Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| human papillomavirus-associated anogenital neoplasia in persons with hiv infection. | 1992 | 1318737 | |
| early promoters of genital and cutaneous human papillomaviruses are differentially regulated by the bovine papillomavirus type 1 e2 gene product. | the physical state of the human papillomavirus (hpv) genome is usually different in malignant lesions of the skin, in which it is generally found in episomal form, and genital mucosa, in which it is frequently integrated with disruption of the e2 gene. using chimeric or natural hpv promoters in the presence of the bovine papillomavirus type 1 e2 gene product, we observed transcription activation or repression, depending on the distance of e2-binding motifs from the start site. we found a clear d ... | 1992 | 1318941 |
| trichomonas vaginalis (tv) and human papillomavirus (hpv) infection and the incidence of cervical intraepithelial neoplasia (cin) grade iii. | the temporal relationship between cervical infection with trichomonas vaginalis (tv) or human papillomavirus (hpv) and the incidence rate of cervical intraepithelial neoplasia grade three (cin iii) was examined in a cohort of 43,016 norwegian women. from 1980 to 1989, a cervico-vaginal infection from tv and hpv was diagnosed cytologically in 988 and 678 women, respectively. during the 181,240 person-years of observation, 440 cases of cin iii/cervical cancer developed. the age-adjusted incidence ... | 1992 | 1319218 |
| human papillomavirus and herpes virus dna are not detected in benign and malignant prostatic tissue using the polymerase chain reaction. | fresh prostatic tissue removed at the time of surgery was assayed for the presence of human papillomavirus (hpv) types 6, 11, 16, 18 and 33 and herpes and varicella-zoster viruses (hv) using dna amplification followed by specific hybridization. thirty samples representing both benign and malignant prostatic disease were assayed. although appropriate amplimers were present for beta globulin gene indicating successful extraction of dna, no hpv or hv amplimers could be obtained with appropriate pri ... | 1992 | 1319507 |
| [virus and cancer]. | there are at least four viruses tightly associated with human cancer: htly-i and htly-ii with certain leukemias, ebv with lymphomas, bhv with hepatocarcinomas and hpv with genital cancer. in this work we discuss some evidences indicating these associations; in particular we emphasize the characteristics of human papillomavirus (hpv), due to its growing importance in the development of uterine-cervix carcinoma and the mortality of mexican women. the low percentage of infected individuals that dev ... | 1992 | 1319613 |
| detection of human papillomavirus type 16 dna sequences in archival cervical tissues by the polymerase chain reaction. | we have evaluated the polymerase chain reaction for the detection of viral dna sequences in paraffin-embedded archival tissues. in 63 frozen cervical biopsy specimens that were taken from premalignant and invasive lesions, southern blotting detected human papillomavirus (hpv) type 16 dna in 28 (44%) of the samples. in the polymerase chain reaction analysis of the formalin-fixed, paraffin-embedded mirror biopsy specimens, 46 (73%) of the tissues were found to be positive for hpv type 16. in three ... | 1992 | 1319702 |
| detection of human papillomavirus dna sequences in cervical lesions by in situ hybridization using biotinylated dna probes. | ninety-six biopsy specimens were collected for studying the association of specific sequences of hpv dna with genital warts, precancerous lesions and invasive cervical cancer. using dna probes labeled with biotin, 17 of the 22 (77.27%) specimens showing condyloma and 1 of the 14 (7.14%) specimens displaying subclinical papillomavirus infection (spi) of cervix were found to be positive for hpv 6/11; 4 of 23 (17.39%) specimens of squamous epithelial cervical cancer and 2 of the 23 (8.70%) specimen ... | 1992 | 1319885 |
| histological human papillomavirus induced lesions: typization by molecular hybridization techniques. | the viral typization in human papillomavirus (hpv) infections of the lower female genital tract is relevant both from the epidemiological and the clinical point of view. we have tested dna from specimens obtained by guided biopsies on neoplastic and benign lesions, using single probes of the different virus types (6, 11, 16 and 18) by dot-blot and southern blot analysis. according to previous studies hpv 16 and 18 have been detected in neoplastic lesions, while 6 and 11 were more frequently foun ... | 1992 | 1319902 |
| prevalence of human papillomavirus infections among heterosexual men and women with multiple sexual partners. | a prospective study of 65 men and 111 women with multiple heterosexual partners was designed to assess the prevalence and potential risk factors of genital human papillomavirus (hpv) infections. in addition, the hpv reservoir in genital, rectal, and oral mucosa was examined. the specimens for the detection of hpv dna were taken from different sites such as the urethra and coronal sulcus (men), cervix and labia minora (women), anus, rectum, tongue, and buccal mucosa (both men and women). women un ... | 1992 | 1320094 |
| abnormal papanicolaou smears. comparison of cytology, colposcopy and cervical swab dna hybridization. | cervical swab sampling for dna hybridization was performed on 89 women attending a colposcopy clinic. in situ hybridization studies for human papillomavirus (hpv) types 6/11, 16/18 and 31/33/35 were performed on the specimens. biopsy specimens were obtained from 65 patients. the cytologic, colposcopic and histologic results were classified into five groups and assigned a score of 0-5, depending upon the diagnosis. an increasing score correlated positively with the presence of hpv, especially typ ... | 1992 | 1320120 |
| genital human papillomavirus infection. | 1992 | 1320240 | |
| etiology of breast carcinoma: no apparent role for papillomavirus types 6/11/16/18. | a recent study has shown that human papillomavirus (hpv) types 16 and 18 can immortalize normal breast epithelium, and raised the possibility that hpv may be etiologically related to some cases of breast cancer. in order to investigate this possibility, we performed polymerase chain reaction (pcr) assays for hpv types 6, 11, 16 and 18 in 15 papillomas, 15 papillary carcinomas, and 13 infiltrating ductal carcinomas of the breast. no hpv-related dna sequences were identified by southern blotting o ... | 1992 | 1320761 |
| detection of multiple types of human papillomavirus in a giant condyloma from a grafted patient. analysis by immunohistochemistry, in situ hybridisation, southern blot and polymerase chain reaction. | immunosuppressed patients such as transplant recipients are known to develop multiple lesions suggestive of human papillomavirus (hpv) infection. a giant anal condyloma was obtained from a transplant patient; several fragments taken from different areas were examined for the presence of hpv dna using in situ hybridisation, polymerase chain reaction (pcr) and southern blot. typical koilocytes were seen in routinely stained tissue sections, suggesting an hpv infection; furthermore, group specific ... | 1992 | 1320793 |
| general primer polymerase chain reaction in combination with sequence analysis for identification of potentially novel human papillomavirus genotypes in cervical lesions. | we recently described the detection of potentially novel human papillomaviruses (hpv) genotypes (hpv types x [hpv x]) in cervical smears (a. j. c. van den brule, c. j. l. m. meijer, v. bakels, p. kenemans, and j. m. m. walboomers, j. clin. microbiol. 28:2739-2743, 1990) by using the general primer-mediated polymerase chain reaction method (gp-pcr). in this study, the hpv specificities of gp-pcr products were determined by sequence analyses. m13 bacteriophage clones of pcr products derived from c ... | 1992 | 1321168 |
| geographical dependence of sequence variation in the e7 gene of human papillomavirus type 16. | we have determined nucleotide sequences of the e7 open reading frame (orf) of human papillomavirus type 16 (hpv-16) isolates obtained from 32 genital tumours and two hpv-16-transformed human keratinocyte cell lines. in comparison to the prototype hpv-16 isolated from a german cervical cancer biopsy, no sequence variations were noticed in either the two cell lines or the 10 biopsies that were obtained from german patients. in contrast only three of 22 (13.6%) of tanzanian isolates showed the prot ... | 1992 | 1321216 |
| functional interaction of p53 with hpv18 e6, c-myc and h-ras in 3t3 cells. | wild-type (wt) p53 has been suggested to be the product of a tumor-suppressor gene. recently, it has been shown that the e6 oncoproteins of human papillomavirus (hpv) types 16 and 18, like the sv40 large t antigen, are physically associated with wt p53. we have investigated the functional interaction of wt p53 with the viral oncogene products of hpv16 and 18 and with cellular oncogenes by transfection of nih3t3 cells with p53 wt alone or with several oncogene(s). we found that over-expression of ... | 1992 | 1321402 |
| the prevalence of hpv16dna in normal and pathological cervical scrapes using the polymerase chain reaction. | human papillomavirus (hpv) has been implicated in the development of cervical cancer. newly developed molecular techniques have facilitated hpv identification and typing of cervical lesions. several studies have suggested a strong correlation between hpv16 and intraepithelial cervical neoplasia. however, there is no agreement on this point since hpv16 is detected also in normal cervical tissue. we have amplified the l1 region of hpv16 dna using the polymerase chain reaction (pcr) technique and a ... | 1992 | 1321780 |
| retinoblastoma protein binding properties are dependent on 4 cysteine residues in the protein binding pocket. | the retinoblastoma gene product (prb) participates in regulating mammalian cell replication. the mechanism responsible for prb's growth regulatory activity is uncertain. however, prb is known to bind viral transforming proteins including the papilloma virus e7 protein, cellular proteins, and dna. prb contains a critical domain termed the "binding pocket" which is required for binding activities. this binding pocket contains 8 cysteine residues. a naturally occurring mutation affecting one of the ... | 1992 | 1321820 |
| the status of the p53 gene in human papilloma virus positive or negative cervical carcinoma cell lines. | we have analyzed p53 gene alterations in five cervical cancer derived cell lines. two of the five cervical cancer cell lines, htb31 (c-33a) and 32 (ht-3), harbored missense mutations in codons 273 and 245 respectively, whereas the other three tumor cell lines, htb33 (me180), 34 (ms751) and 35 (siha), did not reveal any mutation in the p53 coding sequence spanning codons 126-307. although all the tumor cell lines express comparable levels of p53 rna, only htb31 and htb32 contain high or detectabl ... | 1992 | 1322252 |
| immortalization of fetal mouse brain glial cells by human papillomavirus type 16 e7 genes. | fetal mouse brain glial cells in primary cultures were immortalized by recombinant retroviruses containing human papillomavirus type 16 e7 genes, and named vr-2g cells. the presence and expression of e7 genes in vr-2g cells were demonstrated by the southern and the northern blot analyses. vr-2g cells did not form colonies in soft agar culture, indicating that vr-2g cells have no transforming phenotypes. by the karyotype analysis, vr-2g cells consisted of two cell populations, the pseudo-diploid ... | 1992 | 1322802 |
| human papillomavirus (hpv) type 16 and 33 e6/e7 region transcripts in tonsillar carcinomas can originate from integrated and episomal hpv dna. | this study was undertaken to determine whether human papillomavirus (hpv) e6/e7 gene transcription in tonsillar carcinomas is correlated with viral dna integration. therefore, tonsillar carcinomas containing hpv-16 (n = 2) and hpv-33 (n = 2) dna were analysed for the viral physical state and transcription of the e6/e7 region. southern blot analysis, dna polymerase chain reaction (pcr) and, eventually, two-dimensional gel electrophoresis revealed indications for the presence of only episomal dna ... | 1992 | 1322962 |
| [value of the in situ hybridization technique in the diagnosis of human papilloma virus infections of the uterine cervix. correlations between the human papilloma virus type and the morphological features]. | clinico-pathologic, epidemiologic and molecular analytic studies have shown that: i) the squamous cancer of the uterine cervix derives from intraepithelial lesions with different degrees of atypia; ii) human papillomavirus (hpv) can play a fundamental role in the progression of these lesions. we have examined 219 uterine cervix biopsies by means of the in situ hybridization technique (ish) using biotinylated probes for 6/11, 16/18 and 31/35/51 hpv strains. the histologic lesions were as follows: ... | 1992 | 1323097 |
| correspondence re: r. a. wolber and p. b. clement, in situ dna hybridization of cervical small cell carcinoma and adenocarcinoma using biotin-labeled human papillomavirus probes. mod pathol 4:96, 1991. | 1992 | 1323114 | |
| oral condyloma lesions in patients with extensive genital human papillomavirus infection. | the incidence, location, and morphologic appearances of human papillomavirus oral lesions in patients with genital condylomatosis were investigated with clinical, colposcopic, and histologic examination as diagnostic procedures. the human papillomavirus types were also evaluated with filter in situ hybridization. | 1992 | 1323211 |
| conserved cysteine residue in the dna-binding domain of the bovine papillomavirus type 1 e2 protein confers redox regulation of the dna-binding activity in vitro. | the bovine papillomavirus type 1 e2 open reading frame encodes three proteins involved in viral dna replication and transcriptional regulation. these polypeptides share a carboxyl-terminal domain with a specific dna-binding activity; through this domain the e2 polypeptides form dimers. in this study, we demonstrate the inhibition of e2 dna binding in vitro by reagents that oxidize or otherwise chemically modify the free sulfhydryl groups of reactive cysteine residues. however, these reagents had ... | 1992 | 1323841 |
| human papillomavirus infections in the respiratory tract. | 1992 | 1323936 | |
| expression of transforming growth factor alpha mrna in benign and malignant tissues derived from gynecologic patients with various proliferative conditions. | growth factors are polypeptides involved in the regulation of normal and malignant cell growth. transforming growth factor alpha (tgf alpha) is one of such protein growth factors that plays an important role in the regulation of mammalian cell growth. in this study, the expression of tgf alpha mrna was studied in tissue specimens obtained at the time of surgery from patients with benign and malignant gynecologic proliferative conditions. to analyze tgf alpha mrna expression we utilized the highl ... | 1992 | 1323947 |
| effect on cancer cells of plasmids that express antisense rna of human papillomavirus type 18. | some human squamous cell carcinomas contain dna of human papillomaviruses (hpv) and express rna from the e6 and e7 genes. we have examined the effect of plasmids that express antisense rna of these genes on the growth of the human cancer cell lines hela, c4-1, and 1483, which contain hpv type 18 dna. as controls, the human cancer cell line 183 and the vero line of monkey kidney cells were used, which do not contain hpv. plasmids were introduced into the cells by electroporation; cells that conta ... | 1992 | 1324791 |
| occurrence of p53 gene deletions and human papilloma virus infection in human head and neck cancer. | little is known regarding the molecular genetic events in head and neck carcinoma. epidemiological evidence suggests that both alcohol and tobacco use are related to the development of these neoplasms, and viral infections have also been postulated to play a role in some tumors. loss of p53 tumor suppressor gene function has been found in many malignancies and can occur through either gene mutation or by interaction with the e6 protein of oncogenic human papilloma viruses (hpv). because the muco ... | 1992 | 1324797 |
| the detection of human papillomaviruses in cervical biopsies by immunohistochemistry and in situ hybridization. | the presence of human papillomavirus (hpv) types 6, 16 and 18 in cervical biopsies can be detected by an immunoperoxidase technique using type-restricted monoclonal antibodies raised against fusion proteins representing the l1 major capsid proteins of these three hpv types. in a retrospective study (n = 54) we have used these antibodies and biotinylated dna probes of hpv 6, 16 and 18 to detect and type hpv in formalin-fixed material from the cervix. the biopsies were classified histologically in ... | 1992 | 1325072 |
| long term follow up of women with borderline cervical smear test results: effects of age and viral infection on progression to high grade dyskaryosis. | to follow up and assess the significance of borderline change in cervical smears. | 1992 | 1325232 |
| human papillomavirus identified by nucleic acid hybridization in concomitant nasal and genital papillomas. | presence of human papillomavirus (hpv) as the etiologic agent in nearly all upper respiratory tract recurrent papillomas is well-established. the technique of nucleic acid hybridization now allows specific typing of hpv with a high degree of accuracy. this article reports a series of nine consecutive patients treated for nasal papillomas over the past 9 years. eight of these patients had a personal history of genital papillomas (seven patients) or exposure (one patient). with the use of in situ ... | 1992 | 1325584 |
| specific genetic analysis of microscopic tissue after selective ultraviolet radiation fractionation and the polymerase chain reaction. | a method using selective ultraviolet radiation fractionation followed by polymerase chain reaction (pcr) can analyze specific cell subsets present on a microscope section. direct ultraviolet radiation of fixed and stained tissue sections prevents subsequent amplification by pcr. an "umbrella" or dot placed physically over small numbers of pure cell populations selected by microscopic examination protects these cells from the ultraviolet inactivation. the dna in these protected cells can be speci ... | 1992 | 1325739 |
| clinical course of cervical human papillomavirus lesions in relation to coexistent cervical infections. | a prospective follow-up of 530 women with cervical human papillomavirus (hpv) infection was conducted from 1981 to the present (mean 62.9 months). the patients were examined by pap smears and colposcopy with or without biopsies every sixth month. endocervical swabs were taken for culture of cytomegalovirus (cmv), herpes simplex virus (hsv), and chlamydia trachomatis at each visit. during the follow-up period, 179 of the 530 patients (33.8%) had cervical infection and 351 (66.2%) had no coexisten ... | 1992 | 1326128 |
| analysis of fine-needle aspirates for hpv by pcr may be useful in diagnosis of metastatic gynecologic malignancies. | human papillomavirus (hpv) dna has been shown by molecular hybridization studies to persist in both recurrent and metastatic disease in tumors of the female genital tract. we report here the use of the polymerase chain reaction to identify hpv dna in material from fine-needle aspirates (fna) of recurrent or metastatic lesions to document the primary malignancy arising in the lower genital tract. fine-needle aspirates of suspected recurrent or metastatic tumors were obtained from nine patients wi ... | 1992 | 1326475 |
| detection of antibodies to the e4 or e7 proteins of human papillomaviruses (hpv) in human sera by western blot analysis: type-specific reaction of anti-hpv 16 antibodies. | to determine the cross-reactivity between early (e) proteins of different human papillomavirus (hpv) types, 346 serum samples were tested with e4 and e7 of hpv 16. two hundred and sixteen of them were also tested with hpv 1 e4, 21 with hpv 11 e4 and e7, and 109 with hpv 18 e4 and e7. viral fusion proteins were expressed in escherichia coli and used as antigens in western blot experiments. the sera were obtained from patients with hpv-associated genital lesions or cervical cancer, from renal tran ... | 1992 | 1326715 |
| dot blot hybridization in detection of human papillomavirus (hpv) infections in the oral cavity of women with genital hpv infections. | the presence of human papillomavirus (hpv) types 2, 6, 7, 11, 13 and 16 dna in cytologic scrapings of oral mucosa was studied in 309 women with genital hpv infections. the objective was to test the usefulness of oral mucosal scrapings (3 sequential swabs) in hpv dna detection by dot blot hybridization. based on hybridization with the 32p-labelled alu-repeat probe, most samples contained more than 10(5) cells, which is an adequate number of cells for dot blot hybridization. hybridization with 32p ... | 1992 | 1326738 |
| clinical relevance and evaluation of genitoanal papilloma virus infection in the male. | in the light of current epidemiological data, demonstrating a high prevalence of genitoanal papilloma virus infection (gpvi) existing merely in a subclinical or latent form, ambiguity has emanated on the level of ambition that should be considered optimal for the management of gpvi in the male. this review addresses a pragmatic approach to the problem, with an emphasis on diagnosing and treating overt condylomas causing psychosexual disturbance because of the growth of disfiguring but medically ... | 1992 | 1327056 |
| [uterine cervical carcinoma and human papillomaviruses]. | for many years it has been thought that a significant proportion of cervical cancer could be attributed to sexually transmitted agents, such as sperm, smegma, treponema pallidum, gonococcus and herpes simplexvirus type 2. recent advances of molecular biology, however, have revealed that human papillomavirus (hpv) might be the most causative virus of the disease. since hpv type 16 dna was found in a patient with cervical cancer in 1983, many hpv types have been cloned from cervical cancers, also ... | 1992 | 1327090 |
| human papillomavirus. | in the past year, new data have been published on the molecular biology of human papillomavirus infections and their relationship to cervical neoplasia. as molecular techniques have become more sophisticated and as the molecular knowledge of human papilloma-virus infections has been pursued in greater depth, it is increasingly apparent that this human tumor dna virus is similar to a number of other oncogenic dna viruses that have been described and well studied. these viruses appear to act throu ... | 1992 | 1327250 |
| properties of p53 mutations detected in primary and secondary cervical cancers suggest mechanisms of metastasis and involvement of environmental carcinogens. | primary human papillomavirus (hpv) positive anogenital cancers normally develop without somatic mutation within the p53 gene. in this study, however, we have identified p53 point mutations in metastases arising from hpv positive cervical carcinomas, suggesting that acquisition of p53 mutation may play a role in the progression of some hpv associated primary cancers. p53 mutants identified in anogenital cancers exhibit a dominant transforming phenotype and increased resistance to hpv16 e6 directe ... | 1992 | 1327751 |
| the predictive value of human papilloma virus (hpv) typing in the prognosis of bronchial squamous cell papillomas. | five solitary squamous papillomas of bronchus with variable degrees of dysplasia, one combined with a laryngeal papilloma and with a neuroendocrine carcinoma in the contralateral lung, and five papillomas combined with invasive squamous cell carcinomas were investigated for their expression of human papilloma virus dna by in situ hybridization. benign squamous cell papillomas showed an association with papilloma virus type 11 and rarely type 6, whereas types 16 or 18, sometimes in combination wi ... | 1992 | 1328017 |
| epidermal growth factor receptor expression and the presence of human papillomavirus in cervical squamous intraepithelial lesions. | to determine the relationship between the expression of epidermal growth factor receptor (egfr) and the presence or type of human papillomavirus (hpv) in cervical squamous intraepithelial lesions (sil), paired colposcopically directed cervical biopsies were obtained from 88 patients referred for abnormal papanicolaou smears. one biopsy was formalin-fixed and processed for conventional light microscopy, and the other was immediately frozen. a portion of the frozen tissue was used for southern blo ... | 1992 | 1328080 |
| human papillomavirus and its prognostic significance in invasive carcinoma of the cervix in young patients. | of 1,200 chinese patients treated for carcinoma of the cervix between 1975 and 1984, those aged 40 years (n = 70) or less had a poorer prognosis. of the latter group, 71% had disease classified as less than international federation of gynecology and obstetrics (figo) stage ib, and in 91% the disease was less than stage iia; however, the 5-year survival of the 45 patients with verified invasive carcinoma was 75% compared with 82% for the overall group. the polymerase chain reaction (pcr) was used ... | 1992 | 1328083 |
| polymerase chain reaction detection of human papillomavirus: quantitation may improve clinical utility. | a case-control study compared detection by polymerase chain reaction (pcr) specific for human papillomavirus (hpv) type 16 with restriction enzyme analysis and southern blot hybridization detection of hpv type 16. cervicovaginal lavage samples from 64 women with histopathologic evidence of a cervical squamous intraepithelial lesion and 55 samples from cytologically healthy women were studied. several methods of pcr product analysis, including radioactive and nonradioactive probing, were compared ... | 1992 | 1328278 |
| [detection of human papillomavirus dna using in situ hybridization]. | 1992 | 1328713 | |
| invasive cervical cancer following cryotherapy for cervical intraepithelial neoplasia or human papillomavirus infection. | to determine the following: 1) the causes for the failure of cervical cryotherapy to prevent cervical cancer, and 2) whether cervical cryotherapy is associated with the development of cervical adenocarcinoma rather than squamous carcinoma. | 1992 | 1328976 |
| second primary cancers following anal and cervical carcinoma: evidence of shared etiologic factors. | the authors examined the incidence of second primary cancers occurring after cervical and anal cancer. data from the connecticut tumor registry for 1935-1988 and eight other us tumor registries for 1973-1988 were used. women with primary invasive cervical cancer had a relative risk of 4.6 (95% confidence interval (ci) 2.4-8.1) for subsequent invasive anal cancer. increased relative risks after cervical cancer were also found for cancers of the oral cavity (relative risk (rr) = 2.2), stomach (rr ... | 1992 | 1329500 |
| [behavior of nucleolar organizers in the normal cervix, human papilloma virus infection and cervico-uterine cancer]. | thirty five cervical biopsies were divided into four groups: 5 cases normal cervix, 10 cases with papillomavirus without atypias (hpvio), 10 cases with human papillomavirus with atypias (hpvia) and 10 cases with squamous carcinoma. with the colloidal silver technique, the proteins associated with nucleolar organizers were stained; they were found to increase significantly in number in the groups studied being lowest in the normal cervix and highest in the biopsies with carcinoma. the number of n ... | 1992 | 1330837 |
| detection of human papillomavirus dna without amplification: prospects for clinical utility. | certain hpv types are major risk factors for the development of cervical and other human cancers. this chapter describes some of the problems which have precluded accurate diagnosis of hpv-related conditions in many studies. despite the confusion, a multitude of reports demonstrate that hpv dna testing has clinical utility, and future investigations should be directed at more accurately delineating its role in human health care. | 1992 | 1330906 |
| detection of human papillomavirus in cervical scrapes by the polymerase chain reaction in relation to cytology: possible implications for cervical cancer screening. | an hpv screening strategy based on general primer-mediated and type-specific pcr is described. a relationship was found between an increasing hpv prevalence rate and a higher degree of cervical dysplasia (up to 100% in cervical carcinoma). based on these prevalence studies in the netherlands and preliminary data showing that progression of cervical lesions is always associated with persistent infection of oncogenic/high-risk hpv types, a cervical cancer screening scheme incorporating pcr-based h ... | 1992 | 1330917 |
| viral co-infections in human papillomavirus-associated anogenital lesions according to the serostatus for the human immunodeficiency virus. | in hiv-infected men, human papillomavirus (hpv) infection is strongly linked with the development of anogenital lesions but is not a sufficient factor to explain the neoplastic transformation of such lesions. we investigated the association between hpv and herpesvirus infections in penile and anal lesions from 54 hiv-seronegative and 54 hiv-seropositive men by means of colposcopy, histopathology and in situ hybridization. our patients showed condyloma acuminata (39%), papular warts (35%) and mac ... | 1992 | 1330931 |
| risk factors for cervical cancer in colombia and spain. | a population-based case-control study of cervical cancer was conducted in spain and colombia to assess the relationship between cervical cancer and exposure to human papillomavirus (hpv), selected aspects of sexual and reproductive behaviour, use of oral contraceptives, screening practices and smoking. the study included 436 cases of histologically confirmed squamous-cell carcinoma and 387 age-stratified controls randomly selected from the general population that generated the cases. the presenc ... | 1992 | 1330934 |
| pre- and posttreatment serum antibody responses to hpv 16 e2 and hsv 2 icp8 proteins in women with cervical carcinoma. | serum antibodies to early proteins of human papillomavirus type 16 (hpv 16 e2 protein) and herpes simplex virus type 2 (hsv 2 icp8) can be measured by elisa. in the serum of 122 newly diagnosed cervical carcinoma patients and age-matched controls, enhanced iga antibody levels to an hpv-16 e2 protein derived peptide no. 245 indicated a 9.5-fold (95% confidence limits 2.8-57.2) relative risk of cervical carcinoma. no significant risk was found with a corresponding hpv 6 e2 peptide or hsv 2 icp8. t ... | 1992 | 1331306 |
| human papillomavirus-negative status and c-myc gene overexpression: independent prognostic indicators of distant metastasis for early-stage invasive cervical cancers. | 1992 | 1331478 | |
| homologous sequences in adenovirus e1a and human papillomavirus e7 proteins mediate interaction with the same set of cellular proteins. | studies of adenovirus e1a oncoprotein mutants suggest that the association of e1a with the retinoblastoma protein (prb) is necessary for e1a-mediated transformation. mutational analysis of e1a indicates that two regions of prb are required for e1a to form stable complexes with the retinoblastoma protein. in addition to prb binding, these regions are necessary for e1a association with several other cellular proteins, including p130, p107, cyclin a, and p33cdk2. here we show that short synthetic p ... | 1992 | 1331501 |
| self-administered home cervicovaginal lavage: a novel tool for the clinical-epidemiologic investigation of genital human papillomavirus infections. | our objective was to evaluate a self-administered cervicovaginal irrigation apparatus (my-pap, medtech, bohemia, n.y.) for the detection of cervicovaginal human papillomavirus infection. | 1992 | 1332474 |
| detection of human papillomavirus dna in the normal cervices of japanese women by the dot-blot (vira pap) method. | exfoliated cervical cells obtained from 9 patients with cervical intraepithelial neoplasia (cin) i and ii, 11 with cin iii, 22 with cervical carcinoma, and 599 with cytologically normal cervices (including 63 pregnant women) were evaluated for the presence of human papillomavirus (hpv) by the dot-blot (vira pap) method. hpv infection was detected in 33.3% of the cin i and ii cases, in 54.5% of the cin iii cases, in 68.2% of invasive cervical carcinoma cases, and in 4.2% of the normal group. the ... | 1992 | 1333184 |
| two cases of plantar epidermal cyst associated with human papillomavirus. | hpv-associated epidermal cysts of the sole (haecs) of the foot have been reported recently in japan in which there is positive staining for papillomavirus antibody in the nuclei of the epithelial cells in the cyst wall and vacuoles in the stratified horny material inside the cysts. a causative association with a newly recognized hpv, hpv 60, has been recorded. the authors report two new cases. the possible mechanism of cyst formation in hpv 60-infected epithelium is discussed. | 1992 | 1333376 |
| tamoxifen stimulates human papillomavirus type 16 gene expression and cell proliferation in a cervical cancer cell line. | the widely adopted use of tamoxifen as a chemotherapeutic agent is primarily based on its inhibition of cancer cell growth. however, we report that tamoxifen at low concentrations (10(-9) and 10(-11) m) causes stimulation of cell proliferation in a cervical cancer cell line, sfr. the facts that sfr cells do not contain estrogen receptors and are estrogen nonresponsive imply the existence of an antiestrogen-specific binding protein and suggest that the effect of tamoxifen is possibly mediated thr ... | 1992 | 1333883 |
| in vitro infection of normal human keratinocytes by human papillomavirus type 1 followed by amplification of the viral genome in reconstructed epidermis. | primary cultures of normal human keratinocytes were inoculated in vitro with human papillomavirus type 1 (hpv-1), the agent responsible for deep plantar warts. upon transfer to dead de-epidermized dermis and growth at the air-liquid interface, keratinocytes reconstituted a pseudoepidermis. under these highly differentiating conditions, hpv-1 dna amplification was found to take place in the reconstructed epidermis, being detectable from 7 days after the transfer and persisting for at least 10 day ... | 1992 | 1335028 |
| prognostic significance of polymerase chain reaction detected human papillomavirus of tumors and lymph nodes in surgically treated stage ib cervical cancer. | this study describes the prognostic role of polymerase chain reaction detected human papillomavirus (hpv) in stage ib cervical cancer patients treated with radical hysterectomy and pelvic and paraaortic node dissection. all tumors were confined to the cervix and all margins and nodes were disease free. twenty-one patients were analyzed: 6 patients recurred within 20 months of initial therapy, while 15 had no evidence of disease with a minimum follow-up of 36 months. polymerase chain reaction (pc ... | 1992 | 1335431 |
| activation of human papillomavirus type 18 e6-e7 oncogene expression by transcription factor sp1. | the human papillomavirus 18 (hpv18) e6 and e7 proteins are considered to be primarily responsive for the transforming activity of the virus. in order to analyse the molecular mechanisms resulting in viral oncoprotein expression, it is necessary to identify the factors involved in the transcriptional regulation of the e6/e7 genes. here we define by gel retardation experiments a sequence aberrant sp1 binding site present in the promoter proximal part of the viral transcriptional control region (up ... | 1992 | 1336181 |
| human papillomavirus detection in cervical cells by in situ hybridization with biotinylated probes. | we have investigated the applicability of human papillomavirus (hpv) dna detection by in situ hybridization with biotinylated probes in epithelial cells obtained from the cervix using a cotton tip swab. we describe a simple procedure for obtaining homogeneous cell samples and good preservation of cellular structure. this is achieved by pretreatment of cells with l-cysteine before hybridization. separate denaturation of cellular dna and probe dna is also necessary for satisfactory results. both b ... | 1992 | 1336676 |
| human papillomavirus in cervix carcinoma and condylomata acuminata--identification of hpv-dna by improved dot-blot-hybridization. | using the benzoylated naphthoylated deae cellulose method (bnd-method) we have designed a more efficient approach for the detection of human papillomavirus-dna (hpv-dna) via dot-blot and hybridization. biopsy material from anogenital warts (40 patients), invasive carcinoma uteri (12 patients) and normal controls (20 patients) were studied for the presence of hpv-dna. phenol extracted dna from representative lesions was loaded onto a pretreated nitrocellulose filter, was incubated under stringent ... | 1992 | 1336719 |
| [the role of colposcopy in the diagnosis of hpv infection]. | human papillomavirus (hpv) has been implicated as an important etiologic factor in cervical carcinoma. this study evaluates the efficacy of colposcopy in the detection of cervical lesions with koilocytosis features. colposcopy, cervical smears and biopsy specimens from 217 women seen in our department between january-december 1991 were examined. the colposcopy examination detected 77.5% of the viral presence the histological diagnosis detected 85% of hpv, but the cytologic smears showed only 52% ... | 1992 | 1337154 |
| detection of human papillomavirus (hpv) dna type 6/11 in a conjunctival papilloma by in situ hybridization with biotinylated probes. | four cases of conjunctival papilloma in two different patients were examined by in situ hybridization for hpv dna type 6/11, 16/18 and 31/33/51. formalin-fixed and paraffin-embedded tissues were hybridized by biotinylated probes. one tumor and one of its recurrences showed nuclear positivity for hpv 6/11 in the superficial cells of the epithelium. the results suggest that hpv type 6/11 may be etiologic agent of conjunctival papillomas. the benign behavior of these neoplasms may be related to the ... | 1992 | 1337200 |
| the absence of genital human papillomavirus dna in virginal women. | our aim was to determine the prevalence of human papillomavirus (hpv) infection in virginal women and to establish if other forms of sexual activity not involving penetration may contribute to the transmission of this infection. female patients attending medical practitioners, and female high school students were recruited. each participant answered an anonymous self answer questionnaire and collected a tampon specimen. tampons were analysed for the presence of human papillomavirus by the polyme ... | 1992 | 1337472 |
| occurrence and expression of human papillomavirus type 16 genes in uterine cervical carcinomas. | the detection of human papillomavirus (hpv) type 16 early genes: e7, e5, and the late gene: l1 was attempted in 42 uterine cervical neoplasia (35 cervical carcinomas and 7 cervical dysplasias) using the polymerase chain reaction (pcr) method. consequently, e7 gene was detected in 19 (54.3%) of 35 carcinomas and in 5 (71.4%) of 7 dysplasias, e5 gene was detected in 7 (20.0%) of 35 carcinomas and in 5 (71.4%) of 7 dysplasias, l1 gene was detected in 18 (51.4%) of 35 carcinomas and in 5 (71.4%) of ... | 1992 | 1338065 |
| epidermodysplasia verruciformis. | epidermodysplasia verruciformis is a rare skin disease characterized by the disseminated, flat, wart-like lesions caused by human papillomavirus and a high frequency of various skin cancers. the clinical aspects, histological findings, genetic and immunological factors, and human papillomavirus types found in the disease and their roles in skin cancers are reviewed. | 1992 | 1338070 |
| non radioactive in situ hybridization for detection of human papilloma virus dna in squamous cell carcinoma of tongue. | previous studies indicate that hpv type 16 and 18 (hpv) are associated with squamous cell carcinoma of the head and neck. in this investigation we evaluate in our hospital 253 patients with oral squamous cell carcinoma of the tongue not related to tobacco or alcohol with a tumor index of t2 no mo between 1981 and 1991. from 12 patients we were able to obtain tissue. for detection of human papilloma virus, dna sequences 6, 11, 16, 18, 31 and 33 in paraffin-embedded human tissue biopsies a non-rad ... | 1992 | 1338425 |
| influence of fixation on human papillomavirus dna detection in frozen and embedded paraffin lesions by in situ hybridization with biotinylated probes. | series of frozen or paraffin-embedded tissues from various body sites, taken from non-immunosuppressed or immunosuppressed patients with persistent papilloma lesions were examined for the presence of group specific antigen from human papillomavirus (hpv) by indirect immunofluorescence or hpv dna by in situ hybridization with biotinylated probes. we have shown that it is possible to detect hpv dna after fixation of tissues in neutral formalin, bouin's or baker's solution. however, the sensitivity ... | 1992 | 1338657 |
| viruses in anogenital cancer. | the association between sexual activity and cancer, first described in carcinoma of the cervix, has been expanded to include the majority of anogenital squamous epithelial carcinomas. current evidence suggests that human papillomavirus (hpv) may be of great importance in the development of these tumours, whilst herpes simplex type 2 virus (hsv-2) and human immunodeficiency virus (hiv) may play minor roles. certain types of hpv dna, including types 16, 18, 31, 33 and 39 are found in most but not ... | 1992 | 1339181 |
| [early anti-hpv 16 protein antibodies in patients with anogenital tumors]. | a study was worked out in female patients with anogenital tumors and in apparently healthy women, aimed at the detection of antibodies against e4 and e7 hpv 16 proteins. as regards the detection of hpv 16 infection, the dna hybridization and western-blot results were in good agreement. the hpv 16 infection was detected in a relatively high number of the patients (17 out of 24) and of the apparently healthy women using western blot. | 1992 | 1339204 |
| inhibition of the transformed phenotype of carcinoma cells that contain human papillomavirus. | 1992 | 1340137 | |
| papillomaviruses in human disease: part i. pathogenesis and epidemiology of human papillomavirus infections. | papillomas of man and animals were shown to contain an infectious agent at the beginning of this century. since then, various attempts were made to isolate the papilloma inducing agent. electron microscopic studies later revealed that the agent is a virus, however all attempts to culture it with conventional virological techniques failed. it took until the development of molecular cloning techniques before a detailed study of this virus could start. today it is clear that the papilloma-associate ... | 1992 | 1341482 |
| leukoregulin and gamma-interferon inhibit human papillomavirus type 16 gene transcription in human papillomavirus-immortalized human cervical cells. | the human papillomavirus (hpv) transforming genes e6 and e7 are retained and expressed in the majority of cervical cancers implying an important role for these proteins in maintenance of the malignant phenotype. leukoregulin (lr) and recombinant gamma-interferon (r-ifn-gamma), lymphokines secreted by immune cells present in regressing hpv infections, inhibited transcription of e6/e7 rnas in several human cervical epithelial cell lines immortalized by recombinant hpv-16, -18, and -33 dnas. r-ifn ... | 1992 | 1345813 |
| hpv-16-related dna sequences in kaposi's sarcoma. | in the usa, kaposi's sarcoma associated with the acquired immunodeficiency syndrome (aids-ks) is ten times more common in homosexual or bisexual men than in heterosexual men with aids. one explanation for this finding is that aids-ks may be caused by an infectious agent. because there is a high incidence of human papillomavirus (hpv) infection, especially hpv-16, in homosexual men, we have sought hpv dna sequences in kaposi's sarcoma. we used the polymerase chain reaction with a primer pair spec ... | 1992 | 1346878 |
| clonal p53 mutation in primary cervical cancer: association with human-papillomavirus-negative tumours. | analyses of cancer cell lines and of anal cancers suggest an inverse correlation between infection with human papillomavirus (hpv) and somatic mutation of the p53 tumour-suppressor gene. we have investigated this association in primary cervical tumours. tumour-tissue samples from 28 women with primary cancer of the cervix were analysed for presence of hpv sequences and for somatic mutations of the p53 gene. southern blot analysis and the polymerase chain reaction (pcr) showed that 25 of the tumo ... | 1992 | 1349102 |
| relationship of human papillomavirus type to grade of cervical intraepithelial neoplasia. | objective--to determine the relationship of human papillomavirus (hpv) type to grade of cervical intraepithelial neoplasia (cin) in a large series of cases. design--a survey of hpv types in cin lesions detected using a new, highly accurate method for typing hpv that is based on restriction fragment length polymorphism analysis of amplimers produced during polymerase chain amplification of the conserved l1 region of hpv using consensus primers. setting--private gynecologists' offices and inner-ci ... | 1992 | 1349360 |
| urological evaluation of sexual partners of women with human papillomavirus (hpv) infection. | cancer of the uterine cervix has been related to hpv infection, based on clinical and laboratory data. the high recurrence rate in couples undergoing treatment for hpv infection points to a probable viral reservoir, either in subclinical lesions or in male internal genital organs. we have evaluated 31 men, all sexual partners of women with hpv infection. eleven patients (35.5%) had related lesions: 4 (12.9%) with condyloma acuminatum; 5 (16.1%) with lesions revealed by magnified examination afte ... | 1992 | 1369626 |
| increased sensitivity of human keratinocytes immortalized by human papillomavirus type 16 dna to growth control by retinoids. | human papillomavirus (hpv) type 16 (hpv16) is associated with a large percentage of cervical malignancies, and hpv16 dna can immortalize human keratinocytes in vitro. the transforming ability of the virus resides primarily in the open reading frames e6 and e7. retinoids are potent modulators of growth and differentiation of keratinocytes and have been shown to reverse cervical lesions resulting from hpv infection. we compared the sensitivity of normal human foreskin keratinocytes (hkc) and four ... | 1992 | 1370074 |
| epitope mapping of the human papillomavirus type 16 e4 protein by means of synthetic peptides. | eight overlapping icosapeptides covering the entire sequence of the e4 protein of human papillomavirus type 16 (hpv-16), were prepared and tested for their reactivity with human sera in igg-specific elisa. the strongest reactivity of sera from hpv-16 dna-positive invasive cervical carcinoma (inca) patients was detected with the peptide denoted 16/e4-6, covering amino acids 51 to 70. subsequently nearly 200 sera were tested for the presence of the 16/e4-6-specific antibody. reactivity was more fr ... | 1992 | 1371544 |
| [cancer of the penis and its treatment]. | it had been believed that carcinoma of the penis was rather rare in the developed countries comparing with that in the under-developing countries, however, the recent epidemiological studies failed to reveal any clear difference of the incidence of carcinoma of the penis all over the world. in these days so called successful treatment is coming to be evaluated by the quality of life (qol) after surgical or nonsurgical treatment (especially sexual function tended to be considered very important f ... | 1992 | 1373451 |
| human papillomavirus e6 proteins bind p53 in vivo and abrogate p53-mediated repression of transcription. | the transforming proteins of dna tumor viruses sv40, adenovirus and human papillomaviruses (hpv) bind the retinoblastoma and p53 cell cycle regulatory proteins. while the binding of sv40 large t antigen and the adenovirus e1b 55 kda protein results in the stabilization of the p53 protein, the binding of hpv16 and 18 e6 results in enhanced degradation in vitro. to explore the effect of viral proteins on p53 stability in vivo, we have examined cell lines immortalized in tissue culture by hpv18 e6 ... | 1992 | 1379175 |
| preferential chromosome loss in human papilloma virus dna-immortalized mammary epithelial cells. | human papilloma virus (hpv) dna-immortalized human mammary epithelial cells may provide a model system for studying the molecular basis of immortalization and its role in breast neoplasia. cytogenetic analyses were performed on clones derived from hpv 16- and hpv 18-immortalized human mammary epithelial cells. the majority of the clones contained near-diploid karyotypes. the single most frequent whole-chromosome loss was that of chromosome 19. regions that showed preference for deletion and/or t ... | 1992 | 1384674 |
| giant condyloma acuminatum in a baby boy. | a giant condyloma acuminatum developed on the penis of a one and a half-year-old japanese boy in two months. the histological features of this tumor were compatible with those of ordinary condyloma acuminatum. although we detected the presence of human papilloma virus (hpv) type 16 by using the polymerase chain reaction system, we could not rule out the possibility that this hpv was present concurrently with other hpv types that cause condyloma acuminatum. the lesion was successfully treated wit ... | 1992 | 1401502 |
| issues concerning women and aids: sexuality. | in the us and globally women are contracting the human immunodeficiency virus (hiv) and developing the acquired immunodeficiency syndrome (aids) the fastest. worldwide, hiv is transmitted primarily through heterosexual intercourse. in the us, the proportion of women who have contracted aids by heterosexual transmission has increased from 11% in 1984 to 34% in 1990. women are at a greater risk than men for transmission by heterosexual intercourse as the ratio of women to men who acquire aids b ... | 1992 | 1408853 |
| recognizing the oral manifestations of aids. | the first sign of hiv infection may be an unusual or rapidly progressive condition of the oral cavity, including malignancies such as kaposi's sarcoma. early diagnosis of these oral conditions can lead to early diagnosis of hiv infection and subsequent treatment with antiretroviral agents that may improve the prognosis. this illustrated review outlines the presenting signs and symptoms of the most common oral manifestations of the aids virus, including hairy leukoplakia, candidiasis, kaposi's sa ... | 1992 | 1449978 |
| hiv disease and aids in women: current knowledge and a research agenda. | the study of the clinical manifestations, progression, and outcome of human immunodeficiency virus (hiv) infection in women has begun in earnest. aids-defining diseases that are more common in women than in men include wasting syndrome, esophageal candidiasis, and herpes simplex virus disease, whereas kaposi's sarcoma is rare. non-aids-defining gynecological conditions such as vaginal candida infections and cervical pathology are prevalent among women at all stages of hiv infection. associations ... | 1992 | 1453325 |
| [four years of screening cervical smears of cervical lesions]. | twelve thousand two hundred and eighty nine pap smears were collected from public hospitals and from private practices during a four year period (january 1987 to december 1990). 4.2% of pap smears exhibited condylomatous or dysplastic lesions. 94.5% of such lesions were encountered in pap smears taken from the transformation zone and which contained endocervical cells. therefore, these smears represent the only adequate sample for cervical cancer screening. in our study, a close concertation bet ... | 1992 | 1469229 |
| laryngeal papillomatosis. | the papilloma is one of the most common benign neoplasms of the larynx. laryngeal papillomatosis is a disease of widespread papilloma formation that most commonly affects the larynx but may involve multiple areas of the aerodigestive tract. typically, onset of disease during childhood is associated with much more aggressive disease. the etiology of this disease is the human papillomavirus. diagnosis is often delayed because the symptoms mimic a variety of other diseases. laryngeal papillomatosis ... | 1992 | 1494048 |
| focal epithelial hyperplasia. | in 2 children (brother and sister) focal epithelial hyperplasia could be diagnosed by means of histology, human papillomavirus typing and southern blot. in the 14-year-old girl the history could be followed over many years. the high familial incidence and the special geographic distribution of focal epithelial hyperplasia are pointed out. | 1992 | 1498399 |
| laser treatment of premalignant and malignant squamous cell lesions of the penis. | thirty men with biopsy-proven premalignant or malignant squamous cell lesions of the penis were treated. all had subclinical aceto-white lesions with histologic evidence for human papilloma virus infection. nineteen patients had penile intraepithelial neoplasia (pin i and ii) and 11 had squamous cell carcinoma. of these 11 patients, 6 had noninvasive penile intraepithelial neoplasia--carcinoma in situ (pin iii/tis)--and 5 had invasive squamous cell carcinoma (4 stage t2 and 1 t3). all were treat ... | 1992 | 1508018 |
| association between hiv infection and cervical neoplasia: implications for clinical care of women at risk for both conditions. | both aids and cervical neoplasia (cn) can result from sexual transmission of hiv infection and may affect similar groups of women. available data on the association between aids and cn have practical implications for gynecological care. we review these data to provide an estimate of the magnitude of the association between cn and hiv infection. | 1992 | 1558715 |
| cone biopsy: a review of 112 cases. | a chart review of 112 patients who underwent cold knife conisation was performed. records showed that 73.5% of the patients smoked cigarettes and 26.5% were using oral contraception. in 85.7% of cases pre-operative cytology/colposcopy findings were within one grade of the cone histology. the majority of lesions were cin iii (59.9%). only 4.5% were normal. human papilloma virus infection was detected histologically in 26.8% of patients. pre-operative punch biopsy was undertaken in only five cases ... | 1992 | 1568845 |
| purification and characterization of a functionally homogeneous 60-kda species of the retinoblastoma gene product. | the retinoblastoma susceptibility gene (rb) encodes a 928-amino acid protein (prb) that is hypothesized to function in a pathway that restricts cell proliferation. the immortalizing proteins from three distinct dna tumor viruses (sv40 large t antigen, adenovirus e1a, and human papilloma virus type 16 e7) have been shown to interact with rb protein through two noncontiguous regions comprised of amino acids 393-572 (domain a) and 646-772 (domain b). we constructed a truncated form of rb (rb p60) t ... | 1992 | 1569054 |
| vulvodynia. diagnostic patterns. | the symptom of vulvar burning demands special evaluation and management. possible factors include complications of therapy, inflammatory dermatoses, lichen sclerosus, and infection with organisms such as candida, human papillomavirus, and herpes simplex virus. vulvar vestibulitis and dysesthetic vulvodynia or pudendal neuralgia have characteristic patterns of discomfort. psychological aspects of vulvodynia include the need for supportive therapy. | 1992 | 1606768 |
| transforming growth factor beta 1 regulation of c-myc expression, prb phosphorylation, and cell cycle progression in keratinocytes. | transforming growth factor beta 1 (tgf-beta 1) is a potent inhibitor of cellular proliferation in a variety of cell types, including skin keratinocytes. tgf-beta 1 suppression of c-myc transcription has been implicated in the mechanism of tgf-beta 1 inhibition of keratinocytes, and evidence suggests that the protein product of the retinoblastoma gene (prb) is a necessary component in this pathway. following growth factor stimulation of quiescent keratinocytes, tgf-beta 1 can inhibit cell cycle p ... | 1992 | 1633111 |
| [laser co2: results of treatment of hpv lesions in male the partner]. | hundred-fifty-seven male partners of patients affected by human papillomavirus (hpv) lesions, underwent co2 laser vaporization between january 1989 and january 1990. the age ranged between 18 and 45 (median 28.2). the vaporization was performed with a coherent system 451 co2 laser equipment, at a power of 16 watt in continuous mode, depth of 2 mm and a free margin of 2 mm. first laser treatment proved successful in 129 patients (82.1%); 17 out of 28 patients with persistent or relapsed pathology ... | 1992 | 1635650 |