein essentially free of lipid and carbohydrate with an estimated molecular weight of 67,000-73,600 daltons. purified lpf induced both histamine sensitization and refractoriness to epinephrine-induced hyperglycemia and was a murine thymus-derived (t-) cell mitogen. adenyl cyclase activity also appeared to be associated with lpf. | 1977 | 197175 |
| perfusion of the pancreas isolated from pertussis-sensitized rats: potentiation of insulin secretory responses due to beta-adrenergic stimulation. | in order to study the mechanism by which pertussis-sensitized rats showed enhanced insulin secretory responses to various secretagogues (sumi, t., and m. ui, endocrinology 97: 352, 1975), pancreases of rats receiving a single injection of bordetella pertussis cells 3 days before were perfused with krebs-ringer solution, and release of insulin therefrom was compared with that from the pancreases of normal rats. much more insulin was released from the pancreas of the pertussis-sensitized rat than ... | 1977 | 198199 |
| enrichment medium for the isolation of bordetella. | the development of a specimen collection and transport medium outfit for the rapid laboratory diagnosis of whoping cough is described. the transport medium consisted of a semisolid agar containing charcoal, cephalexin, and defibrinated horse blood. it was also found to be an excellent enrichment medium for the selective isolation of bordetella pertussis and b. parapertussis from scantily populated specimens. the investigation of 3,237 specimens that yielded 1,419 positive isolates of bordetella, ... | 1977 | 198430 |
| [adjuvant activity of gram-negative bacteria and their structural components (author's transl)]. | regarding the adjuvant activity of gram-negative bacteria we have to distinguish at least 4 different potencies, i.e., 1) increase in the production of circulating antibodies during the primary and secondary immune responses; 2) induction of susceptibility to systemic anaphylaxis; 3) prompt production of experimental "allergic" diseases, and 4) increase in resistance to infections. although all gram-negative bacteria contain several structural components with adjuvant potencies, the immunopotent ... | 1977 | 199540 |
| resistance of bordetella pertussis phase i to mucociliary clearance by rabbit tracheal mucous membrane. | cultivated mucous membrane of rabbit trachea was point inoculated with bordetella pertussis phase i or iii. phase i (virulent) bacteria were found to be infective at the point-inoculated site, but phase iii (avirulent) bacteria rarely showed such behavior. after inoculation, homogenized segments of mucous membrane were spread on plates. large numbers of phase i bacteria were recovered from the inoculated segment; however, the laryngeal segment was the site of recovery of large numbers of phase i ... | 1977 | 199673 |
| the influence of various methods of sensitization on delayed hypersensitivity to bordetella pertussis in the sprague dawley rat. | in the rat, the injection of bordetella pertussis produces, after prior sensitization, a delayed hypersensitivity inflammatory reaction within the pleural cavity. the influence of various methods of sensitization on this hypersensitivity was studied in the sprague dawley rat. the reaction was very variable according to the experimental conditions used. optimal sensitization was obtained following the injection of antigen mixed with complete freund's adjuvant into the dorsal surface of the paws. | 1977 | 200190 |
| oral feeding of ovalbumin can make rats tolerant to an intraperitoneal injection of dinitrophenylated ovalbumin and bordetella pertussis vaccine [proceedings]. | 1977 | 200504 | |
| scanning electron microscopic study of hamster tracheal organ cultures infected with bordetella pertussis. | hamster tracheal organculture was employed as a model for the study of the pathogenesis of infection due to bordetella pertusis. scanning electron microscopy provided a three-dimensional view of the surface infection of the tracheal explants. phase i b. pertussis attached only to the ciliated epithelial cells, and a sequence of events involving the injury, expulsion, and destruction of these differentiated cells occurred. this in vitro model provides insights into the mechanisms by which b. pert ... | 1977 | 200679 |
| [immunogenicity and toxicity of soluble trichloroacetic acid precipitate from pertussis microbes and its fractions]. | the authors studied the biological properties of the preparations of pertussis protective antigens obtained by the disintegration of the microbial mass of bordetella pertussis, with the subsequent purification with trichloracetic acid (tca-preparations). tca-preparations proved to possess a stable protective activity and by the ratio of the protective dose to toxic and histamine-sensitizing doses considerably exceeded the corpuscular vaccine. a tca-preparation fraction with a greater immunogenic ... | 1977 | 202117 |
| [determination of major 1, 2, and 3 agglutinant antigens of bordetella pertussis strains isolated from brazilian children with whooping-cough treated at the emilio ribas hospital of são paulo, brazil]. | 1977 | 203021 | |
| pertussis vaccine--fact and fiction. | the present paper indicates a number of unresolved problems in the metabolism of bordetella pertussis and in the standardization of pertussis vaccines. the use of chemically defined media should enable the metabolic problems to be clarified. the problems concerned with the control and standardization of vaccines can largely be resolved in the laboratory with the introduction of reference preparations but the question of the reactivity of vaccine remains for the future. | 1977 | 203169 |
| bordetella pertussis in the national capital region: prevalent serotype and immunization status of patients. | over a 2-year period 67 strains of bordetella pertussis were identified in 231 single specimens of nasopharyngeal secretions submitted from patients suspected to have whooping cough in the national capital region; 89.5% of the identifications were made by culture. serotype 1,3 was predominant. at least 75% of the patients with bacteriologically confirmed whooping cough had not been fully immunized. there was no evidence that adenoviruses or other viruses played any important etiologic role in th ... | 1977 | 203375 |
| [isolation and purification of a preparation possessing leukocytosis-stimulating properties from pertussis bacteria]. | a homogeneous protein lsf-2 preparation was extracted from the cultural fluid of bordetella pertussis strains of the 1.0.3 serological type by means of precipitation with ammonium sulphate and electrofocussing; this preparation proved to produce a marked leukocytosis-stimulating and a weak toxic action of delayed type in experiments on animals. intraperitoneal administration of 5 mug of the lsf-2 preparation caused a rise of leukocytosis in mice to 100,000 cells per 1 mm3, a delay in the gain in ... | 1977 | 190830 |
| in vitro stimulation of human lymphocytes by bordetella pertussis. | bordetella pertussis (b.p.) induces blast transformation of human lymphocytes; whole killed b.p. are more efficient than extracts obtained by sonication. similar responses were obtained with each of the four strains used in the danish pertussis vaccine. b.p. with low amounts of protective antigen and histamine-sensitizing factor also induced lymphocyte transformation, but were less toxic to the lymphocytes at high concentrations. the supernatants of b.p. cultures were purified with respect to ly ... | 1977 | 190855 |
| effects of bordetella pertussis on the sensitivity of inbred mice to vasoactive amines. | pretreatment with bordetella pertussis was determined to increase significantly the hypovolemia induced by intravenous injections of histamine either alone or in mixture with serotonin in a total of 26 different strains of mice. two factors affecting the mortality rates observed by challenge after b. pertussis treatment were: the sensitivity of the strains to vasoactive amines before b. pertussis treatment, and their resistance to acute hypovolemic shock. appropriate crosses and backcrosses betw ... | 1977 | 193799 |
| histopathological changes in mice treated with extracts of the histamine-sensitizing factor of bordetella pertussis. | the histamine-sensitizing factor (hsf) of bordetella pertussis was isolated in a highly purified form. in addition to inducing profound sensitization to histamine, it also caused a significant lymphocytosis and produced an enhancement of reaginic antibody production in animals upon immunization with antigen. biologically active doses of hsf produced significant pathological changes in mice including congestion and edema of the lung and a marked depletion of cells in the thymus, white pulp of the ... | 1977 | 193800 |
| study on toxicity of bordetella pertussis cultures and antigens. | the toxicity of 201 bordetella pertussis strains isolated in belgrade has been studied. the strains were grown on a modification of cohen and wheeler's medium. ld50 of examined strains ranging between 1.06 and 1.95 billion bacteria in 35 (17.41%), between 2.04 and 4.83 billion bacteria in 92 (45.77%) and between 5.31 and 7.79 billion bacteria in 46 (22.88%) were found. low toxic cultures of b. pertussis strains with ld50 ranging between 9.0 and 14.1 billion bacteria in 28 (13.43%) were obtained. ... | 1977 | 190070 |
| reaginic antibody production to ascaris suum allergen, asc-1. i. the function of glutaraldehyde-polymerized antigen in the induction of reaginic (ige) antibodies in the rat. | rats immunized with a purified ascaris suum allergen (asc-1) or with its dinitrophenylated derivate (dnp-asc-1) produced high levels of reaginic (ige) antibodies. a second injection of antigen given 30 days later did not result in an anamnestic ige antibody response. immunization of adult-thymectomized, lethally-irradiated and bone-marrow reconstituted (atxb) rats with soluble asc-1 or dnp-asc-1 failed to stimulate reaginic antibody production. the administration of glutaraldehyde-polymerized an ... | 1977 | 301862 |
| the effects of basic pancreatic trypsin inhibitor (bpti) incorporated in freund's complete adjuvant and in freund's incomplete adjuvant containing bordetella pertussis organisms (per). suppressive effect on cell mediated response to purified protein derivative and competing effect on antibody formation to per in guinea pigs. | 1977 | 304499 | |
| antibody-forming potential of lymph nodes in aged mice, with special reference to the influence of adjuvant. | the secondary antibody-forming potential of non-splenic lymphatic tissues during senescence was investigated in nmri/han mice, both at the cellular and humoral levels. the mean life span of conventionally reared nmri/han mice amounts to 19.86 months. after primary immunization of aged (20-month-old) nmri mice with 4 x 10(8) sheep erythrocytes (se) by the intraperitoneal (i.p.) route, the primary antibody-forming potential of both spleen and lymph nodes was significantly reduced, as compared to y ... | 1977 | 318586 |
| immune response in mice to hapten conjugated sepharose. | mice were injected intraperitoneally with sepharose 4b beads coupled with hapten nip, and their anti-nip response was studied by counting antibody forming cells and determining serum titers. mice responded well to doses of 0.7 ml of packed beads but 0.3 and 1.2 doses induced much weaker responses. anti-nip titers in recipients of 0.7 ml of the antigen lasted nearly constant for at least 7 weeks. both t cell status of the recipient and use of adjuvant had an effect on the response. antigen withou ... | 1977 | 320823 |
| effects of histamine-sensitizing factor and cortisol on lymphocyte adenyl cyclase responses. | levels of cyclic adenosime monophosphate (camp) in lymphocytes are regulated by beta-adrenergic agonists and pge1. the effect of these agonists is potentiated by cortisol. incubation of lymphocytes with histamine-sensitizing factor (hsf) leads to loss of response to epinephrine and pge1. the response can be partially restored by cortisol. incubation of lymphocytes with beta-adrenergic antagonists such as propranolol leads to the loss of beta-adrenergic agents but not pge1. the inhibition by prop ... | 1977 | 833377 |
| ir-gene control of antibody class production. | the antibody response in mice to dnp-insulin is under ir-gene control. the ir gene defects in two strains have been analyzed. in both cases the igg immune response was impaired whereas the igm response was not affected. one h-2 gene haplotype was characterized by lack of igg response, independent of the immunization protocol. a second h-2 haplotype manifested a low response of igg after immunization with bordetella pertussis as an adjuvant but a high response after complete freund's adjuvant. it ... | 1977 | 839064 |
| passive cutaneous anaphylaxis and vasoactive amine challenge in nude (nu/nu) mice. a comparison with nu/+, axenic and young normal animals. | recent suggestions of a thymic origin or thymo-dependent differentiation of tissue mast cells prompted an investigation in the athymic nude (nu/nu) mouse. the outbred nu/nu examined were found to possess mast cells in at least comparable numbers to the phenotypically normal nu/+. these nu/nu were superior to nu/+ as recipient for mast cell-dependent, long latent period (ige-type), passive cutaneous anaphylactic (pca) reactions. a variety of studies performed, including competition with nu/+ seru ... | 1977 | 844890 |
| erythromycin prophylaxis for pertussis. | a neonate admitted to the newborn nursery was found to have bacteriologically proven bordetella pertussis whooping cough, and two of seven infants exposed to this child became infected. erythromycin administration to the seven infants apparently prevented further infection as well as the appearance of clinical disease in the two infected infants. this limited experience supports previous reports of the efficacy of erythromycin in preventing b. pertussis infection and disease in susceptible child ... | 1977 | 850603 |
| prevention of homocytotropic antibody formation and anaphylactic sensitization by prefeeding antigen. | studies were conducted in rats to determine whether pre-feeding antigen can prevent the development of bordetella pertussis-induced homocytotropic antibody (hca) formation and anaphylactic sensitization to the fed antigen. da rats fed ragweed for a minimum of 2 wk and sprague-dawley rats fed horse serum for at least 4 wk demonstrated specific unresponsiveness to anaphylactic sensitization as measured by their inability to form hca and their normotensive response to intravenous challenge with ant ... | 1977 | 893876 |
| evidence for suppressor cells in lewis rats' experimental allergic encephalomyelitis. | in this work we demonstrate a suppressive activity on the induction of experimental allergic encephalomyelitis (eae) in lewis rats, transferable to syngeneic animals, challenged with encephalitogenic mixture (myelin basic protein, complete freud's adjuvant plus bordetella pertussis organisms) 24 h later. this activity is probably effected by t cells and not by (an) inhibitory serum factor(s). the induction of this specific protection could be due to the penetration of the myelin basic protein an ... | 1977 | 923633 |
| adjuvant effect of bordetella pertussis vaccine to sheep erythrocytes in mice: enhancement of cell-mediated immunity by subcutaneous administration of adjuvant and antigen. | the subcutaneous route (s.c.) was used to study the adjuvant effect of bordetella pertussis vaccine (pv) on cell-mediated immunity to sheep erythorcytes (srbc). the immune response was measured by a sensitive assay procedure in which the antigen is injected intracutaneously into the mouse ear and the inflammatory swelling is measured with calipers. pv significantly enhanced cell-mediated immunity to srbc, and the enhancement persisted for at least 3 weeks. pv administered up to 6 days before srb ... | 1977 | 924677 |
| tumour-associated inhibition of immediate hypersensitivity reactions in mice. | the intensity of anaphylactic shock was lower in c3h mice carrying a methylcholanthrene-induced tumour (mcc3) than in their normal counterparts when immunized with ovalbumin and challenged i.v. after 14 days. this tumour-associated inhibitory effect on active systemic anaphylaxis was exerted mainly on events occurring after homocytotropic antibody synthesis because the serum titres of these antibodies were comparable in normal and tumour-bearing animals. in addition, passive systemic anaphylacti ... | 1977 | 405315 |
| a comparative study of the anti-allergic effects of disodium baicalein 6-phosphate (bps) and disodium cromoglycate (dscg). | a comparative study was carried out on the effects of a soluble derivative of baicalein, disodium baicalein 6-phosphate (bps) and disodium cromoglycate (dscg) on the immediate type allergic reactions. bps not only inhibited reaginic antibody-mediated reactions including antigen-induced mediator release from monkey lung, homologous pca in rats, and reaginic antibody-mediated degranulation of mast cell, but also non-reaginic antibody-mediated reactions such as mediator release from guinea pig lung ... | 1977 | 405519 |
| chemical modification of crude timothy grass pollen extract. iii. the effect of glutaraldehyde-induced aggregation on antigenic and immunogenic properties. | timothy pollen extracts have been reacted with glutaraldehyde under conditions leading to different degrees of aggregation of the product. aggregation tends to enhance the previously demonstrated effects of glutaraldehyde in that reactivity with human ige antibody, and ability to induce ige antibody in the bordetella pertussis-treated rat, are further reduced. ability to induce igg antibody with specificity for unmodified extract is substantially retained in all aggregated products. | 1977 | 406207 |
| production of guinea pig reaginic antibody against the house dust mite extract, dermatophagoides pteronyssinus, without adjuvant. | guinea pigs of hartley strain were sensitized with an extract of the house dust mite, dermatophagoides pteronyssinus. positive immediate skin reactions were obtained in more than half of the animals 6 weeks after repeated intradermal injection. adjuvants of a1(oh)3 gel or bordetella pertussis vaccine did not significantly enhance the production of reaginic antibody. prausnitz-küstner type skin test titers were even higher in guinea pigs sensitized with the mite extract without adjuvant. circadia ... | 1977 | 599718 |
| histamine hypersensitivity in mice induced by concanavalin a. | this study compared the responses of cfw and cfi mice to concanavalin a (con a) and the histamine-sensitizing factor (hsf) of bordetella pertussis. there were marked similarities between these two agents with regard to systems implicated in induced histamine sensitivity. con a, like hsf, induces the sensitivity in cfw but not in cfi mice. the sensitizing agents both require the same time for optimum sensitization, both induce cutaneous sensitivities to histamine, and the mice are protected from ... | 1978 | 621347 |
| reversion of dextran sulfate-induced loss of antibacterial resistance by bordetella pertussis. | parenteral injection of dextran sulfate 500 (ds 500; 50 mg/kg of body weight) into mice caused a complete loss of resistance to a sublethal (2 x 10(3) to 5 x 10(3)) infection with listeria monocytogenes. such loss could be prevented by pretreatment of animals with 3 x 10(9) heat-killed bordetella pertussis organisms (po) 5 to 30 days before the administration of ds 500. the increased phagocytic capcity induced by po was only exhausted when a fourfold dose of ds 500, effecting complete loss of an ... | 1978 | 640739 |
| the effects of d-pencillamine and levamisole on leucocyte chemotaxis in the rat. | the administration of d-penicillamine (25 mg/kg) or levamisole (5 mg/kg) had no effect on leucocyte emigration into the exudates formed in inert sponges implanted in normal rats. 2 in rats, previously sensitized to bordetella pertussis and implanted with sponges containing pertussis vaccine, an increased leucocyte migration into the exudates occurred; this was significantly enhanced by the administration of the drugs. 3 neither drug in vitro affected the chemotaxis of rat polymorphonuclear leuco ... | 1978 | 647156 |
| bordetella pertussis in the national capital region. | 1978 | 647536 | |
| acute hemolytic anemia related to diphtheria-pertussis-tetanus vaccination. | three infants developed severe hemolytic anemia following the second or third diphtheria-pertussistetanus vaccination. direct antiglobulin tests were positive, and the infant most severely affected also had reduced serum complement levels, indicating an immunological mechanism for the hemolysis. the presence of igm on the erythrocytes from 2 of the infants could be demonstrated by antiglobulin tests or immunization experiments. heat eluates of the erythrocytes from one of the infants contained a ... | 1978 | 654911 |
| [experimental asthma in rats, and the effect of n (3', 4'-dimethoxycinnamoyl) anthranilic acid (n-5') (author's transl)]. | although guinea pigs have been frequently used as a model of asthma, antibodies produced in this species are generally gamma1 and gamma2 and belong to igg. the antibody responsible for asthmatic attacks in humans is ige, and such is quite different from gamma1 and gamma2, immunologically. guinea pigs are not therefore an adequate model for investigating anti-asthmatic drugs which inhibit ige-mediated mediator release, such as disodium cromoglycate. on the other hand, rats do produce an antibody ... | 1978 | 711029 |
| course of lymphocytic choriomeningitis (lcm) virus infection in suckling mice treated with bordetella pertussis vaccine. | death occurred earlier and the mortality rate was higher in one and two-week-old mice pretreated with bordetella pertussis vaccine and infected intracerebrally with lcm virus, than in not pretreated animals of the same litter. pertussis vaccine treatment contributed to the course of lcm virus infection ending in lethal meningitis in suckling mice, by accelerating the development of their cellular immune response. | 1978 | 754519 |
| islet activating protein (iap) derived from the culture supernatant fluid of bordetella pertussis: effect on spontaneous diabetic rats. | the early phase of insulin secretion to an oral glucose load was blunted in spontaneous diabetic rats. the blunted insulin secretion was associated with markedly impaired glucose tolerance. a single injection of the islet activating protein (iap), a protein derived from the culture medium of bordetella pertussis, into the spontaneous diabetic rats normalised glucose tolerance. the increase in insulin response to glucose was an important contributing factor to the improvement of glucose tolerance ... | 1978 | 348542 |
| formation of biologically active protein from the subunits of islets-activating protein (iap), a new protein isolated from bordetella pertussis. | based on the finding reported in the preceding paper (kanbayashi, et al.: j. biochem) that subunits of islets-activating protein (iap), a new protein purified from the culture media of bordetella pertussis, were inactive as such, but regained the original biological activities when recombined, the conditions required for recovery of the biological activities were studied. essentially the same biological activities as the native iap were recovered when the smallest subunit, f-3, was incubated wit ... | 1978 | 359541 |
| influence of vaccination with bordetella pertussis cells on haemopoiesis in sublethally irradiated mice and their radiation lethality. | 1978 | 217243 | |
| colony formation by subpopulations of human t lymphocytes. i. effects of phytohaemagglutinin and lymphocytosis-promoting factor from bordetella pertussis. | 1978 | 217562 | |
| subcellular fractions for immunizing against pertussis. | differential centrifugation was used to prepare fractions from broken cells of bordetella pertussis strain 114. whole cells and several fractions were then assayed for potency and for safety. crude ribosomal fractions were uniformly protective. however, ribosomes purified by washing in high salt solution and recentrifugation were at least 40 fold less potent. protective antigen was found in the wash fluid. wash fluid was subjected to sds-polyacrylamide gel electrophoresis. no specific protein or ... | 1978 | 223910 |
| polymorphonuclear leukocyte-inhibitory factor of bordetella pertussis. i. extraction and partial purification of phagocytosis- and chemotaxis-inhibitory activities. | a new factor that inhibited phagocytosis to opsonized targets and chemotaxis of pmn was extracted from b. pertussis cells, and named pmn-inhibitory factor (pif). cells in phase i produced 10 times more pif than those in phase iii, and like other phase i-associated components--the hemagglutinin, the histamine-sensitizing factor and agglutinogens--pif showed degenerative, phenotypic variation during in vitro culture of phase i bacteria. pif was partially purified by four steps, including adsorptio ... | 1978 | 224858 |
| bordetella pertussis in a partially immunized host: case report. | 1978 | 96376 | |
| [immune response to tetanus toxoid in mice receiving protein deficient diet immediately after weaning]. | the humoral immune response to tetanus toxoid is evaluated by passive haemagglutination test in mice receiving low diet immediately after weaning during 15 and 30 days. the results show that the more deficient mice give the best antitoxin titers but after the challenge the antibody immune response become proportional to the protein restriction. in other respects and antitoxin titers are higher in 30 days restricted mice than in 15 days deficient ones. the effect of bordetella pertussis adjuvant ... | 1978 | 122306 |
| [proliferation processes in the spleen following administration of bordetella pertussis]. | 1978 | 218390 | |
| [search for new strains of bordetella pertussis for vaccine production]. | 1978 | 220475 | |
| islets-activating protein (iap) in bordetella pertussis that potentiates insulin secretory responses of rats. purification and characterization. | 1978 | 203574 | |
| biological properties of islets-activating protein (iap) purified from the culture medium of bordetella pertussis. | the biological activities were studied of a new protein, islets-activating protein (iap), purified from the culture medium of bordetella pertussis. rats injected intravenously with 1 microgram of purified iap exhibited markedly enhanced insulin secretory responses to glucose, glucagon, epinephrine, and sulfonylureas over a period from 3 to 10 days after the injection. the degree and duration of the enhancement were proportional to the dose of iap; the maximal effect induced by 1-2 microgram of i ... | 1978 | 203575 |
| the role of antibiotics, immunizations, and adenoviruses in pertussis. | sixty-five patients with pertussis were identified by a clinical criterion, and bordetella pertussis was isolated from 75% of these patients or their symptomatic household contacts. negative nasopharyngeal cultures were usually associated with either a history of antibiotic therapy with erythromycin or tetracycline (two of three patients), two or more diphtheria and tetanus toxoids with pertussis (dtp) vaccines (six of eight patients), or both (two of three patients). erythromycin therapy result ... | 1978 | 204899 |
| reactions after pertussis vaccine: a manufacturer's experiences and difficulties since 1964. | pertussis vaccines vary in quality, safety, and efficacy according to the production strains of bordetella pertussis, the method of manufacture, and quality control procedures. it is therefore not justifiable to combine information on the incidence, nature, and severity of reactions after all manufacturers' pertussis vaccines as if they were a single product. attempts were made to collect information on all suspected cases of severe reactions that occurred after administration of about 15 millio ... | 1978 | 205305 |
| a novel type of endotoxin structure present in bordetella pertussis. isolation of two different polysaccharides bound to lipid a. | the endotoxin of bordetella pertussis was cleaved by mild acidic hydrolysis to yield a polysaccharide (polysaccharide i, 15%), a glycolipid (63%) and lipid x (2%). further treatment of the glycolipid with stronger acid released a second polysaccharide (polysaccharide ii, 9%) and material similar to lipid a present in enterobacterial endotoxins. both polysaccharides possess a single molecule of 3-deoxy-2-octulosonic acid as the reducing, terminal sugar. in polysaccharide ii the octulosonic acid i ... | 1978 | 205414 |
| role of complement in the pathogenesis of experimental autoimmune myasthenia gravis. | an acute phase of experimental autoimmune myasthenia gravis (eamg) occurs transiently early in the immune response of lewis rats to nicotinic acetylcholine receptors (achr) when bordetella pertussis is used as adjuvant. it is characterized by a destructive cellular attack directed at the postsynaptic membranes of muscle. acute eamg can be passively transferred to normal rats by igg from serum of rats with chronic eamg. in the present study, acute eamg, induced either by passive transfer of synge ... | 1978 | 206648 |
| [influence of killed bordetella pertussis cells on the resistance against infection with listeria monocytogenes (author's transl)]. | the influence of killed bordetella pertussis cells (b.p.) on the cell-mediated resistance of mice against infection with virulent germs of listeria monocytogenes has been studied. resistance of mice was decreased, when 3 x 10(9) b.p. were injected 1 day before, simultaneously with or 1 day after infection, resulting in augmented amounts of viable listeriae recovered from the spleens 3 days after infection (figure 1). the ld50 was strongly reduced (table 1). transfer of immune spleen cells to rec ... | 1978 | 207050 |
| modulation of the antibody response to sheep erythrocytes in murine spleen cell cultures by a t cell mitogen extracted from bordetella pertussis. | 1978 | 207953 | |
| regulation of bordetella pertussis extracytoplasmic adenylate cyclase. | 1978 | 208400 | |
| machakos project studies. agents affecting health of mother and child in a rural area of kenya. v. pertussis sentypes in kenyan children 1974--1975. | cultures of bordetella pertussis were isolated by pernasal swabs from kenyan children in whom whooping-cough was suspected. serotyping of 94 of these isolates was undertaken by four laboratories in europe, and there was very close agreement in their typing results. each laboratory found that the incidence of type 1, 3 was the lowest of the three types, and that there were approximately equal numbers of types 1, 2, 3 and 1, 2. no new serotype was found. this distribution of serotypes was found in ... | 1978 | 209590 |
| [antigenic activity of bordetella pertussis during cultivation in liquid nutrient media]. | the authors studied the antigenic activity of bordetella pertussis during stab cultivation. for the first time there were revealed changes (oscillations) of antigenic activity: formation of 3 peaks of which the middle was the highest. a possibility of improving the quality of pertussis vaccine by using biomass at the height of the peak was experimentally founded. | 1978 | 209647 |
| on the relationship between inflammation and altered camp metabolism in lungs of b pertussis-vaccinated mice. | bordetella pertussis-vaccinated mice were examined for evidence of inflammation. using polymorphonuclear leukocyte and fluid accumulation as markers, inflammation was evidenced in the lungs and to a lesser extent in the livers of such mice. both heart and kidney showed no evidence of inflammation. development of the inflammatory lesion followed a time course similar to that previously reported for increased sensitivity to histamine-mediated camp accumulation. this close parrallelism between infl ... | 1978 | 210126 |